amplitaq dna polymerase Perkinelmer Search Results


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  • 99
    Thermo Fisher sybr green pcr master mix
    M-CSF regulates VEGF mRNA transcription through ERK and Sp1. A) Freshly isolated human monocytes were cultured overnight in 5% FBS and subsequently starved for 2 hours in minimal media. The cells were stimulated with 100 ng/ml rhM-CSF for 6 hours followed by isolation of RNA. cDNA was synthesized from total cellular RNA, standardized, and subjected to <t>SYBR</t> Green real-time <t>PCR</t> using primers specific for VEGF-A. The data represents the mean+/−SEM of six individual donors. B) Freshly isolated human monocytes were cultured overnight in 5% FBS and subsequently starved for 2 hrs followed by pre-treatment with either DMSO (vehicle control) or U0126 (10 µM) in minimal media for 30 minutes. The cells were then stimulated with 100 ng/ml rhM-CSF (M-CSF) , (M-CSF+DMSO) , (M-CSF+U0126 10 µM) and analyzed as stated in A. The data represents the mean+/−SEM of three individual donors. C) Freshly isolated human monocytes were cultured overnight in 5% FBS and subsequently starved for 2 hours followed by pre-treatment with either methanol (vehicle control) or mithramycin (200 nM) in minimal media. The cells were then stimulated with 100 ng/ml rhM-CSF (M-CSF) , (M-CSF+MeOH) , (M-CSF+Mith (200 nM)) and analyzed as in A. The data represents the mean+/−SEM of four individual donors.
    Sybr Green Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 101787 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    PerkinElmer dctp
    M-CSF regulates VEGF mRNA transcription through ERK and Sp1. A) Freshly isolated human monocytes were cultured overnight in 5% FBS and subsequently starved for 2 hours in minimal media. The cells were stimulated with 100 ng/ml rhM-CSF for 6 hours followed by isolation of RNA. cDNA was synthesized from total cellular RNA, standardized, and subjected to <t>SYBR</t> Green real-time <t>PCR</t> using primers specific for VEGF-A. The data represents the mean+/−SEM of six individual donors. B) Freshly isolated human monocytes were cultured overnight in 5% FBS and subsequently starved for 2 hrs followed by pre-treatment with either DMSO (vehicle control) or U0126 (10 µM) in minimal media for 30 minutes. The cells were then stimulated with 100 ng/ml rhM-CSF (M-CSF) , (M-CSF+DMSO) , (M-CSF+U0126 10 µM) and analyzed as stated in A. The data represents the mean+/−SEM of three individual donors. C) Freshly isolated human monocytes were cultured overnight in 5% FBS and subsequently starved for 2 hours followed by pre-treatment with either methanol (vehicle control) or mithramycin (200 nM) in minimal media. The cells were then stimulated with 100 ng/ml rhM-CSF (M-CSF) , (M-CSF+MeOH) , (M-CSF+Mith (200 nM)) and analyzed as in A. The data represents the mean+/−SEM of four individual donors.
    Dctp, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 99/100, based on 1620 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    PerkinElmer dgtp
    M-CSF regulates VEGF mRNA transcription through ERK and Sp1. A) Freshly isolated human monocytes were cultured overnight in 5% FBS and subsequently starved for 2 hours in minimal media. The cells were stimulated with 100 ng/ml rhM-CSF for 6 hours followed by isolation of RNA. cDNA was synthesized from total cellular RNA, standardized, and subjected to <t>SYBR</t> Green real-time <t>PCR</t> using primers specific for VEGF-A. The data represents the mean+/−SEM of six individual donors. B) Freshly isolated human monocytes were cultured overnight in 5% FBS and subsequently starved for 2 hrs followed by pre-treatment with either DMSO (vehicle control) or U0126 (10 µM) in minimal media for 30 minutes. The cells were then stimulated with 100 ng/ml rhM-CSF (M-CSF) , (M-CSF+DMSO) , (M-CSF+U0126 10 µM) and analyzed as stated in A. The data represents the mean+/−SEM of three individual donors. C) Freshly isolated human monocytes were cultured overnight in 5% FBS and subsequently starved for 2 hours followed by pre-treatment with either methanol (vehicle control) or mithramycin (200 nM) in minimal media. The cells were then stimulated with 100 ng/ml rhM-CSF (M-CSF) , (M-CSF+MeOH) , (M-CSF+Mith (200 nM)) and analyzed as in A. The data represents the mean+/−SEM of four individual donors.
    Dgtp, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 99/100, based on 1671 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    PerkinElmer datp
    M-CSF regulates VEGF mRNA transcription through ERK and Sp1. A) Freshly isolated human monocytes were cultured overnight in 5% FBS and subsequently starved for 2 hours in minimal media. The cells were stimulated with 100 ng/ml rhM-CSF for 6 hours followed by isolation of RNA. cDNA was synthesized from total cellular RNA, standardized, and subjected to <t>SYBR</t> Green real-time <t>PCR</t> using primers specific for VEGF-A. The data represents the mean+/−SEM of six individual donors. B) Freshly isolated human monocytes were cultured overnight in 5% FBS and subsequently starved for 2 hrs followed by pre-treatment with either DMSO (vehicle control) or U0126 (10 µM) in minimal media for 30 minutes. The cells were then stimulated with 100 ng/ml rhM-CSF (M-CSF) , (M-CSF+DMSO) , (M-CSF+U0126 10 µM) and analyzed as stated in A. The data represents the mean+/−SEM of three individual donors. C) Freshly isolated human monocytes were cultured overnight in 5% FBS and subsequently starved for 2 hours followed by pre-treatment with either methanol (vehicle control) or mithramycin (200 nM) in minimal media. The cells were then stimulated with 100 ng/ml rhM-CSF (M-CSF) , (M-CSF+MeOH) , (M-CSF+Mith (200 nM)) and analyzed as in A. The data represents the mean+/−SEM of four individual donors.
    Datp, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 99/100, based on 2767 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    PerkinElmer genomic dna
    Mutation detection of UNC93A in ovarian tumors. A) Mutation detection using P 32 -SSCP method. Each exon was amplified with DNAs from tumour sample, its matched control and normal placenta tissue. The variant bands in exon 3 and 4 in the tumour samples and their matched normals compared with normal placenta tissue are indicated by arrows. B) Mutation detection using F-SSCP method. Representative examples are shown for exons 3, 4, and 8. The primers were labelled with fluorescent dye (the forward primers were labelled with HEX, the reverse primers were labelled with FAM). The electrograms are a graphical display of the fluorescent intensity on the y-axis and the mobility along the x-axis. The red peaks are the GeneScan 500 size standard (Perkin Elmer) labelled with TAMRA, which functions as an internal control for each lane. Placenta <t>DNA</t> was used as a normal control. The abnormal peaks in the tumours are indicated by arrows. C) Mutation detection using DHPLC method. <t>PCR</t> was performed using primers flanking exons by 'touchdown' PCR and subjected to DHPLC analysis. The electrograms are a graphical display of the amount of DNA run through the column (intensity peak) on the y-axis and the retention time of the DNA in the column (minute) along the x-axis. The abnormal peaks in exon1 (T59) and exon 5 (T43) are indicated by arrows. PCR products from placenta DNA was used as control.
    Genomic Dna, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 99/100, based on 1090 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher taqman universal pcr master mix
    Mutation detection of UNC93A in ovarian tumors. A) Mutation detection using P 32 -SSCP method. Each exon was amplified with DNAs from tumour sample, its matched control and normal placenta tissue. The variant bands in exon 3 and 4 in the tumour samples and their matched normals compared with normal placenta tissue are indicated by arrows. B) Mutation detection using F-SSCP method. Representative examples are shown for exons 3, 4, and 8. The primers were labelled with fluorescent dye (the forward primers were labelled with HEX, the reverse primers were labelled with FAM). The electrograms are a graphical display of the fluorescent intensity on the y-axis and the mobility along the x-axis. The red peaks are the GeneScan 500 size standard (Perkin Elmer) labelled with TAMRA, which functions as an internal control for each lane. Placenta <t>DNA</t> was used as a normal control. The abnormal peaks in the tumours are indicated by arrows. C) Mutation detection using DHPLC method. <t>PCR</t> was performed using primers flanking exons by 'touchdown' PCR and subjected to DHPLC analysis. The electrograms are a graphical display of the amount of DNA run through the column (intensity peak) on the y-axis and the retention time of the DNA in the column (minute) along the x-axis. The abnormal peaks in exon1 (T59) and exon 5 (T43) are indicated by arrows. PCR products from placenta DNA was used as control.
    Taqman Universal Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 54513 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher mgcl2
    Mutation detection of UNC93A in ovarian tumors. A) Mutation detection using P 32 -SSCP method. Each exon was amplified with DNAs from tumour sample, its matched control and normal placenta tissue. The variant bands in exon 3 and 4 in the tumour samples and their matched normals compared with normal placenta tissue are indicated by arrows. B) Mutation detection using F-SSCP method. Representative examples are shown for exons 3, 4, and 8. The primers were labelled with fluorescent dye (the forward primers were labelled with HEX, the reverse primers were labelled with FAM). The electrograms are a graphical display of the fluorescent intensity on the y-axis and the mobility along the x-axis. The red peaks are the GeneScan 500 size standard (Perkin Elmer) labelled with TAMRA, which functions as an internal control for each lane. Placenta <t>DNA</t> was used as a normal control. The abnormal peaks in the tumours are indicated by arrows. C) Mutation detection using DHPLC method. <t>PCR</t> was performed using primers flanking exons by 'touchdown' PCR and subjected to DHPLC analysis. The electrograms are a graphical display of the amount of DNA run through the column (intensity peak) on the y-axis and the retention time of the DNA in the column (minute) along the x-axis. The abnormal peaks in exon1 (T59) and exon 5 (T43) are indicated by arrows. PCR products from placenta DNA was used as control.
    Mgcl2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 109347 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher amplitaq gold dna polymerase
    Mutation detection of UNC93A in ovarian tumors. A) Mutation detection using P 32 -SSCP method. Each exon was amplified with DNAs from tumour sample, its matched control and normal placenta tissue. The variant bands in exon 3 and 4 in the tumour samples and their matched normals compared with normal placenta tissue are indicated by arrows. B) Mutation detection using F-SSCP method. Representative examples are shown for exons 3, 4, and 8. The primers were labelled with fluorescent dye (the forward primers were labelled with HEX, the reverse primers were labelled with FAM). The electrograms are a graphical display of the fluorescent intensity on the y-axis and the mobility along the x-axis. The red peaks are the GeneScan 500 size standard (Perkin Elmer) labelled with TAMRA, which functions as an internal control for each lane. Placenta <t>DNA</t> was used as a normal control. The abnormal peaks in the tumours are indicated by arrows. C) Mutation detection using DHPLC method. <t>PCR</t> was performed using primers flanking exons by 'touchdown' PCR and subjected to DHPLC analysis. The electrograms are a graphical display of the amount of DNA run through the column (intensity peak) on the y-axis and the retention time of the DNA in the column (minute) along the x-axis. The abnormal peaks in exon1 (T59) and exon 5 (T43) are indicated by arrows. PCR products from placenta DNA was used as control.
    Amplitaq Gold Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 13081 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher applied biosystems dna sequencer
    Mutation detection of UNC93A in ovarian tumors. A) Mutation detection using P 32 -SSCP method. Each exon was amplified with DNAs from tumour sample, its matched control and normal placenta tissue. The variant bands in exon 3 and 4 in the tumour samples and their matched normals compared with normal placenta tissue are indicated by arrows. B) Mutation detection using F-SSCP method. Representative examples are shown for exons 3, 4, and 8. The primers were labelled with fluorescent dye (the forward primers were labelled with HEX, the reverse primers were labelled with FAM). The electrograms are a graphical display of the fluorescent intensity on the y-axis and the mobility along the x-axis. The red peaks are the GeneScan 500 size standard (Perkin Elmer) labelled with TAMRA, which functions as an internal control for each lane. Placenta <t>DNA</t> was used as a normal control. The abnormal peaks in the tumours are indicated by arrows. C) Mutation detection using DHPLC method. <t>PCR</t> was performed using primers flanking exons by 'touchdown' PCR and subjected to DHPLC analysis. The electrograms are a graphical display of the amount of DNA run through the column (intensity peak) on the y-axis and the retention time of the DNA in the column (minute) along the x-axis. The abnormal peaks in exon1 (T59) and exon 5 (T43) are indicated by arrows. PCR products from placenta DNA was used as control.
    Applied Biosystems Dna Sequencer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 714 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    PerkinElmer dttp
    Mutation detection of UNC93A in ovarian tumors. A) Mutation detection using P 32 -SSCP method. Each exon was amplified with DNAs from tumour sample, its matched control and normal placenta tissue. The variant bands in exon 3 and 4 in the tumour samples and their matched normals compared with normal placenta tissue are indicated by arrows. B) Mutation detection using F-SSCP method. Representative examples are shown for exons 3, 4, and 8. The primers were labelled with fluorescent dye (the forward primers were labelled with HEX, the reverse primers were labelled with FAM). The electrograms are a graphical display of the fluorescent intensity on the y-axis and the mobility along the x-axis. The red peaks are the GeneScan 500 size standard (Perkin Elmer) labelled with TAMRA, which functions as an internal control for each lane. Placenta <t>DNA</t> was used as a normal control. The abnormal peaks in the tumours are indicated by arrows. C) Mutation detection using DHPLC method. <t>PCR</t> was performed using primers flanking exons by 'touchdown' PCR and subjected to DHPLC analysis. The electrograms are a graphical display of the amount of DNA run through the column (intensity peak) on the y-axis and the retention time of the DNA in the column (minute) along the x-axis. The abnormal peaks in exon1 (T59) and exon 5 (T43) are indicated by arrows. PCR products from placenta DNA was used as control.
    Dttp, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 96/100, based on 255 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher power sybr green pcr master mix
    Mutation detection of UNC93A in ovarian tumors. A) Mutation detection using P 32 -SSCP method. Each exon was amplified with DNAs from tumour sample, its matched control and normal placenta tissue. The variant bands in exon 3 and 4 in the tumour samples and their matched normals compared with normal placenta tissue are indicated by arrows. B) Mutation detection using F-SSCP method. Representative examples are shown for exons 3, 4, and 8. The primers were labelled with fluorescent dye (the forward primers were labelled with HEX, the reverse primers were labelled with FAM). The electrograms are a graphical display of the fluorescent intensity on the y-axis and the mobility along the x-axis. The red peaks are the GeneScan 500 size standard (Perkin Elmer) labelled with TAMRA, which functions as an internal control for each lane. Placenta <t>DNA</t> was used as a normal control. The abnormal peaks in the tumours are indicated by arrows. C) Mutation detection using DHPLC method. <t>PCR</t> was performed using primers flanking exons by 'touchdown' PCR and subjected to DHPLC analysis. The electrograms are a graphical display of the amount of DNA run through the column (intensity peak) on the y-axis and the retention time of the DNA in the column (minute) along the x-axis. The abnormal peaks in exon1 (T59) and exon 5 (T43) are indicated by arrows. PCR products from placenta DNA was used as control.
    Power Sybr Green Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 69800 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybr green master mix
    Mutation detection of UNC93A in ovarian tumors. A) Mutation detection using P 32 -SSCP method. Each exon was amplified with DNAs from tumour sample, its matched control and normal placenta tissue. The variant bands in exon 3 and 4 in the tumour samples and their matched normals compared with normal placenta tissue are indicated by arrows. B) Mutation detection using F-SSCP method. Representative examples are shown for exons 3, 4, and 8. The primers were labelled with fluorescent dye (the forward primers were labelled with HEX, the reverse primers were labelled with FAM). The electrograms are a graphical display of the fluorescent intensity on the y-axis and the mobility along the x-axis. The red peaks are the GeneScan 500 size standard (Perkin Elmer) labelled with TAMRA, which functions as an internal control for each lane. Placenta <t>DNA</t> was used as a normal control. The abnormal peaks in the tumours are indicated by arrows. C) Mutation detection using DHPLC method. <t>PCR</t> was performed using primers flanking exons by 'touchdown' PCR and subjected to DHPLC analysis. The electrograms are a graphical display of the amount of DNA run through the column (intensity peak) on the y-axis and the retention time of the DNA in the column (minute) along the x-axis. The abnormal peaks in exon1 (T59) and exon 5 (T43) are indicated by arrows. PCR products from placenta DNA was used as control.
    Sybr Green Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 33941 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher power sybr green rna to ct 1 step kit
    Mutation detection of UNC93A in ovarian tumors. A) Mutation detection using P 32 -SSCP method. Each exon was amplified with DNAs from tumour sample, its matched control and normal placenta tissue. The variant bands in exon 3 and 4 in the tumour samples and their matched normals compared with normal placenta tissue are indicated by arrows. B) Mutation detection using F-SSCP method. Representative examples are shown for exons 3, 4, and 8. The primers were labelled with fluorescent dye (the forward primers were labelled with HEX, the reverse primers were labelled with FAM). The electrograms are a graphical display of the fluorescent intensity on the y-axis and the mobility along the x-axis. The red peaks are the GeneScan 500 size standard (Perkin Elmer) labelled with TAMRA, which functions as an internal control for each lane. Placenta <t>DNA</t> was used as a normal control. The abnormal peaks in the tumours are indicated by arrows. C) Mutation detection using DHPLC method. <t>PCR</t> was performed using primers flanking exons by 'touchdown' PCR and subjected to DHPLC analysis. The electrograms are a graphical display of the amount of DNA run through the column (intensity peak) on the y-axis and the retention time of the DNA in the column (minute) along the x-axis. The abnormal peaks in exon1 (T59) and exon 5 (T43) are indicated by arrows. PCR products from placenta DNA was used as control.
    Power Sybr Green Rna To Ct 1 Step Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2797 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher amplitaq dna polymerase
    Mutation detection of UNC93A in ovarian tumors. A) Mutation detection using P 32 -SSCP method. Each exon was amplified with DNAs from tumour sample, its matched control and normal placenta tissue. The variant bands in exon 3 and 4 in the tumour samples and their matched normals compared with normal placenta tissue are indicated by arrows. B) Mutation detection using F-SSCP method. Representative examples are shown for exons 3, 4, and 8. The primers were labelled with fluorescent dye (the forward primers were labelled with HEX, the reverse primers were labelled with FAM). The electrograms are a graphical display of the fluorescent intensity on the y-axis and the mobility along the x-axis. The red peaks are the GeneScan 500 size standard (Perkin Elmer) labelled with TAMRA, which functions as an internal control for each lane. Placenta <t>DNA</t> was used as a normal control. The abnormal peaks in the tumours are indicated by arrows. C) Mutation detection using DHPLC method. <t>PCR</t> was performed using primers flanking exons by 'touchdown' PCR and subjected to DHPLC analysis. The electrograms are a graphical display of the amount of DNA run through the column (intensity peak) on the y-axis and the retention time of the DNA in the column (minute) along the x-axis. The abnormal peaks in exon1 (T59) and exon 5 (T43) are indicated by arrows. PCR products from placenta DNA was used as control.
    Amplitaq Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5408 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher pcr buffer
    Mutation detection of UNC93A in ovarian tumors. A) Mutation detection using P 32 -SSCP method. Each exon was amplified with DNAs from tumour sample, its matched control and normal placenta tissue. The variant bands in exon 3 and 4 in the tumour samples and their matched normals compared with normal placenta tissue are indicated by arrows. B) Mutation detection using F-SSCP method. Representative examples are shown for exons 3, 4, and 8. The primers were labelled with fluorescent dye (the forward primers were labelled with HEX, the reverse primers were labelled with FAM). The electrograms are a graphical display of the fluorescent intensity on the y-axis and the mobility along the x-axis. The red peaks are the GeneScan 500 size standard (Perkin Elmer) labelled with TAMRA, which functions as an internal control for each lane. Placenta <t>DNA</t> was used as a normal control. The abnormal peaks in the tumours are indicated by arrows. C) Mutation detection using DHPLC method. <t>PCR</t> was performed using primers flanking exons by 'touchdown' PCR and subjected to DHPLC analysis. The electrograms are a graphical display of the amount of DNA run through the column (intensity peak) on the y-axis and the retention time of the DNA in the column (minute) along the x-axis. The abnormal peaks in exon1 (T59) and exon 5 (T43) are indicated by arrows. PCR products from placenta DNA was used as control.
    Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 28828 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher abi3100 sequence analyser
    Mutation detection of UNC93A in ovarian tumors. A) Mutation detection using P 32 -SSCP method. Each exon was amplified with DNAs from tumour sample, its matched control and normal placenta tissue. The variant bands in exon 3 and 4 in the tumour samples and their matched normals compared with normal placenta tissue are indicated by arrows. B) Mutation detection using F-SSCP method. Representative examples are shown for exons 3, 4, and 8. The primers were labelled with fluorescent dye (the forward primers were labelled with HEX, the reverse primers were labelled with FAM). The electrograms are a graphical display of the fluorescent intensity on the y-axis and the mobility along the x-axis. The red peaks are the GeneScan 500 size standard (Perkin Elmer) labelled with TAMRA, which functions as an internal control for each lane. Placenta <t>DNA</t> was used as a normal control. The abnormal peaks in the tumours are indicated by arrows. C) Mutation detection using DHPLC method. <t>PCR</t> was performed using primers flanking exons by 'touchdown' PCR and subjected to DHPLC analysis. The electrograms are a graphical display of the amount of DNA run through the column (intensity peak) on the y-axis and the retention time of the DNA in the column (minute) along the x-axis. The abnormal peaks in exon1 (T59) and exon 5 (T43) are indicated by arrows. PCR products from placenta DNA was used as control.
    Abi3100 Sequence Analyser, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    abi3100 sequence analyser - by Bioz Stars, 2020-09
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    99
    PerkinElmer amplitaq gold dna polymerase
    Mutation detection of UNC93A in ovarian tumors. A) Mutation detection using P 32 -SSCP method. Each exon was amplified with DNAs from tumour sample, its matched control and normal placenta tissue. The variant bands in exon 3 and 4 in the tumour samples and their matched normals compared with normal placenta tissue are indicated by arrows. B) Mutation detection using F-SSCP method. Representative examples are shown for exons 3, 4, and 8. The primers were labelled with fluorescent dye (the forward primers were labelled with HEX, the reverse primers were labelled with FAM). The electrograms are a graphical display of the fluorescent intensity on the y-axis and the mobility along the x-axis. The red peaks are the GeneScan 500 size standard (Perkin Elmer) labelled with TAMRA, which functions as an internal control for each lane. Placenta <t>DNA</t> was used as a normal control. The abnormal peaks in the tumours are indicated by arrows. C) Mutation detection using DHPLC method. <t>PCR</t> was performed using primers flanking exons by 'touchdown' PCR and subjected to DHPLC analysis. The electrograms are a graphical display of the amount of DNA run through the column (intensity peak) on the y-axis and the retention time of the DNA in the column (minute) along the x-axis. The abnormal peaks in exon1 (T59) and exon 5 (T43) are indicated by arrows. PCR products from placenta DNA was used as control.
    Amplitaq Gold Dna Polymerase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 99/100, based on 1608 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher moloney murine leukemia virus reverse transcriptase
    Mutation detection of UNC93A in ovarian tumors. A) Mutation detection using P 32 -SSCP method. Each exon was amplified with DNAs from tumour sample, its matched control and normal placenta tissue. The variant bands in exon 3 and 4 in the tumour samples and their matched normals compared with normal placenta tissue are indicated by arrows. B) Mutation detection using F-SSCP method. Representative examples are shown for exons 3, 4, and 8. The primers were labelled with fluorescent dye (the forward primers were labelled with HEX, the reverse primers were labelled with FAM). The electrograms are a graphical display of the fluorescent intensity on the y-axis and the mobility along the x-axis. The red peaks are the GeneScan 500 size standard (Perkin Elmer) labelled with TAMRA, which functions as an internal control for each lane. Placenta <t>DNA</t> was used as a normal control. The abnormal peaks in the tumours are indicated by arrows. C) Mutation detection using DHPLC method. <t>PCR</t> was performed using primers flanking exons by 'touchdown' PCR and subjected to DHPLC analysis. The electrograms are a graphical display of the amount of DNA run through the column (intensity peak) on the y-axis and the retention time of the DNA in the column (minute) along the x-axis. The abnormal peaks in exon1 (T59) and exon 5 (T43) are indicated by arrows. PCR products from placenta DNA was used as control.
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    M-CSF regulates VEGF mRNA transcription through ERK and Sp1. A) Freshly isolated human monocytes were cultured overnight in 5% FBS and subsequently starved for 2 hours in minimal media. The cells were stimulated with 100 ng/ml rhM-CSF for 6 hours followed by isolation of RNA. cDNA was synthesized from total cellular RNA, standardized, and subjected to SYBR Green real-time PCR using primers specific for VEGF-A. The data represents the mean+/−SEM of six individual donors. B) Freshly isolated human monocytes were cultured overnight in 5% FBS and subsequently starved for 2 hrs followed by pre-treatment with either DMSO (vehicle control) or U0126 (10 µM) in minimal media for 30 minutes. The cells were then stimulated with 100 ng/ml rhM-CSF (M-CSF) , (M-CSF+DMSO) , (M-CSF+U0126 10 µM) and analyzed as stated in A. The data represents the mean+/−SEM of three individual donors. C) Freshly isolated human monocytes were cultured overnight in 5% FBS and subsequently starved for 2 hours followed by pre-treatment with either methanol (vehicle control) or mithramycin (200 nM) in minimal media. The cells were then stimulated with 100 ng/ml rhM-CSF (M-CSF) , (M-CSF+MeOH) , (M-CSF+Mith (200 nM)) and analyzed as in A. The data represents the mean+/−SEM of four individual donors.

    Journal: PLoS ONE

    Article Title: M-CSF Signals through the MAPK/ERK Pathway via Sp1 to Induce VEGF Production and Induces Angiogenesis In Vivo

    doi: 10.1371/journal.pone.0003405

    Figure Lengend Snippet: M-CSF regulates VEGF mRNA transcription through ERK and Sp1. A) Freshly isolated human monocytes were cultured overnight in 5% FBS and subsequently starved for 2 hours in minimal media. The cells were stimulated with 100 ng/ml rhM-CSF for 6 hours followed by isolation of RNA. cDNA was synthesized from total cellular RNA, standardized, and subjected to SYBR Green real-time PCR using primers specific for VEGF-A. The data represents the mean+/−SEM of six individual donors. B) Freshly isolated human monocytes were cultured overnight in 5% FBS and subsequently starved for 2 hrs followed by pre-treatment with either DMSO (vehicle control) or U0126 (10 µM) in minimal media for 30 minutes. The cells were then stimulated with 100 ng/ml rhM-CSF (M-CSF) , (M-CSF+DMSO) , (M-CSF+U0126 10 µM) and analyzed as stated in A. The data represents the mean+/−SEM of three individual donors. C) Freshly isolated human monocytes were cultured overnight in 5% FBS and subsequently starved for 2 hours followed by pre-treatment with either methanol (vehicle control) or mithramycin (200 nM) in minimal media. The cells were then stimulated with 100 ng/ml rhM-CSF (M-CSF) , (M-CSF+MeOH) , (M-CSF+Mith (200 nM)) and analyzed as in A. The data represents the mean+/−SEM of four individual donors.

    Article Snippet: Reaction conditions were as follows: 10 µl SYBR® Green PCR Master Mix (Applied Biosystems), 0.4 µl each forward and reverse VEGF primers (10 µM) or primers targeting GAPDH (control), 8.2 µl RNase-free water, and 1.0 µl cDNA (0.5 µg) for a total reaction of 20 µl.

    Techniques: Isolation, Cell Culture, Synthesized, SYBR Green Assay, Real-time Polymerase Chain Reaction

    Mutation detection of UNC93A in ovarian tumors. A) Mutation detection using P 32 -SSCP method. Each exon was amplified with DNAs from tumour sample, its matched control and normal placenta tissue. The variant bands in exon 3 and 4 in the tumour samples and their matched normals compared with normal placenta tissue are indicated by arrows. B) Mutation detection using F-SSCP method. Representative examples are shown for exons 3, 4, and 8. The primers were labelled with fluorescent dye (the forward primers were labelled with HEX, the reverse primers were labelled with FAM). The electrograms are a graphical display of the fluorescent intensity on the y-axis and the mobility along the x-axis. The red peaks are the GeneScan 500 size standard (Perkin Elmer) labelled with TAMRA, which functions as an internal control for each lane. Placenta DNA was used as a normal control. The abnormal peaks in the tumours are indicated by arrows. C) Mutation detection using DHPLC method. PCR was performed using primers flanking exons by 'touchdown' PCR and subjected to DHPLC analysis. The electrograms are a graphical display of the amount of DNA run through the column (intensity peak) on the y-axis and the retention time of the DNA in the column (minute) along the x-axis. The abnormal peaks in exon1 (T59) and exon 5 (T43) are indicated by arrows. PCR products from placenta DNA was used as control.

    Journal: BMC Genetics

    Article Title: The human homologue of unc-93 maps to chromosome 6q27 - characterisation and analysis in sporadic epithelial ovarian cancer

    doi: 10.1186/1471-2156-3-20

    Figure Lengend Snippet: Mutation detection of UNC93A in ovarian tumors. A) Mutation detection using P 32 -SSCP method. Each exon was amplified with DNAs from tumour sample, its matched control and normal placenta tissue. The variant bands in exon 3 and 4 in the tumour samples and their matched normals compared with normal placenta tissue are indicated by arrows. B) Mutation detection using F-SSCP method. Representative examples are shown for exons 3, 4, and 8. The primers were labelled with fluorescent dye (the forward primers were labelled with HEX, the reverse primers were labelled with FAM). The electrograms are a graphical display of the fluorescent intensity on the y-axis and the mobility along the x-axis. The red peaks are the GeneScan 500 size standard (Perkin Elmer) labelled with TAMRA, which functions as an internal control for each lane. Placenta DNA was used as a normal control. The abnormal peaks in the tumours are indicated by arrows. C) Mutation detection using DHPLC method. PCR was performed using primers flanking exons by 'touchdown' PCR and subjected to DHPLC analysis. The electrograms are a graphical display of the amount of DNA run through the column (intensity peak) on the y-axis and the retention time of the DNA in the column (minute) along the x-axis. The abnormal peaks in exon1 (T59) and exon 5 (T43) are indicated by arrows. PCR products from placenta DNA was used as control.

    Article Snippet: Briefly, a 50 μl PCR reaction contained 30 ng genomic DNA, 10 pmol of each primer, 0.5 mM of dNTPs, 0.3 μl of a combination of Amplitaq Gold (Perkin Elmer) and Pfu Turbo (Stratagene) at 9:1 ratio, 5 μl 10 × AmpliTaq Gold buffer (Perkin Elmer), and 3 to 5 μl of MgCl2 (25 mM) optimised for each primer set (Table ).

    Techniques: Mutagenesis, Amplification, Variant Assay, Polymerase Chain Reaction, Touchdown PCR

    c.676C > T mutation in normal DNA . Agarose gel showing restriction digest by Nde1 of exon 5 amplified by PCR from normal DNA samples. The PCR product from ORK 29 shows an additional band of appropriate size suggesting heterozygous alteration, c.676C > T.

    Journal: BMC Genetics

    Article Title: The human homologue of unc-93 maps to chromosome 6q27 - characterisation and analysis in sporadic epithelial ovarian cancer

    doi: 10.1186/1471-2156-3-20

    Figure Lengend Snippet: c.676C > T mutation in normal DNA . Agarose gel showing restriction digest by Nde1 of exon 5 amplified by PCR from normal DNA samples. The PCR product from ORK 29 shows an additional band of appropriate size suggesting heterozygous alteration, c.676C > T.

    Article Snippet: Briefly, a 50 μl PCR reaction contained 30 ng genomic DNA, 10 pmol of each primer, 0.5 mM of dNTPs, 0.3 μl of a combination of Amplitaq Gold (Perkin Elmer) and Pfu Turbo (Stratagene) at 9:1 ratio, 5 μl 10 × AmpliTaq Gold buffer (Perkin Elmer), and 3 to 5 μl of MgCl2 (25 mM) optimised for each primer set (Table ).

    Techniques: Mutagenesis, Agarose Gel Electrophoresis, Amplification, Polymerase Chain Reaction