Journal: Journal of Virology
Article Title: Hepatitis E Virus Genotype 1 Infection of Swine Kidney Cells In Vitro Is Inhibited at Multiple Levels
Figure Lengend Snippet: Flow cytometry of transfected cells stained separately for the ORF2 or ORF3 protein. HepG2/C3A and LLC-PK cells electroporated with transcripts from P6 (A), Sar55 (B), or Sar/S17 (C, D) were plated in 6 wells of a 6-well culture plate, incubated at 34.5°C, and harvested 7 (A, B) or 5 (C, D) days later. Cells in triplicate wells were stained for the ORF2 protein, followed by goat anti-human IgG labeled with Alexa Fluor 488 (open bars); cells in the other 3 wells were stained for the ORF3 protein, followed by goat anti-rabbit IgG also labeled with Alexa Fluor 488 (hatched bars). Flow cytometry was performed with the same settings for all samples. (A to C) Mean percentage of positive cells, in triplicate; (D) mean of the geometric mean fluorescence intensity in the same triplicate samples assayed for panel C. Shaded bars, ORF2; dotted bars, ORF3. Error bars indicate standard deviations. P values were determined by Student's t test; P values of less than 0.05 were statistically significant.
Article Snippet: Secondary antibodies were a mixture of Alexa Fluor 488-conjugated goat anti-human IgG (Molecular Probes) and Alexa Fluor 568-conjugated goat anti-rabbit IgG (Molecular Probes).
Techniques: Flow Cytometry, Cytometry, Transfection, Staining, Incubation, Labeling, Fluorescence