amplitaq dna polymerase Search Results


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  • 99
    Thermo Fisher amplitaq high fidelity dna polymerase
    Confronting two-pair primers–polymerase chain reaction for NRF2 -617C/A and -653A/G was carried out at the indicated T a using 2.5U/sample of <t>AmpliTaq</t> Gold polymerase. N: Negative control indicates <t>DNA</t> template omission. MW: Molecular weights (100 bp up to 1000 bp).
    Amplitaq High Fidelity Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Thermo Fisher amplitaq 360 dna polymerase
    Inhibition and the effect of hot-start. ( a ) Inhibition profiles of 2D9 versus <t>AmpliTaq</t> <t>Gold</t> 360 (ATaqG) in PCR. The effect of various inhibitors (SP61: bone dust, Tar, Soil, 1736; cave sediment) on polymerase activity of 2D9 and ATaqG as determined by a PCR assay in the presence of decreasing amounts of inhibitor and plotted as the average relative resistance ( n = 3, standard error) (as in Figure 4 ) of 2D9 and ATaqG with respect to ATaqG as a reference (resistance = 1). ( b ) The effect of manual hot-start on resistance of 2D9 polymerase to inhibitors tar and soil at minimal limiting amounts of inhibitor. Hot-start increases inhibitor resistance by at least a factor of two.
    Amplitaq 360 Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    PerkinElmer amplitaq dna polymerase
    Comparison of the 16S-rRNA-nested-PCR assay with primer pairs ge3a-ge10 and ge9-ge2 with commercially available kits from three manufacturers: (A) PerkinElmer GeneAmp PCR reagent kit with <t>AmpliTaq</t> <t>DNA</t> polymerase; (B) Amersham Pharmacia Ready-To-Go PCR beads; (C) Qiagen Taq PCR Master Mix kit. Lanes 1 through 5 represent amplifications from a 10-fold dilution series ranging from 5 × 10 5 A. phagocytophilum -infected cells/ml to 50 infected cells/ml, and lane 6 contains DNA from uninfected human blood as a negative control. Phage ϕX174 DNA digested with Hae III was run in the lanes labeled M. The arrows indicate the position of the 546-bp amplified products.
    Amplitaq Dna Polymerase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 93/100, based on 3905 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Roche amplitaq dna polymerase
    Detection of the beta-lactamase gene in commercial Taq polymerase. Four dilutions of <t>Amplitaq</t> <t>DNA</t> polymerase were tested with primers for the beta-lactamase gene in the presence of 10 3 pUC19 plasmids (labeled 1000 pUC19 genomes) or H 2 O.
    Amplitaq Dna Polymerase, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 478 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Promega amplitaq dna polymerase
    Detection of the beta-lactamase gene in commercial Taq polymerase. Four dilutions of <t>Amplitaq</t> <t>DNA</t> polymerase were tested with primers for the beta-lactamase gene in the presence of 10 3 pUC19 plasmids (labeled 1000 pUC19 genomes) or H 2 O.
    Amplitaq Dna Polymerase, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher amplitaq 360 dna polymerase protocol
    Detection of the beta-lactamase gene in commercial Taq polymerase. Four dilutions of <t>Amplitaq</t> <t>DNA</t> polymerase were tested with primers for the beta-lactamase gene in the presence of 10 3 pUC19 plasmids (labeled 1000 pUC19 genomes) or H 2 O.
    Amplitaq 360 Dna Polymerase Protocol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Fisher Scientific amplitaq dna polymerase
    Detection of the beta-lactamase gene in commercial Taq polymerase. Four dilutions of <t>Amplitaq</t> <t>DNA</t> polymerase were tested with primers for the beta-lactamase gene in the presence of 10 3 pUC19 plasmids (labeled 1000 pUC19 genomes) or H 2 O.
    Amplitaq Dna Polymerase, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher amplitaq gold360
    Detection of the beta-lactamase gene in commercial Taq polymerase. Four dilutions of <t>Amplitaq</t> <t>DNA</t> polymerase were tested with primers for the beta-lactamase gene in the presence of 10 3 pUC19 plasmids (labeled 1000 pUC19 genomes) or H 2 O.
    Amplitaq Gold360, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    TaKaRa amplitaq dna polymerase
    Detection of the beta-lactamase gene in commercial Taq polymerase. Four dilutions of <t>Amplitaq</t> <t>DNA</t> polymerase were tested with primers for the beta-lactamase gene in the presence of 10 3 pUC19 plasmids (labeled 1000 pUC19 genomes) or H 2 O.
    Amplitaq Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher amplitaq dna polymerase ld
    Detection of the beta-lactamase gene in commercial Taq polymerase. Four dilutions of <t>Amplitaq</t> <t>DNA</t> polymerase were tested with primers for the beta-lactamase gene in the presence of 10 3 pUC19 plasmids (labeled 1000 pUC19 genomes) or H 2 O.
    Amplitaq Dna Polymerase Ld, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    PerkinElmer amplitaq thermostable dna polymerase
    Detection of the beta-lactamase gene in commercial Taq polymerase. Four dilutions of <t>Amplitaq</t> <t>DNA</t> polymerase were tested with primers for the beta-lactamase gene in the presence of 10 3 pUC19 plasmids (labeled 1000 pUC19 genomes) or H 2 O.
    Amplitaq Thermostable Dna Polymerase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 85/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    PerkinElmer amplitaq gold dna polymerase
    Amplification of eight copies of HPV-16 accomplished in the presence of TE buffer (lanes b, c, and d), 0.04% (final concentration [vol/vol]) NP-40 and 0.04% Tween (lanes e and f), 0.08% Tween 20 (lanes g and h), and 0.08% NP-40 (lanes i and j). The negative control is in lane a. Amplification was done with <t>AmpliTaq</t> Gold <t>DNA</t> polymerase in a model 9600 DNA thermal cycler. A 450-bp band is visible in positive reactions.
    Amplitaq Gold Dna Polymerase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 93/100, based on 1608 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Roche amplitaq gold dna polymerase
    Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); <t>Amplitaq</t> = AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); OneTaq = OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); HotStarTaq = HotStarTaq <t>DNA</t> Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.
    Amplitaq Gold Dna Polymerase, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 437 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Bio-Rad amplitaq dna polymerase
    Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); <t>Amplitaq</t> = AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); OneTaq = OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); HotStarTaq = HotStarTaq <t>DNA</t> Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.
    Amplitaq Dna Polymerase, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Confronting two-pair primers–polymerase chain reaction for NRF2 -617C/A and -653A/G was carried out at the indicated T a using 2.5U/sample of AmpliTaq Gold polymerase. N: Negative control indicates DNA template omission. MW: Molecular weights (100 bp up to 1000 bp).

    Journal: Future Science OA

    Article Title: Confronting two biomolecular techniques to detect NRF2 gene polymorphism biomarkers

    doi: 10.4155/fsoa-2018-0075

    Figure Lengend Snippet: Confronting two-pair primers–polymerase chain reaction for NRF2 -617C/A and -653A/G was carried out at the indicated T a using 2.5U/sample of AmpliTaq Gold polymerase. N: Negative control indicates DNA template omission. MW: Molecular weights (100 bp up to 1000 bp).

    Article Snippet: All PCRs were performed with 1 unit/sample of Kapa Hot Start Taq polymerase, Biosystems Cat. KK1508 (Sigma-Aldrich, MO, USA) except for the PCR shown in where 2.5 units/sample of AmpliTaq Gold polymerase, Applied Biosystems Cat. N8080161 (ThermoFisher Scientific, MA, USA) were used.

    Techniques: Polymerase Chain Reaction, Negative Control

    Inhibition and the effect of hot-start. ( a ) Inhibition profiles of 2D9 versus AmpliTaq Gold 360 (ATaqG) in PCR. The effect of various inhibitors (SP61: bone dust, Tar, Soil, 1736; cave sediment) on polymerase activity of 2D9 and ATaqG as determined by a PCR assay in the presence of decreasing amounts of inhibitor and plotted as the average relative resistance ( n = 3, standard error) (as in Figure 4 ) of 2D9 and ATaqG with respect to ATaqG as a reference (resistance = 1). ( b ) The effect of manual hot-start on resistance of 2D9 polymerase to inhibitors tar and soil at minimal limiting amounts of inhibitor. Hot-start increases inhibitor resistance by at least a factor of two.

    Journal: Nucleic Acids Research

    Article Title: Molecular breeding of polymerases for resistance to environmental inhibitors

    doi: 10.1093/nar/gkq1360

    Figure Lengend Snippet: Inhibition and the effect of hot-start. ( a ) Inhibition profiles of 2D9 versus AmpliTaq Gold 360 (ATaqG) in PCR. The effect of various inhibitors (SP61: bone dust, Tar, Soil, 1736; cave sediment) on polymerase activity of 2D9 and ATaqG as determined by a PCR assay in the presence of decreasing amounts of inhibitor and plotted as the average relative resistance ( n = 3, standard error) (as in Figure 4 ) of 2D9 and ATaqG with respect to ATaqG as a reference (resistance = 1). ( b ) The effect of manual hot-start on resistance of 2D9 polymerase to inhibitors tar and soil at minimal limiting amounts of inhibitor. Hot-start increases inhibitor resistance by at least a factor of two.

    Article Snippet: Inhibition profiles for AmpliTaq Gold 360 DNA polymerase (Applied Biosystems) were determined using an identical PCR set-up but using AmpliTaq Gold buffer and thermocycling conditions according to manufacturer’s recommendations.

    Techniques: Inhibition, Polymerase Chain Reaction, Activity Assay

    Comparison of the 16S-rRNA-nested-PCR assay with primer pairs ge3a-ge10 and ge9-ge2 with commercially available kits from three manufacturers: (A) PerkinElmer GeneAmp PCR reagent kit with AmpliTaq DNA polymerase; (B) Amersham Pharmacia Ready-To-Go PCR beads; (C) Qiagen Taq PCR Master Mix kit. Lanes 1 through 5 represent amplifications from a 10-fold dilution series ranging from 5 × 10 5 A. phagocytophilum -infected cells/ml to 50 infected cells/ml, and lane 6 contains DNA from uninfected human blood as a negative control. Phage ϕX174 DNA digested with Hae III was run in the lanes labeled M. The arrows indicate the position of the 546-bp amplified products.

    Journal: Journal of Clinical Microbiology

    Article Title: Comparison of PCR Assays for Detection of the Agent of Human Granulocytic Ehrlichiosis, Anaplasma phagocytophilum

    doi: 10.1128/JCM.41.2.717-722.2003

    Figure Lengend Snippet: Comparison of the 16S-rRNA-nested-PCR assay with primer pairs ge3a-ge10 and ge9-ge2 with commercially available kits from three manufacturers: (A) PerkinElmer GeneAmp PCR reagent kit with AmpliTaq DNA polymerase; (B) Amersham Pharmacia Ready-To-Go PCR beads; (C) Qiagen Taq PCR Master Mix kit. Lanes 1 through 5 represent amplifications from a 10-fold dilution series ranging from 5 × 10 5 A. phagocytophilum -infected cells/ml to 50 infected cells/ml, and lane 6 contains DNA from uninfected human blood as a negative control. Phage ϕX174 DNA digested with Hae III was run in the lanes labeled M. The arrows indicate the position of the 546-bp amplified products.

    Article Snippet: The initial limit of detection testing used reagents from the GeneAmp PCR kit with AmpliTaq DNA polymerase (PerkinElmer, Foster City, Calif.).

    Techniques: Nested PCR, Polymerase Chain Reaction, Infection, Negative Control, Labeling, Amplification

    Detection of the beta-lactamase gene in commercial Taq polymerase. Four dilutions of Amplitaq DNA polymerase were tested with primers for the beta-lactamase gene in the presence of 10 3 pUC19 plasmids (labeled 1000 pUC19 genomes) or H 2 O.

    Journal: PLoS ONE

    Article Title: Optimizing Taq Polymerase Concentration for Improved Signal-to-Noise in the Broad Range Detection of Low Abundance Bacteria

    doi: 10.1371/journal.pone.0007010

    Figure Lengend Snippet: Detection of the beta-lactamase gene in commercial Taq polymerase. Four dilutions of Amplitaq DNA polymerase were tested with primers for the beta-lactamase gene in the presence of 10 3 pUC19 plasmids (labeled 1000 pUC19 genomes) or H 2 O.

    Article Snippet: Two polymerases not designed specifically for qPCR (no anti-Taq antibody) were also tested: Amplitaq DNA polymerase (ABI, CA; Roche lot # C00622); Qiagen Taq DNA polymerase (Qiagen, CA; Cat # 201205, lot # 127132149.

    Techniques: Labeling

    Amplification of eight copies of HPV-16 accomplished in the presence of TE buffer (lanes b, c, and d), 0.04% (final concentration [vol/vol]) NP-40 and 0.04% Tween (lanes e and f), 0.08% Tween 20 (lanes g and h), and 0.08% NP-40 (lanes i and j). The negative control is in lane a. Amplification was done with AmpliTaq Gold DNA polymerase in a model 9600 DNA thermal cycler. A 450-bp band is visible in positive reactions.

    Journal: Journal of Clinical Microbiology

    Article Title: Effect of Nonionic Detergents on Amplification of Human Papillomavirus DNA with Consensus Primers MY09 and MY11

    doi:

    Figure Lengend Snippet: Amplification of eight copies of HPV-16 accomplished in the presence of TE buffer (lanes b, c, and d), 0.04% (final concentration [vol/vol]) NP-40 and 0.04% Tween (lanes e and f), 0.08% Tween 20 (lanes g and h), and 0.08% NP-40 (lanes i and j). The negative control is in lane a. Amplification was done with AmpliTaq Gold DNA polymerase in a model 9600 DNA thermal cycler. A 450-bp band is visible in positive reactions.

    Article Snippet: Ten-microliter volumes of the detergent preparations described below were added to 90-μl volumes of a PCR master mix containing the usual components ( , ) plus eight copies of HPV-16 (kindly provided by H. zur Hausen), 5 U of AmpliTaq Gold DNA polymerase (Perkin-Elmer, Montréal, Canada), and the L1 consensus HPV primers MY09 and MY11.

    Techniques: Amplification, Concentration Assay, Negative Control

    Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); Amplitaq = AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); OneTaq = OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); HotStarTaq = HotStarTaq DNA Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.

    Journal: Scientific Reports

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay

    doi: 10.1038/s41598-019-40035-5

    Figure Lengend Snippet: Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); Amplitaq = AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); OneTaq = OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); HotStarTaq = HotStarTaq DNA Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.

    Article Snippet: Optimization of the multiplex oligonucleotide ligation step In the very first paper dealing with MOL-PCR by Deshpande et al ., ligation and PCR were conducted in a single reaction using Ampligase (Epicentre, Wisconsin, USA) and Amplitaq Gold DNA polymerase (Roche, Switzerland).

    Techniques: Polymerase Chain Reaction, Hot Start PCR