alanine aminotransferase alt Search Results


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  • 95
    Millipore alanine transaminase aminotransferase alt activity
    LPA protects against experimental APAP-induced acute liver injury. ( a – c ) After fasting for 16 h, APAP (400 mg kg −1 ) was administered to mice. Vehicle (PBS containing 1% DMSO) or LPA was injected 30 min before the APAP treatment. The mice were killed at 0.5, 1, 3, 6 and 12 h after the treatment, and their livers were stained with hematoxylin and eosin (magnification: × 200) ( a ). The serum levels of <t>AST</t> and <t>ALT</t> in the mice were measured by enzyme activity assays ( b ). Necrotic cells were visualized by TUNEL histology (magnification: × 200) and enumerated ( c ). ( d , e ) For the survival rate, the mice were administered APAP (750 mg kg −1 ) at 30 min after intraperitoneal injection with the vehicle (PBS containing 1% DMSO), LPA (4 mg kg −1 ) or NAC (150 mg kg −1 ). The survival rate was monitored every 6 h for 72 h ( d , e ). The data are expressed as the mean±s.e.m. ( n =5 for b , c , right). * P
    Alanine Transaminase Aminotransferase Alt Activity, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore alanine aminotransferase alt
    The role of S1PR2 in BDL-induced increase of bile acid levels and fibrotic liver injury Wild type and S1PR2 −/− mice were subjected to 2-week BDL or sham operation as described in “Methods”. The bile acids, <t>AST,</t> <t>ALT,</t> ALP and hydroxyproline levels in serum and liver were measured using commercial kits according to the manufacturer’s instructions. (A) Total bile acids in serum. (B) Total bile acids in liver, normalized with liver weight and expressed as μM/mg. (C) AST activity in serum. (D) ALT activity in Serum. (E) ALP activity in serum. (F) Hydroxyproline in liver. Statistical significance relative to sham control, * p
    Alanine Aminotransferase Alt, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 226 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alpha Laboratories alanine transaminase alt
    The role of S1PR2 in BDL-induced increase of bile acid levels and fibrotic liver injury Wild type and S1PR2 −/− mice were subjected to 2-week BDL or sham operation as described in “Methods”. The bile acids, <t>AST,</t> <t>ALT,</t> ALP and hydroxyproline levels in serum and liver were measured using commercial kits according to the manufacturer’s instructions. (A) Total bile acids in serum. (B) Total bile acids in liver, normalized with liver weight and expressed as μM/mg. (C) AST activity in serum. (D) ALT activity in Serum. (E) ALP activity in serum. (F) Hydroxyproline in liver. Statistical significance relative to sham control, * p
    Alanine Transaminase Alt, supplied by Alpha Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioVision alanine aminotransferase alt
    The role of S1PR2 in BDL-induced increase of bile acid levels and fibrotic liver injury Wild type and S1PR2 −/− mice were subjected to 2-week BDL or sham operation as described in “Methods”. The bile acids, <t>AST,</t> <t>ALT,</t> ALP and hydroxyproline levels in serum and liver were measured using commercial kits according to the manufacturer’s instructions. (A) Total bile acids in serum. (B) Total bile acids in liver, normalized with liver weight and expressed as μM/mg. (C) AST activity in serum. (D) ALT activity in Serum. (E) ALP activity in serum. (F) Hydroxyproline in liver. Statistical significance relative to sham control, * p
    Alanine Aminotransferase Alt, supplied by BioVision, used in various techniques. Bioz Stars score: 92/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Siemens AG alanine transaminase alt
    Mean postoperative changes in serum <t>AST</t> (A) and <t>ALT</t> (B). Changes from day of surgery (D0) to Day 6 after surgery (D6) in the septic (S) and nonseptic (NS) groups. There are significant differences between the S and NS groups from as early as the day after surgery. * indicates p
    Alanine Transaminase Alt, supplied by Siemens AG, used in various techniques. Bioz Stars score: 92/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Fuji Pharma alanine transaminase alt
    Mean postoperative changes in serum <t>AST</t> (A) and <t>ALT</t> (B). Changes from day of surgery (D0) to Day 6 after surgery (D6) in the septic (S) and nonseptic (NS) groups. There are significant differences between the S and NS groups from as early as the day after surgery. * indicates p
    Alanine Transaminase Alt, supplied by Fuji Pharma, used in various techniques. Bioz Stars score: 92/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam alanine aminotransferase alt
    In vivo antitumor activity, pharmacokinetics and safety of Db10-Glyco-scTRAIL-FAVS G AA. (A) Colo205-bearing nude mice received 8 i.v. injections of the Db10-Glyco-scTRAIL-FAVS G AA fusion protein (0.1 nmol, 0.3 nmol, or 1.0 nmol) in combination with 8 intraperitoneal (i.p.) injections of bortezomib (Brt; 5µg per injection) every day indicated by dots. Mean ± 95% CI (n = 12 tumors per group). The 1.0 nmol-treated group received an identical regime of Db10-Glyco-scTRAIL-FAVS G AA and bortezomib after regrowth of tumors (volume ∼100 mm 3 ). Therefore, animals were divided into 2 subgroups of fast (I) and slow (II) regrowth. (B, C) Db10-Glyco-scTRAIL-FAVS G AA (168 µg per animal in B / 25 µg per animal in C) was injected i.v. into Colo205-bearing nude mice (B) or CD-1 mice (C). The serum concentrations of the fusion protein were analyzed via ELISA. Mean ± SD (n = 3). (D, E) Activity of alanine aminotransferase <t>(ALT,</t> D) and <t>α-amylase</t> (E) was measured after 4 hours (only for D), 1 day, and 9 d after the first of total 8 injections (every day) of Db10-Glyco-scTRAIL-FAVS G AA (0.1 nmol, 0.3 nmol, or 1.0 nmol; i.v.) and/or bortezomib (Brt, 5 µg; i.p.). Control mice were non-treated. Mean ± SD (n = 3).
    Alanine Aminotransferase Alt, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Randox alanine transaminase alt kits
    ABAH, an inhibitor of MPO enzyme activity, alleviates iron-induced toxicity. Eight weeks old female WT mice were injected Fe2+ (25 mg/kg of body weight; ferrous sulfate), intraperitoneally or sterile saline as vehicle. These mice were pretreated with 40 mg/kg of ABAH in PBS or vehicle control 1h before and 12h after iron administration. At 24h post iron injection, mice were monitored for body weight (A). Serum MPO (B), <t>AST</t> (C), <t>ALT</t> (D), ELA2 (E), Lcn2 (F), SAA (G), and KC (H) levels were measured. Values are means ± SEM, n=4–5. Bar without a common letter differ. p
    Alanine Transaminase Alt Kits, supplied by Randox, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher alanine transaminase alt activity
    ABAH, an inhibitor of MPO enzyme activity, alleviates iron-induced toxicity. Eight weeks old female WT mice were injected Fe2+ (25 mg/kg of body weight; ferrous sulfate), intraperitoneally or sterile saline as vehicle. These mice were pretreated with 40 mg/kg of ABAH in PBS or vehicle control 1h before and 12h after iron administration. At 24h post iron injection, mice were monitored for body weight (A). Serum MPO (B), <t>AST</t> (C), <t>ALT</t> (D), ELA2 (E), Lcn2 (F), SAA (G), and KC (H) levels were measured. Values are means ± SEM, n=4–5. Bar without a common letter differ. p
    Alanine Transaminase Alt Activity, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore liver associated enzyme alanine transaminase alt
    ABAH, an inhibitor of MPO enzyme activity, alleviates iron-induced toxicity. Eight weeks old female WT mice were injected Fe2+ (25 mg/kg of body weight; ferrous sulfate), intraperitoneally or sterile saline as vehicle. These mice were pretreated with 40 mg/kg of ABAH in PBS or vehicle control 1h before and 12h after iron administration. At 24h post iron injection, mice were monitored for body weight (A). Serum MPO (B), <t>AST</t> (C), <t>ALT</t> (D), ELA2 (E), Lcn2 (F), SAA (G), and KC (H) levels were measured. Values are means ± SEM, n=4–5. Bar without a common letter differ. p
    Liver Associated Enzyme Alanine Transaminase Alt, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Roche alanine transaminase alt
    Retrospective comparison of organ function between dying and surviving septic mice using CLP as reference point. Plasma levels of (A) <t>ALT</t> (B) <t>LDH</t> (C) urea and (D) glucose in mice that died (CLP-Only DIED) or survived (CLP-Only SUR) post-CLP were compared to surviving CLP-ODam mice (CLP-ODam SUR). For (A) to (D) in CLP-Only: at 6 h, DIED n = 45 and SUR n ≥ 36; at 24 h, DIED n = 37 and SUR n = 38; at 48 h, DIED n = 13 and SUR n ≥ 37; at 72 h, DIED n = 7 and SUR n ≥ 34; at 96 h, DIED n = 3, SUR n = 36. In CLP-ODam SUR n = 3 at all measured time points (24, 48, 72 h); * p
    Alanine Transaminase Alt, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 357 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Beckman Coulter alanine aminotransferase alt
    PS-ASOs induce hepatotoxicity in mice in a sugar modification-dependent manner. ( A ) Schematic representation of the 5–10–5 Pten gapmer ASO. ( B ) Plasma levels of <t>ALT</t> and <t>AST</t> upon a single administration of Pten PS-ASOs modified with 2′ MOE (ION-116847), cEt (ION-582801) or 2′ F (ION-404130) at 100 or 400 mg/kg for 96 h. N = 3 per group. ( C ) Plasma levels of ALT and AST upon a single administration of Pten LNA (ION-390896) at 100 or 400 mg/kg for 96 h. N = 3 per group. ( D ) Plasma levels of ALT and AST upon repeated administration of 2′ F ASO (ION-404130) at 20 mg/kg per dose, three doses per week. S-saline, and F-2′ F PS-ASO (ION-404130). ( E ) qRT-PCR of on-target Pten mRNA. Mice were treated as was described in B. The error bars indicate data acquired from three individual animals. ( F ) qRT-PCR showed dose-dependent reduction of target Pten mRNA in mouse hepatocytes by Pten ASOs modified with 2′ MOE (ION-116847), cEt (ION-582801), or 2′ F (ION-404130). ASOs were delivered by lipid transfection. The error bars represent standard deviation from three separate experiments. ( G ) Plasma levels of ALT and AST upon a single administration of PS-ASOs modified with either 2′ MOE or 2′ F at 400 mg/kg for 96 h. Seven pairs of 2′ MOE or 2′ F-modified sequences were included. Hepatotoxic potentials of the 2′ F PS-ASOs were rank-ordered and color-coded (red: high hepatotoxic potentials; and green: low hepatotoxic potentials) based on plasma levels of ALT and AST. N = 3 per group. F.D. = found death.
    Alanine Aminotransferase Alt, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 94/100, based on 653 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Hitachi Ltd alanine aminotransferase alt
    PZH has no significant toxicity in EAE rats. (a) The organ coefficient of several organs from the respective group was detected. The levels of <t>ALT</t> (b) and <t>AST</t> (c) for hepatotoxicity as well as CREA (d) and UREA (e) for nephrotoxicity were shown, respectively.
    Alanine Aminotransferase Alt, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 93/100, based on 1325 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Randox alanine transaminase alt
    Decoction of Hedyotis diffusa (HD) relieves the acute inflammation in hepatitis mouse. a Administration of HD decoction blocked LPS/GALN-induced up-regulation of mRNA levels of CXCL1, TNF-α, IL-1β, MIP-2. b The treatment of HD decoction reduced the expressed levels of TNF-α, IL-1β, IL-6 and MCP-1 in liver of LPS/GALN-injected mice. c Alanine transaminase <t>(ALT)</t> level was measured in control or LPS/GALN- and LPS/GALN + HD-treated mouse. HD decoction significantly reduced the level of ALT (n = 5). d Aspartate transaminase <t>(AST)</t> level was measured in control or LPS/GALN- and LPS/GALN + HD-treated mouse. HD decoction significantly reduced the level of AST (n = 5). e Survival of hepatitis mouse was measured after treatment of HD decoction (n = 20, per group) within 20 days. f Effect of HD on LPS/GALN-mediated liver histopathologic changes
    Alanine Transaminase Alt, supplied by Randox, used in various techniques. Bioz Stars score: 92/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stanbio alanine aminotransferase alt
    Effects of the FoxO1 inhibitor (AS) and APN on serum <t>AST,</t> <t>ALT,</t> and TG. Serum AST, ALT, and TG were determined by kits (Stanbio Laboratory, TX, USA). Values are expressed as mean ± SD, n = 6/group. ∗ P
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    LPA protects against experimental APAP-induced acute liver injury. ( a – c ) After fasting for 16 h, APAP (400 mg kg −1 ) was administered to mice. Vehicle (PBS containing 1% DMSO) or LPA was injected 30 min before the APAP treatment. The mice were killed at 0.5, 1, 3, 6 and 12 h after the treatment, and their livers were stained with hematoxylin and eosin (magnification: × 200) ( a ). The serum levels of AST and ALT in the mice were measured by enzyme activity assays ( b ). Necrotic cells were visualized by TUNEL histology (magnification: × 200) and enumerated ( c ). ( d , e ) For the survival rate, the mice were administered APAP (750 mg kg −1 ) at 30 min after intraperitoneal injection with the vehicle (PBS containing 1% DMSO), LPA (4 mg kg −1 ) or NAC (150 mg kg −1 ). The survival rate was monitored every 6 h for 72 h ( d , e ). The data are expressed as the mean±s.e.m. ( n =5 for b , c , right). * P

    Journal: Experimental & Molecular Medicine

    Article Title: Lysophosphatidic acid protects against acetaminophen-induced acute liver injury

    doi: 10.1038/emm.2017.203

    Figure Lengend Snippet: LPA protects against experimental APAP-induced acute liver injury. ( a – c ) After fasting for 16 h, APAP (400 mg kg −1 ) was administered to mice. Vehicle (PBS containing 1% DMSO) or LPA was injected 30 min before the APAP treatment. The mice were killed at 0.5, 1, 3, 6 and 12 h after the treatment, and their livers were stained with hematoxylin and eosin (magnification: × 200) ( a ). The serum levels of AST and ALT in the mice were measured by enzyme activity assays ( b ). Necrotic cells were visualized by TUNEL histology (magnification: × 200) and enumerated ( c ). ( d , e ) For the survival rate, the mice were administered APAP (750 mg kg −1 ) at 30 min after intraperitoneal injection with the vehicle (PBS containing 1% DMSO), LPA (4 mg kg −1 ) or NAC (150 mg kg −1 ). The survival rate was monitored every 6 h for 72 h ( d , e ). The data are expressed as the mean±s.e.m. ( n =5 for b , c , right). * P

    Article Snippet: Measurement of serum alanine transaminase (ALT) and aspartate aminotransferase (AST) ALT and AST levels were measured using ALT and AST activity assay kits (Sigma) according to the manufacturer’s instructions.

    Techniques: Mouse Assay, Injection, Staining, AST Assay, Activity Assay, TUNEL Assay

    The role of S1PR2 in BDL-induced increase of bile acid levels and fibrotic liver injury Wild type and S1PR2 −/− mice were subjected to 2-week BDL or sham operation as described in “Methods”. The bile acids, AST, ALT, ALP and hydroxyproline levels in serum and liver were measured using commercial kits according to the manufacturer’s instructions. (A) Total bile acids in serum. (B) Total bile acids in liver, normalized with liver weight and expressed as μM/mg. (C) AST activity in serum. (D) ALT activity in Serum. (E) ALP activity in serum. (F) Hydroxyproline in liver. Statistical significance relative to sham control, * p

    Journal: Hepatology (Baltimore, Md.)

    Article Title: The role of S1PR2 in bile acid-induced cholangiocyte proliferation and cholestasis-induced liver injury in mice

    doi: 10.1002/hep.29076

    Figure Lengend Snippet: The role of S1PR2 in BDL-induced increase of bile acid levels and fibrotic liver injury Wild type and S1PR2 −/− mice were subjected to 2-week BDL or sham operation as described in “Methods”. The bile acids, AST, ALT, ALP and hydroxyproline levels in serum and liver were measured using commercial kits according to the manufacturer’s instructions. (A) Total bile acids in serum. (B) Total bile acids in liver, normalized with liver weight and expressed as μM/mg. (C) AST activity in serum. (D) ALT activity in Serum. (E) ALP activity in serum. (F) Hydroxyproline in liver. Statistical significance relative to sham control, * p

    Article Snippet: The activities of the plasma aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) were measured using assay kits from Sigma (St. Louis, MO).

    Techniques: Mouse Assay, AST Assay, ALP Assay, Activity Assay

    Mean postoperative changes in serum AST (A) and ALT (B). Changes from day of surgery (D0) to Day 6 after surgery (D6) in the septic (S) and nonseptic (NS) groups. There are significant differences between the S and NS groups from as early as the day after surgery. * indicates p

    Journal: BMC Anesthesiology

    Article Title: Early diagnostic markers of sepsis after oesophagectomy (including thromboelastography)

    doi: 10.1186/1471-2253-12-12

    Figure Lengend Snippet: Mean postoperative changes in serum AST (A) and ALT (B). Changes from day of surgery (D0) to Day 6 after surgery (D6) in the septic (S) and nonseptic (NS) groups. There are significant differences between the S and NS groups from as early as the day after surgery. * indicates p

    Article Snippet: Samples were analysed for: PCT (Elecsys BRAHMS PCT; Roche Diagnostics, Mannheim, Germany); IL- 6 (EIA IL-6; IMMUNOTECH, Marseille, France); lactate (Cobas Integra 400+; Roche, Switzerland); CRP, aspartate transaminase (AST) and alanine transaminase (ALT) (all Advia 1800; Siemens, USA).

    Techniques: AST Assay

    In vivo antitumor activity, pharmacokinetics and safety of Db10-Glyco-scTRAIL-FAVS G AA. (A) Colo205-bearing nude mice received 8 i.v. injections of the Db10-Glyco-scTRAIL-FAVS G AA fusion protein (0.1 nmol, 0.3 nmol, or 1.0 nmol) in combination with 8 intraperitoneal (i.p.) injections of bortezomib (Brt; 5µg per injection) every day indicated by dots. Mean ± 95% CI (n = 12 tumors per group). The 1.0 nmol-treated group received an identical regime of Db10-Glyco-scTRAIL-FAVS G AA and bortezomib after regrowth of tumors (volume ∼100 mm 3 ). Therefore, animals were divided into 2 subgroups of fast (I) and slow (II) regrowth. (B, C) Db10-Glyco-scTRAIL-FAVS G AA (168 µg per animal in B / 25 µg per animal in C) was injected i.v. into Colo205-bearing nude mice (B) or CD-1 mice (C). The serum concentrations of the fusion protein were analyzed via ELISA. Mean ± SD (n = 3). (D, E) Activity of alanine aminotransferase (ALT, D) and α-amylase (E) was measured after 4 hours (only for D), 1 day, and 9 d after the first of total 8 injections (every day) of Db10-Glyco-scTRAIL-FAVS G AA (0.1 nmol, 0.3 nmol, or 1.0 nmol; i.v.) and/or bortezomib (Brt, 5 µg; i.p.). Control mice were non-treated. Mean ± SD (n = 3).

    Journal: mAbs

    Article Title: An optimized antibody-single-chain TRAIL fusion protein for cancer therapy

    doi: 10.1080/19420862.2016.1172163

    Figure Lengend Snippet: In vivo antitumor activity, pharmacokinetics and safety of Db10-Glyco-scTRAIL-FAVS G AA. (A) Colo205-bearing nude mice received 8 i.v. injections of the Db10-Glyco-scTRAIL-FAVS G AA fusion protein (0.1 nmol, 0.3 nmol, or 1.0 nmol) in combination with 8 intraperitoneal (i.p.) injections of bortezomib (Brt; 5µg per injection) every day indicated by dots. Mean ± 95% CI (n = 12 tumors per group). The 1.0 nmol-treated group received an identical regime of Db10-Glyco-scTRAIL-FAVS G AA and bortezomib after regrowth of tumors (volume ∼100 mm 3 ). Therefore, animals were divided into 2 subgroups of fast (I) and slow (II) regrowth. (B, C) Db10-Glyco-scTRAIL-FAVS G AA (168 µg per animal in B / 25 µg per animal in C) was injected i.v. into Colo205-bearing nude mice (B) or CD-1 mice (C). The serum concentrations of the fusion protein were analyzed via ELISA. Mean ± SD (n = 3). (D, E) Activity of alanine aminotransferase (ALT, D) and α-amylase (E) was measured after 4 hours (only for D), 1 day, and 9 d after the first of total 8 injections (every day) of Db10-Glyco-scTRAIL-FAVS G AA (0.1 nmol, 0.3 nmol, or 1.0 nmol; i.v.) and/or bortezomib (Brt, 5 µg; i.p.). Control mice were non-treated. Mean ± SD (n = 3).

    Article Snippet: The activity of alanine aminotransferase (ALT) and α-amylase were measured using an enzymatic assay kit (abcam) according to the manufacturer's instructions.

    Techniques: In Vivo, Activity Assay, Mouse Assay, Injection, Enzyme-linked Immunosorbent Assay

    ABAH, an inhibitor of MPO enzyme activity, alleviates iron-induced toxicity. Eight weeks old female WT mice were injected Fe2+ (25 mg/kg of body weight; ferrous sulfate), intraperitoneally or sterile saline as vehicle. These mice were pretreated with 40 mg/kg of ABAH in PBS or vehicle control 1h before and 12h after iron administration. At 24h post iron injection, mice were monitored for body weight (A). Serum MPO (B), AST (C), ALT (D), ELA2 (E), Lcn2 (F), SAA (G), and KC (H) levels were measured. Values are means ± SEM, n=4–5. Bar without a common letter differ. p

    Journal: The Journal of nutritional biochemistry

    Article Title: Myeloperoxidase Deficiency Attenuates Systemic and Dietary Iron-induced Adverse Effects

    doi: 10.1016/j.jnutbio.2018.08.003

    Figure Lengend Snippet: ABAH, an inhibitor of MPO enzyme activity, alleviates iron-induced toxicity. Eight weeks old female WT mice were injected Fe2+ (25 mg/kg of body weight; ferrous sulfate), intraperitoneally or sterile saline as vehicle. These mice were pretreated with 40 mg/kg of ABAH in PBS or vehicle control 1h before and 12h after iron administration. At 24h post iron injection, mice were monitored for body weight (A). Serum MPO (B), AST (C), ALT (D), ELA2 (E), Lcn2 (F), SAA (G), and KC (H) levels were measured. Values are means ± SEM, n=4–5. Bar without a common letter differ. p

    Article Snippet: Aspartate transaminase (AST) and alanine transaminase (ALT) kits were purchased from Randox Laboratories.

    Techniques: Activity Assay, Mouse Assay, Injection, AST Assay

    MPO deficiency protects against iron-induced adverse effects. Eight weeks old male M po KO mice and their WT littermates were injected intraperitoneally Fe2+ (25 mg/kg of body weight; ferrous sulfate) or sterile saline as vehicle. After 24h, mice were monitored for body weights (A). Serum was collected to measure levels of MPO (B), AST (C), ALT (D), ELA2 (E), Lcn2 (F), SAA (G), and KC (H). Data presented are means ± SEM, n=5–6. Bar without a common letter differ. p

    Journal: The Journal of nutritional biochemistry

    Article Title: Myeloperoxidase Deficiency Attenuates Systemic and Dietary Iron-induced Adverse Effects

    doi: 10.1016/j.jnutbio.2018.08.003

    Figure Lengend Snippet: MPO deficiency protects against iron-induced adverse effects. Eight weeks old male M po KO mice and their WT littermates were injected intraperitoneally Fe2+ (25 mg/kg of body weight; ferrous sulfate) or sterile saline as vehicle. After 24h, mice were monitored for body weights (A). Serum was collected to measure levels of MPO (B), AST (C), ALT (D), ELA2 (E), Lcn2 (F), SAA (G), and KC (H). Data presented are means ± SEM, n=5–6. Bar without a common letter differ. p

    Article Snippet: Aspartate transaminase (AST) and alanine transaminase (ALT) kits were purchased from Randox Laboratories.

    Techniques: Mouse Assay, Injection, AST Assay

    Retrospective comparison of organ function between dying and surviving septic mice using CLP as reference point. Plasma levels of (A) ALT (B) LDH (C) urea and (D) glucose in mice that died (CLP-Only DIED) or survived (CLP-Only SUR) post-CLP were compared to surviving CLP-ODam mice (CLP-ODam SUR). For (A) to (D) in CLP-Only: at 6 h, DIED n = 45 and SUR n ≥ 36; at 24 h, DIED n = 37 and SUR n = 38; at 48 h, DIED n = 13 and SUR n ≥ 37; at 72 h, DIED n = 7 and SUR n ≥ 34; at 96 h, DIED n = 3, SUR n = 36. In CLP-ODam SUR n = 3 at all measured time points (24, 48, 72 h); * p

    Journal: Intensive Care Medicine Experimental

    Article Title: Why do they die? Comparison of selected aspects of organ injury and dysfunction in mice surviving and dying in acute abdominal sepsis

    doi: 10.1186/s40635-015-0048-z

    Figure Lengend Snippet: Retrospective comparison of organ function between dying and surviving septic mice using CLP as reference point. Plasma levels of (A) ALT (B) LDH (C) urea and (D) glucose in mice that died (CLP-Only DIED) or survived (CLP-Only SUR) post-CLP were compared to surviving CLP-ODam mice (CLP-ODam SUR). For (A) to (D) in CLP-Only: at 6 h, DIED n = 45 and SUR n ≥ 36; at 24 h, DIED n = 37 and SUR n = 38; at 48 h, DIED n = 13 and SUR n ≥ 37; at 72 h, DIED n = 7 and SUR n ≥ 34; at 96 h, DIED n = 3, SUR n = 36. In CLP-ODam SUR n = 3 at all measured time points (24, 48, 72 h); * p

    Article Snippet: Metabolic and organ injury/function parameters In the first experiment (28 day follow-up), urea nitrogen (urea), glucose, lactate dehydrogenase (LDH) and alanine transaminase (ALT) were analyzed in plasma samples with Cobas c111 analyzer (Roche, Basel, Switzerland).

    Techniques: Mouse Assay

    Retrospective comparison of organ function between dying and surviving septic mice using death as reference point . Plasma levels of (A) ALT, (B) LDH, (C) urea and (D ) glucose in mice subjected to CLP were compared between those that either died (CLP-Only DIED) or survived (CLP-Only SUR) and additionally to surviving CLP-ODam mice (CLP-ODam SUR). For (A) to (D) in the CLP-Only group: at 72 h, DIED n = 9 and SUR n = 35; at 48 h, DIED n = 33 and SUR n ≥ 30; at 24 h, DIED n = 42 and SUR n = 40. In the CLP-ODam SUR group, an average value of all combines measurements (i.e. taken at 24, 48 and 72 h post-CLP; n = 9) is shown at the 24 h prior death time point; * p

    Journal: Intensive Care Medicine Experimental

    Article Title: Why do they die? Comparison of selected aspects of organ injury and dysfunction in mice surviving and dying in acute abdominal sepsis

    doi: 10.1186/s40635-015-0048-z

    Figure Lengend Snippet: Retrospective comparison of organ function between dying and surviving septic mice using death as reference point . Plasma levels of (A) ALT, (B) LDH, (C) urea and (D ) glucose in mice subjected to CLP were compared between those that either died (CLP-Only DIED) or survived (CLP-Only SUR) and additionally to surviving CLP-ODam mice (CLP-ODam SUR). For (A) to (D) in the CLP-Only group: at 72 h, DIED n = 9 and SUR n = 35; at 48 h, DIED n = 33 and SUR n ≥ 30; at 24 h, DIED n = 42 and SUR n = 40. In the CLP-ODam SUR group, an average value of all combines measurements (i.e. taken at 24, 48 and 72 h post-CLP; n = 9) is shown at the 24 h prior death time point; * p

    Article Snippet: Metabolic and organ injury/function parameters In the first experiment (28 day follow-up), urea nitrogen (urea), glucose, lactate dehydrogenase (LDH) and alanine transaminase (ALT) were analyzed in plasma samples with Cobas c111 analyzer (Roche, Basel, Switzerland).

    Techniques: Mouse Assay

    PS-ASOs induce hepatotoxicity in mice in a sugar modification-dependent manner. ( A ) Schematic representation of the 5–10–5 Pten gapmer ASO. ( B ) Plasma levels of ALT and AST upon a single administration of Pten PS-ASOs modified with 2′ MOE (ION-116847), cEt (ION-582801) or 2′ F (ION-404130) at 100 or 400 mg/kg for 96 h. N = 3 per group. ( C ) Plasma levels of ALT and AST upon a single administration of Pten LNA (ION-390896) at 100 or 400 mg/kg for 96 h. N = 3 per group. ( D ) Plasma levels of ALT and AST upon repeated administration of 2′ F ASO (ION-404130) at 20 mg/kg per dose, three doses per week. S-saline, and F-2′ F PS-ASO (ION-404130). ( E ) qRT-PCR of on-target Pten mRNA. Mice were treated as was described in B. The error bars indicate data acquired from three individual animals. ( F ) qRT-PCR showed dose-dependent reduction of target Pten mRNA in mouse hepatocytes by Pten ASOs modified with 2′ MOE (ION-116847), cEt (ION-582801), or 2′ F (ION-404130). ASOs were delivered by lipid transfection. The error bars represent standard deviation from three separate experiments. ( G ) Plasma levels of ALT and AST upon a single administration of PS-ASOs modified with either 2′ MOE or 2′ F at 400 mg/kg for 96 h. Seven pairs of 2′ MOE or 2′ F-modified sequences were included. Hepatotoxic potentials of the 2′ F PS-ASOs were rank-ordered and color-coded (red: high hepatotoxic potentials; and green: low hepatotoxic potentials) based on plasma levels of ALT and AST. N = 3 per group. F.D. = found death.

    Journal: Nucleic Acids Research

    Article Title: Acute hepatotoxicity of 2′ fluoro-modified 5–10–5 gapmer phosphorothioate oligonucleotides in mice correlates with intracellular protein binding and the loss of DBHS proteins

    doi: 10.1093/nar/gky060

    Figure Lengend Snippet: PS-ASOs induce hepatotoxicity in mice in a sugar modification-dependent manner. ( A ) Schematic representation of the 5–10–5 Pten gapmer ASO. ( B ) Plasma levels of ALT and AST upon a single administration of Pten PS-ASOs modified with 2′ MOE (ION-116847), cEt (ION-582801) or 2′ F (ION-404130) at 100 or 400 mg/kg for 96 h. N = 3 per group. ( C ) Plasma levels of ALT and AST upon a single administration of Pten LNA (ION-390896) at 100 or 400 mg/kg for 96 h. N = 3 per group. ( D ) Plasma levels of ALT and AST upon repeated administration of 2′ F ASO (ION-404130) at 20 mg/kg per dose, three doses per week. S-saline, and F-2′ F PS-ASO (ION-404130). ( E ) qRT-PCR of on-target Pten mRNA. Mice were treated as was described in B. The error bars indicate data acquired from three individual animals. ( F ) qRT-PCR showed dose-dependent reduction of target Pten mRNA in mouse hepatocytes by Pten ASOs modified with 2′ MOE (ION-116847), cEt (ION-582801), or 2′ F (ION-404130). ASOs were delivered by lipid transfection. The error bars represent standard deviation from three separate experiments. ( G ) Plasma levels of ALT and AST upon a single administration of PS-ASOs modified with either 2′ MOE or 2′ F at 400 mg/kg for 96 h. Seven pairs of 2′ MOE or 2′ F-modified sequences were included. Hepatotoxic potentials of the 2′ F PS-ASOs were rank-ordered and color-coded (red: high hepatotoxic potentials; and green: low hepatotoxic potentials) based on plasma levels of ALT and AST. N = 3 per group. F.D. = found death.

    Article Snippet: Blood samples were processed to plasma and evaluated for alanine aminotransferase (ALT) and aspartate aminotransferase (AST) using a Beckman Coulter AU480 Bioanalyzer.

    Techniques: Mouse Assay, Modification, Allele-specific Oligonucleotide, AST Assay, Quantitative RT-PCR, Transfection, Standard Deviation

    Time course of mRNA alterations and stress phenotypes induced by 2′ F PS-ASO (ION-404130) in vivo . ( A–F) Mice were treated with a single 400 mg/kg 2′ F PS-ASO (ION-404130) or with saline and indicated parameters were quantified at 24, 48, 72 and 96 h after dosing. N = 3 per group. The error bars indicate data acquired from three individual animals. S-saline, and F-2′ F PS-ASO (ION-404130). ( A ) qRT-PCR quantification of on-target Pten mRNA levels in liver. ( B ) Plasma levels of ALT and AST. ( C ) qRT-PCR of mRNA transcripts Cdkn1a/p21 and Gadd45a . ( D ) qRT-PCR of liver mRNA levels of anti-apoptosis markers Mcl1 and Bcl-xl . ( E ) qRT-PCR of liver mRNA levels of necrosis markers that are up-regulated ( Map3k6 and Cd68 ) or down-regulated ( Lgr5 and Rhbg ) by 2′ F ASO. ( F ) Representative images of H E staining and active caspase 3 staining of liver sections.

    Journal: Nucleic Acids Research

    Article Title: Acute hepatotoxicity of 2′ fluoro-modified 5–10–5 gapmer phosphorothioate oligonucleotides in mice correlates with intracellular protein binding and the loss of DBHS proteins

    doi: 10.1093/nar/gky060

    Figure Lengend Snippet: Time course of mRNA alterations and stress phenotypes induced by 2′ F PS-ASO (ION-404130) in vivo . ( A–F) Mice were treated with a single 400 mg/kg 2′ F PS-ASO (ION-404130) or with saline and indicated parameters were quantified at 24, 48, 72 and 96 h after dosing. N = 3 per group. The error bars indicate data acquired from three individual animals. S-saline, and F-2′ F PS-ASO (ION-404130). ( A ) qRT-PCR quantification of on-target Pten mRNA levels in liver. ( B ) Plasma levels of ALT and AST. ( C ) qRT-PCR of mRNA transcripts Cdkn1a/p21 and Gadd45a . ( D ) qRT-PCR of liver mRNA levels of anti-apoptosis markers Mcl1 and Bcl-xl . ( E ) qRT-PCR of liver mRNA levels of necrosis markers that are up-regulated ( Map3k6 and Cd68 ) or down-regulated ( Lgr5 and Rhbg ) by 2′ F ASO. ( F ) Representative images of H E staining and active caspase 3 staining of liver sections.

    Article Snippet: Blood samples were processed to plasma and evaluated for alanine aminotransferase (ALT) and aspartate aminotransferase (AST) using a Beckman Coulter AU480 Bioanalyzer.

    Techniques: Allele-specific Oligonucleotide, In Vivo, Mouse Assay, Quantitative RT-PCR, AST Assay, Staining

    PZH has no significant toxicity in EAE rats. (a) The organ coefficient of several organs from the respective group was detected. The levels of ALT (b) and AST (c) for hepatotoxicity as well as CREA (d) and UREA (e) for nephrotoxicity were shown, respectively.

    Journal: Journal of Immunology Research

    Article Title: Therapeutic Potential of Pien Tze Huang on Experimental Autoimmune Encephalomyelitis Rat

    doi: 10.1155/2018/2952471

    Figure Lengend Snippet: PZH has no significant toxicity in EAE rats. (a) The organ coefficient of several organs from the respective group was detected. The levels of ALT (b) and AST (c) for hepatotoxicity as well as CREA (d) and UREA (e) for nephrotoxicity were shown, respectively.

    Article Snippet: Blood Biochemical Determination For the detection of hematological biochemical parameters, alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine (CREA), and UREA nitrogen (UREA) were tested with blood biochemical commercial kits by a Japan's Hitachi 7160 automatic biochemical analyzer.

    Techniques: AST Assay

    Decoction of Hedyotis diffusa (HD) relieves the acute inflammation in hepatitis mouse. a Administration of HD decoction blocked LPS/GALN-induced up-regulation of mRNA levels of CXCL1, TNF-α, IL-1β, MIP-2. b The treatment of HD decoction reduced the expressed levels of TNF-α, IL-1β, IL-6 and MCP-1 in liver of LPS/GALN-injected mice. c Alanine transaminase (ALT) level was measured in control or LPS/GALN- and LPS/GALN + HD-treated mouse. HD decoction significantly reduced the level of ALT (n = 5). d Aspartate transaminase (AST) level was measured in control or LPS/GALN- and LPS/GALN + HD-treated mouse. HD decoction significantly reduced the level of AST (n = 5). e Survival of hepatitis mouse was measured after treatment of HD decoction (n = 20, per group) within 20 days. f Effect of HD on LPS/GALN-mediated liver histopathologic changes

    Journal: Chinese Medicine

    Article Title: Metabolic regulations of a decoction of Hedyotis diffusa in acute liver injury of mouse models

    doi: 10.1186/s13020-017-0159-4

    Figure Lengend Snippet: Decoction of Hedyotis diffusa (HD) relieves the acute inflammation in hepatitis mouse. a Administration of HD decoction blocked LPS/GALN-induced up-regulation of mRNA levels of CXCL1, TNF-α, IL-1β, MIP-2. b The treatment of HD decoction reduced the expressed levels of TNF-α, IL-1β, IL-6 and MCP-1 in liver of LPS/GALN-injected mice. c Alanine transaminase (ALT) level was measured in control or LPS/GALN- and LPS/GALN + HD-treated mouse. HD decoction significantly reduced the level of ALT (n = 5). d Aspartate transaminase (AST) level was measured in control or LPS/GALN- and LPS/GALN + HD-treated mouse. HD decoction significantly reduced the level of AST (n = 5). e Survival of hepatitis mouse was measured after treatment of HD decoction (n = 20, per group) within 20 days. f Effect of HD on LPS/GALN-mediated liver histopathologic changes

    Article Snippet: Measurement of serum alanine transaminase and aspartate transaminase activities Blood samples were centrifuged at 1500g for 20 min at 4 °C, and alanine transaminase (ALT) and aspartate transaminase (AST) activities in serum were measured by commercial kits from Randox Laboratories (UK).

    Techniques: Injection, Mouse Assay, AST Assay

    Effects of the FoxO1 inhibitor (AS) and APN on serum AST, ALT, and TG. Serum AST, ALT, and TG were determined by kits (Stanbio Laboratory, TX, USA). Values are expressed as mean ± SD, n = 6/group. ∗ P

    Journal: Journal of Diabetes Research

    Article Title: Enhancement of Adiponectin Ameliorates Nonalcoholic Fatty Liver Disease via Inhibition of FoxO1 in Type I Diabetic Rats

    doi: 10.1155/2018/6254340

    Figure Lengend Snippet: Effects of the FoxO1 inhibitor (AS) and APN on serum AST, ALT, and TG. Serum AST, ALT, and TG were determined by kits (Stanbio Laboratory, TX, USA). Values are expressed as mean ± SD, n = 6/group. ∗ P

    Article Snippet: Serum total aspartate aminotransferase (AST), alanine aminotransferase (ALT), and triglyceride (TG) were determined using commercially available kits (Stanbio Laboratory, TX, USA), respectively.

    Techniques: AST Assay