agei Evrogen Search Results


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  • 95
    Qiagen maxi kit
    Maxi Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 377 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/maxi kit/product/Qiagen
    Average 95 stars, based on 377 article reviews
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    maxi kit - by Bioz Stars, 2020-02
    95/100 stars
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    90
    Thermo Fisher ecori
    Ecori, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 9775 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 9775 article reviews
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    ecori - by Bioz Stars, 2020-02
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    79
    Evrogen pturbogfp mito
    Pturbogfp Mito, supplied by Evrogen, used in various techniques. Bioz Stars score: 79/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Evrogen pmkate2 h2b
    Pmkate2 H2b, supplied by Evrogen, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 12 article reviews
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    99
    Evrogen pturbogfp n vector
    Pturbogfp N Vector, supplied by Evrogen, used in various techniques. Bioz Stars score: 99/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Evrogen dendra2 protein
    Dendra2 Protein, supplied by Evrogen, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Evrogen pkillerred dmito vector
    Pkillerred Dmito Vector, supplied by Evrogen, used in various techniques. Bioz Stars score: 89/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Evrogen ptag h2b bfp
    Ptag H2b Bfp, supplied by Evrogen, used in various techniques. Bioz Stars score: 84/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 84 stars, based on 34 article reviews
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    99
    Evrogen bglii
    Bglii, supplied by Evrogen, used in various techniques. Bioz Stars score: 99/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bglii - by Bioz Stars, 2020-02
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    96
    Evrogen tagbfp n1
    Tagbfp N1, supplied by Evrogen, used in various techniques. Bioz Stars score: 96/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Evrogen turborfp
    Turborfp, supplied by Evrogen, used in various techniques. Bioz Stars score: 99/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Evrogen turborfp n
    Turborfp N, supplied by Evrogen, used in various techniques. Bioz Stars score: 78/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Evrogen ptgfp prl
    Ptgfp Prl, supplied by Evrogen, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Evrogen turbogfp
    Turbogfp, supplied by Evrogen, used in various techniques. Bioz Stars score: 99/100, based on 172 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Evrogen tagbfp
    Src released from UNC119 by Arl2/3 is trapped at the RE to be transported to the PM. a Representative time-lapse sequences of fluorescence distribution of HeLa cells co-expressing Src-mCh ( first and third rows ) and <t>SrcWT-paGFP</t> ( second and fourth rows ) in the absence ( first and second rows ) or presence ( third and fourth rows ) of <t>UNC119A-TagBFP</t> ectopic expression. Src-paGFP was photoactivated at 0 s in the perinuclear region and subsequently Src-mCh was photobleached at 400 s in the same area (photoactivation/photobleach area: red outline ). b Segmentation of cells in ( a ): RE ( red ), PM ( blue outline ) and communicating cytoplasm ( green ). The ratio of Src-paGFP fluorescence per segment (RE, red ; PM, blue ; cytoplasm, green ) over total fluorescence in the cell and normalised to the respective ratio of Src-mCh fluorescence was plotted against time. The PM ratio was fitted to a mono-exponential recovery ( black line ). c Average traces of FLAP and FRAP curves fitted to the compartmental model described in Methods. Loss of Src-paGFP fluorescence at the perinuclear region normalised to whole-cell Src-paGFP fluorescence ( blue and cyan curves ) and corresponding gain of Src-mCh fluorescence after photobleaching at the same ROI, normalised to whole-cell Src-mCh fluorescence ( red and orange curves ). Control cells: mean ± SEM; n = 5 cells ( blue and red curves ), UNC119 ectopic expression: mean ± SEM; n = 6 cells ( cyan and orange curves ). Scale bars , 10 μm
    Tagbfp, supplied by Evrogen, used in various techniques. Bioz Stars score: 99/100, based on 212 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    76
    Evrogen pdendra2 plasmid
    Src released from UNC119 by Arl2/3 is trapped at the RE to be transported to the PM. a Representative time-lapse sequences of fluorescence distribution of HeLa cells co-expressing Src-mCh ( first and third rows ) and <t>SrcWT-paGFP</t> ( second and fourth rows ) in the absence ( first and second rows ) or presence ( third and fourth rows ) of <t>UNC119A-TagBFP</t> ectopic expression. Src-paGFP was photoactivated at 0 s in the perinuclear region and subsequently Src-mCh was photobleached at 400 s in the same area (photoactivation/photobleach area: red outline ). b Segmentation of cells in ( a ): RE ( red ), PM ( blue outline ) and communicating cytoplasm ( green ). The ratio of Src-paGFP fluorescence per segment (RE, red ; PM, blue ; cytoplasm, green ) over total fluorescence in the cell and normalised to the respective ratio of Src-mCh fluorescence was plotted against time. The PM ratio was fitted to a mono-exponential recovery ( black line ). c Average traces of FLAP and FRAP curves fitted to the compartmental model described in Methods. Loss of Src-paGFP fluorescence at the perinuclear region normalised to whole-cell Src-paGFP fluorescence ( blue and cyan curves ) and corresponding gain of Src-mCh fluorescence after photobleaching at the same ROI, normalised to whole-cell Src-mCh fluorescence ( red and orange curves ). Control cells: mean ± SEM; n = 5 cells ( blue and red curves ), UNC119 ectopic expression: mean ± SEM; n = 6 cells ( cyan and orange curves ). Scale bars , 10 μm
    Pdendra2 Plasmid, supplied by Evrogen, used in various techniques. Bioz Stars score: 76/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nhei  (TaKaRa)
    90
    TaKaRa nhei
    Src released from UNC119 by Arl2/3 is trapped at the RE to be transported to the PM. a Representative time-lapse sequences of fluorescence distribution of HeLa cells co-expressing Src-mCh ( first and third rows ) and <t>SrcWT-paGFP</t> ( second and fourth rows ) in the absence ( first and second rows ) or presence ( third and fourth rows ) of <t>UNC119A-TagBFP</t> ectopic expression. Src-paGFP was photoactivated at 0 s in the perinuclear region and subsequently Src-mCh was photobleached at 400 s in the same area (photoactivation/photobleach area: red outline ). b Segmentation of cells in ( a ): RE ( red ), PM ( blue outline ) and communicating cytoplasm ( green ). The ratio of Src-paGFP fluorescence per segment (RE, red ; PM, blue ; cytoplasm, green ) over total fluorescence in the cell and normalised to the respective ratio of Src-mCh fluorescence was plotted against time. The PM ratio was fitted to a mono-exponential recovery ( black line ). c Average traces of FLAP and FRAP curves fitted to the compartmental model described in Methods. Loss of Src-paGFP fluorescence at the perinuclear region normalised to whole-cell Src-paGFP fluorescence ( blue and cyan curves ) and corresponding gain of Src-mCh fluorescence after photobleaching at the same ROI, normalised to whole-cell Src-mCh fluorescence ( red and orange curves ). Control cells: mean ± SEM; n = 5 cells ( blue and red curves ), UNC119 ectopic expression: mean ± SEM; n = 6 cells ( cyan and orange curves ). Scale bars , 10 μm
    Nhei, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 1437 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Evrogen ptagrfp mito
    Src released from UNC119 by Arl2/3 is trapped at the RE to be transported to the PM. a Representative time-lapse sequences of fluorescence distribution of HeLa cells co-expressing Src-mCh ( first and third rows ) and <t>SrcWT-paGFP</t> ( second and fourth rows ) in the absence ( first and second rows ) or presence ( third and fourth rows ) of <t>UNC119A-TagBFP</t> ectopic expression. Src-paGFP was photoactivated at 0 s in the perinuclear region and subsequently Src-mCh was photobleached at 400 s in the same area (photoactivation/photobleach area: red outline ). b Segmentation of cells in ( a ): RE ( red ), PM ( blue outline ) and communicating cytoplasm ( green ). The ratio of Src-paGFP fluorescence per segment (RE, red ; PM, blue ; cytoplasm, green ) over total fluorescence in the cell and normalised to the respective ratio of Src-mCh fluorescence was plotted against time. The PM ratio was fitted to a mono-exponential recovery ( black line ). c Average traces of FLAP and FRAP curves fitted to the compartmental model described in Methods. Loss of Src-paGFP fluorescence at the perinuclear region normalised to whole-cell Src-paGFP fluorescence ( blue and cyan curves ) and corresponding gain of Src-mCh fluorescence after photobleaching at the same ROI, normalised to whole-cell Src-mCh fluorescence ( red and orange curves ). Control cells: mean ± SEM; n = 5 cells ( blue and red curves ), UNC119 ectopic expression: mean ± SEM; n = 6 cells ( cyan and orange curves ). Scale bars , 10 μm
    Ptagrfp Mito, supplied by Evrogen, used in various techniques. Bioz Stars score: 99/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Evrogen cdnas
    Src released from UNC119 by Arl2/3 is trapped at the RE to be transported to the PM. a Representative time-lapse sequences of fluorescence distribution of HeLa cells co-expressing Src-mCh ( first and third rows ) and <t>SrcWT-paGFP</t> ( second and fourth rows ) in the absence ( first and second rows ) or presence ( third and fourth rows ) of <t>UNC119A-TagBFP</t> ectopic expression. Src-paGFP was photoactivated at 0 s in the perinuclear region and subsequently Src-mCh was photobleached at 400 s in the same area (photoactivation/photobleach area: red outline ). b Segmentation of cells in ( a ): RE ( red ), PM ( blue outline ) and communicating cytoplasm ( green ). The ratio of Src-paGFP fluorescence per segment (RE, red ; PM, blue ; cytoplasm, green ) over total fluorescence in the cell and normalised to the respective ratio of Src-mCh fluorescence was plotted against time. The PM ratio was fitted to a mono-exponential recovery ( black line ). c Average traces of FLAP and FRAP curves fitted to the compartmental model described in Methods. Loss of Src-paGFP fluorescence at the perinuclear region normalised to whole-cell Src-paGFP fluorescence ( blue and cyan curves ) and corresponding gain of Src-mCh fluorescence after photobleaching at the same ROI, normalised to whole-cell Src-mCh fluorescence ( red and orange curves ). Control cells: mean ± SEM; n = 5 cells ( blue and red curves ), UNC119 ectopic expression: mean ± SEM; n = 6 cells ( cyan and orange curves ). Scale bars , 10 μm
    Cdnas, supplied by Evrogen, used in various techniques. Bioz Stars score: 99/100, based on 115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    TaKaRa pacgfp1
    Src released from UNC119 by Arl2/3 is trapped at the RE to be transported to the PM. a Representative time-lapse sequences of fluorescence distribution of HeLa cells co-expressing Src-mCh ( first and third rows ) and <t>SrcWT-paGFP</t> ( second and fourth rows ) in the absence ( first and second rows ) or presence ( third and fourth rows ) of <t>UNC119A-TagBFP</t> ectopic expression. Src-paGFP was photoactivated at 0 s in the perinuclear region and subsequently Src-mCh was photobleached at 400 s in the same area (photoactivation/photobleach area: red outline ). b Segmentation of cells in ( a ): RE ( red ), PM ( blue outline ) and communicating cytoplasm ( green ). The ratio of Src-paGFP fluorescence per segment (RE, red ; PM, blue ; cytoplasm, green ) over total fluorescence in the cell and normalised to the respective ratio of Src-mCh fluorescence was plotted against time. The PM ratio was fitted to a mono-exponential recovery ( black line ). c Average traces of FLAP and FRAP curves fitted to the compartmental model described in Methods. Loss of Src-paGFP fluorescence at the perinuclear region normalised to whole-cell Src-paGFP fluorescence ( blue and cyan curves ) and corresponding gain of Src-mCh fluorescence after photobleaching at the same ROI, normalised to whole-cell Src-mCh fluorescence ( red and orange curves ). Control cells: mean ± SEM; n = 5 cells ( blue and red curves ), UNC119 ectopic expression: mean ± SEM; n = 6 cells ( cyan and orange curves ). Scale bars , 10 μm
    Pacgfp1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 83/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa bspei
    Src released from UNC119 by Arl2/3 is trapped at the RE to be transported to the PM. a Representative time-lapse sequences of fluorescence distribution of HeLa cells co-expressing Src-mCh ( first and third rows ) and <t>SrcWT-paGFP</t> ( second and fourth rows ) in the absence ( first and second rows ) or presence ( third and fourth rows ) of <t>UNC119A-TagBFP</t> ectopic expression. Src-paGFP was photoactivated at 0 s in the perinuclear region and subsequently Src-mCh was photobleached at 400 s in the same area (photoactivation/photobleach area: red outline ). b Segmentation of cells in ( a ): RE ( red ), PM ( blue outline ) and communicating cytoplasm ( green ). The ratio of Src-paGFP fluorescence per segment (RE, red ; PM, blue ; cytoplasm, green ) over total fluorescence in the cell and normalised to the respective ratio of Src-mCh fluorescence was plotted against time. The PM ratio was fitted to a mono-exponential recovery ( black line ). c Average traces of FLAP and FRAP curves fitted to the compartmental model described in Methods. Loss of Src-paGFP fluorescence at the perinuclear region normalised to whole-cell Src-paGFP fluorescence ( blue and cyan curves ) and corresponding gain of Src-mCh fluorescence after photobleaching at the same ROI, normalised to whole-cell Src-mCh fluorescence ( red and orange curves ). Control cells: mean ± SEM; n = 5 cells ( blue and red curves ), UNC119 ectopic expression: mean ± SEM; n = 6 cells ( cyan and orange curves ). Scale bars , 10 μm
    Bspei, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 137 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Evrogen phyper dmito vector
    Src released from UNC119 by Arl2/3 is trapped at the RE to be transported to the PM. a Representative time-lapse sequences of fluorescence distribution of HeLa cells co-expressing Src-mCh ( first and third rows ) and <t>SrcWT-paGFP</t> ( second and fourth rows ) in the absence ( first and second rows ) or presence ( third and fourth rows ) of <t>UNC119A-TagBFP</t> ectopic expression. Src-paGFP was photoactivated at 0 s in the perinuclear region and subsequently Src-mCh was photobleached at 400 s in the same area (photoactivation/photobleach area: red outline ). b Segmentation of cells in ( a ): RE ( red ), PM ( blue outline ) and communicating cytoplasm ( green ). The ratio of Src-paGFP fluorescence per segment (RE, red ; PM, blue ; cytoplasm, green ) over total fluorescence in the cell and normalised to the respective ratio of Src-mCh fluorescence was plotted against time. The PM ratio was fitted to a mono-exponential recovery ( black line ). c Average traces of FLAP and FRAP curves fitted to the compartmental model described in Methods. Loss of Src-paGFP fluorescence at the perinuclear region normalised to whole-cell Src-paGFP fluorescence ( blue and cyan curves ) and corresponding gain of Src-mCh fluorescence after photobleaching at the same ROI, normalised to whole-cell Src-mCh fluorescence ( red and orange curves ). Control cells: mean ± SEM; n = 5 cells ( blue and red curves ), UNC119 ectopic expression: mean ± SEM; n = 6 cells ( cyan and orange curves ). Scale bars , 10 μm
    Phyper Dmito Vector, supplied by Evrogen, used in various techniques. Bioz Stars score: 85/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Evrogen ptag h2b apple
    Src released from UNC119 by Arl2/3 is trapped at the RE to be transported to the PM. a Representative time-lapse sequences of fluorescence distribution of HeLa cells co-expressing Src-mCh ( first and third rows ) and <t>SrcWT-paGFP</t> ( second and fourth rows ) in the absence ( first and second rows ) or presence ( third and fourth rows ) of <t>UNC119A-TagBFP</t> ectopic expression. Src-paGFP was photoactivated at 0 s in the perinuclear region and subsequently Src-mCh was photobleached at 400 s in the same area (photoactivation/photobleach area: red outline ). b Segmentation of cells in ( a ): RE ( red ), PM ( blue outline ) and communicating cytoplasm ( green ). The ratio of Src-paGFP fluorescence per segment (RE, red ; PM, blue ; cytoplasm, green ) over total fluorescence in the cell and normalised to the respective ratio of Src-mCh fluorescence was plotted against time. The PM ratio was fitted to a mono-exponential recovery ( black line ). c Average traces of FLAP and FRAP curves fitted to the compartmental model described in Methods. Loss of Src-paGFP fluorescence at the perinuclear region normalised to whole-cell Src-paGFP fluorescence ( blue and cyan curves ) and corresponding gain of Src-mCh fluorescence after photobleaching at the same ROI, normalised to whole-cell Src-mCh fluorescence ( red and orange curves ). Control cells: mean ± SEM; n = 5 cells ( blue and red curves ), UNC119 ectopic expression: mean ± SEM; n = 6 cells ( cyan and orange curves ). Scale bars , 10 μm
    Ptag H2b Apple, supplied by Evrogen, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa mcherry n1
    Src released from UNC119 by Arl2/3 is trapped at the RE to be transported to the PM. a Representative time-lapse sequences of fluorescence distribution of HeLa cells co-expressing Src-mCh ( first and third rows ) and <t>SrcWT-paGFP</t> ( second and fourth rows ) in the absence ( first and second rows ) or presence ( third and fourth rows ) of <t>UNC119A-TagBFP</t> ectopic expression. Src-paGFP was photoactivated at 0 s in the perinuclear region and subsequently Src-mCh was photobleached at 400 s in the same area (photoactivation/photobleach area: red outline ). b Segmentation of cells in ( a ): RE ( red ), PM ( blue outline ) and communicating cytoplasm ( green ). The ratio of Src-paGFP fluorescence per segment (RE, red ; PM, blue ; cytoplasm, green ) over total fluorescence in the cell and normalised to the respective ratio of Src-mCh fluorescence was plotted against time. The PM ratio was fitted to a mono-exponential recovery ( black line ). c Average traces of FLAP and FRAP curves fitted to the compartmental model described in Methods. Loss of Src-paGFP fluorescence at the perinuclear region normalised to whole-cell Src-paGFP fluorescence ( blue and cyan curves ) and corresponding gain of Src-mCh fluorescence after photobleaching at the same ROI, normalised to whole-cell Src-mCh fluorescence ( red and orange curves ). Control cells: mean ± SEM; n = 5 cells ( blue and red curves ), UNC119 ectopic expression: mean ± SEM; n = 6 cells ( cyan and orange curves ). Scale bars , 10 μm
    Mcherry N1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 120 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Evrogen pcr amplified
    Src released from UNC119 by Arl2/3 is trapped at the RE to be transported to the PM. a Representative time-lapse sequences of fluorescence distribution of HeLa cells co-expressing Src-mCh ( first and third rows ) and <t>SrcWT-paGFP</t> ( second and fourth rows ) in the absence ( first and second rows ) or presence ( third and fourth rows ) of <t>UNC119A-TagBFP</t> ectopic expression. Src-paGFP was photoactivated at 0 s in the perinuclear region and subsequently Src-mCh was photobleached at 400 s in the same area (photoactivation/photobleach area: red outline ). b Segmentation of cells in ( a ): RE ( red ), PM ( blue outline ) and communicating cytoplasm ( green ). The ratio of Src-paGFP fluorescence per segment (RE, red ; PM, blue ; cytoplasm, green ) over total fluorescence in the cell and normalised to the respective ratio of Src-mCh fluorescence was plotted against time. The PM ratio was fitted to a mono-exponential recovery ( black line ). c Average traces of FLAP and FRAP curves fitted to the compartmental model described in Methods. Loss of Src-paGFP fluorescence at the perinuclear region normalised to whole-cell Src-paGFP fluorescence ( blue and cyan curves ) and corresponding gain of Src-mCh fluorescence after photobleaching at the same ROI, normalised to whole-cell Src-mCh fluorescence ( red and orange curves ). Control cells: mean ± SEM; n = 5 cells ( blue and red curves ), UNC119 ectopic expression: mean ± SEM; n = 6 cells ( cyan and orange curves ). Scale bars , 10 μm
    Pcr Amplified, supplied by Evrogen, used in various techniques. Bioz Stars score: 99/100, based on 113 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    OriGene bspei
    Src released from UNC119 by Arl2/3 is trapped at the RE to be transported to the PM. a Representative time-lapse sequences of fluorescence distribution of HeLa cells co-expressing Src-mCh ( first and third rows ) and <t>SrcWT-paGFP</t> ( second and fourth rows ) in the absence ( first and second rows ) or presence ( third and fourth rows ) of <t>UNC119A-TagBFP</t> ectopic expression. Src-paGFP was photoactivated at 0 s in the perinuclear region and subsequently Src-mCh was photobleached at 400 s in the same area (photoactivation/photobleach area: red outline ). b Segmentation of cells in ( a ): RE ( red ), PM ( blue outline ) and communicating cytoplasm ( green ). The ratio of Src-paGFP fluorescence per segment (RE, red ; PM, blue ; cytoplasm, green ) over total fluorescence in the cell and normalised to the respective ratio of Src-mCh fluorescence was plotted against time. The PM ratio was fitted to a mono-exponential recovery ( black line ). c Average traces of FLAP and FRAP curves fitted to the compartmental model described in Methods. Loss of Src-paGFP fluorescence at the perinuclear region normalised to whole-cell Src-paGFP fluorescence ( blue and cyan curves ) and corresponding gain of Src-mCh fluorescence after photobleaching at the same ROI, normalised to whole-cell Src-mCh fluorescence ( red and orange curves ). Control cells: mean ± SEM; n = 5 cells ( blue and red curves ), UNC119 ectopic expression: mean ± SEM; n = 6 cells ( cyan and orange curves ). Scale bars , 10 μm
    Bspei, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Src released from UNC119 by Arl2/3 is trapped at the RE to be transported to the PM. a Representative time-lapse sequences of fluorescence distribution of HeLa cells co-expressing Src-mCh ( first and third rows ) and <t>SrcWT-paGFP</t> ( second and fourth rows ) in the absence ( first and second rows ) or presence ( third and fourth rows ) of <t>UNC119A-TagBFP</t> ectopic expression. Src-paGFP was photoactivated at 0 s in the perinuclear region and subsequently Src-mCh was photobleached at 400 s in the same area (photoactivation/photobleach area: red outline ). b Segmentation of cells in ( a ): RE ( red ), PM ( blue outline ) and communicating cytoplasm ( green ). The ratio of Src-paGFP fluorescence per segment (RE, red ; PM, blue ; cytoplasm, green ) over total fluorescence in the cell and normalised to the respective ratio of Src-mCh fluorescence was plotted against time. The PM ratio was fitted to a mono-exponential recovery ( black line ). c Average traces of FLAP and FRAP curves fitted to the compartmental model described in Methods. Loss of Src-paGFP fluorescence at the perinuclear region normalised to whole-cell Src-paGFP fluorescence ( blue and cyan curves ) and corresponding gain of Src-mCh fluorescence after photobleaching at the same ROI, normalised to whole-cell Src-mCh fluorescence ( red and orange curves ). Control cells: mean ± SEM; n = 5 cells ( blue and red curves ), UNC119 ectopic expression: mean ± SEM; n = 6 cells ( cyan and orange curves ). Scale bars , 10 μm
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    Src released from UNC119 by Arl2/3 is trapped at the RE to be transported to the PM. a Representative time-lapse sequences of fluorescence distribution of HeLa cells co-expressing Src-mCh ( first and third rows ) and <t>SrcWT-paGFP</t> ( second and fourth rows ) in the absence ( first and second rows ) or presence ( third and fourth rows ) of <t>UNC119A-TagBFP</t> ectopic expression. Src-paGFP was photoactivated at 0 s in the perinuclear region and subsequently Src-mCh was photobleached at 400 s in the same area (photoactivation/photobleach area: red outline ). b Segmentation of cells in ( a ): RE ( red ), PM ( blue outline ) and communicating cytoplasm ( green ). The ratio of Src-paGFP fluorescence per segment (RE, red ; PM, blue ; cytoplasm, green ) over total fluorescence in the cell and normalised to the respective ratio of Src-mCh fluorescence was plotted against time. The PM ratio was fitted to a mono-exponential recovery ( black line ). c Average traces of FLAP and FRAP curves fitted to the compartmental model described in Methods. Loss of Src-paGFP fluorescence at the perinuclear region normalised to whole-cell Src-paGFP fluorescence ( blue and cyan curves ) and corresponding gain of Src-mCh fluorescence after photobleaching at the same ROI, normalised to whole-cell Src-mCh fluorescence ( red and orange curves ). Control cells: mean ± SEM; n = 5 cells ( blue and red curves ), UNC119 ectopic expression: mean ± SEM; n = 6 cells ( cyan and orange curves ). Scale bars , 10 μm
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    Src released from UNC119 by Arl2/3 is trapped at the RE to be transported to the PM. a Representative time-lapse sequences of fluorescence distribution of HeLa cells co-expressing Src-mCh ( first and third rows ) and <t>SrcWT-paGFP</t> ( second and fourth rows ) in the absence ( first and second rows ) or presence ( third and fourth rows ) of <t>UNC119A-TagBFP</t> ectopic expression. Src-paGFP was photoactivated at 0 s in the perinuclear region and subsequently Src-mCh was photobleached at 400 s in the same area (photoactivation/photobleach area: red outline ). b Segmentation of cells in ( a ): RE ( red ), PM ( blue outline ) and communicating cytoplasm ( green ). The ratio of Src-paGFP fluorescence per segment (RE, red ; PM, blue ; cytoplasm, green ) over total fluorescence in the cell and normalised to the respective ratio of Src-mCh fluorescence was plotted against time. The PM ratio was fitted to a mono-exponential recovery ( black line ). c Average traces of FLAP and FRAP curves fitted to the compartmental model described in Methods. Loss of Src-paGFP fluorescence at the perinuclear region normalised to whole-cell Src-paGFP fluorescence ( blue and cyan curves ) and corresponding gain of Src-mCh fluorescence after photobleaching at the same ROI, normalised to whole-cell Src-mCh fluorescence ( red and orange curves ). Control cells: mean ± SEM; n = 5 cells ( blue and red curves ), UNC119 ectopic expression: mean ± SEM; n = 6 cells ( cyan and orange curves ). Scale bars , 10 μm
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    Src released from UNC119 by Arl2/3 is trapped at the RE to be transported to the PM. a Representative time-lapse sequences of fluorescence distribution of HeLa cells co-expressing Src-mCh ( first and third rows ) and <t>SrcWT-paGFP</t> ( second and fourth rows ) in the absence ( first and second rows ) or presence ( third and fourth rows ) of <t>UNC119A-TagBFP</t> ectopic expression. Src-paGFP was photoactivated at 0 s in the perinuclear region and subsequently Src-mCh was photobleached at 400 s in the same area (photoactivation/photobleach area: red outline ). b Segmentation of cells in ( a ): RE ( red ), PM ( blue outline ) and communicating cytoplasm ( green ). The ratio of Src-paGFP fluorescence per segment (RE, red ; PM, blue ; cytoplasm, green ) over total fluorescence in the cell and normalised to the respective ratio of Src-mCh fluorescence was plotted against time. The PM ratio was fitted to a mono-exponential recovery ( black line ). c Average traces of FLAP and FRAP curves fitted to the compartmental model described in Methods. Loss of Src-paGFP fluorescence at the perinuclear region normalised to whole-cell Src-paGFP fluorescence ( blue and cyan curves ) and corresponding gain of Src-mCh fluorescence after photobleaching at the same ROI, normalised to whole-cell Src-mCh fluorescence ( red and orange curves ). Control cells: mean ± SEM; n = 5 cells ( blue and red curves ), UNC119 ectopic expression: mean ± SEM; n = 6 cells ( cyan and orange curves ). Scale bars , 10 μm
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    Src released from UNC119 by Arl2/3 is trapped at the RE to be transported to the PM. a Representative time-lapse sequences of fluorescence distribution of HeLa cells co-expressing Src-mCh ( first and third rows ) and <t>SrcWT-paGFP</t> ( second and fourth rows ) in the absence ( first and second rows ) or presence ( third and fourth rows ) of <t>UNC119A-TagBFP</t> ectopic expression. Src-paGFP was photoactivated at 0 s in the perinuclear region and subsequently Src-mCh was photobleached at 400 s in the same area (photoactivation/photobleach area: red outline ). b Segmentation of cells in ( a ): RE ( red ), PM ( blue outline ) and communicating cytoplasm ( green ). The ratio of Src-paGFP fluorescence per segment (RE, red ; PM, blue ; cytoplasm, green ) over total fluorescence in the cell and normalised to the respective ratio of Src-mCh fluorescence was plotted against time. The PM ratio was fitted to a mono-exponential recovery ( black line ). c Average traces of FLAP and FRAP curves fitted to the compartmental model described in Methods. Loss of Src-paGFP fluorescence at the perinuclear region normalised to whole-cell Src-paGFP fluorescence ( blue and cyan curves ) and corresponding gain of Src-mCh fluorescence after photobleaching at the same ROI, normalised to whole-cell Src-mCh fluorescence ( red and orange curves ). Control cells: mean ± SEM; n = 5 cells ( blue and red curves ), UNC119 ectopic expression: mean ± SEM; n = 6 cells ( cyan and orange curves ). Scale bars , 10 μm
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    Image Search Results


    Src released from UNC119 by Arl2/3 is trapped at the RE to be transported to the PM. a Representative time-lapse sequences of fluorescence distribution of HeLa cells co-expressing Src-mCh ( first and third rows ) and SrcWT-paGFP ( second and fourth rows ) in the absence ( first and second rows ) or presence ( third and fourth rows ) of UNC119A-TagBFP ectopic expression. Src-paGFP was photoactivated at 0 s in the perinuclear region and subsequently Src-mCh was photobleached at 400 s in the same area (photoactivation/photobleach area: red outline ). b Segmentation of cells in ( a ): RE ( red ), PM ( blue outline ) and communicating cytoplasm ( green ). The ratio of Src-paGFP fluorescence per segment (RE, red ; PM, blue ; cytoplasm, green ) over total fluorescence in the cell and normalised to the respective ratio of Src-mCh fluorescence was plotted against time. The PM ratio was fitted to a mono-exponential recovery ( black line ). c Average traces of FLAP and FRAP curves fitted to the compartmental model described in Methods. Loss of Src-paGFP fluorescence at the perinuclear region normalised to whole-cell Src-paGFP fluorescence ( blue and cyan curves ) and corresponding gain of Src-mCh fluorescence after photobleaching at the same ROI, normalised to whole-cell Src-mCh fluorescence ( red and orange curves ). Control cells: mean ± SEM; n = 5 cells ( blue and red curves ), UNC119 ectopic expression: mean ± SEM; n = 6 cells ( cyan and orange curves ). Scale bars , 10 μm

    Journal: Nature Communications

    Article Title: Spatial cycles mediated by UNC119 solubilisation maintain Src family kinases plasma membrane localisation

    doi: 10.1038/s41467-017-00116-3

    Figure Lengend Snippet: Src released from UNC119 by Arl2/3 is trapped at the RE to be transported to the PM. a Representative time-lapse sequences of fluorescence distribution of HeLa cells co-expressing Src-mCh ( first and third rows ) and SrcWT-paGFP ( second and fourth rows ) in the absence ( first and second rows ) or presence ( third and fourth rows ) of UNC119A-TagBFP ectopic expression. Src-paGFP was photoactivated at 0 s in the perinuclear region and subsequently Src-mCh was photobleached at 400 s in the same area (photoactivation/photobleach area: red outline ). b Segmentation of cells in ( a ): RE ( red ), PM ( blue outline ) and communicating cytoplasm ( green ). The ratio of Src-paGFP fluorescence per segment (RE, red ; PM, blue ; cytoplasm, green ) over total fluorescence in the cell and normalised to the respective ratio of Src-mCh fluorescence was plotted against time. The PM ratio was fitted to a mono-exponential recovery ( black line ). c Average traces of FLAP and FRAP curves fitted to the compartmental model described in Methods. Loss of Src-paGFP fluorescence at the perinuclear region normalised to whole-cell Src-paGFP fluorescence ( blue and cyan curves ) and corresponding gain of Src-mCh fluorescence after photobleaching at the same ROI, normalised to whole-cell Src-mCh fluorescence ( red and orange curves ). Control cells: mean ± SEM; n = 5 cells ( blue and red curves ), UNC119 ectopic expression: mean ± SEM; n = 6 cells ( cyan and orange curves ). Scale bars , 10 μm

    Article Snippet: All PCR-derived sequences in the final constructs were verified by DNA sequencing. mCitrine-N1/C1, mCherry-N1/C1, mTFP-N1, TagBFP-N1 and PAGFP-N1 were generated by insertion of AgeI/BsrGI PCR fragments of mCitrine, mCherry, mTFP (gifts from R Tsien), TagBFP (Evrogen) and PAGFP (gift from J Lippincott-Schwartz) complementary DNA (cDNA) into pEGFP-N1 or pEGFP-C1 (Clontech, Saint-Germain-en-Laye, France). cDNA clones encoding full-length UNC119A (accession No: NM_005148), UNC119B (accession No: NM_001080533), C-SRC (accession No: NM_005417), FYN (accession No: NM_002037) and YES1 (accession No: NM_005433) in the pCMV6 vector were obtained by BioCat (BioCat GmbH, Heidelberg, Germany).

    Techniques: Fluorescence, Expressing