adaptor-ligated dnas Search Results


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  • 99
    New England Biolabs nebnext ultra ii dna library prep kit for illumina
    Nebnext Ultra Ii Dna Library Prep Kit For Illumina, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1478 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext ultra ii dna library prep kit for illumina/product/New England Biolabs
    Average 99 stars, based on 1478 article reviews
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    nebnext ultra ii dna library prep kit for illumina - by Bioz Stars, 2020-09
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    99
    Millipore adaptor ligated dna
    Adaptor Ligated Dna, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Illumina Inc adaptor ligated dna
    The identity and distribution of <t>DNA</t> nucleotide mismatches in individual <t>Illumina</t> GA reads compared to the CPC98 consensus mtDNA genome. (A) The number and proportion of each nucleotide called in the Illumina GA reads (vertical column) compared to the consensus mtDNA sequence (horizontal column) is presented. (B) Mean percentage of discordant nucleotides for each position across all individual Illumina GA sequence reads.
    Adaptor Ligated Dna, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 152 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore adaptor ligated gdna fragments
    The identity and distribution of <t>DNA</t> nucleotide mismatches in individual <t>Illumina</t> GA reads compared to the CPC98 consensus mtDNA genome. (A) The number and proportion of each nucleotide called in the Illumina GA reads (vertical column) compared to the consensus mtDNA sequence (horizontal column) is presented. (B) Mean percentage of discordant nucleotides for each position across all individual Illumina GA sequence reads.
    Adaptor Ligated Gdna Fragments, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    New England Biolabs eco p15i adaptor ligated gel purified dna
    Cloning of a miRNA sgRNA library using the Molecular Chipper method. ( a ) Overview of the Molecular Chipper method to generate a sgRNA library from pieces of input <t>DNA.</t> ( b ) Detailed schematics of the Molecular Chipper procedure. Briefly, an <t>EcoP15I-site-containing</t> adaptor is ligated to randomly fragmented DNA ends, and enzymatically released 20 bases (a G base plus 19 bases from ends of DNA fragments) are cloned as a pool into a viral vector. ( c ) Seventeen murine miRNAs (or miRNA cluster) and their flanking genomic sequences were used to generate a sgRNA library. Length distribution of the targeting portions of sgRNAs within the library is shown. Note that the length was calculated by one base G (in adaptor) plus the length of random ends of fragments from input DNA. The counts for each length are normalized to those of the 20-base-targeting motif sgRNAs within each biological replicate. Error bars represent s.d. N =3 biological replicates. ( d ) The distributions of the distances between neighbouring sgRNAs with NGG-PAM, based on all sgRNAs detected in deep sequencing, are shown (red line). The median neighbour distance is 8 bp. Theoretical distribution assumes all possible NGG-PAM sgRNAs (blue line) are present. ( e ) Top: diagram showing that the 17 murine miRNAs (or miRNA cluster) and their flanking genomic sequences were used to generate a sgRNA library. Bottom: representative graphs of sgRNA counts mapping to the miR-142 region or to the miR-126 region from one out of three neg-GFP samples is shown, with blue and red indicating mapping to sense and antisense strands, respectively. The positions of sgRNAs plotted were only based on positions of the last targeting domain base.
    Eco P15i Adaptor Ligated Gel Purified Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eco p15i adaptor ligated gel purified dna/product/New England Biolabs
    Average 95 stars, based on 14 article reviews
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    99
    New England Biolabs adaptor ligation nebnext dna library prep modules for illumina
    Cloning of a miRNA sgRNA library using the Molecular Chipper method. ( a ) Overview of the Molecular Chipper method to generate a sgRNA library from pieces of input <t>DNA.</t> ( b ) Detailed schematics of the Molecular Chipper procedure. Briefly, an <t>EcoP15I-site-containing</t> adaptor is ligated to randomly fragmented DNA ends, and enzymatically released 20 bases (a G base plus 19 bases from ends of DNA fragments) are cloned as a pool into a viral vector. ( c ) Seventeen murine miRNAs (or miRNA cluster) and their flanking genomic sequences were used to generate a sgRNA library. Length distribution of the targeting portions of sgRNAs within the library is shown. Note that the length was calculated by one base G (in adaptor) plus the length of random ends of fragments from input DNA. The counts for each length are normalized to those of the 20-base-targeting motif sgRNAs within each biological replicate. Error bars represent s.d. N =3 biological replicates. ( d ) The distributions of the distances between neighbouring sgRNAs with NGG-PAM, based on all sgRNAs detected in deep sequencing, are shown (red line). The median neighbour distance is 8 bp. Theoretical distribution assumes all possible NGG-PAM sgRNAs (blue line) are present. ( e ) Top: diagram showing that the 17 murine miRNAs (or miRNA cluster) and their flanking genomic sequences were used to generate a sgRNA library. Bottom: representative graphs of sgRNA counts mapping to the miR-142 region or to the miR-126 region from one out of three neg-GFP samples is shown, with blue and red indicating mapping to sense and antisense strands, respectively. The positions of sgRNAs plotted were only based on positions of the last targeting domain base.
    Adaptor Ligation Nebnext Dna Library Prep Modules For Illumina, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adaptor ligation nebnext dna library prep modules for illumina/product/New England Biolabs
    Average 99 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    adaptor ligation nebnext dna library prep modules for illumina - by Bioz Stars, 2020-09
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    99
    New England Biolabs adaptor ligation
    Cloning of a miRNA sgRNA library using the Molecular Chipper method. ( a ) Overview of the Molecular Chipper method to generate a sgRNA library from pieces of input <t>DNA.</t> ( b ) Detailed schematics of the Molecular Chipper procedure. Briefly, an <t>EcoP15I-site-containing</t> adaptor is ligated to randomly fragmented DNA ends, and enzymatically released 20 bases (a G base plus 19 bases from ends of DNA fragments) are cloned as a pool into a viral vector. ( c ) Seventeen murine miRNAs (or miRNA cluster) and their flanking genomic sequences were used to generate a sgRNA library. Length distribution of the targeting portions of sgRNAs within the library is shown. Note that the length was calculated by one base G (in adaptor) plus the length of random ends of fragments from input DNA. The counts for each length are normalized to those of the 20-base-targeting motif sgRNAs within each biological replicate. Error bars represent s.d. N =3 biological replicates. ( d ) The distributions of the distances between neighbouring sgRNAs with NGG-PAM, based on all sgRNAs detected in deep sequencing, are shown (red line). The median neighbour distance is 8 bp. Theoretical distribution assumes all possible NGG-PAM sgRNAs (blue line) are present. ( e ) Top: diagram showing that the 17 murine miRNAs (or miRNA cluster) and their flanking genomic sequences were used to generate a sgRNA library. Bottom: representative graphs of sgRNA counts mapping to the miR-142 region or to the miR-126 region from one out of three neg-GFP samples is shown, with blue and red indicating mapping to sense and antisense strands, respectively. The positions of sgRNAs plotted were only based on positions of the last targeting domain base.
    Adaptor Ligation, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1455 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adaptor ligation/product/New England Biolabs
    Average 99 stars, based on 1455 article reviews
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    adaptor ligation - by Bioz Stars, 2020-09
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    99
    TaKaRa adaptor ligated human genomic dna libraries
    Cloning of a miRNA sgRNA library using the Molecular Chipper method. ( a ) Overview of the Molecular Chipper method to generate a sgRNA library from pieces of input <t>DNA.</t> ( b ) Detailed schematics of the Molecular Chipper procedure. Briefly, an <t>EcoP15I-site-containing</t> adaptor is ligated to randomly fragmented DNA ends, and enzymatically released 20 bases (a G base plus 19 bases from ends of DNA fragments) are cloned as a pool into a viral vector. ( c ) Seventeen murine miRNAs (or miRNA cluster) and their flanking genomic sequences were used to generate a sgRNA library. Length distribution of the targeting portions of sgRNAs within the library is shown. Note that the length was calculated by one base G (in adaptor) plus the length of random ends of fragments from input DNA. The counts for each length are normalized to those of the 20-base-targeting motif sgRNAs within each biological replicate. Error bars represent s.d. N =3 biological replicates. ( d ) The distributions of the distances between neighbouring sgRNAs with NGG-PAM, based on all sgRNAs detected in deep sequencing, are shown (red line). The median neighbour distance is 8 bp. Theoretical distribution assumes all possible NGG-PAM sgRNAs (blue line) are present. ( e ) Top: diagram showing that the 17 murine miRNAs (or miRNA cluster) and their flanking genomic sequences were used to generate a sgRNA library. Bottom: representative graphs of sgRNA counts mapping to the miR-142 region or to the miR-126 region from one out of three neg-GFP samples is shown, with blue and red indicating mapping to sense and antisense strands, respectively. The positions of sgRNAs plotted were only based on positions of the last targeting domain base.
    Adaptor Ligated Human Genomic Dna Libraries, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adaptor ligated human genomic dna libraries/product/TaKaRa
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    adaptor ligated human genomic dna libraries - by Bioz Stars, 2020-09
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    90
    TaKaRa adaptor ligated dna fragments
    Cloning of a miRNA sgRNA library using the Molecular Chipper method. ( a ) Overview of the Molecular Chipper method to generate a sgRNA library from pieces of input <t>DNA.</t> ( b ) Detailed schematics of the Molecular Chipper procedure. Briefly, an <t>EcoP15I-site-containing</t> adaptor is ligated to randomly fragmented DNA ends, and enzymatically released 20 bases (a G base plus 19 bases from ends of DNA fragments) are cloned as a pool into a viral vector. ( c ) Seventeen murine miRNAs (or miRNA cluster) and their flanking genomic sequences were used to generate a sgRNA library. Length distribution of the targeting portions of sgRNAs within the library is shown. Note that the length was calculated by one base G (in adaptor) plus the length of random ends of fragments from input DNA. The counts for each length are normalized to those of the 20-base-targeting motif sgRNAs within each biological replicate. Error bars represent s.d. N =3 biological replicates. ( d ) The distributions of the distances between neighbouring sgRNAs with NGG-PAM, based on all sgRNAs detected in deep sequencing, are shown (red line). The median neighbour distance is 8 bp. Theoretical distribution assumes all possible NGG-PAM sgRNAs (blue line) are present. ( e ) Top: diagram showing that the 17 murine miRNAs (or miRNA cluster) and their flanking genomic sequences were used to generate a sgRNA library. Bottom: representative graphs of sgRNA counts mapping to the miR-142 region or to the miR-126 region from one out of three neg-GFP samples is shown, with blue and red indicating mapping to sense and antisense strands, respectively. The positions of sgRNAs plotted were only based on positions of the last targeting domain base.
    Adaptor Ligated Dna Fragments, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    ATUM adaptor ligated dna
    Cloning of a miRNA sgRNA library using the Molecular Chipper method. ( a ) Overview of the Molecular Chipper method to generate a sgRNA library from pieces of input <t>DNA.</t> ( b ) Detailed schematics of the Molecular Chipper procedure. Briefly, an <t>EcoP15I-site-containing</t> adaptor is ligated to randomly fragmented DNA ends, and enzymatically released 20 bases (a G base plus 19 bases from ends of DNA fragments) are cloned as a pool into a viral vector. ( c ) Seventeen murine miRNAs (or miRNA cluster) and their flanking genomic sequences were used to generate a sgRNA library. Length distribution of the targeting portions of sgRNAs within the library is shown. Note that the length was calculated by one base G (in adaptor) plus the length of random ends of fragments from input DNA. The counts for each length are normalized to those of the 20-base-targeting motif sgRNAs within each biological replicate. Error bars represent s.d. N =3 biological replicates. ( d ) The distributions of the distances between neighbouring sgRNAs with NGG-PAM, based on all sgRNAs detected in deep sequencing, are shown (red line). The median neighbour distance is 8 bp. Theoretical distribution assumes all possible NGG-PAM sgRNAs (blue line) are present. ( e ) Top: diagram showing that the 17 murine miRNAs (or miRNA cluster) and their flanking genomic sequences were used to generate a sgRNA library. Bottom: representative graphs of sgRNA counts mapping to the miR-142 region or to the miR-126 region from one out of three neg-GFP samples is shown, with blue and red indicating mapping to sense and antisense strands, respectively. The positions of sgRNAs plotted were only based on positions of the last targeting domain base.
    Adaptor Ligated Dna, supplied by ATUM, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adaptor ligated dna/product/ATUM
    Average 93 stars, based on 6 article reviews
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    adaptor ligated dna - by Bioz Stars, 2020-09
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    92
    Thermo Fisher adaptor ligated dna
    Cloning of a miRNA sgRNA library using the Molecular Chipper method. ( a ) Overview of the Molecular Chipper method to generate a sgRNA library from pieces of input <t>DNA.</t> ( b ) Detailed schematics of the Molecular Chipper procedure. Briefly, an <t>EcoP15I-site-containing</t> adaptor is ligated to randomly fragmented DNA ends, and enzymatically released 20 bases (a G base plus 19 bases from ends of DNA fragments) are cloned as a pool into a viral vector. ( c ) Seventeen murine miRNAs (or miRNA cluster) and their flanking genomic sequences were used to generate a sgRNA library. Length distribution of the targeting portions of sgRNAs within the library is shown. Note that the length was calculated by one base G (in adaptor) plus the length of random ends of fragments from input DNA. The counts for each length are normalized to those of the 20-base-targeting motif sgRNAs within each biological replicate. Error bars represent s.d. N =3 biological replicates. ( d ) The distributions of the distances between neighbouring sgRNAs with NGG-PAM, based on all sgRNAs detected in deep sequencing, are shown (red line). The median neighbour distance is 8 bp. Theoretical distribution assumes all possible NGG-PAM sgRNAs (blue line) are present. ( e ) Top: diagram showing that the 17 murine miRNAs (or miRNA cluster) and their flanking genomic sequences were used to generate a sgRNA library. Bottom: representative graphs of sgRNA counts mapping to the miR-142 region or to the miR-126 region from one out of three neg-GFP samples is shown, with blue and red indicating mapping to sense and antisense strands, respectively. The positions of sgRNAs plotted were only based on positions of the last targeting domain base.
    Adaptor Ligated Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 211 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Illumina Inc dna library construction kit ligates illumina adaptor sequences
    Cloning of a miRNA sgRNA library using the Molecular Chipper method. ( a ) Overview of the Molecular Chipper method to generate a sgRNA library from pieces of input <t>DNA.</t> ( b ) Detailed schematics of the Molecular Chipper procedure. Briefly, an <t>EcoP15I-site-containing</t> adaptor is ligated to randomly fragmented DNA ends, and enzymatically released 20 bases (a G base plus 19 bases from ends of DNA fragments) are cloned as a pool into a viral vector. ( c ) Seventeen murine miRNAs (or miRNA cluster) and their flanking genomic sequences were used to generate a sgRNA library. Length distribution of the targeting portions of sgRNAs within the library is shown. Note that the length was calculated by one base G (in adaptor) plus the length of random ends of fragments from input DNA. The counts for each length are normalized to those of the 20-base-targeting motif sgRNAs within each biological replicate. Error bars represent s.d. N =3 biological replicates. ( d ) The distributions of the distances between neighbouring sgRNAs with NGG-PAM, based on all sgRNAs detected in deep sequencing, are shown (red line). The median neighbour distance is 8 bp. Theoretical distribution assumes all possible NGG-PAM sgRNAs (blue line) are present. ( e ) Top: diagram showing that the 17 murine miRNAs (or miRNA cluster) and their flanking genomic sequences were used to generate a sgRNA library. Bottom: representative graphs of sgRNA counts mapping to the miR-142 region or to the miR-126 region from one out of three neg-GFP samples is shown, with blue and red indicating mapping to sense and antisense strands, respectively. The positions of sgRNAs plotted were only based on positions of the last targeting domain base.
    Dna Library Construction Kit Ligates Illumina Adaptor Sequences, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna library construction kit ligates illumina adaptor sequences/product/Illumina Inc
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    85
    Becton Dickinson uncloned adaptor ligated genomic dna fragments bd genome walker kit bd biosciences
    Cloning of a miRNA sgRNA library using the Molecular Chipper method. ( a ) Overview of the Molecular Chipper method to generate a sgRNA library from pieces of input <t>DNA.</t> ( b ) Detailed schematics of the Molecular Chipper procedure. Briefly, an <t>EcoP15I-site-containing</t> adaptor is ligated to randomly fragmented DNA ends, and enzymatically released 20 bases (a G base plus 19 bases from ends of DNA fragments) are cloned as a pool into a viral vector. ( c ) Seventeen murine miRNAs (or miRNA cluster) and their flanking genomic sequences were used to generate a sgRNA library. Length distribution of the targeting portions of sgRNAs within the library is shown. Note that the length was calculated by one base G (in adaptor) plus the length of random ends of fragments from input DNA. The counts for each length are normalized to those of the 20-base-targeting motif sgRNAs within each biological replicate. Error bars represent s.d. N =3 biological replicates. ( d ) The distributions of the distances between neighbouring sgRNAs with NGG-PAM, based on all sgRNAs detected in deep sequencing, are shown (red line). The median neighbour distance is 8 bp. Theoretical distribution assumes all possible NGG-PAM sgRNAs (blue line) are present. ( e ) Top: diagram showing that the 17 murine miRNAs (or miRNA cluster) and their flanking genomic sequences were used to generate a sgRNA library. Bottom: representative graphs of sgRNA counts mapping to the miR-142 region or to the miR-126 region from one out of three neg-GFP samples is shown, with blue and red indicating mapping to sense and antisense strands, respectively. The positions of sgRNAs plotted were only based on positions of the last targeting domain base.
    Uncloned Adaptor Ligated Genomic Dna Fragments Bd Genome Walker Kit Bd Biosciences, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/uncloned adaptor ligated genomic dna fragments bd genome walker kit bd biosciences/product/Becton Dickinson
    Average 85 stars, based on 5 article reviews
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    Image Search Results


    The identity and distribution of DNA nucleotide mismatches in individual Illumina GA reads compared to the CPC98 consensus mtDNA genome. (A) The number and proportion of each nucleotide called in the Illumina GA reads (vertical column) compared to the consensus mtDNA sequence (horizontal column) is presented. (B) Mean percentage of discordant nucleotides for each position across all individual Illumina GA sequence reads.

    Journal: PLoS ONE

    Article Title: A Complete Mitochondrial Genome Sequence from a Mesolithic Wild Aurochs (Bos primigenius)

    doi: 10.1371/journal.pone.0009255

    Figure Lengend Snippet: The identity and distribution of DNA nucleotide mismatches in individual Illumina GA reads compared to the CPC98 consensus mtDNA genome. (A) The number and proportion of each nucleotide called in the Illumina GA reads (vertical column) compared to the consensus mtDNA sequence (horizontal column) is presented. (B) Mean percentage of discordant nucleotides for each position across all individual Illumina GA sequence reads.

    Article Snippet: Illumina Genome Analyzer DNA library preparations via PCR enrichment of purified end-repaired, adaptor-ligated DNA templates Individual Illumina GA libraries (n = 9) were produced via PCR enrichment of the end-repaired adaptor-ligated DNA templates prior to sequencing.

    Techniques: Sequencing

    Cloning of a miRNA sgRNA library using the Molecular Chipper method. ( a ) Overview of the Molecular Chipper method to generate a sgRNA library from pieces of input DNA. ( b ) Detailed schematics of the Molecular Chipper procedure. Briefly, an EcoP15I-site-containing adaptor is ligated to randomly fragmented DNA ends, and enzymatically released 20 bases (a G base plus 19 bases from ends of DNA fragments) are cloned as a pool into a viral vector. ( c ) Seventeen murine miRNAs (or miRNA cluster) and their flanking genomic sequences were used to generate a sgRNA library. Length distribution of the targeting portions of sgRNAs within the library is shown. Note that the length was calculated by one base G (in adaptor) plus the length of random ends of fragments from input DNA. The counts for each length are normalized to those of the 20-base-targeting motif sgRNAs within each biological replicate. Error bars represent s.d. N =3 biological replicates. ( d ) The distributions of the distances between neighbouring sgRNAs with NGG-PAM, based on all sgRNAs detected in deep sequencing, are shown (red line). The median neighbour distance is 8 bp. Theoretical distribution assumes all possible NGG-PAM sgRNAs (blue line) are present. ( e ) Top: diagram showing that the 17 murine miRNAs (or miRNA cluster) and their flanking genomic sequences were used to generate a sgRNA library. Bottom: representative graphs of sgRNA counts mapping to the miR-142 region or to the miR-126 region from one out of three neg-GFP samples is shown, with blue and red indicating mapping to sense and antisense strands, respectively. The positions of sgRNAs plotted were only based on positions of the last targeting domain base.

    Journal: Nature Communications

    Article Title: A Molecular Chipper technology for CRISPR sgRNA library generation and functional mapping of noncoding regions

    doi: 10.1038/ncomms11178

    Figure Lengend Snippet: Cloning of a miRNA sgRNA library using the Molecular Chipper method. ( a ) Overview of the Molecular Chipper method to generate a sgRNA library from pieces of input DNA. ( b ) Detailed schematics of the Molecular Chipper procedure. Briefly, an EcoP15I-site-containing adaptor is ligated to randomly fragmented DNA ends, and enzymatically released 20 bases (a G base plus 19 bases from ends of DNA fragments) are cloned as a pool into a viral vector. ( c ) Seventeen murine miRNAs (or miRNA cluster) and their flanking genomic sequences were used to generate a sgRNA library. Length distribution of the targeting portions of sgRNAs within the library is shown. Note that the length was calculated by one base G (in adaptor) plus the length of random ends of fragments from input DNA. The counts for each length are normalized to those of the 20-base-targeting motif sgRNAs within each biological replicate. Error bars represent s.d. N =3 biological replicates. ( d ) The distributions of the distances between neighbouring sgRNAs with NGG-PAM, based on all sgRNAs detected in deep sequencing, are shown (red line). The median neighbour distance is 8 bp. Theoretical distribution assumes all possible NGG-PAM sgRNAs (blue line) are present. ( e ) Top: diagram showing that the 17 murine miRNAs (or miRNA cluster) and their flanking genomic sequences were used to generate a sgRNA library. Bottom: representative graphs of sgRNA counts mapping to the miR-142 region or to the miR-126 region from one out of three neg-GFP samples is shown, with blue and red indicating mapping to sense and antisense strands, respectively. The positions of sgRNAs plotted were only based on positions of the last targeting domain base.

    Article Snippet: An amount of 5 μg of the EcoP15I-adaptor-ligated gel-purified DNA was digested by 100 units of EcoP15I enzyme (NEB) in a 300-μl reaction for 1 h at 37 °C.

    Techniques: Clone Assay, Plasmid Preparation, Genomic Sequencing, Sequencing