amiloride Millipore Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore amiloride
    Data are mean ± SE. IMP preference ratio for T1R1 WT (closed bars) and T1R1 KO (open bars) mice in three 48 h 2-bottle preference test <t>(IMP+amiloride</t> vs amiloride) as a function of IMP concentration. The sample size of each genotype is indicated
    Amiloride, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1860 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amiloride/product/Millipore
    Average 99 stars, based on 1860 article reviews
    Price from $9.99 to $1999.99
    amiloride - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Millipore glp 1
    Induction of glucose-dependent insulin secretion by (xGLP-E4). (A) INS-1 cells and (B) β-TC-6 cells were cultured in 24-well plates until 90% confluence. Cells were washed and incubated in Krebs-Ringer Bicarbonate (KRB) buffer and treated with glucose for 2 hours at 37℃. Cells were incubated with 10 nM glucagon-like peptide-1 <t>(GLP-1)</t> analog in KRB buffer containing low and high glucose for another 2 hours, and insulin secretion into the buffer was then determined. CTL, control; GLP-1, glucagon-like peptide-1; Exe-4, exendin-4; xGLP, Xenopus GLP-1.
    Glp 1, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1479 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glp 1/product/Millipore
    Average 99 stars, based on 1479 article reviews
    Price from $9.99 to $1999.99
    glp 1 - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Millipore n 3 dimethylaminopropyl n
    Induction of glucose-dependent insulin secretion by (xGLP-E4). (A) INS-1 cells and (B) β-TC-6 cells were cultured in 24-well plates until 90% confluence. Cells were washed and incubated in Krebs-Ringer Bicarbonate (KRB) buffer and treated with glucose for 2 hours at 37℃. Cells were incubated with 10 nM glucagon-like peptide-1 <t>(GLP-1)</t> analog in KRB buffer containing low and high glucose for another 2 hours, and insulin secretion into the buffer was then determined. CTL, control; GLP-1, glucagon-like peptide-1; Exe-4, exendin-4; xGLP, Xenopus GLP-1.
    N 3 Dimethylaminopropyl N, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 418 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n 3 dimethylaminopropyl n/product/Millipore
    Average 99 stars, based on 418 article reviews
    Price from $9.99 to $1999.99
    n 3 dimethylaminopropyl n - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    95
    Millipore rink amide 4 methylbenzhydrylamine resin
    Induction of glucose-dependent insulin secretion by (xGLP-E4). (A) INS-1 cells and (B) β-TC-6 cells were cultured in 24-well plates until 90% confluence. Cells were washed and incubated in Krebs-Ringer Bicarbonate (KRB) buffer and treated with glucose for 2 hours at 37℃. Cells were incubated with 10 nM glucagon-like peptide-1 <t>(GLP-1)</t> analog in KRB buffer containing low and high glucose for another 2 hours, and insulin secretion into the buffer was then determined. CTL, control; GLP-1, glucagon-like peptide-1; Exe-4, exendin-4; xGLP, Xenopus GLP-1.
    Rink Amide 4 Methylbenzhydrylamine Resin, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rink amide 4 methylbenzhydrylamine resin/product/Millipore
    Average 95 stars, based on 67 article reviews
    Price from $9.99 to $1999.99
    rink amide 4 methylbenzhydrylamine resin - by Bioz Stars, 2020-09
    95/100 stars
      Buy from Supplier

    99
    Millipore rink amide resin
    Induction of glucose-dependent insulin secretion by (xGLP-E4). (A) INS-1 cells and (B) β-TC-6 cells were cultured in 24-well plates until 90% confluence. Cells were washed and incubated in Krebs-Ringer Bicarbonate (KRB) buffer and treated with glucose for 2 hours at 37℃. Cells were incubated with 10 nM glucagon-like peptide-1 <t>(GLP-1)</t> analog in KRB buffer containing low and high glucose for another 2 hours, and insulin secretion into the buffer was then determined. CTL, control; GLP-1, glucagon-like peptide-1; Exe-4, exendin-4; xGLP, Xenopus GLP-1.
    Rink Amide Resin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 322 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rink amide resin/product/Millipore
    Average 99 stars, based on 322 article reviews
    Price from $9.99 to $1999.99
    rink amide resin - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Millipore forskolin
    Dexamethasone reduces Cftr channel activity in FDLE cells. Graphs represent the mean + SEM of I SC in response to 100 nM dexamethasone for 24, 48 and 72 h measured in Ussing chambers. A: <t>Forskolin-induced</t> I SC (n = 25–56, *** p
    Forskolin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 12279 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/forskolin/product/Millipore
    Average 99 stars, based on 12279 article reviews
    Price from $9.99 to $1999.99
    forskolin - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Millipore sunitinib malate
    Effects of STAT3 inhibitor on sorafenib- and <t>sunitinib-induced</t> cell growth inhibition. After pretreatment with Stattic (STAT3 inhibitor, 10 µM) or DMSO (solvent) for 20 min, HaCaT, Caki-1, and HepG2 cells were incubated in a medium that included sorafenib or sunitinib at the indicated concentrations for 48 h. Cell viability was determined by WST-8 colorimetric assay. *p
    Sunitinib Malate, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sunitinib malate/product/Millipore
    Average 99 stars, based on 42 article reviews
    Price from $9.99 to $1999.99
    sunitinib malate - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Millipore cytochalasin d
    Differential endocytosis of primary and secondary CPP in human monocyte-derived macrophages and aortic endothelial cells. Human monocyte-derived macrophages (hMDM) or human aortic endothelial cells (haEC) were treated with Alexa488-labeled primary or secondary CPP, both 100 μg/mL calcium content, synthesized from FCS/DMEM supplemented with phosphate (final conc. 3.5 mM). Monomeric labeled-fetuin-A was included as a comparator (1 mg/ml). Cell-associated fluorescence was measured by flow cytometry in fixed cells at the stated timepoints.  (A)  Endocyosis observed in hMDM and  (B)  haEC, respectively.  (C,D)  hMDM were pre-treated for 30–60 min with one of several inhibitors (vs. vehicle) or receptor blocking antibodies (vs. isotype control).  (C)  Pre-treatment with cytochalasin  (D) , an inhibitor of actin polymerization, markedly attenuated uptake of both primary and secondary CPP and (
    Cytochalasin D, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 9195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cytochalasin d/product/Millipore
    Average 99 stars, based on 9195 article reviews
    Price from $9.99 to $1999.99
    cytochalasin d - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    97
    Millipore fmoc protected amino acids
    Differential endocytosis of primary and secondary CPP in human monocyte-derived macrophages and aortic endothelial cells. Human monocyte-derived macrophages (hMDM) or human aortic endothelial cells (haEC) were treated with Alexa488-labeled primary or secondary CPP, both 100 μg/mL calcium content, synthesized from FCS/DMEM supplemented with phosphate (final conc. 3.5 mM). Monomeric labeled-fetuin-A was included as a comparator (1 mg/ml). Cell-associated fluorescence was measured by flow cytometry in fixed cells at the stated timepoints.  (A)  Endocyosis observed in hMDM and  (B)  haEC, respectively.  (C,D)  hMDM were pre-treated for 30–60 min with one of several inhibitors (vs. vehicle) or receptor blocking antibodies (vs. isotype control).  (C)  Pre-treatment with cytochalasin  (D) , an inhibitor of actin polymerization, markedly attenuated uptake of both primary and secondary CPP and (
    Fmoc Protected Amino Acids, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 475 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fmoc protected amino acids/product/Millipore
    Average 97 stars, based on 475 article reviews
    Price from $9.99 to $1999.99
    fmoc protected amino acids - by Bioz Stars, 2020-09
    97/100 stars
      Buy from Supplier

    99
    Millipore eipa
    Benzimidazolones enhance benzamil-sensitive I SC . Effects of <t>DC-EBIO</t> (0.3 mM) on benzamil- and <t>EIPA-sensitive</t> I SC of rat FDLE cell monolayers. The figure shows the current reduction caused by 10 μM benzamil and 100 μM EIPA. Mean treatment-associated changes in current ± SEM of 12 (control/benzamil), 9 (DC-EBIO/benzamil), 11 (control/EIPA) and 14 (DC-EBIO/EIPA) monolayers (* P
    Eipa, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 410 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eipa/product/Millipore
    Average 99 stars, based on 410 article reviews
    Price from $9.99 to $1999.99
    eipa - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Millipore chlorpromazine
    Benzimidazolones enhance benzamil-sensitive I SC . Effects of <t>DC-EBIO</t> (0.3 mM) on benzamil- and <t>EIPA-sensitive</t> I SC of rat FDLE cell monolayers. The figure shows the current reduction caused by 10 μM benzamil and 100 μM EIPA. Mean treatment-associated changes in current ± SEM of 12 (control/benzamil), 9 (DC-EBIO/benzamil), 11 (control/EIPA) and 14 (DC-EBIO/EIPA) monolayers (* P
    Chlorpromazine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 800 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chlorpromazine/product/Millipore
    Average 99 stars, based on 800 article reviews
    Price from $9.99 to $1999.99
    chlorpromazine - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Millipore anti sma
    Fig. 3. Activation of mesangial cell ERK, JNK/SAPK and <t>p38</t> MAPK pathways by ET-1. ( A ) Cells were treated with the indicated agonists for the indicated times. Crude extracts were prepared and subjected to SDS–PAGE and immunoblotting with phospho-MAPK-specific antibodies (top three panels) or total MAPK blots (bottom three panels). ( B ) The same as (A), except that cells were treated for longer time periods. ( C ) Activation of MKKs by ET-1. Cells were treated with the indicated stimuli as in (A). Extracts were prepared and subjected to SDS–PAGE and immunoblotting with the indicated phospho-MKK-specific antibodies (top three panels) or, as a loading control, with an antibody to smooth muscle actin <t>(SMA,</t> bottom panel). ( D ) The same as (C), except that blots were probed with anti-phospho-MEK only. ( E ) Activation of CREB by ET-1. Mesangial cells were treated with the indicated agonists for the indicated times, and crude extracts were subjected to SDS–PAGE and immunoblotting with phospho-CREB/ATF-1-specific antibody.
    Anti Sma, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 391 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti sma/product/Millipore
    Average 99 stars, based on 391 article reviews
    Price from $9.99 to $1999.99
    anti sma - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    96
    Millipore 1 1 carbonyldiimidazole cdi
    Schematic diagram of ( A ) cRGD-conjugated PAMAMG2-g-cyclodextrin (CDG2-cRGD) and ( B ) self-assembly of pDNA/CDG2-cRGD polyplex. Abbreviations: CD, cyclodextrin; CDG2, PAMAMG2-g-cyclodextrin; <t>CDI,</t> <t>1,1′-carbonyldiimidazole;</t> cRGD, cyclic arginylglycylaspartic acid peptide; DMSO, dimethyl sulfoxide; PAMAMG2, Generation 2 polyamidoamine; PBS, phosphate-buffered saline; pDNA, plasmid DNA; pVEGF, plasmid vector with the VEGF gene; SPDP, 3-(2-pyridyldithio)propionic acid N -hydroxysuccinimide ester; VEGF, vascular endothelial growth factor.
    1 1 Carbonyldiimidazole Cdi, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1 1 carbonyldiimidazole cdi/product/Millipore
    Average 96 stars, based on 183 article reviews
    Price from $9.99 to $1999.99
    1 1 carbonyldiimidazole cdi - by Bioz Stars, 2020-09
    96/100 stars
      Buy from Supplier

    99
    Millipore peptide synthesis
    Schematic diagram of ( A ) cRGD-conjugated PAMAMG2-g-cyclodextrin (CDG2-cRGD) and ( B ) self-assembly of pDNA/CDG2-cRGD polyplex. Abbreviations: CD, cyclodextrin; CDG2, PAMAMG2-g-cyclodextrin; <t>CDI,</t> <t>1,1′-carbonyldiimidazole;</t> cRGD, cyclic arginylglycylaspartic acid peptide; DMSO, dimethyl sulfoxide; PAMAMG2, Generation 2 polyamidoamine; PBS, phosphate-buffered saline; pDNA, plasmid DNA; pVEGF, plasmid vector with the VEGF gene; SPDP, 3-(2-pyridyldithio)propionic acid N -hydroxysuccinimide ester; VEGF, vascular endothelial growth factor.
    Peptide Synthesis, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 562 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peptide synthesis/product/Millipore
    Average 99 stars, based on 562 article reviews
    Price from $9.99 to $1999.99
    peptide synthesis - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    96
    Millipore n tris
    Schematic diagram of ( A ) cRGD-conjugated PAMAMG2-g-cyclodextrin (CDG2-cRGD) and ( B ) self-assembly of pDNA/CDG2-cRGD polyplex. Abbreviations: CD, cyclodextrin; CDG2, PAMAMG2-g-cyclodextrin; <t>CDI,</t> <t>1,1′-carbonyldiimidazole;</t> cRGD, cyclic arginylglycylaspartic acid peptide; DMSO, dimethyl sulfoxide; PAMAMG2, Generation 2 polyamidoamine; PBS, phosphate-buffered saline; pDNA, plasmid DNA; pVEGF, plasmid vector with the VEGF gene; SPDP, 3-(2-pyridyldithio)propionic acid N -hydroxysuccinimide ester; VEGF, vascular endothelial growth factor.
    N Tris, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 194 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n tris/product/Millipore
    Average 96 stars, based on 194 article reviews
    Price from $9.99 to $1999.99
    n tris - by Bioz Stars, 2020-09
    96/100 stars
      Buy from Supplier

    99
    Millipore propylene carbonate
    Schematic diagram of ( A ) cRGD-conjugated PAMAMG2-g-cyclodextrin (CDG2-cRGD) and ( B ) self-assembly of pDNA/CDG2-cRGD polyplex. Abbreviations: CD, cyclodextrin; CDG2, PAMAMG2-g-cyclodextrin; <t>CDI,</t> <t>1,1′-carbonyldiimidazole;</t> cRGD, cyclic arginylglycylaspartic acid peptide; DMSO, dimethyl sulfoxide; PAMAMG2, Generation 2 polyamidoamine; PBS, phosphate-buffered saline; pDNA, plasmid DNA; pVEGF, plasmid vector with the VEGF gene; SPDP, 3-(2-pyridyldithio)propionic acid N -hydroxysuccinimide ester; VEGF, vascular endothelial growth factor.
    Propylene Carbonate, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/propylene carbonate/product/Millipore
    Average 99 stars, based on 98 article reviews
    Price from $9.99 to $1999.99
    propylene carbonate - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Millipore wortmannin
    Insulin induces phosphorylation of rNCC at threonine 58 (A and B) Representative Western blot assays performed using anti Flag monoclonal antibody, the pNCC-T58 phosphoantibody, and β-actin antibody, as stated. Total proteins were extracted from Xenopus laevis oocytes injected with water or rNCC cRNA and exposed to insulin for 0, 5, 15 and 30 minutes (A), or to 15 minutes in the absence or presence of <t>wortmannin</t> (B) (C) A representative Western blot analysis of protein extracts from ex-vivo perfused rat-kidneys. The kidneys were perfused with isotonic control solution or with isotonic solution containing 0.250 U/mL of insulin. (D) Densitometry analysis of phosphor-NCC over total rNCC from three different Western blot analyses compiling a total of 10 controls and 16 insulin perfused kidneys. (*p
    Wortmannin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 9936 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wortmannin/product/Millipore
    Average 99 stars, based on 9936 article reviews
    Price from $9.99 to $1999.99
    wortmannin - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Millipore amiloride hydrochloride hydrate
    AP301 activation of ENaC requires pore-forming α - or δ -subunit coexpression. (A) <t>Amiloride</t> (10 µ M)-sensitive current in different subunits and subunit combinations expressed in HEK-293 cells. Cells were patched in the whole-cell
    Amiloride Hydrochloride Hydrate, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amiloride hydrochloride hydrate/product/Millipore
    Average 99 stars, based on 121 article reviews
    Price from $9.99 to $1999.99
    amiloride hydrochloride hydrate - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Millipore cathepsin l
    Molecular docking of analogues 1 and 32 within the active site of cathepsin L. (A) Analogue 1 and (B) analogue 32 are shown in ball and stick mode (C, gray; H, white; O, red; N, blue; S, yellow; F, light blue; Br, brown). <t>Cathepsin</t> L is shown in ribbon
    Cathepsin L, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 260 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cathepsin l/product/Millipore
    Average 99 stars, based on 260 article reviews
    Price from $9.99 to $1999.99
    cathepsin l - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Millipore bumetanide
    Summary of the effects of R-009 on Calu-3WT and Calu-3KD epithelia and illustrated in . The filled columns show SCC inhibition caused by <t>bumetanide</t> (100 μ M added basolaterally) and acetazolamide (100 μ M added both
    Bumetanide, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 681 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bumetanide/product/Millipore
    Average 99 stars, based on 681 article reviews
    Price from $9.99 to $1999.99
    bumetanide - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    95
    Millipore scriptaid
    HDACis present cytotoxicity effect on canine B-cell lymphoma CLBL-1 cells (6 × 10 4 ) were subjected to the indicated concentrations of HDACis - CI-994, panobinostat, SBHA, SAHA, <t>scriptaid,</t> trichostatin A and tubacin ( A – G ). After 24 h treatment, cell viability and proliferation were evaluated with WST-1 reagent. Two replicate wells were used to determinate each data point and three independent experiments were carried out in different days. Best-fit IC50 values of each HDACis were calculated using the log (inhibitor) vs response (variable slope) function ( H ).
    Scriptaid, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 137 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/scriptaid/product/Millipore
    Average 95 stars, based on 137 article reviews
    Price from $9.99 to $1999.99
    scriptaid - by Bioz Stars, 2020-09
    95/100 stars
      Buy from Supplier

    Image Search Results


    Data are mean ± SE. IMP preference ratio for T1R1 WT (closed bars) and T1R1 KO (open bars) mice in three 48 h 2-bottle preference test (IMP+amiloride vs amiloride) as a function of IMP concentration. The sample size of each genotype is indicated

    Journal: The Journal of Neuroscience

    Article Title: The Importance of the Presence of a 5′-Ribonucleotide and the Contribution of the T1R1 + T1R3 Heterodimer and an Additional Low-Affinity Receptor in the Taste Detection of l-Glutamate as Assessed Psychophysically

    doi: 10.1523/JNEUROSCI.0417-14.2014

    Figure Lengend Snippet: Data are mean ± SE. IMP preference ratio for T1R1 WT (closed bars) and T1R1 KO (open bars) mice in three 48 h 2-bottle preference test (IMP+amiloride vs amiloride) as a function of IMP concentration. The sample size of each genotype is indicated

    Article Snippet: The chemicals for discrimination training and detection testing were presented as follows: sodium chloride (NaCl; Fisher Scientific), l -glutamic acid monosodium salt hydrate (MSG; Sigma), MSG mixed with 100 μ m amiloride hydrochloride (Sigma), MSG mixed with 100 μ m amiloride and 2.5 m m inosine 5′-monophosphate disodium salt (IMP; Sigma), and 2.5 m m IMP mixed with 100 μ m amiloride.

    Techniques: Mouse Assay, Concentration Assay

    Data are mean ± SE of the overall percent correct scores for T1R1 (top left), T1R2 (top right), T1R3 (bottom left), and T1R2 + T1R3 (bottom right) groups on the 2 test days of a 2.5 m m IMP + 100 μ m amiloride versus 100 μ m amiloride

    Journal: The Journal of Neuroscience

    Article Title: The Importance of the Presence of a 5′-Ribonucleotide and the Contribution of the T1R1 + T1R3 Heterodimer and an Additional Low-Affinity Receptor in the Taste Detection of l-Glutamate as Assessed Psychophysically

    doi: 10.1523/JNEUROSCI.0417-14.2014

    Figure Lengend Snippet: Data are mean ± SE of the overall percent correct scores for T1R1 (top left), T1R2 (top right), T1R3 (bottom left), and T1R2 + T1R3 (bottom right) groups on the 2 test days of a 2.5 m m IMP + 100 μ m amiloride versus 100 μ m amiloride

    Article Snippet: The chemicals for discrimination training and detection testing were presented as follows: sodium chloride (NaCl; Fisher Scientific), l -glutamic acid monosodium salt hydrate (MSG; Sigma), MSG mixed with 100 μ m amiloride hydrochloride (Sigma), MSG mixed with 100 μ m amiloride and 2.5 m m inosine 5′-monophosphate disodium salt (IMP; Sigma), and 2.5 m m IMP mixed with 100 μ m amiloride.

    Techniques:

    Data are mean ± SE of the overall percent correct scores for T1R1 (top left), T1R2 (top right), T1R3 (bottom left), and T1R2 + T1R3 (bottom right) groups as a function of MSG concentration prepared with 100 μ m amiloride and 2.5 m m IMP.

    Journal: The Journal of Neuroscience

    Article Title: The Importance of the Presence of a 5′-Ribonucleotide and the Contribution of the T1R1 + T1R3 Heterodimer and an Additional Low-Affinity Receptor in the Taste Detection of l-Glutamate as Assessed Psychophysically

    doi: 10.1523/JNEUROSCI.0417-14.2014

    Figure Lengend Snippet: Data are mean ± SE of the overall percent correct scores for T1R1 (top left), T1R2 (top right), T1R3 (bottom left), and T1R2 + T1R3 (bottom right) groups as a function of MSG concentration prepared with 100 μ m amiloride and 2.5 m m IMP.

    Article Snippet: The chemicals for discrimination training and detection testing were presented as follows: sodium chloride (NaCl; Fisher Scientific), l -glutamic acid monosodium salt hydrate (MSG; Sigma), MSG mixed with 100 μ m amiloride hydrochloride (Sigma), MSG mixed with 100 μ m amiloride and 2.5 m m inosine 5′-monophosphate disodium salt (IMP; Sigma), and 2.5 m m IMP mixed with 100 μ m amiloride.

    Techniques: Concentration Assay

    Data are mean ± SE of the overall percent correct scores across training days to 0.6 m MSG mixed with 100 μ m amiloride for T1R1 (top), T1R2 (middle), and T1R3 (bottom) groups. Chance performance is 50%. Breaks in curves represent a return

    Journal: The Journal of Neuroscience

    Article Title: The Importance of the Presence of a 5′-Ribonucleotide and the Contribution of the T1R1 + T1R3 Heterodimer and an Additional Low-Affinity Receptor in the Taste Detection of l-Glutamate as Assessed Psychophysically

    doi: 10.1523/JNEUROSCI.0417-14.2014

    Figure Lengend Snippet: Data are mean ± SE of the overall percent correct scores across training days to 0.6 m MSG mixed with 100 μ m amiloride for T1R1 (top), T1R2 (middle), and T1R3 (bottom) groups. Chance performance is 50%. Breaks in curves represent a return

    Article Snippet: The chemicals for discrimination training and detection testing were presented as follows: sodium chloride (NaCl; Fisher Scientific), l -glutamic acid monosodium salt hydrate (MSG; Sigma), MSG mixed with 100 μ m amiloride hydrochloride (Sigma), MSG mixed with 100 μ m amiloride and 2.5 m m inosine 5′-monophosphate disodium salt (IMP; Sigma), and 2.5 m m IMP mixed with 100 μ m amiloride.

    Techniques:

    Induction of glucose-dependent insulin secretion by (xGLP-E4). (A) INS-1 cells and (B) β-TC-6 cells were cultured in 24-well plates until 90% confluence. Cells were washed and incubated in Krebs-Ringer Bicarbonate (KRB) buffer and treated with glucose for 2 hours at 37℃. Cells were incubated with 10 nM glucagon-like peptide-1 (GLP-1) analog in KRB buffer containing low and high glucose for another 2 hours, and insulin secretion into the buffer was then determined. CTL, control; GLP-1, glucagon-like peptide-1; Exe-4, exendin-4; xGLP, Xenopus GLP-1.

    Journal: Endocrinology and Metabolism

    Article Title: A Novel Long-Acting Glucagon-Like Peptide-1 Agonist with Improved Efficacy in Insulin Secretion and β-Cell Growth

    doi: 10.3803/EnM.2014.29.3.320

    Figure Lengend Snippet: Induction of glucose-dependent insulin secretion by (xGLP-E4). (A) INS-1 cells and (B) β-TC-6 cells were cultured in 24-well plates until 90% confluence. Cells were washed and incubated in Krebs-Ringer Bicarbonate (KRB) buffer and treated with glucose for 2 hours at 37℃. Cells were incubated with 10 nM glucagon-like peptide-1 (GLP-1) analog in KRB buffer containing low and high glucose for another 2 hours, and insulin secretion into the buffer was then determined. CTL, control; GLP-1, glucagon-like peptide-1; Exe-4, exendin-4; xGLP, Xenopus GLP-1.

    Article Snippet: Stability of GLP-1 analogs To evaluate the stability of GLP-1 and its analogs against DPP-IV activity, peptides were incubated at an initial concentration of 100 nM in DMEM containing 10% FBS or in 100% FBS at 37℃ for 0, 12, 24, 48, 72, and 96 hours in the presence or absence of 0.2 mM DPP-IV inhibitor diprotin A (Sigma-Aldrich, St Louis, MO, USA).

    Techniques: Cell Culture, Incubation, CTL Assay

    Stability of glucagon-like peptide-1 (GLP-1) analogs. The stability of GLP-1 and GLP-1 analogs against dipeptidyl peptidase-IV (DPP-IV) activity was evaluated by incubating the individual peptides in medium containing 10% fetal bovine serum (FBS) (A) or in 100% FBS (B). Peptides were incubated at an initial concentration of 100 nM in Dulbecco's Modified Eagle's medium containing 10% FBS or 100% FBS at 37℃ in the presence or absence of 0.2 mM of the peptidase inhibitor diprotin A. Peptide activity was then assessed by measuring luciferase activity in cells expressing GLP1R and CRE-luc. Results are presented as mean±standard error of the mean of at least three independent experiments.

    Journal: Endocrinology and Metabolism

    Article Title: A Novel Long-Acting Glucagon-Like Peptide-1 Agonist with Improved Efficacy in Insulin Secretion and β-Cell Growth

    doi: 10.3803/EnM.2014.29.3.320

    Figure Lengend Snippet: Stability of glucagon-like peptide-1 (GLP-1) analogs. The stability of GLP-1 and GLP-1 analogs against dipeptidyl peptidase-IV (DPP-IV) activity was evaluated by incubating the individual peptides in medium containing 10% fetal bovine serum (FBS) (A) or in 100% FBS (B). Peptides were incubated at an initial concentration of 100 nM in Dulbecco's Modified Eagle's medium containing 10% FBS or 100% FBS at 37℃ in the presence or absence of 0.2 mM of the peptidase inhibitor diprotin A. Peptide activity was then assessed by measuring luciferase activity in cells expressing GLP1R and CRE-luc. Results are presented as mean±standard error of the mean of at least three independent experiments.

    Article Snippet: Stability of GLP-1 analogs To evaluate the stability of GLP-1 and its analogs against DPP-IV activity, peptides were incubated at an initial concentration of 100 nM in DMEM containing 10% FBS or in 100% FBS at 37℃ for 0, 12, 24, 48, 72, and 96 hours in the presence or absence of 0.2 mM DPP-IV inhibitor diprotin A (Sigma-Aldrich, St Louis, MO, USA).

    Techniques: Activity Assay, Incubation, Concentration Assay, Modification, Luciferase, Expressing

    Potency of glucagon-like peptide-1 (GLP-1) analogs toward GLP-1 receptor (GLP1R). Ligand potencies of GLP-1 analogs were examined using HEK293T cells expressing GLP1R. Cells were treated with increasing concentrations of GLP-1 analogs for 6 hours, and luciferase activity was measured. The data on the sigmoidal curves and EC 50 values are presented as means±standard error of the mean of at least three independent experiments. CRE-luc, cAMP response element-luciferase; xGLP, Xenopus GLP-1.

    Journal: Endocrinology and Metabolism

    Article Title: A Novel Long-Acting Glucagon-Like Peptide-1 Agonist with Improved Efficacy in Insulin Secretion and β-Cell Growth

    doi: 10.3803/EnM.2014.29.3.320

    Figure Lengend Snippet: Potency of glucagon-like peptide-1 (GLP-1) analogs toward GLP-1 receptor (GLP1R). Ligand potencies of GLP-1 analogs were examined using HEK293T cells expressing GLP1R. Cells were treated with increasing concentrations of GLP-1 analogs for 6 hours, and luciferase activity was measured. The data on the sigmoidal curves and EC 50 values are presented as means±standard error of the mean of at least three independent experiments. CRE-luc, cAMP response element-luciferase; xGLP, Xenopus GLP-1.

    Article Snippet: Stability of GLP-1 analogs To evaluate the stability of GLP-1 and its analogs against DPP-IV activity, peptides were incubated at an initial concentration of 100 nM in DMEM containing 10% FBS or in 100% FBS at 37℃ for 0, 12, 24, 48, 72, and 96 hours in the presence or absence of 0.2 mM DPP-IV inhibitor diprotin A (Sigma-Aldrich, St Louis, MO, USA).

    Techniques: Expressing, Luciferase, Activity Assay

    Induction of β-cell growth by (xGLP-E4). INS-1 cells were seeded in 12-well plates at a density of 4×10 4 cells/well. Every 2 days, the respective peptides were added to the cultures in fresh medium containing the appropriate concentration of glucose. (A) Six and (B) 10 days after cell seeding, cells were washed, harvested, and counted. Results are presented as mean±standard error of the mean of at least three independent experiments. GLP-1, glucagon-like peptide-1; Exe-4, exendin-4; xGLP, Xenopus GLP-1. a vs. control (CTL) ( P

    Journal: Endocrinology and Metabolism

    Article Title: A Novel Long-Acting Glucagon-Like Peptide-1 Agonist with Improved Efficacy in Insulin Secretion and β-Cell Growth

    doi: 10.3803/EnM.2014.29.3.320

    Figure Lengend Snippet: Induction of β-cell growth by (xGLP-E4). INS-1 cells were seeded in 12-well plates at a density of 4×10 4 cells/well. Every 2 days, the respective peptides were added to the cultures in fresh medium containing the appropriate concentration of glucose. (A) Six and (B) 10 days after cell seeding, cells were washed, harvested, and counted. Results are presented as mean±standard error of the mean of at least three independent experiments. GLP-1, glucagon-like peptide-1; Exe-4, exendin-4; xGLP, Xenopus GLP-1. a vs. control (CTL) ( P

    Article Snippet: Stability of GLP-1 analogs To evaluate the stability of GLP-1 and its analogs against DPP-IV activity, peptides were incubated at an initial concentration of 100 nM in DMEM containing 10% FBS or in 100% FBS at 37℃ for 0, 12, 24, 48, 72, and 96 hours in the presence or absence of 0.2 mM DPP-IV inhibitor diprotin A (Sigma-Aldrich, St Louis, MO, USA).

    Techniques: Concentration Assay, CTL Assay

    Plasma glucose and hormones. Mean ± SEM concentrations of plasma glucose ( A ), serum insulin ( B ), serum C-peptide ( C ), plasma total GLP-1 ( D ), plasma total ghrelin ( E ), and serum leptin ( F ) assessed before (averaged across the 0915- and 0930-h baseline values) and after intranasal administration (vertical dotted line) of oxytocin (24 IU; ● and solid lines) and placebo (vehicle; ○ and dotted lines). Subjects ate from a test breakfast from 1000 to 1030 h and ingested snacks under the pretext of a taste test from 1240 to 1250 h. Mean baseline values of both conditions are averaged to a common baseline ( n = 20). * P

    Journal: Diabetes

    Article Title: Oxytocin Reduces Reward-Driven Food Intake in Humans

    doi: 10.2337/db13-0663

    Figure Lengend Snippet: Plasma glucose and hormones. Mean ± SEM concentrations of plasma glucose ( A ), serum insulin ( B ), serum C-peptide ( C ), plasma total GLP-1 ( D ), plasma total ghrelin ( E ), and serum leptin ( F ) assessed before (averaged across the 0915- and 0930-h baseline values) and after intranasal administration (vertical dotted line) of oxytocin (24 IU; ● and solid lines) and placebo (vehicle; ○ and dotted lines). Subjects ate from a test breakfast from 1000 to 1030 h and ingested snacks under the pretext of a taste test from 1240 to 1250 h. Mean baseline values of both conditions are averaged to a common baseline ( n = 20). * P

    Article Snippet: Circulating concentrations of growth hormone and active GLP-1 (i.e., the intact form of GLP-1) were likewise comparable between conditions (all P > 0.14).

    Techniques:

    Change of acetaminophen (A) and glucagon-like peptide 1 (GLP-1) (B) levels during the single 600-kcal solid (square) and liquid (circle) meals before (open symbols) and after (closed symbols) gastric bypass surgery. Meal was given at 0 minutes. Data are mean ± SEM; with repeated measure (A and B) * P

    Journal: Surgery for obesity and related diseases : official journal of the American Society for Bariatric Surgery

    Article Title: Effect of meal size and texture on gastric pouch emptying and glucagon-like peptide 1 after gastric bypass surgery

    doi: 10.1016/j.soard.2017.09.004

    Figure Lengend Snippet: Change of acetaminophen (A) and glucagon-like peptide 1 (GLP-1) (B) levels during the single 600-kcal solid (square) and liquid (circle) meals before (open symbols) and after (closed symbols) gastric bypass surgery. Meal was given at 0 minutes. Data are mean ± SEM; with repeated measure (A and B) * P

    Article Snippet: Insulin and total GLP-1 were measured by radioimmuno-assay (Millipore).

    Techniques:

    Dexamethasone reduces Cftr channel activity in FDLE cells. Graphs represent the mean + SEM of I SC in response to 100 nM dexamethasone for 24, 48 and 72 h measured in Ussing chambers. A: Forskolin-induced I SC (n = 25–56, *** p

    Journal: PLoS ONE

    Article Title: Glucocorticoids Distinctively Modulate the CFTR Channel with Possible Implications in Lung Development and Transition into Extrauterine Life

    doi: 10.1371/journal.pone.0124833

    Figure Lengend Snippet: Dexamethasone reduces Cftr channel activity in FDLE cells. Graphs represent the mean + SEM of I SC in response to 100 nM dexamethasone for 24, 48 and 72 h measured in Ussing chambers. A: Forskolin-induced I SC (n = 25–56, *** p

    Article Snippet: Forskolin (10 μM, # F-6886, Sigma-Aldrich) was added to the apical compartment to increase the intracellular cAMP concentration and thereby activate cAMP-sensitive ion channels like CFTR.

    Techniques: Activity Assay

    LY-294002 prevents the increase of CFTR activity induced by dexamethasone in Calu-3 cells. Graphs represent the mean + SEM of I SC in response to 100 nM dexamethasone and LY-294002 for 24 h measured in Ussing chambers. A: Forskolin-induced I SC (n = 12–18, ** p

    Journal: PLoS ONE

    Article Title: Glucocorticoids Distinctively Modulate the CFTR Channel with Possible Implications in Lung Development and Transition into Extrauterine Life

    doi: 10.1371/journal.pone.0124833

    Figure Lengend Snippet: LY-294002 prevents the increase of CFTR activity induced by dexamethasone in Calu-3 cells. Graphs represent the mean + SEM of I SC in response to 100 nM dexamethasone and LY-294002 for 24 h measured in Ussing chambers. A: Forskolin-induced I SC (n = 12–18, ** p

    Article Snippet: Forskolin (10 μM, # F-6886, Sigma-Aldrich) was added to the apical compartment to increase the intracellular cAMP concentration and thereby activate cAMP-sensitive ion channels like CFTR.

    Techniques: Activity Assay

    Effects of STAT3 inhibitor on sorafenib- and sunitinib-induced cell growth inhibition. After pretreatment with Stattic (STAT3 inhibitor, 10 µM) or DMSO (solvent) for 20 min, HaCaT, Caki-1, and HepG2 cells were incubated in a medium that included sorafenib or sunitinib at the indicated concentrations for 48 h. Cell viability was determined by WST-8 colorimetric assay. *p

    Journal: PLoS ONE

    Article Title: Association of Toxicity of Sorafenib and Sunitinib for Human Keratinocytes with Inhibition of Signal Transduction and Activator of Transcription 3 (STAT3)

    doi: 10.1371/journal.pone.0102110

    Figure Lengend Snippet: Effects of STAT3 inhibitor on sorafenib- and sunitinib-induced cell growth inhibition. After pretreatment with Stattic (STAT3 inhibitor, 10 µM) or DMSO (solvent) for 20 min, HaCaT, Caki-1, and HepG2 cells were incubated in a medium that included sorafenib or sunitinib at the indicated concentrations for 48 h. Cell viability was determined by WST-8 colorimetric assay. *p

    Article Snippet: Sunitinib malate and Hoechst 33258 were purchased from Sigma-Aldrich Chemical, Co. (St Louis, MO, US).

    Techniques: Inhibition, Incubation, Colorimetric Assay

    Effects of sorafenib and sunitinib on MAPK activation in HaCaT cells and effects of MAPK inhibitor on sorafenib and sunitinib-induced cell growth inhibition and signal transduction. (A) Alterations in MAPKs signal transduction. HaCaT cells were incubated in medium containing 1 µM sorafenib or sunitinib for the indicated times, followed by Western blot analysis using total cell lysates. (B) Effects of MAPK inhibitor on sorafenib- and sunitinib-induced cell growth inhibition. HaCaT cells were incubated with medium containing sorafenib at the indicated concentrations for 48 h after pretreatment with either U0126 (MEK1/2 inhibitor, 10 µM), SB203580 (p38 MAPK inhibitor, 10 µM), or DMSO (solvent) for 2 h. Cell viability was determined by WST-8 colorimetric assay. *p

    Journal: PLoS ONE

    Article Title: Association of Toxicity of Sorafenib and Sunitinib for Human Keratinocytes with Inhibition of Signal Transduction and Activator of Transcription 3 (STAT3)

    doi: 10.1371/journal.pone.0102110

    Figure Lengend Snippet: Effects of sorafenib and sunitinib on MAPK activation in HaCaT cells and effects of MAPK inhibitor on sorafenib and sunitinib-induced cell growth inhibition and signal transduction. (A) Alterations in MAPKs signal transduction. HaCaT cells were incubated in medium containing 1 µM sorafenib or sunitinib for the indicated times, followed by Western blot analysis using total cell lysates. (B) Effects of MAPK inhibitor on sorafenib- and sunitinib-induced cell growth inhibition. HaCaT cells were incubated with medium containing sorafenib at the indicated concentrations for 48 h after pretreatment with either U0126 (MEK1/2 inhibitor, 10 µM), SB203580 (p38 MAPK inhibitor, 10 µM), or DMSO (solvent) for 2 h. Cell viability was determined by WST-8 colorimetric assay. *p

    Article Snippet: Sunitinib malate and Hoechst 33258 were purchased from Sigma-Aldrich Chemical, Co. (St Louis, MO, US).

    Techniques: Activation Assay, Inhibition, Transduction, Incubation, Western Blot, Colorimetric Assay

    Effects of sorafenib and sunitinib on cellular apoptosis and the expression of apoptosis suppressors. (A) After pretreatment with 10 µM Stattic or DMSO for 20 min, HaCaT cells were incubated in medium containing sorafenib or sunitinib at the indicated concentrations for 24 h. Apoptotic cells were detected using FITC-labeled Annexin V/PI staining with the IN Cell Analyzer 2000. *p

    Journal: PLoS ONE

    Article Title: Association of Toxicity of Sorafenib and Sunitinib for Human Keratinocytes with Inhibition of Signal Transduction and Activator of Transcription 3 (STAT3)

    doi: 10.1371/journal.pone.0102110

    Figure Lengend Snippet: Effects of sorafenib and sunitinib on cellular apoptosis and the expression of apoptosis suppressors. (A) After pretreatment with 10 µM Stattic or DMSO for 20 min, HaCaT cells were incubated in medium containing sorafenib or sunitinib at the indicated concentrations for 24 h. Apoptotic cells were detected using FITC-labeled Annexin V/PI staining with the IN Cell Analyzer 2000. *p

    Article Snippet: Sunitinib malate and Hoechst 33258 were purchased from Sigma-Aldrich Chemical, Co. (St Louis, MO, US).

    Techniques: Expressing, Incubation, Labeling, Staining

    Signal transduction alterations involved in STAT3 after treatment with sorafenib and sunitinib and effects of STAT3C on cytotoxicity of sorafenib and sunitinib. (A) HaCaT, Caki-1, and HepG2 cells were incubated with a medium that included sorafenib or sunitinib at the indicated concentrations for 2 h. Thereafter, Western blot analysis was performed using total cell lysates. (B) Immunostaining images. HaCaT cells were treated with sorafenib (10 µM), sunitinib (10 µM), or DMSO (Control; cont) for 2 h and were fixed and incubated with an anti-STAT3 antibody, followed by incubation with FITC-conjugated anti-rabbit IgG (green), and then visualized with the IN Cell Analyzer 2000. Nuclear translocation of STAT3 was determined with cell population analysis by determining the nucleus/cytoplasm intensity ratio of green fluorescence. Bar shows 50 µm. (C) Effects of STAT3C transfection on sorafenib- and sunitinib-induced cell growth inhibition. HaCaT cells transiently transfected with STAT3C or an empty vector were preincubated for 24 h, followed by incubation in medium containing sorafenib or sunitinib at the indicated concentrations for 48 h. Cell viability was determined by WST-8 colorimetric assay. *p

    Journal: PLoS ONE

    Article Title: Association of Toxicity of Sorafenib and Sunitinib for Human Keratinocytes with Inhibition of Signal Transduction and Activator of Transcription 3 (STAT3)

    doi: 10.1371/journal.pone.0102110

    Figure Lengend Snippet: Signal transduction alterations involved in STAT3 after treatment with sorafenib and sunitinib and effects of STAT3C on cytotoxicity of sorafenib and sunitinib. (A) HaCaT, Caki-1, and HepG2 cells were incubated with a medium that included sorafenib or sunitinib at the indicated concentrations for 2 h. Thereafter, Western blot analysis was performed using total cell lysates. (B) Immunostaining images. HaCaT cells were treated with sorafenib (10 µM), sunitinib (10 µM), or DMSO (Control; cont) for 2 h and were fixed and incubated with an anti-STAT3 antibody, followed by incubation with FITC-conjugated anti-rabbit IgG (green), and then visualized with the IN Cell Analyzer 2000. Nuclear translocation of STAT3 was determined with cell population analysis by determining the nucleus/cytoplasm intensity ratio of green fluorescence. Bar shows 50 µm. (C) Effects of STAT3C transfection on sorafenib- and sunitinib-induced cell growth inhibition. HaCaT cells transiently transfected with STAT3C or an empty vector were preincubated for 24 h, followed by incubation in medium containing sorafenib or sunitinib at the indicated concentrations for 48 h. Cell viability was determined by WST-8 colorimetric assay. *p

    Article Snippet: Sunitinib malate and Hoechst 33258 were purchased from Sigma-Aldrich Chemical, Co. (St Louis, MO, US).

    Techniques: Transduction, Incubation, Western Blot, Immunostaining, Translocation Assay, Fluorescence, Transfection, Inhibition, Plasmid Preparation, Colorimetric Assay

    Chemical structures of sorafenib and sunitinib.

    Journal: PLoS ONE

    Article Title: Association of Toxicity of Sorafenib and Sunitinib for Human Keratinocytes with Inhibition of Signal Transduction and Activator of Transcription 3 (STAT3)

    doi: 10.1371/journal.pone.0102110

    Figure Lengend Snippet: Chemical structures of sorafenib and sunitinib.

    Article Snippet: Sunitinib malate and Hoechst 33258 were purchased from Sigma-Aldrich Chemical, Co. (St Louis, MO, US).

    Techniques:

    Differential endocytosis of primary and secondary CPP in human monocyte-derived macrophages and aortic endothelial cells. Human monocyte-derived macrophages (hMDM) or human aortic endothelial cells (haEC) were treated with Alexa488-labeled primary or secondary CPP, both 100 μg/mL calcium content, synthesized from FCS/DMEM supplemented with phosphate (final conc. 3.5 mM). Monomeric labeled-fetuin-A was included as a comparator (1 mg/ml). Cell-associated fluorescence was measured by flow cytometry in fixed cells at the stated timepoints.  (A)  Endocyosis observed in hMDM and  (B)  haEC, respectively.  (C,D)  hMDM were pre-treated for 30–60 min with one of several inhibitors (vs. vehicle) or receptor blocking antibodies (vs. isotype control).  (C)  Pre-treatment with cytochalasin  (D) , an inhibitor of actin polymerization, markedly attenuated uptake of both primary and secondary CPP and (

    Journal: Frontiers in Immunology

    Article Title: Cellular Clearance and Biological Activity of Calciprotein Particles Depend on Their Maturation State and Crystallinity

    doi: 10.3389/fimmu.2018.01991

    Figure Lengend Snippet: Differential endocytosis of primary and secondary CPP in human monocyte-derived macrophages and aortic endothelial cells. Human monocyte-derived macrophages (hMDM) or human aortic endothelial cells (haEC) were treated with Alexa488-labeled primary or secondary CPP, both 100 μg/mL calcium content, synthesized from FCS/DMEM supplemented with phosphate (final conc. 3.5 mM). Monomeric labeled-fetuin-A was included as a comparator (1 mg/ml). Cell-associated fluorescence was measured by flow cytometry in fixed cells at the stated timepoints. (A) Endocyosis observed in hMDM and (B) haEC, respectively. (C,D) hMDM were pre-treated for 30–60 min with one of several inhibitors (vs. vehicle) or receptor blocking antibodies (vs. isotype control). (C) Pre-treatment with cytochalasin (D) , an inhibitor of actin polymerization, markedly attenuated uptake of both primary and secondary CPP and (

    Article Snippet: While in others, cells were pre-treated for 30 min with one of several chemical inhibitors at previously defined concentrations as detailed in Supplementary Table (all from Sigma): cytochalasin D (10 μM), chlorpromazine (10 μg/ml), filipin (2 μg/ml), genestein (200 μM), 5-(N,N-dimethyl)amiloride hydrochloride (10 μM; 5-DMA), MβCD (2 mM), Ly294002 (100 μM), monodansylcadaverine (100 μM; MDC) polyinosinic acid (10 μg/ml) or vehicle (DMSO/media); then washed and switched to media containing AF488-CPP (100 μg/ml) and incubated for 60 min at 37°C; or were pre-treated with blocking antibodies (60 min at 4°C) directed against SRA (AbD Serotec, 10 μg/ml;), TLR2/4/6 (10 μg/ml; Invivogen), CD36 (clone FA6-152; 10 μg/ml; Hycult Biotech), or annexin-2 (20 μg/ml; BD Biosciences) or relevant isotype IgG control (all 10 μg/ml; eBioscience) before incubation with CPP-containing media for 60 min at 37°C.

    Techniques: Conditioned Place Preference, Derivative Assay, Labeling, Synthesized, Fluorescence, Flow Cytometry, Cytometry, Blocking Assay

    Benzimidazolones enhance benzamil-sensitive I SC . Effects of DC-EBIO (0.3 mM) on benzamil- and EIPA-sensitive I SC of rat FDLE cell monolayers. The figure shows the current reduction caused by 10 μM benzamil and 100 μM EIPA. Mean treatment-associated changes in current ± SEM of 12 (control/benzamil), 9 (DC-EBIO/benzamil), 11 (control/EIPA) and 14 (DC-EBIO/EIPA) monolayers (* P

    Journal: British Journal of Pharmacology

    Article Title: Benzimidazolones enhance the function of epithelial Na+ transport

    doi: 10.1111/bph.12027

    Figure Lengend Snippet: Benzimidazolones enhance benzamil-sensitive I SC . Effects of DC-EBIO (0.3 mM) on benzamil- and EIPA-sensitive I SC of rat FDLE cell monolayers. The figure shows the current reduction caused by 10 μM benzamil and 100 μM EIPA. Mean treatment-associated changes in current ± SEM of 12 (control/benzamil), 9 (DC-EBIO/benzamil), 11 (control/EIPA) and 14 (DC-EBIO/EIPA) monolayers (* P

    Article Snippet: Amiloride (A-7410, Sigma, Chemical Company, St. Louis, MO), amphotericin B (A-4888, Sigma), benzamil (B-2417, Sigma), CFTRinh 172 (3430, Tocris, Bristol, UK), chlorzoxazone (C-4397, Sigma), DC-EBIO (5,6-dichloro-1-ethyl-1,3-dihydro-2-benzimidazolone) (1422, Tocris), 1-EBIO (1-ethyl-1,3-dihydro-2-benzimidazolone) (SML0034, Sigma), EIPA (5-( N -Ethyl- N -isopropyl)amiloride) (A-3085, Sigma), N -methyl- d -glucamine (NMDG+ , M-2004, Sigma), ouabain (O-3125, Sigma).

    Techniques:

    Amiloride and EIPA induced a decrease in [Na + ] i in non-capacitated sperm. Sperm from the cauda epididymus were placed in medium that did not support capacitation, loaded with CoroNa Red, washed and incubated for an additional 60 minutes in the absence

    Journal: Journal of Cell Science

    Article Title: Flow cytometry analysis reveals a decrease in intracellular sodium during sperm capacitation

    doi: 10.1242/jcs.093344

    Figure Lengend Snippet: Amiloride and EIPA induced a decrease in [Na + ] i in non-capacitated sperm. Sperm from the cauda epididymus were placed in medium that did not support capacitation, loaded with CoroNa Red, washed and incubated for an additional 60 minutes in the absence

    Article Snippet: Chemicals were obtained from the following sources: bovine serum albumin (BSA; fatty acid-free), dibutyryl-cAMP (Bt2cAMP), 3-isobutyl-1-methylxanthine (IBMX), 4-bromo-calcium ionophore A23187, amiloride hydrochloride hydrate, 5-( N -ethyl- N -isopropyl) amiloride (EIPA), sodium gluconate and carbonyl cyanide m -chlorophenylhydrazone (CCCP) were from Sigma (St Louis, MO); H-89 was from Cayman Chemical Company (Ann Arbor, MI); rabbit monoclonal anti-phosphorylated PKA substrate (clone 100G7E) was purchased from Cell Signaling (Danvers, MA); anti-phosphorylated tyrosine (Y- P ) monoclonal antibody (clone 4G10) was from Upstate Biotechnology (Lake Placid, NY); horseradish peroxidase-conjugated anti-mouse and anti-rabbit IgG were purchased from Jackson ImmunoResearch Laboratories (West Grove, PA) and GE Life Sciences, respectively; propidium iodide and CoroNaRed sodium fluorescent probes were from Invitrogen.

    Techniques: Incubation

    Fig. 3. Activation of mesangial cell ERK, JNK/SAPK and p38 MAPK pathways by ET-1. ( A ) Cells were treated with the indicated agonists for the indicated times. Crude extracts were prepared and subjected to SDS–PAGE and immunoblotting with phospho-MAPK-specific antibodies (top three panels) or total MAPK blots (bottom three panels). ( B ) The same as (A), except that cells were treated for longer time periods. ( C ) Activation of MKKs by ET-1. Cells were treated with the indicated stimuli as in (A). Extracts were prepared and subjected to SDS–PAGE and immunoblotting with the indicated phospho-MKK-specific antibodies (top three panels) or, as a loading control, with an antibody to smooth muscle actin (SMA, bottom panel). ( D ) The same as (C), except that blots were probed with anti-phospho-MEK only. ( E ) Activation of CREB by ET-1. Mesangial cells were treated with the indicated agonists for the indicated times, and crude extracts were subjected to SDS–PAGE and immunoblotting with phospho-CREB/ATF-1-specific antibody.

    Journal: The EMBO Journal

    Article Title: Signaling pathways and late-onset gene induction associated with renal mesangial cell hypertrophy

    doi: 10.1093/emboj/cdf535

    Figure Lengend Snippet: Fig. 3. Activation of mesangial cell ERK, JNK/SAPK and p38 MAPK pathways by ET-1. ( A ) Cells were treated with the indicated agonists for the indicated times. Crude extracts were prepared and subjected to SDS–PAGE and immunoblotting with phospho-MAPK-specific antibodies (top three panels) or total MAPK blots (bottom three panels). ( B ) The same as (A), except that cells were treated for longer time periods. ( C ) Activation of MKKs by ET-1. Cells were treated with the indicated stimuli as in (A). Extracts were prepared and subjected to SDS–PAGE and immunoblotting with the indicated phospho-MKK-specific antibodies (top three panels) or, as a loading control, with an antibody to smooth muscle actin (SMA, bottom panel). ( D ) The same as (C), except that blots were probed with anti-phospho-MEK only. ( E ) Activation of CREB by ET-1. Mesangial cells were treated with the indicated agonists for the indicated times, and crude extracts were subjected to SDS–PAGE and immunoblotting with phospho-CREB/ATF-1-specific antibody.

    Article Snippet: We used rabbit anti-total ERK (Santa Cruz Biotechnology), JNK/SAPK (Cell Signaling Technology), p38 (Santa Cruz Biotechnology), PKB (Cell Signaling Technology) and anti-SMA (Sigma).

    Techniques: Activation Assay, SDS Page

    Schematic diagram of ( A ) cRGD-conjugated PAMAMG2-g-cyclodextrin (CDG2-cRGD) and ( B ) self-assembly of pDNA/CDG2-cRGD polyplex. Abbreviations: CD, cyclodextrin; CDG2, PAMAMG2-g-cyclodextrin; CDI, 1,1′-carbonyldiimidazole; cRGD, cyclic arginylglycylaspartic acid peptide; DMSO, dimethyl sulfoxide; PAMAMG2, Generation 2 polyamidoamine; PBS, phosphate-buffered saline; pDNA, plasmid DNA; pVEGF, plasmid vector with the VEGF gene; SPDP, 3-(2-pyridyldithio)propionic acid N -hydroxysuccinimide ester; VEGF, vascular endothelial growth factor.

    Journal: International Journal of Nanomedicine

    Article Title: Endothelial cell-targeted pVEGF165 polyplex plays a pivotal role in inhibiting intimal thickening after vascular injury

    doi: 10.2147/IJN.S88109

    Figure Lengend Snippet: Schematic diagram of ( A ) cRGD-conjugated PAMAMG2-g-cyclodextrin (CDG2-cRGD) and ( B ) self-assembly of pDNA/CDG2-cRGD polyplex. Abbreviations: CD, cyclodextrin; CDG2, PAMAMG2-g-cyclodextrin; CDI, 1,1′-carbonyldiimidazole; cRGD, cyclic arginylglycylaspartic acid peptide; DMSO, dimethyl sulfoxide; PAMAMG2, Generation 2 polyamidoamine; PBS, phosphate-buffered saline; pDNA, plasmid DNA; pVEGF, plasmid vector with the VEGF gene; SPDP, 3-(2-pyridyldithio)propionic acid N -hydroxysuccinimide ester; VEGF, vascular endothelial growth factor.

    Article Snippet: 1,1′-Carbonyldiimidazole (CDI) and dithiothreitol (DTT) were obtained from Sigma-Aldrich. cRGD (molecular weight =580 Da) was synthesized by GL Biochem Company (Shanghai, People’s Republic of China).

    Techniques: Plasmid Preparation

    Insulin induces phosphorylation of rNCC at threonine 58 (A and B) Representative Western blot assays performed using anti Flag monoclonal antibody, the pNCC-T58 phosphoantibody, and β-actin antibody, as stated. Total proteins were extracted from Xenopus laevis oocytes injected with water or rNCC cRNA and exposed to insulin for 0, 5, 15 and 30 minutes (A), or to 15 minutes in the absence or presence of wortmannin (B) (C) A representative Western blot analysis of protein extracts from ex-vivo perfused rat-kidneys. The kidneys were perfused with isotonic control solution or with isotonic solution containing 0.250 U/mL of insulin. (D) Densitometry analysis of phosphor-NCC over total rNCC from three different Western blot analyses compiling a total of 10 controls and 16 insulin perfused kidneys. (*p

    Journal: Journal of hypertension

    Article Title: Insulin Increases the Functional Activity of the Renal NaCl cotransporter

    doi: 10.1097/HJH.0b013e32835bbb83

    Figure Lengend Snippet: Insulin induces phosphorylation of rNCC at threonine 58 (A and B) Representative Western blot assays performed using anti Flag monoclonal antibody, the pNCC-T58 phosphoantibody, and β-actin antibody, as stated. Total proteins were extracted from Xenopus laevis oocytes injected with water or rNCC cRNA and exposed to insulin for 0, 5, 15 and 30 minutes (A), or to 15 minutes in the absence or presence of wortmannin (B) (C) A representative Western blot analysis of protein extracts from ex-vivo perfused rat-kidneys. The kidneys were perfused with isotonic control solution or with isotonic solution containing 0.250 U/mL of insulin. (D) Densitometry analysis of phosphor-NCC over total rNCC from three different Western blot analyses compiling a total of 10 controls and 16 insulin perfused kidneys. (*p

    Article Snippet: Insulin alone or in the presence of inhibitors of the insulin transduction pathways: Wortmannin (Sigma), Rapamycin, UO126 (Callbiochem) [ ], SB203580 (Callbiochem), AKT IV Inhibitor (Callbiochem), AZD8055 (Selleck) [ ] were dissolved at 10mM dimethylsulfoxido (DMSO) as stock solutions and preserved at −20°C.

    Techniques: Western Blot, Injection, Ex Vivo

    Insulin activates NCC cotransporter through a PI3K-mTORC2-AKT pathway Xenopus laevis oocytes were injected with rNCC cRNA and three days later Na + uptake was assessed by incubated the oocytes in the absence ( white bars ) or presence ( black bars ) of insulin, in the absence or presence of the PI3K inhibitor wortmannin (A), the specific inhibitor of mTORc2, AZD8055 (B); or in the presence of the AKT Inhibitor, AKT IV-I (C). or the inhibitors of MAP kinases. The mean value of the cotransporter without insulin was taken as 1 and the effect of insulin was normalized to that value (*p

    Journal: Journal of hypertension

    Article Title: Insulin Increases the Functional Activity of the Renal NaCl cotransporter

    doi: 10.1097/HJH.0b013e32835bbb83

    Figure Lengend Snippet: Insulin activates NCC cotransporter through a PI3K-mTORC2-AKT pathway Xenopus laevis oocytes were injected with rNCC cRNA and three days later Na + uptake was assessed by incubated the oocytes in the absence ( white bars ) or presence ( black bars ) of insulin, in the absence or presence of the PI3K inhibitor wortmannin (A), the specific inhibitor of mTORc2, AZD8055 (B); or in the presence of the AKT Inhibitor, AKT IV-I (C). or the inhibitors of MAP kinases. The mean value of the cotransporter without insulin was taken as 1 and the effect of insulin was normalized to that value (*p

    Article Snippet: Insulin alone or in the presence of inhibitors of the insulin transduction pathways: Wortmannin (Sigma), Rapamycin, UO126 (Callbiochem) [ ], SB203580 (Callbiochem), AKT IV Inhibitor (Callbiochem), AZD8055 (Selleck) [ ] were dissolved at 10mM dimethylsulfoxido (DMSO) as stock solutions and preserved at −20°C.

    Techniques: Injection, Incubation

    AP301 activation of ENaC requires pore-forming α - or δ -subunit coexpression. (A) Amiloride (10 µ M)-sensitive current in different subunits and subunit combinations expressed in HEK-293 cells. Cells were patched in the whole-cell

    Journal: Molecular Pharmacology

    Article Title: Mechanism of Action of Novel Lung Edema Therapeutic AP301 by Activation of the Epithelial Sodium Channel

    doi: 10.1124/mol.113.089409

    Figure Lengend Snippet: AP301 activation of ENaC requires pore-forming α - or δ -subunit coexpression. (A) Amiloride (10 µ M)-sensitive current in different subunits and subunit combinations expressed in HEK-293 cells. Cells were patched in the whole-cell

    Article Snippet: Both amiloride hydrochloride hydrate and TEA were purchased from Sigma-Aldrich GmbH.

    Techniques: Activation Assay

    Amiloride-sensitive AP301-induced current activation in transiently and endogenously expressed ENaC. (A) AP301 activated the Na + current in transiently expressed αβγ -hENaC in HEK-293 and CHO cells with similar efficacy and potency

    Journal: Molecular Pharmacology

    Article Title: Mechanism of Action of Novel Lung Edema Therapeutic AP301 by Activation of the Epithelial Sodium Channel

    doi: 10.1124/mol.113.089409

    Figure Lengend Snippet: Amiloride-sensitive AP301-induced current activation in transiently and endogenously expressed ENaC. (A) AP301 activated the Na + current in transiently expressed αβγ -hENaC in HEK-293 and CHO cells with similar efficacy and potency

    Article Snippet: Both amiloride hydrochloride hydrate and TEA were purchased from Sigma-Aldrich GmbH.

    Techniques: Activation Assay

    AP301 selectively activates αβγ -hENaC expressed in HEK-293 cells. (A) Mean values of inward currents during the control phase, after sequential addition of l - cis (300 µ M), AP301 (120 nM) and final addition of amiloride

    Journal: Molecular Pharmacology

    Article Title: Mechanism of Action of Novel Lung Edema Therapeutic AP301 by Activation of the Epithelial Sodium Channel

    doi: 10.1124/mol.113.089409

    Figure Lengend Snippet: AP301 selectively activates αβγ -hENaC expressed in HEK-293 cells. (A) Mean values of inward currents during the control phase, after sequential addition of l - cis (300 µ M), AP301 (120 nM) and final addition of amiloride

    Article Snippet: Both amiloride hydrochloride hydrate and TEA were purchased from Sigma-Aldrich GmbH.

    Techniques:

    AP301 induced amiloride-sensitive current in proteolytically cleaved hENaC. (A) Cells were patched in the whole-cell mode; inward current was elicited at −100 mV. AP301 (up to 200 nM) did not induce inward sodium current from hENaC endogenously

    Journal: Molecular Pharmacology

    Article Title: Mechanism of Action of Novel Lung Edema Therapeutic AP301 by Activation of the Epithelial Sodium Channel

    doi: 10.1124/mol.113.089409

    Figure Lengend Snippet: AP301 induced amiloride-sensitive current in proteolytically cleaved hENaC. (A) Cells were patched in the whole-cell mode; inward current was elicited at −100 mV. AP301 (up to 200 nM) did not induce inward sodium current from hENaC endogenously

    Article Snippet: Both amiloride hydrochloride hydrate and TEA were purchased from Sigma-Aldrich GmbH.

    Techniques:

    Molecular docking of analogues 1 and 32 within the active site of cathepsin L. (A) Analogue 1 and (B) analogue 32 are shown in ball and stick mode (C, gray; H, white; O, red; N, blue; S, yellow; F, light blue; Br, brown). Cathepsin L is shown in ribbon

    Journal: Bioorganic & medicinal chemistry

    Article Title: Synthesis and biochemical evaluation of benzoylbenzophenone thiosemicarbazone analogues as potent and selective inhibitors of cathepsin L

    doi: 10.1016/j.bmc.2015.09.036

    Figure Lengend Snippet: Molecular docking of analogues 1 and 32 within the active site of cathepsin L. (A) Analogue 1 and (B) analogue 32 are shown in ball and stick mode (C, gray; H, white; O, red; N, blue; S, yellow; F, light blue; Br, brown). Cathepsin L is shown in ribbon

    Article Snippet: The final assay conditions were 100 mM NaOAc buffer, pH 5.5, 1 mM EDTA, 3 mM DTT, 0.01% Brij 35 (Sigma), 1 nM cathepsin L, 2% DMSO (Sigma) and 50 μM of Z-FR-AMC, in a total reaction volume of 200 μl.

    Techniques:

    Activity of selected benzophenone thiosemicarbazone analogues against cathepsin L

    Journal: Bioorganic & medicinal chemistry

    Article Title: Synthesis and biochemical evaluation of benzoylbenzophenone thiosemicarbazone analogues as potent and selective inhibitors of cathepsin L

    doi: 10.1016/j.bmc.2015.09.036

    Figure Lengend Snippet: Activity of selected benzophenone thiosemicarbazone analogues against cathepsin L

    Article Snippet: The final assay conditions were 100 mM NaOAc buffer, pH 5.5, 1 mM EDTA, 3 mM DTT, 0.01% Brij 35 (Sigma), 1 nM cathepsin L, 2% DMSO (Sigma) and 50 μM of Z-FR-AMC, in a total reaction volume of 200 μl.

    Techniques: Activity Assay

    Representative progress curves of cathepsin L (1 nM) activity using Z-FR-AMC (10 μM) as substrate and increasing concentrations of (A) inhibitor 1 and (B) inhibitor 32 (0–10 μM). Reactions were initiated by the addition of enzyme

    Journal: Bioorganic & medicinal chemistry

    Article Title: Synthesis and biochemical evaluation of benzoylbenzophenone thiosemicarbazone analogues as potent and selective inhibitors of cathepsin L

    doi: 10.1016/j.bmc.2015.09.036

    Figure Lengend Snippet: Representative progress curves of cathepsin L (1 nM) activity using Z-FR-AMC (10 μM) as substrate and increasing concentrations of (A) inhibitor 1 and (B) inhibitor 32 (0–10 μM). Reactions were initiated by the addition of enzyme

    Article Snippet: The final assay conditions were 100 mM NaOAc buffer, pH 5.5, 1 mM EDTA, 3 mM DTT, 0.01% Brij 35 (Sigma), 1 nM cathepsin L, 2% DMSO (Sigma) and 50 μM of Z-FR-AMC, in a total reaction volume of 200 μl.

    Techniques: Activity Assay

    Representative potent inhibitors of cathepsin L utilizing various warheads. Notable selective inhibitors of cathepsin L include the natural product gallinamide A I the epoxysuccinamide inhibitor Clik 148 II the oxocarbazate III the vinyl sulfonate

    Journal: Bioorganic & medicinal chemistry

    Article Title: Synthesis and biochemical evaluation of benzoylbenzophenone thiosemicarbazone analogues as potent and selective inhibitors of cathepsin L

    doi: 10.1016/j.bmc.2015.09.036

    Figure Lengend Snippet: Representative potent inhibitors of cathepsin L utilizing various warheads. Notable selective inhibitors of cathepsin L include the natural product gallinamide A I the epoxysuccinamide inhibitor Clik 148 II the oxocarbazate III the vinyl sulfonate

    Article Snippet: The final assay conditions were 100 mM NaOAc buffer, pH 5.5, 1 mM EDTA, 3 mM DTT, 0.01% Brij 35 (Sigma), 1 nM cathepsin L, 2% DMSO (Sigma) and 50 μM of Z-FR-AMC, in a total reaction volume of 200 μl.

    Techniques:

    Effect of cathepsin L inhibitors on tumor cell invasion. PC-3ML prostate cancer cell invasion in the presence of cathepsin inhibitors compound 1 (A) or compound 8 (B). Data are mean and standard error values of 3-5 independent experiments.

    Journal: Bioorganic & medicinal chemistry

    Article Title: Synthesis and biochemical evaluation of benzoylbenzophenone thiosemicarbazone analogues as potent and selective inhibitors of cathepsin L

    doi: 10.1016/j.bmc.2015.09.036

    Figure Lengend Snippet: Effect of cathepsin L inhibitors on tumor cell invasion. PC-3ML prostate cancer cell invasion in the presence of cathepsin inhibitors compound 1 (A) or compound 8 (B). Data are mean and standard error values of 3-5 independent experiments.

    Article Snippet: The final assay conditions were 100 mM NaOAc buffer, pH 5.5, 1 mM EDTA, 3 mM DTT, 0.01% Brij 35 (Sigma), 1 nM cathepsin L, 2% DMSO (Sigma) and 50 μM of Z-FR-AMC, in a total reaction volume of 200 μl.

    Techniques:

    Summary of the effects of R-009 on Calu-3WT and Calu-3KD epithelia and illustrated in . The filled columns show SCC inhibition caused by bumetanide (100 μ M added basolaterally) and acetazolamide (100 μ M added both

    Journal:

    Article Title: Chloride transporting capability of Calu-3 epithelia following persistent knockdown of the cystic fibrosis transmembrane conductance regulator, CFTR

    doi: 10.1038/sj.bjp.0707175

    Figure Lengend Snippet: Summary of the effects of R-009 on Calu-3WT and Calu-3KD epithelia and illustrated in . The filled columns show SCC inhibition caused by bumetanide (100 μ M added basolaterally) and acetazolamide (100 μ M added both

    Article Snippet: Sources of drugs used in the study were as follows: acetazolamide, amiloride, bumetanide, carbachol (CCh) and isobutylmethylxanthine were all from Sigma; forskolin was from Calbiochem; 5,6-dichloro-1-ethyl-1,3-dihydro-2H-benzimidazol-2-one (DCEBIO) was from Tocris; (–)-7-[(2R,4aR,5R,7aR0-2-(1,1-difluoropentyl)-2hydroxy-6oxooctahydrocyclopenta[b]pyran-5-yl]heptanoic acid (lubiprostone) (Amitiza) from Takeda Pharmaceuticals America Inc., IL, USA; polypeptide antibiotics from Dr OL Shotwell and N-(2-naphthalenyl)-[(3,5 dibromo-2,4-dihydroxy phenyl)methylene glycine hydrazide] (GlyH-101) from Professor AS Verkman.

    Techniques: Inhibition

    SCC records for three Calu-3WT epithelia from the same batch. The upper record ( a ) was used as a control for the solvents used for forskolin and GlyH-101; these did not affect the responsiveness to lubiprostone (Lubi) or to CCh and bumetanide. The middle

    Journal:

    Article Title: Chloride transporting capability of Calu-3 epithelia following persistent knockdown of the cystic fibrosis transmembrane conductance regulator, CFTR

    doi: 10.1038/sj.bjp.0707175

    Figure Lengend Snippet: SCC records for three Calu-3WT epithelia from the same batch. The upper record ( a ) was used as a control for the solvents used for forskolin and GlyH-101; these did not affect the responsiveness to lubiprostone (Lubi) or to CCh and bumetanide. The middle

    Article Snippet: Sources of drugs used in the study were as follows: acetazolamide, amiloride, bumetanide, carbachol (CCh) and isobutylmethylxanthine were all from Sigma; forskolin was from Calbiochem; 5,6-dichloro-1-ethyl-1,3-dihydro-2H-benzimidazol-2-one (DCEBIO) was from Tocris; (–)-7-[(2R,4aR,5R,7aR0-2-(1,1-difluoropentyl)-2hydroxy-6oxooctahydrocyclopenta[b]pyran-5-yl]heptanoic acid (lubiprostone) (Amitiza) from Takeda Pharmaceuticals America Inc., IL, USA; polypeptide antibiotics from Dr OL Shotwell and N-(2-naphthalenyl)-[(3,5 dibromo-2,4-dihydroxy phenyl)methylene glycine hydrazide] (GlyH-101) from Professor AS Verkman.

    Techniques:

    HDACis present cytotoxicity effect on canine B-cell lymphoma CLBL-1 cells (6 × 10 4 ) were subjected to the indicated concentrations of HDACis - CI-994, panobinostat, SBHA, SAHA, scriptaid, trichostatin A and tubacin ( A – G ). After 24 h treatment, cell viability and proliferation were evaluated with WST-1 reagent. Two replicate wells were used to determinate each data point and three independent experiments were carried out in different days. Best-fit IC50 values of each HDACis were calculated using the log (inhibitor) vs response (variable slope) function ( H ).

    Journal: Oncotarget

    Article Title: The histone deacetylase inhibitor panobinostat is a potent antitumor agent in canine diffuse large B-cell lymphoma

    doi: 10.18632/oncotarget.25580

    Figure Lengend Snippet: HDACis present cytotoxicity effect on canine B-cell lymphoma CLBL-1 cells (6 × 10 4 ) were subjected to the indicated concentrations of HDACis - CI-994, panobinostat, SBHA, SAHA, scriptaid, trichostatin A and tubacin ( A – G ). After 24 h treatment, cell viability and proliferation were evaluated with WST-1 reagent. Two replicate wells were used to determinate each data point and three independent experiments were carried out in different days. Best-fit IC50 values of each HDACis were calculated using the log (inhibitor) vs response (variable slope) function ( H ).

    Article Snippet: The Histone Deacetylase (HDAC) Inhibitor Set II, which includes CI-994, Panobinostat (LBH589), SAHA, SBHA, Scriptaid, Trichostatin A and Tubacin, was purchased from Sigma-Aldrich (St. Louis, MO, Cat # EPI009).

    Techniques: