ampk inhibitor compound c Search Results


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  • 99
    Millipore ampk inhibitor compound c
    Ampk Inhibitor Compound C, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Tocris dorsomorphin
    Dorsomorphin, supplied by Tocris, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Selleck Chemicals ampk inhibitor compound c
    Ampk Inhibitor Compound C, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Stemgent dorsomorphin
    Differentiation of DE cells into pancreatic progenitor cells. (A) Quantitative PCR analysis of the expression of HNF6 and PDX1 at day 10. Expression levels were normalized to TBP expression. mRNA expression was relative to that in control cells at day 10. Control cells were treated with three factors for 4 days as shown in Fig. 4A , and then without factors for 6 days. Error bars indicate SD (n = 3). (B) Left: cells were treated with each factor for 6 days and then stained with an anti-PDX1 antibody at day 10. Right: percentage of PDX1 + cells among differentiated cells treated with each factor. Mean ± SD (n = 5–10). (C) Each cell line, H9, iPS (IMR90)-4 and iPS 253G1, was treated with five factors for 6 days as shown in Fig. 4A , and stained then with an anti-PDX1 antibody at day 10. Mean ± SD (n = 5–10). F10, 50 ng/ml FGF10; Nog, 50 ng/ml Noggin; Dor, 1 μM <t>dorsomorphin;</t> RA, 2 μM retinoic acid; FR, 3 μM FR180204. Scale bar, 100 μm.
    Dorsomorphin, supplied by Stemgent, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Differentiation of DE cells into pancreatic progenitor cells. (A) Quantitative PCR analysis of the expression of HNF6 and PDX1 at day 10. Expression levels were normalized to TBP expression. mRNA expression was relative to that in control cells at day 10. Control cells were treated with three factors for 4 days as shown in Fig. 4A , and then without factors for 6 days. Error bars indicate SD (n = 3). (B) Left: cells were treated with each factor for 6 days and then stained with an anti-PDX1 antibody at day 10. Right: percentage of PDX1 + cells among differentiated cells treated with each factor. Mean ± SD (n = 5–10). (C) Each cell line, H9, iPS (IMR90)-4 and iPS 253G1, was treated with five factors for 6 days as shown in Fig. 4A , and stained then with an anti-PDX1 antibody at day 10. Mean ± SD (n = 5–10). F10, 50 ng/ml FGF10; Nog, 50 ng/ml Noggin; Dor, 1 μM dorsomorphin; RA, 2 μM retinoic acid; FR, 3 μM FR180204. Scale bar, 100 μm.

    Journal: Scientific Reports

    Article Title: Endodermal differentiation of human pluripotent stem cells to insulin-producing cells in 3D culture

    doi: 10.1038/srep04488

    Figure Lengend Snippet: Differentiation of DE cells into pancreatic progenitor cells. (A) Quantitative PCR analysis of the expression of HNF6 and PDX1 at day 10. Expression levels were normalized to TBP expression. mRNA expression was relative to that in control cells at day 10. Control cells were treated with three factors for 4 days as shown in Fig. 4A , and then without factors for 6 days. Error bars indicate SD (n = 3). (B) Left: cells were treated with each factor for 6 days and then stained with an anti-PDX1 antibody at day 10. Right: percentage of PDX1 + cells among differentiated cells treated with each factor. Mean ± SD (n = 5–10). (C) Each cell line, H9, iPS (IMR90)-4 and iPS 253G1, was treated with five factors for 6 days as shown in Fig. 4A , and stained then with an anti-PDX1 antibody at day 10. Mean ± SD (n = 5–10). F10, 50 ng/ml FGF10; Nog, 50 ng/ml Noggin; Dor, 1 μM dorsomorphin; RA, 2 μM retinoic acid; FR, 3 μM FR180204. Scale bar, 100 μm.

    Article Snippet: Subsequently, the cells were washed with PBS(+),the supplements were changed to 50 ng/ml FGF10 (R & D systems), 50 ng/ml Noggin (R & D systems), 1 μM dorsomorphin (Stemgent), 2 μM retinoic acid (Sigma) and 3 μM FR1890204 (Calbiochem), and the cells were cultured for 3 days.

    Techniques: Real-time Polymerase Chain Reaction, Expressing, Staining