active center-specific monoclonal antibody Takara Search Results


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    Image Search Results


    Effect of SNAP on the levels of xCT and 4F2hc proteins in RGC-5 cells

    Journal:

    Article Title: Expression of the glutamate-cystine (xc-) exchanger in cultured retinal ganglion cells and regulation by nitric oxide and oxidative stress

    doi: 10.1007/s00441-005-0116-x

    Figure Lengend Snippet: Effect of SNAP on the levels of xCT and 4F2hc proteins in RGC-5 cells

    Article Snippet: Reagents used in this study were obtained from the following sources: Dulbecco's modified Eagle's medium F12 (DMEM:F12) medium, Earl's Balanced Salt Solution, Dulbecco's Phosphate Buffered Saline, Neurobasal medium, TRIzol reagent, penicillin-streptomycin (Gibco-Life Technologies, Rockville, MD); [2,3-3 H]-glutamate (specific radioactivity 50.0 Ci/mmol) (Amersham Bioscience, Arlington Heights, IL), L-[4,5-3H(N)]-leucine (specific radioactivity 60 Ci/mmol) (New England Nuclear, Boston, MA), L-[2,3-3H]-alanine (specific radioactivity 40 Ci/mmol) (Moravek Biochemicals, Brea, CA); 4F2hc (CD98) goat polyclonal IgG, goat anti-rabbit IgG-HRP, goat anti-mouse IgG-HRP (Santa Cruz Biotechnology, Inc, Santa Cruz, CA); Cy-3 conjugated anti-rabbbit IgG, peroxidase-conjugated AffiniPure rabbit anti-goat IgG, Alexa Fluor 488-conjugated anti-goat IgG (In Vitrogen, Carlsbad, CA); Hoeschst dye 33342 (Molecular Probes, Eugene, OR); ECL detection kit (Pierce Biotechnology, Inc., Rockford, IL); Bio-Rad Protein Assay Reagent (Bio-Rad, Hercules, CA); Power Block (BioGenex, San Ramon, CA); Glutathione (GSH) Detection Kit (Chemicon International, Temecula, CA); complete Mini Protease Inhibitor Cocktail tablets, GeneAmp RNA PCR Kit (Applied Biosystems/Roche Molecular System, Branchburg, NJ); TaKaRa Taq™ (Takara Bio Inc, Otsu, Shiga, Japan); sense and antisense primers for xCT and 4F2hc (Integrated DNA Technologies, Inc, Coralville, IA); papain (Worthington, Lakewood, NJ), trypsin inhibitor (Roche Applied Science, Mannheim, Germany), rabbit anti-macrophage antiserum (Accurate Chemical Corp., Westbury, NY), AffiniPure donkey anti-rabbit IgG (H+L) (Jackson, ImmunoResearch, Westgrove, PA), purified rat anti-mouse Thy 1.2 antibody (Pharmingen, San Diego, CA), brain-derived neurotrophic factor, ciliary-derived neurotrophic factor (basic fibroblast growth factor (PeproTech Inc., Rocky Hill, NJ), 3-nitroso-N-acetylpenicillamine (SNAP), S-nitrosoglutathione (SNOG), 3-Morpholinosydnonimine hydrochloride (SIN-1), menadione sodium bisulfite, hydrogen peroxide 30% (W/W) solution, xanthine sodium salt, xanthine oxidase, fetal bovine serum, monoclonal antibody to β-actin and all other chemicals (Sigma-Aldrich Chemical Co., St. Louis, MO); RGC-5 cells were the kind gift of Dr. Neeraj Agarwal, Department of Anatomy and Cell Biology, University of North Texas Health Science Center, Fort Worth, TX.

    Techniques: