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  • 99
    Thermo Fisher alexa fluor 488
    Alexa Fluor 488, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 73382 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher alexa fluor 488 phalloidin
    Alexa Fluor 488 Phalloidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 12447 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher alexafluor 488
    A, Confocal images showing examples of ARC kisspeptin cells from control and prenatal T animals that were used for somal and nuclear size analyses. Cells were labeled by immunofluorescence for kisspeptin (green, right panels) to measure somal perimeter and counterstained for DAPI (purple, left panels) for assessment of nuclear perimeter. Scale bar, 10 μm. B, Confocal images showing examples of POA kisspeptin (top panels) and GnRH neurons (bottom panels) from control and prenatal T animals. Scale bars, 10 μm. C, Confocal images showing examples of AgRP (top panels) and POMC neurons (bottom panels) in the ARC from control and prenatal T animals. Scale bars, 10 μm. Kisspeptin, GnRH, and AgRP were labeled using <t>Alexa</t> <t>Fluor</t> 488 as the flourophore (green), whereas POMC was labeled with Cy5 (blue); see Materials and Methods for more details.
    Alexafluor 488, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 10557 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher alexa fluor 488 goat anti mouse igg
    A, Confocal images showing examples of ARC kisspeptin cells from control and prenatal T animals that were used for somal and nuclear size analyses. Cells were labeled by immunofluorescence for kisspeptin (green, right panels) to measure somal perimeter and counterstained for DAPI (purple, left panels) for assessment of nuclear perimeter. Scale bar, 10 μm. B, Confocal images showing examples of POA kisspeptin (top panels) and GnRH neurons (bottom panels) from control and prenatal T animals. Scale bars, 10 μm. C, Confocal images showing examples of AgRP (top panels) and POMC neurons (bottom panels) in the ARC from control and prenatal T animals. Scale bars, 10 μm. Kisspeptin, GnRH, and AgRP were labeled using <t>Alexa</t> <t>Fluor</t> 488 as the flourophore (green), whereas POMC was labeled with Cy5 (blue); see Materials and Methods for more details.
    Alexa Fluor 488 Goat Anti Mouse Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6971 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher alexa fluor 488 conjugated goat anti rabbit igg
    A, Confocal images showing examples of ARC kisspeptin cells from control and prenatal T animals that were used for somal and nuclear size analyses. Cells were labeled by immunofluorescence for kisspeptin (green, right panels) to measure somal perimeter and counterstained for DAPI (purple, left panels) for assessment of nuclear perimeter. Scale bar, 10 μm. B, Confocal images showing examples of POA kisspeptin (top panels) and GnRH neurons (bottom panels) from control and prenatal T animals. Scale bars, 10 μm. C, Confocal images showing examples of AgRP (top panels) and POMC neurons (bottom panels) in the ARC from control and prenatal T animals. Scale bars, 10 μm. Kisspeptin, GnRH, and AgRP were labeled using <t>Alexa</t> <t>Fluor</t> 488 as the flourophore (green), whereas POMC was labeled with Cy5 (blue); see Materials and Methods for more details.
    Alexa Fluor 488 Conjugated Goat Anti Rabbit Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5695 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher goat anti rabbit igg h l cross adsorbed secondary antibody
    A, Confocal images showing examples of ARC kisspeptin cells from control and prenatal T animals that were used for somal and nuclear size analyses. Cells were labeled by immunofluorescence for kisspeptin (green, right panels) to measure somal perimeter and counterstained for DAPI (purple, left panels) for assessment of nuclear perimeter. Scale bar, 10 μm. B, Confocal images showing examples of POA kisspeptin (top panels) and GnRH neurons (bottom panels) from control and prenatal T animals. Scale bars, 10 μm. C, Confocal images showing examples of AgRP (top panels) and POMC neurons (bottom panels) in the ARC from control and prenatal T animals. Scale bars, 10 μm. Kisspeptin, GnRH, and AgRP were labeled using <t>Alexa</t> <t>Fluor</t> 488 as the flourophore (green), whereas POMC was labeled with Cy5 (blue); see Materials and Methods for more details.
    Goat Anti Rabbit Igg H L Cross Adsorbed Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 14192 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher click it edu alexa fluor 488 imaging kit
    A, Confocal images showing examples of ARC kisspeptin cells from control and prenatal T animals that were used for somal and nuclear size analyses. Cells were labeled by immunofluorescence for kisspeptin (green, right panels) to measure somal perimeter and counterstained for DAPI (purple, left panels) for assessment of nuclear perimeter. Scale bar, 10 μm. B, Confocal images showing examples of POA kisspeptin (top panels) and GnRH neurons (bottom panels) from control and prenatal T animals. Scale bars, 10 μm. C, Confocal images showing examples of AgRP (top panels) and POMC neurons (bottom panels) in the ARC from control and prenatal T animals. Scale bars, 10 μm. Kisspeptin, GnRH, and AgRP were labeled using <t>Alexa</t> <t>Fluor</t> 488 as the flourophore (green), whereas POMC was labeled with Cy5 (blue); see Materials and Methods for more details.
    Click It Edu Alexa Fluor 488 Imaging Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3758 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher alexa fluor
    A, Confocal images showing examples of ARC kisspeptin cells from control and prenatal T animals that were used for somal and nuclear size analyses. Cells were labeled by immunofluorescence for kisspeptin (green, right panels) to measure somal perimeter and counterstained for DAPI (purple, left panels) for assessment of nuclear perimeter. Scale bar, 10 μm. B, Confocal images showing examples of POA kisspeptin (top panels) and GnRH neurons (bottom panels) from control and prenatal T animals. Scale bars, 10 μm. C, Confocal images showing examples of AgRP (top panels) and POMC neurons (bottom panels) in the ARC from control and prenatal T animals. Scale bars, 10 μm. Kisspeptin, GnRH, and AgRP were labeled using <t>Alexa</t> <t>Fluor</t> 488 as the flourophore (green), whereas POMC was labeled with Cy5 (blue); see Materials and Methods for more details.
    Alexa Fluor, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 22382 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher goat anti mouse igg h l highly cross adsorbed secondary antibody
    A, Confocal images showing examples of ARC kisspeptin cells from control and prenatal T animals that were used for somal and nuclear size analyses. Cells were labeled by immunofluorescence for kisspeptin (green, right panels) to measure somal perimeter and counterstained for DAPI (purple, left panels) for assessment of nuclear perimeter. Scale bar, 10 μm. B, Confocal images showing examples of POA kisspeptin (top panels) and GnRH neurons (bottom panels) from control and prenatal T animals. Scale bars, 10 μm. C, Confocal images showing examples of AgRP (top panels) and POMC neurons (bottom panels) in the ARC from control and prenatal T animals. Scale bars, 10 μm. Kisspeptin, GnRH, and AgRP were labeled using <t>Alexa</t> <t>Fluor</t> 488 as the flourophore (green), whereas POMC was labeled with Cy5 (blue); see Materials and Methods for more details.
    Goat Anti Mouse Igg H L Highly Cross Adsorbed Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9004 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher donkey anti rabbit igg h l highly cross adsorbed secondary antibody
    A, Confocal images showing examples of ARC kisspeptin cells from control and prenatal T animals that were used for somal and nuclear size analyses. Cells were labeled by immunofluorescence for kisspeptin (green, right panels) to measure somal perimeter and counterstained for DAPI (purple, left panels) for assessment of nuclear perimeter. Scale bar, 10 μm. B, Confocal images showing examples of POA kisspeptin (top panels) and GnRH neurons (bottom panels) from control and prenatal T animals. Scale bars, 10 μm. C, Confocal images showing examples of AgRP (top panels) and POMC neurons (bottom panels) in the ARC from control and prenatal T animals. Scale bars, 10 μm. Kisspeptin, GnRH, and AgRP were labeled using <t>Alexa</t> <t>Fluor</t> 488 as the flourophore (green), whereas POMC was labeled with Cy5 (blue); see Materials and Methods for more details.
    Donkey Anti Rabbit Igg H L Highly Cross Adsorbed Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9653 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher streptavidin alexa fluor 488 conjugate
    A, Confocal images showing examples of ARC kisspeptin cells from control and prenatal T animals that were used for somal and nuclear size analyses. Cells were labeled by immunofluorescence for kisspeptin (green, right panels) to measure somal perimeter and counterstained for DAPI (purple, left panels) for assessment of nuclear perimeter. Scale bar, 10 μm. B, Confocal images showing examples of POA kisspeptin (top panels) and GnRH neurons (bottom panels) from control and prenatal T animals. Scale bars, 10 μm. C, Confocal images showing examples of AgRP (top panels) and POMC neurons (bottom panels) in the ARC from control and prenatal T animals. Scale bars, 10 μm. Kisspeptin, GnRH, and AgRP were labeled using <t>Alexa</t> <t>Fluor</t> 488 as the flourophore (green), whereas POMC was labeled with Cy5 (blue); see Materials and Methods for more details.
    Streptavidin Alexa Fluor 488 Conjugate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1870 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher alexa fluor 488 annexin v dead cell apoptosis kit
    A, Confocal images showing examples of ARC kisspeptin cells from control and prenatal T animals that were used for somal and nuclear size analyses. Cells were labeled by immunofluorescence for kisspeptin (green, right panels) to measure somal perimeter and counterstained for DAPI (purple, left panels) for assessment of nuclear perimeter. Scale bar, 10 μm. B, Confocal images showing examples of POA kisspeptin (top panels) and GnRH neurons (bottom panels) from control and prenatal T animals. Scale bars, 10 μm. C, Confocal images showing examples of AgRP (top panels) and POMC neurons (bottom panels) in the ARC from control and prenatal T animals. Scale bars, 10 μm. Kisspeptin, GnRH, and AgRP were labeled using <t>Alexa</t> <t>Fluor</t> 488 as the flourophore (green), whereas POMC was labeled with Cy5 (blue); see Materials and Methods for more details.
    Alexa Fluor 488 Annexin V Dead Cell Apoptosis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2285 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher alexa fluor 488 conjugate
    A, Confocal images showing examples of ARC kisspeptin cells from control and prenatal T animals that were used for somal and nuclear size analyses. Cells were labeled by immunofluorescence for kisspeptin (green, right panels) to measure somal perimeter and counterstained for DAPI (purple, left panels) for assessment of nuclear perimeter. Scale bar, 10 μm. B, Confocal images showing examples of POA kisspeptin (top panels) and GnRH neurons (bottom panels) from control and prenatal T animals. Scale bars, 10 μm. C, Confocal images showing examples of AgRP (top panels) and POMC neurons (bottom panels) in the ARC from control and prenatal T animals. Scale bars, 10 μm. Kisspeptin, GnRH, and AgRP were labeled using <t>Alexa</t> <t>Fluor</t> 488 as the flourophore (green), whereas POMC was labeled with Cy5 (blue); see Materials and Methods for more details.
    Alexa Fluor 488 Conjugate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1976 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    A, Confocal images showing examples of ARC kisspeptin cells from control and prenatal T animals that were used for somal and nuclear size analyses. Cells were labeled by immunofluorescence for kisspeptin (green, right panels) to measure somal perimeter and counterstained for DAPI (purple, left panels) for assessment of nuclear perimeter. Scale bar, 10 μm. B, Confocal images showing examples of POA kisspeptin (top panels) and GnRH neurons (bottom panels) from control and prenatal T animals. Scale bars, 10 μm. C, Confocal images showing examples of AgRP (top panels) and POMC neurons (bottom panels) in the ARC from control and prenatal T animals. Scale bars, 10 μm. Kisspeptin, GnRH, and AgRP were labeled using Alexa Fluor 488 as the flourophore (green), whereas POMC was labeled with Cy5 (blue); see Materials and Methods for more details.

    Journal: Endocrinology

    Article Title: Prenatal Testosterone Treatment Leads to Changes in the Morphology of KNDy Neurons, Their Inputs, and Projections to GnRH Cells in Female Sheep

    doi: 10.1210/en.2014-1609

    Figure Lengend Snippet: A, Confocal images showing examples of ARC kisspeptin cells from control and prenatal T animals that were used for somal and nuclear size analyses. Cells were labeled by immunofluorescence for kisspeptin (green, right panels) to measure somal perimeter and counterstained for DAPI (purple, left panels) for assessment of nuclear perimeter. Scale bar, 10 μm. B, Confocal images showing examples of POA kisspeptin (top panels) and GnRH neurons (bottom panels) from control and prenatal T animals. Scale bars, 10 μm. C, Confocal images showing examples of AgRP (top panels) and POMC neurons (bottom panels) in the ARC from control and prenatal T animals. Scale bars, 10 μm. Kisspeptin, GnRH, and AgRP were labeled using Alexa Fluor 488 as the flourophore (green), whereas POMC was labeled with Cy5 (blue); see Materials and Methods for more details.

    Article Snippet: Next, sections were washed and incubated with goat anti-mouse Alexa Fluor 488 (1:100, 30 min, green, lot 41139A; Molecular Probes).

    Techniques: Labeling, Immunofluorescence