aci i New England Biolabs Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    New England Biolabs aci i
    PCR-RFLP of a gltA gene fragment using the restriction endonuclease <t>Aci</t> I. Lanes 1, 4 and 19 show 100 BP Ladder; Lane 2, Mt Lion L27-96; Lane 3, Mt Lion L42-94; Lane 5, Mt Lion L-39-97; Lane 6, Mt Lion FM98061; Lane 7, Bobcat L08-96; Lane 8, Bobcat L17-96; Lane 9, Bobcat DS08; Lane 10, Bobcat L10-97; Lane 11, Bobcat L11-97; Lane 12, Bobcat SC443; lane 13, Bobcat DS507; Lane 14, B . henselae Type I; Lane 15, B . henselae Type II; Lane 16, B . clarridgeiae ; Lane 17, B . koehlerae ; Lane18, B . bovis (“weissii” isolate).
    Aci I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 227 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aci i/product/New England Biolabs
    Average 95 stars, based on 227 article reviews
    Price from $9.99 to $1999.99
    aci i - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    90
    Qiagen minielute pcr purification kits
    PCR-RFLP of a gltA gene fragment using the restriction endonuclease <t>Aci</t> I. Lanes 1, 4 and 19 show 100 BP Ladder; Lane 2, Mt Lion L27-96; Lane 3, Mt Lion L42-94; Lane 5, Mt Lion L-39-97; Lane 6, Mt Lion FM98061; Lane 7, Bobcat L08-96; Lane 8, Bobcat L17-96; Lane 9, Bobcat DS08; Lane 10, Bobcat L10-97; Lane 11, Bobcat L11-97; Lane 12, Bobcat SC443; lane 13, Bobcat DS507; Lane 14, B . henselae Type I; Lane 15, B . henselae Type II; Lane 16, B . clarridgeiae ; Lane 17, B . koehlerae ; Lane18, B . bovis (“weissii” isolate).
    Minielute Pcr Purification Kits, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/minielute pcr purification kits/product/Qiagen
    Average 90 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    minielute pcr purification kits - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    77
    New England Biolabs aci
    PCR-RFLP of a gltA gene fragment using the restriction endonuclease <t>Aci</t> I. Lanes 1, 4 and 19 show 100 BP Ladder; Lane 2, Mt Lion L27-96; Lane 3, Mt Lion L42-94; Lane 5, Mt Lion L-39-97; Lane 6, Mt Lion FM98061; Lane 7, Bobcat L08-96; Lane 8, Bobcat L17-96; Lane 9, Bobcat DS08; Lane 10, Bobcat L10-97; Lane 11, Bobcat L11-97; Lane 12, Bobcat SC443; lane 13, Bobcat DS507; Lane 14, B . henselae Type I; Lane 15, B . henselae Type II; Lane 16, B . clarridgeiae ; Lane 17, B . koehlerae ; Lane18, B . bovis (“weissii” isolate).
    Aci, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 77/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aci/product/New England Biolabs
    Average 77 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    aci - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    95
    New England Biolabs endonuclease aci i
    PCR-RFLP of a gltA gene fragment using the restriction endonuclease <t>Aci</t> I. Lanes 1, 4 and 19 show 100 BP Ladder; Lane 2, Mt Lion L27-96; Lane 3, Mt Lion L42-94; Lane 5, Mt Lion L-39-97; Lane 6, Mt Lion FM98061; Lane 7, Bobcat L08-96; Lane 8, Bobcat L17-96; Lane 9, Bobcat DS08; Lane 10, Bobcat L10-97; Lane 11, Bobcat L11-97; Lane 12, Bobcat SC443; lane 13, Bobcat DS507; Lane 14, B . henselae Type I; Lane 15, B . henselae Type II; Lane 16, B . clarridgeiae ; Lane 17, B . koehlerae ; Lane18, B . bovis (“weissii” isolate).
    Endonuclease Aci I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/endonuclease aci i/product/New England Biolabs
    Average 95 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    endonuclease aci i - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    99
    New England Biolabs aci i restriction enzyme
    Fig. 1. Demethylation of tissue-specific gene promoter sequences in IVF (A and B) or cloned (C and D) bovine embryos. ( A and C ) The epidermal cytokeratin gene sequence (166 bp, four <t>Aci</t> I recognition sites). ( B and D ) The β-lactoglobulin gene sequence (110 bp, three Taq I sites). The number of embryos used in each lane was ∼200 for matured oocytes, ∼100 for one-cell eggs, ∼30 of four- to eight-cell embryos, ∼10 for morulae and about six for blastocysts. Both target regions consist of unique sequences with no interrupting repeat such as transposons. Photographs, negative images. Arrows, position of intact PCR products. M, <t>DNA</t> size marker (bp); X, intact, undigested PCR products; O, enzyme-treated PCR products; donor, fetal bovine fibroblasts; 4/8c, four- to eight-cell embryos; mor, morulae; bl, blastocysts.
    Aci I Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aci i restriction enzyme/product/New England Biolabs
    Average 99 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    aci i restriction enzyme - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    95
    New England Biolabs hpa ii
    The Klf1 exon 2 CGI is unmethylated during development. A ) Schematic of the mouse Klf1 gene across exon 2 and the flanking introns shows salient restriction enzyme sites within the region and a 1.1-kbp cDNA probe used for Southern blot. B ) Genomic <t>DNA</t> samples from whole testis between P5 and P45 (lanes 1–7, left and center panels) and P45 spleen (lane 8). DNAs were purified and double-digested with Eco RI and Bam HI restriction enzymes to liberate the 1.85-kb genomic fragment containing the Klf1 exon 2 CGI. Thereafter, the DNA samples were further digested with <t>Hpa</t> II (left panel) or not digested (center panel) and then Southern blotted using the 1.1-kb probe shown in A . In addition, several samples were methylated using Hpa II -methyltransferase then digested with Hpa II (right panel) and Southern blotted. Because of the total number of samples in this experiment, three Southern blots were generated, and the relevant lanes were used to form composite panels. Data for the P5–P15 lanes (break in Southern blots) were obtained from separate Southern blots. Arrowheads in A , locations of Hpa II restriction sites; FL in B , full-length 1.85-kb Eco RI - Bam HI fragments that are visible in the center and right panels; Spl, spleen.
    Hpa Ii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 914 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hpa ii/product/New England Biolabs
    Average 95 stars, based on 914 article reviews
    Price from $9.99 to $1999.99
    hpa ii - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    78
    New England Biolabs aci 1
    The Klf1 exon 2 CGI is unmethylated during development. A ) Schematic of the mouse Klf1 gene across exon 2 and the flanking introns shows salient restriction enzyme sites within the region and a 1.1-kbp cDNA probe used for Southern blot. B ) Genomic <t>DNA</t> samples from whole testis between P5 and P45 (lanes 1–7, left and center panels) and P45 spleen (lane 8). DNAs were purified and double-digested with Eco RI and Bam HI restriction enzymes to liberate the 1.85-kb genomic fragment containing the Klf1 exon 2 CGI. Thereafter, the DNA samples were further digested with <t>Hpa</t> II (left panel) or not digested (center panel) and then Southern blotted using the 1.1-kb probe shown in A . In addition, several samples were methylated using Hpa II -methyltransferase then digested with Hpa II (right panel) and Southern blotted. Because of the total number of samples in this experiment, three Southern blots were generated, and the relevant lanes were used to form composite panels. Data for the P5–P15 lanes (break in Southern blots) were obtained from separate Southern blots. Arrowheads in A , locations of Hpa II restriction sites; FL in B , full-length 1.85-kb Eco RI - Bam HI fragments that are visible in the center and right panels; Spl, spleen.
    Aci 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aci 1/product/New England Biolabs
    Average 78 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    aci 1 - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    99
    New England Biolabs restriction endonuclease aci i
    The Klf1 exon 2 CGI is unmethylated during development. A ) Schematic of the mouse Klf1 gene across exon 2 and the flanking introns shows salient restriction enzyme sites within the region and a 1.1-kbp cDNA probe used for Southern blot. B ) Genomic <t>DNA</t> samples from whole testis between P5 and P45 (lanes 1–7, left and center panels) and P45 spleen (lane 8). DNAs were purified and double-digested with Eco RI and Bam HI restriction enzymes to liberate the 1.85-kb genomic fragment containing the Klf1 exon 2 CGI. Thereafter, the DNA samples were further digested with <t>Hpa</t> II (left panel) or not digested (center panel) and then Southern blotted using the 1.1-kb probe shown in A . In addition, several samples were methylated using Hpa II -methyltransferase then digested with Hpa II (right panel) and Southern blotted. Because of the total number of samples in this experiment, three Southern blots were generated, and the relevant lanes were used to form composite panels. Data for the P5–P15 lanes (break in Southern blots) were obtained from separate Southern blots. Arrowheads in A , locations of Hpa II restriction sites; FL in B , full-length 1.85-kb Eco RI - Bam HI fragments that are visible in the center and right panels; Spl, spleen.
    Restriction Endonuclease Aci I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction endonuclease aci i/product/New England Biolabs
    Average 99 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    restriction endonuclease aci i - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    81
    New England Biolabs aci i restriction enzymes
    The Klf1 exon 2 CGI is unmethylated during development. A ) Schematic of the mouse Klf1 gene across exon 2 and the flanking introns shows salient restriction enzyme sites within the region and a 1.1-kbp cDNA probe used for Southern blot. B ) Genomic <t>DNA</t> samples from whole testis between P5 and P45 (lanes 1–7, left and center panels) and P45 spleen (lane 8). DNAs were purified and double-digested with Eco RI and Bam HI restriction enzymes to liberate the 1.85-kb genomic fragment containing the Klf1 exon 2 CGI. Thereafter, the DNA samples were further digested with <t>Hpa</t> II (left panel) or not digested (center panel) and then Southern blotted using the 1.1-kb probe shown in A . In addition, several samples were methylated using Hpa II -methyltransferase then digested with Hpa II (right panel) and Southern blotted. Because of the total number of samples in this experiment, three Southern blots were generated, and the relevant lanes were used to form composite panels. Data for the P5–P15 lanes (break in Southern blots) were obtained from separate Southern blots. Arrowheads in A , locations of Hpa II restriction sites; FL in B , full-length 1.85-kb Eco RI - Bam HI fragments that are visible in the center and right panels; Spl, spleen.
    Aci I Restriction Enzymes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 81/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aci i restriction enzymes/product/New England Biolabs
    Average 81 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    aci i restriction enzymes - by Bioz Stars, 2020-02
    81/100 stars
      Buy from Supplier

    76
    New England Biolabs acy atp
    The Klf1 exon 2 CGI is unmethylated during development. A ) Schematic of the mouse Klf1 gene across exon 2 and the flanking introns shows salient restriction enzyme sites within the region and a 1.1-kbp cDNA probe used for Southern blot. B ) Genomic <t>DNA</t> samples from whole testis between P5 and P45 (lanes 1–7, left and center panels) and P45 spleen (lane 8). DNAs were purified and double-digested with Eco RI and Bam HI restriction enzymes to liberate the 1.85-kb genomic fragment containing the Klf1 exon 2 CGI. Thereafter, the DNA samples were further digested with <t>Hpa</t> II (left panel) or not digested (center panel) and then Southern blotted using the 1.1-kb probe shown in A . In addition, several samples were methylated using Hpa II -methyltransferase then digested with Hpa II (right panel) and Southern blotted. Because of the total number of samples in this experiment, three Southern blots were generated, and the relevant lanes were used to form composite panels. Data for the P5–P15 lanes (break in Southern blots) were obtained from separate Southern blots. Arrowheads in A , locations of Hpa II restriction sites; FL in B , full-length 1.85-kb Eco RI - Bam HI fragments that are visible in the center and right panels; Spl, spleen.
    Acy Atp, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 76/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acy atp/product/New England Biolabs
    Average 76 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    acy atp - by Bioz Stars, 2020-02
    76/100 stars
      Buy from Supplier

    81
    Thermo Fisher aci i
    The Klf1 exon 2 CGI is unmethylated during development. A ) Schematic of the mouse Klf1 gene across exon 2 and the flanking introns shows salient restriction enzyme sites within the region and a 1.1-kbp cDNA probe used for Southern blot. B ) Genomic <t>DNA</t> samples from whole testis between P5 and P45 (lanes 1–7, left and center panels) and P45 spleen (lane 8). DNAs were purified and double-digested with Eco RI and Bam HI restriction enzymes to liberate the 1.85-kb genomic fragment containing the Klf1 exon 2 CGI. Thereafter, the DNA samples were further digested with <t>Hpa</t> II (left panel) or not digested (center panel) and then Southern blotted using the 1.1-kb probe shown in A . In addition, several samples were methylated using Hpa II -methyltransferase then digested with Hpa II (right panel) and Southern blotted. Because of the total number of samples in this experiment, three Southern blots were generated, and the relevant lanes were used to form composite panels. Data for the P5–P15 lanes (break in Southern blots) were obtained from separate Southern blots. Arrowheads in A , locations of Hpa II restriction sites; FL in B , full-length 1.85-kb Eco RI - Bam HI fragments that are visible in the center and right panels; Spl, spleen.
    Aci I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aci i/product/Thermo Fisher
    Average 81 stars, based on 37 article reviews
    Price from $9.99 to $1999.99
    aci i - by Bioz Stars, 2020-02
    81/100 stars
      Buy from Supplier

    95
    New England Biolabs alu i
    The Klf1 exon 2 CGI is unmethylated during development. A ) Schematic of the mouse Klf1 gene across exon 2 and the flanking introns shows salient restriction enzyme sites within the region and a 1.1-kbp cDNA probe used for Southern blot. B ) Genomic <t>DNA</t> samples from whole testis between P5 and P45 (lanes 1–7, left and center panels) and P45 spleen (lane 8). DNAs were purified and double-digested with Eco RI and Bam HI restriction enzymes to liberate the 1.85-kb genomic fragment containing the Klf1 exon 2 CGI. Thereafter, the DNA samples were further digested with <t>Hpa</t> II (left panel) or not digested (center panel) and then Southern blotted using the 1.1-kb probe shown in A . In addition, several samples were methylated using Hpa II -methyltransferase then digested with Hpa II (right panel) and Southern blotted. Because of the total number of samples in this experiment, three Southern blots were generated, and the relevant lanes were used to form composite panels. Data for the P5–P15 lanes (break in Southern blots) were obtained from separate Southern blots. Arrowheads in A , locations of Hpa II restriction sites; FL in B , full-length 1.85-kb Eco RI - Bam HI fragments that are visible in the center and right panels; Spl, spleen.
    Alu I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 605 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alu i/product/New England Biolabs
    Average 95 stars, based on 605 article reviews
    Price from $9.99 to $1999.99
    alu i - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    95
    New England Biolabs hpych4 iv
    The Klf1 exon 2 CGI is unmethylated during development. A ) Schematic of the mouse Klf1 gene across exon 2 and the flanking introns shows salient restriction enzyme sites within the region and a 1.1-kbp cDNA probe used for Southern blot. B ) Genomic <t>DNA</t> samples from whole testis between P5 and P45 (lanes 1–7, left and center panels) and P45 spleen (lane 8). DNAs were purified and double-digested with Eco RI and Bam HI restriction enzymes to liberate the 1.85-kb genomic fragment containing the Klf1 exon 2 CGI. Thereafter, the DNA samples were further digested with <t>Hpa</t> II (left panel) or not digested (center panel) and then Southern blotted using the 1.1-kb probe shown in A . In addition, several samples were methylated using Hpa II -methyltransferase then digested with Hpa II (right panel) and Southern blotted. Because of the total number of samples in this experiment, three Southern blots were generated, and the relevant lanes were used to form composite panels. Data for the P5–P15 lanes (break in Southern blots) were obtained from separate Southern blots. Arrowheads in A , locations of Hpa II restriction sites; FL in B , full-length 1.85-kb Eco RI - Bam HI fragments that are visible in the center and right panels; Spl, spleen.
    Hpych4 Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hpych4 iv/product/New England Biolabs
    Average 95 stars, based on 78 article reviews
    Price from $9.99 to $1999.99
    hpych4 iv - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    95
    New England Biolabs gibson assembly cloning kit
    The Klf1 exon 2 CGI is unmethylated during development. A ) Schematic of the mouse Klf1 gene across exon 2 and the flanking introns shows salient restriction enzyme sites within the region and a 1.1-kbp cDNA probe used for Southern blot. B ) Genomic <t>DNA</t> samples from whole testis between P5 and P45 (lanes 1–7, left and center panels) and P45 spleen (lane 8). DNAs were purified and double-digested with Eco RI and Bam HI restriction enzymes to liberate the 1.85-kb genomic fragment containing the Klf1 exon 2 CGI. Thereafter, the DNA samples were further digested with <t>Hpa</t> II (left panel) or not digested (center panel) and then Southern blotted using the 1.1-kb probe shown in A . In addition, several samples were methylated using Hpa II -methyltransferase then digested with Hpa II (right panel) and Southern blotted. Because of the total number of samples in this experiment, three Southern blots were generated, and the relevant lanes were used to form composite panels. Data for the P5–P15 lanes (break in Southern blots) were obtained from separate Southern blots. Arrowheads in A , locations of Hpa II restriction sites; FL in B , full-length 1.85-kb Eco RI - Bam HI fragments that are visible in the center and right panels; Spl, spleen.
    Gibson Assembly Cloning Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 2170 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gibson assembly cloning kit/product/New England Biolabs
    Average 95 stars, based on 2170 article reviews
    Price from $9.99 to $1999.99
    gibson assembly cloning kit - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    95
    New England Biolabs bsm fi
    The Klf1 exon 2 CGI is unmethylated during development. A ) Schematic of the mouse Klf1 gene across exon 2 and the flanking introns shows salient restriction enzyme sites within the region and a 1.1-kbp cDNA probe used for Southern blot. B ) Genomic <t>DNA</t> samples from whole testis between P5 and P45 (lanes 1–7, left and center panels) and P45 spleen (lane 8). DNAs were purified and double-digested with Eco RI and Bam HI restriction enzymes to liberate the 1.85-kb genomic fragment containing the Klf1 exon 2 CGI. Thereafter, the DNA samples were further digested with <t>Hpa</t> II (left panel) or not digested (center panel) and then Southern blotted using the 1.1-kb probe shown in A . In addition, several samples were methylated using Hpa II -methyltransferase then digested with Hpa II (right panel) and Southern blotted. Because of the total number of samples in this experiment, three Southern blots were generated, and the relevant lanes were used to form composite panels. Data for the P5–P15 lanes (break in Southern blots) were obtained from separate Southern blots. Arrowheads in A , locations of Hpa II restriction sites; FL in B , full-length 1.85-kb Eco RI - Bam HI fragments that are visible in the center and right panels; Spl, spleen.
    Bsm Fi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bsm fi/product/New England Biolabs
    Average 95 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    bsm fi - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    93
    New England Biolabs acl i
    The Klf1 exon 2 CGI is unmethylated during development. A ) Schematic of the mouse Klf1 gene across exon 2 and the flanking introns shows salient restriction enzyme sites within the region and a 1.1-kbp cDNA probe used for Southern blot. B ) Genomic <t>DNA</t> samples from whole testis between P5 and P45 (lanes 1–7, left and center panels) and P45 spleen (lane 8). DNAs were purified and double-digested with Eco RI and Bam HI restriction enzymes to liberate the 1.85-kb genomic fragment containing the Klf1 exon 2 CGI. Thereafter, the DNA samples were further digested with <t>Hpa</t> II (left panel) or not digested (center panel) and then Southern blotted using the 1.1-kb probe shown in A . In addition, several samples were methylated using Hpa II -methyltransferase then digested with Hpa II (right panel) and Southern blotted. Because of the total number of samples in this experiment, three Southern blots were generated, and the relevant lanes were used to form composite panels. Data for the P5–P15 lanes (break in Southern blots) were obtained from separate Southern blots. Arrowheads in A , locations of Hpa II restriction sites; FL in B , full-length 1.85-kb Eco RI - Bam HI fragments that are visible in the center and right panels; Spl, spleen.
    Acl I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acl i/product/New England Biolabs
    Average 93 stars, based on 47 article reviews
    Price from $9.99 to $1999.99
    acl i - by Bioz Stars, 2020-02
    93/100 stars
      Buy from Supplier

    95
    New England Biolabs msp i
    The Klf1 exon 2 CGI is unmethylated during development. A ) Schematic of the mouse Klf1 gene across exon 2 and the flanking introns shows salient restriction enzyme sites within the region and a 1.1-kbp cDNA probe used for Southern blot. B ) Genomic <t>DNA</t> samples from whole testis between P5 and P45 (lanes 1–7, left and center panels) and P45 spleen (lane 8). DNAs were purified and double-digested with Eco RI and Bam HI restriction enzymes to liberate the 1.85-kb genomic fragment containing the Klf1 exon 2 CGI. Thereafter, the DNA samples were further digested with <t>Hpa</t> II (left panel) or not digested (center panel) and then Southern blotted using the 1.1-kb probe shown in A . In addition, several samples were methylated using Hpa II -methyltransferase then digested with Hpa II (right panel) and Southern blotted. Because of the total number of samples in this experiment, three Southern blots were generated, and the relevant lanes were used to form composite panels. Data for the P5–P15 lanes (break in Southern blots) were obtained from separate Southern blots. Arrowheads in A , locations of Hpa II restriction sites; FL in B , full-length 1.85-kb Eco RI - Bam HI fragments that are visible in the center and right panels; Spl, spleen.
    Msp I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1029 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/msp i/product/New England Biolabs
    Average 95 stars, based on 1029 article reviews
    Price from $9.99 to $1999.99
    msp i - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    95
    New England Biolabs ecor i hf
    The Klf1 exon 2 CGI is unmethylated during development. A ) Schematic of the mouse Klf1 gene across exon 2 and the flanking introns shows salient restriction enzyme sites within the region and a 1.1-kbp cDNA probe used for Southern blot. B ) Genomic <t>DNA</t> samples from whole testis between P5 and P45 (lanes 1–7, left and center panels) and P45 spleen (lane 8). DNAs were purified and double-digested with Eco RI and Bam HI restriction enzymes to liberate the 1.85-kb genomic fragment containing the Klf1 exon 2 CGI. Thereafter, the DNA samples were further digested with <t>Hpa</t> II (left panel) or not digested (center panel) and then Southern blotted using the 1.1-kb probe shown in A . In addition, several samples were methylated using Hpa II -methyltransferase then digested with Hpa II (right panel) and Southern blotted. Because of the total number of samples in this experiment, three Southern blots were generated, and the relevant lanes were used to form composite panels. Data for the P5–P15 lanes (break in Southern blots) were obtained from separate Southern blots. Arrowheads in A , locations of Hpa II restriction sites; FL in B , full-length 1.85-kb Eco RI - Bam HI fragments that are visible in the center and right panels; Spl, spleen.
    Ecor I Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecor i hf/product/New England Biolabs
    Average 95 stars, based on 63 article reviews
    Price from $9.99 to $1999.99
    ecor i hf - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    95
    New England Biolabs t4 dna polymerase
    Effect of MnCl 2 on the 3′ to 5′ exonuclease activities of UL30 and <t>T4</t> DNA polymerases. ( A ) Autoradiogram of the reaction products with UL30 DNA polymerase. Reactions were performed as described in Materials and Methods with 20 nM platinated substrates D or E and 20 nM UL30 and either 10 mM MgCl 2 or 4 mM MnCl 2 for the times indicated. Lanes 1–10, substrate D. Lanes 11–20, substrate E. ( B ) Autoradiogram of the reaction products with T4 and UL30 DNA polymerases. Reactions were performed as described in Materials and Methods with 20 nM platinated substrate D and 20 nM UL30 or T4 polymerase and either 10 mM MgCl 2 or 4 mM MnCl 2 for the times indicated.
    T4 Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 6750 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna polymerase/product/New England Biolabs
    Average 95 stars, based on 6750 article reviews
    Price from $9.99 to $1999.99
    t4 dna polymerase - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    95
    New England Biolabs nla iii
    <t>Nla</t> <t>III</t> RLFP analysis of PCR-amplified crmB fragments from DNA of CPV isolates from human and animals. Nla III digest fragments after PCR amplification of OPV85 (human) (lane 1), OPV88/L (cat) (lane 2), OPV88/H (cat) (lane 3), OPV89/1 (cat)-M5 (lane 4), OPV89/2 (cat) (lane 5), OPV89/3 (cat) (lane 6), OPV89/4 (cat)-M6 (lane 7), OPV89/5 (cat)-M7 (lane 8), OPV90/1 (cat)-M8 (lane 9), OPV90/2 (human) (lane 10), OPV90/4 (dog) (lane 11), OPV90/5 (cat)-M9 (lane 12), OPV91/1 (cat) (lane 13), OPV91/2 (human) (lane 14), OPV91/3 (cow) (lane 15), CATPOX3 (lane 16), CATPOX5 (lane 17), RAT Moscow (rat) (lane 18), EP-1 (elephant) (lane 19), EP-2 (elephant)-M1 (lane 20), EP-3 (elephant) (lane 21), EP-4 (elephant) (lane 22), EP-5 (elephant) (lane 23), CPV BRT-Atlanta (lane 24), and CPV BRT-Munich (lane 25) are shown.
    Nla Iii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 642 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nla iii/product/New England Biolabs
    Average 95 stars, based on 642 article reviews
    Price from $9.99 to $1999.99
    nla iii - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    95
    New England Biolabs hpy 188iii
    <t>Nla</t> <t>III</t> RLFP analysis of PCR-amplified crmB fragments from DNA of CPV isolates from human and animals. Nla III digest fragments after PCR amplification of OPV85 (human) (lane 1), OPV88/L (cat) (lane 2), OPV88/H (cat) (lane 3), OPV89/1 (cat)-M5 (lane 4), OPV89/2 (cat) (lane 5), OPV89/3 (cat) (lane 6), OPV89/4 (cat)-M6 (lane 7), OPV89/5 (cat)-M7 (lane 8), OPV90/1 (cat)-M8 (lane 9), OPV90/2 (human) (lane 10), OPV90/4 (dog) (lane 11), OPV90/5 (cat)-M9 (lane 12), OPV91/1 (cat) (lane 13), OPV91/2 (human) (lane 14), OPV91/3 (cow) (lane 15), CATPOX3 (lane 16), CATPOX5 (lane 17), RAT Moscow (rat) (lane 18), EP-1 (elephant) (lane 19), EP-2 (elephant)-M1 (lane 20), EP-3 (elephant) (lane 21), EP-4 (elephant) (lane 22), EP-5 (elephant) (lane 23), CPV BRT-Atlanta (lane 24), and CPV BRT-Munich (lane 25) are shown.
    Hpy 188iii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hpy 188iii/product/New England Biolabs
    Average 95 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    hpy 188iii - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    95
    New England Biolabs λ dna
    Agarose-gel analysis of tagmentation products of phage-λ DNA analyzed on ( A ) high-resolution and ( C ) low-resolution (i.e. mini) agarose gels. Size-distribution fits for the high-resolution gel ( B ) and mini gel ( D ) of the same tagmented <t>λ-DNA</t> sample subjected to increasing numbers of PCR-amplification cycles (lanes 3–5 in (A), lanes 2–4 in (C)): 8 cycles (top plots), 14 cycles (middle plots) and 20 cycles (bottom plots) in both (B) and (D). Vertical dashed lines (light red in (B), (D)) give the positions of maxima in the discrete molecular-weight ladder (blue ROI in (A), (C)).
    λ Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 542 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/λ dna/product/New England Biolabs
    Average 95 stars, based on 542 article reviews
    Price from $9.99 to $1999.99
    λ dna - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    aci  (Promega)
    77
    Promega aci
    Agarose-gel analysis of tagmentation products of phage-λ DNA analyzed on ( A ) high-resolution and ( C ) low-resolution (i.e. mini) agarose gels. Size-distribution fits for the high-resolution gel ( B ) and mini gel ( D ) of the same tagmented <t>λ-DNA</t> sample subjected to increasing numbers of PCR-amplification cycles (lanes 3–5 in (A), lanes 2–4 in (C)): 8 cycles (top plots), 14 cycles (middle plots) and 20 cycles (bottom plots) in both (B) and (D). Vertical dashed lines (light red in (B), (D)) give the positions of maxima in the discrete molecular-weight ladder (blue ROI in (A), (C)).
    Aci, supplied by Promega, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aci/product/Promega
    Average 77 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    aci - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    94
    New England Biolabs mly i
    Agarose-gel analysis of tagmentation products of phage-λ DNA analyzed on ( A ) high-resolution and ( C ) low-resolution (i.e. mini) agarose gels. Size-distribution fits for the high-resolution gel ( B ) and mini gel ( D ) of the same tagmented <t>λ-DNA</t> sample subjected to increasing numbers of PCR-amplification cycles (lanes 3–5 in (A), lanes 2–4 in (C)): 8 cycles (top plots), 14 cycles (middle plots) and 20 cycles (bottom plots) in both (B) and (D). Vertical dashed lines (light red in (B), (D)) give the positions of maxima in the discrete molecular-weight ladder (blue ROI in (A), (C)).
    Mly I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mly i/product/New England Biolabs
    Average 94 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    mly i - by Bioz Stars, 2020-02
    94/100 stars
      Buy from Supplier

    87
    Bionexus Inc hilo ladder
    Agarose-gel analysis of tagmentation products of phage-λ DNA analyzed on ( A ) high-resolution and ( C ) low-resolution (i.e. mini) agarose gels. Size-distribution fits for the high-resolution gel ( B ) and mini gel ( D ) of the same tagmented <t>λ-DNA</t> sample subjected to increasing numbers of PCR-amplification cycles (lanes 3–5 in (A), lanes 2–4 in (C)): 8 cycles (top plots), 14 cycles (middle plots) and 20 cycles (bottom plots) in both (B) and (D). Vertical dashed lines (light red in (B), (D)) give the positions of maxima in the discrete molecular-weight ladder (blue ROI in (A), (C)).
    Hilo Ladder, supplied by Bionexus Inc, used in various techniques. Bioz Stars score: 87/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hilo ladder/product/Bionexus Inc
    Average 87 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    hilo ladder - by Bioz Stars, 2020-02
    87/100 stars
      Buy from Supplier

    95
    New England Biolabs sau 3ai
    Agarose-gel analysis of tagmentation products of phage-λ DNA analyzed on ( A ) high-resolution and ( C ) low-resolution (i.e. mini) agarose gels. Size-distribution fits for the high-resolution gel ( B ) and mini gel ( D ) of the same tagmented <t>λ-DNA</t> sample subjected to increasing numbers of PCR-amplification cycles (lanes 3–5 in (A), lanes 2–4 in (C)): 8 cycles (top plots), 14 cycles (middle plots) and 20 cycles (bottom plots) in both (B) and (D). Vertical dashed lines (light red in (B), (D)) give the positions of maxima in the discrete molecular-weight ladder (blue ROI in (A), (C)).
    Sau 3ai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sau 3ai/product/New England Biolabs
    Average 95 stars, based on 153 article reviews
    Price from $9.99 to $1999.99
    sau 3ai - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    95
    New England Biolabs t4 dna ligase
    Agarose-gel analysis of tagmentation products of phage-λ DNA analyzed on ( A ) high-resolution and ( C ) low-resolution (i.e. mini) agarose gels. Size-distribution fits for the high-resolution gel ( B ) and mini gel ( D ) of the same tagmented <t>λ-DNA</t> sample subjected to increasing numbers of PCR-amplification cycles (lanes 3–5 in (A), lanes 2–4 in (C)): 8 cycles (top plots), 14 cycles (middle plots) and 20 cycles (bottom plots) in both (B) and (D). Vertical dashed lines (light red in (B), (D)) give the positions of maxima in the discrete molecular-weight ladder (blue ROI in (A), (C)).
    T4 Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 38631 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna ligase/product/New England Biolabs
    Average 95 stars, based on 38631 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    95
    New England Biolabs t4 polynucleotide kinase
    Agarose-gel analysis of tagmentation products of phage-λ DNA analyzed on ( A ) high-resolution and ( C ) low-resolution (i.e. mini) agarose gels. Size-distribution fits for the high-resolution gel ( B ) and mini gel ( D ) of the same tagmented <t>λ-DNA</t> sample subjected to increasing numbers of PCR-amplification cycles (lanes 3–5 in (A), lanes 2–4 in (C)): 8 cycles (top plots), 14 cycles (middle plots) and 20 cycles (bottom plots) in both (B) and (D). Vertical dashed lines (light red in (B), (D)) give the positions of maxima in the discrete molecular-weight ladder (blue ROI in (A), (C)).
    T4 Polynucleotide Kinase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 24211 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 polynucleotide kinase/product/New England Biolabs
    Average 95 stars, based on 24211 article reviews
    Price from $9.99 to $1999.99
    t4 polynucleotide kinase - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    95
    New England Biolabs xba i
    Agarose-gel analysis of tagmentation products of phage-λ DNA analyzed on ( A ) high-resolution and ( C ) low-resolution (i.e. mini) agarose gels. Size-distribution fits for the high-resolution gel ( B ) and mini gel ( D ) of the same tagmented <t>λ-DNA</t> sample subjected to increasing numbers of PCR-amplification cycles (lanes 3–5 in (A), lanes 2–4 in (C)): 8 cycles (top plots), 14 cycles (middle plots) and 20 cycles (bottom plots) in both (B) and (D). Vertical dashed lines (light red in (B), (D)) give the positions of maxima in the discrete molecular-weight ladder (blue ROI in (A), (C)).
    Xba I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 2655 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xba i/product/New England Biolabs
    Average 95 stars, based on 2655 article reviews
    Price from $9.99 to $1999.99
    xba i - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    90
    Thermo Fisher eco 72i
    Agarose-gel analysis of tagmentation products of phage-λ DNA analyzed on ( A ) high-resolution and ( C ) low-resolution (i.e. mini) agarose gels. Size-distribution fits for the high-resolution gel ( B ) and mini gel ( D ) of the same tagmented <t>λ-DNA</t> sample subjected to increasing numbers of PCR-amplification cycles (lanes 3–5 in (A), lanes 2–4 in (C)): 8 cycles (top plots), 14 cycles (middle plots) and 20 cycles (bottom plots) in both (B) and (D). Vertical dashed lines (light red in (B), (D)) give the positions of maxima in the discrete molecular-weight ladder (blue ROI in (A), (C)).
    Eco 72i, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eco 72i/product/Thermo Fisher
    Average 90 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    eco 72i - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    95
    New England Biolabs mse i
    Different fingerprinting profiles obtained by digesting the <t>tet</t> (M) amplicons from 64 tet (M)-positive pneumococci with four endonucleases. Lane M, molecular size marker (100-bp ladder). Lane A, undigested tet (M) amplicon. Lanes 1a to 1e, different Aci I profiles ( Aci I 1 to Aci I 5 ). Lanes 2a and 2b, different Rsa I profiles ( Rsa I 1 and Rsa I 2 ). Lane 3, <t>Mse</t> I profile. Lane 4, Taq I profile.
    Mse I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1003 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mse i/product/New England Biolabs
    Average 95 stars, based on 1003 article reviews
    Price from $9.99 to $1999.99
    mse i - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    Image Search Results


    PCR-RFLP of a gltA gene fragment using the restriction endonuclease Aci I. Lanes 1, 4 and 19 show 100 BP Ladder; Lane 2, Mt Lion L27-96; Lane 3, Mt Lion L42-94; Lane 5, Mt Lion L-39-97; Lane 6, Mt Lion FM98061; Lane 7, Bobcat L08-96; Lane 8, Bobcat L17-96; Lane 9, Bobcat DS08; Lane 10, Bobcat L10-97; Lane 11, Bobcat L11-97; Lane 12, Bobcat SC443; lane 13, Bobcat DS507; Lane 14, B . henselae Type I; Lane 15, B . henselae Type II; Lane 16, B . clarridgeiae ; Lane 17, B . koehlerae ; Lane18, B . bovis (“weissii” isolate).

    Journal: PLoS ONE

    Article Title: Isolation of Bartonella henselae and Two New Bartonella Subspecies, Bartonellakoehlerae Subspecies boulouisii subsp. nov. and Bartonella koehlerae Subspecies bothieri subsp. nov. from Free-Ranging Californian Mountain Lions and Bobcats

    doi: 10.1371/journal.pone.0148299

    Figure Lengend Snippet: PCR-RFLP of a gltA gene fragment using the restriction endonuclease Aci I. Lanes 1, 4 and 19 show 100 BP Ladder; Lane 2, Mt Lion L27-96; Lane 3, Mt Lion L42-94; Lane 5, Mt Lion L-39-97; Lane 6, Mt Lion FM98061; Lane 7, Bobcat L08-96; Lane 8, Bobcat L17-96; Lane 9, Bobcat DS08; Lane 10, Bobcat L10-97; Lane 11, Bobcat L11-97; Lane 12, Bobcat SC443; lane 13, Bobcat DS507; Lane 14, B . henselae Type I; Lane 15, B . henselae Type II; Lane 16, B . clarridgeiae ; Lane 17, B . koehlerae ; Lane18, B . bovis (“weissii” isolate).

    Article Snippet: The amplified product of the gltA gene was digested with Taq I (Promega, Madison, WI), Hha I (New England Biolabs, Beverly, M.A.), Mse I (New England Biolabs) and Aci I (New England Biolabs); the amplified product of the 16S rRNA gene was digested with Dde I (New England Biolabs); the amplified product of the ribC gene was digested with Taq I; and the amplified product of the 16S-23S ITS region was digested with Taq I and Hae III.

    Techniques: Polymerase Chain Reaction

    Fig. 1. Demethylation of tissue-specific gene promoter sequences in IVF (A and B) or cloned (C and D) bovine embryos. ( A and C ) The epidermal cytokeratin gene sequence (166 bp, four Aci I recognition sites). ( B and D ) The β-lactoglobulin gene sequence (110 bp, three Taq I sites). The number of embryos used in each lane was ∼200 for matured oocytes, ∼100 for one-cell eggs, ∼30 of four- to eight-cell embryos, ∼10 for morulae and about six for blastocysts. Both target regions consist of unique sequences with no interrupting repeat such as transposons. Photographs, negative images. Arrows, position of intact PCR products. M, DNA size marker (bp); X, intact, undigested PCR products; O, enzyme-treated PCR products; donor, fetal bovine fibroblasts; 4/8c, four- to eight-cell embryos; mor, morulae; bl, blastocysts.

    Journal: The EMBO Journal

    Article Title: Limited demethylation leaves mosaic-type methylation states in cloned bovine pre-implantation embryos

    doi: 10.1093/emboj/21.5.1092

    Figure Lengend Snippet: Fig. 1. Demethylation of tissue-specific gene promoter sequences in IVF (A and B) or cloned (C and D) bovine embryos. ( A and C ) The epidermal cytokeratin gene sequence (166 bp, four Aci I recognition sites). ( B and D ) The β-lactoglobulin gene sequence (110 bp, three Taq I sites). The number of embryos used in each lane was ∼200 for matured oocytes, ∼100 for one-cell eggs, ∼30 of four- to eight-cell embryos, ∼10 for morulae and about six for blastocysts. Both target regions consist of unique sequences with no interrupting repeat such as transposons. Photographs, negative images. Arrows, position of intact PCR products. M, DNA size marker (bp); X, intact, undigested PCR products; O, enzyme-treated PCR products; donor, fetal bovine fibroblasts; 4/8c, four- to eight-cell embryos; mor, morulae; bl, blastocysts.

    Article Snippet: One hundred nanograms of DNA were digested with 20 U of Aci I restriction enzyme (New England Biolabs) overnight at 37°C, resolved on 4% metaphore agarose gel or 5–8% polyacrylamide gel.

    Techniques: Clone Assay, Sequencing, Polymerase Chain Reaction, Marker

    Fig. 4. Methylation levels versus ICM/TE values in individual NT blastocysts. ( A ) ICM/TE ratios. ICM/TE values are indicated above the bars. Individual NT blastocysts are arranged (1–19) according to their ICM/TE ratios. ( B ) Methylation status of the satellite I sequences (upper panel) and the cytokeratin gene promoter sequence (lower panel). Numbers indicate percentage digestion calculated from summed band intensity of digested fragments relative to that of the whole fragments. The position of non-specifically amplified PCR bands is indicated by a dotted line on the lower panel. The satellite and the cytokeratin gene sequences were amplified from bisulfite-treated genomic DNA and then digested by Aci I enzyme, which recognizes 5′-GCGG-3′. Methylation status of both sequences of donor fibroblasts (cell) is included. Total cell numbers of individual blastocysts are counted (cell no.).

    Journal: The EMBO Journal

    Article Title: Limited demethylation leaves mosaic-type methylation states in cloned bovine pre-implantation embryos

    doi: 10.1093/emboj/21.5.1092

    Figure Lengend Snippet: Fig. 4. Methylation levels versus ICM/TE values in individual NT blastocysts. ( A ) ICM/TE ratios. ICM/TE values are indicated above the bars. Individual NT blastocysts are arranged (1–19) according to their ICM/TE ratios. ( B ) Methylation status of the satellite I sequences (upper panel) and the cytokeratin gene promoter sequence (lower panel). Numbers indicate percentage digestion calculated from summed band intensity of digested fragments relative to that of the whole fragments. The position of non-specifically amplified PCR bands is indicated by a dotted line on the lower panel. The satellite and the cytokeratin gene sequences were amplified from bisulfite-treated genomic DNA and then digested by Aci I enzyme, which recognizes 5′-GCGG-3′. Methylation status of both sequences of donor fibroblasts (cell) is included. Total cell numbers of individual blastocysts are counted (cell no.).

    Article Snippet: One hundred nanograms of DNA were digested with 20 U of Aci I restriction enzyme (New England Biolabs) overnight at 37°C, resolved on 4% metaphore agarose gel or 5–8% polyacrylamide gel.

    Techniques: Methylation, Sequencing, Amplification, Polymerase Chain Reaction

    The Klf1 exon 2 CGI is unmethylated during development. A ) Schematic of the mouse Klf1 gene across exon 2 and the flanking introns shows salient restriction enzyme sites within the region and a 1.1-kbp cDNA probe used for Southern blot. B ) Genomic DNA samples from whole testis between P5 and P45 (lanes 1–7, left and center panels) and P45 spleen (lane 8). DNAs were purified and double-digested with Eco RI and Bam HI restriction enzymes to liberate the 1.85-kb genomic fragment containing the Klf1 exon 2 CGI. Thereafter, the DNA samples were further digested with Hpa II (left panel) or not digested (center panel) and then Southern blotted using the 1.1-kb probe shown in A . In addition, several samples were methylated using Hpa II -methyltransferase then digested with Hpa II (right panel) and Southern blotted. Because of the total number of samples in this experiment, three Southern blots were generated, and the relevant lanes were used to form composite panels. Data for the P5–P15 lanes (break in Southern blots) were obtained from separate Southern blots. Arrowheads in A , locations of Hpa II restriction sites; FL in B , full-length 1.85-kb Eco RI - Bam HI fragments that are visible in the center and right panels; Spl, spleen.

    Journal: Biology of Reproduction

    Article Title: Tissue-Restricted Transcription from a Conserved Intragenic CpG Island in the Klf1 Gene in Mice 1

    doi: 10.1095/biolreprod.112.099879

    Figure Lengend Snippet: The Klf1 exon 2 CGI is unmethylated during development. A ) Schematic of the mouse Klf1 gene across exon 2 and the flanking introns shows salient restriction enzyme sites within the region and a 1.1-kbp cDNA probe used for Southern blot. B ) Genomic DNA samples from whole testis between P5 and P45 (lanes 1–7, left and center panels) and P45 spleen (lane 8). DNAs were purified and double-digested with Eco RI and Bam HI restriction enzymes to liberate the 1.85-kb genomic fragment containing the Klf1 exon 2 CGI. Thereafter, the DNA samples were further digested with Hpa II (left panel) or not digested (center panel) and then Southern blotted using the 1.1-kb probe shown in A . In addition, several samples were methylated using Hpa II -methyltransferase then digested with Hpa II (right panel) and Southern blotted. Because of the total number of samples in this experiment, three Southern blots were generated, and the relevant lanes were used to form composite panels. Data for the P5–P15 lanes (break in Southern blots) were obtained from separate Southern blots. Arrowheads in A , locations of Hpa II restriction sites; FL in B , full-length 1.85-kb Eco RI - Bam HI fragments that are visible in the center and right panels; Spl, spleen.

    Article Snippet: Genomic DNAs purified from testes were restriction digested with Eco RI and Bam HI (New England Biolabs) at 37°C for 4–6 h. DNA samples were then either restriction digested with Hpa II (New England Biolabs) for 4–6 h or methylated in vitro using Hpa II methyltransferase (New England Biolabs) and digested with Hpa II .

    Techniques: Southern Blot, Purification, Methylation, Generated

    Effect of MnCl 2 on the 3′ to 5′ exonuclease activities of UL30 and T4 DNA polymerases. ( A ) Autoradiogram of the reaction products with UL30 DNA polymerase. Reactions were performed as described in Materials and Methods with 20 nM platinated substrates D or E and 20 nM UL30 and either 10 mM MgCl 2 or 4 mM MnCl 2 for the times indicated. Lanes 1–10, substrate D. Lanes 11–20, substrate E. ( B ) Autoradiogram of the reaction products with T4 and UL30 DNA polymerases. Reactions were performed as described in Materials and Methods with 20 nM platinated substrate D and 20 nM UL30 or T4 polymerase and either 10 mM MgCl 2 or 4 mM MnCl 2 for the times indicated.

    Journal: Nucleic Acids Research

    Article Title: Effect of manganese on in vitro replication of damaged DNA catalyzed by the herpes simplex virus type-1 DNA polymerase

    doi:

    Figure Lengend Snippet: Effect of MnCl 2 on the 3′ to 5′ exonuclease activities of UL30 and T4 DNA polymerases. ( A ) Autoradiogram of the reaction products with UL30 DNA polymerase. Reactions were performed as described in Materials and Methods with 20 nM platinated substrates D or E and 20 nM UL30 and either 10 mM MgCl 2 or 4 mM MnCl 2 for the times indicated. Lanes 1–10, substrate D. Lanes 11–20, substrate E. ( B ) Autoradiogram of the reaction products with T4 and UL30 DNA polymerases. Reactions were performed as described in Materials and Methods with 20 nM platinated substrate D and 20 nM UL30 or T4 polymerase and either 10 mM MgCl 2 or 4 mM MnCl 2 for the times indicated.

    Article Snippet: T4 DNA polymerase, T4 polynucleotide kinase and Aci I restriction endonuclease were from New England Biolabs.

    Techniques:

    Nla III RLFP analysis of PCR-amplified crmB fragments from DNA of CPV isolates from human and animals. Nla III digest fragments after PCR amplification of OPV85 (human) (lane 1), OPV88/L (cat) (lane 2), OPV88/H (cat) (lane 3), OPV89/1 (cat)-M5 (lane 4), OPV89/2 (cat) (lane 5), OPV89/3 (cat) (lane 6), OPV89/4 (cat)-M6 (lane 7), OPV89/5 (cat)-M7 (lane 8), OPV90/1 (cat)-M8 (lane 9), OPV90/2 (human) (lane 10), OPV90/4 (dog) (lane 11), OPV90/5 (cat)-M9 (lane 12), OPV91/1 (cat) (lane 13), OPV91/2 (human) (lane 14), OPV91/3 (cow) (lane 15), CATPOX3 (lane 16), CATPOX5 (lane 17), RAT Moscow (rat) (lane 18), EP-1 (elephant) (lane 19), EP-2 (elephant)-M1 (lane 20), EP-3 (elephant) (lane 21), EP-4 (elephant) (lane 22), EP-5 (elephant) (lane 23), CPV BRT-Atlanta (lane 24), and CPV BRT-Munich (lane 25) are shown.

    Journal: Journal of Clinical Microbiology

    Article Title: Detection and Differentiation of Old World Orthopoxviruses: Restriction Fragment Length Polymorphism of the crmB Gene Region

    doi: 10.1128/JCM.39.1.94-100.2001

    Figure Lengend Snippet: Nla III RLFP analysis of PCR-amplified crmB fragments from DNA of CPV isolates from human and animals. Nla III digest fragments after PCR amplification of OPV85 (human) (lane 1), OPV88/L (cat) (lane 2), OPV88/H (cat) (lane 3), OPV89/1 (cat)-M5 (lane 4), OPV89/2 (cat) (lane 5), OPV89/3 (cat) (lane 6), OPV89/4 (cat)-M6 (lane 7), OPV89/5 (cat)-M7 (lane 8), OPV90/1 (cat)-M8 (lane 9), OPV90/2 (human) (lane 10), OPV90/4 (dog) (lane 11), OPV90/5 (cat)-M9 (lane 12), OPV91/1 (cat) (lane 13), OPV91/2 (human) (lane 14), OPV91/3 (cow) (lane 15), CATPOX3 (lane 16), CATPOX5 (lane 17), RAT Moscow (rat) (lane 18), EP-1 (elephant) (lane 19), EP-2 (elephant)-M1 (lane 20), EP-3 (elephant) (lane 21), EP-4 (elephant) (lane 22), EP-5 (elephant) (lane 23), CPV BRT-Atlanta (lane 24), and CPV BRT-Munich (lane 25) are shown.

    Article Snippet: Restriction reactions were done at 37°C with 50μl of the PCR product adjusted to recommended endonuclease buffer and 10 U of Nla III, Aci I, Nci I, or Sau 3A (New England Biolabs, Inc., Beverly, Mass.).

    Techniques: Polymerase Chain Reaction, Amplification

    Comparative Nla III RLFP analysis of PCR-amplified crmB fragments from intact genomes of OPV isolates. Nla III fragment sizes noted in parentheses were determined by direct sequence analysis of the crmB ) and agreed with sizes of fragments calculated by comparison to the 100-bp DNA ladder size marker (M). Lanes: 1, VAR major Bangladesh-1975; 2, VAR minor alastrim Brazil-Garcia-1966; 3, human MPV Congo-8; 4, human MPV 71-0082; 5, CML Somalia-1978; 6, CML (CP-5) Dubai-M4; 7, CPV Brighton; 8, CPV strain M1; 9, CPV strain M5; 10, CPV strain M6; 11, CPV strain M7; 12, CPV strain M8; 13, CPV strain M9; 14, CPV strain 58; 15, VAC Lister; 16, VAC Columbia; 17, BUF India-81-1985; 18, BUF India-3906; 19, RPV Utrecht; 20, TPV Dahomey-1971.

    Journal: Journal of Clinical Microbiology

    Article Title: Detection and Differentiation of Old World Orthopoxviruses: Restriction Fragment Length Polymorphism of the crmB Gene Region

    doi: 10.1128/JCM.39.1.94-100.2001

    Figure Lengend Snippet: Comparative Nla III RLFP analysis of PCR-amplified crmB fragments from intact genomes of OPV isolates. Nla III fragment sizes noted in parentheses were determined by direct sequence analysis of the crmB ) and agreed with sizes of fragments calculated by comparison to the 100-bp DNA ladder size marker (M). Lanes: 1, VAR major Bangladesh-1975; 2, VAR minor alastrim Brazil-Garcia-1966; 3, human MPV Congo-8; 4, human MPV 71-0082; 5, CML Somalia-1978; 6, CML (CP-5) Dubai-M4; 7, CPV Brighton; 8, CPV strain M1; 9, CPV strain M5; 10, CPV strain M6; 11, CPV strain M7; 12, CPV strain M8; 13, CPV strain M9; 14, CPV strain 58; 15, VAC Lister; 16, VAC Columbia; 17, BUF India-81-1985; 18, BUF India-3906; 19, RPV Utrecht; 20, TPV Dahomey-1971.

    Article Snippet: Restriction reactions were done at 37°C with 50μl of the PCR product adjusted to recommended endonuclease buffer and 10 U of Nla III, Aci I, Nci I, or Sau 3A (New England Biolabs, Inc., Beverly, Mass.).

    Techniques: Polymerase Chain Reaction, Amplification, Sequencing, Marker

    Nla III RLFP analysis of PCR-amplified crmB fragments from DNA of VAC variants. Nla III digest fragments of the PCR products of VAC COL (lane 1), VAC V (lane 2), VAC LIST (lane 3), VAC VNV (lane 4), VAC VCX (lane 5), VAC Wyeth (lane 6), VAC CV1-78 (lane 7), BUF 81 (lane 8), BUF 3906 (lane 9), RPV UTR (lane 10), Levaditi (lane 11), VAC M1 (lane 12), VAC COP wt (lane 13), VAC COP hr (lane 14), RPV Utrecht (lane 15), VAC Hagen (lane 16), VAC CVA (lane 17), VAC WR (lane 18), VAC Elstree (lane 19), and BUF BP-1 (lane 20) are shown.

    Journal: Journal of Clinical Microbiology

    Article Title: Detection and Differentiation of Old World Orthopoxviruses: Restriction Fragment Length Polymorphism of the crmB Gene Region

    doi: 10.1128/JCM.39.1.94-100.2001

    Figure Lengend Snippet: Nla III RLFP analysis of PCR-amplified crmB fragments from DNA of VAC variants. Nla III digest fragments of the PCR products of VAC COL (lane 1), VAC V (lane 2), VAC LIST (lane 3), VAC VNV (lane 4), VAC VCX (lane 5), VAC Wyeth (lane 6), VAC CV1-78 (lane 7), BUF 81 (lane 8), BUF 3906 (lane 9), RPV UTR (lane 10), Levaditi (lane 11), VAC M1 (lane 12), VAC COP wt (lane 13), VAC COP hr (lane 14), RPV Utrecht (lane 15), VAC Hagen (lane 16), VAC CVA (lane 17), VAC WR (lane 18), VAC Elstree (lane 19), and BUF BP-1 (lane 20) are shown.

    Article Snippet: Restriction reactions were done at 37°C with 50μl of the PCR product adjusted to recommended endonuclease buffer and 10 U of Nla III, Aci I, Nci I, or Sau 3A (New England Biolabs, Inc., Beverly, Mass.).

    Techniques: Polymerase Chain Reaction, Amplification

    RLFP analysis of PCR-amplified crmB fragments from genomes of CML and TPV isolates. Nla III digest fragments of the PCR products of CML Mauretania (lane 1), CML Somalia-1978 (lane 2), CML Niger (lane 3), CML (CP-1) Iran-M2 (lane 4), CML-Saudi-M3 (lane 5), and TPV Dahomey-1971 (lane 6) are shown in the left panel. Nci I digest fragments of the PCR products of TPV Dahomey-1971 (lane 7), CML Somalia-1978 (lane 8), CML Niger (lane 9), CML Mauretania (lane 10), and CML Iran-M2 (lane 11) are shown in the right panel. Size markers are provided by a 100-bp DNA ladder (lane M).

    Journal: Journal of Clinical Microbiology

    Article Title: Detection and Differentiation of Old World Orthopoxviruses: Restriction Fragment Length Polymorphism of the crmB Gene Region

    doi: 10.1128/JCM.39.1.94-100.2001

    Figure Lengend Snippet: RLFP analysis of PCR-amplified crmB fragments from genomes of CML and TPV isolates. Nla III digest fragments of the PCR products of CML Mauretania (lane 1), CML Somalia-1978 (lane 2), CML Niger (lane 3), CML (CP-1) Iran-M2 (lane 4), CML-Saudi-M3 (lane 5), and TPV Dahomey-1971 (lane 6) are shown in the left panel. Nci I digest fragments of the PCR products of TPV Dahomey-1971 (lane 7), CML Somalia-1978 (lane 8), CML Niger (lane 9), CML Mauretania (lane 10), and CML Iran-M2 (lane 11) are shown in the right panel. Size markers are provided by a 100-bp DNA ladder (lane M).

    Article Snippet: Restriction reactions were done at 37°C with 50μl of the PCR product adjusted to recommended endonuclease buffer and 10 U of Nla III, Aci I, Nci I, or Sau 3A (New England Biolabs, Inc., Beverly, Mass.).

    Techniques: Polymerase Chain Reaction, Amplification

    Agarose-gel analysis of tagmentation products of phage-λ DNA analyzed on ( A ) high-resolution and ( C ) low-resolution (i.e. mini) agarose gels. Size-distribution fits for the high-resolution gel ( B ) and mini gel ( D ) of the same tagmented λ-DNA sample subjected to increasing numbers of PCR-amplification cycles (lanes 3–5 in (A), lanes 2–4 in (C)): 8 cycles (top plots), 14 cycles (middle plots) and 20 cycles (bottom plots) in both (B) and (D). Vertical dashed lines (light red in (B), (D)) give the positions of maxima in the discrete molecular-weight ladder (blue ROI in (A), (C)).

    Journal: Nucleic Acids Research

    Article Title: Deconvolution of nucleic-acid length distributions: a gel electrophoresis analysis tool and applications

    doi: 10.1093/nar/gkz534

    Figure Lengend Snippet: Agarose-gel analysis of tagmentation products of phage-λ DNA analyzed on ( A ) high-resolution and ( C ) low-resolution (i.e. mini) agarose gels. Size-distribution fits for the high-resolution gel ( B ) and mini gel ( D ) of the same tagmented λ-DNA sample subjected to increasing numbers of PCR-amplification cycles (lanes 3–5 in (A), lanes 2–4 in (C)): 8 cycles (top plots), 14 cycles (middle plots) and 20 cycles (bottom plots) in both (B) and (D). Vertical dashed lines (light red in (B), (D)) give the positions of maxima in the discrete molecular-weight ladder (blue ROI in (A), (C)).

    Article Snippet: A reference mixture of λ-DNA fragments was prepared by digesting λ DNA to completion with 2 units of Aci I (New England Biolabs) per μg of λ DNA (New England Biolabs) for 2 h at 37°C.

    Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Amplification, Molecular Weight

    Different fingerprinting profiles obtained by digesting the tet (M) amplicons from 64 tet (M)-positive pneumococci with four endonucleases. Lane M, molecular size marker (100-bp ladder). Lane A, undigested tet (M) amplicon. Lanes 1a to 1e, different Aci I profiles ( Aci I 1 to Aci I 5 ). Lanes 2a and 2b, different Rsa I profiles ( Rsa I 1 and Rsa I 2 ). Lane 3, Mse I profile. Lane 4, Taq I profile.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Phenotypic and Molecular Characterization of Tetracycline- and Erythromycin-Resistant Strains of Streptococcus pneumoniae

    doi: 10.1128/AAC.47.7.2236-2241.2003

    Figure Lengend Snippet: Different fingerprinting profiles obtained by digesting the tet (M) amplicons from 64 tet (M)-positive pneumococci with four endonucleases. Lane M, molecular size marker (100-bp ladder). Lane A, undigested tet (M) amplicon. Lanes 1a to 1e, different Aci I profiles ( Aci I 1 to Aci I 5 ). Lanes 2a and 2b, different Rsa I profiles ( Rsa I 1 and Rsa I 2 ). Lane 3, Mse I profile. Lane 4, Taq I profile.

    Article Snippet: Briefly, a 10-μl aliquot of the PCR product obtained by using the primer pair described by Corso et al. ( ) from each tet (M)-positive isolate was digested with the following restriction endonucleases: Aci I, Mse I, Rsa I, and Taq I (New England Biolabs).

    Techniques: Marker, Amplification

    HRRA patterns of two tet (M)-positive pneumococci with restriction types A and B. Lane M, molecular size marker (100-bp ladder). Lane A, undigested tet (M) amplicon of the strain exhibiting restriction type A; lanes A1 to A4, restriction profiles yielded by endonucleases Aci I, Rsa I, Mse I, and Taq I, respectively. Lane B, undigested tet (M) amplicon of the strain exhibiting restriction type B; lanes B1 to B4, restriction profiles yielded by endonucleases Aci I, Rsa I, Mse I, and Taq I, respectively.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Phenotypic and Molecular Characterization of Tetracycline- and Erythromycin-Resistant Strains of Streptococcus pneumoniae

    doi: 10.1128/AAC.47.7.2236-2241.2003

    Figure Lengend Snippet: HRRA patterns of two tet (M)-positive pneumococci with restriction types A and B. Lane M, molecular size marker (100-bp ladder). Lane A, undigested tet (M) amplicon of the strain exhibiting restriction type A; lanes A1 to A4, restriction profiles yielded by endonucleases Aci I, Rsa I, Mse I, and Taq I, respectively. Lane B, undigested tet (M) amplicon of the strain exhibiting restriction type B; lanes B1 to B4, restriction profiles yielded by endonucleases Aci I, Rsa I, Mse I, and Taq I, respectively.

    Article Snippet: Briefly, a 10-μl aliquot of the PCR product obtained by using the primer pair described by Corso et al. ( ) from each tet (M)-positive isolate was digested with the following restriction endonucleases: Aci I, Mse I, Rsa I, and Taq I (New England Biolabs).

    Techniques: Marker, Amplification