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  • 99
    Thermo Fisher acn
    Acn, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 463 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher tbapf6 acn
    Tbapf6 Acn, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    scharlau acetronile acn
    Acetronile Acn, supplied by scharlau, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Merck KGaA acetonitril acn
    Acetonitril Acn, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Avantor acetonitrile acn
    Separation of the enantiomers of RS/SR phenylpropanolamine (Solute #26 in Table 1 ) on a 25 cm Larihc CF6-P column in PO mode with (A) <t>ACN/</t> <t>MeOH/TFA/TEA</t> (90:10:0.3:0.2, v/v/v/v) and (B) ACN/MeOH/AA/TEA (60:40:0.3:0.2, v/v/v/v). Flow rate: 2 mL/min; detection UV: 254 nm.
    Acetonitrile Acn, supplied by Avantor, used in various techniques. Bioz Stars score: 93/100, based on 319 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Biosolve acetonitrile acn
    Separation of the enantiomers of RS/SR phenylpropanolamine (Solute #26 in Table 1 ) on a 25 cm Larihc CF6-P column in PO mode with (A) <t>ACN/</t> <t>MeOH/TFA/TEA</t> (90:10:0.3:0.2, v/v/v/v) and (B) ACN/MeOH/AA/TEA (60:40:0.3:0.2, v/v/v/v). Flow rate: 2 mL/min; detection UV: 254 nm.
    Acetonitrile Acn, supplied by Biosolve, used in various techniques. Bioz Stars score: 93/100, based on 309 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Fisher Scientific acetonitrile acn
    Chromatographic separation of (A) 18 SPMs (100 nM each) and (B) 5 deuterated internal standards (20 nM) covered by the method including (C) DHA derived D-series resolvins, (D) EPA derived E-series resolvins, (E) EPA and ARA derived lipoxins, DHA derived (F) protectins, and (G) maresins. Separation was carried out on an RP-18 column (2.1 × 150 mm, particle size 1.8 μm, pore size 9.5 nm) with a H 2 <t>O/MeOH/ACN/HAc</t> gradient.
    Acetonitrile Acn, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 719 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Merck KGaA acetonitrile acn
    <t>HPLC</t> profiles of the fragmentation of LK by selected Saps. LK samples (1.5 μM) were digested with 0.03 μM of Sap1, −3, −4, −8, and −9 in the citrate buffer (pH 5.0) at 37°C for 24 hours. The reaction was stopped using pepstatin A (10 μM), followed by acidification with HCl (0.33 M). The samples were analyzed using HPLC on the Luna C18(2) 5 μm 4.6 × 250 mm column (Phenomenex) in a TFA <t>water-ACN</t> binary gradient system, as described in the Materials and Methods section. Arrows indicate the retention times of the Met-Lys-bradykinin (M) and des-Arg 1 -bradykinin (D) standards.
    Acetonitrile Acn, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 533 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Tedia acetonitrile acn
    RP-HPLC analysis of PEGylated HM-3. Analysis was performed on a C18 column with <t>water/ACN/TFA</t> as eluent and a flow rate of 1 mL/min, and peaks were monitored at 220 nm. ( A ) ALD-mPEG 5k -HM-3 purity: 97.39%, T: 9.395 min; ( B ) ALD-mPEG 10k -HM-3 purity: 98.23%, T: 9.698 min; ( C ) SC-mPEG 10k -HM-3 purity: 97.02%, T: 9.883 min; ( D ) SC-mPEG 20k -HM-3 purity: 98.52%, T: 9.578 min.
    Acetonitrile Acn, supplied by Tedia, used in various techniques. Bioz Stars score: 92/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Burdick & Jackson acetonitrile acn
    <t>HPLC</t> chromatograms: ( a ) condition 1 with Agilent XDB-C18 at an isocratic mobile phase of acetonitrile <t>(ACN):water</t> (0.7% trifluoroacetic acid (TFA), 5 mM heptafluorobutyric acid (HFBA)) = 2:98; ( b ) condition 2 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 2:98; ( c ) condition 3 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 9:91; ( d ) after approximately 48 hours of repeated measurements with condition 3.
    Acetonitrile Acn, supplied by Burdick & Jackson, used in various techniques. Bioz Stars score: 93/100, based on 188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    RATHBURN CHEMICALS LIMITED acetonitrile acn
    <t>HPLC</t> chromatograms: ( a ) condition 1 with Agilent XDB-C18 at an isocratic mobile phase of acetonitrile <t>(ACN):water</t> (0.7% trifluoroacetic acid (TFA), 5 mM heptafluorobutyric acid (HFBA)) = 2:98; ( b ) condition 2 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 2:98; ( c ) condition 3 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 9:91; ( d ) after approximately 48 hours of repeated measurements with condition 3.
    Acetonitrile Acn, supplied by RATHBURN CHEMICALS LIMITED, used in various techniques. Bioz Stars score: 92/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Sinopharm acetonitrile acn
    <t>HPLC</t> chromatograms: ( a ) condition 1 with Agilent XDB-C18 at an isocratic mobile phase of acetonitrile <t>(ACN):water</t> (0.7% trifluoroacetic acid (TFA), 5 mM heptafluorobutyric acid (HFBA)) = 2:98; ( b ) condition 2 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 2:98; ( c ) condition 3 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 9:91; ( d ) after approximately 48 hours of repeated measurements with condition 3.
    Acetonitrile Acn, supplied by Sinopharm, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Agilent technologies acetonitrile acn
    <t>HPLC</t> chromatograms: ( a ) condition 1 with Agilent XDB-C18 at an isocratic mobile phase of acetonitrile <t>(ACN):water</t> (0.7% trifluoroacetic acid (TFA), 5 mM heptafluorobutyric acid (HFBA)) = 2:98; ( b ) condition 2 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 2:98; ( c ) condition 3 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 9:91; ( d ) after approximately 48 hours of repeated measurements with condition 3.
    Acetonitrile Acn, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Carlo Erba Reagents acetonitrile acn
    <t>HPLC</t> chromatograms: ( a ) condition 1 with Agilent XDB-C18 at an isocratic mobile phase of acetonitrile <t>(ACN):water</t> (0.7% trifluoroacetic acid (TFA), 5 mM heptafluorobutyric acid (HFBA)) = 2:98; ( b ) condition 2 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 2:98; ( c ) condition 3 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 9:91; ( d ) after approximately 48 hours of repeated measurements with condition 3.
    Acetonitrile Acn, supplied by Carlo Erba Reagents, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Duksan Pure Chemicals acetonitrile acn
    <t>HPLC</t> chromatograms: ( a ) condition 1 with Agilent XDB-C18 at an isocratic mobile phase of acetonitrile <t>(ACN):water</t> (0.7% trifluoroacetic acid (TFA), 5 mM heptafluorobutyric acid (HFBA)) = 2:98; ( b ) condition 2 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 2:98; ( c ) condition 3 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 9:91; ( d ) after approximately 48 hours of repeated measurements with condition 3.
    Acetonitrile Acn, supplied by Duksan Pure Chemicals, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    GFS Chemicals acetonitrile acn
    <t>HPLC</t> chromatograms: ( a ) condition 1 with Agilent XDB-C18 at an isocratic mobile phase of acetonitrile <t>(ACN):water</t> (0.7% trifluoroacetic acid (TFA), 5 mM heptafluorobutyric acid (HFBA)) = 2:98; ( b ) condition 2 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 2:98; ( c ) condition 3 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 9:91; ( d ) after approximately 48 hours of repeated measurements with condition 3.
    Acetonitrile Acn, supplied by GFS Chemicals, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Fisher Scientific acn v v
    <t>HPLC</t> chromatograms: ( a ) condition 1 with Agilent XDB-C18 at an isocratic mobile phase of acetonitrile <t>(ACN):water</t> (0.7% trifluoroacetic acid (TFA), 5 mM heptafluorobutyric acid (HFBA)) = 2:98; ( b ) condition 2 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 2:98; ( c ) condition 3 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 9:91; ( d ) after approximately 48 hours of repeated measurements with condition 3.
    Acn V V, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck & Co acetonitrile acn
    <t>HPLC</t> chromatograms: ( a ) condition 1 with Agilent XDB-C18 at an isocratic mobile phase of acetonitrile <t>(ACN):water</t> (0.7% trifluoroacetic acid (TFA), 5 mM heptafluorobutyric acid (HFBA)) = 2:98; ( b ) condition 2 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 2:98; ( c ) condition 3 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 9:91; ( d ) after approximately 48 hours of repeated measurements with condition 3.
    Acetonitrile Acn, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    PHARMCO-AAPER acetonitrile acn
    <t>HPLC</t> chromatograms: ( a ) condition 1 with Agilent XDB-C18 at an isocratic mobile phase of acetonitrile <t>(ACN):water</t> (0.7% trifluoroacetic acid (TFA), 5 mM heptafluorobutyric acid (HFBA)) = 2:98; ( b ) condition 2 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 2:98; ( c ) condition 3 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 9:91; ( d ) after approximately 48 hours of repeated measurements with condition 3.
    Acetonitrile Acn, supplied by PHARMCO-AAPER, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Fisher Scientific acrylonitrile acn
    <t>HPLC</t> chromatograms: ( a ) condition 1 with Agilent XDB-C18 at an isocratic mobile phase of acetonitrile <t>(ACN):water</t> (0.7% trifluoroacetic acid (TFA), 5 mM heptafluorobutyric acid (HFBA)) = 2:98; ( b ) condition 2 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 2:98; ( c ) condition 3 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 9:91; ( d ) after approximately 48 hours of repeated measurements with condition 3.
    Acrylonitrile Acn, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Kanto Chemical acetonitrile acn
    <t>HPLC</t> chromatograms: ( a ) condition 1 with Agilent XDB-C18 at an isocratic mobile phase of acetonitrile <t>(ACN):water</t> (0.7% trifluoroacetic acid (TFA), 5 mM heptafluorobutyric acid (HFBA)) = 2:98; ( b ) condition 2 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 2:98; ( c ) condition 3 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 9:91; ( d ) after approximately 48 hours of repeated measurements with condition 3.
    Acetonitrile Acn, supplied by Kanto Chemical, used in various techniques. Bioz Stars score: 93/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    honeywell international acetonitrile acn
    <t>HPLC</t> chromatograms: ( a ) condition 1 with Agilent XDB-C18 at an isocratic mobile phase of acetonitrile <t>(ACN):water</t> (0.7% trifluoroacetic acid (TFA), 5 mM heptafluorobutyric acid (HFBA)) = 2:98; ( b ) condition 2 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 2:98; ( c ) condition 3 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 9:91; ( d ) after approximately 48 hours of repeated measurements with condition 3.
    Acetonitrile Acn, supplied by honeywell international, used in various techniques. Bioz Stars score: 92/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Shanghai Macklin Biochemical acetonitrile acn
    <t>HPLC</t> chromatograms: ( a ) condition 1 with Agilent XDB-C18 at an isocratic mobile phase of acetonitrile <t>(ACN):water</t> (0.7% trifluoroacetic acid (TFA), 5 mM heptafluorobutyric acid (HFBA)) = 2:98; ( b ) condition 2 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 2:98; ( c ) condition 3 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 9:91; ( d ) after approximately 48 hours of repeated measurements with condition 3.
    Acetonitrile Acn, supplied by Shanghai Macklin Biochemical, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Shanghai Aladdin Bio-Chem acetonitrile acn
    <t>HPLC</t> chromatograms: ( a ) condition 1 with Agilent XDB-C18 at an isocratic mobile phase of acetonitrile <t>(ACN):water</t> (0.7% trifluoroacetic acid (TFA), 5 mM heptafluorobutyric acid (HFBA)) = 2:98; ( b ) condition 2 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 2:98; ( c ) condition 3 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 9:91; ( d ) after approximately 48 hours of repeated measurements with condition 3.
    Acetonitrile Acn, supplied by Shanghai Aladdin Bio-Chem, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Separation of the enantiomers of RS/SR phenylpropanolamine (Solute #26 in Table 1 ) on a 25 cm Larihc CF6-P column in PO mode with (A) ACN/ MeOH/TFA/TEA (90:10:0.3:0.2, v/v/v/v) and (B) ACN/MeOH/AA/TEA (60:40:0.3:0.2, v/v/v/v). Flow rate: 2 mL/min; detection UV: 254 nm.

    Journal: Journal of Pharmaceutical Analysis

    Article Title: Screening primary racemic amines for enantioseparation by derivatized polysaccharide and cyclofructan columns

    doi: 10.1016/j.jpha.2016.07.003

    Figure Lengend Snippet: Separation of the enantiomers of RS/SR phenylpropanolamine (Solute #26 in Table 1 ) on a 25 cm Larihc CF6-P column in PO mode with (A) ACN/ MeOH/TFA/TEA (90:10:0.3:0.2, v/v/v/v) and (B) ACN/MeOH/AA/TEA (60:40:0.3:0.2, v/v/v/v). Flow rate: 2 mL/min; detection UV: 254 nm.

    Article Snippet: Acetonitrile (ACN), methanol (MeOH), ethanol (EtOH), n-heptane (hept), and isopropyl alcohol (IPA) of HPLC grade were obtained from VWR (Sugarland, Texas), and used as received.

    Techniques: Flow Cytometry

    Separation of the enantiomers of (±) trans-1-Amino-2-indanol (Solute #21 in Table 1 ) on a 25 cm column in PO mode as (A) ChiralPak IB column with ACN/IPA (97:3, v/v) mobile phase with 0.1% BA and (B) Larihc CF6-P column with ACN/MeOH (90:10, v/v) mobile phase with 0.3% TFA and 0.2% TEA ionic additives. Flow rate: 2 mL/min; detection UV: 254 nm.

    Journal: Journal of Pharmaceutical Analysis

    Article Title: Screening primary racemic amines for enantioseparation by derivatized polysaccharide and cyclofructan columns

    doi: 10.1016/j.jpha.2016.07.003

    Figure Lengend Snippet: Separation of the enantiomers of (±) trans-1-Amino-2-indanol (Solute #21 in Table 1 ) on a 25 cm column in PO mode as (A) ChiralPak IB column with ACN/IPA (97:3, v/v) mobile phase with 0.1% BA and (B) Larihc CF6-P column with ACN/MeOH (90:10, v/v) mobile phase with 0.3% TFA and 0.2% TEA ionic additives. Flow rate: 2 mL/min; detection UV: 254 nm.

    Article Snippet: Acetonitrile (ACN), methanol (MeOH), ethanol (EtOH), n-heptane (hept), and isopropyl alcohol (IPA) of HPLC grade were obtained from VWR (Sugarland, Texas), and used as received.

    Techniques: Indirect Immunoperoxidase Assay, Flow Cytometry

    Separation of the RS and SR enantiomers of phenylpropanolamine (Solute #26) on a 25 cm column in PO mode as (A) ChiralPak IE column with a ACN/IPA (97:3, v/v) mobile phase with 0.1% BA and (B) Larihc CF6-P column with a ACN/MeOH (90:10, v/v) mobile phase with 0.3%TFA and 0.2%TEA additives. Flow rate: 2 mL/min; detection UV: 254 nm.

    Journal: Journal of Pharmaceutical Analysis

    Article Title: Screening primary racemic amines for enantioseparation by derivatized polysaccharide and cyclofructan columns

    doi: 10.1016/j.jpha.2016.07.003

    Figure Lengend Snippet: Separation of the RS and SR enantiomers of phenylpropanolamine (Solute #26) on a 25 cm column in PO mode as (A) ChiralPak IE column with a ACN/IPA (97:3, v/v) mobile phase with 0.1% BA and (B) Larihc CF6-P column with a ACN/MeOH (90:10, v/v) mobile phase with 0.3%TFA and 0.2%TEA additives. Flow rate: 2 mL/min; detection UV: 254 nm.

    Article Snippet: Acetonitrile (ACN), methanol (MeOH), ethanol (EtOH), n-heptane (hept), and isopropyl alcohol (IPA) of HPLC grade were obtained from VWR (Sugarland, Texas), and used as received.

    Techniques: Indirect Immunoperoxidase Assay, Flow Cytometry

    Chromatographic separation of (A) 18 SPMs (100 nM each) and (B) 5 deuterated internal standards (20 nM) covered by the method including (C) DHA derived D-series resolvins, (D) EPA derived E-series resolvins, (E) EPA and ARA derived lipoxins, DHA derived (F) protectins, and (G) maresins. Separation was carried out on an RP-18 column (2.1 × 150 mm, particle size 1.8 μm, pore size 9.5 nm) with a H 2 O/MeOH/ACN/HAc gradient.

    Journal: Frontiers in Pharmacology

    Article Title: Development of an Optimized LC-MS Method for the Detection of Specialized Pro-Resolving Mediators in Biological Samples

    doi: 10.3389/fphar.2019.00169

    Figure Lengend Snippet: Chromatographic separation of (A) 18 SPMs (100 nM each) and (B) 5 deuterated internal standards (20 nM) covered by the method including (C) DHA derived D-series resolvins, (D) EPA derived E-series resolvins, (E) EPA and ARA derived lipoxins, DHA derived (F) protectins, and (G) maresins. Separation was carried out on an RP-18 column (2.1 × 150 mm, particle size 1.8 μm, pore size 9.5 nm) with a H 2 O/MeOH/ACN/HAc gradient.

    Article Snippet: Acetonitrile (ACN), LC-MS grade methanol (MeOH) and acetic acid were obtained from Fisher Scientific (Schwerte, Germany).

    Techniques: Derivative Assay, Acetylene Reduction Assay, HAC Assay

    HPLC profiles of the fragmentation of LK by selected Saps. LK samples (1.5 μM) were digested with 0.03 μM of Sap1, −3, −4, −8, and −9 in the citrate buffer (pH 5.0) at 37°C for 24 hours. The reaction was stopped using pepstatin A (10 μM), followed by acidification with HCl (0.33 M). The samples were analyzed using HPLC on the Luna C18(2) 5 μm 4.6 × 250 mm column (Phenomenex) in a TFA water-ACN binary gradient system, as described in the Materials and Methods section. Arrows indicate the retention times of the Met-Lys-bradykinin (M) and des-Arg 1 -bradykinin (D) standards.

    Journal: BMC Microbiology

    Article Title: Kinin release from human kininogen by 10 aspartic proteases produced by pathogenic yeast Candida albicans

    doi: 10.1186/s12866-015-0394-8

    Figure Lengend Snippet: HPLC profiles of the fragmentation of LK by selected Saps. LK samples (1.5 μM) were digested with 0.03 μM of Sap1, −3, −4, −8, and −9 in the citrate buffer (pH 5.0) at 37°C for 24 hours. The reaction was stopped using pepstatin A (10 μM), followed by acidification with HCl (0.33 M). The samples were analyzed using HPLC on the Luna C18(2) 5 μm 4.6 × 250 mm column (Phenomenex) in a TFA water-ACN binary gradient system, as described in the Materials and Methods section. Arrows indicate the retention times of the Met-Lys-bradykinin (M) and des-Arg 1 -bradykinin (D) standards.

    Article Snippet: Briefly, the protein bands, which were visualized using colloidal staining with Coomassie Brilliant Blue dye [ ], were excised from the gel and washed twice with 50 mM (NH4 )HCO3 in 50% acetonitrile (ACN) (HPLC gradient-grade; Merck, Darmstadt, Germany) at both room temperature and 37°C in order to remove any dye, and then reduced using 10 mM dithiothreitol (DTT) for 60 minutes at 60°C, alkylated with 55 mM iodoacetamide for 45 minutes at room temperature in the dark, washed with 100 mM (NH4 )HCO3 , and dehydrated with ACN.

    Techniques: High Performance Liquid Chromatography

    Distribution of bradykinin, Met-Lys-bradykinin, and des-Arg 1 -bradykinin in the Sap-digested LK samples. LK samples (1.5 μM) were digested using 0.03 μM Sap in the citrate buffer (pH 5.0) at 37°C for 6 hours. After sequentially stopping the reaction using pepstatin A and HCl, the obtained peptide mixtures were separated on the Luna C18 column in a TFA water-ACN binary gradient system. The fractions were collected at the retention times that correspond to the bradykinin, Met-Lys-bradykinin, and des-Arg 1 -bradykinin standards, evaporated to dryness, and redissolved in the assay buffer of the ELISA kit in order to quantitatively determine the kinin concentrations. The corresponding fractions, obtained from the HPLC separation of intact (undigested) LK served as the controls, and the kinin concentrations, determined in these fractions are subtracted from those in the Sap-digested LK samples. The corrected amount of each of the three kinins is expressed relative to the maximum possible amount of all kinins (as calculated using the molarity of LK in the sample). Results, obtained from two independent experiments (two LK-digests independently analyzed by HPLC for each Sap), with three replicate ELISA measurements (in three different wells) for each fraction obtained during each HPLC separation, are presented as the mean values ± standard deviation. Asterisks denote the statistical significance of the difference between the kinin levels in the Sap-treated and undigested LK samples (t-Student test, p

    Journal: BMC Microbiology

    Article Title: Kinin release from human kininogen by 10 aspartic proteases produced by pathogenic yeast Candida albicans

    doi: 10.1186/s12866-015-0394-8

    Figure Lengend Snippet: Distribution of bradykinin, Met-Lys-bradykinin, and des-Arg 1 -bradykinin in the Sap-digested LK samples. LK samples (1.5 μM) were digested using 0.03 μM Sap in the citrate buffer (pH 5.0) at 37°C for 6 hours. After sequentially stopping the reaction using pepstatin A and HCl, the obtained peptide mixtures were separated on the Luna C18 column in a TFA water-ACN binary gradient system. The fractions were collected at the retention times that correspond to the bradykinin, Met-Lys-bradykinin, and des-Arg 1 -bradykinin standards, evaporated to dryness, and redissolved in the assay buffer of the ELISA kit in order to quantitatively determine the kinin concentrations. The corresponding fractions, obtained from the HPLC separation of intact (undigested) LK served as the controls, and the kinin concentrations, determined in these fractions are subtracted from those in the Sap-digested LK samples. The corrected amount of each of the three kinins is expressed relative to the maximum possible amount of all kinins (as calculated using the molarity of LK in the sample). Results, obtained from two independent experiments (two LK-digests independently analyzed by HPLC for each Sap), with three replicate ELISA measurements (in three different wells) for each fraction obtained during each HPLC separation, are presented as the mean values ± standard deviation. Asterisks denote the statistical significance of the difference between the kinin levels in the Sap-treated and undigested LK samples (t-Student test, p

    Article Snippet: Briefly, the protein bands, which were visualized using colloidal staining with Coomassie Brilliant Blue dye [ ], were excised from the gel and washed twice with 50 mM (NH4 )HCO3 in 50% acetonitrile (ACN) (HPLC gradient-grade; Merck, Darmstadt, Germany) at both room temperature and 37°C in order to remove any dye, and then reduced using 10 mM dithiothreitol (DTT) for 60 minutes at 60°C, alkylated with 55 mM iodoacetamide for 45 minutes at room temperature in the dark, washed with 100 mM (NH4 )HCO3 , and dehydrated with ACN.

    Techniques: Enzyme-linked Immunosorbent Assay, High Performance Liquid Chromatography, Standard Deviation

    HPLC/MS characteristics of the Sap-catalyzed cleavage of the HK-D4 peptide. Ten μM of the synthetic peptide HK-D4 (which has the ISLMKRPPGFSPFRSSRIGEIKEET amino acid sequence) were treated with recombinant Sap1–10 in citrate (50 mM) or phosphate buffers (25 mM) at the optimal pH for the general proteolytic activity [ 26 ] of each individual Sap (specified in the figure) at an enzyme:substrate molar ratio of 1:50 for 24 hours at 37°C. The reaction was stopped using HCl, and the samples were analyzed using reversed-phase HPLC on an Eurosil Bioselect 300–5 C-18 column (Knauer) in a TFA-water-ACN binary gradient system. The fractions, which were collected at the major absorbance peaks (215 nm), were evaporated and analyzed using ESI-MS/MS in order to determine their amino acid sequence.

    Journal: BMC Microbiology

    Article Title: Kinin release from human kininogen by 10 aspartic proteases produced by pathogenic yeast Candida albicans

    doi: 10.1186/s12866-015-0394-8

    Figure Lengend Snippet: HPLC/MS characteristics of the Sap-catalyzed cleavage of the HK-D4 peptide. Ten μM of the synthetic peptide HK-D4 (which has the ISLMKRPPGFSPFRSSRIGEIKEET amino acid sequence) were treated with recombinant Sap1–10 in citrate (50 mM) or phosphate buffers (25 mM) at the optimal pH for the general proteolytic activity [ 26 ] of each individual Sap (specified in the figure) at an enzyme:substrate molar ratio of 1:50 for 24 hours at 37°C. The reaction was stopped using HCl, and the samples were analyzed using reversed-phase HPLC on an Eurosil Bioselect 300–5 C-18 column (Knauer) in a TFA-water-ACN binary gradient system. The fractions, which were collected at the major absorbance peaks (215 nm), were evaporated and analyzed using ESI-MS/MS in order to determine their amino acid sequence.

    Article Snippet: Briefly, the protein bands, which were visualized using colloidal staining with Coomassie Brilliant Blue dye [ ], were excised from the gel and washed twice with 50 mM (NH4 )HCO3 in 50% acetonitrile (ACN) (HPLC gradient-grade; Merck, Darmstadt, Germany) at both room temperature and 37°C in order to remove any dye, and then reduced using 10 mM dithiothreitol (DTT) for 60 minutes at 60°C, alkylated with 55 mM iodoacetamide for 45 minutes at room temperature in the dark, washed with 100 mM (NH4 )HCO3 , and dehydrated with ACN.

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry, Sequencing, Recombinant, Activity Assay

    RP-HPLC analysis of PEGylated HM-3. Analysis was performed on a C18 column with water/ACN/TFA as eluent and a flow rate of 1 mL/min, and peaks were monitored at 220 nm. ( A ) ALD-mPEG 5k -HM-3 purity: 97.39%, T: 9.395 min; ( B ) ALD-mPEG 10k -HM-3 purity: 98.23%, T: 9.698 min; ( C ) SC-mPEG 10k -HM-3 purity: 97.02%, T: 9.883 min; ( D ) SC-mPEG 20k -HM-3 purity: 98.52%, T: 9.578 min.

    Journal: International Journal of Molecular Sciences

    Article Title: In Vivo Anti-Tumor Activity of Polypeptide HM-3 Modified by Different Polyethylene Glycols (PEG)

    doi: 10.3390/ijms12042650

    Figure Lengend Snippet: RP-HPLC analysis of PEGylated HM-3. Analysis was performed on a C18 column with water/ACN/TFA as eluent and a flow rate of 1 mL/min, and peaks were monitored at 220 nm. ( A ) ALD-mPEG 5k -HM-3 purity: 97.39%, T: 9.395 min; ( B ) ALD-mPEG 10k -HM-3 purity: 98.23%, T: 9.698 min; ( C ) SC-mPEG 10k -HM-3 purity: 97.02%, T: 9.883 min; ( D ) SC-mPEG 20k -HM-3 purity: 98.52%, T: 9.578 min.

    Article Snippet: Trifluoroacetate (TFA), acetonitrile (ACN), and sodium cyanoborohydride were purchased from TEDIA, ROE Scientific Inc., and Shanghai Darui Fine Chemicals Co. Ltd, respectively.

    Techniques: High Performance Liquid Chromatography, Flow Cytometry

    HPLC chromatograms: ( a ) condition 1 with Agilent XDB-C18 at an isocratic mobile phase of acetonitrile (ACN):water (0.7% trifluoroacetic acid (TFA), 5 mM heptafluorobutyric acid (HFBA)) = 2:98; ( b ) condition 2 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 2:98; ( c ) condition 3 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 9:91; ( d ) after approximately 48 hours of repeated measurements with condition 3.

    Journal: Foods

    Article Title: New Analytical Approach for the Determination of Calcium Phosphate Dibasic and Tribasic in Processed Food by Comparison of Ion Chromatography with High-Performance Liquid Chromatography

    doi: 10.3390/foods9030248

    Figure Lengend Snippet: HPLC chromatograms: ( a ) condition 1 with Agilent XDB-C18 at an isocratic mobile phase of acetonitrile (ACN):water (0.7% trifluoroacetic acid (TFA), 5 mM heptafluorobutyric acid (HFBA)) = 2:98; ( b ) condition 2 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 2:98; ( c ) condition 3 with YMC Triart-C8 at an isocratic mobile phase of ACN:water (0.7% TFA, 5 mM HFBA) = 9:91; ( d ) after approximately 48 hours of repeated measurements with condition 3.

    Article Snippet: Chemicals and Reagents Hexanes, HPLC grade water, and acetonitrile (ACN) were purchased from Honeywell Burdick and Jackson (Ulsan, Korea), and hydrochloric acid and calcium chloride anhydrous were obtained from Duksan Pure Chemical (Ansan, Korea).

    Techniques: High Performance Liquid Chromatography