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    Qiagen qiagen allprep kits
    Aberrant transcriptome expression pivotal to eBL lymphomagenesis. a Schematic illustration of the aberrant gene expression and <t>miRNA</t> mediated regulatory changes that would initiate lymphomagenesis as a result of <t>DNA</t> damage. Combined loss of p53 function due to small interfering <t>RNA-mediated</t> regulation of ATM and NLK together with upregulation of TFAP4, would facilitate survival of cells with the c-myc-Igh chromosomal translocation and MYC induced cell cycle progression initiating eBL tumor development. ATM checkpoint kinase, transduces genomic stress signals to halt cell cycle progression in response to DNA damage. It is critical in the regulation of apoptosis and lymphomagenesis in c-myc induced lymphomas. ATM is downregulated in eBL and it is targeted by 4 miRs that are Upregulated in eBL. NLK is required for the upregulation of P53 expression in response to DNA damage. It interacts with P53 to enhance its stability and activity by abrogating MDM2 mediated degradation. NLK is downregulated in eBL tumor cells and also targeted by 2 miRs that are upregulated in eBL tumor cells. TFAP4/AP4 is a central mediator of cell cycle progression in response to c-MYC activation. b RNA seq. Expression counts of MYC , TFAP4 , ATM and NLK in eBL tumor cells and GC B cells. c Hierarchical clustering of eBL and GC B cells based on the expression profiles of MYC , TFAP4 , ATM and NLK also revealed a clear separation of the two groups. d . miRNA seq. Expression counts of hsa-miR-26a-5p, hsa-miR-27b-3p, hsa-miR-30b-5p, miR-17~92-cluster members (hsa-miR-19b-3p, and hsa-miR-92a-3p), and let-7-family miRs (hsa-let-7a-5p, hsa-let-7b-5p, hsa-let-7d-5p, hsa-let-7e-5p, and hsa-let-7 g-5p) in eBL tumor cells and GC B cells
    Qiagen Allprep Kits, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiagen allprep kits/product/Qiagen
    Average 99 stars, based on 129 article reviews
    Price from $9.99 to $1999.99
    qiagen allprep kits - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Qiagen allprep dna rna micro qiagen kit
    <t>DNA</t> isolation. Comparison of two parallel <t>DNA/RNA</t> kits and one DNA isolation kit. Mean values were obtained from duplicates of aliquots of 1, 10, 20, 50, 100, 150 and 200×10 3 cells sorted by fluorescence activated cell sorting using forward scatter and side scatter.
    Allprep Dna Rna Micro Qiagen Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 152 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/allprep dna rna micro qiagen kit/product/Qiagen
    Average 99 stars, based on 152 article reviews
    Price from $9.99 to $1999.99
    allprep dna rna micro qiagen kit - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    84
    Qiagen allprep dna rna mirna universal kit qiagen
    <t>DNA</t> isolation. Comparison of two parallel <t>DNA/RNA</t> kits and one DNA isolation kit. Mean values were obtained from duplicates of aliquots of 1, 10, 20, 50, 100, 150 and 200×10 3 cells sorted by fluorescence activated cell sorting using forward scatter and side scatter.
    Allprep Dna Rna Mirna Universal Kit Qiagen, supplied by Qiagen, used in various techniques. Bioz Stars score: 84/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/allprep dna rna mirna universal kit qiagen/product/Qiagen
    Average 84 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    allprep dna rna mirna universal kit qiagen - by Bioz Stars, 2020-08
    84/100 stars
      Buy from Supplier

    Image Search Results


    Aberrant transcriptome expression pivotal to eBL lymphomagenesis. a Schematic illustration of the aberrant gene expression and miRNA mediated regulatory changes that would initiate lymphomagenesis as a result of DNA damage. Combined loss of p53 function due to small interfering RNA-mediated regulation of ATM and NLK together with upregulation of TFAP4, would facilitate survival of cells with the c-myc-Igh chromosomal translocation and MYC induced cell cycle progression initiating eBL tumor development. ATM checkpoint kinase, transduces genomic stress signals to halt cell cycle progression in response to DNA damage. It is critical in the regulation of apoptosis and lymphomagenesis in c-myc induced lymphomas. ATM is downregulated in eBL and it is targeted by 4 miRs that are Upregulated in eBL. NLK is required for the upregulation of P53 expression in response to DNA damage. It interacts with P53 to enhance its stability and activity by abrogating MDM2 mediated degradation. NLK is downregulated in eBL tumor cells and also targeted by 2 miRs that are upregulated in eBL tumor cells. TFAP4/AP4 is a central mediator of cell cycle progression in response to c-MYC activation. b RNA seq. Expression counts of MYC , TFAP4 , ATM and NLK in eBL tumor cells and GC B cells. c Hierarchical clustering of eBL and GC B cells based on the expression profiles of MYC , TFAP4 , ATM and NLK also revealed a clear separation of the two groups. d . miRNA seq. Expression counts of hsa-miR-26a-5p, hsa-miR-27b-3p, hsa-miR-30b-5p, miR-17~92-cluster members (hsa-miR-19b-3p, and hsa-miR-92a-3p), and let-7-family miRs (hsa-let-7a-5p, hsa-let-7b-5p, hsa-let-7d-5p, hsa-let-7e-5p, and hsa-let-7 g-5p) in eBL tumor cells and GC B cells

    Journal: BMC Cancer

    Article Title: Integrative microRNA and mRNA deep-sequencing expression profiling in endemic Burkitt lymphoma

    doi: 10.1186/s12885-017-3711-9

    Figure Lengend Snippet: Aberrant transcriptome expression pivotal to eBL lymphomagenesis. a Schematic illustration of the aberrant gene expression and miRNA mediated regulatory changes that would initiate lymphomagenesis as a result of DNA damage. Combined loss of p53 function due to small interfering RNA-mediated regulation of ATM and NLK together with upregulation of TFAP4, would facilitate survival of cells with the c-myc-Igh chromosomal translocation and MYC induced cell cycle progression initiating eBL tumor development. ATM checkpoint kinase, transduces genomic stress signals to halt cell cycle progression in response to DNA damage. It is critical in the regulation of apoptosis and lymphomagenesis in c-myc induced lymphomas. ATM is downregulated in eBL and it is targeted by 4 miRs that are Upregulated in eBL. NLK is required for the upregulation of P53 expression in response to DNA damage. It interacts with P53 to enhance its stability and activity by abrogating MDM2 mediated degradation. NLK is downregulated in eBL tumor cells and also targeted by 2 miRs that are upregulated in eBL tumor cells. TFAP4/AP4 is a central mediator of cell cycle progression in response to c-MYC activation. b RNA seq. Expression counts of MYC , TFAP4 , ATM and NLK in eBL tumor cells and GC B cells. c Hierarchical clustering of eBL and GC B cells based on the expression profiles of MYC , TFAP4 , ATM and NLK also revealed a clear separation of the two groups. d . miRNA seq. Expression counts of hsa-miR-26a-5p, hsa-miR-27b-3p, hsa-miR-30b-5p, miR-17~92-cluster members (hsa-miR-19b-3p, and hsa-miR-92a-3p), and let-7-family miRs (hsa-let-7a-5p, hsa-let-7b-5p, hsa-let-7d-5p, hsa-let-7e-5p, and hsa-let-7 g-5p) in eBL tumor cells and GC B cells

    Article Snippet: RNA and small RNA isolation Total RNA and Small RNA molecules were extracted from eBL FNA samples in RNAlater using the AllPrep DNA/RNA/miRNA Universal kit (Qiagen) according to manufacturer’s instructions.

    Techniques: Expressing, Small Interfering RNA, Translocation Assay, Activity Assay, Activation Assay, RNA Sequencing Assay

    Study workflow. RNA and DNA were simultaneously extracted from 23 breast cancer ER+/HER2− tumors and their paired adjacent non-malignant tissues. Strand-specific paired-end RNA sequencing and comparative genomic hybridization (CGH) were performed. Quality control steps and RNA-Seq validation were performed and led to the elimination of one patient due to poor strand specificity in this sample. This strategy allowed the study of the differential expression of ncNATs and PCTs between tumors and non-malignant tissues and performance of differential correlation analysis of ncNAT/PCT pairs. Three lists of genes with deregulated ncNAT expression in tumors that could potentially affect the corresponding PC expression were extracted, and their coding genes were subjected to survival analysis with an external cohort (TCGA).

    Journal: Scientific Reports

    Article Title: Transcriptome-wide analysis of natural antisense transcripts shows their potential role in breast cancer

    doi: 10.1038/s41598-017-17811-2

    Figure Lengend Snippet: Study workflow. RNA and DNA were simultaneously extracted from 23 breast cancer ER+/HER2− tumors and their paired adjacent non-malignant tissues. Strand-specific paired-end RNA sequencing and comparative genomic hybridization (CGH) were performed. Quality control steps and RNA-Seq validation were performed and led to the elimination of one patient due to poor strand specificity in this sample. This strategy allowed the study of the differential expression of ncNATs and PCTs between tumors and non-malignant tissues and performance of differential correlation analysis of ncNAT/PCT pairs. Three lists of genes with deregulated ncNAT expression in tumors that could potentially affect the corresponding PC expression were extracted, and their coding genes were subjected to survival analysis with an external cohort (TCGA).

    Article Snippet: DNA/RNA/miRNA extraction DNA, RNA and miRNA were simultaneously extracted using an All Prep DNA/RNA/miRNA Universal kit (Qiagen, Belgium) according to the manufacturer’s protocol.

    Techniques: RNA Sequencing Assay, Hybridization, Expressing

    Comparison of DNA/RNA yields and RNA quality (inset). (A) Comparison between the AllPrep standard protocol (APS; described below figure), enzymatic lysis (Enz), enzymatic/bead beating (Bead), and Mirvana extraction protocols (Mirvana). (B) Comparison between different iterations of the AP protocol, examining the effect of preservation method {P}, lysis conditions {L}, and other extraction modification steps {E}. The data presents changes in yield relative to a control, where each column shows the effect of one factor relative to a control that differed in that factor only. The control was extracted using either the AP standard protocol or a modification as indicated by the X-axis labels below the horizontal lines. The factor that was changed is indicated just below the data column. The dotted line indicates no change relative to control. All data represent averages of three extractions, with error bars indicating the 95% confidence interval, except for the effect of lysozyme, which averaged the relative yield effects from multiple samples (from DL, LH, and HR samples, three replicate extractions each). Inset: RIN = RNA integrity number quality (0 (poor)- 10 (high) range) assessment by Bioanalyzer; NT = no preservation treatment.

    Journal: PLoS ONE

    Article Title: RNA Preservation Agents and Nucleic Acid Extraction Method Bias Perceived Bacterial Community Composition

    doi: 10.1371/journal.pone.0121659

    Figure Lengend Snippet: Comparison of DNA/RNA yields and RNA quality (inset). (A) Comparison between the AllPrep standard protocol (APS; described below figure), enzymatic lysis (Enz), enzymatic/bead beating (Bead), and Mirvana extraction protocols (Mirvana). (B) Comparison between different iterations of the AP protocol, examining the effect of preservation method {P}, lysis conditions {L}, and other extraction modification steps {E}. The data presents changes in yield relative to a control, where each column shows the effect of one factor relative to a control that differed in that factor only. The control was extracted using either the AP standard protocol or a modification as indicated by the X-axis labels below the horizontal lines. The factor that was changed is indicated just below the data column. The dotted line indicates no change relative to control. All data represent averages of three extractions, with error bars indicating the 95% confidence interval, except for the effect of lysozyme, which averaged the relative yield effects from multiple samples (from DL, LH, and HR samples, three replicate extractions each). Inset: RIN = RNA integrity number quality (0 (poor)- 10 (high) range) assessment by Bioanalyzer; NT = no preservation treatment.

    Article Snippet: The combined extraction method was based on the AllPrep DNA/RNA/miRNA Universal kit protocol (Qiagen).

    Techniques: Lysis, Preserving, Modification

    DNA isolation. Comparison of two parallel DNA/RNA kits and one DNA isolation kit. Mean values were obtained from duplicates of aliquots of 1, 10, 20, 50, 100, 150 and 200×10 3 cells sorted by fluorescence activated cell sorting using forward scatter and side scatter.

    Journal: Journal of Clinical Pathology

    Article Title: Biobanking strategy and sample preprocessing for integrative research in monoclonal gammopathies

    doi: 10.1136/jclinpath-2017-204329

    Figure Lengend Snippet: DNA isolation. Comparison of two parallel DNA/RNA kits and one DNA isolation kit. Mean values were obtained from duplicates of aliquots of 1, 10, 20, 50, 100, 150 and 200×10 3 cells sorted by fluorescence activated cell sorting using forward scatter and side scatter.

    Article Snippet: Thus even though total yields of RNA provided by AllPrep DNA/RNA Micro Kit and RNeasy Micro Kit (Qiagen) were more or less comparable, the AllPrep DNA/RNA Micro Kit had an advantage of parallel isolation of both DNA and RNA from one sample and could better exploit a sample with a low number of cells.

    Techniques: DNA Extraction, Fluorescence, FACS

    RNA isolation from the multiple myeloma cell line RPMI 8226 using the AllPrep DNA/RNA Micro Kit. Whiskers show SD of the mean values from samples performed in triplicate.

    Journal: Journal of Clinical Pathology

    Article Title: Biobanking strategy and sample preprocessing for integrative research in monoclonal gammopathies

    doi: 10.1136/jclinpath-2017-204329

    Figure Lengend Snippet: RNA isolation from the multiple myeloma cell line RPMI 8226 using the AllPrep DNA/RNA Micro Kit. Whiskers show SD of the mean values from samples performed in triplicate.

    Article Snippet: Thus even though total yields of RNA provided by AllPrep DNA/RNA Micro Kit and RNeasy Micro Kit (Qiagen) were more or less comparable, the AllPrep DNA/RNA Micro Kit had an advantage of parallel isolation of both DNA and RNA from one sample and could better exploit a sample with a low number of cells.

    Techniques: Isolation

    Diagram of preprocessing of samples with small amounts of input material. For a typical number of aberrant cells in various monoclonal gammopathies (MGs), we suggest nucleic acid isolation strategies and expected yield and suitability for downstream applications. For the isolation of nucleic acids with ≤10 5 cells, the most efficient isolation kit was the AllPrep DNA/RNA Micro Kit. For less starting material or for improved DNA yield for next generation sequencing (NGS), amplification by REPLI-g Single Cell of REPLI-g Mini Kit is recommended. aDNA, amplified genomic DNA; ALA, light chain amyloidosis; CGH, comparative genomic hybridisation; MGUS, monoclonal gammopathy of undetermined significance; MM, multiple myeloma; MRD, minimal residual disease; PCL, plasma cell leukaemia.

    Journal: Journal of Clinical Pathology

    Article Title: Biobanking strategy and sample preprocessing for integrative research in monoclonal gammopathies

    doi: 10.1136/jclinpath-2017-204329

    Figure Lengend Snippet: Diagram of preprocessing of samples with small amounts of input material. For a typical number of aberrant cells in various monoclonal gammopathies (MGs), we suggest nucleic acid isolation strategies and expected yield and suitability for downstream applications. For the isolation of nucleic acids with ≤10 5 cells, the most efficient isolation kit was the AllPrep DNA/RNA Micro Kit. For less starting material or for improved DNA yield for next generation sequencing (NGS), amplification by REPLI-g Single Cell of REPLI-g Mini Kit is recommended. aDNA, amplified genomic DNA; ALA, light chain amyloidosis; CGH, comparative genomic hybridisation; MGUS, monoclonal gammopathy of undetermined significance; MM, multiple myeloma; MRD, minimal residual disease; PCL, plasma cell leukaemia.

    Article Snippet: Thus even though total yields of RNA provided by AllPrep DNA/RNA Micro Kit and RNeasy Micro Kit (Qiagen) were more or less comparable, the AllPrep DNA/RNA Micro Kit had an advantage of parallel isolation of both DNA and RNA from one sample and could better exploit a sample with a low number of cells.

    Techniques: Isolation, Next-Generation Sequencing, Amplification, Hybridization