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  • 99
    Thermo Fisher amicon ultra 4 centrifugal filter units
    Amicon Ultra 4 Centrifugal Filter Units, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore amicon ultra 4 centrifugal filter
    The specific blockade of TβRI kinase ablated Smad2 activation and dramatically affected the induction of the EMT markers, vimentin and MMPs by FK506. HK-2 cells were cultured on 6-well plates and treated with vehicle control, 5 µM FK506 with or without 500 nM of a TGFβRI/ALK5 kinase inhibitor V (SD-208). ( A ) Cells were incubated for 48 h with SD-208 concentrations ranging from 0–20μM. LDH and resazurin assays were conducted to assess SD-208 inhibitor toxicity; ( B ) Whole cell protein was extracted at 48 h with RIPA buffer. Equal amounts of protein were separated by SDS-PAGE electrophoresis, transferred to nitrocellulose and indirectly probed for vimentin and fibronectin using a mAb and ECL detection system. Whole Smad2 and GAPDH was used as a loading control. A representative blot is shown from three independent experiments. Densitometry is shown for the replicate blots; ( C ) Cell culture supernatants were harvested, centrifuged to remove cellular debris and concentrated using <t>Amicon</t> <t>Ultra-4</t> Centrifugal Filter Devices (Millipore). Gelatin substrate zymography was used to separate and assess MMP-9 and MMP-2 activity. Clear areas represent areas of gelatinolytic activity. A representative zymogram is shown from three independent experiments. ** and *** indicates different levels of significant compared to time-matched control; * , ** and *** indicates varying levels of significance compared to treatment + inhibitor.
    Amicon Ultra 4 Centrifugal Filter, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3643 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Merck KGaA amicon ultra 4 centrifugal filter
    The specific blockade of TβRI kinase ablated Smad2 activation and dramatically affected the induction of the EMT markers, vimentin and MMPs by FK506. HK-2 cells were cultured on 6-well plates and treated with vehicle control, 5 µM FK506 with or without 500 nM of a TGFβRI/ALK5 kinase inhibitor V (SD-208). ( A ) Cells were incubated for 48 h with SD-208 concentrations ranging from 0–20μM. LDH and resazurin assays were conducted to assess SD-208 inhibitor toxicity; ( B ) Whole cell protein was extracted at 48 h with RIPA buffer. Equal amounts of protein were separated by SDS-PAGE electrophoresis, transferred to nitrocellulose and indirectly probed for vimentin and fibronectin using a mAb and ECL detection system. Whole Smad2 and GAPDH was used as a loading control. A representative blot is shown from three independent experiments. Densitometry is shown for the replicate blots; ( C ) Cell culture supernatants were harvested, centrifuged to remove cellular debris and concentrated using <t>Amicon</t> <t>Ultra-4</t> Centrifugal Filter Devices (Millipore). Gelatin substrate zymography was used to separate and assess MMP-9 and MMP-2 activity. Clear areas represent areas of gelatinolytic activity. A representative zymogram is shown from three independent experiments. ** and *** indicates different levels of significant compared to time-matched control; * , ** and *** indicates varying levels of significance compared to treatment + inhibitor.
    Amicon Ultra 4 Centrifugal Filter, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 94/100, based on 696 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co amicon ultra 4 centrifugal filters
    The specific blockade of TβRI kinase ablated Smad2 activation and dramatically affected the induction of the EMT markers, vimentin and MMPs by FK506. HK-2 cells were cultured on 6-well plates and treated with vehicle control, 5 µM FK506 with or without 500 nM of a TGFβRI/ALK5 kinase inhibitor V (SD-208). ( A ) Cells were incubated for 48 h with SD-208 concentrations ranging from 0–20μM. LDH and resazurin assays were conducted to assess SD-208 inhibitor toxicity; ( B ) Whole cell protein was extracted at 48 h with RIPA buffer. Equal amounts of protein were separated by SDS-PAGE electrophoresis, transferred to nitrocellulose and indirectly probed for vimentin and fibronectin using a mAb and ECL detection system. Whole Smad2 and GAPDH was used as a loading control. A representative blot is shown from three independent experiments. Densitometry is shown for the replicate blots; ( C ) Cell culture supernatants were harvested, centrifuged to remove cellular debris and concentrated using <t>Amicon</t> <t>Ultra-4</t> Centrifugal Filter Devices (Millipore). Gelatin substrate zymography was used to separate and assess MMP-9 and MMP-2 activity. Clear areas represent areas of gelatinolytic activity. A representative zymogram is shown from three independent experiments. ** and *** indicates different levels of significant compared to time-matched control; * , ** and *** indicates varying levels of significance compared to treatment + inhibitor.
    Amicon Ultra 4 Centrifugal Filters, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Merck KGaA amicon ultra 4 10k centrifugal filter
    The specific blockade of TβRI kinase ablated Smad2 activation and dramatically affected the induction of the EMT markers, vimentin and MMPs by FK506. HK-2 cells were cultured on 6-well plates and treated with vehicle control, 5 µM FK506 with or without 500 nM of a TGFβRI/ALK5 kinase inhibitor V (SD-208). ( A ) Cells were incubated for 48 h with SD-208 concentrations ranging from 0–20μM. LDH and resazurin assays were conducted to assess SD-208 inhibitor toxicity; ( B ) Whole cell protein was extracted at 48 h with RIPA buffer. Equal amounts of protein were separated by SDS-PAGE electrophoresis, transferred to nitrocellulose and indirectly probed for vimentin and fibronectin using a mAb and ECL detection system. Whole Smad2 and GAPDH was used as a loading control. A representative blot is shown from three independent experiments. Densitometry is shown for the replicate blots; ( C ) Cell culture supernatants were harvested, centrifuged to remove cellular debris and concentrated using <t>Amicon</t> <t>Ultra-4</t> Centrifugal Filter Devices (Millipore). Gelatin substrate zymography was used to separate and assess MMP-9 and MMP-2 activity. Clear areas represent areas of gelatinolytic activity. A representative zymogram is shown from three independent experiments. ** and *** indicates different levels of significant compared to time-matched control; * , ** and *** indicates varying levels of significance compared to treatment + inhibitor.
    Amicon Ultra 4 10k Centrifugal Filter, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co amicon ultra 4 10k centrifugal filter
    The specific blockade of TβRI kinase ablated Smad2 activation and dramatically affected the induction of the EMT markers, vimentin and MMPs by FK506. HK-2 cells were cultured on 6-well plates and treated with vehicle control, 5 µM FK506 with or without 500 nM of a TGFβRI/ALK5 kinase inhibitor V (SD-208). ( A ) Cells were incubated for 48 h with SD-208 concentrations ranging from 0–20μM. LDH and resazurin assays were conducted to assess SD-208 inhibitor toxicity; ( B ) Whole cell protein was extracted at 48 h with RIPA buffer. Equal amounts of protein were separated by SDS-PAGE electrophoresis, transferred to nitrocellulose and indirectly probed for vimentin and fibronectin using a mAb and ECL detection system. Whole Smad2 and GAPDH was used as a loading control. A representative blot is shown from three independent experiments. Densitometry is shown for the replicate blots; ( C ) Cell culture supernatants were harvested, centrifuged to remove cellular debris and concentrated using <t>Amicon</t> <t>Ultra-4</t> Centrifugal Filter Devices (Millipore). Gelatin substrate zymography was used to separate and assess MMP-9 and MMP-2 activity. Clear areas represent areas of gelatinolytic activity. A representative zymogram is shown from three independent experiments. ** and *** indicates different levels of significant compared to time-matched control; * , ** and *** indicates varying levels of significance compared to treatment + inhibitor.
    Amicon Ultra 4 10k Centrifugal Filter, supplied by Merck & Co, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Merck & Co 10kda amicon ultra 4 centrifugal filters
    The specific blockade of TβRI kinase ablated Smad2 activation and dramatically affected the induction of the EMT markers, vimentin and MMPs by FK506. HK-2 cells were cultured on 6-well plates and treated with vehicle control, 5 µM FK506 with or without 500 nM of a TGFβRI/ALK5 kinase inhibitor V (SD-208). ( A ) Cells were incubated for 48 h with SD-208 concentrations ranging from 0–20μM. LDH and resazurin assays were conducted to assess SD-208 inhibitor toxicity; ( B ) Whole cell protein was extracted at 48 h with RIPA buffer. Equal amounts of protein were separated by SDS-PAGE electrophoresis, transferred to nitrocellulose and indirectly probed for vimentin and fibronectin using a mAb and ECL detection system. Whole Smad2 and GAPDH was used as a loading control. A representative blot is shown from three independent experiments. Densitometry is shown for the replicate blots; ( C ) Cell culture supernatants were harvested, centrifuged to remove cellular debris and concentrated using <t>Amicon</t> <t>Ultra-4</t> Centrifugal Filter Devices (Millipore). Gelatin substrate zymography was used to separate and assess MMP-9 and MMP-2 activity. Clear areas represent areas of gelatinolytic activity. A representative zymogram is shown from three independent experiments. ** and *** indicates different levels of significant compared to time-matched control; * , ** and *** indicates varying levels of significance compared to treatment + inhibitor.
    10kda Amicon Ultra 4 Centrifugal Filters, supplied by Merck & Co, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    The specific blockade of TβRI kinase ablated Smad2 activation and dramatically affected the induction of the EMT markers, vimentin and MMPs by FK506. HK-2 cells were cultured on 6-well plates and treated with vehicle control, 5 µM FK506 with or without 500 nM of a TGFβRI/ALK5 kinase inhibitor V (SD-208). ( A ) Cells were incubated for 48 h with SD-208 concentrations ranging from 0–20μM. LDH and resazurin assays were conducted to assess SD-208 inhibitor toxicity; ( B ) Whole cell protein was extracted at 48 h with RIPA buffer. Equal amounts of protein were separated by SDS-PAGE electrophoresis, transferred to nitrocellulose and indirectly probed for vimentin and fibronectin using a mAb and ECL detection system. Whole Smad2 and GAPDH was used as a loading control. A representative blot is shown from three independent experiments. Densitometry is shown for the replicate blots; ( C ) Cell culture supernatants were harvested, centrifuged to remove cellular debris and concentrated using Amicon Ultra-4 Centrifugal Filter Devices (Millipore). Gelatin substrate zymography was used to separate and assess MMP-9 and MMP-2 activity. Clear areas represent areas of gelatinolytic activity. A representative zymogram is shown from three independent experiments. ** and *** indicates different levels of significant compared to time-matched control; * , ** and *** indicates varying levels of significance compared to treatment + inhibitor.

    Journal: Journal of Clinical Medicine

    Article Title: Tacrolimus Modulates TGF-β Signaling to Induce Epithelial-Mesenchymal Transition in Human Renal Proximal Tubule Epithelial Cells

    doi: 10.3390/jcm5050050

    Figure Lengend Snippet: The specific blockade of TβRI kinase ablated Smad2 activation and dramatically affected the induction of the EMT markers, vimentin and MMPs by FK506. HK-2 cells were cultured on 6-well plates and treated with vehicle control, 5 µM FK506 with or without 500 nM of a TGFβRI/ALK5 kinase inhibitor V (SD-208). ( A ) Cells were incubated for 48 h with SD-208 concentrations ranging from 0–20μM. LDH and resazurin assays were conducted to assess SD-208 inhibitor toxicity; ( B ) Whole cell protein was extracted at 48 h with RIPA buffer. Equal amounts of protein were separated by SDS-PAGE electrophoresis, transferred to nitrocellulose and indirectly probed for vimentin and fibronectin using a mAb and ECL detection system. Whole Smad2 and GAPDH was used as a loading control. A representative blot is shown from three independent experiments. Densitometry is shown for the replicate blots; ( C ) Cell culture supernatants were harvested, centrifuged to remove cellular debris and concentrated using Amicon Ultra-4 Centrifugal Filter Devices (Millipore). Gelatin substrate zymography was used to separate and assess MMP-9 and MMP-2 activity. Clear areas represent areas of gelatinolytic activity. A representative zymogram is shown from three independent experiments. ** and *** indicates different levels of significant compared to time-matched control; * , ** and *** indicates varying levels of significance compared to treatment + inhibitor.

    Article Snippet: Gelatin Zymography: Cell supernatants were concentrated using Amicon Ultra-4 Centrifugal Filter Devices (Millipore, Billerica, MA, USA).

    Techniques: Activation Assay, Cell Culture, Incubation, SDS Page, Electrophoresis, Zymography, Activity Assay