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All (5)
Illumina
Standard Biotools
Oligos Etc
PrimerDesign
Spatial Transcriptomics
 

About 40 products / 426 snippets / 14 images

94 / 100
Bioz Stars
Access Array Barcode Library for Illumina Sequencers—384, Single Direction
Standard Biotools  
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Catalog#: 100-4876
Techniques
Polymerase Chain Reaction, Sequencing, Amplification, Purification (see more)
Article Snippets
"Each well received a separate primer pair with a unique 10-base barcode, obtained from the Access Array Barcode Library for Illumina (Item: 100-4876; Fluidigm, South San Francisco, CA, USA)."  (see more)
93 / 100
Bioz Stars
Access Array Barcode Library for Illumina Sequencers - 384
Standard Biotools  
star_border
Catalog#: 100-3771
Techniques
Polymerase Chain Reaction, Sequencing, Purification, DNA Sequencing (see more)
Article Snippets
".. Start Taq; 25 μl of pooled template after Agencourt XP bead cleanup; and 20 μl MiSeq (Fluidigm PN FLD-100-3771) or Ion Torrent (200 nM)."  (see more)
10+  more images
KEY RESOURCES TABLE
RECOMB
KNOCK-OUT
DNA-EX
AMP
SEQ
Polymerase-extension products generated during the first four cycles of the first stage of the DePCR are shown. After four linear cycles of copying, the first stage is terminated, samples are pooled and purified, and subsequently amplified <t>with</t> <t>Illumina</t> adapter primers. Primers used in this study are shown at the top of the figure, with different functional regions color coded. Red regions represent locus-specific portions of primers. Blue regions represent linker portions of primers. Yellow regions represent Illumina adapter sequences. Purple regions represent a variable, sample-specific <t>barcode.</t> Dotted lines represent nucleotide incorporation by DNA polymerase.
GENER
dPCR
PURI
AMP
FUNC-A
CS1, common sequence 1 linker sequence. CS2, common sequence 2 linker sequence. BC, <t>barcode.</t> F, Forward primer. R, Reverse primer, P5/P7, <t>Illumina</t> primers, PE1/PE2, Fluidigm Access Array Barcode Library Illumina adapters. In stage 1 (linear copying only), individual samples are cycled for four cycles with locus-specific primers and Fluidigm barcoded primers. Subsequently, all reactions are pooled and purified together, and then amplified with Illumina P5 and P7 primers in stage 2 (exponential amplification with primers targeting linker sequences). During stage 1, linear copying of templates leads to products which contain Illumina sequencing adapters, sample-specific barcodes, and the locus-specific region of interest. Only fragments with Illumina adapters and barcodes are exponentially amplified in stage 2. Locus-specific primer sequences can be modified as needed.
SEQ
PURI
AMP
MOD
×
90 / 100
Bioz Stars
Access Array barcodes
Standard Biotools  
star_border
Techniques
 
Article Snippets
"5ng of the sgRNA libraries were barcoded with Fluidigm Access Array barcodes using AccuPrime Supermix II (ThermoFisher) PCR mix (95°C for 5 m, 8 cycles of 95°C for .."  (see more)
94 / 100
Bioz Stars
450k array barcode
Illumina  
star_border
Techniques
 
Article Snippets
".. scanner (PC2, r =−0.46), time (days) between blood sampling and hybridization (PC3, r =−0.39), and Illumina 450k array barcode (PC4, r =0.18), because the first four PCs had an eigen value >1 ( ; .."  (see more)
91 / 100
Bioz Stars
Access Array barcode system
Standard Biotools  
star_border
Techniques
 
Article Snippets
".. according to our previous study : We barcoded the amplicons with the Access Array barcode system from Fluidigm in a two-step approach adapted from Illumina’s standard 16S profiling protocol. .."  (see more)
88 / 100
Bioz Stars
Access Array barcoding primers
Standard Biotools  
star_border
Techniques
 
Article Snippets
".. a 20 μl barcoding reaction that included 1x NEBNext Phusion Master (NEB) and 4 μl of the Fluidigm Access Array barcoding primers."  (see more)
86 / 100
Bioz Stars
Access Array barcode primers
Standard Biotools  
star_border
Techniques
 
Article Snippets
".. step was performed using 2 μl of the first-stage PCR product and Access Array barcode primers (Fluidigm, USA) for 8 cycles to generate the second-stage PCR products in 20 .."  (see more)
80 / 100
Bioz Stars
Access Array Barcode 384
Standard Biotools  
star_border
Techniques
 
Article Snippets
"PCRs for indexing contained 5 μL of purified PCR product, 2.5 μL of Fluidigm Access Array Barcode 384, and 1X KAPA HiFi HotStart ReadyMix (Roche Sequencing Solutions)."  (see more)
85 / 100
Bioz Stars
Access Array barcoding system
Standard Biotools  
star_border
Techniques
 
Article Snippets
"These amplifications were performed using the Fluidigm Access Array barcoding system (Fluidigm, South San Francisco, CA, USA)."  (see more)
91 / 100
Bioz Stars
Access Array Barcode Primers for Illumina
Illumina  
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Techniques
 
Article Snippets
".. FastStart High Fidelity Enzyme Blend (Roche), 1× Access Array Loading Reagent (Fluidigm), 400 nM Access Array Barcode Primers for Illumina, 30–70 ng/μL genomic DNA, 50 mM of each target- specific primer and .."  (see more)
80 / 100
Bioz Stars
Barcode array based spatial transcriptomics
Spatial Transcriptomics  
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Techniques
 
Article Snippets
"We demonstrated that this imaging-free strategy can be integrated with existing barcode array based spatial transcriptomics methods without perturbing spatial structures."  (see more)
IMG
DIFU-A
SEQ
CONJ-A
SD
85 / 100
Bioz Stars
384 Barcode Access Array primers
Standard Biotools  
star_border
Techniques
 
Article Snippets
"Ten nanograms of purified PCR product was amplified for a further 10 cycles using High Fidelity Master Mix (Roche) and 384 Barcode Access Array primers (Fluidigm)."  (see more)
LBLNG
INVITRO
INVIVO