agilent 2100 bioanalyzer Search Results


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  • 99
    Agilent technologies agilent 2100 bioanalyzer
    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using <t>Agilent</t> 2100 <t>Bioanalyzer</t> with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
    Agilent 2100 Bioanalyzer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 107404 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher agilent 2100 bioanalyzer
    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using <t>Agilent</t> 2100 <t>Bioanalyzer</t> with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
    Agilent 2100 Bioanalyzer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 376 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Shanghai Biochip Co. Ltd agilent bioanalyzer 2100
    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using <t>Agilent</t> 2100 <t>Bioanalyzer</t> with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
    Agilent Bioanalyzer 2100, supplied by Shanghai Biochip Co. Ltd, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Illumina Inc agilent 2100 bioanalyzer
    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using <t>Agilent</t> 2100 <t>Bioanalyzer</t> with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
    Agilent 2100 Bioanalyzer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 97/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Stratagene agilent 2100 bioanalyzer
    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using <t>Agilent</t> 2100 <t>Bioanalyzer</t> with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
    Agilent 2100 Bioanalyzer, supplied by Stratagene, used in various techniques. Bioz Stars score: 87/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Bio-Rad agilent 2100 bioanalyzer
    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using <t>Agilent</t> 2100 <t>Bioanalyzer</t> with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
    Agilent 2100 Bioanalyzer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Biogen Inc agilent 2100 bioanalyzer
    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using <t>Agilent</t> 2100 <t>Bioanalyzer</t> with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
    Agilent 2100 Bioanalyzer, supplied by Biogen Inc, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Hewlett-Packard agilent bioanalyzer 2100
    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using <t>Agilent</t> 2100 <t>Bioanalyzer</t> with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
    Agilent Bioanalyzer 2100, supplied by Hewlett-Packard, used in various techniques. Bioz Stars score: 87/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Agilent technologies bioanalyzer agilant 2100
    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using <t>Agilent</t> 2100 <t>Bioanalyzer</t> with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
    Bioanalyzer Agilant 2100, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Roche agilent bioanalyzer 2100
    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using <t>Agilent</t> 2100 <t>Bioanalyzer</t> with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
    Agilent Bioanalyzer 2100, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Santa Cruz Biotechnology agilent bioanalyzer 2100
    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using <t>Agilent</t> 2100 <t>Bioanalyzer</t> with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
    Agilent Bioanalyzer 2100, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    CD Genomics agilent 2100 bioanalyzer
    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using <t>Agilent</t> 2100 <t>Bioanalyzer</t> with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
    Agilent 2100 Bioanalyzer, supplied by CD Genomics, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Caliper Life Sciences agilent 2100 bioanalyzer
    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using <t>Agilent</t> 2100 <t>Bioanalyzer</t> with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
    Agilent 2100 Bioanalyzer, supplied by Caliper Life Sciences, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    GATC Biotech agilent 2100 bioanalyzer
    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using <t>Agilent</t> 2100 <t>Bioanalyzer</t> with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
    Agilent 2100 Bioanalyzer, supplied by GATC Biotech, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Agilent technologies agilent 2100 bioanalyzer system
    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using <t>Agilent</t> 2100 <t>Bioanalyzer</t> with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
    Agilent 2100 Bioanalyzer System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Matriks AS agilent 2100 bioanalyzer
    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using <t>Agilent</t> 2100 <t>Bioanalyzer</t> with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
    Agilent 2100 Bioanalyzer, supplied by Matriks AS, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Beckman Coulter agilent 2100 bioanalyzer
    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using <t>Agilent</t> 2100 <t>Bioanalyzer</t> with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
    Agilent 2100 Bioanalyzer, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Promega agilent 2100 bioanalyzer
    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using <t>Agilent</t> 2100 <t>Bioanalyzer</t> with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
    Agilent 2100 Bioanalyzer, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    GE Healthcare agilent 2100 bioanalyzer
    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using <t>Agilent</t> 2100 <t>Bioanalyzer</t> with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
    Agilent 2100 Bioanalyzer, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 89/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Genewiz agilent 2100 bioanalyzer
    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using <t>Agilent</t> 2100 <t>Bioanalyzer</t> with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
    Agilent 2100 Bioanalyzer, supplied by Genewiz, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Agilent technologies agilent bioanlalyzer 2100
    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using <t>Agilent</t> 2100 <t>Bioanalyzer</t> with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
    Agilent Bioanlalyzer 2100, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Agilent technologies agilent 2100 bioanlayzer
    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using <t>Agilent</t> 2100 <t>Bioanalyzer</t> with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
    Agilent 2100 Bioanlayzer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Agilent technologies agilent 2100 bioanalyzier
    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using <t>Agilent</t> 2100 <t>Bioanalyzer</t> with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
    Agilent 2100 Bioanalyzier, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 95/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Agilent technologies agilant 2100 bioanalyser
    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using <t>Agilent</t> 2100 <t>Bioanalyzer</t> with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
    Agilant 2100 Bioanalyser, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Thermo Fisher agilent 2100 bioanalyser
    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using <t>Agilent</t> 2100 <t>Bioanalyzer</t> with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
    Agilent 2100 Bioanalyser, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Agilent technologies agilent 2100 bioanalyazer
    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using <t>Agilent</t> 2100 <t>Bioanalyzer</t> with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
    Agilent 2100 Bioanalyazer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Agilent technologies agilent 2100 bioanalayzer
    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using <t>Agilent</t> 2100 <t>Bioanalyzer</t> with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
    Agilent 2100 Bioanalayzer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Agilent technologies agilent technologies 2100 bioanalyze
    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using <t>Agilent</t> 2100 <t>Bioanalyzer</t> with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
    Agilent Technologies 2100 Bioanalyze, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using Agilent 2100 Bioanalyzer with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.

    Journal: BMC Research Notes

    Article Title: Ensuring good quality rna for quantitative real-time pcr isolated from renal proximal tubular cells using laser capture microdissection

    doi: 10.1186/1756-0500-7-62

    Figure Lengend Snippet: Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using Agilent 2100 Bioanalyzer with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.

    Article Snippet: RNA integrity number (RIN) RIN was measured using Agilent 2100 Bioanalyzer with Agilent RNA 6000 Pico Kit.

    Techniques: Concentration Assay, Produced

    Electropherogram and “gel-like” image of positive control on the Agilent Bioanalyzer 2100. The x -axis on the electropherogram represents amplicon size (bp), whist the y -axis represents the measurement response of fluorescence units (FUs). Amplicons of interest in order of size (bp) are lower marker (15), Zein (80), Lectin (90), CaMV (108) and combined CaMV 35s promoter/NOS terminator (131) amplicons, and the upper marker (1500).

    Journal: Biotechnology Research International

    Article Title: Applicability of Three Alternative Instruments for Food Authenticity Analysis: GMO Identification

    doi: 10.4061/2011/838232

    Figure Lengend Snippet: Electropherogram and “gel-like” image of positive control on the Agilent Bioanalyzer 2100. The x -axis on the electropherogram represents amplicon size (bp), whist the y -axis represents the measurement response of fluorescence units (FUs). Amplicons of interest in order of size (bp) are lower marker (15), Zein (80), Lectin (90), CaMV (108) and combined CaMV 35s promoter/NOS terminator (131) amplicons, and the upper marker (1500).

    Article Snippet: Many DNA detection and screening protocols that utilise end-point PCR and have been applied on the Agilent Bioanalyzer 2100 have been validated and endorsed by the UK Food Standards Agency (FSA).

    Techniques: Positive Control, Amplification, Fluorescence, Marker

    Size distribution of mRNA, cRNA and dsDNA on Agilent 2100 Bioanalyzer 6000 Nanochips. ( a ) Universal Human Reference (UHR) RNA and sense-RNA template library after IVT-amplification. Lane L displays the ladder (25, 200, 500, 1000, 2000 and 4000 nt). Lane 1 contains fresh UHR RNA. Lanes 2, 3 and 4 display three individual IVT-amplifications of sense-RNA using total RNA displayed in lane 1. ( b ) Size distribution of fresh, frozen, FFPE-RNA and amplified cRNA. Lane L displays the same ladder as observed in (a). Lane 1 contains fresh human breast RNA. Lane 2 contains total RNA from the 10-year-old frozen human breast cancer tissue. Lane 3 contains total RNA from the matched 10-year-old FFPE human breast cancer tissue. Lane 4 contains cRNA obtained by IVT-amplifications of 10-year-old frozen RNA (lane 2). Lanes 5 contains cRNA obtained by direct IVT-amplification of the 10-year-old FFPE-RNA (lane 3). Lanes 6 contains amplified cRNA obtained by CT-RT and IVT-amplification of the same 10-year-old FFPE-RNA. ( c ) Size distribution of double-stranded DNA on a Bioanalyzer 2100 Agilent nanochip. Lane L displays the ladder. Lane 1 displays dsDNA obtained from 10-year-old frozen RNA. Lane 2 displays dsDNA obtained from 10-year-old FFPE-RNA. Lane 3 shows dsDNA obtained after CT-RT and double-strand DNA synthesis of the same 10-year-old FFPE-RNA. (See Supplementary Data for fragmented RNA profiles)

    Journal: Nucleic Acids Research

    Article Title: Molecular restoration of archived transcriptional profiles by complementary-template reverse-transcription (CT-RT)

    doi: 10.1093/nar/gkm510

    Figure Lengend Snippet: Size distribution of mRNA, cRNA and dsDNA on Agilent 2100 Bioanalyzer 6000 Nanochips. ( a ) Universal Human Reference (UHR) RNA and sense-RNA template library after IVT-amplification. Lane L displays the ladder (25, 200, 500, 1000, 2000 and 4000 nt). Lane 1 contains fresh UHR RNA. Lanes 2, 3 and 4 display three individual IVT-amplifications of sense-RNA using total RNA displayed in lane 1. ( b ) Size distribution of fresh, frozen, FFPE-RNA and amplified cRNA. Lane L displays the same ladder as observed in (a). Lane 1 contains fresh human breast RNA. Lane 2 contains total RNA from the 10-year-old frozen human breast cancer tissue. Lane 3 contains total RNA from the matched 10-year-old FFPE human breast cancer tissue. Lane 4 contains cRNA obtained by IVT-amplifications of 10-year-old frozen RNA (lane 2). Lanes 5 contains cRNA obtained by direct IVT-amplification of the 10-year-old FFPE-RNA (lane 3). Lanes 6 contains amplified cRNA obtained by CT-RT and IVT-amplification of the same 10-year-old FFPE-RNA. ( c ) Size distribution of double-stranded DNA on a Bioanalyzer 2100 Agilent nanochip. Lane L displays the ladder. Lane 1 displays dsDNA obtained from 10-year-old frozen RNA. Lane 2 displays dsDNA obtained from 10-year-old FFPE-RNA. Lane 3 shows dsDNA obtained after CT-RT and double-strand DNA synthesis of the same 10-year-old FFPE-RNA. (See Supplementary Data for fragmented RNA profiles)

    Article Snippet: The CT-RT process provides access to larger cDNA and cRNA transcripts In order to investigate the benefit of the CT-RT process over the single RT of short FFPE-RNA, we compared the size distribution of cRNA and cDNA products for each of our T7 IVT-amplifications on a bioanalyzer 2100 Agilent.

    Techniques: Amplification, Formalin-fixed Paraffin-Embedded, DNA Synthesis

    FANCI functions in LSU pre-rRNA processing. ( A ). The 41S, 32S, and 12S pre-rRNAs are detected by a probe (P5) that hybridizes with ITS2. ( B ) FANCI depletion results in LSU pre-rRNA processing defects. HeLa cells were transfected with the indicated siRNAs, and total RNA was harvested after 72 h of depletion. Pre-rRNAs were separated by gel electrophoresis, and Northern blots were performed using radioactively labeled P5 probe. Northern blotting with a probe against the 7SL RNA was used as a loading control. Small illustrations of the pre-rRNAs are to the right of their respective bands. ( C – I ) profiles for Northern blotting from three biological replicates for mock ( C ), siFANCI-pool ( D ), siFANCI-2 ( E ), siFANCI-3 ( F ), siPES1 ( G ), siNOL11 ( H ), and siFANCD2 ( I ). Statistical significance was calculated using a two-way ANOVA with Sidak multiple comparisons test (mean ± SD). All comparisons are relative to siNT. The x axes of all graphs were equalized, except for siPES1 in G , which has larger RAMP values. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. Unmarked bars are not significant. ( J ) FANCI depletion results in decreased mature 28S rRNA relative to 18S. The ratio of 28S/18S was calculated using an Agilent 2100 Bioanalyzer. Statistical significance was calculated using a one-tailed unpaired Mann–Whitney U test for three biological replicates (mean ± SD). All comparisons are relative to siNT.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Fanconi anemia protein FANCI functions in ribosome biogenesis

    doi: 10.1073/pnas.1811557116

    Figure Lengend Snippet: FANCI functions in LSU pre-rRNA processing. ( A ). The 41S, 32S, and 12S pre-rRNAs are detected by a probe (P5) that hybridizes with ITS2. ( B ) FANCI depletion results in LSU pre-rRNA processing defects. HeLa cells were transfected with the indicated siRNAs, and total RNA was harvested after 72 h of depletion. Pre-rRNAs were separated by gel electrophoresis, and Northern blots were performed using radioactively labeled P5 probe. Northern blotting with a probe against the 7SL RNA was used as a loading control. Small illustrations of the pre-rRNAs are to the right of their respective bands. ( C – I ) profiles for Northern blotting from three biological replicates for mock ( C ), siFANCI-pool ( D ), siFANCI-2 ( E ), siFANCI-3 ( F ), siPES1 ( G ), siNOL11 ( H ), and siFANCD2 ( I ). Statistical significance was calculated using a two-way ANOVA with Sidak multiple comparisons test (mean ± SD). All comparisons are relative to siNT. The x axes of all graphs were equalized, except for siPES1 in G , which has larger RAMP values. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. Unmarked bars are not significant. ( J ) FANCI depletion results in decreased mature 28S rRNA relative to 18S. The ratio of 28S/18S was calculated using an Agilent 2100 Bioanalyzer. Statistical significance was calculated using a one-tailed unpaired Mann–Whitney U test for three biological replicates (mean ± SD). All comparisons are relative to siNT.

    Article Snippet: To measure the ratio of mature 28S to 18S rRNAs, total RNA that was prepared as described above was run on an Agilent Technologies 2100 Bioanalyzer at the Yale Center for Genome Analysis.

    Techniques: Transfection, Nucleic Acid Electrophoresis, Northern Blot, Labeling, One-tailed Test, MANN-WHITNEY

    Absence of gnd gene in S. bongori strains . The gnd gene was PCR-amplified as described in the Materials and Methods from eight different strains of S. bongori . PCR products were resolved using the Agilent 7500 DNA kit and the Agilent 2100 Bioanalyzer. Negative Control 1 corresponds to PCR reagents mix + water as a template; and Negative Control 2 corresponds to DNA from a known gnd negative bacterial strain using our PCR conditions.

    Journal: Frontiers in Microbiology

    Article Title: Differentiation of Salmonella strains from the SARA, SARB and SARC reference collections by using three genes PCR-RFLP and the 2100 Agilent Bioanalyzer

    doi: 10.3389/fmicb.2014.00417

    Figure Lengend Snippet: Absence of gnd gene in S. bongori strains . The gnd gene was PCR-amplified as described in the Materials and Methods from eight different strains of S. bongori . PCR products were resolved using the Agilent 7500 DNA kit and the Agilent 2100 Bioanalyzer. Negative Control 1 corresponds to PCR reagents mix + water as a template; and Negative Control 2 corresponds to DNA from a known gnd negative bacterial strain using our PCR conditions.

    Article Snippet: Previous studies have demonstrated improved accuracy and reproducibility of RFLP using the 2100 Agilent Bioanalyzer for the sizing of the DNA fragments (Panaro et al., ; Nachamkin et al., ; Lu et al., ; Hathaway et al., ).

    Techniques: Polymerase Chain Reaction, Amplification, Negative Control

    A representation of the gel image generated by the Agilent 2100 Bioanalyzer for multiplex PCR Serotyping method of Salmonella enterica serovar Typhimurium in Horse Feed (HF), Whole Oats (OT), Dried Beet Pulp (DBP), Calf Milk Replacer (CMR), Dried Molasses (DM) and Wheat Brand (WB) spiked with 10 CFU/25 g, 50 CFU/25 g and 100 CFU/25 g. Fig. 1 a–f (Lane 1) Ladder, (Lane 2–4) 100 CFU/25 g of reaction 1–3, (Lane 5–7) 50 CFU/25 g of reaction 1–3, (Lane 8–10) 10 CFU/25 g of reaction 1–3. Figure a is for HF, Figure b is for OT, Figure c is for DM, Figure d is for WB, Figure e is for CMR, and Figure f is for DBP.

    Journal: MethodsX

    Article Title: Evaluation of a multiplex PCR method to serotype Salmonella in animal feeds pre-enrichment broth cultures

    doi: 10.1016/j.mex.2017.09.003

    Figure Lengend Snippet: A representation of the gel image generated by the Agilent 2100 Bioanalyzer for multiplex PCR Serotyping method of Salmonella enterica serovar Typhimurium in Horse Feed (HF), Whole Oats (OT), Dried Beet Pulp (DBP), Calf Milk Replacer (CMR), Dried Molasses (DM) and Wheat Brand (WB) spiked with 10 CFU/25 g, 50 CFU/25 g and 100 CFU/25 g. Fig. 1 a–f (Lane 1) Ladder, (Lane 2–4) 100 CFU/25 g of reaction 1–3, (Lane 5–7) 50 CFU/25 g of reaction 1–3, (Lane 8–10) 10 CFU/25 g of reaction 1–3. Figure a is for HF, Figure b is for OT, Figure c is for DM, Figure d is for WB, Figure e is for CMR, and Figure f is for DBP.

    Article Snippet: Major equipment and supplies for PCR assay • Vitek® 2 Compact, Version 5, (Biomerieux, St Louis, MO) • Roche MagNA Pure Compact (Roche, Indianapolis IN) • BioRad conventional C1000 thermocycler (BioRad, Hercules, CA) • Eppendorf Centrifuge 5424R (Eppendorf, Hauppauge, NY) Agilent 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany) • DNA 1000 Reagents kit for DNA analysis (Agilent Technologies, Waldbronn, Germany) • PCR tubes (BioRad, Hercules, CA)) • Sterile Eppendorf style microcentrifuge tubes ((Life Sciences, Hercules, CA or equivalent) • Sterile inoculating loops or needles (Life Sciences, Hercules, CA or equivalent) • Ice bucket or bench top cooler • Adjustable Micropipettors (0.1–1000 μl) • Aerosol resistant micropipettor tips (0.1–1000 μl) • Vortex Mixer (Life Sciences, or equivalent) • Microcentrifuge tips (Life Sciences, Hercules, CA or equivalent)

    Techniques: Generated, Multiplex Assay, Polymerase Chain Reaction, Western Blot

    Serotyping reaction 1, and 2 demonstrated in the Agilent 2100 Bioanalyzer gel image for the various Salmonella enterica serovars: (Lane 1) Ladder; (Lane 2–3) Typhimurium from Horse Feed with serotypic banding pattern ABCDEI; (Lane 4–5) Agona for Whole Oats with serotypic banding pattern BCJ; (Lane 6–7) Hadar for Calf Milk Replacer with serotypic banding pattern BC.

    Journal: MethodsX

    Article Title: Evaluation of a multiplex PCR method to serotype Salmonella in animal feeds pre-enrichment broth cultures

    doi: 10.1016/j.mex.2017.09.003

    Figure Lengend Snippet: Serotyping reaction 1, and 2 demonstrated in the Agilent 2100 Bioanalyzer gel image for the various Salmonella enterica serovars: (Lane 1) Ladder; (Lane 2–3) Typhimurium from Horse Feed with serotypic banding pattern ABCDEI; (Lane 4–5) Agona for Whole Oats with serotypic banding pattern BCJ; (Lane 6–7) Hadar for Calf Milk Replacer with serotypic banding pattern BC.

    Article Snippet: Major equipment and supplies for PCR assay • Vitek® 2 Compact, Version 5, (Biomerieux, St Louis, MO) • Roche MagNA Pure Compact (Roche, Indianapolis IN) • BioRad conventional C1000 thermocycler (BioRad, Hercules, CA) • Eppendorf Centrifuge 5424R (Eppendorf, Hauppauge, NY) Agilent 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany) • DNA 1000 Reagents kit for DNA analysis (Agilent Technologies, Waldbronn, Germany) • PCR tubes (BioRad, Hercules, CA)) • Sterile Eppendorf style microcentrifuge tubes ((Life Sciences, Hercules, CA or equivalent) • Sterile inoculating loops or needles (Life Sciences, Hercules, CA or equivalent) • Ice bucket or bench top cooler • Adjustable Micropipettors (0.1–1000 μl) • Aerosol resistant micropipettor tips (0.1–1000 μl) • Vortex Mixer (Life Sciences, or equivalent) • Microcentrifuge tips (Life Sciences, Hercules, CA or equivalent)

    Techniques:

    Isolation of cotton tissues from paraffin-embedded sections by laser microdissection (LMD). Isolation of cotton tissues from paraffin-embedded sections by laser microdissection (LMD). Sections before LMD (a, c, e) , and sections after LM (b, d, f) . The area selected for laser microdissection is outlined in green (a region near the damage caused by larvae feeding, which comprised the stamen tissue, viewed at a , c and d ) or blue (a region farther from the injured area, which comprised the carpel tissue, viewed at a , e , and f ). The assessment of extracted RNA integrity from the stamen and carpel are shown in g and h , respectively. Electropherograms were obtained with an Agilent 2100 Bioanalyser. Open and closed arrowheads indicate the 18S and 28S ribosomal RNA peaks, respectively. RNA quality is expressed as the RNA integrity number (RIN). Scale bars = 100 μm.

    Journal: BMC Genomics

    Article Title: Transcriptome analysis of Gossypium hirsutum flower buds infested by cotton boll weevil (Anthonomus grandis) larvae

    doi: 10.1186/1471-2164-15-854

    Figure Lengend Snippet: Isolation of cotton tissues from paraffin-embedded sections by laser microdissection (LMD). Isolation of cotton tissues from paraffin-embedded sections by laser microdissection (LMD). Sections before LMD (a, c, e) , and sections after LM (b, d, f) . The area selected for laser microdissection is outlined in green (a region near the damage caused by larvae feeding, which comprised the stamen tissue, viewed at a , c and d ) or blue (a region farther from the injured area, which comprised the carpel tissue, viewed at a , e , and f ). The assessment of extracted RNA integrity from the stamen and carpel are shown in g and h , respectively. Electropherograms were obtained with an Agilent 2100 Bioanalyser. Open and closed arrowheads indicate the 18S and 28S ribosomal RNA peaks, respectively. RNA quality is expressed as the RNA integrity number (RIN). Scale bars = 100 μm.

    Article Snippet: The quality of total RNA extracted from LMD-collected tissues was assessed using an RNA 6000 Pico kit on the Agilent 2100 Bioanalyser (Agilent Technologies).

    Techniques: Isolation, Laser Capture Microdissection

    Agilent BioAnalyser 2100 traces of total RNA samples. Different volumes of peripheral blood were processed using PAXgene reagent as described in the text. A) Full scale 2.5 mL peripheral blood extraction B) 1.0 mL peripheral blood scaled down extraction C) 0.3 mL peripheral blood scaled down extraction D) Stratagene Universal RNA. The 2.5 mL and 1.0 mL extractions were run on eukaryote total RNA Nano chips and the 0.3 mL extractions and the Universal RNA shown were run on Pico chips. The 18s and 28s RNA peaks can be seen at approximately 42 and 48 seconds respectively.

    Journal: BMC Immunology

    Article Title: Successful downstream application of the Paxgene Blood RNA system from small blood samples in paediatric patients for quantitative PCR analysis

    doi: 10.1186/1471-2172-8-20

    Figure Lengend Snippet: Agilent BioAnalyser 2100 traces of total RNA samples. Different volumes of peripheral blood were processed using PAXgene reagent as described in the text. A) Full scale 2.5 mL peripheral blood extraction B) 1.0 mL peripheral blood scaled down extraction C) 0.3 mL peripheral blood scaled down extraction D) Stratagene Universal RNA. The 2.5 mL and 1.0 mL extractions were run on eukaryote total RNA Nano chips and the 0.3 mL extractions and the Universal RNA shown were run on Pico chips. The 18s and 28s RNA peaks can be seen at approximately 42 and 48 seconds respectively.

    Article Snippet: RNA quality was assessed using the Agilent BioAnalyser 2100 and an in-house TaqMan™ assay which measures GAPDH transcript integrity by determining 3' to 5' ratios. qPCR analysis was performed on an additional panel of 7 housekeeping genes.

    Techniques:

    2100 high sensitivity DNA Bioanalyzer S . aureus . Bacteria were purged for 15 minutes and then lysed with Lyse-It for 30 seconds at 30% power (A and B) and 60 seconds at 50% power (C and D) . A and C) Total concentrations (pg/μL) below the reported base pairs. B and D) Concentrations (pg/μL) between base pair ranges.

    Journal: PLoS ONE

    Article Title: Elucidation of a non-thermal mechanism for DNA/RNA fragmentation and protein degradation when using Lyse-It

    doi: 10.1371/journal.pone.0225475

    Figure Lengend Snippet: 2100 high sensitivity DNA Bioanalyzer S . aureus . Bacteria were purged for 15 minutes and then lysed with Lyse-It for 30 seconds at 30% power (A and B) and 60 seconds at 50% power (C and D) . A and C) Total concentrations (pg/μL) below the reported base pairs. B and D) Concentrations (pg/μL) between base pair ranges.

    Article Snippet: For the 2100 Agilent Bioanalyzer preparation, all samples were diluted down by 50% (10 μL sample to 10 μL DI water).

    Techniques:

    2100 high sensitivity DNA Bioanalyzer of V . cholerae . Bacteria were purged for 15 minutes and then lysed with Lyse-It for 30 seconds at 30% power. ( A and B) and 50% power, 60 seconds (C and D) Total concentrations (pg/μL) below the reported base pairs post 30% power, 30 seconds ( A) Concentrations (pg/μL) between base pair ranges for 30% power 30 seconds, (B) Total concentration before the reported base pairs post 50% power, 60 seconds (C) , and concentration between base pair ranges for 50% power, 60 seconds (D) .

    Journal: PLoS ONE

    Article Title: Elucidation of a non-thermal mechanism for DNA/RNA fragmentation and protein degradation when using Lyse-It

    doi: 10.1371/journal.pone.0225475

    Figure Lengend Snippet: 2100 high sensitivity DNA Bioanalyzer of V . cholerae . Bacteria were purged for 15 minutes and then lysed with Lyse-It for 30 seconds at 30% power. ( A and B) and 50% power, 60 seconds (C and D) Total concentrations (pg/μL) below the reported base pairs post 30% power, 30 seconds ( A) Concentrations (pg/μL) between base pair ranges for 30% power 30 seconds, (B) Total concentration before the reported base pairs post 50% power, 60 seconds (C) , and concentration between base pair ranges for 50% power, 60 seconds (D) .

    Article Snippet: For the 2100 Agilent Bioanalyzer preparation, all samples were diluted down by 50% (10 μL sample to 10 μL DI water).

    Techniques: Concentration Assay

    Microfluidic electrophoresis of HTP96-extracted RNA . The quality of HTP96 RNA (4 μL at 300 ng μL -1 ) was measured on an Agilent 2100 Bioanalyzer microfluidic electrophoresis chip following treatment with DNase. The microfluidic electrophoresis image (inset) and electropherogram are typical of high quality Arabidopsis RNA showing the clear cytosolic and plastidic (Cp, asterisks) ribosomal bands. RNA species of low molecular weight are also apparent. gDNA contamination is effectively removed by DNase treatment.

    Journal: Plant Methods

    Article Title: Protocol: A simple phenol-based method for 96-well extraction of high quality RNA from Arabidopsis

    doi: 10.1186/1746-4811-7-7

    Figure Lengend Snippet: Microfluidic electrophoresis of HTP96-extracted RNA . The quality of HTP96 RNA (4 μL at 300 ng μL -1 ) was measured on an Agilent 2100 Bioanalyzer microfluidic electrophoresis chip following treatment with DNase. The microfluidic electrophoresis image (inset) and electropherogram are typical of high quality Arabidopsis RNA showing the clear cytosolic and plastidic (Cp, asterisks) ribosomal bands. RNA species of low molecular weight are also apparent. gDNA contamination is effectively removed by DNase treatment.

    Article Snippet: • (Optional) Agilent 2100 Bioanalyzer (Agilent Technologies).

    Techniques: Electrophoresis, Chromatin Immunoprecipitation, Molecular Weight