amplitaq dna polymerase Perkinelmer Search Results


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  • 78
    PerkinElmer amplitac dna polymerase
    Amplitac Dna Polymerase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 78 stars, based on 4 article reviews
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    78/100 stars
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    99
    PerkinElmer amplitaq dna polymerase
    Comparison of the 16S-rRNA-nested-PCR assay with primer pairs ge3a-ge10 and ge9-ge2 with commercially available kits from three manufacturers: (A) PerkinElmer GeneAmp PCR reagent kit with <t>AmpliTaq</t> <t>DNA</t> polymerase; (B) Amersham Pharmacia Ready-To-Go PCR beads; (C) Qiagen Taq PCR Master Mix kit. Lanes 1 through 5 represent amplifications from a 10-fold dilution series ranging from 5 × 10 5 A. phagocytophilum -infected cells/ml to 50 infected cells/ml, and lane 6 contains DNA from uninfected human blood as a negative control. Phage ϕX174 DNA digested with Hae III was run in the lanes labeled M. The arrows indicate the position of the 546-bp amplified products.
    Amplitaq Dna Polymerase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 99/100, based on 3756 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    PerkinElmer amplitag dna polymerase
    Comparison of the 16S-rRNA-nested-PCR assay with primer pairs ge3a-ge10 and ge9-ge2 with commercially available kits from three manufacturers: (A) PerkinElmer GeneAmp PCR reagent kit with <t>AmpliTaq</t> <t>DNA</t> polymerase; (B) Amersham Pharmacia Ready-To-Go PCR beads; (C) Qiagen Taq PCR Master Mix kit. Lanes 1 through 5 represent amplifications from a 10-fold dilution series ranging from 5 × 10 5 A. phagocytophilum -infected cells/ml to 50 infected cells/ml, and lane 6 contains DNA from uninfected human blood as a negative control. Phage ϕX174 DNA digested with Hae III was run in the lanes labeled M. The arrows indicate the position of the 546-bp amplified products.
    Amplitag Dna Polymerase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 77/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    PerkinElmer amplitaq thermostable dna polymerase
    Comparison of the 16S-rRNA-nested-PCR assay with primer pairs ge3a-ge10 and ge9-ge2 with commercially available kits from three manufacturers: (A) PerkinElmer GeneAmp PCR reagent kit with <t>AmpliTaq</t> <t>DNA</t> polymerase; (B) Amersham Pharmacia Ready-To-Go PCR beads; (C) Qiagen Taq PCR Master Mix kit. Lanes 1 through 5 represent amplifications from a 10-fold dilution series ranging from 5 × 10 5 A. phagocytophilum -infected cells/ml to 50 infected cells/ml, and lane 6 contains DNA from uninfected human blood as a negative control. Phage ϕX174 DNA digested with Hae III was run in the lanes labeled M. The arrows indicate the position of the 546-bp amplified products.
    Amplitaq Thermostable Dna Polymerase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 79/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    PerkinElmer amplitaq gold dna polymerase
    Amplification of eight copies of HPV-16 accomplished in the presence of TE buffer (lanes b, c, and d), 0.04% (final concentration [vol/vol]) NP-40 and 0.04% Tween (lanes e and f), 0.08% Tween 20 (lanes g and h), and 0.08% NP-40 (lanes i and j). The negative control is in lane a. Amplification was done with <t>AmpliTaq</t> Gold <t>DNA</t> polymerase in a model 9600 DNA thermal cycler. A 450-bp band is visible in positive reactions.
    Amplitaq Gold Dna Polymerase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 94/100, based on 1426 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    PerkinElmer amplitaq goldr dna polymerase
    Amplification of eight copies of HPV-16 accomplished in the presence of TE buffer (lanes b, c, and d), 0.04% (final concentration [vol/vol]) NP-40 and 0.04% Tween (lanes e and f), 0.08% Tween 20 (lanes g and h), and 0.08% NP-40 (lanes i and j). The negative control is in lane a. Amplification was done with <t>AmpliTaq</t> Gold <t>DNA</t> polymerase in a model 9600 DNA thermal cycler. A 450-bp band is visible in positive reactions.
    Amplitaq Goldr Dna Polymerase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 89/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    PerkinElmer u amplitaqr dna polymerase
    Amplification of eight copies of HPV-16 accomplished in the presence of TE buffer (lanes b, c, and d), 0.04% (final concentration [vol/vol]) NP-40 and 0.04% Tween (lanes e and f), 0.08% Tween 20 (lanes g and h), and 0.08% NP-40 (lanes i and j). The negative control is in lane a. Amplification was done with <t>AmpliTaq</t> Gold <t>DNA</t> polymerase in a model 9600 DNA thermal cycler. A 450-bp band is visible in positive reactions.
    U Amplitaqr Dna Polymerase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 78/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    PerkinElmer dna polymerase
    <t>SP-C/p40</t> <t>DNA</t> construct. The construct contains a 3·7 kb fragment from the SP-C promoter, a 1 kb murine IL-12p40 cDNA fragment, an intron and SV 40 polyadenylation sequences. Restriction sites and primers used in the PCR are indicated.
    Dna Polymerase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 90/100, based on 165 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    PerkinElmer enzyme amplitaq dna polymerase fs
    <t>SP-C/p40</t> <t>DNA</t> construct. The construct contains a 3·7 kb fragment from the SP-C promoter, a 1 kb murine IL-12p40 cDNA fragment, an intron and SV 40 polyadenylation sequences. Restriction sites and primers used in the PCR are indicated.
    Enzyme Amplitaq Dna Polymerase Fs, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 78/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer amplitaq dna polymerase stoeffel fragment
    <t>SP-C/p40</t> <t>DNA</t> construct. The construct contains a 3·7 kb fragment from the SP-C promoter, a 1 kb murine IL-12p40 cDNA fragment, an intron and SV 40 polyadenylation sequences. Restriction sites and primers used in the PCR are indicated.
    Amplitaq Dna Polymerase Stoeffel Fragment, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer sequencing amplitaq dna polymerase
    <t>SP-C/p40</t> <t>DNA</t> construct. The construct contains a 3·7 kb fragment from the SP-C promoter, a 1 kb murine IL-12p40 cDNA fragment, an intron and SV 40 polyadenylation sequences. Restriction sites and primers used in the PCR are indicated.
    Sequencing Amplitaq Dna Polymerase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    PerkinElmer amplitaq dna polymerase stoffel fragment
    <t>SP-C/p40</t> <t>DNA</t> construct. The construct contains a 3·7 kb fragment from the SP-C promoter, a 1 kb murine IL-12p40 cDNA fragment, an intron and SV 40 polyadenylation sequences. Restriction sites and primers used in the PCR are indicated.
    Amplitaq Dna Polymerase Stoffel Fragment, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 78/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer amplitaq gold dna polymerase kit
    <t>SP-C/p40</t> <t>DNA</t> construct. The construct contains a 3·7 kb fragment from the SP-C promoter, a 1 kb murine IL-12p40 cDNA fragment, an intron and SV 40 polyadenylation sequences. Restriction sites and primers used in the PCR are indicated.
    Amplitaq Gold Dna Polymerase Kit, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 78/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    PerkinElmer thermostable dna polymerase
    <t>SP-C/p40</t> <t>DNA</t> construct. The construct contains a 3·7 kb fragment from the SP-C promoter, a 1 kb murine IL-12p40 cDNA fragment, an intron and SV 40 polyadenylation sequences. Restriction sites and primers used in the PCR are indicated.
    Thermostable Dna Polymerase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer multiplex pcrs amplitaq gold dna polymerase
    <t>SP-C/p40</t> <t>DNA</t> construct. The construct contains a 3·7 kb fragment from the SP-C promoter, a 1 kb murine IL-12p40 cDNA fragment, an intron and SV 40 polyadenylation sequences. Restriction sites and primers used in the PCR are indicated.
    Multiplex Pcrs Amplitaq Gold Dna Polymerase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer amplitaq dna polymerase fs dna sequencing kit
    <t>SP-C/p40</t> <t>DNA</t> construct. The construct contains a 3·7 kb fragment from the SP-C promoter, a 1 kb murine IL-12p40 cDNA fragment, an intron and SV 40 polyadenylation sequences. Restriction sites and primers used in the PCR are indicated.
    Amplitaq Dna Polymerase Fs Dna Sequencing Kit, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 78/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer amplitaq fstm dna polymerase
    <t>SP-C/p40</t> <t>DNA</t> construct. The construct contains a 3·7 kb fragment from the SP-C promoter, a 1 kb murine IL-12p40 cDNA fragment, an intron and SV 40 polyadenylation sequences. Restriction sites and primers used in the PCR are indicated.
    Amplitaq Fstm Dna Polymerase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer amplitaq dna polymerase protocol
    <t>SP-C/p40</t> <t>DNA</t> construct. The construct contains a 3·7 kb fragment from the SP-C promoter, a 1 kb murine IL-12p40 cDNA fragment, an intron and SV 40 polyadenylation sequences. Restriction sites and primers used in the PCR are indicated.
    Amplitaq Dna Polymerase Protocol, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 79/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer amplitaq dna polymerase kit
    <t>SP-C/p40</t> <t>DNA</t> construct. The construct contains a 3·7 kb fragment from the SP-C promoter, a 1 kb murine IL-12p40 cDNA fragment, an intron and SV 40 polyadenylation sequences. Restriction sites and primers used in the PCR are indicated.
    Amplitaq Dna Polymerase Kit, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 77/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer taq gold dna polymerase
    <t>SP-C/p40</t> <t>DNA</t> construct. The construct contains a 3·7 kb fragment from the SP-C promoter, a 1 kb murine IL-12p40 cDNA fragment, an intron and SV 40 polyadenylation sequences. Restriction sites and primers used in the PCR are indicated.
    Taq Gold Dna Polymerase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 88/100, based on 137 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    PerkinElmer amplitaq gold dna polymerase enzyme
    <t>SP-C/p40</t> <t>DNA</t> construct. The construct contains a 3·7 kb fragment from the SP-C promoter, a 1 kb murine IL-12p40 cDNA fragment, an intron and SV 40 polyadenylation sequences. Restriction sites and primers used in the PCR are indicated.
    Amplitaq Gold Dna Polymerase Enzyme, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 78/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    PerkinElmer amplitaq dna polymerase dye terminator cycle sequencing chemistry
    <t>SP-C/p40</t> <t>DNA</t> construct. The construct contains a 3·7 kb fragment from the SP-C promoter, a 1 kb murine IL-12p40 cDNA fragment, an intron and SV 40 polyadenylation sequences. Restriction sites and primers used in the PCR are indicated.
    Amplitaq Dna Polymerase Dye Terminator Cycle Sequencing Chemistry, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 79/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    PerkinElmer recombinant clones amplitaq dna polymerase
    <t>SP-C/p40</t> <t>DNA</t> construct. The construct contains a 3·7 kb fragment from the SP-C promoter, a 1 kb murine IL-12p40 cDNA fragment, an intron and SV 40 polyadenylation sequences. Restriction sites and primers used in the PCR are indicated.
    Recombinant Clones Amplitaq Dna Polymerase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 79/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    PerkinElmer taq dna polymerase
    <t>SP-C/p40</t> <t>DNA</t> construct. The construct contains a 3·7 kb fragment from the SP-C promoter, a 1 kb murine IL-12p40 cDNA fragment, an intron and SV 40 polyadenylation sequences. Restriction sites and primers used in the PCR are indicated.
    Taq Dna Polymerase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 93/100, based on 3390 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer prism amplitaq dna polymerase fs ready reaction dye terminator sequencing kit
    <t>SP-C/p40</t> <t>DNA</t> construct. The construct contains a 3·7 kb fragment from the SP-C promoter, a 1 kb murine IL-12p40 cDNA fragment, an intron and SV 40 polyadenylation sequences. Restriction sites and primers used in the PCR are indicated.
    Prism Amplitaq Dna Polymerase Fs Ready Reaction Dye Terminator Sequencing Kit, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 75/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    PerkinElmer thermus aquaticus dna polymerase
    <t>SP-C/p40</t> <t>DNA</t> construct. The construct contains a 3·7 kb fragment from the SP-C promoter, a 1 kb murine IL-12p40 cDNA fragment, an intron and SV 40 polyadenylation sequences. Restriction sites and primers used in the PCR are indicated.
    Thermus Aquaticus Dna Polymerase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 85/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer amplitaq gold dna polymerse
    <t>SP-C/p40</t> <t>DNA</t> construct. The construct contains a 3·7 kb fragment from the SP-C promoter, a 1 kb murine IL-12p40 cDNA fragment, an intron and SV 40 polyadenylation sequences. Restriction sites and primers used in the PCR are indicated.
    Amplitaq Gold Dna Polymerse, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 80/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    PerkinElmer taq dna polymerase mediated pcr amplification
    <t>SP-C/p40</t> <t>DNA</t> construct. The construct contains a 3·7 kb fragment from the SP-C promoter, a 1 kb murine IL-12p40 cDNA fragment, an intron and SV 40 polyadenylation sequences. Restriction sites and primers used in the PCR are indicated.
    Taq Dna Polymerase Mediated Pcr Amplification, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 78/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer amplitaq
    <t>SP-C/p40</t> <t>DNA</t> construct. The construct contains a 3·7 kb fragment from the SP-C promoter, a 1 kb murine IL-12p40 cDNA fragment, an intron and SV 40 polyadenylation sequences. Restriction sites and primers used in the PCR are indicated.
    Amplitaq, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 1136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer amplitaq ld
    <t>SP-C/p40</t> <t>DNA</t> construct. The construct contains a 3·7 kb fragment from the SP-C promoter, a 1 kb murine IL-12p40 cDNA fragment, an intron and SV 40 polyadenylation sequences. Restriction sites and primers used in the PCR are indicated.
    Amplitaq Ld, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 78/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    PerkinElmer pcr core reagents kit
    <t>SP-C/p40</t> <t>DNA</t> construct. The construct contains a 3·7 kb fragment from the SP-C promoter, a 1 kb murine IL-12p40 cDNA fragment, an intron and SV 40 polyadenylation sequences. Restriction sites and primers used in the PCR are indicated.
    Pcr Core Reagents Kit, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 86/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    PerkinElmer abi prism cycle sequencing kit
    <t>SP-C/p40</t> <t>DNA</t> construct. The construct contains a 3·7 kb fragment from the SP-C promoter, a 1 kb murine IL-12p40 cDNA fragment, an intron and SV 40 polyadenylation sequences. Restriction sites and primers used in the PCR are indicated.
    Abi Prism Cycle Sequencing Kit, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 85/100, based on 166 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Comparison of the 16S-rRNA-nested-PCR assay with primer pairs ge3a-ge10 and ge9-ge2 with commercially available kits from three manufacturers: (A) PerkinElmer GeneAmp PCR reagent kit with AmpliTaq DNA polymerase; (B) Amersham Pharmacia Ready-To-Go PCR beads; (C) Qiagen Taq PCR Master Mix kit. Lanes 1 through 5 represent amplifications from a 10-fold dilution series ranging from 5 × 10 5 A. phagocytophilum -infected cells/ml to 50 infected cells/ml, and lane 6 contains DNA from uninfected human blood as a negative control. Phage ϕX174 DNA digested with Hae III was run in the lanes labeled M. The arrows indicate the position of the 546-bp amplified products.

    Journal: Journal of Clinical Microbiology

    Article Title: Comparison of PCR Assays for Detection of the Agent of Human Granulocytic Ehrlichiosis, Anaplasma phagocytophilum

    doi: 10.1128/JCM.41.2.717-722.2003

    Figure Lengend Snippet: Comparison of the 16S-rRNA-nested-PCR assay with primer pairs ge3a-ge10 and ge9-ge2 with commercially available kits from three manufacturers: (A) PerkinElmer GeneAmp PCR reagent kit with AmpliTaq DNA polymerase; (B) Amersham Pharmacia Ready-To-Go PCR beads; (C) Qiagen Taq PCR Master Mix kit. Lanes 1 through 5 represent amplifications from a 10-fold dilution series ranging from 5 × 10 5 A. phagocytophilum -infected cells/ml to 50 infected cells/ml, and lane 6 contains DNA from uninfected human blood as a negative control. Phage ϕX174 DNA digested with Hae III was run in the lanes labeled M. The arrows indicate the position of the 546-bp amplified products.

    Article Snippet: The initial limit of detection testing used reagents from the GeneAmp PCR kit with AmpliTaq DNA polymerase (PerkinElmer, Foster City, Calif.).

    Techniques: Nested PCR, Polymerase Chain Reaction, Infection, Negative Control, Labeling, Amplification

    Amplification of eight copies of HPV-16 accomplished in the presence of TE buffer (lanes b, c, and d), 0.04% (final concentration [vol/vol]) NP-40 and 0.04% Tween (lanes e and f), 0.08% Tween 20 (lanes g and h), and 0.08% NP-40 (lanes i and j). The negative control is in lane a. Amplification was done with AmpliTaq Gold DNA polymerase in a model 9600 DNA thermal cycler. A 450-bp band is visible in positive reactions.

    Journal: Journal of Clinical Microbiology

    Article Title: Effect of Nonionic Detergents on Amplification of Human Papillomavirus DNA with Consensus Primers MY09 and MY11

    doi:

    Figure Lengend Snippet: Amplification of eight copies of HPV-16 accomplished in the presence of TE buffer (lanes b, c, and d), 0.04% (final concentration [vol/vol]) NP-40 and 0.04% Tween (lanes e and f), 0.08% Tween 20 (lanes g and h), and 0.08% NP-40 (lanes i and j). The negative control is in lane a. Amplification was done with AmpliTaq Gold DNA polymerase in a model 9600 DNA thermal cycler. A 450-bp band is visible in positive reactions.

    Article Snippet: Ten-microliter volumes of the detergent preparations described below were added to 90-μl volumes of a PCR master mix containing the usual components ( , ) plus eight copies of HPV-16 (kindly provided by H. zur Hausen), 5 U of AmpliTaq Gold DNA polymerase (Perkin-Elmer, Montréal, Canada), and the L1 consensus HPV primers MY09 and MY11.

    Techniques: Amplification, Concentration Assay, Negative Control

    SP-C/p40 DNA construct. The construct contains a 3·7 kb fragment from the SP-C promoter, a 1 kb murine IL-12p40 cDNA fragment, an intron and SV 40 polyadenylation sequences. Restriction sites and primers used in the PCR are indicated.

    Journal: Immunology

    Article Title: Mice overexpressing p40 in lungs have reduced leucocyte influx and slightly impaired resistance during tuberculosis

    doi: 10.1111/j.1365-2567.2005.02315.x

    Figure Lengend Snippet: SP-C/p40 DNA construct. The construct contains a 3·7 kb fragment from the SP-C promoter, a 1 kb murine IL-12p40 cDNA fragment, an intron and SV 40 polyadenylation sequences. Restriction sites and primers used in the PCR are indicated.

    Article Snippet: PCR was performed with 100 pg genomic DNA in a reaction mixture of 1·0 m m deoxynucleotide triphosphates (dNTPs), 10 mg/ml bovine serum albumin, 2% dimethyl sulphoxide, 10× Pol buffer (0·67 m Tris-HCl, pH 8·8; 67 m m MgCl2 ; 0,1 m β-mercaptoethanol; 67 µ m EDTA; 0·166 m (NH4 )2 SO4 ), 0·5 µ m of the SP-C-primer (5′-GGACACATATAAGACCC-TGG-3′), 0·5 µ m of the p40 primer (5′-TTTGGTGCTTCACACTTCAGG-3′) and 5 U/µl DNA polymerase (AmpliTaq, Perkin Elmer, Norwalk, CT).

    Techniques: Construct, Polymerase Chain Reaction

    A representative Southern blot (a) and PCR analysis (b) of the screening of transgenic mice.(a) Southern blot analysis of the genomic DNA isolated from mouse tails of the first generation mice after injection of the construct. On this blot four mice carry the transgene. (b) PCR analysis was performed on the fifth generation of transgenic mice. A PCR product of 800 bp because of the appearance of the p40 transgene in combination with the SP-C promoter was detected in all five mice tested. M = marker.

    Journal: Immunology

    Article Title: Mice overexpressing p40 in lungs have reduced leucocyte influx and slightly impaired resistance during tuberculosis

    doi: 10.1111/j.1365-2567.2005.02315.x

    Figure Lengend Snippet: A representative Southern blot (a) and PCR analysis (b) of the screening of transgenic mice.(a) Southern blot analysis of the genomic DNA isolated from mouse tails of the first generation mice after injection of the construct. On this blot four mice carry the transgene. (b) PCR analysis was performed on the fifth generation of transgenic mice. A PCR product of 800 bp because of the appearance of the p40 transgene in combination with the SP-C promoter was detected in all five mice tested. M = marker.

    Article Snippet: PCR was performed with 100 pg genomic DNA in a reaction mixture of 1·0 m m deoxynucleotide triphosphates (dNTPs), 10 mg/ml bovine serum albumin, 2% dimethyl sulphoxide, 10× Pol buffer (0·67 m Tris-HCl, pH 8·8; 67 m m MgCl2 ; 0,1 m β-mercaptoethanol; 67 µ m EDTA; 0·166 m (NH4 )2 SO4 ), 0·5 µ m of the SP-C-primer (5′-GGACACATATAAGACCC-TGG-3′), 0·5 µ m of the p40 primer (5′-TTTGGTGCTTCACACTTCAGG-3′) and 5 U/µl DNA polymerase (AmpliTaq, Perkin Elmer, Norwalk, CT).

    Techniques: Southern Blot, Polymerase Chain Reaction, Transgenic Assay, Mouse Assay, Isolation, Injection, Construct, Marker