affinity chromatography Search Results


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  • 99
    Thermo Fisher affinity chromatography
    Affinity Chromatography, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 818 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore affinity chromatography
    Affinity Chromatography, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 870 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad affinity chromatography
    Affinity Chromatography, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 214 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare affinity chromatography
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    TaKaRa metal affinity chromatography
    Metal Affinity Chromatography, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1577 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare nickel affinity chromatography
    Nickel Affinity Chromatography, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 4801 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare glutathione sepharose 4b affinity chromatography
    Glutathione Sepharose 4b Affinity Chromatography, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 280 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore nickel affinity chromatography
    Nickel Affinity Chromatography, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1046 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher poros 20 mc metal chelate affinity resin
    Poros 20 Mc Metal Chelate Affinity Resin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MACHEREY NAGEL affinity chromatography
    Affinity Chromatography, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 92/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher protein g affinity chromatography
    Protein G Affinity Chromatography, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 220 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher protein a affinity chromatography
    Protein A Affinity Chromatography, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 277 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen nickel affinity chromatography
    Nickel Affinity Chromatography, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2570 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher metal affinity chromatography
    Metal Affinity Chromatography, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 697 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher nickel affinity chromatography
    Nickel Affinity Chromatography, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 482 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore imac select affinity gel
    Overexpression of Sks1 in yeast cells increases Ser57 phosphorylation of ubiquitin. Cells were grown in SILAC media supplemented with light or heavy lysine and arginine. Chromosomally expressed 3xFLAG-tagged ubiquitin (from the RPS31 and RPL40B loci) was isolated by affinity purification and digested with trypsin. <t>Phosphopeptides</t> were enriched by immobilized metal affinity chromatography <t>(IMAC),</t> separated by a capillary reverse phase analytical column, and analyzed on a Q Exactive mass spectrometer. Theoretical masses of b/y ions are tabulated and MS-observed masses are shown in the spectra.
    Imac Select Affinity Gel, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher ni nta affinity chromatography
    Overexpression of Sks1 in yeast cells increases Ser57 phosphorylation of ubiquitin. Cells were grown in SILAC media supplemented with light or heavy lysine and arginine. Chromosomally expressed 3xFLAG-tagged ubiquitin (from the RPS31 and RPL40B loci) was isolated by affinity purification and digested with trypsin. <t>Phosphopeptides</t> were enriched by immobilized metal affinity chromatography <t>(IMAC),</t> separated by a capillary reverse phase analytical column, and analyzed on a Q Exactive mass spectrometer. Theoretical masses of b/y ions are tabulated and MS-observed masses are shown in the spectra.
    Ni Nta Affinity Chromatography, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 235 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher probond nickel chelating resin
    Overexpression of Sks1 in yeast cells increases Ser57 phosphorylation of ubiquitin. Cells were grown in SILAC media supplemented with light or heavy lysine and arginine. Chromosomally expressed 3xFLAG-tagged ubiquitin (from the RPS31 and RPL40B loci) was isolated by affinity purification and digested with trypsin. <t>Phosphopeptides</t> were enriched by immobilized metal affinity chromatography <t>(IMAC),</t> separated by a capillary reverse phase analytical column, and analyzed on a Q Exactive mass spectrometer. Theoretical masses of b/y ions are tabulated and MS-observed masses are shown in the spectra.
    Probond Nickel Chelating Resin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 752 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    IBA GmbH affinity chromatography
    Overexpression of Sks1 in yeast cells increases Ser57 phosphorylation of ubiquitin. Cells were grown in SILAC media supplemented with light or heavy lysine and arginine. Chromosomally expressed 3xFLAG-tagged ubiquitin (from the RPS31 and RPL40B loci) was isolated by affinity purification and digested with trypsin. <t>Phosphopeptides</t> were enriched by immobilized metal affinity chromatography <t>(IMAC),</t> separated by a capillary reverse phase analytical column, and analyzed on a Q Exactive mass spectrometer. Theoretical masses of b/y ions are tabulated and MS-observed masses are shown in the spectra.
    Affinity Chromatography, supplied by IBA GmbH, used in various techniques. Bioz Stars score: 92/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad protein a affinity chromatography
    SPR analyses of sCD4 interactions with human anti-gp120 MAbs. Threefold serial concentrations of sCD4 were flowed over human anti-gp120 MAbs that were immobilized on a protein A-coated CM4 chip. MAb 19e (filled circles), MAb 21c (open circles), and MAb
    Protein A Affinity Chromatography, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 234 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen nickel nitrilotriacetic acid ni nta affinity chromatography
    SPR analyses of sCD4 interactions with human anti-gp120 MAbs. Threefold serial concentrations of sCD4 were flowed over human anti-gp120 MAbs that were immobilized on a protein A-coated CM4 chip. MAb 19e (filled circles), MAb 21c (open circles), and MAb
    Nickel Nitrilotriacetic Acid Ni Nta Affinity Chromatography, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 342 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GenScript affinity chromatography
    SPR analyses of sCD4 interactions with human anti-gp120 MAbs. Threefold serial concentrations of sCD4 were flowed over human anti-gp120 MAbs that were immobilized on a protein A-coated CM4 chip. MAb 19e (filled circles), MAb 21c (open circles), and MAb
    Affinity Chromatography, supplied by GenScript, used in various techniques. Bioz Stars score: 90/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad metal affinity chromatography
    SPR analyses of sCD4 interactions with human anti-gp120 MAbs. Threefold serial concentrations of sCD4 were flowed over human anti-gp120 MAbs that were immobilized on a protein A-coated CM4 chip. MAb 19e (filled circles), MAb 21c (open circles), and MAb
    Metal Affinity Chromatography, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 265 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Overexpression of Sks1 in yeast cells increases Ser57 phosphorylation of ubiquitin. Cells were grown in SILAC media supplemented with light or heavy lysine and arginine. Chromosomally expressed 3xFLAG-tagged ubiquitin (from the RPS31 and RPL40B loci) was isolated by affinity purification and digested with trypsin. Phosphopeptides were enriched by immobilized metal affinity chromatography (IMAC), separated by a capillary reverse phase analytical column, and analyzed on a Q Exactive mass spectrometer. Theoretical masses of b/y ions are tabulated and MS-observed masses are shown in the spectra.

    Journal: bioRxiv

    Article Title: Identification of ubiquitin Ser57 kinases regulating the oxidative stress response in yeast

    doi: 10.1101/2020.06.20.162883

    Figure Lengend Snippet: Overexpression of Sks1 in yeast cells increases Ser57 phosphorylation of ubiquitin. Cells were grown in SILAC media supplemented with light or heavy lysine and arginine. Chromosomally expressed 3xFLAG-tagged ubiquitin (from the RPS31 and RPL40B loci) was isolated by affinity purification and digested with trypsin. Phosphopeptides were enriched by immobilized metal affinity chromatography (IMAC), separated by a capillary reverse phase analytical column, and analyzed on a Q Exactive mass spectrometer. Theoretical masses of b/y ions are tabulated and MS-observed masses are shown in the spectra.

    Article Snippet: Phosphopeptides were enriched by immobilized metal affinity chromatography (IMAC) using Fe(III)-nitrilotriacetic acid resin as previously describedand dissolved in 0.1% trifluoroacetic acid.

    Techniques: Over Expression, Isolation, Affinity Purification, Affinity Chromatography, Mass Spectrometry

    SILAC-MS fragmentation spectra of unmodified (top) and Ser57-phosphorylated (bottom) peptides of ubiquitin isolated from yeast cells grown in the presence (light) or absence (heavy) of H 2 O 2 . Cells were grown in SILAC media (supplemented with light or heavy lysine and arginine) to mid-log phase (OD 600 of 0.6-0.7) and treated with 0.6 mM H 2 O 2 for 30 minutes. Chromosomally expressed 3xFLAG-tagged ubiquitin (from the RPS31 and RPL40B loci) was isolated by affinity purification and digested with trypsin. Phosphopeptides were enriched by immobilized metal affinity chromatography (IMAC), separated by a capillary reverse phase analytical column, and analyzed on a Q Exactive mass spectrometer. Theoretical masses of b/y ions are tabulated and MS-observed masses are shown in the spectra.

    Journal: bioRxiv

    Article Title: Identification of ubiquitin Ser57 kinases regulating the oxidative stress response in yeast

    doi: 10.1101/2020.06.20.162883

    Figure Lengend Snippet: SILAC-MS fragmentation spectra of unmodified (top) and Ser57-phosphorylated (bottom) peptides of ubiquitin isolated from yeast cells grown in the presence (light) or absence (heavy) of H 2 O 2 . Cells were grown in SILAC media (supplemented with light or heavy lysine and arginine) to mid-log phase (OD 600 of 0.6-0.7) and treated with 0.6 mM H 2 O 2 for 30 minutes. Chromosomally expressed 3xFLAG-tagged ubiquitin (from the RPS31 and RPL40B loci) was isolated by affinity purification and digested with trypsin. Phosphopeptides were enriched by immobilized metal affinity chromatography (IMAC), separated by a capillary reverse phase analytical column, and analyzed on a Q Exactive mass spectrometer. Theoretical masses of b/y ions are tabulated and MS-observed masses are shown in the spectra.

    Article Snippet: Phosphopeptides were enriched by immobilized metal affinity chromatography (IMAC) using Fe(III)-nitrilotriacetic acid resin as previously describedand dissolved in 0.1% trifluoroacetic acid.

    Techniques: Isolation, Affinity Purification, Affinity Chromatography, Mass Spectrometry

    SPR analyses of sCD4 interactions with human anti-gp120 MAbs. Threefold serial concentrations of sCD4 were flowed over human anti-gp120 MAbs that were immobilized on a protein A-coated CM4 chip. MAb 19e (filled circles), MAb 21c (open circles), and MAb

    Journal: Journal of Virology

    Article Title: Identification and Characterization of an Immunogenic Hybrid Epitope Formed by both HIV gp120 and Human CD4 Proteins ▿

    doi: 10.1128/JVI.05072-11

    Figure Lengend Snippet: SPR analyses of sCD4 interactions with human anti-gp120 MAbs. Threefold serial concentrations of sCD4 were flowed over human anti-gp120 MAbs that were immobilized on a protein A-coated CM4 chip. MAb 19e (filled circles), MAb 21c (open circles), and MAb

    Article Snippet: Antibodies were purified from culture supernatants by protein A affinity chromatography, dialyzed against phosphate-buffered saline (PBS), and quantified by bicinchoninic acid (BCA) assay (Bio-Rad, Hercules, CA) according to the manufacturer's protocol.

    Techniques: SPR Assay, Chromatin Immunoprecipitation