adaptor-ligation Search Results


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  • 99
    New England Biolabs adaptor ligation
    Adaptor Ligation, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1455 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adaptor ligation/product/New England Biolabs
    Average 99 stars, based on 1455 article reviews
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    99
    Millipore adaptor ligated dna
    Adaptor Ligated Dna, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adaptor ligated dna/product/Millipore
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    94
    Illumina Inc adaptor ligation
    <t>Illumina</t> sequencing. (A) Library construction after ribosomal RNA depletion using adaptors for the Illumina platform. (B) A graph showing the degree of colinearity between gene expression levels measured by qRT-PCR and by deep sequencing for our set of
    Adaptor Ligation, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 2297 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adaptor ligation/product/Illumina Inc
    Average 94 stars, based on 2297 article reviews
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    88
    Illumina Inc adaptor ligated library
    <t>Illumina</t> sequencing. (A) Library construction after ribosomal RNA depletion using adaptors for the Illumina platform. (B) A graph showing the degree of colinearity between gene expression levels measured by qRT-PCR and by deep sequencing for our set of
    Adaptor Ligated Library, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adaptor ligated library/product/Illumina Inc
    Average 88 stars, based on 63 article reviews
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    adaptor ligated library - by Bioz Stars, 2020-09
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    92
    Illumina Inc adaptor ligated dna
    <t>Illumina</t> sequencing. (A) Library construction after ribosomal RNA depletion using adaptors for the Illumina platform. (B) A graph showing the degree of colinearity between gene expression levels measured by qRT-PCR and by deep sequencing for our set of
    Adaptor Ligated Dna, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 147 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adaptor ligated dna/product/Illumina Inc
    Average 92 stars, based on 147 article reviews
    Price from $9.99 to $1999.99
    adaptor ligated dna - by Bioz Stars, 2020-09
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    92
    Solexa adaptor ligation
    <t>Illumina</t> sequencing. (A) Library construction after ribosomal RNA depletion using adaptors for the Illumina platform. (B) A graph showing the degree of colinearity between gene expression levels measured by qRT-PCR and by deep sequencing for our set of
    Adaptor Ligation, supplied by Solexa, used in various techniques. Bioz Stars score: 92/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adaptor ligation/product/Solexa
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    adaptor ligation - by Bioz Stars, 2020-09
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    85
    Illumina Inc standard illumina adaptor ligation protocol
    <t>Illumina</t> sequencing. (A) Library construction after ribosomal RNA depletion using adaptors for the Illumina platform. (B) A graph showing the degree of colinearity between gene expression levels measured by qRT-PCR and by deep sequencing for our set of
    Standard Illumina Adaptor Ligation Protocol, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 85/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/standard illumina adaptor ligation protocol/product/Illumina Inc
    Average 85 stars, based on 34 article reviews
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    93
    Thermo Fisher adaptor ligation
    <t>Illumina</t> sequencing. (A) Library construction after ribosomal RNA depletion using adaptors for the Illumina platform. (B) A graph showing the degree of colinearity between gene expression levels measured by qRT-PCR and by deep sequencing for our set of
    Adaptor Ligation, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 574 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Pacific Biosciences adaptor ligation
    <t>Illumina</t> sequencing. (A) Library construction after ribosomal RNA depletion using adaptors for the Illumina platform. (B) A graph showing the degree of colinearity between gene expression levels measured by qRT-PCR and by deep sequencing for our set of
    Adaptor Ligation, supplied by Pacific Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adaptor ligation/product/Pacific Biosciences
    Average 93 stars, based on 30 article reviews
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    88
    Illumina Inc sequencing adaptor ligation
    <t>Illumina</t> sequencing. (A) Library construction after ribosomal RNA depletion using adaptors for the Illumina platform. (B) A graph showing the degree of colinearity between gene expression levels measured by qRT-PCR and by deep sequencing for our set of
    Sequencing Adaptor Ligation, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequencing adaptor ligation/product/Illumina Inc
    Average 88 stars, based on 26 article reviews
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    sequencing adaptor ligation - by Bioz Stars, 2020-09
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    99
    New England Biolabs nebnext ultra ii dna library prep kit for illumina
    <t>Illumina</t> sequencing. (A) Library construction after ribosomal RNA depletion using adaptors for the Illumina platform. (B) A graph showing the degree of colinearity between gene expression levels measured by qRT-PCR and by deep sequencing for our set of
    Nebnext Ultra Ii Dna Library Prep Kit For Illumina, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1478 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext ultra ii dna library prep kit for illumina/product/New England Biolabs
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    89
    Illumina Inc rna seq adaptor ligation reactions
    <t>Illumina</t> sequencing. (A) Library construction after ribosomal RNA depletion using adaptors for the Illumina platform. (B) A graph showing the degree of colinearity between gene expression levels measured by qRT-PCR and by deep sequencing for our set of
    Rna Seq Adaptor Ligation Reactions, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 89/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Pacific Biosciences smrtbell adaptor ligation step
    Illustration of PacBio sequence generation. Adaptors (SMRTbells) are first ligated to each amplicon, and after a sequencing primer is annealed to the <t>SMRTbell</t> template, DNA polymerase is bound to the complex. This polymerase-amplicon-adaptor complex is then loaded into zero-mode waveguides (ZMWs) where replication occurs, producing nucleotide-specific fluorescence. Circular consensus sequencing (ccs) allows the polymerase to repeatedly replicate the circularized strand, producing one long read with randomly distributed errors [ 9 ]. Post-run, the SMRTbell sequences are bioinformatically trimmed away, single-molecule fragments are aligned, and a consensus sequence is generated. The single-molecule coverage and accuracy of resulting ccs reads are amplicon- and read-length dependent, with smaller amplicons and longer reads giving higher single-molecule coverage and thus higher ccs read accuracy.
    Smrtbell Adaptor Ligation Step, supplied by Pacific Biosciences, used in various techniques. Bioz Stars score: 85/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/smrtbell adaptor ligation step/product/Pacific Biosciences
    Average 85 stars, based on 16 article reviews
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    smrtbell adaptor ligation step - by Bioz Stars, 2020-09
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    92
    Promega adaptor ligation
    Illustration of PacBio sequence generation. Adaptors (SMRTbells) are first ligated to each amplicon, and after a sequencing primer is annealed to the <t>SMRTbell</t> template, DNA polymerase is bound to the complex. This polymerase-amplicon-adaptor complex is then loaded into zero-mode waveguides (ZMWs) where replication occurs, producing nucleotide-specific fluorescence. Circular consensus sequencing (ccs) allows the polymerase to repeatedly replicate the circularized strand, producing one long read with randomly distributed errors [ 9 ]. Post-run, the SMRTbell sequences are bioinformatically trimmed away, single-molecule fragments are aligned, and a consensus sequence is generated. The single-molecule coverage and accuracy of resulting ccs reads are amplicon- and read-length dependent, with smaller amplicons and longer reads giving higher single-molecule coverage and thus higher ccs read accuracy.
    Adaptor Ligation, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adaptor ligation/product/Promega
    Average 92 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    adaptor ligation - by Bioz Stars, 2020-09
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    99
    Millipore adaptor ligated gdna fragments
    Illustration of PacBio sequence generation. Adaptors (SMRTbells) are first ligated to each amplicon, and after a sequencing primer is annealed to the <t>SMRTbell</t> template, DNA polymerase is bound to the complex. This polymerase-amplicon-adaptor complex is then loaded into zero-mode waveguides (ZMWs) where replication occurs, producing nucleotide-specific fluorescence. Circular consensus sequencing (ccs) allows the polymerase to repeatedly replicate the circularized strand, producing one long read with randomly distributed errors [ 9 ]. Post-run, the SMRTbell sequences are bioinformatically trimmed away, single-molecule fragments are aligned, and a consensus sequence is generated. The single-molecule coverage and accuracy of resulting ccs reads are amplicon- and read-length dependent, with smaller amplicons and longer reads giving higher single-molecule coverage and thus higher ccs read accuracy.
    Adaptor Ligated Gdna Fragments, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adaptor ligated gdna fragments/product/Millipore
    Average 99 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
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    Image Search Results


    Illumina sequencing. (A) Library construction after ribosomal RNA depletion using adaptors for the Illumina platform. (B) A graph showing the degree of colinearity between gene expression levels measured by qRT-PCR and by deep sequencing for our set of

    Journal: Stem Cells and Development

    Article Title: Widespread Transcription in an Amphibian Oocyte Relates to Its Reprogramming Activity on Transplanted Somatic Nuclei

    doi: 10.1089/scd.2011.0162

    Figure Lengend Snippet: Illumina sequencing. (A) Library construction after ribosomal RNA depletion using adaptors for the Illumina platform. (B) A graph showing the degree of colinearity between gene expression levels measured by qRT-PCR and by deep sequencing for our set of

    Article Snippet: End repair and 3′ adenylation were performed before Illumina adaptor ligation.

    Techniques: Sequencing, Expressing, Quantitative RT-PCR

    Illustration of PacBio sequence generation. Adaptors (SMRTbells) are first ligated to each amplicon, and after a sequencing primer is annealed to the SMRTbell template, DNA polymerase is bound to the complex. This polymerase-amplicon-adaptor complex is then loaded into zero-mode waveguides (ZMWs) where replication occurs, producing nucleotide-specific fluorescence. Circular consensus sequencing (ccs) allows the polymerase to repeatedly replicate the circularized strand, producing one long read with randomly distributed errors [ 9 ]. Post-run, the SMRTbell sequences are bioinformatically trimmed away, single-molecule fragments are aligned, and a consensus sequence is generated. The single-molecule coverage and accuracy of resulting ccs reads are amplicon- and read-length dependent, with smaller amplicons and longer reads giving higher single-molecule coverage and thus higher ccs read accuracy.

    Journal: Microbiome

    Article Title: Microbial phylogenetic profiling with the Pacific Biosciences sequencing platform

    doi: 10.1186/2049-2618-1-10

    Figure Lengend Snippet: Illustration of PacBio sequence generation. Adaptors (SMRTbells) are first ligated to each amplicon, and after a sequencing primer is annealed to the SMRTbell template, DNA polymerase is bound to the complex. This polymerase-amplicon-adaptor complex is then loaded into zero-mode waveguides (ZMWs) where replication occurs, producing nucleotide-specific fluorescence. Circular consensus sequencing (ccs) allows the polymerase to repeatedly replicate the circularized strand, producing one long read with randomly distributed errors [ 9 ]. Post-run, the SMRTbell sequences are bioinformatically trimmed away, single-molecule fragments are aligned, and a consensus sequence is generated. The single-molecule coverage and accuracy of resulting ccs reads are amplicon- and read-length dependent, with smaller amplicons and longer reads giving higher single-molecule coverage and thus higher ccs read accuracy.

    Article Snippet: Unlike PCR-generated chimeras, the heterologous fragments of these chimeras were full-length amplicons, complete with primer and barcode sequences, leading to the hypothesis that these chimeras were generated during the SMRTbell adaptor ligation step of PacBio library preparation (Figure ).

    Techniques: Sequencing, Amplification, Fluorescence, Generated