amplicon dna Search Results


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  • 99
    Thermo Fisher amplicons
    Bovine IGF2 gene and transcript structure with primer locations for amplification of promoter specific transcripts and splice variants. The exon-intron structure of bovine insulin/insulin-like growth factor 2 ( INS/IGF2 , GenBank accession no. EU518675.1) with locations of five promoters (P0, P1, P2, P3 and P4) is shown at the top with promoters ( IGF2 -P0 –P4) and splice variant specific transcripts indicated below. Red and green boxes depict untranslated and protein coding exons, respectively. Forward (F) and reverse (R) primers are indicated with region spanned, including intron where applicable, symbolized by a black bar between primers above the transcript. According to the transcription initiation site of human IGF2 -P0 transcript, the putative orthologous bovine transcript is predicted to originate from a highly conserved region located upstream of the splice donor site of transcript P1 exon 2. We could specifically amplify bovine IGF2 -P0 using a strategically designed forward primer within this unique 5’-UTR sequence and the reverse primer located within exon 2. The two splice variants of P1 promoter transcripts include leading exon 1 which is alternatively spliced onto exons 2 and 3 ( IGF2 -P1e2) and exon 3 ( IGF2 -P1e3) plus the coding exons. In order to amplify the P1 promoter transcripts, two pairs of primers located within exon 2 (for IGF2 -P1e2) and exons 3 and 8 (for IGF2 -P1e3) were used. This approach was necessary because specific amplification of transcripts derived from P1 promoter failed due to lack of suitable PCR primer sequence in exon 1. Since IGF2 -transcript P1 exon 2 is part of the first exonic region of transcript IGF2 -P0, and exon 3 is present in both IGF2- P0 and IGF2- P1 transcripts, the IGF2 -P1e2 and -P1e3 <t>amplicons</t> could potentially derive from P0 and/or P1 promoters, depending on tissue and developmental stage. We quantified transcript abundances for two splice variants derived from IGF2- P2 promoter which comprise leading exon 4 ( IGF2 -P2e4) or leading exons 4 and 5 ( IGF2 -P2e5) as well as the protein coding exons. The forward primer for IGF2 -P2e4 was designed to span the junction of exons 4 and 8, and for IGF2 -P2e5 was in exon 5 with the reverse primer for both splice variants in exon 8. To amplify IGF2 -P3 and IGF2 -P4 transcripts, forward primers were designed within exons 6 and 7 with the reverse primer located within exon 8. All primers are detailed in S3 Table .
    Amplicons, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 51844 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 51844 article reviews
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    amplicons - by Bioz Stars, 2020-08
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    99
    Millipore dna amplicons
    Bovine IGF2 gene and transcript structure with primer locations for amplification of promoter specific transcripts and splice variants. The exon-intron structure of bovine insulin/insulin-like growth factor 2 ( INS/IGF2 , GenBank accession no. EU518675.1) with locations of five promoters (P0, P1, P2, P3 and P4) is shown at the top with promoters ( IGF2 -P0 –P4) and splice variant specific transcripts indicated below. Red and green boxes depict untranslated and protein coding exons, respectively. Forward (F) and reverse (R) primers are indicated with region spanned, including intron where applicable, symbolized by a black bar between primers above the transcript. According to the transcription initiation site of human IGF2 -P0 transcript, the putative orthologous bovine transcript is predicted to originate from a highly conserved region located upstream of the splice donor site of transcript P1 exon 2. We could specifically amplify bovine IGF2 -P0 using a strategically designed forward primer within this unique 5’-UTR sequence and the reverse primer located within exon 2. The two splice variants of P1 promoter transcripts include leading exon 1 which is alternatively spliced onto exons 2 and 3 ( IGF2 -P1e2) and exon 3 ( IGF2 -P1e3) plus the coding exons. In order to amplify the P1 promoter transcripts, two pairs of primers located within exon 2 (for IGF2 -P1e2) and exons 3 and 8 (for IGF2 -P1e3) were used. This approach was necessary because specific amplification of transcripts derived from P1 promoter failed due to lack of suitable PCR primer sequence in exon 1. Since IGF2 -transcript P1 exon 2 is part of the first exonic region of transcript IGF2 -P0, and exon 3 is present in both IGF2- P0 and IGF2- P1 transcripts, the IGF2 -P1e2 and -P1e3 <t>amplicons</t> could potentially derive from P0 and/or P1 promoters, depending on tissue and developmental stage. We quantified transcript abundances for two splice variants derived from IGF2- P2 promoter which comprise leading exon 4 ( IGF2 -P2e4) or leading exons 4 and 5 ( IGF2 -P2e5) as well as the protein coding exons. The forward primer for IGF2 -P2e4 was designed to span the junction of exons 4 and 8, and for IGF2 -P2e5 was in exon 5 with the reverse primer for both splice variants in exon 8. To amplify IGF2 -P3 and IGF2 -P4 transcripts, forward primers were designed within exons 6 and 7 with the reverse primer located within exon 8. All primers are detailed in S3 Table .
    Dna Amplicons, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore dna amplicon
    Bovine IGF2 gene and transcript structure with primer locations for amplification of promoter specific transcripts and splice variants. The exon-intron structure of bovine insulin/insulin-like growth factor 2 ( INS/IGF2 , GenBank accession no. EU518675.1) with locations of five promoters (P0, P1, P2, P3 and P4) is shown at the top with promoters ( IGF2 -P0 –P4) and splice variant specific transcripts indicated below. Red and green boxes depict untranslated and protein coding exons, respectively. Forward (F) and reverse (R) primers are indicated with region spanned, including intron where applicable, symbolized by a black bar between primers above the transcript. According to the transcription initiation site of human IGF2 -P0 transcript, the putative orthologous bovine transcript is predicted to originate from a highly conserved region located upstream of the splice donor site of transcript P1 exon 2. We could specifically amplify bovine IGF2 -P0 using a strategically designed forward primer within this unique 5’-UTR sequence and the reverse primer located within exon 2. The two splice variants of P1 promoter transcripts include leading exon 1 which is alternatively spliced onto exons 2 and 3 ( IGF2 -P1e2) and exon 3 ( IGF2 -P1e3) plus the coding exons. In order to amplify the P1 promoter transcripts, two pairs of primers located within exon 2 (for IGF2 -P1e2) and exons 3 and 8 (for IGF2 -P1e3) were used. This approach was necessary because specific amplification of transcripts derived from P1 promoter failed due to lack of suitable PCR primer sequence in exon 1. Since IGF2 -transcript P1 exon 2 is part of the first exonic region of transcript IGF2 -P0, and exon 3 is present in both IGF2- P0 and IGF2- P1 transcripts, the IGF2 -P1e2 and -P1e3 <t>amplicons</t> could potentially derive from P0 and/or P1 promoters, depending on tissue and developmental stage. We quantified transcript abundances for two splice variants derived from IGF2- P2 promoter which comprise leading exon 4 ( IGF2 -P2e4) or leading exons 4 and 5 ( IGF2 -P2e5) as well as the protein coding exons. The forward primer for IGF2 -P2e4 was designed to span the junction of exons 4 and 8, and for IGF2 -P2e5 was in exon 5 with the reverse primer for both splice variants in exon 8. To amplify IGF2 -P3 and IGF2 -P4 transcripts, forward primers were designed within exons 6 and 7 with the reverse primer located within exon 8. All primers are detailed in S3 Table .
    Dna Amplicon, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Genewiz amplicon dna
    Bovine IGF2 gene and transcript structure with primer locations for amplification of promoter specific transcripts and splice variants. The exon-intron structure of bovine insulin/insulin-like growth factor 2 ( INS/IGF2 , GenBank accession no. EU518675.1) with locations of five promoters (P0, P1, P2, P3 and P4) is shown at the top with promoters ( IGF2 -P0 –P4) and splice variant specific transcripts indicated below. Red and green boxes depict untranslated and protein coding exons, respectively. Forward (F) and reverse (R) primers are indicated with region spanned, including intron where applicable, symbolized by a black bar between primers above the transcript. According to the transcription initiation site of human IGF2 -P0 transcript, the putative orthologous bovine transcript is predicted to originate from a highly conserved region located upstream of the splice donor site of transcript P1 exon 2. We could specifically amplify bovine IGF2 -P0 using a strategically designed forward primer within this unique 5’-UTR sequence and the reverse primer located within exon 2. The two splice variants of P1 promoter transcripts include leading exon 1 which is alternatively spliced onto exons 2 and 3 ( IGF2 -P1e2) and exon 3 ( IGF2 -P1e3) plus the coding exons. In order to amplify the P1 promoter transcripts, two pairs of primers located within exon 2 (for IGF2 -P1e2) and exons 3 and 8 (for IGF2 -P1e3) were used. This approach was necessary because specific amplification of transcripts derived from P1 promoter failed due to lack of suitable PCR primer sequence in exon 1. Since IGF2 -transcript P1 exon 2 is part of the first exonic region of transcript IGF2 -P0, and exon 3 is present in both IGF2- P0 and IGF2- P1 transcripts, the IGF2 -P1e2 and -P1e3 <t>amplicons</t> could potentially derive from P0 and/or P1 promoters, depending on tissue and developmental stage. We quantified transcript abundances for two splice variants derived from IGF2- P2 promoter which comprise leading exon 4 ( IGF2 -P2e4) or leading exons 4 and 5 ( IGF2 -P2e5) as well as the protein coding exons. The forward primer for IGF2 -P2e4 was designed to span the junction of exons 4 and 8, and for IGF2 -P2e5 was in exon 5 with the reverse primer for both splice variants in exon 8. To amplify IGF2 -P3 and IGF2 -P4 transcripts, forward primers were designed within exons 6 and 7 with the reverse primer located within exon 8. All primers are detailed in S3 Table .
    Amplicon Dna, supplied by Genewiz, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Illumina Inc dna amplicons
    Bovine IGF2 gene and transcript structure with primer locations for amplification of promoter specific transcripts and splice variants. The exon-intron structure of bovine insulin/insulin-like growth factor 2 ( INS/IGF2 , GenBank accession no. EU518675.1) with locations of five promoters (P0, P1, P2, P3 and P4) is shown at the top with promoters ( IGF2 -P0 –P4) and splice variant specific transcripts indicated below. Red and green boxes depict untranslated and protein coding exons, respectively. Forward (F) and reverse (R) primers are indicated with region spanned, including intron where applicable, symbolized by a black bar between primers above the transcript. According to the transcription initiation site of human IGF2 -P0 transcript, the putative orthologous bovine transcript is predicted to originate from a highly conserved region located upstream of the splice donor site of transcript P1 exon 2. We could specifically amplify bovine IGF2 -P0 using a strategically designed forward primer within this unique 5’-UTR sequence and the reverse primer located within exon 2. The two splice variants of P1 promoter transcripts include leading exon 1 which is alternatively spliced onto exons 2 and 3 ( IGF2 -P1e2) and exon 3 ( IGF2 -P1e3) plus the coding exons. In order to amplify the P1 promoter transcripts, two pairs of primers located within exon 2 (for IGF2 -P1e2) and exons 3 and 8 (for IGF2 -P1e3) were used. This approach was necessary because specific amplification of transcripts derived from P1 promoter failed due to lack of suitable PCR primer sequence in exon 1. Since IGF2 -transcript P1 exon 2 is part of the first exonic region of transcript IGF2 -P0, and exon 3 is present in both IGF2- P0 and IGF2- P1 transcripts, the IGF2 -P1e2 and -P1e3 <t>amplicons</t> could potentially derive from P0 and/or P1 promoters, depending on tissue and developmental stage. We quantified transcript abundances for two splice variants derived from IGF2- P2 promoter which comprise leading exon 4 ( IGF2 -P2e4) or leading exons 4 and 5 ( IGF2 -P2e5) as well as the protein coding exons. The forward primer for IGF2 -P2e4 was designed to span the junction of exons 4 and 8, and for IGF2 -P2e5 was in exon 5 with the reverse primer for both splice variants in exon 8. To amplify IGF2 -P3 and IGF2 -P4 transcripts, forward primers were designed within exons 6 and 7 with the reverse primer located within exon 8. All primers are detailed in S3 Table .
    Dna Amplicons, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 227 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Roche amplicon dna
    Bovine IGF2 gene and transcript structure with primer locations for amplification of promoter specific transcripts and splice variants. The exon-intron structure of bovine insulin/insulin-like growth factor 2 ( INS/IGF2 , GenBank accession no. EU518675.1) with locations of five promoters (P0, P1, P2, P3 and P4) is shown at the top with promoters ( IGF2 -P0 –P4) and splice variant specific transcripts indicated below. Red and green boxes depict untranslated and protein coding exons, respectively. Forward (F) and reverse (R) primers are indicated with region spanned, including intron where applicable, symbolized by a black bar between primers above the transcript. According to the transcription initiation site of human IGF2 -P0 transcript, the putative orthologous bovine transcript is predicted to originate from a highly conserved region located upstream of the splice donor site of transcript P1 exon 2. We could specifically amplify bovine IGF2 -P0 using a strategically designed forward primer within this unique 5’-UTR sequence and the reverse primer located within exon 2. The two splice variants of P1 promoter transcripts include leading exon 1 which is alternatively spliced onto exons 2 and 3 ( IGF2 -P1e2) and exon 3 ( IGF2 -P1e3) plus the coding exons. In order to amplify the P1 promoter transcripts, two pairs of primers located within exon 2 (for IGF2 -P1e2) and exons 3 and 8 (for IGF2 -P1e3) were used. This approach was necessary because specific amplification of transcripts derived from P1 promoter failed due to lack of suitable PCR primer sequence in exon 1. Since IGF2 -transcript P1 exon 2 is part of the first exonic region of transcript IGF2 -P0, and exon 3 is present in both IGF2- P0 and IGF2- P1 transcripts, the IGF2 -P1e2 and -P1e3 <t>amplicons</t> could potentially derive from P0 and/or P1 promoters, depending on tissue and developmental stage. We quantified transcript abundances for two splice variants derived from IGF2- P2 promoter which comprise leading exon 4 ( IGF2 -P2e4) or leading exons 4 and 5 ( IGF2 -P2e5) as well as the protein coding exons. The forward primer for IGF2 -P2e4 was designed to span the junction of exons 4 and 8, and for IGF2 -P2e5 was in exon 5 with the reverse primer for both splice variants in exon 8. To amplify IGF2 -P3 and IGF2 -P4 transcripts, forward primers were designed within exons 6 and 7 with the reverse primer located within exon 8. All primers are detailed in S3 Table .
    Amplicon Dna, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Macrogen dna amplicons
    Bovine IGF2 gene and transcript structure with primer locations for amplification of promoter specific transcripts and splice variants. The exon-intron structure of bovine insulin/insulin-like growth factor 2 ( INS/IGF2 , GenBank accession no. EU518675.1) with locations of five promoters (P0, P1, P2, P3 and P4) is shown at the top with promoters ( IGF2 -P0 –P4) and splice variant specific transcripts indicated below. Red and green boxes depict untranslated and protein coding exons, respectively. Forward (F) and reverse (R) primers are indicated with region spanned, including intron where applicable, symbolized by a black bar between primers above the transcript. According to the transcription initiation site of human IGF2 -P0 transcript, the putative orthologous bovine transcript is predicted to originate from a highly conserved region located upstream of the splice donor site of transcript P1 exon 2. We could specifically amplify bovine IGF2 -P0 using a strategically designed forward primer within this unique 5’-UTR sequence and the reverse primer located within exon 2. The two splice variants of P1 promoter transcripts include leading exon 1 which is alternatively spliced onto exons 2 and 3 ( IGF2 -P1e2) and exon 3 ( IGF2 -P1e3) plus the coding exons. In order to amplify the P1 promoter transcripts, two pairs of primers located within exon 2 (for IGF2 -P1e2) and exons 3 and 8 (for IGF2 -P1e3) were used. This approach was necessary because specific amplification of transcripts derived from P1 promoter failed due to lack of suitable PCR primer sequence in exon 1. Since IGF2 -transcript P1 exon 2 is part of the first exonic region of transcript IGF2 -P0, and exon 3 is present in both IGF2- P0 and IGF2- P1 transcripts, the IGF2 -P1e2 and -P1e3 <t>amplicons</t> could potentially derive from P0 and/or P1 promoters, depending on tissue and developmental stage. We quantified transcript abundances for two splice variants derived from IGF2- P2 promoter which comprise leading exon 4 ( IGF2 -P2e4) or leading exons 4 and 5 ( IGF2 -P2e5) as well as the protein coding exons. The forward primer for IGF2 -P2e4 was designed to span the junction of exons 4 and 8, and for IGF2 -P2e5 was in exon 5 with the reverse primer for both splice variants in exon 8. To amplify IGF2 -P3 and IGF2 -P4 transcripts, forward primers were designed within exons 6 and 7 with the reverse primer located within exon 8. All primers are detailed in S3 Table .
    Dna Amplicons, supplied by Macrogen, used in various techniques. Bioz Stars score: 92/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    GATC Biotech dna amplicons
    Bovine IGF2 gene and transcript structure with primer locations for amplification of promoter specific transcripts and splice variants. The exon-intron structure of bovine insulin/insulin-like growth factor 2 ( INS/IGF2 , GenBank accession no. EU518675.1) with locations of five promoters (P0, P1, P2, P3 and P4) is shown at the top with promoters ( IGF2 -P0 –P4) and splice variant specific transcripts indicated below. Red and green boxes depict untranslated and protein coding exons, respectively. Forward (F) and reverse (R) primers are indicated with region spanned, including intron where applicable, symbolized by a black bar between primers above the transcript. According to the transcription initiation site of human IGF2 -P0 transcript, the putative orthologous bovine transcript is predicted to originate from a highly conserved region located upstream of the splice donor site of transcript P1 exon 2. We could specifically amplify bovine IGF2 -P0 using a strategically designed forward primer within this unique 5’-UTR sequence and the reverse primer located within exon 2. The two splice variants of P1 promoter transcripts include leading exon 1 which is alternatively spliced onto exons 2 and 3 ( IGF2 -P1e2) and exon 3 ( IGF2 -P1e3) plus the coding exons. In order to amplify the P1 promoter transcripts, two pairs of primers located within exon 2 (for IGF2 -P1e2) and exons 3 and 8 (for IGF2 -P1e3) were used. This approach was necessary because specific amplification of transcripts derived from P1 promoter failed due to lack of suitable PCR primer sequence in exon 1. Since IGF2 -transcript P1 exon 2 is part of the first exonic region of transcript IGF2 -P0, and exon 3 is present in both IGF2- P0 and IGF2- P1 transcripts, the IGF2 -P1e2 and -P1e3 <t>amplicons</t> could potentially derive from P0 and/or P1 promoters, depending on tissue and developmental stage. We quantified transcript abundances for two splice variants derived from IGF2- P2 promoter which comprise leading exon 4 ( IGF2 -P2e4) or leading exons 4 and 5 ( IGF2 -P2e5) as well as the protein coding exons. The forward primer for IGF2 -P2e4 was designed to span the junction of exons 4 and 8, and for IGF2 -P2e5 was in exon 5 with the reverse primer for both splice variants in exon 8. To amplify IGF2 -P3 and IGF2 -P4 transcripts, forward primers were designed within exons 6 and 7 with the reverse primer located within exon 8. All primers are detailed in S3 Table .
    Dna Amplicons, supplied by GATC Biotech, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher amplicon dna
    <t>DNA</t> <t>amplicon</t> purification gel Desired amplicons are indicated (above 100bp marker). Undesired adaptor-adaptor products are also present but at a reduced level compared to the DNA amplicons.
    Amplicon Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 576 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    LifeSequencing dna amplicon
    <t>DNA</t> <t>amplicon</t> purification gel Desired amplicons are indicated (above 100bp marker). Undesired adaptor-adaptor products are also present but at a reduced level compared to the DNA amplicons.
    Dna Amplicon, supplied by LifeSequencing, used in various techniques. Bioz Stars score: 89/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Pacific Biosciences dna amplicons
    <t>DNA</t> <t>amplicon</t> purification gel Desired amplicons are indicated (above 100bp marker). Undesired adaptor-adaptor products are also present but at a reduced level compared to the DNA amplicons.
    Dna Amplicons, supplied by Pacific Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Fuji Pharma dna amplicons
    Electrophoresis images of <t>DNA</t> extracted from the C. muytjensii cells with or without fixation followed by direct qPCR producing long DNA <t>amplicons.</t> M: 100 bp DNA ladder; B: fixation B (methanol/acetic acid = 3/1); S: no fixation (use of physiological saline); Target gene length: principally 2,451 bp of the 16 S to 23 S rRNA gene.
    Dna Amplicons, supplied by Fuji Pharma, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    SeqWright amplicon dna
    Electrophoresis images of <t>DNA</t> extracted from the C. muytjensii cells with or without fixation followed by direct qPCR producing long DNA <t>amplicons.</t> M: 100 bp DNA ladder; B: fixation B (methanol/acetic acid = 3/1); S: no fixation (use of physiological saline); Target gene length: principally 2,451 bp of the 16 S to 23 S rRNA gene.
    Amplicon Dna, supplied by SeqWright, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Illumina Inc 16s amplicon dna
    Electrophoresis images of <t>DNA</t> extracted from the C. muytjensii cells with or without fixation followed by direct qPCR producing long DNA <t>amplicons.</t> M: 100 bp DNA ladder; B: fixation B (methanol/acetic acid = 3/1); S: no fixation (use of physiological saline); Target gene length: principally 2,451 bp of the 16 S to 23 S rRNA gene.
    16s Amplicon Dna, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    GE Healthcare competitor dna amplicons
    Electrophoresis images of <t>DNA</t> extracted from the C. muytjensii cells with or without fixation followed by direct qPCR producing long DNA <t>amplicons.</t> M: 100 bp DNA ladder; B: fixation B (methanol/acetic acid = 3/1); S: no fixation (use of physiological saline); Target gene length: principally 2,451 bp of the 16 S to 23 S rRNA gene.
    Competitor Dna Amplicons, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Bio-Synthesis Inc synthetic dna sdna amplicon oligonucleotides
    Electrophoresis images of <t>DNA</t> extracted from the C. muytjensii cells with or without fixation followed by direct qPCR producing long DNA <t>amplicons.</t> M: 100 bp DNA ladder; B: fixation B (methanol/acetic acid = 3/1); S: no fixation (use of physiological saline); Target gene length: principally 2,451 bp of the 16 S to 23 S rRNA gene.
    Synthetic Dna Sdna Amplicon Oligonucleotides, supplied by Bio-Synthesis Inc, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher synthetic amplicon dna
    Electrophoresis images of <t>DNA</t> extracted from the C. muytjensii cells with or without fixation followed by direct qPCR producing long DNA <t>amplicons.</t> M: 100 bp DNA ladder; B: fixation B (methanol/acetic acid = 3/1); S: no fixation (use of physiological saline); Target gene length: principally 2,451 bp of the 16 S to 23 S rRNA gene.
    Synthetic Amplicon Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Novartis synthetic dna amplicons
    Electrophoresis images of <t>DNA</t> extracted from the C. muytjensii cells with or without fixation followed by direct qPCR producing long DNA <t>amplicons.</t> M: 100 bp DNA ladder; B: fixation B (methanol/acetic acid = 3/1); S: no fixation (use of physiological saline); Target gene length: principally 2,451 bp of the 16 S to 23 S rRNA gene.
    Synthetic Dna Amplicons, supplied by Novartis, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TIB MOLBIOL dna cloned amplicon
    Electrophoresis images of <t>DNA</t> extracted from the C. muytjensii cells with or without fixation followed by direct qPCR producing long DNA <t>amplicons.</t> M: 100 bp DNA ladder; B: fixation B (methanol/acetic acid = 3/1); S: no fixation (use of physiological saline); Target gene length: principally 2,451 bp of the 16 S to 23 S rRNA gene.
    Dna Cloned Amplicon, supplied by TIB MOLBIOL, used in various techniques. Bioz Stars score: 90/100, based on 132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna cloned amplicon/product/TIB MOLBIOL
    Average 90 stars, based on 132 article reviews
    Price from $9.99 to $1999.99
    dna cloned amplicon - by Bioz Stars, 2020-08
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    Bovine IGF2 gene and transcript structure with primer locations for amplification of promoter specific transcripts and splice variants. The exon-intron structure of bovine insulin/insulin-like growth factor 2 ( INS/IGF2 , GenBank accession no. EU518675.1) with locations of five promoters (P0, P1, P2, P3 and P4) is shown at the top with promoters ( IGF2 -P0 –P4) and splice variant specific transcripts indicated below. Red and green boxes depict untranslated and protein coding exons, respectively. Forward (F) and reverse (R) primers are indicated with region spanned, including intron where applicable, symbolized by a black bar between primers above the transcript. According to the transcription initiation site of human IGF2 -P0 transcript, the putative orthologous bovine transcript is predicted to originate from a highly conserved region located upstream of the splice donor site of transcript P1 exon 2. We could specifically amplify bovine IGF2 -P0 using a strategically designed forward primer within this unique 5’-UTR sequence and the reverse primer located within exon 2. The two splice variants of P1 promoter transcripts include leading exon 1 which is alternatively spliced onto exons 2 and 3 ( IGF2 -P1e2) and exon 3 ( IGF2 -P1e3) plus the coding exons. In order to amplify the P1 promoter transcripts, two pairs of primers located within exon 2 (for IGF2 -P1e2) and exons 3 and 8 (for IGF2 -P1e3) were used. This approach was necessary because specific amplification of transcripts derived from P1 promoter failed due to lack of suitable PCR primer sequence in exon 1. Since IGF2 -transcript P1 exon 2 is part of the first exonic region of transcript IGF2 -P0, and exon 3 is present in both IGF2- P0 and IGF2- P1 transcripts, the IGF2 -P1e2 and -P1e3 amplicons could potentially derive from P0 and/or P1 promoters, depending on tissue and developmental stage. We quantified transcript abundances for two splice variants derived from IGF2- P2 promoter which comprise leading exon 4 ( IGF2 -P2e4) or leading exons 4 and 5 ( IGF2 -P2e5) as well as the protein coding exons. The forward primer for IGF2 -P2e4 was designed to span the junction of exons 4 and 8, and for IGF2 -P2e5 was in exon 5 with the reverse primer for both splice variants in exon 8. To amplify IGF2 -P3 and IGF2 -P4 transcripts, forward primers were designed within exons 6 and 7 with the reverse primer located within exon 8. All primers are detailed in S3 Table .

    Journal: PLoS ONE

    Article Title: Atlas of tissue- and developmental stage specific gene expression for the bovine insulin-like growth factor (IGF) system

    doi: 10.1371/journal.pone.0200466

    Figure Lengend Snippet: Bovine IGF2 gene and transcript structure with primer locations for amplification of promoter specific transcripts and splice variants. The exon-intron structure of bovine insulin/insulin-like growth factor 2 ( INS/IGF2 , GenBank accession no. EU518675.1) with locations of five promoters (P0, P1, P2, P3 and P4) is shown at the top with promoters ( IGF2 -P0 –P4) and splice variant specific transcripts indicated below. Red and green boxes depict untranslated and protein coding exons, respectively. Forward (F) and reverse (R) primers are indicated with region spanned, including intron where applicable, symbolized by a black bar between primers above the transcript. According to the transcription initiation site of human IGF2 -P0 transcript, the putative orthologous bovine transcript is predicted to originate from a highly conserved region located upstream of the splice donor site of transcript P1 exon 2. We could specifically amplify bovine IGF2 -P0 using a strategically designed forward primer within this unique 5’-UTR sequence and the reverse primer located within exon 2. The two splice variants of P1 promoter transcripts include leading exon 1 which is alternatively spliced onto exons 2 and 3 ( IGF2 -P1e2) and exon 3 ( IGF2 -P1e3) plus the coding exons. In order to amplify the P1 promoter transcripts, two pairs of primers located within exon 2 (for IGF2 -P1e2) and exons 3 and 8 (for IGF2 -P1e3) were used. This approach was necessary because specific amplification of transcripts derived from P1 promoter failed due to lack of suitable PCR primer sequence in exon 1. Since IGF2 -transcript P1 exon 2 is part of the first exonic region of transcript IGF2 -P0, and exon 3 is present in both IGF2- P0 and IGF2- P1 transcripts, the IGF2 -P1e2 and -P1e3 amplicons could potentially derive from P0 and/or P1 promoters, depending on tissue and developmental stage. We quantified transcript abundances for two splice variants derived from IGF2- P2 promoter which comprise leading exon 4 ( IGF2 -P2e4) or leading exons 4 and 5 ( IGF2 -P2e5) as well as the protein coding exons. The forward primer for IGF2 -P2e4 was designed to span the junction of exons 4 and 8, and for IGF2 -P2e5 was in exon 5 with the reverse primer for both splice variants in exon 8. To amplify IGF2 -P3 and IGF2 -P4 transcripts, forward primers were designed within exons 6 and 7 with the reverse primer located within exon 8. All primers are detailed in S3 Table .

    Article Snippet: Target specificity and integrity was confirmed via sequencing of selected amplicons on a 3730xl DNA Analyzer (Applied Biosystems, Inc., Foster City, CA, USA), plots of the melting curve derived by Mastercycler® ep Realplex software (Eppendorf, Inc., Hamburg, Germany), and by electrophoresis of PCR products on a 2% agarose gel (Agarose low EEO, AppliChem GmbH, Darmstadt, Germany) and visual inspection under UV with Gel DocTM 1000 Single Wavelength Mini-Transilluminator, using Quantity One image analyzing software (Bio-Rad Laboratories, Inc., Hercules, CA, USA) after staining with GelRed™ Nucleic Acid Stain (Biotium, Inc., Hayward, CA, USA).

    Techniques: Amplification, Variant Assay, Sequencing, Derivative Assay, Polymerase Chain Reaction

    DNA amplicon purification gel Desired amplicons are indicated (above 100bp marker). Undesired adaptor-adaptor products are also present but at a reduced level compared to the DNA amplicons.

    Journal: Bio-protocol

    Article Title: Preparation of Multiplexed Small RNA Libraries From Plants

    doi:

    Figure Lengend Snippet: DNA amplicon purification gel Desired amplicons are indicated (above 100bp marker). Undesired adaptor-adaptor products are also present but at a reduced level compared to the DNA amplicons.

    Article Snippet: Fiber glass (Corning Incorporated, catalog number: 988-10144) Extra thick blot paper (Bio-Rad Laboratories, catalog number: 170-3969) Whatman DE81 ion exchange cellulose chromatography paper (Thermo Fisher Scientific, catalog number: 05-717-1A) GlycoBlue coprecipitant (Life Technologies, catalog number: AM9516) 100% ethanol 75% ethanol EB buffer (QIAGEN, catalog number: 19086) Qubit RNA HS Assay Kit for quantification of purified small RNAs (Life Technologies, catalog number: ) Qubit dsDNA HS Assay Kit for quantification of DNA amplicon (Life Technologies, catalog number: ) Low salt buffer (see Recipes) High salt buffer (see Recipes)

    Techniques: Amplification, Purification, Marker

    Electrophoresis images of DNA extracted from the C. muytjensii cells with or without fixation followed by direct qPCR producing long DNA amplicons. M: 100 bp DNA ladder; B: fixation B (methanol/acetic acid = 3/1); S: no fixation (use of physiological saline); Target gene length: principally 2,451 bp of the 16 S to 23 S rRNA gene.

    Journal: Scientific Reports

    Article Title: A novel mechanism for direct real-time polymerase chain reaction that does not require DNA isolation from prokaryotic cells

    doi: 10.1038/srep28000

    Figure Lengend Snippet: Electrophoresis images of DNA extracted from the C. muytjensii cells with or without fixation followed by direct qPCR producing long DNA amplicons. M: 100 bp DNA ladder; B: fixation B (methanol/acetic acid = 3/1); S: no fixation (use of physiological saline); Target gene length: principally 2,451 bp of the 16 S to 23 S rRNA gene.

    Article Snippet: The pellet was washed three times with 500 μl of saline to completely remove the long DNA amplicons potentially adsorbed onto the cell walls of C. muytjensii , and DNA purification was performed using the QuickGene SP kit DNA tissue to determine the presence of long DNA amplicons in the cells (Fuji Photo Film Co., Ltd., Tokyo, Japan).

    Techniques: Electrophoresis, Real-time Polymerase Chain Reaction