alanine aminotransferase alt Search Results


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  • 99
    Millipore alanine aminotransferase alt
    Alanine Aminotransferase Alt, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 226 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Hitachi Ltd alanine transaminase alt
    Levels of aspartate aminotransferase, alanine transaminase and lactate dehydrogenase during 6 h of preservation with histidine-tryptophan-ketoglutarate solution perfusates (static cold storage vs hypothermic machine perfusion: Aspartate aminotransferase 6, 307.50 ± 52.95 vs 185.20 ± 20.46, P = 0.041). a Statistically significant difference. AST6 means the <t>AST</t> level at 6 h after liver preservation. AST: Aspartate aminotransferase; <t>ALT:</t> Alanine transaminase; LDH: Lactate dehydrogenase; SCS: Static cold storage; HMP: Hypothermic machine perfusion.
    Alanine Transaminase Alt, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 92/100, based on 446 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cayman Chemical colorimetric assay kit
    Levels of aspartate aminotransferase, alanine transaminase and lactate dehydrogenase during 6 h of preservation with histidine-tryptophan-ketoglutarate solution perfusates (static cold storage vs hypothermic machine perfusion: Aspartate aminotransferase 6, 307.50 ± 52.95 vs 185.20 ± 20.46, P = 0.041). a Statistically significant difference. AST6 means the <t>AST</t> level at 6 h after liver preservation. AST: Aspartate aminotransferase; <t>ALT:</t> Alanine transaminase; LDH: Lactate dehydrogenase; SCS: Static cold storage; HMP: Hypothermic machine perfusion.
    Colorimetric Assay Kit, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 99/100, based on 883 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Randox alanine aminotransferase alt
    Serum levels of the liver enzymes, <t>AST,</t> <t>ALT,</t> GGT, and SDH, in rabbits dosed orally with 10, 3 or 1 mg/kg/day of DMAU for 14 days (Panels A, B, C, respectively). Data points represent mean ± SE, n=3.
    Alanine Aminotransferase Alt, supplied by Randox, used in various techniques. Bioz Stars score: 92/100, based on 242 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    FUJIFILM alanine transaminase alt
    Effect of NO inhibitor in Cygb −/− mice after BDL. WT and KO mice were subjected to BDL-48 h together with L-NG-nitroarginine methyl ester (L-NAME) treatment. Control mice received drinking water (DW). ( A ) Concentration of nitrite + nitrate and cGMP in serum. ( B ) Representative macroscopic images and microscopic liver sections stained with H E. ( C ) Serum levels of <t>AST,</t> <t>ALT,</t> total bilirubin, and total bile acid (TBA). ( D ) Immunofluorescence, immunoblots and hepatic mRNA level of CD10. GAPDH was used as loading control. All gels were run under the same experimental conditions. The cropped gels are used and full-length gels are presented in Supplementary Fig. S11 . ( E ) Hepatic mRNA level of Bsep, Mdr2, Oatp1, Ntcp. ( F ) Immunohistochemistry of neutrophils and CD68 and its quantitative analyses (right insets). Open bars, WT; close bars, KO. Data represent the mean ± SD. n = 5. *p
    Alanine Transaminase Alt, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 92/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Pointe Scientific alanine aminotransferase alt
    Protective effect of murine B-1a cell secreted IL-10 during sepsis (A) Sorting of PerC B-1a cells from the WT and IL-10 -/- mice by staining them with FITC-B220, PE-CD23, PerCP-Cy5.5-CD5 and APC-CD19 Abs. (B-E) At the time of CLP, mice were injected with PBS or B-1a cells (5 × 10 5 cells) isolated from either WT or IL-10 -/- mice in 150 μL of sterile PBS. After 20 h of CLP, blood was drawn and the plasma was assessed for <t>ALT,</t> <t>AST,</t> LDH, and IL-6. Data are expressed as means ± SE (n=5 mice/group) and compared by one-way ANOVA and SNK method obtained from three independent experiments (*p
    Alanine Aminotransferase Alt, supplied by Pointe Scientific, used in various techniques. Bioz Stars score: 92/100, based on 122 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore glutamic pyruvic transaminase
    Protective effect of murine B-1a cell secreted IL-10 during sepsis (A) Sorting of PerC B-1a cells from the WT and IL-10 -/- mice by staining them with FITC-B220, PE-CD23, PerCP-Cy5.5-CD5 and APC-CD19 Abs. (B-E) At the time of CLP, mice were injected with PBS or B-1a cells (5 × 10 5 cells) isolated from either WT or IL-10 -/- mice in 150 μL of sterile PBS. After 20 h of CLP, blood was drawn and the plasma was assessed for <t>ALT,</t> <t>AST,</t> LDH, and IL-6. Data are expressed as means ± SE (n=5 mice/group) and compared by one-way ANOVA and SNK method obtained from three independent experiments (*p
    Glutamic Pyruvic Transaminase, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stanbio alanine aminotransferase alt
    Deacetylation of MTPα by SIRT4 contributes to SIRT4-mediated hepatic steatosis in HF/HS diet-fed mice. (A and B) Male C57BL/6J mice fed an HF/HS diet for 2 months were injected once a week with adenovirus encoding SIRT4, together with or without adenovirus encoding WT MTPα or 3K mutant MTPα, through the tail vein. Measurements were performed at 15 days postinfection. The mRNA levels and protein levels of SIRT4 and MTPα in the livers were determined by RT-qPCR (A) and Western blotting (B). (C) MTPα-3K acetylation levels were compared to MTPα protein levels. (D) Plasma <t>β-hydroxybutyrate</t> levels. (E) Organ weights. (F) Representative images of H E staining and Oil Red O staining of liver sections (magnification, ×100). (G) Triglyceride and cholesterol levels in livers. (H) Levels of plasma AST and <t>ALT.</t> (I) Expression levels of inflammatory genes in livers. (J) GTT (left) and ITT (right) results. *, P
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    Teco Diagnostics alanine aminotransferase alt
    In-vivo VASP repression by siRNA dampens hepatic IR injury. A ) <t>LDH-serum</t> levels after hepatic IR in in-vivo targeted repression of VASP with siRNA (siVASP) or non-targeting siRNA (siSCR) B ) Correlating serum levels of AST and C ) <t>ALT</t> of siVASP and siSCR treated WT animals. D ) Representative TTC stained liversections of both groups. E ) Histological images of platelet-neutrophil complexes (neutrophil = blue; platelet = black) in tissue sections of ischemic tissue of siVASP and siSCR treated animals (Data are shown as Mean ± SEM, n = 6, * P
    Alanine Aminotransferase Alt, supplied by Teco Diagnostics, used in various techniques. Bioz Stars score: 92/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioSino alanine aminotransferase alt
    Serum aminotransferase and arteriosclerosis levels. ( a ) <t>AST</t> and <t>ALT</t> index in serum; ( b ) AI index in serum. The data are presented as the means ± SD ( n = 10). (* ,# ) Significant difference at p
    Alanine Aminotransferase Alt, supplied by BioSino, used in various techniques. Bioz Stars score: 92/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Catachem alanine aminotransferase alt
    GL, rather than GA, prevents APAP-induced hepatocyte damage both in APAP-overdosed mice and APAP-treated LO2 cells. (A, B) GA exposure in serum (A) and in liver (B) in GA/APAP-treated mice and GL/APAP-treated mice. (C, D) Serum <t>ALT</t> and <t>AST</t> levels. (E) Cell viability of GL/APAP 20 mM-treated LO2 cells. (F) Cell viability of GA/APAP 20 mM-treated LO2 cells. (G) Cell viability of 18 β -GA/APAP 20 mM-treated LO2 cells. Data are expressed as mean ± S.E.M., n = 5–6 for both animal experiments and LO2 cell experiments. Control, saline-treated mice; GA, only GA 50 mg/kg-treated mice; GL, only GL 50 mg/kg-treated mice; APAP, saline/APAP-treated mice; GA + APAP, GA 50 mg/kg/APAP-treated mice; GL + APAP, GL 50 mg/kg/APAP-treated mice; ## P
    Alanine Aminotransferase Alt, supplied by Catachem, used in various techniques. Bioz Stars score: 92/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam alanine aminotransferase alt
    GL, rather than GA, prevents APAP-induced hepatocyte damage both in APAP-overdosed mice and APAP-treated LO2 cells. (A, B) GA exposure in serum (A) and in liver (B) in GA/APAP-treated mice and GL/APAP-treated mice. (C, D) Serum <t>ALT</t> and <t>AST</t> levels. (E) Cell viability of GL/APAP 20 mM-treated LO2 cells. (F) Cell viability of GA/APAP 20 mM-treated LO2 cells. (G) Cell viability of 18 β -GA/APAP 20 mM-treated LO2 cells. Data are expressed as mean ± S.E.M., n = 5–6 for both animal experiments and LO2 cell experiments. Control, saline-treated mice; GA, only GA 50 mg/kg-treated mice; GL, only GL 50 mg/kg-treated mice; APAP, saline/APAP-treated mice; GA + APAP, GA 50 mg/kg/APAP-treated mice; GL + APAP, GL 50 mg/kg/APAP-treated mice; ## P
    Alanine Aminotransferase Alt, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore serum alanine aminotransferase alt
    GL, rather than GA, prevents APAP-induced hepatocyte damage both in APAP-overdosed mice and APAP-treated LO2 cells. (A, B) GA exposure in serum (A) and in liver (B) in GA/APAP-treated mice and GL/APAP-treated mice. (C, D) Serum <t>ALT</t> and <t>AST</t> levels. (E) Cell viability of GL/APAP 20 mM-treated LO2 cells. (F) Cell viability of GA/APAP 20 mM-treated LO2 cells. (G) Cell viability of 18 β -GA/APAP 20 mM-treated LO2 cells. Data are expressed as mean ± S.E.M., n = 5–6 for both animal experiments and LO2 cell experiments. Control, saline-treated mice; GA, only GA 50 mg/kg-treated mice; GL, only GL 50 mg/kg-treated mice; APAP, saline/APAP-treated mice; GA + APAP, GA 50 mg/kg/APAP-treated mice; GL + APAP, GL 50 mg/kg/APAP-treated mice; ## P
    Serum Alanine Aminotransferase Alt, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    International Federation of Clinical Chemistry and Laboratory Medicine alanine aminotransferase alt
    The correlation of values and hepatic necroinflammatory activity with biopsy. The correlations of values ( a <t>ALT,</t> b <t>GGT,</t> c PT, d ALB, e H value) and hepatic necroinflammatory activity with biopsy are shown in box plots. The top and bottom of each box represents the 25th and 75th centile intervals. The line through the box in the median
    Alanine Aminotransferase Alt, supplied by International Federation of Clinical Chemistry and Laboratory Medicine, used in various techniques. Bioz Stars score: 92/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Siemens AG alanine transaminase alt
    The additional contribution of the <t>AST-to-ALT</t> ratio to predicting the risk of metabolic syndrome.
    Alanine Transaminase Alt, supplied by Siemens AG, used in various techniques. Bioz Stars score: 92/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Johnson & Johnson alanine aminotransferase alt
    Transplantation of OSKP-iPSC-Heps alleviated steatohepatitis in 18-month-old male MCD diet-fed mouse ( A ) Time course of the changes in serum <t>ALT</t> levels among control diet-fed mice, MCDD-fed mice, and MCDD-fed mice with intrasplenic transplantation of OSKP-iPSC-Heps or SnHeps. ( B ) H E staining showing the fat accumulation and severe hepatocyte vacuolization over the liver from MCDD-fed mice. Effect of intrasplenic transplantation of OSKP-iPSC-Heps or SnHeps on serum ( C ) ALT and ( D ) <t>AST</t> levels, and ( E ) hepatic triglyceride accumulation in MCDD-fed mice. In Panels C, D and E, * P
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    BioClin Therapeutics alanine aminotransferase alt
    Transplantation of OSKP-iPSC-Heps alleviated steatohepatitis in 18-month-old male MCD diet-fed mouse ( A ) Time course of the changes in serum <t>ALT</t> levels among control diet-fed mice, MCDD-fed mice, and MCDD-fed mice with intrasplenic transplantation of OSKP-iPSC-Heps or SnHeps. ( B ) H E staining showing the fat accumulation and severe hepatocyte vacuolization over the liver from MCDD-fed mice. Effect of intrasplenic transplantation of OSKP-iPSC-Heps or SnHeps on serum ( C ) ALT and ( D ) <t>AST</t> levels, and ( E ) hepatic triglyceride accumulation in MCDD-fed mice. In Panels C, D and E, * P
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    Pointe Scientific alt sgpt reagent
    Transplantation of OSKP-iPSC-Heps alleviated steatohepatitis in 18-month-old male MCD diet-fed mouse ( A ) Time course of the changes in serum <t>ALT</t> levels among control diet-fed mice, MCDD-fed mice, and MCDD-fed mice with intrasplenic transplantation of OSKP-iPSC-Heps or SnHeps. ( B ) H E staining showing the fat accumulation and severe hepatocyte vacuolization over the liver from MCDD-fed mice. Effect of intrasplenic transplantation of OSKP-iPSC-Heps or SnHeps on serum ( C ) ALT and ( D ) <t>AST</t> levels, and ( E ) hepatic triglyceride accumulation in MCDD-fed mice. In Panels C, D and E, * P
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    Alanine Transaminase ALT also known as alanine aminotransferase ALAT or serum glutamic pyruvic transaminase sGPT is a homodimeric cytoplasmic pyridoxal phosphate dependent enzyme involved in cellular nitrogen metabolism amino acid
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    Anti ALANINE TRANSAMINASE ALT SHEEP Antibody 200 601 206S
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    Sheep polyclonal Pig Alanine Transaminase antibody
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    Alanine Transaminase ALT Antibody is a Mouse Monoclonal against Alanine Transaminase ALT
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    Image Search Results


    Levels of aspartate aminotransferase, alanine transaminase and lactate dehydrogenase during 6 h of preservation with histidine-tryptophan-ketoglutarate solution perfusates (static cold storage vs hypothermic machine perfusion: Aspartate aminotransferase 6, 307.50 ± 52.95 vs 185.20 ± 20.46, P = 0.041). a Statistically significant difference. AST6 means the AST level at 6 h after liver preservation. AST: Aspartate aminotransferase; ALT: Alanine transaminase; LDH: Lactate dehydrogenase; SCS: Static cold storage; HMP: Hypothermic machine perfusion.

    Journal: World Journal of Gastroenterology : WJG

    Article Title: Influence of perfusate on liver viability during hypothermic machine perfusion

    doi: 10.3748/wjg.v21.i29.8848

    Figure Lengend Snippet: Levels of aspartate aminotransferase, alanine transaminase and lactate dehydrogenase during 6 h of preservation with histidine-tryptophan-ketoglutarate solution perfusates (static cold storage vs hypothermic machine perfusion: Aspartate aminotransferase 6, 307.50 ± 52.95 vs 185.20 ± 20.46, P = 0.041). a Statistically significant difference. AST6 means the AST level at 6 h after liver preservation. AST: Aspartate aminotransferase; ALT: Alanine transaminase; LDH: Lactate dehydrogenase; SCS: Static cold storage; HMP: Hypothermic machine perfusion.

    Article Snippet: The levels of aspartate aminotransferase (AST), alanine transaminase (ALT) and lactate dehydrogenase (LDH) were analyzed with a Hitachi 7600 automatic analyzer (Hitachi, Tokyo, Japan).

    Techniques: Preserving, AST Assay

    Levels of aspartate aminotransferase, alanine transaminase and lactate dehydrogenase during 6 h of preservation with saline perfusates (static cold storage vs hypothermic machine perfusion: Alanine transaminase 3, 225.00 ± 105.62 vs 49.50 ± 18.50, P = 0.047; LDH3, 1362.17 ± 563.30 vs 325.75 ± 147.43, P = 0.041). a Statistically significant difference. ALT3 means the ALT level at 3 h after liver preservation. AST: Aspartate aminotransferase; ALT: Alanine transaminase; LDH: Lactate dehydrogenase; SCS: Static cold storage; HMP: Hypothermic machine perfusion.

    Journal: World Journal of Gastroenterology : WJG

    Article Title: Influence of perfusate on liver viability during hypothermic machine perfusion

    doi: 10.3748/wjg.v21.i29.8848

    Figure Lengend Snippet: Levels of aspartate aminotransferase, alanine transaminase and lactate dehydrogenase during 6 h of preservation with saline perfusates (static cold storage vs hypothermic machine perfusion: Alanine transaminase 3, 225.00 ± 105.62 vs 49.50 ± 18.50, P = 0.047; LDH3, 1362.17 ± 563.30 vs 325.75 ± 147.43, P = 0.041). a Statistically significant difference. ALT3 means the ALT level at 3 h after liver preservation. AST: Aspartate aminotransferase; ALT: Alanine transaminase; LDH: Lactate dehydrogenase; SCS: Static cold storage; HMP: Hypothermic machine perfusion.

    Article Snippet: The levels of aspartate aminotransferase (AST), alanine transaminase (ALT) and lactate dehydrogenase (LDH) were analyzed with a Hitachi 7600 automatic analyzer (Hitachi, Tokyo, Japan).

    Techniques: Preserving, AST Assay

    Levels of aspartate aminotransferase, alanine transaminase and lactate dehydrogenase during 6 h of preservation with University of Wisconsin cold-storage solution perfusates (static cold storage vs hypothermic machine perfusion: Lactate dehydrogenase 6, 2880.14 ± 948.46 vs 2135.00 ± 174.27, P = 0.049). a Statistically significant difference. LDH6 means the LDH level at 6 h after liver preservation. AST: Aspartate aminotransferase; ALT: Alanine transaminase; LDH: Lactate dehydrogenase; SCS: Static cold storage; HMP: Hypothermic machine perfusion.

    Journal: World Journal of Gastroenterology : WJG

    Article Title: Influence of perfusate on liver viability during hypothermic machine perfusion

    doi: 10.3748/wjg.v21.i29.8848

    Figure Lengend Snippet: Levels of aspartate aminotransferase, alanine transaminase and lactate dehydrogenase during 6 h of preservation with University of Wisconsin cold-storage solution perfusates (static cold storage vs hypothermic machine perfusion: Lactate dehydrogenase 6, 2880.14 ± 948.46 vs 2135.00 ± 174.27, P = 0.049). a Statistically significant difference. LDH6 means the LDH level at 6 h after liver preservation. AST: Aspartate aminotransferase; ALT: Alanine transaminase; LDH: Lactate dehydrogenase; SCS: Static cold storage; HMP: Hypothermic machine perfusion.

    Article Snippet: The levels of aspartate aminotransferase (AST), alanine transaminase (ALT) and lactate dehydrogenase (LDH) were analyzed with a Hitachi 7600 automatic analyzer (Hitachi, Tokyo, Japan).

    Techniques: Preserving, AST Assay

    Propofol post‐conditioning attenuated liver injury in donor liver. ( A ) Liver tissues were stained with H E, the pathology was observed under microscope (×400). ( B ) Immunofluorescence staining was performed with Nrf2 antibody, nuclei were stained with DAPI . ( C ) Suzike's injury score for liver damage degree. ( D ) Nucleus‐positive cells percentage of Nrf2. ( E – H ) Levels of aspartate aminotransferase ( AST ), alanine aminotransferase ( ALT ), prothrombin time ( PT ) and total bilirubin ( TBIL ) were measured preoperatively (base), at the end of the surgery (0 hrs), 6 hrs(6 hrs), 24 hrs(24 hrs), 2 days(2 days), 3 days(3 days), 5 days(5 days), 7 days(7 days) and 14 days(14 days) after surgery. The abnormally distribution data were expressed as median * P

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Propofol post‐conditioning alleviates hepatic ischaemia reperfusion injury via BRG1‐mediated Nrf2/ HO‐1 transcriptional activation in human and mice

    doi: 10.1111/jcmm.13279

    Figure Lengend Snippet: Propofol post‐conditioning attenuated liver injury in donor liver. ( A ) Liver tissues were stained with H E, the pathology was observed under microscope (×400). ( B ) Immunofluorescence staining was performed with Nrf2 antibody, nuclei were stained with DAPI . ( C ) Suzike's injury score for liver damage degree. ( D ) Nucleus‐positive cells percentage of Nrf2. ( E – H ) Levels of aspartate aminotransferase ( AST ), alanine aminotransferase ( ALT ), prothrombin time ( PT ) and total bilirubin ( TBIL ) were measured preoperatively (base), at the end of the surgery (0 hrs), 6 hrs(6 hrs), 24 hrs(24 hrs), 2 days(2 days), 3 days(3 days), 5 days(5 days), 7 days(7 days) and 14 days(14 days) after surgery. The abnormally distribution data were expressed as median * P

    Article Snippet: Liver function assessment Blood samples from patients and animals were centrifuged (1500 g, 15 min., 4°C), and serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were determined by a 7180 Biochemical Analyzer (Hitachi, Japan).

    Techniques: Staining, Microscopy, Immunofluorescence, AST Assay

    Propofol attenuated liver IR injury and up‐regulated the expression of BRG 1, Nrf2, HO ‐1 and NQO 1 in mice. ( A ) Liver tissues of mice that were subjected to hepatic ischaemia reperfusion and treated with propofol were stained with H E and observed under microscope (×100). ( B – D ) The levels of AST , ALT and 8‐isoprostane were measured. ( E – H ) The expressions of Brg1, Nrf2, HO ‐1 and NQO 1 were assessed by Western blot. * P

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Propofol post‐conditioning alleviates hepatic ischaemia reperfusion injury via BRG1‐mediated Nrf2/ HO‐1 transcriptional activation in human and mice

    doi: 10.1111/jcmm.13279

    Figure Lengend Snippet: Propofol attenuated liver IR injury and up‐regulated the expression of BRG 1, Nrf2, HO ‐1 and NQO 1 in mice. ( A ) Liver tissues of mice that were subjected to hepatic ischaemia reperfusion and treated with propofol were stained with H E and observed under microscope (×100). ( B – D ) The levels of AST , ALT and 8‐isoprostane were measured. ( E – H ) The expressions of Brg1, Nrf2, HO ‐1 and NQO 1 were assessed by Western blot. * P

    Article Snippet: Liver function assessment Blood samples from patients and animals were centrifuged (1500 g, 15 min., 4°C), and serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were determined by a 7180 Biochemical Analyzer (Hitachi, Japan).

    Techniques: Expressing, Mouse Assay, Staining, Microscopy, AST Assay, Western Blot

    Serum levels of the liver enzymes, AST, ALT, GGT, and SDH, in rabbits dosed orally with 10, 3 or 1 mg/kg/day of DMAU for 14 days (Panels A, B, C, respectively). Data points represent mean ± SE, n=3.

    Journal: Journal of andrology

    Article Title: Effects of Synthetic Androgens on Liver Function Using the Rabbit as a Model *Effects of Synthetic Androgens on Liver Function Using the Rabbit as a Model * †

    doi: 10.2164/jandrol.109.009365

    Figure Lengend Snippet: Serum levels of the liver enzymes, AST, ALT, GGT, and SDH, in rabbits dosed orally with 10, 3 or 1 mg/kg/day of DMAU for 14 days (Panels A, B, C, respectively). Data points represent mean ± SE, n=3.

    Article Snippet: Reagents for alanine aminotransferase (ALT), aspartate aminotransferase (AST), and gamma glutamyl transpeptidase (GGT) assays were purchased from Randox Laboratories, Ltd. (Oceanside CA), and reagents for sorbitol dehydrogenase (SDH) assays were purchased from Sigma-Aldrich, Inc.

    Techniques: AST Assay

    Serum levels of the liver enzymes, AST, ALT, GGT, and SDH, in rabbits dosed orally with 10, 3 or 1 mg/kg/day of MENT for 14 days (Panels A, B, C, respectively). Data points represent mean ± SE, n=3.

    Journal: Journal of andrology

    Article Title: Effects of Synthetic Androgens on Liver Function Using the Rabbit as a Model *Effects of Synthetic Androgens on Liver Function Using the Rabbit as a Model * †

    doi: 10.2164/jandrol.109.009365

    Figure Lengend Snippet: Serum levels of the liver enzymes, AST, ALT, GGT, and SDH, in rabbits dosed orally with 10, 3 or 1 mg/kg/day of MENT for 14 days (Panels A, B, C, respectively). Data points represent mean ± SE, n=3.

    Article Snippet: Reagents for alanine aminotransferase (ALT), aspartate aminotransferase (AST), and gamma glutamyl transpeptidase (GGT) assays were purchased from Randox Laboratories, Ltd. (Oceanside CA), and reagents for sorbitol dehydrogenase (SDH) assays were purchased from Sigma-Aldrich, Inc.

    Techniques: AST Assay

    Serum levels of the liver enzymes, AST, ALT, GGT, and SDH in rabbits dosed orally with 10 mg/kg/day of T (A) or MT (B) for 14 days. Data points represent mean ± SE, n=3.

    Journal: Journal of andrology

    Article Title: Effects of Synthetic Androgens on Liver Function Using the Rabbit as a Model *Effects of Synthetic Androgens on Liver Function Using the Rabbit as a Model * †

    doi: 10.2164/jandrol.109.009365

    Figure Lengend Snippet: Serum levels of the liver enzymes, AST, ALT, GGT, and SDH in rabbits dosed orally with 10 mg/kg/day of T (A) or MT (B) for 14 days. Data points represent mean ± SE, n=3.

    Article Snippet: Reagents for alanine aminotransferase (ALT), aspartate aminotransferase (AST), and gamma glutamyl transpeptidase (GGT) assays were purchased from Randox Laboratories, Ltd. (Oceanside CA), and reagents for sorbitol dehydrogenase (SDH) assays were purchased from Sigma-Aldrich, Inc.

    Techniques: AST Assay

    Effect of NO inhibitor in Cygb −/− mice after BDL. WT and KO mice were subjected to BDL-48 h together with L-NG-nitroarginine methyl ester (L-NAME) treatment. Control mice received drinking water (DW). ( A ) Concentration of nitrite + nitrate and cGMP in serum. ( B ) Representative macroscopic images and microscopic liver sections stained with H E. ( C ) Serum levels of AST, ALT, total bilirubin, and total bile acid (TBA). ( D ) Immunofluorescence, immunoblots and hepatic mRNA level of CD10. GAPDH was used as loading control. All gels were run under the same experimental conditions. The cropped gels are used and full-length gels are presented in Supplementary Fig. S11 . ( E ) Hepatic mRNA level of Bsep, Mdr2, Oatp1, Ntcp. ( F ) Immunohistochemistry of neutrophils and CD68 and its quantitative analyses (right insets). Open bars, WT; close bars, KO. Data represent the mean ± SD. n = 5. *p

    Journal: Scientific Reports

    Article Title: Possible Involvement of Nitric Oxide in Enhanced Liver Injury and Fibrogenesis during Cholestasis in Cytoglobin-deficient Mice

    doi: 10.1038/srep41888

    Figure Lengend Snippet: Effect of NO inhibitor in Cygb −/− mice after BDL. WT and KO mice were subjected to BDL-48 h together with L-NG-nitroarginine methyl ester (L-NAME) treatment. Control mice received drinking water (DW). ( A ) Concentration of nitrite + nitrate and cGMP in serum. ( B ) Representative macroscopic images and microscopic liver sections stained with H E. ( C ) Serum levels of AST, ALT, total bilirubin, and total bile acid (TBA). ( D ) Immunofluorescence, immunoblots and hepatic mRNA level of CD10. GAPDH was used as loading control. All gels were run under the same experimental conditions. The cropped gels are used and full-length gels are presented in Supplementary Fig. S11 . ( E ) Hepatic mRNA level of Bsep, Mdr2, Oatp1, Ntcp. ( F ) Immunohistochemistry of neutrophils and CD68 and its quantitative analyses (right insets). Open bars, WT; close bars, KO. Data represent the mean ± SD. n = 5. *p

    Article Snippet: Measurement of AST and ALT and Total Bile Acid Assay Aspartate transaminase (AST), alanine transaminase (ALT) and total bile acid (TBA) were measured in serum using a commercially available kit (Wako, Osaka, Japan) according to the manufacturer’s protocol.

    Techniques: Mouse Assay, Concentration Assay, Staining, AST Assay, Immunofluorescence, Western Blot, Immunohistochemistry

    Effect of NO donor on BDL-induced liver injury in WT and Cygb −/− mice after BDL. WT and KO were subjected to BDL-48 h together with saline or sodium nitroprusside (SNP) treatment. ( A ) Concentration of nitrite + nitrate and cGMP in serum. ( B ) Representative macroscopic images, microscopic liver sections stained with H E. ( C ) Serum AST, ALT, total bilirubin, and total bile acid (TBA). ( D ) Immunofluorescent staining, immunoblots and hepatic mRNA level of CD10. GAPDH was used as loading control. All gels were run under the same experimental conditions. The cropped gels are used and full-length gels are presented in Supplementary Fig. S12 . ( E ) Hepatic mRNA level of Bsep, Mdr2, Oatp1, Ntcp. ( F ) Immunohistochemistry of neutrophils and CD68 and its quantitative analyses (right insets). Open bars, WT; close bars, KO. Data represent the mean ± SD. n = 5. *p

    Journal: Scientific Reports

    Article Title: Possible Involvement of Nitric Oxide in Enhanced Liver Injury and Fibrogenesis during Cholestasis in Cytoglobin-deficient Mice

    doi: 10.1038/srep41888

    Figure Lengend Snippet: Effect of NO donor on BDL-induced liver injury in WT and Cygb −/− mice after BDL. WT and KO were subjected to BDL-48 h together with saline or sodium nitroprusside (SNP) treatment. ( A ) Concentration of nitrite + nitrate and cGMP in serum. ( B ) Representative macroscopic images, microscopic liver sections stained with H E. ( C ) Serum AST, ALT, total bilirubin, and total bile acid (TBA). ( D ) Immunofluorescent staining, immunoblots and hepatic mRNA level of CD10. GAPDH was used as loading control. All gels were run under the same experimental conditions. The cropped gels are used and full-length gels are presented in Supplementary Fig. S12 . ( E ) Hepatic mRNA level of Bsep, Mdr2, Oatp1, Ntcp. ( F ) Immunohistochemistry of neutrophils and CD68 and its quantitative analyses (right insets). Open bars, WT; close bars, KO. Data represent the mean ± SD. n = 5. *p

    Article Snippet: Measurement of AST and ALT and Total Bile Acid Assay Aspartate transaminase (AST), alanine transaminase (ALT) and total bile acid (TBA) were measured in serum using a commercially available kit (Wako, Osaka, Japan) according to the manufacturer’s protocol.

    Techniques: Mouse Assay, Concentration Assay, Staining, AST Assay, Western Blot, Immunohistochemistry

    Severe liver injury in Cygb −/− mice under BDL. ( A ) Kaplan-Meier curve, n = 19 per group. Representative macroscopic images ( B ) and microscopic liver sections stained with H E ( C ) in sham, acute BDL (24–72 h) and chronic BDL (1–3 W). Original magnification, x40. Yellow and black arrows indicate bile infarcts. ( D ) Quantification of area of bile infarcts. ( E ) Levels of serum AST, ALT, and total bilirubin, and hepatic total bile acid (TBA). Data represent the mean ± SD. Sham (n = 3), BDL (n = 4–8). *p

    Journal: Scientific Reports

    Article Title: Possible Involvement of Nitric Oxide in Enhanced Liver Injury and Fibrogenesis during Cholestasis in Cytoglobin-deficient Mice

    doi: 10.1038/srep41888

    Figure Lengend Snippet: Severe liver injury in Cygb −/− mice under BDL. ( A ) Kaplan-Meier curve, n = 19 per group. Representative macroscopic images ( B ) and microscopic liver sections stained with H E ( C ) in sham, acute BDL (24–72 h) and chronic BDL (1–3 W). Original magnification, x40. Yellow and black arrows indicate bile infarcts. ( D ) Quantification of area of bile infarcts. ( E ) Levels of serum AST, ALT, and total bilirubin, and hepatic total bile acid (TBA). Data represent the mean ± SD. Sham (n = 3), BDL (n = 4–8). *p

    Article Snippet: Measurement of AST and ALT and Total Bile Acid Assay Aspartate transaminase (AST), alanine transaminase (ALT) and total bile acid (TBA) were measured in serum using a commercially available kit (Wako, Osaka, Japan) according to the manufacturer’s protocol.

    Techniques: Mouse Assay, Staining, AST Assay

    Protective effect of murine B-1a cell secreted IL-10 during sepsis (A) Sorting of PerC B-1a cells from the WT and IL-10 -/- mice by staining them with FITC-B220, PE-CD23, PerCP-Cy5.5-CD5 and APC-CD19 Abs. (B-E) At the time of CLP, mice were injected with PBS or B-1a cells (5 × 10 5 cells) isolated from either WT or IL-10 -/- mice in 150 μL of sterile PBS. After 20 h of CLP, blood was drawn and the plasma was assessed for ALT, AST, LDH, and IL-6. Data are expressed as means ± SE (n=5 mice/group) and compared by one-way ANOVA and SNK method obtained from three independent experiments (*p

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: B-1a Cells Protect Mice from Sepsis: Critical Role of cAMP-response Element Binding Protein (CREB)

    doi: 10.4049/jimmunol.1602056

    Figure Lengend Snippet: Protective effect of murine B-1a cell secreted IL-10 during sepsis (A) Sorting of PerC B-1a cells from the WT and IL-10 -/- mice by staining them with FITC-B220, PE-CD23, PerCP-Cy5.5-CD5 and APC-CD19 Abs. (B-E) At the time of CLP, mice were injected with PBS or B-1a cells (5 × 10 5 cells) isolated from either WT or IL-10 -/- mice in 150 μL of sterile PBS. After 20 h of CLP, blood was drawn and the plasma was assessed for ALT, AST, LDH, and IL-6. Data are expressed as means ± SE (n=5 mice/group) and compared by one-way ANOVA and SNK method obtained from three independent experiments (*p

    Article Snippet: Alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) levels were measured using assay kits from Pointe Scientific (Canton, MI).

    Techniques: Mouse Assay, Staining, Injection, Isolation, AST Assay

    Therapeutic potential of adoptive transfer of B-1a cells during sepsis At the time of CLP, mice were treated with either PBS as vehicle or 5 × 10 5 PerC B-1a cells in 150 μl of PBS by i.p. injection. After 20 h, blood was drawn to assess plasma levels of injury markers, (A) ALT, (B) AST, and (C) LDH and pro-inflammatory cytokines (D) IL-6, (E) IL-1β, and chemokine (F) MIP-2. Aside from blood, after 20 h of CLP PerC washouts were collected, spun down, and the supernatants were used to assess (G) IL-6 and (H) IL-1β by ELISA. Data are expressed as means ± SE (n=6-9 mice/group) and compared by one-way ANOVA and SNK method obtained from five independent experiments (*p

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: B-1a Cells Protect Mice from Sepsis: Critical Role of cAMP-response Element Binding Protein (CREB)

    doi: 10.4049/jimmunol.1602056

    Figure Lengend Snippet: Therapeutic potential of adoptive transfer of B-1a cells during sepsis At the time of CLP, mice were treated with either PBS as vehicle or 5 × 10 5 PerC B-1a cells in 150 μl of PBS by i.p. injection. After 20 h, blood was drawn to assess plasma levels of injury markers, (A) ALT, (B) AST, and (C) LDH and pro-inflammatory cytokines (D) IL-6, (E) IL-1β, and chemokine (F) MIP-2. Aside from blood, after 20 h of CLP PerC washouts were collected, spun down, and the supernatants were used to assess (G) IL-6 and (H) IL-1β by ELISA. Data are expressed as means ± SE (n=6-9 mice/group) and compared by one-way ANOVA and SNK method obtained from five independent experiments (*p

    Article Snippet: Alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) levels were measured using assay kits from Pointe Scientific (Canton, MI).

    Techniques: Adoptive Transfer Assay, Mouse Assay, Injection, AST Assay, Enzyme-linked Immunosorbent Assay

    Status of B-1a cell deficient CD19 -/- mice during sepsis After 20 h of CLP induced in WT and CD19 -/- mice, blood was collected and plasma was subjected to assay for (A) ALT, (B) AST, (C) LDH and (D) IL-6. Data are expressed as means ± SE (n=6-9 mice/group) and compared by one-way ANOVA and SNK method obtained from five independent experiments (*p

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: B-1a Cells Protect Mice from Sepsis: Critical Role of cAMP-response Element Binding Protein (CREB)

    doi: 10.4049/jimmunol.1602056

    Figure Lengend Snippet: Status of B-1a cell deficient CD19 -/- mice during sepsis After 20 h of CLP induced in WT and CD19 -/- mice, blood was collected and plasma was subjected to assay for (A) ALT, (B) AST, (C) LDH and (D) IL-6. Data are expressed as means ± SE (n=6-9 mice/group) and compared by one-way ANOVA and SNK method obtained from five independent experiments (*p

    Article Snippet: Alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) levels were measured using assay kits from Pointe Scientific (Canton, MI).

    Techniques: Mouse Assay, AST Assay

    Deacetylation of MTPα by SIRT4 contributes to SIRT4-mediated hepatic steatosis in HF/HS diet-fed mice. (A and B) Male C57BL/6J mice fed an HF/HS diet for 2 months were injected once a week with adenovirus encoding SIRT4, together with or without adenovirus encoding WT MTPα or 3K mutant MTPα, through the tail vein. Measurements were performed at 15 days postinfection. The mRNA levels and protein levels of SIRT4 and MTPα in the livers were determined by RT-qPCR (A) and Western blotting (B). (C) MTPα-3K acetylation levels were compared to MTPα protein levels. (D) Plasma β-hydroxybutyrate levels. (E) Organ weights. (F) Representative images of H E staining and Oil Red O staining of liver sections (magnification, ×100). (G) Triglyceride and cholesterol levels in livers. (H) Levels of plasma AST and ALT. (I) Expression levels of inflammatory genes in livers. (J) GTT (left) and ITT (right) results. *, P

    Journal: Molecular and Cellular Biology

    Article Title: Acetylation of Mitochondrial Trifunctional Protein α-Subunit Enhances Its Stability To Promote Fatty Acid Oxidation and Is Decreased in Nonalcoholic Fatty Liver Disease

    doi: 10.1128/MCB.00227-16

    Figure Lengend Snippet: Deacetylation of MTPα by SIRT4 contributes to SIRT4-mediated hepatic steatosis in HF/HS diet-fed mice. (A and B) Male C57BL/6J mice fed an HF/HS diet for 2 months were injected once a week with adenovirus encoding SIRT4, together with or without adenovirus encoding WT MTPα or 3K mutant MTPα, through the tail vein. Measurements were performed at 15 days postinfection. The mRNA levels and protein levels of SIRT4 and MTPα in the livers were determined by RT-qPCR (A) and Western blotting (B). (C) MTPα-3K acetylation levels were compared to MTPα protein levels. (D) Plasma β-hydroxybutyrate levels. (E) Organ weights. (F) Representative images of H E staining and Oil Red O staining of liver sections (magnification, ×100). (G) Triglyceride and cholesterol levels in livers. (H) Levels of plasma AST and ALT. (I) Expression levels of inflammatory genes in livers. (J) GTT (left) and ITT (right) results. *, P

    Article Snippet: Liver and plasma triglyceride and cholesterol levels and plasma β-hydroxybutyrate, alanine aminotransferase (ALT), and aspartate transaminase (AST) levels were measured using kits from Stanbio.

    Techniques: Mouse Assay, Injection, Mutagenesis, Quantitative RT-PCR, Western Blot, Staining, AST Assay, Expressing

    Knockdown of SIRT4 alone could phenocopy the effects of MTPα-3K mutant expression in mouse livers. (A and B) Male C57BL/6J mice fed an HF/HS diet for 2 months were injected once a week with adenovirus harboring shNC or shSIRT4. Measurements were performed at 15 days postinfection. The mRNA levels and protein levels of SIRT4 and MTPα in the livers were determined by RT-qPCR (A) and Western blotting (B), respectively. (C) MTPα-3K acetylation levels were compared to MTPα protein levels. (D) Plasma β-hydroxybutyrate levels. (E) Organ weights. (F) Representative images of H E staining and Oil Red O staining of liver sections (magnification, ×100). (G) Triglyceride and cholesterol levels in livers. (H) Levels of AST and ALT in plasma. (I) Expression levels of inflammatory genes in livers. (J) GTT (left) and ITT (right) results. *, P

    Journal: Molecular and Cellular Biology

    Article Title: Acetylation of Mitochondrial Trifunctional Protein α-Subunit Enhances Its Stability To Promote Fatty Acid Oxidation and Is Decreased in Nonalcoholic Fatty Liver Disease

    doi: 10.1128/MCB.00227-16

    Figure Lengend Snippet: Knockdown of SIRT4 alone could phenocopy the effects of MTPα-3K mutant expression in mouse livers. (A and B) Male C57BL/6J mice fed an HF/HS diet for 2 months were injected once a week with adenovirus harboring shNC or shSIRT4. Measurements were performed at 15 days postinfection. The mRNA levels and protein levels of SIRT4 and MTPα in the livers were determined by RT-qPCR (A) and Western blotting (B), respectively. (C) MTPα-3K acetylation levels were compared to MTPα protein levels. (D) Plasma β-hydroxybutyrate levels. (E) Organ weights. (F) Representative images of H E staining and Oil Red O staining of liver sections (magnification, ×100). (G) Triglyceride and cholesterol levels in livers. (H) Levels of AST and ALT in plasma. (I) Expression levels of inflammatory genes in livers. (J) GTT (left) and ITT (right) results. *, P

    Article Snippet: Liver and plasma triglyceride and cholesterol levels and plasma β-hydroxybutyrate, alanine aminotransferase (ALT), and aspartate transaminase (AST) levels were measured using kits from Stanbio.

    Techniques: Mutagenesis, Expressing, Mouse Assay, Injection, Quantitative RT-PCR, Western Blot, Staining, AST Assay

    Acetylation of MTPα ameliorates hepatic steatosis in mice challenged by an HF/HS diet. (A and B) Male C57BL/6J mice fed an HF/HS diet for 2 months were injected once a week with adenovirus harboring shNC or shMTPα, together with or without adenovirus harboring shRNA-resistant WT MTPα or 3K mutant MTPα, through the tail vein. Measurements were performed at 15 days postinfection. The mRNA levels and protein levels of MTPα in the livers were determined by RT-qPCR (A) and Western blotting (B), respectively. (C) Plasma β-hydroxybutyrate levels. (D) Organ weights. (E) Representative images of H E staining and Oil Red O staining of liver sections (magnification, ×100). (F) Triglyceride and cholesterol levels in livers. (G) Levels of AST and ALT in plasma. (H) Expression levels of inflammatory genes in livers. (I) Results of GTT (left) and ITT (right). *, P

    Journal: Molecular and Cellular Biology

    Article Title: Acetylation of Mitochondrial Trifunctional Protein α-Subunit Enhances Its Stability To Promote Fatty Acid Oxidation and Is Decreased in Nonalcoholic Fatty Liver Disease

    doi: 10.1128/MCB.00227-16

    Figure Lengend Snippet: Acetylation of MTPα ameliorates hepatic steatosis in mice challenged by an HF/HS diet. (A and B) Male C57BL/6J mice fed an HF/HS diet for 2 months were injected once a week with adenovirus harboring shNC or shMTPα, together with or without adenovirus harboring shRNA-resistant WT MTPα or 3K mutant MTPα, through the tail vein. Measurements were performed at 15 days postinfection. The mRNA levels and protein levels of MTPα in the livers were determined by RT-qPCR (A) and Western blotting (B), respectively. (C) Plasma β-hydroxybutyrate levels. (D) Organ weights. (E) Representative images of H E staining and Oil Red O staining of liver sections (magnification, ×100). (F) Triglyceride and cholesterol levels in livers. (G) Levels of AST and ALT in plasma. (H) Expression levels of inflammatory genes in livers. (I) Results of GTT (left) and ITT (right). *, P

    Article Snippet: Liver and plasma triglyceride and cholesterol levels and plasma β-hydroxybutyrate, alanine aminotransferase (ALT), and aspartate transaminase (AST) levels were measured using kits from Stanbio.

    Techniques: Mouse Assay, Injection, shRNA, Mutagenesis, Quantitative RT-PCR, Western Blot, Staining, AST Assay, Expressing

    In-vivo VASP repression by siRNA dampens hepatic IR injury. A ) LDH-serum levels after hepatic IR in in-vivo targeted repression of VASP with siRNA (siVASP) or non-targeting siRNA (siSCR) B ) Correlating serum levels of AST and C ) ALT of siVASP and siSCR treated WT animals. D ) Representative TTC stained liversections of both groups. E ) Histological images of platelet-neutrophil complexes (neutrophil = blue; platelet = black) in tissue sections of ischemic tissue of siVASP and siSCR treated animals (Data are shown as Mean ± SEM, n = 6, * P

    Journal: PLoS ONE

    Article Title: Phosphorylation of Vasodilator-Stimulated Phosphoprotein (VASP) Dampens Hepatic Ischemia-Reperfusion Injury

    doi: 10.1371/journal.pone.0029494

    Figure Lengend Snippet: In-vivo VASP repression by siRNA dampens hepatic IR injury. A ) LDH-serum levels after hepatic IR in in-vivo targeted repression of VASP with siRNA (siVASP) or non-targeting siRNA (siSCR) B ) Correlating serum levels of AST and C ) ALT of siVASP and siSCR treated WT animals. D ) Representative TTC stained liversections of both groups. E ) Histological images of platelet-neutrophil complexes (neutrophil = blue; platelet = black) in tissue sections of ischemic tissue of siVASP and siSCR treated animals (Data are shown as Mean ± SEM, n = 6, * P

    Article Snippet: Tissue damage was determined through blood serum levels of lactate dehydrogenase (LDH) (Randox, Crumlin, United Kingdom), aspartate (AST) and alanine aminotransferase (ALT) (Teco Diagnostics, Anaheim, USA).

    Techniques: In Vivo, AST Assay, Staining

    Hematopoietic VASP repression reduces hepatic IR injury. A ) LDH-serum levels in chimeric animals ( VASP −/− →WT and WT→ VASP −/− ) and control transplanted animals after 30 minutes of hepatic ischemia followed by 3 hours reperfusion. B ) Correlating serum levels of AST and C ) ALT of the chimeric and control animals D ) Representative TTC stained liversections of ischemic tissue. E ) Representative histological images of infracted hepatic areas of infiltrated platelet-neutrophil complexes (neutrophil = blue; platelets = black) of chimeric animals ( VASP −/− →WT and WT→ VASP −/− ) and control transplanted (WT→WT and VASP −/− → VASP −/− ) animals (Data are shown as Mean ± SEM, n = 6, * P

    Journal: PLoS ONE

    Article Title: Phosphorylation of Vasodilator-Stimulated Phosphoprotein (VASP) Dampens Hepatic Ischemia-Reperfusion Injury

    doi: 10.1371/journal.pone.0029494

    Figure Lengend Snippet: Hematopoietic VASP repression reduces hepatic IR injury. A ) LDH-serum levels in chimeric animals ( VASP −/− →WT and WT→ VASP −/− ) and control transplanted animals after 30 minutes of hepatic ischemia followed by 3 hours reperfusion. B ) Correlating serum levels of AST and C ) ALT of the chimeric and control animals D ) Representative TTC stained liversections of ischemic tissue. E ) Representative histological images of infracted hepatic areas of infiltrated platelet-neutrophil complexes (neutrophil = blue; platelets = black) of chimeric animals ( VASP −/− →WT and WT→ VASP −/− ) and control transplanted (WT→WT and VASP −/− → VASP −/− ) animals (Data are shown as Mean ± SEM, n = 6, * P

    Article Snippet: Tissue damage was determined through blood serum levels of lactate dehydrogenase (LDH) (Randox, Crumlin, United Kingdom), aspartate (AST) and alanine aminotransferase (ALT) (Teco Diagnostics, Anaheim, USA).

    Techniques: AST Assay, Staining

    VASP phosphorylation on Ser 235 using ANP dampens hepatic IR injury. A ) LDH-serum levels in WT and VASP −/− animals after 30 minutes of hepatic ischemia followed by 3 hours reperfusion with infusion of saline (vehicle) or ANP during reperfusion. B ) Correlating serum levels of AST and C ) ALT of vehicle or ANP treated WT and VASP −/− animals. D ) Representative TTC stained liversections of ischemic tissue. E ) Histological images of platelet-neutrophil complexes (neutrophil = blue; platelet = black) in tissue sections of ischemic liver lobes of vehicle or ANP treated WT and VASP −/− animals (Data are shown as Mean ± SEM, n = 6, * P

    Journal: PLoS ONE

    Article Title: Phosphorylation of Vasodilator-Stimulated Phosphoprotein (VASP) Dampens Hepatic Ischemia-Reperfusion Injury

    doi: 10.1371/journal.pone.0029494

    Figure Lengend Snippet: VASP phosphorylation on Ser 235 using ANP dampens hepatic IR injury. A ) LDH-serum levels in WT and VASP −/− animals after 30 minutes of hepatic ischemia followed by 3 hours reperfusion with infusion of saline (vehicle) or ANP during reperfusion. B ) Correlating serum levels of AST and C ) ALT of vehicle or ANP treated WT and VASP −/− animals. D ) Representative TTC stained liversections of ischemic tissue. E ) Histological images of platelet-neutrophil complexes (neutrophil = blue; platelet = black) in tissue sections of ischemic liver lobes of vehicle or ANP treated WT and VASP −/− animals (Data are shown as Mean ± SEM, n = 6, * P

    Article Snippet: Tissue damage was determined through blood serum levels of lactate dehydrogenase (LDH) (Randox, Crumlin, United Kingdom), aspartate (AST) and alanine aminotransferase (ALT) (Teco Diagnostics, Anaheim, USA).

    Techniques: Aqueous Normal-phase Chromatography, AST Assay, Staining

    VASP phosphorylation on Ser 153 using PGE 1 dampens hepatic IR injury. A ) LDH-serum levels in WT and VASP −/− animals after 30 minutes of hepatic ischemia followed by 3 hours reperfusion with infusion of saline (vehicle) or PGE1 during reperfusion. B ) Correlating serum levels of AST and C ) ALT of vehicle or PGE1 treated WT and VASP −/− animals. D ) Representative TTC stained liversections of ischemic tissue. E ) Histological images of platelet-neutrophil complexes (neutrophil = blue; platelet = black) in tissue sections of ischemic liver lobes of vehicle or PGE 1 treated WT and VASP −/− animals (Data are shown as Mean ± SEM, n = 6, * P

    Journal: PLoS ONE

    Article Title: Phosphorylation of Vasodilator-Stimulated Phosphoprotein (VASP) Dampens Hepatic Ischemia-Reperfusion Injury

    doi: 10.1371/journal.pone.0029494

    Figure Lengend Snippet: VASP phosphorylation on Ser 153 using PGE 1 dampens hepatic IR injury. A ) LDH-serum levels in WT and VASP −/− animals after 30 minutes of hepatic ischemia followed by 3 hours reperfusion with infusion of saline (vehicle) or PGE1 during reperfusion. B ) Correlating serum levels of AST and C ) ALT of vehicle or PGE1 treated WT and VASP −/− animals. D ) Representative TTC stained liversections of ischemic tissue. E ) Histological images of platelet-neutrophil complexes (neutrophil = blue; platelet = black) in tissue sections of ischemic liver lobes of vehicle or PGE 1 treated WT and VASP −/− animals (Data are shown as Mean ± SEM, n = 6, * P

    Article Snippet: Tissue damage was determined through blood serum levels of lactate dehydrogenase (LDH) (Randox, Crumlin, United Kingdom), aspartate (AST) and alanine aminotransferase (ALT) (Teco Diagnostics, Anaheim, USA).

    Techniques: AST Assay, Staining

    VASP −/− animals demonstrate reduced hepatic IR injury. A ) LDH-serum levels of VASP −/− and WT mice after 30 minutes ischemia and 3 hours reperfusion in U/L. B ) Correlating serum levels of AST and C ) ALT of VASP −/− and WT animals. D ) Representative TTC stained liversections of VASP −/− and WT animals E ) Histological images of platelet-neutrophil complexes (neutrophil = blue; platelet = black) in tissue sections of ischemic tissue of VASP −/− and WT animals (Data are shown as Mean ± SEM, n = 6, * P

    Journal: PLoS ONE

    Article Title: Phosphorylation of Vasodilator-Stimulated Phosphoprotein (VASP) Dampens Hepatic Ischemia-Reperfusion Injury

    doi: 10.1371/journal.pone.0029494

    Figure Lengend Snippet: VASP −/− animals demonstrate reduced hepatic IR injury. A ) LDH-serum levels of VASP −/− and WT mice after 30 minutes ischemia and 3 hours reperfusion in U/L. B ) Correlating serum levels of AST and C ) ALT of VASP −/− and WT animals. D ) Representative TTC stained liversections of VASP −/− and WT animals E ) Histological images of platelet-neutrophil complexes (neutrophil = blue; platelet = black) in tissue sections of ischemic tissue of VASP −/− and WT animals (Data are shown as Mean ± SEM, n = 6, * P

    Article Snippet: Tissue damage was determined through blood serum levels of lactate dehydrogenase (LDH) (Randox, Crumlin, United Kingdom), aspartate (AST) and alanine aminotransferase (ALT) (Teco Diagnostics, Anaheim, USA).

    Techniques: Mouse Assay, AST Assay, Staining

    Serum aminotransferase and arteriosclerosis levels. ( a ) AST and ALT index in serum; ( b ) AI index in serum. The data are presented as the means ± SD ( n = 10). (* ,# ) Significant difference at p

    Journal: Molecules

    Article Title: Therapeutic Effect of Chitooligosaccharide Tablets on Lipids in High-Fat Diets Induced Hyperlipidemic Rats

    doi: 10.3390/molecules24030514

    Figure Lengend Snippet: Serum aminotransferase and arteriosclerosis levels. ( a ) AST and ALT index in serum; ( b ) AI index in serum. The data are presented as the means ± SD ( n = 10). (* ,# ) Significant difference at p

    Article Snippet: TC, TG, HDL, LDL, Aspartate Aminotransferase (AST) and Alanine Aminotransferase (ALT) were measured using commercially-available kits (Biosino, Beijing, China).

    Techniques: AST Assay

    GL, rather than GA, prevents APAP-induced hepatocyte damage both in APAP-overdosed mice and APAP-treated LO2 cells. (A, B) GA exposure in serum (A) and in liver (B) in GA/APAP-treated mice and GL/APAP-treated mice. (C, D) Serum ALT and AST levels. (E) Cell viability of GL/APAP 20 mM-treated LO2 cells. (F) Cell viability of GA/APAP 20 mM-treated LO2 cells. (G) Cell viability of 18 β -GA/APAP 20 mM-treated LO2 cells. Data are expressed as mean ± S.E.M., n = 5–6 for both animal experiments and LO2 cell experiments. Control, saline-treated mice; GA, only GA 50 mg/kg-treated mice; GL, only GL 50 mg/kg-treated mice; APAP, saline/APAP-treated mice; GA + APAP, GA 50 mg/kg/APAP-treated mice; GL + APAP, GL 50 mg/kg/APAP-treated mice; ## P

    Journal: Drug Metabolism and Disposition

    Article Title: Glycyrrhizin Protects against Acetaminophen-Induced Acute Liver Injury via Alleviating Tumor Necrosis Factor α

    doi: 10.1124/dmd.116.069419

    Figure Lengend Snippet: GL, rather than GA, prevents APAP-induced hepatocyte damage both in APAP-overdosed mice and APAP-treated LO2 cells. (A, B) GA exposure in serum (A) and in liver (B) in GA/APAP-treated mice and GL/APAP-treated mice. (C, D) Serum ALT and AST levels. (E) Cell viability of GL/APAP 20 mM-treated LO2 cells. (F) Cell viability of GA/APAP 20 mM-treated LO2 cells. (G) Cell viability of 18 β -GA/APAP 20 mM-treated LO2 cells. Data are expressed as mean ± S.E.M., n = 5–6 for both animal experiments and LO2 cell experiments. Control, saline-treated mice; GA, only GA 50 mg/kg-treated mice; GL, only GL 50 mg/kg-treated mice; APAP, saline/APAP-treated mice; GA + APAP, GA 50 mg/kg/APAP-treated mice; GL + APAP, GL 50 mg/kg/APAP-treated mice; ## P

    Article Snippet: Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were quantified using a standard clinical automatic analyzer or a commercial ALT or AST assay kit (Catachem, Bridgeport, CT).

    Techniques: Mouse Assay, AST Assay

    Intragastric administration of GL fails to prevent APAP-induced hepatotoxicity. (A) H E-stained liver sections 24 hours after APAP challenge. Original magnification, 20×; black scale bar, 50 µ m. (B, C) Serum ALT and AST levels. (D, E) GL and GA levels in serum and liver. Control, saline-treated control mice; APAP, saline/APAP-treated mice; GL50 (po) + APAP, GL50 mg/kg (oral)/APAP-treated mice; GL100 (po) + APAP, GL100 mg/kg (oral)/APAP-treated mice; GL50 (ip) + APAP, GL50 mg/kg (i.p.)/APAP-treated mice. Data are expressed as mean ± S.E.M., n = 4–5 in each group. ### P

    Journal: Drug Metabolism and Disposition

    Article Title: Glycyrrhizin Protects against Acetaminophen-Induced Acute Liver Injury via Alleviating Tumor Necrosis Factor α

    doi: 10.1124/dmd.116.069419

    Figure Lengend Snippet: Intragastric administration of GL fails to prevent APAP-induced hepatotoxicity. (A) H E-stained liver sections 24 hours after APAP challenge. Original magnification, 20×; black scale bar, 50 µ m. (B, C) Serum ALT and AST levels. (D, E) GL and GA levels in serum and liver. Control, saline-treated control mice; APAP, saline/APAP-treated mice; GL50 (po) + APAP, GL50 mg/kg (oral)/APAP-treated mice; GL100 (po) + APAP, GL100 mg/kg (oral)/APAP-treated mice; GL50 (ip) + APAP, GL50 mg/kg (i.p.)/APAP-treated mice. Data are expressed as mean ± S.E.M., n = 4–5 in each group. ### P

    Article Snippet: Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were quantified using a standard clinical automatic analyzer or a commercial ALT or AST assay kit (Catachem, Bridgeport, CT).

    Techniques: Staining, AST Assay, Mouse Assay

    GL combats APAP-induced hepatotoxicity in both Ripk3 -null mice and their wild-type littermates. (A, B) H E-stained liver sections of (A) Ripk3 +/+ mice and (B) Ripk3 −/− mice. Original magnification, 20×; black scale bar, 50 µ m. (C, D) Serum ALT and AST levels. Data are expressed as mean ± S.E.M., n = 5–6 mice for APAP and GL + APAP group and n = 3 for control group. Control, saline-treated control mice; APAP, saline/APAP-treated mice; GL50 + APAP, GL 50 mg/kg (i.p.)/APAP-treated mice. All experiments in Ripk3 -null mice and their wild-type littermates were repeated twice. # P

    Journal: Drug Metabolism and Disposition

    Article Title: Glycyrrhizin Protects against Acetaminophen-Induced Acute Liver Injury via Alleviating Tumor Necrosis Factor α

    doi: 10.1124/dmd.116.069419

    Figure Lengend Snippet: GL combats APAP-induced hepatotoxicity in both Ripk3 -null mice and their wild-type littermates. (A, B) H E-stained liver sections of (A) Ripk3 +/+ mice and (B) Ripk3 −/− mice. Original magnification, 20×; black scale bar, 50 µ m. (C, D) Serum ALT and AST levels. Data are expressed as mean ± S.E.M., n = 5–6 mice for APAP and GL + APAP group and n = 3 for control group. Control, saline-treated control mice; APAP, saline/APAP-treated mice; GL50 + APAP, GL 50 mg/kg (i.p.)/APAP-treated mice. All experiments in Ripk3 -null mice and their wild-type littermates were repeated twice. # P

    Article Snippet: Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were quantified using a standard clinical automatic analyzer or a commercial ALT or AST assay kit (Catachem, Bridgeport, CT).

    Techniques: Mouse Assay, Staining, AST Assay

    GL pretreatment reduces serum transaminases, improves liver histology, and normalizes inflammation. (A, B) Serum ALT and AST levels. (C) H E staining of liver sections. Original magnification, 20×; black scale bar, 50 µ m. (D) Serum TNF α levels. (E–G), Relative mRNA level of TNF α , IL-1 β , and IL-6 ( n = 6–8 in each group). Control, saline-treated control mice; GL100, only GL 100 mg/kg-treated mice; APAP, saline/APAP-treated mice; GL50 + APAP, GL 50 mg/kg/APAP-treated mice; GL100 + APAP, GL 100 mg/kg/APAP-treated mice. Data are expressed as mean ± S.E.M. # P

    Journal: Drug Metabolism and Disposition

    Article Title: Glycyrrhizin Protects against Acetaminophen-Induced Acute Liver Injury via Alleviating Tumor Necrosis Factor α

    doi: 10.1124/dmd.116.069419

    Figure Lengend Snippet: GL pretreatment reduces serum transaminases, improves liver histology, and normalizes inflammation. (A, B) Serum ALT and AST levels. (C) H E staining of liver sections. Original magnification, 20×; black scale bar, 50 µ m. (D) Serum TNF α levels. (E–G), Relative mRNA level of TNF α , IL-1 β , and IL-6 ( n = 6–8 in each group). Control, saline-treated control mice; GL100, only GL 100 mg/kg-treated mice; APAP, saline/APAP-treated mice; GL50 + APAP, GL 50 mg/kg/APAP-treated mice; GL100 + APAP, GL 100 mg/kg/APAP-treated mice. Data are expressed as mean ± S.E.M. # P

    Article Snippet: Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were quantified using a standard clinical automatic analyzer or a commercial ALT or AST assay kit (Catachem, Bridgeport, CT).

    Techniques: AST Assay, Staining, Mouse Assay

    Intravenous injection of GL inhibits APAP-induced hepatotoxicity. (A) H E-stained liver sections 4 hours after APAP challenge. Original magnification, 20×; black scale bar, 50 µ m. (B, C) Serum ALT and AST levels. (D, E) GL and GA levels in serum and liver. Data are expressed as mean ± S.E.M., n = 5–8 in each group for all analyses. Control, saline-treated control mice; APAP, saline/APAP-treated mice; GL50 (iv) + APAP, GL 50 mg/kg (i.v.)/APAP-treated mice; GL100 (iv) + APAP, GL 100 mg/kg (i.v.)/APAP-treated mice; GL100 (iv), only GL 100 mg/kg (i.v.)-treated mice. ### P

    Journal: Drug Metabolism and Disposition

    Article Title: Glycyrrhizin Protects against Acetaminophen-Induced Acute Liver Injury via Alleviating Tumor Necrosis Factor α

    doi: 10.1124/dmd.116.069419

    Figure Lengend Snippet: Intravenous injection of GL inhibits APAP-induced hepatotoxicity. (A) H E-stained liver sections 4 hours after APAP challenge. Original magnification, 20×; black scale bar, 50 µ m. (B, C) Serum ALT and AST levels. (D, E) GL and GA levels in serum and liver. Data are expressed as mean ± S.E.M., n = 5–8 in each group for all analyses. Control, saline-treated control mice; APAP, saline/APAP-treated mice; GL50 (iv) + APAP, GL 50 mg/kg (i.v.)/APAP-treated mice; GL100 (iv) + APAP, GL 100 mg/kg (i.v.)/APAP-treated mice; GL100 (iv), only GL 100 mg/kg (i.v.)-treated mice. ### P

    Article Snippet: Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were quantified using a standard clinical automatic analyzer or a commercial ALT or AST assay kit (Catachem, Bridgeport, CT).

    Techniques: Injection, Staining, AST Assay, Mouse Assay

    Intraperitoneal injection of GL decreases serum ALT and AST level at the early stage after APAP challenge. (A) Mouse experiment procedure scheme. (B, C) Time course of serum ALT and AST levels after APAP challenge. (D, E) Pharmacokinetic distribution of GL and GA in serum (D) and liver (E) in GL100 (ip)/APAP-treated mice. Data are expressed as mean ± S.E.M., n = 3 in each group for control mice and n = 6 for other groups. # P

    Journal: Drug Metabolism and Disposition

    Article Title: Glycyrrhizin Protects against Acetaminophen-Induced Acute Liver Injury via Alleviating Tumor Necrosis Factor α

    doi: 10.1124/dmd.116.069419

    Figure Lengend Snippet: Intraperitoneal injection of GL decreases serum ALT and AST level at the early stage after APAP challenge. (A) Mouse experiment procedure scheme. (B, C) Time course of serum ALT and AST levels after APAP challenge. (D, E) Pharmacokinetic distribution of GL and GA in serum (D) and liver (E) in GL100 (ip)/APAP-treated mice. Data are expressed as mean ± S.E.M., n = 3 in each group for control mice and n = 6 for other groups. # P

    Article Snippet: Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were quantified using a standard clinical automatic analyzer or a commercial ALT or AST assay kit (Catachem, Bridgeport, CT).

    Techniques: Injection, AST Assay, Mouse Assay

    GL markedly attenuates serum ALT and AST levels in both wild-type and Cyp2e1 -null mice. (A, B) Serum ALT and AST levels in wild-type mice. (C, D) Serum ALT and AST levels in Cyp2e1 null mice. Control, saline-treated mice; GL 50, only GL 50 mg/kg-treated mice; APAP, saline/APAP-treated mice; GL50 + APAP, GL 50 mg/kg (i.p.)/APAP-treated mice. WT, wild-type. Data are expressed as mean ± S.E.M., n = 5 mice in each group. # P

    Journal: Drug Metabolism and Disposition

    Article Title: Glycyrrhizin Protects against Acetaminophen-Induced Acute Liver Injury via Alleviating Tumor Necrosis Factor α

    doi: 10.1124/dmd.116.069419

    Figure Lengend Snippet: GL markedly attenuates serum ALT and AST levels in both wild-type and Cyp2e1 -null mice. (A, B) Serum ALT and AST levels in wild-type mice. (C, D) Serum ALT and AST levels in Cyp2e1 null mice. Control, saline-treated mice; GL 50, only GL 50 mg/kg-treated mice; APAP, saline/APAP-treated mice; GL50 + APAP, GL 50 mg/kg (i.p.)/APAP-treated mice. WT, wild-type. Data are expressed as mean ± S.E.M., n = 5 mice in each group. # P

    Article Snippet: Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were quantified using a standard clinical automatic analyzer or a commercial ALT or AST assay kit (Catachem, Bridgeport, CT).

    Techniques: AST Assay, Mouse Assay

    The correlation of values and hepatic necroinflammatory activity with biopsy. The correlations of values ( a ALT, b GGT, c PT, d ALB, e H value) and hepatic necroinflammatory activity with biopsy are shown in box plots. The top and bottom of each box represents the 25th and 75th centile intervals. The line through the box in the median

    Journal: Journal of Translational Medicine

    Article Title: Prediction of hepatic necroinflammatory activity in patients with chronic hepatitis B by a simple noninvasive model

    doi: 10.1186/s12967-018-1538-z

    Figure Lengend Snippet: The correlation of values and hepatic necroinflammatory activity with biopsy. The correlations of values ( a ALT, b GGT, c PT, d ALB, e H value) and hepatic necroinflammatory activity with biopsy are shown in box plots. The top and bottom of each box represents the 25th and 75th centile intervals. The line through the box in the median

    Article Snippet: The reference value was 5–50 IU/L for alanine aminotransferase (ALT) (IFCC, 37 °C), 15–60 IU/L for GGT, 40–55 g/L for ALB, and 9–13 s for prothrombin time (PT).

    Techniques: Activity Assay

    ROC curves of the model in the validation cohort. The AUROC of the H index, ALT, GGT, ALB, and PT was 0.847 (95% CI 0.811–0.883, P

    Journal: Journal of Translational Medicine

    Article Title: Prediction of hepatic necroinflammatory activity in patients with chronic hepatitis B by a simple noninvasive model

    doi: 10.1186/s12967-018-1538-z

    Figure Lengend Snippet: ROC curves of the model in the validation cohort. The AUROC of the H index, ALT, GGT, ALB, and PT was 0.847 (95% CI 0.811–0.883, P

    Article Snippet: The reference value was 5–50 IU/L for alanine aminotransferase (ALT) (IFCC, 37 °C), 15–60 IU/L for GGT, 40–55 g/L for ALB, and 9–13 s for prothrombin time (PT).

    Techniques:

    The additional contribution of the AST-to-ALT ratio to predicting the risk of metabolic syndrome.

    Journal: PLoS ONE

    Article Title: Incremental Predictive Value of Serum AST-to-ALT Ratio for Incident Metabolic Syndrome: The ARIRANG Study

    doi: 10.1371/journal.pone.0161304

    Figure Lengend Snippet: The additional contribution of the AST-to-ALT ratio to predicting the risk of metabolic syndrome.

    Article Snippet: Blood glucose, serum HDL-cholesterol, triglyceride, LDL-cholesterol (LDL-C), very low density lipoprotein (VLDL) cholesterol, aspartate transaminase (AST), and alanine transaminase (ALT) were measured by enzymatic methods (ADVIA 1650, Siemens, Tarrytown, NY, USA).

    Techniques: AST Assay

    Transplantation of OSKP-iPSC-Heps alleviated steatohepatitis in 18-month-old male MCD diet-fed mouse ( A ) Time course of the changes in serum ALT levels among control diet-fed mice, MCDD-fed mice, and MCDD-fed mice with intrasplenic transplantation of OSKP-iPSC-Heps or SnHeps. ( B ) H E staining showing the fat accumulation and severe hepatocyte vacuolization over the liver from MCDD-fed mice. Effect of intrasplenic transplantation of OSKP-iPSC-Heps or SnHeps on serum ( C ) ALT and ( D ) AST levels, and ( E ) hepatic triglyceride accumulation in MCDD-fed mice. In Panels C, D and E, * P

    Journal: Oncotarget

    Article Title: Improvement of non-alcoholic steatohepatitis by hepatocyte-like cells generated from iPSCs with Oct4/Sox2/Klf4/Parp1

    doi: 10.18632/oncotarget.23603

    Figure Lengend Snippet: Transplantation of OSKP-iPSC-Heps alleviated steatohepatitis in 18-month-old male MCD diet-fed mouse ( A ) Time course of the changes in serum ALT levels among control diet-fed mice, MCDD-fed mice, and MCDD-fed mice with intrasplenic transplantation of OSKP-iPSC-Heps or SnHeps. ( B ) H E staining showing the fat accumulation and severe hepatocyte vacuolization over the liver from MCDD-fed mice. Effect of intrasplenic transplantation of OSKP-iPSC-Heps or SnHeps on serum ( C ) ALT and ( D ) AST levels, and ( E ) hepatic triglyceride accumulation in MCDD-fed mice. In Panels C, D and E, * P

    Article Snippet: Serum ammonia and biochemical parameters, including alanine aminotransferase (ALT), aspartate aminotransferase (AST), and total bilirubin (TBIL) were analyzed using a Vitro DT chemistry system (Johnson & Johnson).

    Techniques: Transplantation Assay, Mouse Assay, Staining, AST Assay