alexa fluor 488 goat anti-rat igg h+l Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher goat anti rat igg h l cross adsorbed secondary antibody
    Goat Anti Rat Igg H L Cross Adsorbed Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3672 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti rat igg h l cross adsorbed secondary antibody/product/Thermo Fisher
    Average 99 stars, based on 3672 article reviews
    Price from $9.99 to $1999.99
    goat anti rat igg h l cross adsorbed secondary antibody - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Abcam goat anti rat igg h l alexa fluor 488
    Goat Anti Rat Igg H L Alexa Fluor 488, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti rat igg h l alexa fluor 488/product/Abcam
    Average 99 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    goat anti rat igg h l alexa fluor 488 - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    90
    Thermo Fisher alexa fluor 488 conjugated goat anti rat igg
    Alexa Fluor 488 Conjugated Goat Anti Rat Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 372 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor 488 conjugated goat anti rat igg/product/Thermo Fisher
    Average 90 stars, based on 372 article reviews
    Price from $9.99 to $1999.99
    alexa fluor 488 conjugated goat anti rat igg - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    94
    Abcam secondary antibodies
    Secondary Antibodies, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 1440 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/secondary antibodies/product/Abcam
    Average 94 stars, based on 1440 article reviews
    Price from $9.99 to $1999.99
    secondary antibodies - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    99
    Abcam alexa fluor 488 conjugated secondary antibodies
    Confocal laser scanning microscopy utilization to visualize cytoplasmic delivery of P6 NPs. (a) Dil-P6 NPs were incubated with A2780cis cells in the presence of <t>Alexa-Fluor</t> 488-labeled markers of various endocytic pathways. NPs co-localized with dextran, a fluid-phase marker known to enter cells via macropinocytosis (white arrows). (b) Alexa-Fluor 488-labeled actin fibers revealed membrane ruffling and actin rearrangement (white arrows), hallmarks of uptake by macropinocytosis.
    Alexa Fluor 488 Conjugated Secondary Antibodies, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor 488 conjugated secondary antibodies/product/Abcam
    Average 99 stars, based on 63 article reviews
    Price from $9.99 to $1999.99
    alexa fluor 488 conjugated secondary antibodies - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher alexa fluor 488
    Confocal laser scanning microscopy utilization to visualize cytoplasmic delivery of P6 NPs. (a) Dil-P6 NPs were incubated with A2780cis cells in the presence of <t>Alexa-Fluor</t> 488-labeled markers of various endocytic pathways. NPs co-localized with dextran, a fluid-phase marker known to enter cells via macropinocytosis (white arrows). (b) Alexa-Fluor 488-labeled actin fibers revealed membrane ruffling and actin rearrangement (white arrows), hallmarks of uptake by macropinocytosis.
    Alexa Fluor 488, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 71269 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor 488/product/Thermo Fisher
    Average 99 stars, based on 71269 article reviews
    Price from $9.99 to $1999.99
    alexa fluor 488 - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Abcam goat anti mouse igg h l alexa fluor 488 preadsorbed
    Confocal laser scanning microscopy utilization to visualize cytoplasmic delivery of P6 NPs. (a) Dil-P6 NPs were incubated with A2780cis cells in the presence of <t>Alexa-Fluor</t> 488-labeled markers of various endocytic pathways. NPs co-localized with dextran, a fluid-phase marker known to enter cells via macropinocytosis (white arrows). (b) Alexa-Fluor 488-labeled actin fibers revealed membrane ruffling and actin rearrangement (white arrows), hallmarks of uptake by macropinocytosis.
    Goat Anti Mouse Igg H L Alexa Fluor 488 Preadsorbed, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 104 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse igg h l alexa fluor 488 preadsorbed/product/Abcam
    Average 99 stars, based on 104 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse igg h l alexa fluor 488 preadsorbed - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    89
    Abcam goat anti rat igg h l
    Confocal laser scanning microscopy utilization to visualize cytoplasmic delivery of P6 NPs. (a) Dil-P6 NPs were incubated with A2780cis cells in the presence of <t>Alexa-Fluor</t> 488-labeled markers of various endocytic pathways. NPs co-localized with dextran, a fluid-phase marker known to enter cells via macropinocytosis (white arrows). (b) Alexa-Fluor 488-labeled actin fibers revealed membrane ruffling and actin rearrangement (white arrows), hallmarks of uptake by macropinocytosis.
    Goat Anti Rat Igg H L, supplied by Abcam, used in various techniques. Bioz Stars score: 89/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti rat igg h l/product/Abcam
    Average 89 stars, based on 95 article reviews
    Price from $9.99 to $1999.99
    goat anti rat igg h l - by Bioz Stars, 2020-09
    89/100 stars
      Buy from Supplier

    99
    Abcam anti goat igg h l
    Confocal laser scanning microscopy utilization to visualize cytoplasmic delivery of P6 NPs. (a) Dil-P6 NPs were incubated with A2780cis cells in the presence of <t>Alexa-Fluor</t> 488-labeled markers of various endocytic pathways. NPs co-localized with dextran, a fluid-phase marker known to enter cells via macropinocytosis (white arrows). (b) Alexa-Fluor 488-labeled actin fibers revealed membrane ruffling and actin rearrangement (white arrows), hallmarks of uptake by macropinocytosis.
    Anti Goat Igg H L, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti goat igg h l/product/Abcam
    Average 99 stars, based on 44 article reviews
    Price from $9.99 to $1999.99
    anti goat igg h l - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc anti rat igg h l alexa fluor 488 conjugate
    Pristimerin treatment decreases Shh-induced pericyte motilities of angiogenesis. a Pristimerin suppresses Shh-mediated adhesion ability of endothelial cells. The cells that pre-treated with Shh (100 ng/mL) and pristimerin (125, 250, and 500 nM) were seeded at 24-well plate. After 1-h culture, the un-attached cells were removed and the attached cells were stained with 0.1% crystal violet. b Pristimerin restrains Shh-induced pericyte migration to newly formed networks in a three-dimensional co-culture system of HUVEC and HBVP. HUVECs (red) were seeded on Matrigel-coated 96-well and cultured for 2 h to allow the formation of tube network. And HBVPs (green) pre-treated with or without pristimerin were added to the endothelial tubes. The recruitment of HBVPs to HUVEC tubes were observed and photographed at 0, 2, and 12 h. Scale bar: 20 μm. c Pristimerin suppresses the nucleus translocation of Gli1in HBVPs. HBVPs were stimulated with Shh (100 ng/mL) and pristimerin for 24 h. After staining with 4% paraformaldehyde, the HBVPs were labeled with Gli1 antibody and <t>Alexa</t> <t>Fluor</t> 488 Donkey anti-Rabbit <t>IgG.</t> DAPI was used to recognize the nucleus, and the images were taken with a laser scanning confocal microscope. d Pristimerin inhibits the distribution of Gli1 in the nucleus. HBVPs were collected and lysed with a NE-PERTM Nucleus and Cytoplasmic Extraction Kit following the manufacturer’s protocol, and then applied for western blotting assay. β-actin and Lamin B were set as loading control. e Pristimerin blocks the downstream of Shh/Gli1 signaling pathway. After being treated with Shh and pristimerin for 24 h, HBVP cells were lysed using RIPA buffer and subjected to western blotting assay. β-actin was set as loading control. The data are presented as mean ± SEM, n = 3. *** P
    Anti Rat Igg H L Alexa Fluor 488 Conjugate, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti rat igg h l alexa fluor 488 conjugate/product/Cell Signaling Technology Inc
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    anti rat igg h l alexa fluor 488 conjugate - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Abcam alexa 647 conjugated secondary antibodies
    Pristimerin treatment decreases Shh-induced pericyte motilities of angiogenesis. a Pristimerin suppresses Shh-mediated adhesion ability of endothelial cells. The cells that pre-treated with Shh (100 ng/mL) and pristimerin (125, 250, and 500 nM) were seeded at 24-well plate. After 1-h culture, the un-attached cells were removed and the attached cells were stained with 0.1% crystal violet. b Pristimerin restrains Shh-induced pericyte migration to newly formed networks in a three-dimensional co-culture system of HUVEC and HBVP. HUVECs (red) were seeded on Matrigel-coated 96-well and cultured for 2 h to allow the formation of tube network. And HBVPs (green) pre-treated with or without pristimerin were added to the endothelial tubes. The recruitment of HBVPs to HUVEC tubes were observed and photographed at 0, 2, and 12 h. Scale bar: 20 μm. c Pristimerin suppresses the nucleus translocation of Gli1in HBVPs. HBVPs were stimulated with Shh (100 ng/mL) and pristimerin for 24 h. After staining with 4% paraformaldehyde, the HBVPs were labeled with Gli1 antibody and <t>Alexa</t> <t>Fluor</t> 488 Donkey anti-Rabbit <t>IgG.</t> DAPI was used to recognize the nucleus, and the images were taken with a laser scanning confocal microscope. d Pristimerin inhibits the distribution of Gli1 in the nucleus. HBVPs were collected and lysed with a NE-PERTM Nucleus and Cytoplasmic Extraction Kit following the manufacturer’s protocol, and then applied for western blotting assay. β-actin and Lamin B were set as loading control. e Pristimerin blocks the downstream of Shh/Gli1 signaling pathway. After being treated with Shh and pristimerin for 24 h, HBVP cells were lysed using RIPA buffer and subjected to western blotting assay. β-actin was set as loading control. The data are presented as mean ± SEM, n = 3. *** P
    Alexa 647 Conjugated Secondary Antibodies, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa 647 conjugated secondary antibodies/product/Abcam
    Average 99 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    alexa 647 conjugated secondary antibodies - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    94
    Abcam alexa fluor 488 conjugated goat anti rat igg
    Pristimerin treatment decreases Shh-induced pericyte motilities of angiogenesis. a Pristimerin suppresses Shh-mediated adhesion ability of endothelial cells. The cells that pre-treated with Shh (100 ng/mL) and pristimerin (125, 250, and 500 nM) were seeded at 24-well plate. After 1-h culture, the un-attached cells were removed and the attached cells were stained with 0.1% crystal violet. b Pristimerin restrains Shh-induced pericyte migration to newly formed networks in a three-dimensional co-culture system of HUVEC and HBVP. HUVECs (red) were seeded on Matrigel-coated 96-well and cultured for 2 h to allow the formation of tube network. And HBVPs (green) pre-treated with or without pristimerin were added to the endothelial tubes. The recruitment of HBVPs to HUVEC tubes were observed and photographed at 0, 2, and 12 h. Scale bar: 20 μm. c Pristimerin suppresses the nucleus translocation of Gli1in HBVPs. HBVPs were stimulated with Shh (100 ng/mL) and pristimerin for 24 h. After staining with 4% paraformaldehyde, the HBVPs were labeled with Gli1 antibody and <t>Alexa</t> <t>Fluor</t> 488 Donkey anti-Rabbit <t>IgG.</t> DAPI was used to recognize the nucleus, and the images were taken with a laser scanning confocal microscope. d Pristimerin inhibits the distribution of Gli1 in the nucleus. HBVPs were collected and lysed with a NE-PERTM Nucleus and Cytoplasmic Extraction Kit following the manufacturer’s protocol, and then applied for western blotting assay. β-actin and Lamin B were set as loading control. e Pristimerin blocks the downstream of Shh/Gli1 signaling pathway. After being treated with Shh and pristimerin for 24 h, HBVP cells were lysed using RIPA buffer and subjected to western blotting assay. β-actin was set as loading control. The data are presented as mean ± SEM, n = 3. *** P
    Alexa Fluor 488 Conjugated Goat Anti Rat Igg, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor 488 conjugated goat anti rat igg/product/Abcam
    Average 94 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    alexa fluor 488 conjugated goat anti rat igg - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Confocal laser scanning microscopy utilization to visualize cytoplasmic delivery of P6 NPs. (a) Dil-P6 NPs were incubated with A2780cis cells in the presence of Alexa-Fluor 488-labeled markers of various endocytic pathways. NPs co-localized with dextran, a fluid-phase marker known to enter cells via macropinocytosis (white arrows). (b) Alexa-Fluor 488-labeled actin fibers revealed membrane ruffling and actin rearrangement (white arrows), hallmarks of uptake by macropinocytosis.

    Journal: ACS nano

    Article Title: Glutathione-Responsive Prodrug Nanoparticles for Effective Drug Delivery and Cancer Therapy

    doi: 10.1021/acsnano.8b06400

    Figure Lengend Snippet: Confocal laser scanning microscopy utilization to visualize cytoplasmic delivery of P6 NPs. (a) Dil-P6 NPs were incubated with A2780cis cells in the presence of Alexa-Fluor 488-labeled markers of various endocytic pathways. NPs co-localized with dextran, a fluid-phase marker known to enter cells via macropinocytosis (white arrows). (b) Alexa-Fluor 488-labeled actin fibers revealed membrane ruffling and actin rearrangement (white arrows), hallmarks of uptake by macropinocytosis.

    Article Snippet: After blocking with 10% FBS for 1.5 h, slices were incubated with rat anti-mouse CD31 antibodies (Abcam) at 4 °C for 1 h, washed with PBS/0.2% Triton X-100 three times, and then incubated with Alexa-Fluor 488-conjugated secondary antibodies (Goat anti-rat IgG, Abcam) for 1 h. Thereafter, slices were washed with PBS three times, stained with Hoechst 33342, and viewed under a CLSM.

    Techniques: Confocal Laser Scanning Microscopy, Incubation, Labeling, Marker

    Pristimerin treatment decreases Shh-induced pericyte motilities of angiogenesis. a Pristimerin suppresses Shh-mediated adhesion ability of endothelial cells. The cells that pre-treated with Shh (100 ng/mL) and pristimerin (125, 250, and 500 nM) were seeded at 24-well plate. After 1-h culture, the un-attached cells were removed and the attached cells were stained with 0.1% crystal violet. b Pristimerin restrains Shh-induced pericyte migration to newly formed networks in a three-dimensional co-culture system of HUVEC and HBVP. HUVECs (red) were seeded on Matrigel-coated 96-well and cultured for 2 h to allow the formation of tube network. And HBVPs (green) pre-treated with or without pristimerin were added to the endothelial tubes. The recruitment of HBVPs to HUVEC tubes were observed and photographed at 0, 2, and 12 h. Scale bar: 20 μm. c Pristimerin suppresses the nucleus translocation of Gli1in HBVPs. HBVPs were stimulated with Shh (100 ng/mL) and pristimerin for 24 h. After staining with 4% paraformaldehyde, the HBVPs were labeled with Gli1 antibody and Alexa Fluor 488 Donkey anti-Rabbit IgG. DAPI was used to recognize the nucleus, and the images were taken with a laser scanning confocal microscope. d Pristimerin inhibits the distribution of Gli1 in the nucleus. HBVPs were collected and lysed with a NE-PERTM Nucleus and Cytoplasmic Extraction Kit following the manufacturer’s protocol, and then applied for western blotting assay. β-actin and Lamin B were set as loading control. e Pristimerin blocks the downstream of Shh/Gli1 signaling pathway. After being treated with Shh and pristimerin for 24 h, HBVP cells were lysed using RIPA buffer and subjected to western blotting assay. β-actin was set as loading control. The data are presented as mean ± SEM, n = 3. *** P

    Journal: Cell Death & Disease

    Article Title: Identification of a novel tumor angiogenesis inhibitor targeting Shh/Gli1 signaling pathway in Non-small cell lung cancer

    doi: 10.1038/s41419-020-2425-0

    Figure Lengend Snippet: Pristimerin treatment decreases Shh-induced pericyte motilities of angiogenesis. a Pristimerin suppresses Shh-mediated adhesion ability of endothelial cells. The cells that pre-treated with Shh (100 ng/mL) and pristimerin (125, 250, and 500 nM) were seeded at 24-well plate. After 1-h culture, the un-attached cells were removed and the attached cells were stained with 0.1% crystal violet. b Pristimerin restrains Shh-induced pericyte migration to newly formed networks in a three-dimensional co-culture system of HUVEC and HBVP. HUVECs (red) were seeded on Matrigel-coated 96-well and cultured for 2 h to allow the formation of tube network. And HBVPs (green) pre-treated with or without pristimerin were added to the endothelial tubes. The recruitment of HBVPs to HUVEC tubes were observed and photographed at 0, 2, and 12 h. Scale bar: 20 μm. c Pristimerin suppresses the nucleus translocation of Gli1in HBVPs. HBVPs were stimulated with Shh (100 ng/mL) and pristimerin for 24 h. After staining with 4% paraformaldehyde, the HBVPs were labeled with Gli1 antibody and Alexa Fluor 488 Donkey anti-Rabbit IgG. DAPI was used to recognize the nucleus, and the images were taken with a laser scanning confocal microscope. d Pristimerin inhibits the distribution of Gli1 in the nucleus. HBVPs were collected and lysed with a NE-PERTM Nucleus and Cytoplasmic Extraction Kit following the manufacturer’s protocol, and then applied for western blotting assay. β-actin and Lamin B were set as loading control. e Pristimerin blocks the downstream of Shh/Gli1 signaling pathway. After being treated with Shh and pristimerin for 24 h, HBVP cells were lysed using RIPA buffer and subjected to western blotting assay. β-actin was set as loading control. The data are presented as mean ± SEM, n = 3. *** P

    Article Snippet: Antibodies against α-SMA (19245S), Ki67 (9449T), Akt (4685S), p-AktThr308 (13038S), ERK (4695S), p-ERKThr202/Tyr204 (4370T), PDGFR-β (3174T), p-PDGFR-βTyr857 (3170T), β-actin (4970T), Alexa Fluor 594 Donkey anti-Goat IgG (889S), Alexa Fluor 488 Donkey anti-Rabbit IgG (4416S) and HRP-conjugated anti-rabbit IgG antibody (4412S) were purchased from Cell Signaling Technology (Danvers, MA, USA).

    Techniques: Staining, Migration, Co-Culture Assay, Cell Culture, Translocation Assay, Labeling, Microscopy, Western Blot

    Pristimerin suppresses endothelial cellular motilities required for angiogenesis through inactivating Shh/Gli1 signaling pathway. a Pristimerin suppressed nucleus translocation of Gli1 in endothelial cells. After incubating with Shh (100 ng/mL) and pristimerin (125, 250, and 500 nM) for 24 h, the HUVECs and HMEC-1 cells were stained with Gli1 antibody and Alexa Fluor 488 Donkey anti-Rabbit IgG. DAPI was used to label the nucleus. The cells were observed and photographed with a laser scanning confocal microscope. Scale bar: 20 μm. b , c Pristimerin inhibits Gli1 distribution in endothelial cells. The HUVECs and HMEC-1 cells were incubated with Shh and pristimerin for 24 h, and then lysed with a NE-PERTM Nucleus and Cytoplasmic Extraction Kit. After that, proteins were subjected to western blotting assay. β-actin and Lamin B were set as loading control. The quantitative data were presented as ratios of Gli1/β-actin and Gli1/Lamin B. The representative blot and quantitative data were shown in B C, respectively. d , e Pristimerin inactivated Shh/Gli1 and its downstream signaling pathway. The cells were treated with Shh and pristimerin for 24 h and lysed using RIPA buffer. Total proteins were subjected to western blotting assay. The representative blot and quantitative data were shown in d , e , respectively. The data are presented as mean ± SEM, n = 3. *** P

    Journal: Cell Death & Disease

    Article Title: Identification of a novel tumor angiogenesis inhibitor targeting Shh/Gli1 signaling pathway in Non-small cell lung cancer

    doi: 10.1038/s41419-020-2425-0

    Figure Lengend Snippet: Pristimerin suppresses endothelial cellular motilities required for angiogenesis through inactivating Shh/Gli1 signaling pathway. a Pristimerin suppressed nucleus translocation of Gli1 in endothelial cells. After incubating with Shh (100 ng/mL) and pristimerin (125, 250, and 500 nM) for 24 h, the HUVECs and HMEC-1 cells were stained with Gli1 antibody and Alexa Fluor 488 Donkey anti-Rabbit IgG. DAPI was used to label the nucleus. The cells were observed and photographed with a laser scanning confocal microscope. Scale bar: 20 μm. b , c Pristimerin inhibits Gli1 distribution in endothelial cells. The HUVECs and HMEC-1 cells were incubated with Shh and pristimerin for 24 h, and then lysed with a NE-PERTM Nucleus and Cytoplasmic Extraction Kit. After that, proteins were subjected to western blotting assay. β-actin and Lamin B were set as loading control. The quantitative data were presented as ratios of Gli1/β-actin and Gli1/Lamin B. The representative blot and quantitative data were shown in B C, respectively. d , e Pristimerin inactivated Shh/Gli1 and its downstream signaling pathway. The cells were treated with Shh and pristimerin for 24 h and lysed using RIPA buffer. Total proteins were subjected to western blotting assay. The representative blot and quantitative data were shown in d , e , respectively. The data are presented as mean ± SEM, n = 3. *** P

    Article Snippet: Antibodies against α-SMA (19245S), Ki67 (9449T), Akt (4685S), p-AktThr308 (13038S), ERK (4695S), p-ERKThr202/Tyr204 (4370T), PDGFR-β (3174T), p-PDGFR-βTyr857 (3170T), β-actin (4970T), Alexa Fluor 594 Donkey anti-Goat IgG (889S), Alexa Fluor 488 Donkey anti-Rabbit IgG (4416S) and HRP-conjugated anti-rabbit IgG antibody (4412S) were purchased from Cell Signaling Technology (Danvers, MA, USA).

    Techniques: Translocation Assay, Staining, Microscopy, Incubation, Western Blot