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  • 96
    Millipore amicon ultra 2
    Separation of fractions from the conditioned media of PDLSCs using centrifugal filter device and their effects on the M1 polarization of THP-1 cells. The conditioned media from PDLSCs or LPS-preconditioned PDLSCs were separated using the centrifugal filter device, <t>Amicon</t> ultra-2 100 kDa (Millipore), into high-molecular weight (HMW) proteins and low-molecular weight (LMW) proteins. They were applied to M0 THP-1 cell with IFN-γ and the mRNAs expression for M1 polarization were analyzed. ( A ) Schematic summary of the experimental process. ( B , C ) mRNAs expression of THP-1 cells treated with isolated HMW or LMW proteins. Relative mRNA expression of IL-6 (B) and TNF-α were analyzed by RT-qPCR. Data shown as the mean ± SD, n = 3, ns: Not significant, ** p
    Amicon Ultra 2, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 303 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amicon ultra 2/product/Millipore
    Average 96 stars, based on 303 article reviews
    Price from $9.99 to $1999.99
    amicon ultra 2 - by Bioz Stars, 2020-05
    96/100 stars
      Buy from Supplier

    91
    Merck KGaA amicon ultra 2
    Separation of fractions from the conditioned media of PDLSCs using centrifugal filter device and their effects on the M1 polarization of THP-1 cells. The conditioned media from PDLSCs or LPS-preconditioned PDLSCs were separated using the centrifugal filter device, <t>Amicon</t> ultra-2 100 kDa (Millipore), into high-molecular weight (HMW) proteins and low-molecular weight (LMW) proteins. They were applied to M0 THP-1 cell with IFN-γ and the mRNAs expression for M1 polarization were analyzed. ( A ) Schematic summary of the experimental process. ( B , C ) mRNAs expression of THP-1 cells treated with isolated HMW or LMW proteins. Relative mRNA expression of IL-6 (B) and TNF-α were analyzed by RT-qPCR. Data shown as the mean ± SD, n = 3, ns: Not significant, ** p
    Amicon Ultra 2, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 91/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amicon ultra 2/product/Merck KGaA
    Average 91 stars, based on 55 article reviews
    Price from $9.99 to $1999.99
    amicon ultra 2 - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    91
    Merck KGaA amicon ultra 2 10k
    Separation of fractions from the conditioned media of PDLSCs using centrifugal filter device and their effects on the M1 polarization of THP-1 cells. The conditioned media from PDLSCs or LPS-preconditioned PDLSCs were separated using the centrifugal filter device, <t>Amicon</t> ultra-2 100 kDa (Millipore), into high-molecular weight (HMW) proteins and low-molecular weight (LMW) proteins. They were applied to M0 THP-1 cell with IFN-γ and the mRNAs expression for M1 polarization were analyzed. ( A ) Schematic summary of the experimental process. ( B , C ) mRNAs expression of THP-1 cells treated with isolated HMW or LMW proteins. Relative mRNA expression of IL-6 (B) and TNF-α were analyzed by RT-qPCR. Data shown as the mean ± SD, n = 3, ns: Not significant, ** p
    Amicon Ultra 2 10k, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amicon ultra 2 10k/product/Merck KGaA
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    amicon ultra 2 10k - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    Image Search Results


    Separation of fractions from the conditioned media of PDLSCs using centrifugal filter device and their effects on the M1 polarization of THP-1 cells. The conditioned media from PDLSCs or LPS-preconditioned PDLSCs were separated using the centrifugal filter device, Amicon ultra-2 100 kDa (Millipore), into high-molecular weight (HMW) proteins and low-molecular weight (LMW) proteins. They were applied to M0 THP-1 cell with IFN-γ and the mRNAs expression for M1 polarization were analyzed. ( A ) Schematic summary of the experimental process. ( B , C ) mRNAs expression of THP-1 cells treated with isolated HMW or LMW proteins. Relative mRNA expression of IL-6 (B) and TNF-α were analyzed by RT-qPCR. Data shown as the mean ± SD, n = 3, ns: Not significant, ** p

    Journal: International Journal of Molecular Sciences

    Article Title: Lipopolysaccharide-Preconditioned Periodontal Ligament Stem Cells Induce M1 Polarization of Macrophages through Extracellular Vesicles

    doi: 10.3390/ijms19123843

    Figure Lengend Snippet: Separation of fractions from the conditioned media of PDLSCs using centrifugal filter device and their effects on the M1 polarization of THP-1 cells. The conditioned media from PDLSCs or LPS-preconditioned PDLSCs were separated using the centrifugal filter device, Amicon ultra-2 100 kDa (Millipore), into high-molecular weight (HMW) proteins and low-molecular weight (LMW) proteins. They were applied to M0 THP-1 cell with IFN-γ and the mRNAs expression for M1 polarization were analyzed. ( A ) Schematic summary of the experimental process. ( B , C ) mRNAs expression of THP-1 cells treated with isolated HMW or LMW proteins. Relative mRNA expression of IL-6 (B) and TNF-α were analyzed by RT-qPCR. Data shown as the mean ± SD, n = 3, ns: Not significant, ** p

    Article Snippet: To isolate EVs using the centrifugal filter device, Amicon ultra-2 100 kDa (Millipore) was used according to the manufacturer’s instruction.

    Techniques: Molecular Weight, Expressing, Isolation, Quantitative RT-PCR