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  • 99
    Thermo Fisher alexa488 conjugated goat anti mouse igg
    Alexa488 Conjugated Goat Anti Mouse Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 348 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher alexa488 conjugated goat anti rat igg
    Representative images of PET, radioluminography, in vivo fluorescence imaging, and ex vivo (fluorescence) microscopy of A375-pIRES/-EphB4 tumor-bearing NMRI nu/nu mice and tumor cryosections. After intravenous injection [ 18 F]FDG, [ 64 Cu]Cu-ETS, and [ 18 F]FMISO clearly accumulate in A375-pIRES and A375-EphB4 tumors and can be visualized by small animal PET ( A – C ) and subsequent radioluminography ( D – F ). ( A – C ) PET data are illustrated as maximum intensity projection (MIPs) of tracer-dependent frame times ([ 18 F]FDG, 30–60 min p.i.; [ 64 Cu]Cu-ETS, 0–30 min p.i.; [ 18 F]FMISO, 180–240 min p.i.). ( D – F ) Subsequent to PET imaging, A375-pIRES/-EphB4 tumors were resected and distribution of PET tracers was analyzed by radioluminography in tumor cryosections (18 sections each in 3 tumor depth). ( G ) In vivo fluorescence imaging of A375-pIRES/-EphB4 tumor-bearing NMRI nu/nu mice 24 h post i.v. injection of AngioSense (750/790 nm). ( H ) Ex vivo fluorescence microscopy of A375-pIRES/-EphB4 tumor cryosections following i.v. injection of 30 mg/kg Hoechst 33342 exactly 1 min before sacrifice of mice. ( I ) Tumor cryosections were stained with CD31 and LYVE-1 specific primary antibodies as well as <t>Alexa</t> Fluor 488- and Alexa 546-conjugated secondary antibodies to determine blood and lymph vessel markers CD31 (colored in green) and LYVE-1 (colored in orange), respectively. ( J ) Subsequent to fluorescence microscopy, tumor cryosections were stained with H E to get detailed data on tumor tissue, tumor stroma, and adjacent mouse tissue. Abbreviations: b, brain; bl, bladder; gbl, gall bladder; h, heart; in, intestine; li, liver; ln, lymph node; lu, lung; t, tumor; ki, kidney; v, vein.
    Alexa488 Conjugated Goat Anti Rat Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioLegend alexa488 conjugated goat anti rat igg
    Representative images of PET, radioluminography, in vivo fluorescence imaging, and ex vivo (fluorescence) microscopy of A375-pIRES/-EphB4 tumor-bearing NMRI nu/nu mice and tumor cryosections. After intravenous injection [ 18 F]FDG, [ 64 Cu]Cu-ETS, and [ 18 F]FMISO clearly accumulate in A375-pIRES and A375-EphB4 tumors and can be visualized by small animal PET ( A – C ) and subsequent radioluminography ( D – F ). ( A – C ) PET data are illustrated as maximum intensity projection (MIPs) of tracer-dependent frame times ([ 18 F]FDG, 30–60 min p.i.; [ 64 Cu]Cu-ETS, 0–30 min p.i.; [ 18 F]FMISO, 180–240 min p.i.). ( D – F ) Subsequent to PET imaging, A375-pIRES/-EphB4 tumors were resected and distribution of PET tracers was analyzed by radioluminography in tumor cryosections (18 sections each in 3 tumor depth). ( G ) In vivo fluorescence imaging of A375-pIRES/-EphB4 tumor-bearing NMRI nu/nu mice 24 h post i.v. injection of AngioSense (750/790 nm). ( H ) Ex vivo fluorescence microscopy of A375-pIRES/-EphB4 tumor cryosections following i.v. injection of 30 mg/kg Hoechst 33342 exactly 1 min before sacrifice of mice. ( I ) Tumor cryosections were stained with CD31 and LYVE-1 specific primary antibodies as well as <t>Alexa</t> Fluor 488- and Alexa 546-conjugated secondary antibodies to determine blood and lymph vessel markers CD31 (colored in green) and LYVE-1 (colored in orange), respectively. ( J ) Subsequent to fluorescence microscopy, tumor cryosections were stained with H E to get detailed data on tumor tissue, tumor stroma, and adjacent mouse tissue. Abbreviations: b, brain; bl, bladder; gbl, gall bladder; h, heart; in, intestine; li, liver; ln, lymph node; lu, lung; t, tumor; ki, kidney; v, vein.
    Alexa488 Conjugated Goat Anti Rat Igg, supplied by BioLegend, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam goat anti rat alexa 488 conjugated antibody
    Representative images of PET, radioluminography, in vivo fluorescence imaging, and ex vivo (fluorescence) microscopy of A375-pIRES/-EphB4 tumor-bearing NMRI nu/nu mice and tumor cryosections. After intravenous injection [ 18 F]FDG, [ 64 Cu]Cu-ETS, and [ 18 F]FMISO clearly accumulate in A375-pIRES and A375-EphB4 tumors and can be visualized by small animal PET ( A – C ) and subsequent radioluminography ( D – F ). ( A – C ) PET data are illustrated as maximum intensity projection (MIPs) of tracer-dependent frame times ([ 18 F]FDG, 30–60 min p.i.; [ 64 Cu]Cu-ETS, 0–30 min p.i.; [ 18 F]FMISO, 180–240 min p.i.). ( D – F ) Subsequent to PET imaging, A375-pIRES/-EphB4 tumors were resected and distribution of PET tracers was analyzed by radioluminography in tumor cryosections (18 sections each in 3 tumor depth). ( G ) In vivo fluorescence imaging of A375-pIRES/-EphB4 tumor-bearing NMRI nu/nu mice 24 h post i.v. injection of AngioSense (750/790 nm). ( H ) Ex vivo fluorescence microscopy of A375-pIRES/-EphB4 tumor cryosections following i.v. injection of 30 mg/kg Hoechst 33342 exactly 1 min before sacrifice of mice. ( I ) Tumor cryosections were stained with CD31 and LYVE-1 specific primary antibodies as well as <t>Alexa</t> Fluor 488- and Alexa 546-conjugated secondary antibodies to determine blood and lymph vessel markers CD31 (colored in green) and LYVE-1 (colored in orange), respectively. ( J ) Subsequent to fluorescence microscopy, tumor cryosections were stained with H E to get detailed data on tumor tissue, tumor stroma, and adjacent mouse tissue. Abbreviations: b, brain; bl, bladder; gbl, gall bladder; h, heart; in, intestine; li, liver; ln, lymph node; lu, lung; t, tumor; ki, kidney; v, vein.
    Goat Anti Rat Alexa 488 Conjugated Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 96/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam alexa fluor 488 conjugated goat anti rat igg
    Representative images of PET, radioluminography, in vivo fluorescence imaging, and ex vivo (fluorescence) microscopy of A375-pIRES/-EphB4 tumor-bearing NMRI nu/nu mice and tumor cryosections. After intravenous injection [ 18 F]FDG, [ 64 Cu]Cu-ETS, and [ 18 F]FMISO clearly accumulate in A375-pIRES and A375-EphB4 tumors and can be visualized by small animal PET ( A – C ) and subsequent radioluminography ( D – F ). ( A – C ) PET data are illustrated as maximum intensity projection (MIPs) of tracer-dependent frame times ([ 18 F]FDG, 30–60 min p.i.; [ 64 Cu]Cu-ETS, 0–30 min p.i.; [ 18 F]FMISO, 180–240 min p.i.). ( D – F ) Subsequent to PET imaging, A375-pIRES/-EphB4 tumors were resected and distribution of PET tracers was analyzed by radioluminography in tumor cryosections (18 sections each in 3 tumor depth). ( G ) In vivo fluorescence imaging of A375-pIRES/-EphB4 tumor-bearing NMRI nu/nu mice 24 h post i.v. injection of AngioSense (750/790 nm). ( H ) Ex vivo fluorescence microscopy of A375-pIRES/-EphB4 tumor cryosections following i.v. injection of 30 mg/kg Hoechst 33342 exactly 1 min before sacrifice of mice. ( I ) Tumor cryosections were stained with CD31 and LYVE-1 specific primary antibodies as well as <t>Alexa</t> Fluor 488- and Alexa 546-conjugated secondary antibodies to determine blood and lymph vessel markers CD31 (colored in green) and LYVE-1 (colored in orange), respectively. ( J ) Subsequent to fluorescence microscopy, tumor cryosections were stained with H E to get detailed data on tumor tissue, tumor stroma, and adjacent mouse tissue. Abbreviations: b, brain; bl, bladder; gbl, gall bladder; h, heart; in, intestine; li, liver; ln, lymph node; lu, lung; t, tumor; ki, kidney; v, vein.
    Alexa Fluor 488 Conjugated Goat Anti Rat Igg, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher alexa 488 goat anti rat igg conjugate
    Representative images of PET, radioluminography, in vivo fluorescence imaging, and ex vivo (fluorescence) microscopy of A375-pIRES/-EphB4 tumor-bearing NMRI nu/nu mice and tumor cryosections. After intravenous injection [ 18 F]FDG, [ 64 Cu]Cu-ETS, and [ 18 F]FMISO clearly accumulate in A375-pIRES and A375-EphB4 tumors and can be visualized by small animal PET ( A – C ) and subsequent radioluminography ( D – F ). ( A – C ) PET data are illustrated as maximum intensity projection (MIPs) of tracer-dependent frame times ([ 18 F]FDG, 30–60 min p.i.; [ 64 Cu]Cu-ETS, 0–30 min p.i.; [ 18 F]FMISO, 180–240 min p.i.). ( D – F ) Subsequent to PET imaging, A375-pIRES/-EphB4 tumors were resected and distribution of PET tracers was analyzed by radioluminography in tumor cryosections (18 sections each in 3 tumor depth). ( G ) In vivo fluorescence imaging of A375-pIRES/-EphB4 tumor-bearing NMRI nu/nu mice 24 h post i.v. injection of AngioSense (750/790 nm). ( H ) Ex vivo fluorescence microscopy of A375-pIRES/-EphB4 tumor cryosections following i.v. injection of 30 mg/kg Hoechst 33342 exactly 1 min before sacrifice of mice. ( I ) Tumor cryosections were stained with CD31 and LYVE-1 specific primary antibodies as well as <t>Alexa</t> Fluor 488- and Alexa 546-conjugated secondary antibodies to determine blood and lymph vessel markers CD31 (colored in green) and LYVE-1 (colored in orange), respectively. ( J ) Subsequent to fluorescence microscopy, tumor cryosections were stained with H E to get detailed data on tumor tissue, tumor stroma, and adjacent mouse tissue. Abbreviations: b, brain; bl, bladder; gbl, gall bladder; h, heart; in, intestine; li, liver; ln, lymph node; lu, lung; t, tumor; ki, kidney; v, vein.
    Alexa 488 Goat Anti Rat Igg Conjugate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher alexa fluortm 488 goat anti rat igg conjugate
    Representative images of PET, radioluminography, in vivo fluorescence imaging, and ex vivo (fluorescence) microscopy of A375-pIRES/-EphB4 tumor-bearing NMRI nu/nu mice and tumor cryosections. After intravenous injection [ 18 F]FDG, [ 64 Cu]Cu-ETS, and [ 18 F]FMISO clearly accumulate in A375-pIRES and A375-EphB4 tumors and can be visualized by small animal PET ( A – C ) and subsequent radioluminography ( D – F ). ( A – C ) PET data are illustrated as maximum intensity projection (MIPs) of tracer-dependent frame times ([ 18 F]FDG, 30–60 min p.i.; [ 64 Cu]Cu-ETS, 0–30 min p.i.; [ 18 F]FMISO, 180–240 min p.i.). ( D – F ) Subsequent to PET imaging, A375-pIRES/-EphB4 tumors were resected and distribution of PET tracers was analyzed by radioluminography in tumor cryosections (18 sections each in 3 tumor depth). ( G ) In vivo fluorescence imaging of A375-pIRES/-EphB4 tumor-bearing NMRI nu/nu mice 24 h post i.v. injection of AngioSense (750/790 nm). ( H ) Ex vivo fluorescence microscopy of A375-pIRES/-EphB4 tumor cryosections following i.v. injection of 30 mg/kg Hoechst 33342 exactly 1 min before sacrifice of mice. ( I ) Tumor cryosections were stained with CD31 and LYVE-1 specific primary antibodies as well as <t>Alexa</t> Fluor 488- and Alexa 546-conjugated secondary antibodies to determine blood and lymph vessel markers CD31 (colored in green) and LYVE-1 (colored in orange), respectively. ( J ) Subsequent to fluorescence microscopy, tumor cryosections were stained with H E to get detailed data on tumor tissue, tumor stroma, and adjacent mouse tissue. Abbreviations: b, brain; bl, bladder; gbl, gall bladder; h, heart; in, intestine; li, liver; ln, lymph node; lu, lung; t, tumor; ki, kidney; v, vein.
    Alexa Fluortm 488 Goat Anti Rat Igg Conjugate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam alexa 488 conjugated goat anti rat igg
    Representative images of PET, radioluminography, in vivo fluorescence imaging, and ex vivo (fluorescence) microscopy of A375-pIRES/-EphB4 tumor-bearing NMRI nu/nu mice and tumor cryosections. After intravenous injection [ 18 F]FDG, [ 64 Cu]Cu-ETS, and [ 18 F]FMISO clearly accumulate in A375-pIRES and A375-EphB4 tumors and can be visualized by small animal PET ( A – C ) and subsequent radioluminography ( D – F ). ( A – C ) PET data are illustrated as maximum intensity projection (MIPs) of tracer-dependent frame times ([ 18 F]FDG, 30–60 min p.i.; [ 64 Cu]Cu-ETS, 0–30 min p.i.; [ 18 F]FMISO, 180–240 min p.i.). ( D – F ) Subsequent to PET imaging, A375-pIRES/-EphB4 tumors were resected and distribution of PET tracers was analyzed by radioluminography in tumor cryosections (18 sections each in 3 tumor depth). ( G ) In vivo fluorescence imaging of A375-pIRES/-EphB4 tumor-bearing NMRI nu/nu mice 24 h post i.v. injection of AngioSense (750/790 nm). ( H ) Ex vivo fluorescence microscopy of A375-pIRES/-EphB4 tumor cryosections following i.v. injection of 30 mg/kg Hoechst 33342 exactly 1 min before sacrifice of mice. ( I ) Tumor cryosections were stained with CD31 and LYVE-1 specific primary antibodies as well as <t>Alexa</t> Fluor 488- and Alexa 546-conjugated secondary antibodies to determine blood and lymph vessel markers CD31 (colored in green) and LYVE-1 (colored in orange), respectively. ( J ) Subsequent to fluorescence microscopy, tumor cryosections were stained with H E to get detailed data on tumor tissue, tumor stroma, and adjacent mouse tissue. Abbreviations: b, brain; bl, bladder; gbl, gall bladder; h, heart; in, intestine; li, liver; ln, lymph node; lu, lung; t, tumor; ki, kidney; v, vein.
    Alexa 488 Conjugated Goat Anti Rat Igg, supplied by Abcam, used in various techniques. Bioz Stars score: 89/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc alexa 488 conjugated goat anti rat igg
    Representative images of PET, radioluminography, in vivo fluorescence imaging, and ex vivo (fluorescence) microscopy of A375-pIRES/-EphB4 tumor-bearing NMRI nu/nu mice and tumor cryosections. After intravenous injection [ 18 F]FDG, [ 64 Cu]Cu-ETS, and [ 18 F]FMISO clearly accumulate in A375-pIRES and A375-EphB4 tumors and can be visualized by small animal PET ( A – C ) and subsequent radioluminography ( D – F ). ( A – C ) PET data are illustrated as maximum intensity projection (MIPs) of tracer-dependent frame times ([ 18 F]FDG, 30–60 min p.i.; [ 64 Cu]Cu-ETS, 0–30 min p.i.; [ 18 F]FMISO, 180–240 min p.i.). ( D – F ) Subsequent to PET imaging, A375-pIRES/-EphB4 tumors were resected and distribution of PET tracers was analyzed by radioluminography in tumor cryosections (18 sections each in 3 tumor depth). ( G ) In vivo fluorescence imaging of A375-pIRES/-EphB4 tumor-bearing NMRI nu/nu mice 24 h post i.v. injection of AngioSense (750/790 nm). ( H ) Ex vivo fluorescence microscopy of A375-pIRES/-EphB4 tumor cryosections following i.v. injection of 30 mg/kg Hoechst 33342 exactly 1 min before sacrifice of mice. ( I ) Tumor cryosections were stained with CD31 and LYVE-1 specific primary antibodies as well as <t>Alexa</t> Fluor 488- and Alexa 546-conjugated secondary antibodies to determine blood and lymph vessel markers CD31 (colored in green) and LYVE-1 (colored in orange), respectively. ( J ) Subsequent to fluorescence microscopy, tumor cryosections were stained with H E to get detailed data on tumor tissue, tumor stroma, and adjacent mouse tissue. Abbreviations: b, brain; bl, bladder; gbl, gall bladder; h, heart; in, intestine; li, liver; ln, lymph node; lu, lung; t, tumor; ki, kidney; v, vein.
    Alexa 488 Conjugated Goat Anti Rat Igg, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 89/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioLegend alexa fluor conjugated antibodies
    Representative images of PET, radioluminography, in vivo fluorescence imaging, and ex vivo (fluorescence) microscopy of A375-pIRES/-EphB4 tumor-bearing NMRI nu/nu mice and tumor cryosections. After intravenous injection [ 18 F]FDG, [ 64 Cu]Cu-ETS, and [ 18 F]FMISO clearly accumulate in A375-pIRES and A375-EphB4 tumors and can be visualized by small animal PET ( A – C ) and subsequent radioluminography ( D – F ). ( A – C ) PET data are illustrated as maximum intensity projection (MIPs) of tracer-dependent frame times ([ 18 F]FDG, 30–60 min p.i.; [ 64 Cu]Cu-ETS, 0–30 min p.i.; [ 18 F]FMISO, 180–240 min p.i.). ( D – F ) Subsequent to PET imaging, A375-pIRES/-EphB4 tumors were resected and distribution of PET tracers was analyzed by radioluminography in tumor cryosections (18 sections each in 3 tumor depth). ( G ) In vivo fluorescence imaging of A375-pIRES/-EphB4 tumor-bearing NMRI nu/nu mice 24 h post i.v. injection of AngioSense (750/790 nm). ( H ) Ex vivo fluorescence microscopy of A375-pIRES/-EphB4 tumor cryosections following i.v. injection of 30 mg/kg Hoechst 33342 exactly 1 min before sacrifice of mice. ( I ) Tumor cryosections were stained with CD31 and LYVE-1 specific primary antibodies as well as <t>Alexa</t> Fluor 488- and Alexa 546-conjugated secondary antibodies to determine blood and lymph vessel markers CD31 (colored in green) and LYVE-1 (colored in orange), respectively. ( J ) Subsequent to fluorescence microscopy, tumor cryosections were stained with H E to get detailed data on tumor tissue, tumor stroma, and adjacent mouse tissue. Abbreviations: b, brain; bl, bladder; gbl, gall bladder; h, heart; in, intestine; li, liver; ln, lymph node; lu, lung; t, tumor; ki, kidney; v, vein.
    Alexa Fluor Conjugated Antibodies, supplied by BioLegend, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology alexa fluor 488 conjugated goat anti rat igg
    Representative images of PET, radioluminography, in vivo fluorescence imaging, and ex vivo (fluorescence) microscopy of A375-pIRES/-EphB4 tumor-bearing NMRI nu/nu mice and tumor cryosections. After intravenous injection [ 18 F]FDG, [ 64 Cu]Cu-ETS, and [ 18 F]FMISO clearly accumulate in A375-pIRES and A375-EphB4 tumors and can be visualized by small animal PET ( A – C ) and subsequent radioluminography ( D – F ). ( A – C ) PET data are illustrated as maximum intensity projection (MIPs) of tracer-dependent frame times ([ 18 F]FDG, 30–60 min p.i.; [ 64 Cu]Cu-ETS, 0–30 min p.i.; [ 18 F]FMISO, 180–240 min p.i.). ( D – F ) Subsequent to PET imaging, A375-pIRES/-EphB4 tumors were resected and distribution of PET tracers was analyzed by radioluminography in tumor cryosections (18 sections each in 3 tumor depth). ( G ) In vivo fluorescence imaging of A375-pIRES/-EphB4 tumor-bearing NMRI nu/nu mice 24 h post i.v. injection of AngioSense (750/790 nm). ( H ) Ex vivo fluorescence microscopy of A375-pIRES/-EphB4 tumor cryosections following i.v. injection of 30 mg/kg Hoechst 33342 exactly 1 min before sacrifice of mice. ( I ) Tumor cryosections were stained with CD31 and LYVE-1 specific primary antibodies as well as <t>Alexa</t> Fluor 488- and Alexa 546-conjugated secondary antibodies to determine blood and lymph vessel markers CD31 (colored in green) and LYVE-1 (colored in orange), respectively. ( J ) Subsequent to fluorescence microscopy, tumor cryosections were stained with H E to get detailed data on tumor tissue, tumor stroma, and adjacent mouse tissue. Abbreviations: b, brain; bl, bladder; gbl, gall bladder; h, heart; in, intestine; li, liver; ln, lymph node; lu, lung; t, tumor; ki, kidney; v, vein.
    Alexa Fluor 488 Conjugated Goat Anti Rat Igg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Beyotime alexa fluor 488 conjugated goat anti rat igg
    Representative images of PET, radioluminography, in vivo fluorescence imaging, and ex vivo (fluorescence) microscopy of A375-pIRES/-EphB4 tumor-bearing NMRI nu/nu mice and tumor cryosections. After intravenous injection [ 18 F]FDG, [ 64 Cu]Cu-ETS, and [ 18 F]FMISO clearly accumulate in A375-pIRES and A375-EphB4 tumors and can be visualized by small animal PET ( A – C ) and subsequent radioluminography ( D – F ). ( A – C ) PET data are illustrated as maximum intensity projection (MIPs) of tracer-dependent frame times ([ 18 F]FDG, 30–60 min p.i.; [ 64 Cu]Cu-ETS, 0–30 min p.i.; [ 18 F]FMISO, 180–240 min p.i.). ( D – F ) Subsequent to PET imaging, A375-pIRES/-EphB4 tumors were resected and distribution of PET tracers was analyzed by radioluminography in tumor cryosections (18 sections each in 3 tumor depth). ( G ) In vivo fluorescence imaging of A375-pIRES/-EphB4 tumor-bearing NMRI nu/nu mice 24 h post i.v. injection of AngioSense (750/790 nm). ( H ) Ex vivo fluorescence microscopy of A375-pIRES/-EphB4 tumor cryosections following i.v. injection of 30 mg/kg Hoechst 33342 exactly 1 min before sacrifice of mice. ( I ) Tumor cryosections were stained with CD31 and LYVE-1 specific primary antibodies as well as <t>Alexa</t> Fluor 488- and Alexa 546-conjugated secondary antibodies to determine blood and lymph vessel markers CD31 (colored in green) and LYVE-1 (colored in orange), respectively. ( J ) Subsequent to fluorescence microscopy, tumor cryosections were stained with H E to get detailed data on tumor tissue, tumor stroma, and adjacent mouse tissue. Abbreviations: b, brain; bl, bladder; gbl, gall bladder; h, heart; in, intestine; li, liver; ln, lymph node; lu, lung; t, tumor; ki, kidney; v, vein.
    Alexa Fluor 488 Conjugated Goat Anti Rat Igg, supplied by Beyotime, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Representative images of PET, radioluminography, in vivo fluorescence imaging, and ex vivo (fluorescence) microscopy of A375-pIRES/-EphB4 tumor-bearing NMRI nu/nu mice and tumor cryosections. After intravenous injection [ 18 F]FDG, [ 64 Cu]Cu-ETS, and [ 18 F]FMISO clearly accumulate in A375-pIRES and A375-EphB4 tumors and can be visualized by small animal PET ( A – C ) and subsequent radioluminography ( D – F ). ( A – C ) PET data are illustrated as maximum intensity projection (MIPs) of tracer-dependent frame times ([ 18 F]FDG, 30–60 min p.i.; [ 64 Cu]Cu-ETS, 0–30 min p.i.; [ 18 F]FMISO, 180–240 min p.i.). ( D – F ) Subsequent to PET imaging, A375-pIRES/-EphB4 tumors were resected and distribution of PET tracers was analyzed by radioluminography in tumor cryosections (18 sections each in 3 tumor depth). ( G ) In vivo fluorescence imaging of A375-pIRES/-EphB4 tumor-bearing NMRI nu/nu mice 24 h post i.v. injection of AngioSense (750/790 nm). ( H ) Ex vivo fluorescence microscopy of A375-pIRES/-EphB4 tumor cryosections following i.v. injection of 30 mg/kg Hoechst 33342 exactly 1 min before sacrifice of mice. ( I ) Tumor cryosections were stained with CD31 and LYVE-1 specific primary antibodies as well as Alexa Fluor 488- and Alexa 546-conjugated secondary antibodies to determine blood and lymph vessel markers CD31 (colored in green) and LYVE-1 (colored in orange), respectively. ( J ) Subsequent to fluorescence microscopy, tumor cryosections were stained with H E to get detailed data on tumor tissue, tumor stroma, and adjacent mouse tissue. Abbreviations: b, brain; bl, bladder; gbl, gall bladder; h, heart; in, intestine; li, liver; ln, lymph node; lu, lung; t, tumor; ki, kidney; v, vein.

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: Overexpression of Receptor Tyrosine Kinase EphB4 Triggers Tumor Growth and Hypoxia in A375 Melanoma Xenografts: Insights from Multitracer Small Animal Imaging Experiments ‡

    doi: 10.3390/molecules23020444

    Figure Lengend Snippet: Representative images of PET, radioluminography, in vivo fluorescence imaging, and ex vivo (fluorescence) microscopy of A375-pIRES/-EphB4 tumor-bearing NMRI nu/nu mice and tumor cryosections. After intravenous injection [ 18 F]FDG, [ 64 Cu]Cu-ETS, and [ 18 F]FMISO clearly accumulate in A375-pIRES and A375-EphB4 tumors and can be visualized by small animal PET ( A – C ) and subsequent radioluminography ( D – F ). ( A – C ) PET data are illustrated as maximum intensity projection (MIPs) of tracer-dependent frame times ([ 18 F]FDG, 30–60 min p.i.; [ 64 Cu]Cu-ETS, 0–30 min p.i.; [ 18 F]FMISO, 180–240 min p.i.). ( D – F ) Subsequent to PET imaging, A375-pIRES/-EphB4 tumors were resected and distribution of PET tracers was analyzed by radioluminography in tumor cryosections (18 sections each in 3 tumor depth). ( G ) In vivo fluorescence imaging of A375-pIRES/-EphB4 tumor-bearing NMRI nu/nu mice 24 h post i.v. injection of AngioSense (750/790 nm). ( H ) Ex vivo fluorescence microscopy of A375-pIRES/-EphB4 tumor cryosections following i.v. injection of 30 mg/kg Hoechst 33342 exactly 1 min before sacrifice of mice. ( I ) Tumor cryosections were stained with CD31 and LYVE-1 specific primary antibodies as well as Alexa Fluor 488- and Alexa 546-conjugated secondary antibodies to determine blood and lymph vessel markers CD31 (colored in green) and LYVE-1 (colored in orange), respectively. ( J ) Subsequent to fluorescence microscopy, tumor cryosections were stained with H E to get detailed data on tumor tissue, tumor stroma, and adjacent mouse tissue. Abbreviations: b, brain; bl, bladder; gbl, gall bladder; h, heart; in, intestine; li, liver; ln, lymph node; lu, lung; t, tumor; ki, kidney; v, vein.

    Article Snippet: Primary antibodies for CD31 and LYVE 1 were visualized by Alexa Fluor 488-conjugated goat anti-rat IgG (Thermo Fisher Scientific, Langenselbold, Germany, ; 10 µg/mL) and Alexa 546-conjugated donkey anti rabbit IgG (Thermo Fisher Scientific, , 10 µg/mL, LYVE-1), respectively, for 1 h at room temperature and subsequent fluorescence microscopy using the microscope AxioImager.A1 (Zeiss, Jena, Germany) and software AxioVision (Zeiss).

    Techniques: Positron Emission Tomography, In Vivo, Fluorescence, Imaging, Ex Vivo, Microscopy, Mouse Assay, Injection, Staining

    Effect of prolonged depolymerization or stabilization of CMT arrays on the formation of the ingrowth wall labyrinth in adaxial epidermal cells of V. faba cotyledons. Confocal images of immunolabelling with anti-α-tubulin and IgG–Alexa Fluor 488 conjugate (A, C, E) and SEM images of the cytoplasmic face of the ingrowth wall labyrinth (B, D, F) of epidermal cells of cotyledons cultured for 3 d on MS medium alone (A, B) or in the presence of 20 µM oryzalin (C, D) or 5 µM taxol (E, F). Note that in the control (A), the fluorescence is diffuse and there are some depletion zones (arrowheads). In oryzalin-treated cells (C), the tubulin fluorescence appeared to be aggregated in places, with depletion zones (arrowheads) visible, while in cells of the taxol-treated cotyledons (E), thick microtubule bundles were aligned perpendicular to their longitudinal axis. The ingrowth walls in (B), (D), and (F) were identical, being comprised of three fenestrated layers (number of layers labelled in inserts of B, D, F) with wall ingrowth papillae (arrowheads) arising from the most recently deposited fenestrated layer. Bar, 10 µm (A–F); 2 µm, inserts.

    Journal: Journal of Experimental Botany

    Article Title: Calcium-dependent depletion zones in the cortical microtubule array coincide with sites of, but do not regulate, wall ingrowth papillae deposition in epidermal transfer cells

    doi: 10.1093/jxb/erv317

    Figure Lengend Snippet: Effect of prolonged depolymerization or stabilization of CMT arrays on the formation of the ingrowth wall labyrinth in adaxial epidermal cells of V. faba cotyledons. Confocal images of immunolabelling with anti-α-tubulin and IgG–Alexa Fluor 488 conjugate (A, C, E) and SEM images of the cytoplasmic face of the ingrowth wall labyrinth (B, D, F) of epidermal cells of cotyledons cultured for 3 d on MS medium alone (A, B) or in the presence of 20 µM oryzalin (C, D) or 5 µM taxol (E, F). Note that in the control (A), the fluorescence is diffuse and there are some depletion zones (arrowheads). In oryzalin-treated cells (C), the tubulin fluorescence appeared to be aggregated in places, with depletion zones (arrowheads) visible, while in cells of the taxol-treated cotyledons (E), thick microtubule bundles were aligned perpendicular to their longitudinal axis. The ingrowth walls in (B), (D), and (F) were identical, being comprised of three fenestrated layers (number of layers labelled in inserts of B, D, F) with wall ingrowth papillae (arrowheads) arising from the most recently deposited fenestrated layer. Bar, 10 µm (A–F); 2 µm, inserts.

    Article Snippet: After blocking, peels were incubated overnight at 4 °C with a mouse monoclonal anti-α-tubulin antibody (Sigma-Aldrich, USA), diluted 1:100 in PBS/BSA, washed six times for 10min each in PBS with 0.2% (v/v) Tween 20 (PBS/T) and incubated for a further 3h at room temperature in secondary antibody, goat anti-rat IgG conjugated to Alexa Fluor 488 (Molecular Probes, Eugene, OR, USA) diluted 1:100 in PBS/T.

    Techniques: Cell Culture, Mass Spectrometry, Fluorescence

    Effect of oryzalin and taxol on CMT array and wall ingrowth papillae formation in adaxial epidermal cells of cultured V. faba cotyledons. Confocal images of CMTs immunolabelled with anti-α-tubulin and IgG–Alexa Fluor 488 conjugate (A, C, E) and SEM images of the cytoplasmic face of the outer periclinal wall to visualize wall ingrowth papillae (B, D, F) of epidermal cells of cotyledons cultured for 24h in MS medium alone (A, B) or 24h in the presence of, 20 µM oryzalin (C, D) or 5 µM taxol (E, F). In the presence of oryzalin, CMT arrays were depolymerized and then tubulin aggregated and exhibited depletion zones (arrowheads in C). In contrast, CMT arrays were stabilized in the presence of taxol (E) retaining an alignment comparable to those located in freshly harvested cotyledons (c.f. Fig. 2A ). Bar, 10 µm.

    Journal: Journal of Experimental Botany

    Article Title: Calcium-dependent depletion zones in the cortical microtubule array coincide with sites of, but do not regulate, wall ingrowth papillae deposition in epidermal transfer cells

    doi: 10.1093/jxb/erv317

    Figure Lengend Snippet: Effect of oryzalin and taxol on CMT array and wall ingrowth papillae formation in adaxial epidermal cells of cultured V. faba cotyledons. Confocal images of CMTs immunolabelled with anti-α-tubulin and IgG–Alexa Fluor 488 conjugate (A, C, E) and SEM images of the cytoplasmic face of the outer periclinal wall to visualize wall ingrowth papillae (B, D, F) of epidermal cells of cotyledons cultured for 24h in MS medium alone (A, B) or 24h in the presence of, 20 µM oryzalin (C, D) or 5 µM taxol (E, F). In the presence of oryzalin, CMT arrays were depolymerized and then tubulin aggregated and exhibited depletion zones (arrowheads in C). In contrast, CMT arrays were stabilized in the presence of taxol (E) retaining an alignment comparable to those located in freshly harvested cotyledons (c.f. Fig. 2A ). Bar, 10 µm.

    Article Snippet: After blocking, peels were incubated overnight at 4 °C with a mouse monoclonal anti-α-tubulin antibody (Sigma-Aldrich, USA), diluted 1:100 in PBS/BSA, washed six times for 10min each in PBS with 0.2% (v/v) Tween 20 (PBS/T) and incubated for a further 3h at room temperature in secondary antibody, goat anti-rat IgG conjugated to Alexa Fluor 488 (Molecular Probes, Eugene, OR, USA) diluted 1:100 in PBS/T.

    Techniques: Cell Culture, Mass Spectrometry

    Confocal images of three categories of CMT arrays observed in adaxial epidermal cells of V. faba cotyledons cultured for 24h. CMT arrays immunolabelled with anti-α-tubulin and IgG–Alexa Fluor 488 conjugate. (A) ‘Organized’: parallel arrays of thick CMT bundles. (B) ‘Randomized’: an array defined by thick, strongly labelled CMT bundles arranged in a random network with distinctive polygonal gaps (arrowheads) in the network. (C) ‘Randomized with depletion zones’: an array composed of circular depletion zones surrounded by a possible combination of fine fragmented CMTs and tubulin monomers sometimes appearing like a collar (arrowheads). Bar, 2.5 μm.

    Journal: Journal of Experimental Botany

    Article Title: Calcium-dependent depletion zones in the cortical microtubule array coincide with sites of, but do not regulate, wall ingrowth papillae deposition in epidermal transfer cells

    doi: 10.1093/jxb/erv317

    Figure Lengend Snippet: Confocal images of three categories of CMT arrays observed in adaxial epidermal cells of V. faba cotyledons cultured for 24h. CMT arrays immunolabelled with anti-α-tubulin and IgG–Alexa Fluor 488 conjugate. (A) ‘Organized’: parallel arrays of thick CMT bundles. (B) ‘Randomized’: an array defined by thick, strongly labelled CMT bundles arranged in a random network with distinctive polygonal gaps (arrowheads) in the network. (C) ‘Randomized with depletion zones’: an array composed of circular depletion zones surrounded by a possible combination of fine fragmented CMTs and tubulin monomers sometimes appearing like a collar (arrowheads). Bar, 2.5 μm.

    Article Snippet: After blocking, peels were incubated overnight at 4 °C with a mouse monoclonal anti-α-tubulin antibody (Sigma-Aldrich, USA), diluted 1:100 in PBS/BSA, washed six times for 10min each in PBS with 0.2% (v/v) Tween 20 (PBS/T) and incubated for a further 3h at room temperature in secondary antibody, goat anti-rat IgG conjugated to Alexa Fluor 488 (Molecular Probes, Eugene, OR, USA) diluted 1:100 in PBS/T.

    Techniques: Cell Culture

    Changes in CMT organization during wall ingrowth papillae formation in adaxial epidermal cells of V. faba cotyledons cultured for 24h. (A–D) Adaxial epidermal peels from freshly harvested (0h) (A, B) or cotyledons cultured for 24h (C, D). Wall ingrowth papillae were visualized by viewing the cytoplasmic face of the outer periclinal wall of cells by SEM (A, C) or epidermal peels were immunolabelled with anti-α-tubulin and IgG–Alexa Fluor 488 conjugate to visualize CMT organization by CLSM (B, D). In freshly harvested cotyledons, CMT arrays (B, arrowheads) in their adaxial epidermal cells were mostly aligned in parallel arrays either transverse to the long (L) or short (T) axis of each epidermal cell, or in an oblique pattern to the long axis (O) (see E). In a small number of cells, the CMT array was organized randomly (R). After 24h of cotyledon culture, in which wall ingrowth papillae had been deposited in most cells (C, arrowheads), CMTs were randomly organized (D) (see E). Bar, 20 μm. (E) Angles of CMTs relative to the long axis of the cell expressed as the percentage frequency of total CMT angles measured.

    Journal: Journal of Experimental Botany

    Article Title: Calcium-dependent depletion zones in the cortical microtubule array coincide with sites of, but do not regulate, wall ingrowth papillae deposition in epidermal transfer cells

    doi: 10.1093/jxb/erv317

    Figure Lengend Snippet: Changes in CMT organization during wall ingrowth papillae formation in adaxial epidermal cells of V. faba cotyledons cultured for 24h. (A–D) Adaxial epidermal peels from freshly harvested (0h) (A, B) or cotyledons cultured for 24h (C, D). Wall ingrowth papillae were visualized by viewing the cytoplasmic face of the outer periclinal wall of cells by SEM (A, C) or epidermal peels were immunolabelled with anti-α-tubulin and IgG–Alexa Fluor 488 conjugate to visualize CMT organization by CLSM (B, D). In freshly harvested cotyledons, CMT arrays (B, arrowheads) in their adaxial epidermal cells were mostly aligned in parallel arrays either transverse to the long (L) or short (T) axis of each epidermal cell, or in an oblique pattern to the long axis (O) (see E). In a small number of cells, the CMT array was organized randomly (R). After 24h of cotyledon culture, in which wall ingrowth papillae had been deposited in most cells (C, arrowheads), CMTs were randomly organized (D) (see E). Bar, 20 μm. (E) Angles of CMTs relative to the long axis of the cell expressed as the percentage frequency of total CMT angles measured.

    Article Snippet: After blocking, peels were incubated overnight at 4 °C with a mouse monoclonal anti-α-tubulin antibody (Sigma-Aldrich, USA), diluted 1:100 in PBS/BSA, washed six times for 10min each in PBS with 0.2% (v/v) Tween 20 (PBS/T) and incubated for a further 3h at room temperature in secondary antibody, goat anti-rat IgG conjugated to Alexa Fluor 488 (Molecular Probes, Eugene, OR, USA) diluted 1:100 in PBS/T.

    Techniques: Cell Culture, Confocal Laser Scanning Microscopy

    Temporal and spatial relationships between categories of CMT arrays and wall ingrowth papillae in adaxial epidermal cells of cultured V. faba cotyledons. (A) Temporal pattern of changes in the percentages of cells exhibiting organized (squares), randomized (triangles), and randomized with depletion zones (circles) CMT arrays across 24h of cotyledon culture. Data are means±SE from four replicate cotyledons with a minimum of 100 cells recorded for each cotyledon. Data from Wardini et al. (2007) on the percentage of epidermal cells with wall ingrowth papillae is superimposed (blue line). (B) Spatial relationship between circular depletion zones in the ‘randomized with depletion zones’ CMT array and wall ingrowth papillae in cells of cotyledons cultured for 24h. (I) CMTs immunolabelled with anti-α-tubulin and IgG–Alexa Fluor 488 conjugate. (II) Cell walls stained with Congo red. (III) Digital overlay of labelling of CMT array and cell wall staining. Circular depletion zones (I) intensely stained wall ingrowth papillae (II) and their co-localization (III) indicated by arrowheads. Arrows indicate shorter wall ingrowth papillae and their overarching CMTs. Bar, 2 μm.

    Journal: Journal of Experimental Botany

    Article Title: Calcium-dependent depletion zones in the cortical microtubule array coincide with sites of, but do not regulate, wall ingrowth papillae deposition in epidermal transfer cells

    doi: 10.1093/jxb/erv317

    Figure Lengend Snippet: Temporal and spatial relationships between categories of CMT arrays and wall ingrowth papillae in adaxial epidermal cells of cultured V. faba cotyledons. (A) Temporal pattern of changes in the percentages of cells exhibiting organized (squares), randomized (triangles), and randomized with depletion zones (circles) CMT arrays across 24h of cotyledon culture. Data are means±SE from four replicate cotyledons with a minimum of 100 cells recorded for each cotyledon. Data from Wardini et al. (2007) on the percentage of epidermal cells with wall ingrowth papillae is superimposed (blue line). (B) Spatial relationship between circular depletion zones in the ‘randomized with depletion zones’ CMT array and wall ingrowth papillae in cells of cotyledons cultured for 24h. (I) CMTs immunolabelled with anti-α-tubulin and IgG–Alexa Fluor 488 conjugate. (II) Cell walls stained with Congo red. (III) Digital overlay of labelling of CMT array and cell wall staining. Circular depletion zones (I) intensely stained wall ingrowth papillae (II) and their co-localization (III) indicated by arrowheads. Arrows indicate shorter wall ingrowth papillae and their overarching CMTs. Bar, 2 μm.

    Article Snippet: After blocking, peels were incubated overnight at 4 °C with a mouse monoclonal anti-α-tubulin antibody (Sigma-Aldrich, USA), diluted 1:100 in PBS/BSA, washed six times for 10min each in PBS with 0.2% (v/v) Tween 20 (PBS/T) and incubated for a further 3h at room temperature in secondary antibody, goat anti-rat IgG conjugated to Alexa Fluor 488 (Molecular Probes, Eugene, OR, USA) diluted 1:100 in PBS/T.

    Techniques: Cell Culture, Staining

    Effect of manipulating wall ingrowth papillae deposition and cytosolic Ca 2+ on reorganization of CMT arrays in adaxial epidermal cells of cultured V. faba cotyledons. Representative SEM images of the cytoplasmic face of the outer periclinal wall (A, C, E) and confocal images of CMTs immunolabelled with anti-α-tubulin and IgG–Alexa Fluor 488 conjugate (B, D, F) of cells of cotyledons cultured for 24h in the presence of 600 µM of the Ca 2+ chelator BAPTA (A, B), 5 µM of the cellulose biosynthesis inhibitor DCB (C, D) or 0.5 µM of the Ca 2+ ATPase inhibitor Eosin Yellow (E, F). In the presence of BAPTA, wall ingrowth papillae formation was inhibited (A) and the CMT arrays, while randomized, were devoid of localized depletion zones (B). In contrast, when wall ingrowth papillae formation was inhibited in the presence of DCB (C), randomized CMT arrays exhibited circular depletion zones (D, arrowheads). Dissipation of localized Ca 2+ plumes with Eosin Yellow resulted in a partially, but uniformly, depleted CMT array (F) and inhibition of wall ingrowth papillae formation (E). Bar, 5 µm.

    Journal: Journal of Experimental Botany

    Article Title: Calcium-dependent depletion zones in the cortical microtubule array coincide with sites of, but do not regulate, wall ingrowth papillae deposition in epidermal transfer cells

    doi: 10.1093/jxb/erv317

    Figure Lengend Snippet: Effect of manipulating wall ingrowth papillae deposition and cytosolic Ca 2+ on reorganization of CMT arrays in adaxial epidermal cells of cultured V. faba cotyledons. Representative SEM images of the cytoplasmic face of the outer periclinal wall (A, C, E) and confocal images of CMTs immunolabelled with anti-α-tubulin and IgG–Alexa Fluor 488 conjugate (B, D, F) of cells of cotyledons cultured for 24h in the presence of 600 µM of the Ca 2+ chelator BAPTA (A, B), 5 µM of the cellulose biosynthesis inhibitor DCB (C, D) or 0.5 µM of the Ca 2+ ATPase inhibitor Eosin Yellow (E, F). In the presence of BAPTA, wall ingrowth papillae formation was inhibited (A) and the CMT arrays, while randomized, were devoid of localized depletion zones (B). In contrast, when wall ingrowth papillae formation was inhibited in the presence of DCB (C), randomized CMT arrays exhibited circular depletion zones (D, arrowheads). Dissipation of localized Ca 2+ plumes with Eosin Yellow resulted in a partially, but uniformly, depleted CMT array (F) and inhibition of wall ingrowth papillae formation (E). Bar, 5 µm.

    Article Snippet: After blocking, peels were incubated overnight at 4 °C with a mouse monoclonal anti-α-tubulin antibody (Sigma-Aldrich, USA), diluted 1:100 in PBS/BSA, washed six times for 10min each in PBS with 0.2% (v/v) Tween 20 (PBS/T) and incubated for a further 3h at room temperature in secondary antibody, goat anti-rat IgG conjugated to Alexa Fluor 488 (Molecular Probes, Eugene, OR, USA) diluted 1:100 in PBS/T.

    Techniques: Cell Culture, Inhibition