Journal: Endocrinology

Article Title: Prenatal Testosterone Treatment Leads to Changes in the Morphology of KNDy Neurons, Their Inputs, and Projections to GnRH Cells in Female Sheep

doi: 10.1210/en.2014-1609

Figure Lengend Snippet: A, Confocal images showing examples of ARC kisspeptin cells from control and prenatal T animals that were used for somal and nuclear size analyses. Cells were labeled by immunofluorescence for kisspeptin (green, right panels) to measure somal perimeter and counterstained for DAPI (purple, left panels) for assessment of nuclear perimeter. Scale bar, 10 μm. B, Confocal images showing examples of POA kisspeptin (top panels) and GnRH neurons (bottom panels) from control and prenatal T animals. Scale bars, 10 μm. C, Confocal images showing examples of AgRP (top panels) and POMC neurons (bottom panels) in the ARC from control and prenatal T animals. Scale bars, 10 μm. Kisspeptin, GnRH, and AgRP were labeled using Alexa Fluor 488 as the flourophore (green), whereas POMC was labeled with Cy5 (blue); see Materials and Methods for more details.

Article Snippet: Next, sections were washed and incubated with goat anti-mouse Alexa Fluor 488 (1:100, 30 min, green, lot 41139A; Molecular Probes).

Techniques: Labeling, Immunofluorescence