allele-specific pcr Search Results


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    Roche allele specific polymerase chain reaction pcr analysis
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    Thermo Fisher custom designed competitive allele specific taqman cast polymerase chain reaction polymerase chain reaction pcr assay
    RPL39 (A14V) mutation status analyzed in metaplastic breast cancer. A) The relationship between ribosomal protein L39 (RPL39) expression and overall survival was assessed among 457 triple-negative breast cancer (TNBC) patients. The P value was calculated using a log-rank test. B) RPL39 A14V mutation rate was determined by competitive allele-specific <t>TaqMan</t> <t>(CAST)</t> polymerase chain reaction <t>(PCR)</t> analysis of tumor samples from patients with nonmetaplastic and metaplastic breast cancer (each n = 40). Fisher’s exact test was used to compare the mutation rate in metaplastic breast cancer vs nonmetaplastic breast cancer. PIK3CA mutations (H1047R and E545K) rates are also shown. C) The results of the CAST PCR analysis were confirmed by droplet digital PCR. Wilcoxon rank-sum test was used to determine statistically significant differences in the fractional abundance of the RPL39 A14V mutation between metaplastic breast cancer and TNBC. We used a threshold of 0.1% fractional abundance to define low RPL39 expression level. All statistical tests were two-sided. CI = confidence interval; HR = hazard ratio; MBC = metaplastic breast cancer; TNBC = triple-negative breast cancer.
    Custom Designed Competitive Allele Specific Taqman Cast Polymerase Chain Reaction Polymerase Chain Reaction Pcr Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    RPL39 (A14V) mutation status analyzed in metaplastic breast cancer. A) The relationship between ribosomal protein L39 (RPL39) expression and overall survival was assessed among 457 triple-negative breast cancer (TNBC) patients. The P value was calculated using a log-rank test. B) RPL39 A14V mutation rate was determined by competitive allele-specific <t>TaqMan</t> <t>(CAST)</t> polymerase chain reaction <t>(PCR)</t> analysis of tumor samples from patients with nonmetaplastic and metaplastic breast cancer (each n = 40). Fisher’s exact test was used to compare the mutation rate in metaplastic breast cancer vs nonmetaplastic breast cancer. PIK3CA mutations (H1047R and E545K) rates are also shown. C) The results of the CAST PCR analysis were confirmed by droplet digital PCR. Wilcoxon rank-sum test was used to determine statistically significant differences in the fractional abundance of the RPL39 A14V mutation between metaplastic breast cancer and TNBC. We used a threshold of 0.1% fractional abundance to define low RPL39 expression level. All statistical tests were two-sided. CI = confidence interval; HR = hazard ratio; MBC = metaplastic breast cancer; TNBC = triple-negative breast cancer.
    Allele Specific Primer Polymerase Chain Reaction Asp Pcr Method, supplied by Toyobo, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    EntroGen allele specific real time polymerase chain reaction based assay
    RPL39 (A14V) mutation status analyzed in metaplastic breast cancer. A) The relationship between ribosomal protein L39 (RPL39) expression and overall survival was assessed among 457 triple-negative breast cancer (TNBC) patients. The P value was calculated using a log-rank test. B) RPL39 A14V mutation rate was determined by competitive allele-specific <t>TaqMan</t> <t>(CAST)</t> polymerase chain reaction <t>(PCR)</t> analysis of tumor samples from patients with nonmetaplastic and metaplastic breast cancer (each n = 40). Fisher’s exact test was used to compare the mutation rate in metaplastic breast cancer vs nonmetaplastic breast cancer. PIK3CA mutations (H1047R and E545K) rates are also shown. C) The results of the CAST PCR analysis were confirmed by droplet digital PCR. Wilcoxon rank-sum test was used to determine statistically significant differences in the fractional abundance of the RPL39 A14V mutation between metaplastic breast cancer and TNBC. We used a threshold of 0.1% fractional abundance to define low RPL39 expression level. All statistical tests were two-sided. CI = confidence interval; HR = hazard ratio; MBC = metaplastic breast cancer; TNBC = triple-negative breast cancer.
    Allele Specific Real Time Polymerase Chain Reaction Based Assay, supplied by EntroGen, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    RPL39 (A14V) mutation status analyzed in metaplastic breast cancer. A) The relationship between ribosomal protein L39 (RPL39) expression and overall survival was assessed among 457 triple-negative breast cancer (TNBC) patients. The P value was calculated using a log-rank test. B) RPL39 A14V mutation rate was determined by competitive allele-specific TaqMan (CAST) polymerase chain reaction (PCR) analysis of tumor samples from patients with nonmetaplastic and metaplastic breast cancer (each n = 40). Fisher’s exact test was used to compare the mutation rate in metaplastic breast cancer vs nonmetaplastic breast cancer. PIK3CA mutations (H1047R and E545K) rates are also shown. C) The results of the CAST PCR analysis were confirmed by droplet digital PCR. Wilcoxon rank-sum test was used to determine statistically significant differences in the fractional abundance of the RPL39 A14V mutation between metaplastic breast cancer and TNBC. We used a threshold of 0.1% fractional abundance to define low RPL39 expression level. All statistical tests were two-sided. CI = confidence interval; HR = hazard ratio; MBC = metaplastic breast cancer; TNBC = triple-negative breast cancer.

    Journal: JNCI Journal of the National Cancer Institute

    Article Title: Role of RPL39 in Metaplastic Breast Cancer

    doi: 10.1093/jnci/djw292

    Figure Lengend Snippet: RPL39 (A14V) mutation status analyzed in metaplastic breast cancer. A) The relationship between ribosomal protein L39 (RPL39) expression and overall survival was assessed among 457 triple-negative breast cancer (TNBC) patients. The P value was calculated using a log-rank test. B) RPL39 A14V mutation rate was determined by competitive allele-specific TaqMan (CAST) polymerase chain reaction (PCR) analysis of tumor samples from patients with nonmetaplastic and metaplastic breast cancer (each n = 40). Fisher’s exact test was used to compare the mutation rate in metaplastic breast cancer vs nonmetaplastic breast cancer. PIK3CA mutations (H1047R and E545K) rates are also shown. C) The results of the CAST PCR analysis were confirmed by droplet digital PCR. Wilcoxon rank-sum test was used to determine statistically significant differences in the fractional abundance of the RPL39 A14V mutation between metaplastic breast cancer and TNBC. We used a threshold of 0.1% fractional abundance to define low RPL39 expression level. All statistical tests were two-sided. CI = confidence interval; HR = hazard ratio; MBC = metaplastic breast cancer; TNBC = triple-negative breast cancer.

    Article Snippet: Genomic DNA isolated from patient tissue samples was analyzed for the presence of wild-type or mutant allele using a custom-designed competitive allele-specific TaqMan (CAST) polymerase chain reaction (PCR) assay (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s instructions.

    Techniques: Mutagenesis, Expressing, Polymerase Chain Reaction, Digital PCR