Journal: Experimental & Molecular Medicine
Article Title: Loss of Pten synergizes with c-Met to promote hepatocellular carcinoma development via mTORC2 pathway
Figure Lengend Snippet: Molecular characterization of hepatocellular carcinomas developed in sgPten/c-Met mice. ( a ) Levels of activation of AKT/mTOR and Ras/MAPK pathways in wild-type (WT) and sgPten/c-Met mouse livers, as detected by western blot analysis. Representative blots are shown. GAPDH and β-actin were used as loading controls. ( b ) Immunohistochemical staining of WT and sgPten/c-Met mouse livers. Original magnifications: × 100 for Pten, p-AKT S473 , fatty acid synthase (FASN) and acetyl-CoA carboxylase (ACC) proteins; × 200 for Ki67. p, phosphorylated; t, total.
Article Snippet: Immunohistochemistry (IHC) was performed as previously described., The primary antibodies against c-Met (Abcam, Cambridge, MA, USA; 1:100), p-AKTS473 (Cell Signaling Technology, Danvers, MA, USA; 1:100), Pten (Cell Signaling Technology; 1:100), fatty acid synthase (FASN; Cell Signaling Technology; 1:150), acetyl-CoA carboxylase (ACC; Cell Signaling Technology; 1:100), p-ERK (Cell Signaling Technology; 1:100), and Ki67 (Thermo Fisher Scientific, Waltham, MA, USA; 1:150) were used in the present investigation.
Techniques: Mouse Assay, Activation Assay, Western Blot, Immunohistochemistry, Staining