agei Takara Search Results


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  • 95
    New England Biolabs agei
    Agei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 793 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agei/product/New England Biolabs
    Average 95 stars, based on 793 article reviews
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    agei - by Bioz Stars, 2020-02
    95/100 stars
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    75
    TaKaRa xhoi agei digested pecfp c1
    Xhoi Agei Digested Pecfp C1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 75 stars, based on 1 article reviews
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    xhoi agei digested pecfp c1 - by Bioz Stars, 2020-02
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    84
    TaKaRa agei restriction sites
    Agei Restriction Sites, supplied by TaKaRa, used in various techniques. Bioz Stars score: 84/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 84 stars, based on 74 article reviews
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    agei restriction sites - by Bioz Stars, 2020-02
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    78
    TaKaRa ecori agei
    Ecori Agei, supplied by TaKaRa, used in various techniques. Bioz Stars score: 78/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 78 stars, based on 9 article reviews
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    ecori agei - by Bioz Stars, 2020-02
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    87
    TaKaRa dsred2 coding region
    Dsred2 Coding Region, supplied by TaKaRa, used in various techniques. Bioz Stars score: 87/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 87 stars, based on 21 article reviews
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    dsred2 coding region - by Bioz Stars, 2020-02
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    78
    TaKaRa ecori agei digested pegfp n1 vector
    Ecori Agei Digested Pegfp N1 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 78/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 78 stars, based on 8 article reviews
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    ecori agei digested pegfp n1 vector - by Bioz Stars, 2020-02
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    90
    TaKaRa egfp tag
    Egfp Tag, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    egfp tag - by Bioz Stars, 2020-02
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    79
    TaKaRa asei agei digested pegfp n1 plasmid
    Asei Agei Digested Pegfp N1 Plasmid, supplied by TaKaRa, used in various techniques. Bioz Stars score: 79/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    TaKaRa in fusion hd cloning kit
    In Fusion Hd Cloning Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 12308 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 12308 article reviews
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    in fusion hd cloning kit - by Bioz Stars, 2020-02
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    78
    TaKaRa ecori agei cleaved pegfp n1 plasmid
    Ecori Agei Cleaved Pegfp N1 Plasmid, supplied by TaKaRa, used in various techniques. Bioz Stars score: 78/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa pegfp c1
    Pegfp C1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 880 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 880 article reviews
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    pegfp c1 - by Bioz Stars, 2020-02
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    99
    TaKaRa peyfp c1
    Peyfp C1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1998 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 1998 article reviews
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    peyfp c1 - by Bioz Stars, 2020-02
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    99
    TaKaRa pgbkt7 vectors
    Pgbkt7 Vectors, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 346 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 346 article reviews
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    pgbkt7 vectors - by Bioz Stars, 2020-02
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    99
    TaKaRa pegfp n1
    Single-Cell Electroporation Can Be Carried Out at High Efficiency by Front-Loading Micropipettes. (a) In a micropipette filled with saline (scale bar 1 mm), a front-loaded reagent diffuses over time, decreasing concentration of the sample at the tip (drawings are not to scale). (b) Approximately 2 nL of three different fluorescent molecules, Alexa Fluor 594 (Dye1) and 488 (Dye2) hydrazide salts and SYBR-Green-labeled <t>pEGFP-N1</t> (Plasmid), were front-loaded into micropipettes and their fluorescence monitored at 1 minute intervals over ten minutes and compared to simulations (continuous lines). Each data point is the mean ± s.d. of three independent experiments. (c) Concentration of plasmid in the tip of the micropipette remains stable enough to reliably transfect multiple cells with pEGFP-N1. 79.1±8.7% of the transfected cells expressed EGFP 24 hours following transfection ( n = 72 from six independent experiments, where 12 cells were transfected within 2 minutes in each experiment). Scale bar 50 µm.
    Pegfp N1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 14774 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pegfp n1 - by Bioz Stars, 2020-02
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    99
    TaKaRa pegfp c3
    Single-Cell Electroporation Can Be Carried Out at High Efficiency by Front-Loading Micropipettes. (a) In a micropipette filled with saline (scale bar 1 mm), a front-loaded reagent diffuses over time, decreasing concentration of the sample at the tip (drawings are not to scale). (b) Approximately 2 nL of three different fluorescent molecules, Alexa Fluor 594 (Dye1) and 488 (Dye2) hydrazide salts and SYBR-Green-labeled <t>pEGFP-N1</t> (Plasmid), were front-loaded into micropipettes and their fluorescence monitored at 1 minute intervals over ten minutes and compared to simulations (continuous lines). Each data point is the mean ± s.d. of three independent experiments. (c) Concentration of plasmid in the tip of the micropipette remains stable enough to reliably transfect multiple cells with pEGFP-N1. 79.1±8.7% of the transfected cells expressed EGFP 24 hours following transfection ( n = 72 from six independent experiments, where 12 cells were transfected within 2 minutes in each experiment). Scale bar 50 µm.
    Pegfp C3, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 187 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pegfp c3 - by Bioz Stars, 2020-02
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    79
    TaKaRa pyfp n1
    Single-Cell Electroporation Can Be Carried Out at High Efficiency by Front-Loading Micropipettes. (a) In a micropipette filled with saline (scale bar 1 mm), a front-loaded reagent diffuses over time, decreasing concentration of the sample at the tip (drawings are not to scale). (b) Approximately 2 nL of three different fluorescent molecules, Alexa Fluor 594 (Dye1) and 488 (Dye2) hydrazide salts and SYBR-Green-labeled <t>pEGFP-N1</t> (Plasmid), were front-loaded into micropipettes and their fluorescence monitored at 1 minute intervals over ten minutes and compared to simulations (continuous lines). Each data point is the mean ± s.d. of three independent experiments. (c) Concentration of plasmid in the tip of the micropipette remains stable enough to reliably transfect multiple cells with pEGFP-N1. 79.1±8.7% of the transfected cells expressed EGFP 24 hours following transfection ( n = 72 from six independent experiments, where 12 cells were transfected within 2 minutes in each experiment). Scale bar 50 µm.
    Pyfp N1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 79/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pyfp n1 - by Bioz Stars, 2020-02
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    99
    TaKaRa pecfp n1
    Single-Cell Electroporation Can Be Carried Out at High Efficiency by Front-Loading Micropipettes. (a) In a micropipette filled with saline (scale bar 1 mm), a front-loaded reagent diffuses over time, decreasing concentration of the sample at the tip (drawings are not to scale). (b) Approximately 2 nL of three different fluorescent molecules, Alexa Fluor 594 (Dye1) and 488 (Dye2) hydrazide salts and SYBR-Green-labeled <t>pEGFP-N1</t> (Plasmid), were front-loaded into micropipettes and their fluorescence monitored at 1 minute intervals over ten minutes and compared to simulations (continuous lines). Each data point is the mean ± s.d. of three independent experiments. (c) Concentration of plasmid in the tip of the micropipette remains stable enough to reliably transfect multiple cells with pEGFP-N1. 79.1±8.7% of the transfected cells expressed EGFP 24 hours following transfection ( n = 72 from six independent experiments, where 12 cells were transfected within 2 minutes in each experiment). Scale bar 50 µm.
    Pecfp N1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1479 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pecfp n1 - by Bioz Stars, 2020-02
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    99
    TaKaRa pqcxip
    Increased RNA encapsidation in virions of the N17K mutant. (A) For quantification of the vector and helper RNAs packaged in virions of the NC N17K mutant, 293T cells were transfected with <t>pHR′-CMV-GFP,</t> pMD.G, and pHIVgptSVPA expressing the N17K mutant or wild-type Gag. At 2 days posttransfection, RNAs were extracted from virions purified through a 25% sucrose cushion and analyzed by slot blot hybridization with either a GFP (top panel) or a GPT (bottom panel) riboprobe to detect vector and helper RNAs, respectively. To compare the encapsidation of foreign RNAs by wild-type and N17K virions, we transfected 293T cells with <t>pQCXIP-gfp-C1</t> and pHIVgptSVPA expressing the N17K mutant or wild-type Gag. At 2 days posttransfection, total RNAs were extracted from the cells and from virions normalized by exogenous RT. The RNAs were reverse transcribed and the indicated dilutions of cDNA were amplified by PCR with primers specific to GFP. This semiquantitative analysis was performed with virion (B) and cellular (C) samples. Undiluted samples of virion and cellular RNA prior to reverse transcription (no RT) were used to verify the absence of contaminating plasmid DNA (D). PCR samples were electrophoresed in a 2% agarose gel.
    Pqcxip, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 651 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 651 article reviews
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    pqcxip - by Bioz Stars, 2020-02
    99/100 stars
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    99
    TaKaRa pegfp n1 vector
    Increased RNA encapsidation in virions of the N17K mutant. (A) For quantification of the vector and helper RNAs packaged in virions of the NC N17K mutant, 293T cells were transfected with <t>pHR′-CMV-GFP,</t> pMD.G, and pHIVgptSVPA expressing the N17K mutant or wild-type Gag. At 2 days posttransfection, RNAs were extracted from virions purified through a 25% sucrose cushion and analyzed by slot blot hybridization with either a GFP (top panel) or a GPT (bottom panel) riboprobe to detect vector and helper RNAs, respectively. To compare the encapsidation of foreign RNAs by wild-type and N17K virions, we transfected 293T cells with <t>pQCXIP-gfp-C1</t> and pHIVgptSVPA expressing the N17K mutant or wild-type Gag. At 2 days posttransfection, total RNAs were extracted from the cells and from virions normalized by exogenous RT. The RNAs were reverse transcribed and the indicated dilutions of cDNA were amplified by PCR with primers specific to GFP. This semiquantitative analysis was performed with virion (B) and cellular (C) samples. Undiluted samples of virion and cellular RNA prior to reverse transcription (no RT) were used to verify the absence of contaminating plasmid DNA (D). PCR samples were electrophoresed in a 2% agarose gel.
    Pegfp N1 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 5871 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pegfp n1 vector - by Bioz Stars, 2020-02
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    99
    TaKaRa pmcherry c1
    Nano-scale dynamics of adhesion proteins in migrating cells (a, b) Live-cell time-lapse multicolor scanning angle interference imaging of epithelial cells expressing paxillin-mEmerald and <t>mCherry-vinculin</t> following cell-cell contact ( a ) or in an assembling adhesion during cell retraction ( b ). The left panels show paxillin epifluorescence images at the indicated time point, the region of interest (ROI) is boxed in red. The middle and right panels show heights of paxillin and vinculin in the ROI. The plots show distributions of paxillin and vinculin heights in ROI, mean height ( H ) above the oxide surface is calculated over 35 to 120nm. In ( a ) the histogram shows the measured heights of paxillin and vinculin at each pixel corresponding to site of adhesion. In ( b ) the histogram displays the heights of the molecules at the subset of ROI pixels within the boundary of the left adhesion (indicated by white arrow). ( c ) The plot shows the relative heights of paxillin and vinculin in maturing adhesions during cell retraction. Axial position is reported relative to the average height of molecules in all adhesions in the cell ( P
    Pmcherry C1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1502 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pmcherry c1 - by Bioz Stars, 2020-02
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    99
    TaKaRa extaq
    Nano-scale dynamics of adhesion proteins in migrating cells (a, b) Live-cell time-lapse multicolor scanning angle interference imaging of epithelial cells expressing paxillin-mEmerald and <t>mCherry-vinculin</t> following cell-cell contact ( a ) or in an assembling adhesion during cell retraction ( b ). The left panels show paxillin epifluorescence images at the indicated time point, the region of interest (ROI) is boxed in red. The middle and right panels show heights of paxillin and vinculin in the ROI. The plots show distributions of paxillin and vinculin heights in ROI, mean height ( H ) above the oxide surface is calculated over 35 to 120nm. In ( a ) the histogram shows the measured heights of paxillin and vinculin at each pixel corresponding to site of adhesion. In ( b ) the histogram displays the heights of the molecules at the subset of ROI pixels within the boundary of the left adhesion (indicated by white arrow). ( c ) The plot shows the relative heights of paxillin and vinculin in maturing adhesions during cell retraction. Axial position is reported relative to the average height of molecules in all adhesions in the cell ( P
    Extaq, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 2207 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 2207 article reviews
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    extaq - by Bioz Stars, 2020-02
    99/100 stars
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    86
    TaKaRa pdsred2 c1
    Nano-scale dynamics of adhesion proteins in migrating cells (a, b) Live-cell time-lapse multicolor scanning angle interference imaging of epithelial cells expressing paxillin-mEmerald and <t>mCherry-vinculin</t> following cell-cell contact ( a ) or in an assembling adhesion during cell retraction ( b ). The left panels show paxillin epifluorescence images at the indicated time point, the region of interest (ROI) is boxed in red. The middle and right panels show heights of paxillin and vinculin in the ROI. The plots show distributions of paxillin and vinculin heights in ROI, mean height ( H ) above the oxide surface is calculated over 35 to 120nm. In ( a ) the histogram shows the measured heights of paxillin and vinculin at each pixel corresponding to site of adhesion. In ( b ) the histogram displays the heights of the molecules at the subset of ROI pixels within the boundary of the left adhesion (indicated by white arrow). ( c ) The plot shows the relative heights of paxillin and vinculin in maturing adhesions during cell retraction. Axial position is reported relative to the average height of molecules in all adhesions in the cell ( P
    Pdsred2 C1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pdsred2 c1 - by Bioz Stars, 2020-02
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    90
    TaKaRa sybr green master mix kits
    Nano-scale dynamics of adhesion proteins in migrating cells (a, b) Live-cell time-lapse multicolor scanning angle interference imaging of epithelial cells expressing paxillin-mEmerald and <t>mCherry-vinculin</t> following cell-cell contact ( a ) or in an assembling adhesion during cell retraction ( b ). The left panels show paxillin epifluorescence images at the indicated time point, the region of interest (ROI) is boxed in red. The middle and right panels show heights of paxillin and vinculin in the ROI. The plots show distributions of paxillin and vinculin heights in ROI, mean height ( H ) above the oxide surface is calculated over 35 to 120nm. In ( a ) the histogram shows the measured heights of paxillin and vinculin at each pixel corresponding to site of adhesion. In ( b ) the histogram displays the heights of the molecules at the subset of ROI pixels within the boundary of the left adhesion (indicated by white arrow). ( c ) The plot shows the relative heights of paxillin and vinculin in maturing adhesions during cell retraction. Axial position is reported relative to the average height of molecules in all adhesions in the cell ( P
    Sybr Green Master Mix Kits, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    sybr green master mix kits - by Bioz Stars, 2020-02
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    90
    TaKaRa bspei
    Nano-scale dynamics of adhesion proteins in migrating cells (a, b) Live-cell time-lapse multicolor scanning angle interference imaging of epithelial cells expressing paxillin-mEmerald and <t>mCherry-vinculin</t> following cell-cell contact ( a ) or in an assembling adhesion during cell retraction ( b ). The left panels show paxillin epifluorescence images at the indicated time point, the region of interest (ROI) is boxed in red. The middle and right panels show heights of paxillin and vinculin in the ROI. The plots show distributions of paxillin and vinculin heights in ROI, mean height ( H ) above the oxide surface is calculated over 35 to 120nm. In ( a ) the histogram shows the measured heights of paxillin and vinculin at each pixel corresponding to site of adhesion. In ( b ) the histogram displays the heights of the molecules at the subset of ROI pixels within the boundary of the left adhesion (indicated by white arrow). ( c ) The plot shows the relative heights of paxillin and vinculin in maturing adhesions during cell retraction. Axial position is reported relative to the average height of molecules in all adhesions in the cell ( P
    Bspei, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 137 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa pecfp n1 vector
    Nano-scale dynamics of adhesion proteins in migrating cells (a, b) Live-cell time-lapse multicolor scanning angle interference imaging of epithelial cells expressing paxillin-mEmerald and <t>mCherry-vinculin</t> following cell-cell contact ( a ) or in an assembling adhesion during cell retraction ( b ). The left panels show paxillin epifluorescence images at the indicated time point, the region of interest (ROI) is boxed in red. The middle and right panels show heights of paxillin and vinculin in the ROI. The plots show distributions of paxillin and vinculin heights in ROI, mean height ( H ) above the oxide surface is calculated over 35 to 120nm. In ( a ) the histogram shows the measured heights of paxillin and vinculin at each pixel corresponding to site of adhesion. In ( b ) the histogram displays the heights of the molecules at the subset of ROI pixels within the boundary of the left adhesion (indicated by white arrow). ( c ) The plot shows the relative heights of paxillin and vinculin in maturing adhesions during cell retraction. Axial position is reported relative to the average height of molecules in all adhesions in the cell ( P
    Pecfp N1 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 263 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    spei  (TaKaRa)
    90
    TaKaRa spei
    Nano-scale dynamics of adhesion proteins in migrating cells (a, b) Live-cell time-lapse multicolor scanning angle interference imaging of epithelial cells expressing paxillin-mEmerald and <t>mCherry-vinculin</t> following cell-cell contact ( a ) or in an assembling adhesion during cell retraction ( b ). The left panels show paxillin epifluorescence images at the indicated time point, the region of interest (ROI) is boxed in red. The middle and right panels show heights of paxillin and vinculin in the ROI. The plots show distributions of paxillin and vinculin heights in ROI, mean height ( H ) above the oxide surface is calculated over 35 to 120nm. In ( a ) the histogram shows the measured heights of paxillin and vinculin at each pixel corresponding to site of adhesion. In ( b ) the histogram displays the heights of the molecules at the subset of ROI pixels within the boundary of the left adhesion (indicated by white arrow). ( c ) The plot shows the relative heights of paxillin and vinculin in maturing adhesions during cell retraction. Axial position is reported relative to the average height of molecules in all adhesions in the cell ( P
    Spei, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 395 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    TaKaRa bsp1407i
    Nano-scale dynamics of adhesion proteins in migrating cells (a, b) Live-cell time-lapse multicolor scanning angle interference imaging of epithelial cells expressing paxillin-mEmerald and <t>mCherry-vinculin</t> following cell-cell contact ( a ) or in an assembling adhesion during cell retraction ( b ). The left panels show paxillin epifluorescence images at the indicated time point, the region of interest (ROI) is boxed in red. The middle and right panels show heights of paxillin and vinculin in the ROI. The plots show distributions of paxillin and vinculin heights in ROI, mean height ( H ) above the oxide surface is calculated over 35 to 120nm. In ( a ) the histogram shows the measured heights of paxillin and vinculin at each pixel corresponding to site of adhesion. In ( b ) the histogram displays the heights of the molecules at the subset of ROI pixels within the boundary of the left adhesion (indicated by white arrow). ( c ) The plot shows the relative heights of paxillin and vinculin in maturing adhesions during cell retraction. Axial position is reported relative to the average height of molecules in all adhesions in the cell ( P
    Bsp1407i, supplied by TaKaRa, used in various techniques. Bioz Stars score: 80/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    TaKaRa vector peyfp c1
    Nano-scale dynamics of adhesion proteins in migrating cells (a, b) Live-cell time-lapse multicolor scanning angle interference imaging of epithelial cells expressing paxillin-mEmerald and <t>mCherry-vinculin</t> following cell-cell contact ( a ) or in an assembling adhesion during cell retraction ( b ). The left panels show paxillin epifluorescence images at the indicated time point, the region of interest (ROI) is boxed in red. The middle and right panels show heights of paxillin and vinculin in the ROI. The plots show distributions of paxillin and vinculin heights in ROI, mean height ( H ) above the oxide surface is calculated over 35 to 120nm. In ( a ) the histogram shows the measured heights of paxillin and vinculin at each pixel corresponding to site of adhesion. In ( b ) the histogram displays the heights of the molecules at the subset of ROI pixels within the boundary of the left adhesion (indicated by white arrow). ( c ) The plot shows the relative heights of paxillin and vinculin in maturing adhesions during cell retraction. Axial position is reported relative to the average height of molecules in all adhesions in the cell ( P
    Vector Peyfp C1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 87/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nano-scale dynamics of adhesion proteins in migrating cells (a, b) Live-cell time-lapse multicolor scanning angle interference imaging of epithelial cells expressing paxillin-mEmerald and <t>mCherry-vinculin</t> following cell-cell contact ( a ) or in an assembling adhesion during cell retraction ( b ). The left panels show paxillin epifluorescence images at the indicated time point, the region of interest (ROI) is boxed in red. The middle and right panels show heights of paxillin and vinculin in the ROI. The plots show distributions of paxillin and vinculin heights in ROI, mean height ( H ) above the oxide surface is calculated over 35 to 120nm. In ( a ) the histogram shows the measured heights of paxillin and vinculin at each pixel corresponding to site of adhesion. In ( b ) the histogram displays the heights of the molecules at the subset of ROI pixels within the boundary of the left adhesion (indicated by white arrow). ( c ) The plot shows the relative heights of paxillin and vinculin in maturing adhesions during cell retraction. Axial position is reported relative to the average height of molecules in all adhesions in the cell ( P
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    Nano-scale dynamics of adhesion proteins in migrating cells (a, b) Live-cell time-lapse multicolor scanning angle interference imaging of epithelial cells expressing paxillin-mEmerald and <t>mCherry-vinculin</t> following cell-cell contact ( a ) or in an assembling adhesion during cell retraction ( b ). The left panels show paxillin epifluorescence images at the indicated time point, the region of interest (ROI) is boxed in red. The middle and right panels show heights of paxillin and vinculin in the ROI. The plots show distributions of paxillin and vinculin heights in ROI, mean height ( H ) above the oxide surface is calculated over 35 to 120nm. In ( a ) the histogram shows the measured heights of paxillin and vinculin at each pixel corresponding to site of adhesion. In ( b ) the histogram displays the heights of the molecules at the subset of ROI pixels within the boundary of the left adhesion (indicated by white arrow). ( c ) The plot shows the relative heights of paxillin and vinculin in maturing adhesions during cell retraction. Axial position is reported relative to the average height of molecules in all adhesions in the cell ( P
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    Nano-scale dynamics of adhesion proteins in migrating cells (a, b) Live-cell time-lapse multicolor scanning angle interference imaging of epithelial cells expressing paxillin-mEmerald and <t>mCherry-vinculin</t> following cell-cell contact ( a ) or in an assembling adhesion during cell retraction ( b ). The left panels show paxillin epifluorescence images at the indicated time point, the region of interest (ROI) is boxed in red. The middle and right panels show heights of paxillin and vinculin in the ROI. The plots show distributions of paxillin and vinculin heights in ROI, mean height ( H ) above the oxide surface is calculated over 35 to 120nm. In ( a ) the histogram shows the measured heights of paxillin and vinculin at each pixel corresponding to site of adhesion. In ( b ) the histogram displays the heights of the molecules at the subset of ROI pixels within the boundary of the left adhesion (indicated by white arrow). ( c ) The plot shows the relative heights of paxillin and vinculin in maturing adhesions during cell retraction. Axial position is reported relative to the average height of molecules in all adhesions in the cell ( P
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    Image Search Results


    Single-Cell Electroporation Can Be Carried Out at High Efficiency by Front-Loading Micropipettes. (a) In a micropipette filled with saline (scale bar 1 mm), a front-loaded reagent diffuses over time, decreasing concentration of the sample at the tip (drawings are not to scale). (b) Approximately 2 nL of three different fluorescent molecules, Alexa Fluor 594 (Dye1) and 488 (Dye2) hydrazide salts and SYBR-Green-labeled pEGFP-N1 (Plasmid), were front-loaded into micropipettes and their fluorescence monitored at 1 minute intervals over ten minutes and compared to simulations (continuous lines). Each data point is the mean ± s.d. of three independent experiments. (c) Concentration of plasmid in the tip of the micropipette remains stable enough to reliably transfect multiple cells with pEGFP-N1. 79.1±8.7% of the transfected cells expressed EGFP 24 hours following transfection ( n = 72 from six independent experiments, where 12 cells were transfected within 2 minutes in each experiment). Scale bar 50 µm.

    Journal: PLoS ONE

    Article Title: High-Throughput Single-Cell Manipulation in Brain Tissue

    doi: 10.1371/journal.pone.0035603

    Figure Lengend Snippet: Single-Cell Electroporation Can Be Carried Out at High Efficiency by Front-Loading Micropipettes. (a) In a micropipette filled with saline (scale bar 1 mm), a front-loaded reagent diffuses over time, decreasing concentration of the sample at the tip (drawings are not to scale). (b) Approximately 2 nL of three different fluorescent molecules, Alexa Fluor 594 (Dye1) and 488 (Dye2) hydrazide salts and SYBR-Green-labeled pEGFP-N1 (Plasmid), were front-loaded into micropipettes and their fluorescence monitored at 1 minute intervals over ten minutes and compared to simulations (continuous lines). Each data point is the mean ± s.d. of three independent experiments. (c) Concentration of plasmid in the tip of the micropipette remains stable enough to reliably transfect multiple cells with pEGFP-N1. 79.1±8.7% of the transfected cells expressed EGFP 24 hours following transfection ( n = 72 from six independent experiments, where 12 cells were transfected within 2 minutes in each experiment). Scale bar 50 µm.

    Article Snippet: All plasmids were acquired from Addgene unless otherwise specified: pCAG-EGFP, pCAG-YFP, pCAG-dsRed (Addgene plasmids 11150, 11180, and 11151, respectively) pEGFP-N1 (Clontech) pCI-tdTomato, (courtesy of Rachael Neve) mCherry Lac-REP (Addgene plasmid 18985) Cerulean (Addgene plasmid 15214) pEAK10-His-Myc-Kal7 (Addgene plasmid 25454) pEAK10-His-Myc-Kal5 and pEAK10-His-Myc-Kal9 (Addgene plasmids 25440 and 25441, respectively) pCAG-Cerulean was constructed by removing the Cerulean gene from its native Clontech backbone using the AgeI and BsrGI restriction endonucleases (New England Biolabs) and sub-cloning into the pCAG plasmid.

    Techniques: Electroporation, Concentration Assay, SYBR Green Assay, Labeling, Plasmid Preparation, Fluorescence, Transfection

    Increased RNA encapsidation in virions of the N17K mutant. (A) For quantification of the vector and helper RNAs packaged in virions of the NC N17K mutant, 293T cells were transfected with pHR′-CMV-GFP, pMD.G, and pHIVgptSVPA expressing the N17K mutant or wild-type Gag. At 2 days posttransfection, RNAs were extracted from virions purified through a 25% sucrose cushion and analyzed by slot blot hybridization with either a GFP (top panel) or a GPT (bottom panel) riboprobe to detect vector and helper RNAs, respectively. To compare the encapsidation of foreign RNAs by wild-type and N17K virions, we transfected 293T cells with pQCXIP-gfp-C1 and pHIVgptSVPA expressing the N17K mutant or wild-type Gag. At 2 days posttransfection, total RNAs were extracted from the cells and from virions normalized by exogenous RT. The RNAs were reverse transcribed and the indicated dilutions of cDNA were amplified by PCR with primers specific to GFP. This semiquantitative analysis was performed with virion (B) and cellular (C) samples. Undiluted samples of virion and cellular RNA prior to reverse transcription (no RT) were used to verify the absence of contaminating plasmid DNA (D). PCR samples were electrophoresed in a 2% agarose gel.

    Journal: Journal of Virology

    Article Title: Single Point Mutations in the Zinc Finger Motifs of the Human Immunodeficiency Virus Type 1 Nucleocapsid Alter RNA Binding Specificities of the Gag Protein and Enhance Packaging and Infectivity

    doi: 10.1128/JVI.79.12.7756-7767.2005

    Figure Lengend Snippet: Increased RNA encapsidation in virions of the N17K mutant. (A) For quantification of the vector and helper RNAs packaged in virions of the NC N17K mutant, 293T cells were transfected with pHR′-CMV-GFP, pMD.G, and pHIVgptSVPA expressing the N17K mutant or wild-type Gag. At 2 days posttransfection, RNAs were extracted from virions purified through a 25% sucrose cushion and analyzed by slot blot hybridization with either a GFP (top panel) or a GPT (bottom panel) riboprobe to detect vector and helper RNAs, respectively. To compare the encapsidation of foreign RNAs by wild-type and N17K virions, we transfected 293T cells with pQCXIP-gfp-C1 and pHIVgptSVPA expressing the N17K mutant or wild-type Gag. At 2 days posttransfection, total RNAs were extracted from the cells and from virions normalized by exogenous RT. The RNAs were reverse transcribed and the indicated dilutions of cDNA were amplified by PCR with primers specific to GFP. This semiquantitative analysis was performed with virion (B) and cellular (C) samples. Undiluted samples of virion and cellular RNA prior to reverse transcription (no RT) were used to verify the absence of contaminating plasmid DNA (D). PCR samples were electrophoresed in a 2% agarose gel.

    Article Snippet: An MoMLV-based vector expressing GFP (pQCXIP-gfp-C1) was created by cloning a cDNA encoding GFP into pQCXIP (Clontech) at AgeI and EcoRI sites.

    Techniques: Mutagenesis, Plasmid Preparation, Transfection, Expressing, Purification, Dot Blot, Hybridization, Amplification, Polymerase Chain Reaction, Agarose Gel Electrophoresis

    Nano-scale dynamics of adhesion proteins in migrating cells (a, b) Live-cell time-lapse multicolor scanning angle interference imaging of epithelial cells expressing paxillin-mEmerald and mCherry-vinculin following cell-cell contact ( a ) or in an assembling adhesion during cell retraction ( b ). The left panels show paxillin epifluorescence images at the indicated time point, the region of interest (ROI) is boxed in red. The middle and right panels show heights of paxillin and vinculin in the ROI. The plots show distributions of paxillin and vinculin heights in ROI, mean height ( H ) above the oxide surface is calculated over 35 to 120nm. In ( a ) the histogram shows the measured heights of paxillin and vinculin at each pixel corresponding to site of adhesion. In ( b ) the histogram displays the heights of the molecules at the subset of ROI pixels within the boundary of the left adhesion (indicated by white arrow). ( c ) The plot shows the relative heights of paxillin and vinculin in maturing adhesions during cell retraction. Axial position is reported relative to the average height of molecules in all adhesions in the cell ( P

    Journal: Nature methods

    Article Title: Scanning Angle Interference Microscopy Reveals Cell Dynamics at the Nano-scale

    doi: 10.1038/nmeth.2077

    Figure Lengend Snippet: Nano-scale dynamics of adhesion proteins in migrating cells (a, b) Live-cell time-lapse multicolor scanning angle interference imaging of epithelial cells expressing paxillin-mEmerald and mCherry-vinculin following cell-cell contact ( a ) or in an assembling adhesion during cell retraction ( b ). The left panels show paxillin epifluorescence images at the indicated time point, the region of interest (ROI) is boxed in red. The middle and right panels show heights of paxillin and vinculin in the ROI. The plots show distributions of paxillin and vinculin heights in ROI, mean height ( H ) above the oxide surface is calculated over 35 to 120nm. In ( a ) the histogram shows the measured heights of paxillin and vinculin at each pixel corresponding to site of adhesion. In ( b ) the histogram displays the heights of the molecules at the subset of ROI pixels within the boundary of the left adhesion (indicated by white arrow). ( c ) The plot shows the relative heights of paxillin and vinculin in maturing adhesions during cell retraction. Axial position is reported relative to the average height of molecules in all adhesions in the cell ( P

    Article Snippet: Murine talin1 tagged with mCherry at the N-terminus and mEmerald at the C-terminus was prepared from mCherry N-terminally and mEmerald C-terminally tagged murine Talin1 constructs previously described ligating a SalI-NotI fragment from the mEmerald construct containing the C-terminal end of talin1 fused to mEmerald with a AgeI-SalI fragment from the mCherry construct containing mCherry fused to the N-terminal end of talin1 with the NotI-AgeI vector fragment from pmCherry-N1 (Clontech).

    Techniques: Imaging, Expressing