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  • 94
    TaKaRa advantage hd dna polymerase mix
    Lhx2 binds to the promoter regions of the Sox9 , <t>Tcf4</t> and Lgr5 genes in keratinocytes. Mouse keratinocytes isolated from adult telogen skin were processed for ChIP-qPCR analysis with an antibody against Lhx2 or purified goat IgG. HaCaT keratinocytes were processed for detection of the Sox9 and Tcf4 promoter activities. ( A ) Sox9, Tcf4 and Lgr5 promoter regions with putative Lim-homeodomain <t>DNA-binding</t> sites or control sites predicted by bioinformatic analysis. ( B ) ChIP-qPCR analysis of the distinct regions in the Sox9, Tcf4 and Lgr5 promoters, showing enrichment with Lhx2 antibody compared with IgG controls (mean±s.e.m., n =3; * P
    Advantage Hd Dna Polymerase Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 275 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 275 article reviews
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    90
    TaKaRa advantage ultrapure pcr deoxynucleotide mix
    Lhx2 binds to the promoter regions of the Sox9 , <t>Tcf4</t> and Lgr5 genes in keratinocytes. Mouse keratinocytes isolated from adult telogen skin were processed for ChIP-qPCR analysis with an antibody against Lhx2 or purified goat IgG. HaCaT keratinocytes were processed for detection of the Sox9 and Tcf4 promoter activities. ( A ) Sox9, Tcf4 and Lgr5 promoter regions with putative Lim-homeodomain <t>DNA-binding</t> sites or control sites predicted by bioinformatic analysis. ( B ) ChIP-qPCR analysis of the distinct regions in the Sox9, Tcf4 and Lgr5 promoters, showing enrichment with Lhx2 antibody compared with IgG controls (mean±s.e.m., n =3; * P
    Advantage Ultrapure Pcr Deoxynucleotide Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 500 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/advantage ultrapure pcr deoxynucleotide mix/product/TaKaRa
    Average 90 stars, based on 500 article reviews
    Price from $9.99 to $1999.99
    advantage ultrapure pcr deoxynucleotide mix - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

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    Lhx2 binds to the promoter regions of the Sox9 , Tcf4 and Lgr5 genes in keratinocytes. Mouse keratinocytes isolated from adult telogen skin were processed for ChIP-qPCR analysis with an antibody against Lhx2 or purified goat IgG. HaCaT keratinocytes were processed for detection of the Sox9 and Tcf4 promoter activities. ( A ) Sox9, Tcf4 and Lgr5 promoter regions with putative Lim-homeodomain DNA-binding sites or control sites predicted by bioinformatic analysis. ( B ) ChIP-qPCR analysis of the distinct regions in the Sox9, Tcf4 and Lgr5 promoters, showing enrichment with Lhx2 antibody compared with IgG controls (mean±s.e.m., n =3; * P

    Journal: Development (Cambridge, England)

    Article Title: Lhx2 differentially regulates Sox9, Tcf4 and Lgr5 in hair follicle stem cells to promote epidermal regeneration after injury

    doi: 10.1242/dev.070284

    Figure Lengend Snippet: Lhx2 binds to the promoter regions of the Sox9 , Tcf4 and Lgr5 genes in keratinocytes. Mouse keratinocytes isolated from adult telogen skin were processed for ChIP-qPCR analysis with an antibody against Lhx2 or purified goat IgG. HaCaT keratinocytes were processed for detection of the Sox9 and Tcf4 promoter activities. ( A ) Sox9, Tcf4 and Lgr5 promoter regions with putative Lim-homeodomain DNA-binding sites or control sites predicted by bioinformatic analysis. ( B ) ChIP-qPCR analysis of the distinct regions in the Sox9, Tcf4 and Lgr5 promoters, showing enrichment with Lhx2 antibody compared with IgG controls (mean±s.e.m., n =3; * P

    Article Snippet: To generate a Tcf4 reporter construct, the Tcf4 promoter region (–3598/+462) was PCR amplified from mouse genomic DNA with Advantage HD DNA polymerase (Clontech) using the following primers ( Bgl II and Hin dIII restriction sites are underlined): 5′-CCCGGGCTCG AGATCT TGACCACACCCCCACCACTT-3′ (F) and 5′-CCGGAATGCC AAGCTT GCATTTTTCACCCACCAGCAGC-3′ (R).

    Techniques: Isolation, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction, Purification, Binding Assay