alkaline phosphatase-conjugated streptavidin Search Results


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    Alkaline Phosphatase Conjugated Streptavidin
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    94
    Vector Laboratories streptavidin alkaline phosphatase
    Streptavidin Alkaline Phosphatase, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 334 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin alkaline phosphatase/product/Vector Laboratories
    Average 94 stars, based on 334 article reviews
    Price from $9.99 to $1999.99
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    95
    Thermo Fisher alkaline phosphatase conjugated streptavidin
    INCA preferentially interacts with caspase-1 CARD . ( a ) Co-expression of His-GFP-ASC CARD and biotinylated INCA showed no significant interaction between INCA and ASC CARD , as indicated by the Western blot. ( b ) Over-stoichiometric amounts of INCA did not inhibit His-GFP-ASC CARD nucleated ASC CARD filament formation. Averages of triplicate experiments were plotted. ( c ) Co-expression of His-GFP-caspase-1 CARD and biotinylated INCA showed interaction between INCA and caspase-1 CARD , as confirmed by the PAGE gel. ( d ) Sub-stoichiometric amounts of INCA inhibited His-GFP-caspase-1 CARD nucleated caspase-1 CARD filament formation. Averages of duplicate experiments were plotted. ( e–f ) Negative-stain electron micrographs of the complex (as shown in panel c at 7 ml) between His-GFP-caspase-1 CARD and biotinylated INCA after <t>streptavidin-gold</t> labeling ( e ) or Ni-NTA-gold labeling ( f ).
    Alkaline Phosphatase Conjugated Streptavidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 447 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Jackson Immuno alkaline phosphatase conjugated streptavidin
    INCA preferentially interacts with caspase-1 CARD . ( a ) Co-expression of His-GFP-ASC CARD and biotinylated INCA showed no significant interaction between INCA and ASC CARD , as indicated by the Western blot. ( b ) Over-stoichiometric amounts of INCA did not inhibit His-GFP-ASC CARD nucleated ASC CARD filament formation. Averages of triplicate experiments were plotted. ( c ) Co-expression of His-GFP-caspase-1 CARD and biotinylated INCA showed interaction between INCA and caspase-1 CARD , as confirmed by the PAGE gel. ( d ) Sub-stoichiometric amounts of INCA inhibited His-GFP-caspase-1 CARD nucleated caspase-1 CARD filament formation. Averages of duplicate experiments were plotted. ( e–f ) Negative-stain electron micrographs of the complex (as shown in panel c at 7 ml) between His-GFP-caspase-1 CARD and biotinylated INCA after <t>streptavidin-gold</t> labeling ( e ) or Ni-NTA-gold labeling ( f ).
    Alkaline Phosphatase Conjugated Streptavidin, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 329 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore alkaline phosphatase conjugated streptavidin
    INCA preferentially interacts with caspase-1 CARD . ( a ) Co-expression of His-GFP-ASC CARD and biotinylated INCA showed no significant interaction between INCA and ASC CARD , as indicated by the Western blot. ( b ) Over-stoichiometric amounts of INCA did not inhibit His-GFP-ASC CARD nucleated ASC CARD filament formation. Averages of triplicate experiments were plotted. ( c ) Co-expression of His-GFP-caspase-1 CARD and biotinylated INCA showed interaction between INCA and caspase-1 CARD , as confirmed by the PAGE gel. ( d ) Sub-stoichiometric amounts of INCA inhibited His-GFP-caspase-1 CARD nucleated caspase-1 CARD filament formation. Averages of duplicate experiments were plotted. ( e–f ) Negative-stain electron micrographs of the complex (as shown in panel c at 7 ml) between His-GFP-caspase-1 CARD and biotinylated INCA after <t>streptavidin-gold</t> labeling ( e ) or Ni-NTA-gold labeling ( f ).
    Alkaline Phosphatase Conjugated Streptavidin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 356 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies alkaline phosphatase conjugated streptavidin
    INCA preferentially interacts with caspase-1 CARD . ( a ) Co-expression of His-GFP-ASC CARD and biotinylated INCA showed no significant interaction between INCA and ASC CARD , as indicated by the Western blot. ( b ) Over-stoichiometric amounts of INCA did not inhibit His-GFP-ASC CARD nucleated ASC CARD filament formation. Averages of triplicate experiments were plotted. ( c ) Co-expression of His-GFP-caspase-1 CARD and biotinylated INCA showed interaction between INCA and caspase-1 CARD , as confirmed by the PAGE gel. ( d ) Sub-stoichiometric amounts of INCA inhibited His-GFP-caspase-1 CARD nucleated caspase-1 CARD filament formation. Averages of duplicate experiments were plotted. ( e–f ) Negative-stain electron micrographs of the complex (as shown in panel c at 7 ml) between His-GFP-caspase-1 CARD and biotinylated INCA after <t>streptavidin-gold</t> labeling ( e ) or Ni-NTA-gold labeling ( f ).
    Alkaline Phosphatase Conjugated Streptavidin, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 328 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mab Technologies streptavidin alkaline phosphatase conjugate
    INCA preferentially interacts with caspase-1 CARD . ( a ) Co-expression of His-GFP-ASC CARD and biotinylated INCA showed no significant interaction between INCA and ASC CARD , as indicated by the Western blot. ( b ) Over-stoichiometric amounts of INCA did not inhibit His-GFP-ASC CARD nucleated ASC CARD filament formation. Averages of triplicate experiments were plotted. ( c ) Co-expression of His-GFP-caspase-1 CARD and biotinylated INCA showed interaction between INCA and caspase-1 CARD , as confirmed by the PAGE gel. ( d ) Sub-stoichiometric amounts of INCA inhibited His-GFP-caspase-1 CARD nucleated caspase-1 CARD filament formation. Averages of duplicate experiments were plotted. ( e–f ) Negative-stain electron micrographs of the complex (as shown in panel c at 7 ml) between His-GFP-caspase-1 CARD and biotinylated INCA after <t>streptavidin-gold</t> labeling ( e ) or Ni-NTA-gold labeling ( f ).
    Streptavidin Alkaline Phosphatase Conjugate, supplied by Mab Technologies, used in various techniques. Bioz Stars score: 92/100, based on 171 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mab Technologies streptavidin conjugated alkaline phosphatase
    INCA preferentially interacts with caspase-1 CARD . ( a ) Co-expression of His-GFP-ASC CARD and biotinylated INCA showed no significant interaction between INCA and ASC CARD , as indicated by the Western blot. ( b ) Over-stoichiometric amounts of INCA did not inhibit His-GFP-ASC CARD nucleated ASC CARD filament formation. Averages of triplicate experiments were plotted. ( c ) Co-expression of His-GFP-caspase-1 CARD and biotinylated INCA showed interaction between INCA and caspase-1 CARD , as confirmed by the PAGE gel. ( d ) Sub-stoichiometric amounts of INCA inhibited His-GFP-caspase-1 CARD nucleated caspase-1 CARD filament formation. Averages of duplicate experiments were plotted. ( e–f ) Negative-stain electron micrographs of the complex (as shown in panel c at 7 ml) between His-GFP-caspase-1 CARD and biotinylated INCA after <t>streptavidin-gold</t> labeling ( e ) or Ni-NTA-gold labeling ( f ).
    Streptavidin Conjugated Alkaline Phosphatase, supplied by Mab Technologies, used in various techniques. Bioz Stars score: 92/100, based on 237 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Vector Laboratories alkaline phosphatase conjugated streptavidin
    INCA preferentially interacts with caspase-1 CARD . ( a ) Co-expression of His-GFP-ASC CARD and biotinylated INCA showed no significant interaction between INCA and ASC CARD , as indicated by the Western blot. ( b ) Over-stoichiometric amounts of INCA did not inhibit His-GFP-ASC CARD nucleated ASC CARD filament formation. Averages of triplicate experiments were plotted. ( c ) Co-expression of His-GFP-caspase-1 CARD and biotinylated INCA showed interaction between INCA and caspase-1 CARD , as confirmed by the PAGE gel. ( d ) Sub-stoichiometric amounts of INCA inhibited His-GFP-caspase-1 CARD nucleated caspase-1 CARD filament formation. Averages of duplicate experiments were plotted. ( e–f ) Negative-stain electron micrographs of the complex (as shown in panel c at 7 ml) between His-GFP-caspase-1 CARD and biotinylated INCA after <t>streptavidin-gold</t> labeling ( e ) or Ni-NTA-gold labeling ( f ).
    Alkaline Phosphatase Conjugated Streptavidin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 253 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Thermo Fisher streptavidin alkaline phosphatase conjugate
    Biotinylated DNA tether binds <t>streptavidin.</t> (A) Schematic of the double-stranded DNA tether prepared from half lambda DNA that contains functional groups as indicated on both ends of the molecule. (B) A representative force-extension curve for a single DNA tether formed between an anti-digoxigenin coated polystyrene particle and a biotin-coated polystyrene particle. The length of the overstretch transition in this figure measures 5.4 |im, consistent with that of a half-lambda DNA.
    Streptavidin Alkaline Phosphatase Conjugate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 266 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    GE Healthcare alkaline phosphatase conjugated streptavidin
    Biotinylated DNA tether binds <t>streptavidin.</t> (A) Schematic of the double-stranded DNA tether prepared from half lambda DNA that contains functional groups as indicated on both ends of the molecule. (B) A representative force-extension curve for a single DNA tether formed between an anti-digoxigenin coated polystyrene particle and a biotin-coated polystyrene particle. The length of the overstretch transition in this figure measures 5.4 |im, consistent with that of a half-lambda DNA.
    Alkaline Phosphatase Conjugated Streptavidin, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 96/100, based on 201 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    alkaline phosphatase conjugated streptavidin - by Bioz Stars, 2021-01
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    98
    Millipore streptavidin alkaline phosphatase conjugate
    Biotinylated DNA tether binds <t>streptavidin.</t> (A) Schematic of the double-stranded DNA tether prepared from half lambda DNA that contains functional groups as indicated on both ends of the molecule. (B) A representative force-extension curve for a single DNA tether formed between an anti-digoxigenin coated polystyrene particle and a biotin-coated polystyrene particle. The length of the overstretch transition in this figure measures 5.4 |im, consistent with that of a half-lambda DNA.
    Streptavidin Alkaline Phosphatase Conjugate, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 212 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Thermo Fisher streptavidin alkaline phosphatase
    Biotinylated DNA tether binds <t>streptavidin.</t> (A) Schematic of the double-stranded DNA tether prepared from half lambda DNA that contains functional groups as indicated on both ends of the molecule. (B) A representative force-extension curve for a single DNA tether formed between an anti-digoxigenin coated polystyrene particle and a biotin-coated polystyrene particle. The length of the overstretch transition in this figure measures 5.4 |im, consistent with that of a half-lambda DNA.
    Streptavidin Alkaline Phosphatase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 727 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    streptavidin alkaline phosphatase - by Bioz Stars, 2021-01
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    92
    Biogenex alkaline phosphatase conjugated streptavidin
    Biotinylated DNA tether binds <t>streptavidin.</t> (A) Schematic of the double-stranded DNA tether prepared from half lambda DNA that contains functional groups as indicated on both ends of the molecule. (B) A representative force-extension curve for a single DNA tether formed between an anti-digoxigenin coated polystyrene particle and a biotin-coated polystyrene particle. The length of the overstretch transition in this figure measures 5.4 |im, consistent with that of a half-lambda DNA.
    Alkaline Phosphatase Conjugated Streptavidin, supplied by Biogenex, used in various techniques. Bioz Stars score: 92/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Boehringer Mannheim streptavidin conjugated alkaline phosphatase
    Biotinylated DNA tether binds <t>streptavidin.</t> (A) Schematic of the double-stranded DNA tether prepared from half lambda DNA that contains functional groups as indicated on both ends of the molecule. (B) A representative force-extension curve for a single DNA tether formed between an anti-digoxigenin coated polystyrene particle and a biotin-coated polystyrene particle. The length of the overstretch transition in this figure measures 5.4 |im, consistent with that of a half-lambda DNA.
    Streptavidin Conjugated Alkaline Phosphatase, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Boehringer Mannheim streptavidin alkaline phosphatase conjugate
    Analysis of column fractions from total soybean seed extracts after chromatography through antibody-affinity columns. Lanes 1, Protein-gel blot probed with <t>streptavidin-alkaline</t> phosphatase conjugate; lanes 2, anti-BCCP probe; lanes 3, anti-BC probe; lanes 4, anti-α-CT probe; lanes 5, anti-β-CT probe. Gly eluates from each column (indicated above the lanes) were separated on 9% SDS-PAGE and transferred to PVDF membranes. Each membrane was subsequently cut into strips and probed with each ACCase antibody.
    Streptavidin Alkaline Phosphatase Conjugate, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Promega streptavidin conjugated alkaline phosphatase
    Analysis of column fractions from total soybean seed extracts after chromatography through antibody-affinity columns. Lanes 1, Protein-gel blot probed with <t>streptavidin-alkaline</t> phosphatase conjugate; lanes 2, anti-BCCP probe; lanes 3, anti-BC probe; lanes 4, anti-α-CT probe; lanes 5, anti-β-CT probe. Gly eluates from each column (indicated above the lanes) were separated on 9% SDS-PAGE and transferred to PVDF membranes. Each membrane was subsequently cut into strips and probed with each ACCase antibody.
    Streptavidin Conjugated Alkaline Phosphatase, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    streptavidin conjugated alkaline phosphatase - by Bioz Stars, 2021-01
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    N/A
    STREPTAVIDIN ALKALINE PHOSPHATASE Conjugated S000 05
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    N/A
    Streptavidin conjugated to alkaline phosphatase This product was developed for use with TSA systems
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    Image Search Results


    INCA preferentially interacts with caspase-1 CARD . ( a ) Co-expression of His-GFP-ASC CARD and biotinylated INCA showed no significant interaction between INCA and ASC CARD , as indicated by the Western blot. ( b ) Over-stoichiometric amounts of INCA did not inhibit His-GFP-ASC CARD nucleated ASC CARD filament formation. Averages of triplicate experiments were plotted. ( c ) Co-expression of His-GFP-caspase-1 CARD and biotinylated INCA showed interaction between INCA and caspase-1 CARD , as confirmed by the PAGE gel. ( d ) Sub-stoichiometric amounts of INCA inhibited His-GFP-caspase-1 CARD nucleated caspase-1 CARD filament formation. Averages of duplicate experiments were plotted. ( e–f ) Negative-stain electron micrographs of the complex (as shown in panel c at 7 ml) between His-GFP-caspase-1 CARD and biotinylated INCA after streptavidin-gold labeling ( e ) or Ni-NTA-gold labeling ( f ).

    Journal: Nature structural & molecular biology

    Article Title: Molecular basis of caspase-1 polymerization and its inhibition by a novel capping mechanism

    doi: 10.1038/nsmb.3199

    Figure Lengend Snippet: INCA preferentially interacts with caspase-1 CARD . ( a ) Co-expression of His-GFP-ASC CARD and biotinylated INCA showed no significant interaction between INCA and ASC CARD , as indicated by the Western blot. ( b ) Over-stoichiometric amounts of INCA did not inhibit His-GFP-ASC CARD nucleated ASC CARD filament formation. Averages of triplicate experiments were plotted. ( c ) Co-expression of His-GFP-caspase-1 CARD and biotinylated INCA showed interaction between INCA and caspase-1 CARD , as confirmed by the PAGE gel. ( d ) Sub-stoichiometric amounts of INCA inhibited His-GFP-caspase-1 CARD nucleated caspase-1 CARD filament formation. Averages of duplicate experiments were plotted. ( e–f ) Negative-stain electron micrographs of the complex (as shown in panel c at 7 ml) between His-GFP-caspase-1 CARD and biotinylated INCA after streptavidin-gold labeling ( e ) or Ni-NTA-gold labeling ( f ).

    Article Snippet: The presence of biotin was confirmed by Western blot using streptavidin-conjugated alkaline phosphatase (streptavidine-AP, Molecular Probes Cat# S921).

    Techniques: Expressing, Western Blot, Polyacrylamide Gel Electrophoresis, Staining, Labeling

    ICEBERG interacts with caspase-1 CARD by co-mixing. ( a ) Co-expression of His-GFP-caspase-1 CARD and biotinylated ICEBERG. Streptavidin-AP and anti-6×His-tag Western blots were shown to confirm the presence of ICEBERG in complex with His-GFP-caspase-1 CARD after Ni-NTA affinity chromatography. L: Whole cell lysate. S: Soluble fraction. U: Ni-NTA unbound. E: Ni-NTA elute. ( b ) The complex eluted from the void of a Superdex 200 column. ( c–d ) Negative-stain electron micrographs of the complex (as shown in panel b at 7 ml) between His-GFP-caspase-1 CARD and biotinylated ICEBERG after Ni-NTA-gold labeling ( c ) or streptavidin-gold labeling ( d ).

    Journal: Nature structural & molecular biology

    Article Title: Molecular basis of caspase-1 polymerization and its inhibition by a novel capping mechanism

    doi: 10.1038/nsmb.3199

    Figure Lengend Snippet: ICEBERG interacts with caspase-1 CARD by co-mixing. ( a ) Co-expression of His-GFP-caspase-1 CARD and biotinylated ICEBERG. Streptavidin-AP and anti-6×His-tag Western blots were shown to confirm the presence of ICEBERG in complex with His-GFP-caspase-1 CARD after Ni-NTA affinity chromatography. L: Whole cell lysate. S: Soluble fraction. U: Ni-NTA unbound. E: Ni-NTA elute. ( b ) The complex eluted from the void of a Superdex 200 column. ( c–d ) Negative-stain electron micrographs of the complex (as shown in panel b at 7 ml) between His-GFP-caspase-1 CARD and biotinylated ICEBERG after Ni-NTA-gold labeling ( c ) or streptavidin-gold labeling ( d ).

    Article Snippet: The presence of biotin was confirmed by Western blot using streptavidin-conjugated alkaline phosphatase (streptavidine-AP, Molecular Probes Cat# S921).

    Techniques: Expressing, Western Blot, Affinity Chromatography, Staining, Labeling

    Biotinylated DNA tether binds streptavidin. (A) Schematic of the double-stranded DNA tether prepared from half lambda DNA that contains functional groups as indicated on both ends of the molecule. (B) A representative force-extension curve for a single DNA tether formed between an anti-digoxigenin coated polystyrene particle and a biotin-coated polystyrene particle. The length of the overstretch transition in this figure measures 5.4 |im, consistent with that of a half-lambda DNA.

    Journal: Proceedings of SPIE--the International Society for Optical Engineering

    Article Title: A method for tethering single viral particles for virus-cell interaction studies with optical tweezers

    doi: 10.1117/12.2500050

    Figure Lengend Snippet: Biotinylated DNA tether binds streptavidin. (A) Schematic of the double-stranded DNA tether prepared from half lambda DNA that contains functional groups as indicated on both ends of the molecule. (B) A representative force-extension curve for a single DNA tether formed between an anti-digoxigenin coated polystyrene particle and a biotin-coated polystyrene particle. The length of the overstretch transition in this figure measures 5.4 |im, consistent with that of a half-lambda DNA.

    Article Snippet: Streptavidin-alkaline phosphatase conjugate (Invitrogen, Catalog #43–4322) at a concentration of 1.5 ug/mL was used to detect biotinylated protein.

    Techniques: Lambda DNA Preparation, Functional Assay

    Metabolic biotinylation of individual HIV-1 virions. (A) Western blotting of virion preparations with varied inputs of TfR (indicated in the figure) and BirA expression plasmid detected with streptavidin-alkaline phosphatase conjugate. Loaded samples were diluted to have equal p24 concentrations. Lysate from 293T cells transfected with pTfR-AP-IRES-BirA-ER (pTfR) serves as a positive control. (B) Optical trapping virometry results for individual HIV-1 virions: Left: histogram of Alexa 594 fluorescence, proportional to the number of biotinylated transferrin receptors, in individually trapped virions. Right: histogram of EGFP fluorescence in individually trapped virions. N = 28 virions trapped on the same day.

    Journal: Proceedings of SPIE--the International Society for Optical Engineering

    Article Title: A method for tethering single viral particles for virus-cell interaction studies with optical tweezers

    doi: 10.1117/12.2500050

    Figure Lengend Snippet: Metabolic biotinylation of individual HIV-1 virions. (A) Western blotting of virion preparations with varied inputs of TfR (indicated in the figure) and BirA expression plasmid detected with streptavidin-alkaline phosphatase conjugate. Loaded samples were diluted to have equal p24 concentrations. Lysate from 293T cells transfected with pTfR-AP-IRES-BirA-ER (pTfR) serves as a positive control. (B) Optical trapping virometry results for individual HIV-1 virions: Left: histogram of Alexa 594 fluorescence, proportional to the number of biotinylated transferrin receptors, in individually trapped virions. Right: histogram of EGFP fluorescence in individually trapped virions. N = 28 virions trapped on the same day.

    Article Snippet: Streptavidin-alkaline phosphatase conjugate (Invitrogen, Catalog #43–4322) at a concentration of 1.5 ug/mL was used to detect biotinylated protein.

    Techniques: Western Blot, Expressing, Plasmid Preparation, Transfection, Positive Control, Fluorescence

    Analysis of column fractions from total soybean seed extracts after chromatography through antibody-affinity columns. Lanes 1, Protein-gel blot probed with streptavidin-alkaline phosphatase conjugate; lanes 2, anti-BCCP probe; lanes 3, anti-BC probe; lanes 4, anti-α-CT probe; lanes 5, anti-β-CT probe. Gly eluates from each column (indicated above the lanes) were separated on 9% SDS-PAGE and transferred to PVDF membranes. Each membrane was subsequently cut into strips and probed with each ACCase antibody.

    Journal: Plant Physiology

    Article Title: A Multisubunit Acetyl Coenzyme A Carboxylase from Soybean 1

    doi:

    Figure Lengend Snippet: Analysis of column fractions from total soybean seed extracts after chromatography through antibody-affinity columns. Lanes 1, Protein-gel blot probed with streptavidin-alkaline phosphatase conjugate; lanes 2, anti-BCCP probe; lanes 3, anti-BC probe; lanes 4, anti-α-CT probe; lanes 5, anti-β-CT probe. Gly eluates from each column (indicated above the lanes) were separated on 9% SDS-PAGE and transferred to PVDF membranes. Each membrane was subsequently cut into strips and probed with each ACCase antibody.

    Article Snippet: Biotinylated proteins were detected with a streptavidin-alkaline phosphatase conjugate (Boehringer Mannheim) diluted 1:2,000.

    Techniques: Chromatography, Western Blot, SDS Page

    ) at 0.05 mL/min. Fractions (0.75 mL) were collected, and a high-molecular-mass gel-filtration calibration kit (Pharmacia) was used to calibrate the column. Eluted protein fractions were separated on SDS-PAGE, blotted to nylon membranes, and analyzed with different antibodies as indicated. strept, Streptavidin-alkaline phosphatase conjugate. The results from the protein-gel blot are aligned with the chromatographic profile. Approximate positions of electrophoretic molecular-mass markers are shown at the right of the protein-blot panels. The position of Blue Dextran 2000 (BD) and other markers used to calibrate the column are shown above the elution profile. The markers were thryoglobulin (667 kD), ferritin (440 kD), catalase (232 kD), and aldolase (158 kD).

    Journal: Plant Physiology

    Article Title: A Multisubunit Acetyl Coenzyme A Carboxylase from Soybean 1

    doi:

    Figure Lengend Snippet: ) at 0.05 mL/min. Fractions (0.75 mL) were collected, and a high-molecular-mass gel-filtration calibration kit (Pharmacia) was used to calibrate the column. Eluted protein fractions were separated on SDS-PAGE, blotted to nylon membranes, and analyzed with different antibodies as indicated. strept, Streptavidin-alkaline phosphatase conjugate. The results from the protein-gel blot are aligned with the chromatographic profile. Approximate positions of electrophoretic molecular-mass markers are shown at the right of the protein-blot panels. The position of Blue Dextran 2000 (BD) and other markers used to calibrate the column are shown above the elution profile. The markers were thryoglobulin (667 kD), ferritin (440 kD), catalase (232 kD), and aldolase (158 kD).

    Article Snippet: Biotinylated proteins were detected with a streptavidin-alkaline phosphatase conjugate (Boehringer Mannheim) diluted 1:2,000.

    Techniques: Filtration, SDS Page, Western Blot