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  • 94
    Waters Corporation acquity uplc
    <t>RP-UPLC-DAD</t> profiles of hydroethanolic extract of B. trimera . Conditions: CHS130 100 RP-18 column (1.7 μ m, 50 × 3 mm i.d.). Elution was carried out with a linear gradient of water 0.1% formic acid (A) and acetonitrile 0.1% formic acid (B) (from 5% to 95% of B in 11 min) and the UPLC fingerprints were registered on a <t>ACQUITY</t> Waters apparatus with a UV-DAD detector (Waters 2996). Operating parameters of the mass spectrometer were capillary temperature 320°C; spray needle voltage set at 3.50 kV; ES capillary voltage +3 and −47 V for positive and negative polarity, respectively; and tube lens offset 0 and −25 V for positive and negative polarity, respectively. Nitrogen was used as a sheath gas with a flow of 50 arbitrary units. Mass analysis was carried out in full-scan mode from 100 to 1.500 amu, in both positive and negative mode. UV spectra (190–450 nm) from the main peaks are shown inside on the chromatogram.
    Acquity Uplc, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 3267 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Waters Corporation acquity hclass uplc
    <t>RP-UPLC-DAD</t> profiles of hydroethanolic extract of B. trimera . Conditions: CHS130 100 RP-18 column (1.7 μ m, 50 × 3 mm i.d.). Elution was carried out with a linear gradient of water 0.1% formic acid (A) and acetonitrile 0.1% formic acid (B) (from 5% to 95% of B in 11 min) and the UPLC fingerprints were registered on a <t>ACQUITY</t> Waters apparatus with a UV-DAD detector (Waters 2996). Operating parameters of the mass spectrometer were capillary temperature 320°C; spray needle voltage set at 3.50 kV; ES capillary voltage +3 and −47 V for positive and negative polarity, respectively; and tube lens offset 0 and −25 V for positive and negative polarity, respectively. Nitrogen was used as a sheath gas with a flow of 50 arbitrary units. Mass analysis was carried out in full-scan mode from 100 to 1.500 amu, in both positive and negative mode. UV spectra (190–450 nm) from the main peaks are shown inside on the chromatogram.
    Acquity Hclass Uplc, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Waters Corporation acquity uplc behc18
    <t>RP-UPLC-DAD</t> profiles of hydroethanolic extract of B. trimera . Conditions: CHS130 100 RP-18 column (1.7 μ m, 50 × 3 mm i.d.). Elution was carried out with a linear gradient of water 0.1% formic acid (A) and acetonitrile 0.1% formic acid (B) (from 5% to 95% of B in 11 min) and the UPLC fingerprints were registered on a <t>ACQUITY</t> Waters apparatus with a UV-DAD detector (Waters 2996). Operating parameters of the mass spectrometer were capillary temperature 320°C; spray needle voltage set at 3.50 kV; ES capillary voltage +3 and −47 V for positive and negative polarity, respectively; and tube lens offset 0 and −25 V for positive and negative polarity, respectively. Nitrogen was used as a sheath gas with a flow of 50 arbitrary units. Mass analysis was carried out in full-scan mode from 100 to 1.500 amu, in both positive and negative mode. UV spectra (190–450 nm) from the main peaks are shown inside on the chromatogram.
    Acquity Uplc Behc18, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 92/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Waters Corporation acquity uplc column
    <t>RP-UPLC-DAD</t> profiles of hydroethanolic extract of B. trimera . Conditions: CHS130 100 RP-18 column (1.7 μ m, 50 × 3 mm i.d.). Elution was carried out with a linear gradient of water 0.1% formic acid (A) and acetonitrile 0.1% formic acid (B) (from 5% to 95% of B in 11 min) and the UPLC fingerprints were registered on a <t>ACQUITY</t> Waters apparatus with a UV-DAD detector (Waters 2996). Operating parameters of the mass spectrometer were capillary temperature 320°C; spray needle voltage set at 3.50 kV; ES capillary voltage +3 and −47 V for positive and negative polarity, respectively; and tube lens offset 0 and −25 V for positive and negative polarity, respectively. Nitrogen was used as a sheath gas with a flow of 50 arbitrary units. Mass analysis was carried out in full-scan mode from 100 to 1.500 amu, in both positive and negative mode. UV spectra (190–450 nm) from the main peaks are shown inside on the chromatogram.
    Acquity Uplc Column, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 88/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation nano acquity uplc
    <t>RP-UPLC-DAD</t> profiles of hydroethanolic extract of B. trimera . Conditions: CHS130 100 RP-18 column (1.7 μ m, 50 × 3 mm i.d.). Elution was carried out with a linear gradient of water 0.1% formic acid (A) and acetonitrile 0.1% formic acid (B) (from 5% to 95% of B in 11 min) and the UPLC fingerprints were registered on a <t>ACQUITY</t> Waters apparatus with a UV-DAD detector (Waters 2996). Operating parameters of the mass spectrometer were capillary temperature 320°C; spray needle voltage set at 3.50 kV; ES capillary voltage +3 and −47 V for positive and negative polarity, respectively; and tube lens offset 0 and −25 V for positive and negative polarity, respectively. Nitrogen was used as a sheath gas with a flow of 50 arbitrary units. Mass analysis was carried out in full-scan mode from 100 to 1.500 amu, in both positive and negative mode. UV spectra (190–450 nm) from the main peaks are shown inside on the chromatogram.
    Nano Acquity Uplc, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 90/100, based on 483 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation acquity uplc beh
    <t>RP-UPLC-DAD</t> profiles of hydroethanolic extract of B. trimera . Conditions: CHS130 100 RP-18 column (1.7 μ m, 50 × 3 mm i.d.). Elution was carried out with a linear gradient of water 0.1% formic acid (A) and acetonitrile 0.1% formic acid (B) (from 5% to 95% of B in 11 min) and the UPLC fingerprints were registered on a <t>ACQUITY</t> Waters apparatus with a UV-DAD detector (Waters 2996). Operating parameters of the mass spectrometer were capillary temperature 320°C; spray needle voltage set at 3.50 kV; ES capillary voltage +3 and −47 V for positive and negative polarity, respectively; and tube lens offset 0 and −25 V for positive and negative polarity, respectively. Nitrogen was used as a sheath gas with a flow of 50 arbitrary units. Mass analysis was carried out in full-scan mode from 100 to 1.500 amu, in both positive and negative mode. UV spectra (190–450 nm) from the main peaks are shown inside on the chromatogram.
    Acquity Uplc Beh, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 90/100, based on 207 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation acquity uplc tqd
    <t>RP-UPLC-DAD</t> profiles of hydroethanolic extract of B. trimera . Conditions: CHS130 100 RP-18 column (1.7 μ m, 50 × 3 mm i.d.). Elution was carried out with a linear gradient of water 0.1% formic acid (A) and acetonitrile 0.1% formic acid (B) (from 5% to 95% of B in 11 min) and the UPLC fingerprints were registered on a <t>ACQUITY</t> Waters apparatus with a UV-DAD detector (Waters 2996). Operating parameters of the mass spectrometer were capillary temperature 320°C; spray needle voltage set at 3.50 kV; ES capillary voltage +3 and −47 V for positive and negative polarity, respectively; and tube lens offset 0 and −25 V for positive and negative polarity, respectively. Nitrogen was used as a sheath gas with a flow of 50 arbitrary units. Mass analysis was carried out in full-scan mode from 100 to 1.500 amu, in both positive and negative mode. UV spectra (190–450 nm) from the main peaks are shown inside on the chromatogram.
    Acquity Uplc Tqd, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Agilent technologies waters acquity uplc
    <t>RP-UPLC-DAD</t> profiles of hydroethanolic extract of B. trimera . Conditions: CHS130 100 RP-18 column (1.7 μ m, 50 × 3 mm i.d.). Elution was carried out with a linear gradient of water 0.1% formic acid (A) and acetonitrile 0.1% formic acid (B) (from 5% to 95% of B in 11 min) and the UPLC fingerprints were registered on a <t>ACQUITY</t> Waters apparatus with a UV-DAD detector (Waters 2996). Operating parameters of the mass spectrometer were capillary temperature 320°C; spray needle voltage set at 3.50 kV; ES capillary voltage +3 and −47 V for positive and negative polarity, respectively; and tube lens offset 0 and −25 V for positive and negative polarity, respectively. Nitrogen was used as a sheath gas with a flow of 50 arbitrary units. Mass analysis was carried out in full-scan mode from 100 to 1.500 amu, in both positive and negative mode. UV spectra (190–450 nm) from the main peaks are shown inside on the chromatogram.
    Waters Acquity Uplc, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation acquity uplc chromatograph
    <t>RP-UPLC-DAD</t> profiles of hydroethanolic extract of B. trimera . Conditions: CHS130 100 RP-18 column (1.7 μ m, 50 × 3 mm i.d.). Elution was carried out with a linear gradient of water 0.1% formic acid (A) and acetonitrile 0.1% formic acid (B) (from 5% to 95% of B in 11 min) and the UPLC fingerprints were registered on a <t>ACQUITY</t> Waters apparatus with a UV-DAD detector (Waters 2996). Operating parameters of the mass spectrometer were capillary temperature 320°C; spray needle voltage set at 3.50 kV; ES capillary voltage +3 and −47 V for positive and negative polarity, respectively; and tube lens offset 0 and −25 V for positive and negative polarity, respectively. Nitrogen was used as a sheath gas with a flow of 50 arbitrary units. Mass analysis was carried out in full-scan mode from 100 to 1.500 amu, in both positive and negative mode. UV spectra (190–450 nm) from the main peaks are shown inside on the chromatogram.
    Acquity Uplc Chromatograph, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 85/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Waters Corporation acquity uplc hss
    <t>RP-UPLC-DAD</t> profiles of hydroethanolic extract of B. trimera . Conditions: CHS130 100 RP-18 column (1.7 μ m, 50 × 3 mm i.d.). Elution was carried out with a linear gradient of water 0.1% formic acid (A) and acetonitrile 0.1% formic acid (B) (from 5% to 95% of B in 11 min) and the UPLC fingerprints were registered on a <t>ACQUITY</t> Waters apparatus with a UV-DAD detector (Waters 2996). Operating parameters of the mass spectrometer were capillary temperature 320°C; spray needle voltage set at 3.50 kV; ES capillary voltage +3 and −47 V for positive and negative polarity, respectively; and tube lens offset 0 and −25 V for positive and negative polarity, respectively. Nitrogen was used as a sheath gas with a flow of 50 arbitrary units. Mass analysis was carried out in full-scan mode from 100 to 1.500 amu, in both positive and negative mode. UV spectra (190–450 nm) from the main peaks are shown inside on the chromatogram.
    Acquity Uplc Hss, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation acquity uplc machine
    <t>RP-UPLC-DAD</t> profiles of hydroethanolic extract of B. trimera . Conditions: CHS130 100 RP-18 column (1.7 μ m, 50 × 3 mm i.d.). Elution was carried out with a linear gradient of water 0.1% formic acid (A) and acetonitrile 0.1% formic acid (B) (from 5% to 95% of B in 11 min) and the UPLC fingerprints were registered on a <t>ACQUITY</t> Waters apparatus with a UV-DAD detector (Waters 2996). Operating parameters of the mass spectrometer were capillary temperature 320°C; spray needle voltage set at 3.50 kV; ES capillary voltage +3 and −47 V for positive and negative polarity, respectively; and tube lens offset 0 and −25 V for positive and negative polarity, respectively. Nitrogen was used as a sheath gas with a flow of 50 arbitrary units. Mass analysis was carried out in full-scan mode from 100 to 1.500 amu, in both positive and negative mode. UV spectra (190–450 nm) from the main peaks are shown inside on the chromatogram.
    Acquity Uplc Machine, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 90/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation uplc acquity chromatographer
    <t>RP-UPLC-DAD</t> profiles of hydroethanolic extract of B. trimera . Conditions: CHS130 100 RP-18 column (1.7 μ m, 50 × 3 mm i.d.). Elution was carried out with a linear gradient of water 0.1% formic acid (A) and acetonitrile 0.1% formic acid (B) (from 5% to 95% of B in 11 min) and the UPLC fingerprints were registered on a <t>ACQUITY</t> Waters apparatus with a UV-DAD detector (Waters 2996). Operating parameters of the mass spectrometer were capillary temperature 320°C; spray needle voltage set at 3.50 kV; ES capillary voltage +3 and −47 V for positive and negative polarity, respectively; and tube lens offset 0 and −25 V for positive and negative polarity, respectively. Nitrogen was used as a sheath gas with a flow of 50 arbitrary units. Mass analysis was carried out in full-scan mode from 100 to 1.500 amu, in both positive and negative mode. UV spectra (190–450 nm) from the main peaks are shown inside on the chromatogram.
    Uplc Acquity Chromatographer, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    RP-UPLC-DAD profiles of hydroethanolic extract of B. trimera . Conditions: CHS130 100 RP-18 column (1.7 μ m, 50 × 3 mm i.d.). Elution was carried out with a linear gradient of water 0.1% formic acid (A) and acetonitrile 0.1% formic acid (B) (from 5% to 95% of B in 11 min) and the UPLC fingerprints were registered on a ACQUITY Waters apparatus with a UV-DAD detector (Waters 2996). Operating parameters of the mass spectrometer were capillary temperature 320°C; spray needle voltage set at 3.50 kV; ES capillary voltage +3 and −47 V for positive and negative polarity, respectively; and tube lens offset 0 and −25 V for positive and negative polarity, respectively. Nitrogen was used as a sheath gas with a flow of 50 arbitrary units. Mass analysis was carried out in full-scan mode from 100 to 1.500 amu, in both positive and negative mode. UV spectra (190–450 nm) from the main peaks are shown inside on the chromatogram.

    Journal: Mediators of Inflammation

    Article Title: Protective Effect of Baccharis trimera Extract on Acute Hepatic Injury in a Model of Inflammation Induced by Acetaminophen

    doi: 10.1155/2014/196598

    Figure Lengend Snippet: RP-UPLC-DAD profiles of hydroethanolic extract of B. trimera . Conditions: CHS130 100 RP-18 column (1.7 μ m, 50 × 3 mm i.d.). Elution was carried out with a linear gradient of water 0.1% formic acid (A) and acetonitrile 0.1% formic acid (B) (from 5% to 95% of B in 11 min) and the UPLC fingerprints were registered on a ACQUITY Waters apparatus with a UV-DAD detector (Waters 2996). Operating parameters of the mass spectrometer were capillary temperature 320°C; spray needle voltage set at 3.50 kV; ES capillary voltage +3 and −47 V for positive and negative polarity, respectively; and tube lens offset 0 and −25 V for positive and negative polarity, respectively. Nitrogen was used as a sheath gas with a flow of 50 arbitrary units. Mass analysis was carried out in full-scan mode from 100 to 1.500 amu, in both positive and negative mode. UV spectra (190–450 nm) from the main peaks are shown inside on the chromatogram.

    Article Snippet: The ESI-MS/MS analyses were additionally performed in an UPLC Acquity (Waters) with helium as the collision gas, and the collision energy was set at 30 eV.

    Techniques: Mass Spectrometry, Flow Cytometry

    Total and free amino acid content of transgenic and wild-type cassava roots. (A) Measurement of total protein hydrolyzed and free amino acid concentrations in transgenic and wild-type roots. Samples were hydrolyzed with HCl and subjected to ACQUITY UPLC® System. Data are presented as nmoles/mg total dry weight. Error bars indicate SE of the mean of two biological replicates. The asterisk (*) indicates statistically significant differences between wild-type and transgenics, determined by Student's t-test, with P

    Journal: PLoS ONE

    Article Title: Overexpression of Hydroxynitrile Lyase in Cassava Roots Elevates Protein and Free Amino Acids while Reducing Residual Cyanogen Levels

    doi: 10.1371/journal.pone.0021996

    Figure Lengend Snippet: Total and free amino acid content of transgenic and wild-type cassava roots. (A) Measurement of total protein hydrolyzed and free amino acid concentrations in transgenic and wild-type roots. Samples were hydrolyzed with HCl and subjected to ACQUITY UPLC® System. Data are presented as nmoles/mg total dry weight. Error bars indicate SE of the mean of two biological replicates. The asterisk (*) indicates statistically significant differences between wild-type and transgenics, determined by Student's t-test, with P

    Article Snippet: Samples were dried and resuspended in 20 mM HCl, derivatized with the AccQ-tag reagent (Waters) and separated by ACQUITY UPLC® System (Waters, Milford, MA, USA) according to manufacturer's instructions.

    Techniques: Transgenic Assay

    Example of a chromatographic separation of atracurium besylate stereoisomers using an Agilent Technologies Zorbax Eclipse XDB-C18 50 mm×4.6 mm column with 1.8 μm particle, using a potassium phosphate buffer–acetonitrile–methanol gradient at a flow rate of 1.0 mL/min and UV detection at 280 nm. Example of a chromatographic separation of a degraded solution of cis–cis atracurium besylate exposed to a temperature of 80 °C for 60 min. The same chromatographic conditions as those used in (a) were used. The peak eluting at 1.6 min corresponds to laudanosine (RRT=0.27); the peak eluting at 7.4 min corresponds to monoacrylate laudanosine besylate (RRT 1.26). Separation of a peptide from phenol preservative in a multi-dose formulation. Experimental conditions: Waters Acquity UPLC ® BEH C18 column (50 mm×2.1 mm, with 1.7 μm hybrid silica particles), using a mobile phase gradient with 0.1% aqueous phosphoric acid and acetonitrile in 4 min, at a flow rate of 0.6 mL/min, with an injection volume of 1 μL and detection wavelength at 220 nm. Solution concentration: 50 μg/mL.

    Journal: Journal of Pharmaceutical Analysis

    Article Title: Impurity profiling and in-process testing of drugs for injection by fast liquid chromatography

    doi: 10.1016/j.jpha.2012.07.004

    Figure Lengend Snippet: Example of a chromatographic separation of atracurium besylate stereoisomers using an Agilent Technologies Zorbax Eclipse XDB-C18 50 mm×4.6 mm column with 1.8 μm particle, using a potassium phosphate buffer–acetonitrile–methanol gradient at a flow rate of 1.0 mL/min and UV detection at 280 nm. Example of a chromatographic separation of a degraded solution of cis–cis atracurium besylate exposed to a temperature of 80 °C for 60 min. The same chromatographic conditions as those used in (a) were used. The peak eluting at 1.6 min corresponds to laudanosine (RRT=0.27); the peak eluting at 7.4 min corresponds to monoacrylate laudanosine besylate (RRT 1.26). Separation of a peptide from phenol preservative in a multi-dose formulation. Experimental conditions: Waters Acquity UPLC ® BEH C18 column (50 mm×2.1 mm, with 1.7 μm hybrid silica particles), using a mobile phase gradient with 0.1% aqueous phosphoric acid and acetonitrile in 4 min, at a flow rate of 0.6 mL/min, with an injection volume of 1 μL and detection wavelength at 220 nm. Solution concentration: 50 μg/mL.

    Article Snippet: 2 Materials and method The experimental results reported in this paper were obtained on Waters Acquity UPLC® (Milford, MA, USA) systems with either a Photodiode Array Detector or tunable UV (TUV) detector.

    Techniques: Flow Cytometry, Injection, Concentration Assay

    Example of chromatographic separation of a taxane from known impurities and degradants on a Waters Acquity UPLC ® BEH C18 column (100 mm×2.1 mm), using a water/acetonitrile gradient at a flow rate of 0.6 mL/min and UV detection at 228 nm.

    Journal: Journal of Pharmaceutical Analysis

    Article Title: Impurity profiling and in-process testing of drugs for injection by fast liquid chromatography

    doi: 10.1016/j.jpha.2012.07.004

    Figure Lengend Snippet: Example of chromatographic separation of a taxane from known impurities and degradants on a Waters Acquity UPLC ® BEH C18 column (100 mm×2.1 mm), using a water/acetonitrile gradient at a flow rate of 0.6 mL/min and UV detection at 228 nm.

    Article Snippet: 2 Materials and method The experimental results reported in this paper were obtained on Waters Acquity UPLC® (Milford, MA, USA) systems with either a Photodiode Array Detector or tunable UV (TUV) detector.

    Techniques: Flow Cytometry

    RP-UPLC-DAD profiles of hydroethanolic extract of B. trimera . Conditions: CHS130 100 RP-18 column (1.7 μ m, 50 × 3 mm i.d.). Elution was carried out with a linear gradient of water 0.1% formic acid (A) and acetonitrile 0.1% formic acid (B) (from 5% to 95% of B in 11 min) and the UPLC fingerprints were registered on a ACQUITY Waters apparatus with a UV-DAD detector (Waters 2996). Operating parameters of the mass spectrometer were capillary temperature 320°C; spray needle voltage set at 3.50 kV; ES capillary voltage +3 and −47 V for positive and negative polarity, respectively; and tube lens offset 0 and −25 V for positive and negative polarity, respectively. Nitrogen was used as a sheath gas with a flow of 50 arbitrary units. Mass analysis was carried out in full-scan mode from 100 to 1.500 amu, in both positive and negative mode. UV spectra (190–450 nm) from the main peaks are shown inside on the chromatogram.

    Journal: Mediators of Inflammation

    Article Title: Protective Effect of Baccharis trimera Extract on Acute Hepatic Injury in a Model of Inflammation Induced by Acetaminophen

    doi: 10.1155/2014/196598

    Figure Lengend Snippet: RP-UPLC-DAD profiles of hydroethanolic extract of B. trimera . Conditions: CHS130 100 RP-18 column (1.7 μ m, 50 × 3 mm i.d.). Elution was carried out with a linear gradient of water 0.1% formic acid (A) and acetonitrile 0.1% formic acid (B) (from 5% to 95% of B in 11 min) and the UPLC fingerprints were registered on a ACQUITY Waters apparatus with a UV-DAD detector (Waters 2996). Operating parameters of the mass spectrometer were capillary temperature 320°C; spray needle voltage set at 3.50 kV; ES capillary voltage +3 and −47 V for positive and negative polarity, respectively; and tube lens offset 0 and −25 V for positive and negative polarity, respectively. Nitrogen was used as a sheath gas with a flow of 50 arbitrary units. Mass analysis was carried out in full-scan mode from 100 to 1.500 amu, in both positive and negative mode. UV spectra (190–450 nm) from the main peaks are shown inside on the chromatogram.

    Article Snippet: Chromatographic separation was done on ACQUITY UPLC BEH (1.7 μ m, 50 × 2 mm i.d.) (Waters).

    Techniques: Mass Spectrometry, Flow Cytometry