absolute quantitation Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Thermo Fisher absolute quantitative pcr
    Absolute Quantitative Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/absolute quantitative pcr/product/Thermo Fisher
    Average 94 stars, based on 62 article reviews
    Price from $9.99 to $1999.99
    absolute quantitative pcr - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    99
    Millipore absolute quantification
    Absolute Quantification, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/absolute quantification/product/Millipore
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    absolute quantification - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    SCIEX absolute quantitation itraq
    Absolute Quantitation Itraq, supplied by SCIEX, used in various techniques. Bioz Stars score: 99/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/absolute quantitation itraq/product/SCIEX
    Average 99 stars, based on 69 article reviews
    Price from $9.99 to $1999.99
    absolute quantitation itraq - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    88
    Thermo Fisher absolute quantitation
    Absolute Quantitation, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/absolute quantitation/product/Thermo Fisher
    Average 88 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    absolute quantitation - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    99
    Thermo Fisher 7900ht fast real time pcr system absolute quantitation guide
    7900ht Fast Real Time Pcr System Absolute Quantitation Guide, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7900ht fast real time pcr system absolute quantitation guide/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    7900ht fast real time pcr system absolute quantitation guide - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    92
    ScienCell absolute human telomere length quantification qpcr assay kit
    The <t>telomere</t> length per each chromosome end (in kb) in hiPSCs and perinatal SCs was quantified by <t>qPCR.</t> All the cells were at 2–4 passages. The graphs show the mean ± SD of 5 independent experiments, * p
    Absolute Human Telomere Length Quantification Qpcr Assay Kit, supplied by ScienCell, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/absolute human telomere length quantification qpcr assay kit/product/ScienCell
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    absolute human telomere length quantification qpcr assay kit - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    88
    Thermo Fisher absolute quantitation itraq
    The Orc1BAH domain contributed to ORC's association with chromatin in yeast. ( A ) Outline of protocol used to compare immunopurified ORC from chromatin extracts prepared from ORC1 wild-type or orc1bah Δ mutant cells. See Supplemental Figure S1 for more detail. ( B ) The top panel on the left reports average <t>iTRAQ</t> ratios calculated for each ORC subunit except Orc1. (The Applied <t>Biosystems</t> algorithm could not determine the average iTRAQ ratio for the Orc1BAH domain and the remaining portion of Orc1, as it uses wild-type S. cerevisiae protein database as reference.) The top panel on the right reports average iTRAQ ratios calculated for each of the core histones and yeast histone H1. The average iTRAQ orc1bahΔ/ORC1 ratio for individual ORC subunits (average of all of the orc1bahΔ /ORC iTRAQ peptide ratios that comprised each polypeptide, determined by an Applied Biosystems algorithm) was close to 1.0, indicating that an anti-Orc1 IP recovered similar levels of ORC from wild-type ORC1 and orc1bahΔ mutant cells. In contrast, the average iTRAQ ratio for each of the core histones was ≤0.4, indicating that histones were recovered less efficiently from orc1bahΔ mutant compared with ORC1 cells. The bottom panel is a protein immunoblot of Orc1, Orc3, and Orc4 subunits from ORC1 wild-type and orc1bah Δ mutant cell extracts. A 600 cell equivalents of 0.25 (lanes 1 , 3 ) and 0.5 (lanes 2 , 4 ) were loaded. ( C ) Crude extracts from ORC1 wild-type or orc1bah Δ mutant cells (lanes 1–4 , total) were fractionated by centrifugation into soluble (lanes 5–8 , soluble) and chromatin-containing pellet (lanes 9–12 , pellet) fractions. A 600 ).
    Absolute Quantitation Itraq, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/absolute quantitation itraq/product/Thermo Fisher
    Average 88 stars, based on 42 article reviews
    Price from $9.99 to $1999.99
    absolute quantitation itraq - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    99
    Thermo Fisher absolute quantitative pcr sybr green mix
    The Orc1BAH domain contributed to ORC's association with chromatin in yeast. ( A ) Outline of protocol used to compare immunopurified ORC from chromatin extracts prepared from ORC1 wild-type or orc1bah Δ mutant cells. See Supplemental Figure S1 for more detail. ( B ) The top panel on the left reports average <t>iTRAQ</t> ratios calculated for each ORC subunit except Orc1. (The Applied <t>Biosystems</t> algorithm could not determine the average iTRAQ ratio for the Orc1BAH domain and the remaining portion of Orc1, as it uses wild-type S. cerevisiae protein database as reference.) The top panel on the right reports average iTRAQ ratios calculated for each of the core histones and yeast histone H1. The average iTRAQ orc1bahΔ/ORC1 ratio for individual ORC subunits (average of all of the orc1bahΔ /ORC iTRAQ peptide ratios that comprised each polypeptide, determined by an Applied Biosystems algorithm) was close to 1.0, indicating that an anti-Orc1 IP recovered similar levels of ORC from wild-type ORC1 and orc1bahΔ mutant cells. In contrast, the average iTRAQ ratio for each of the core histones was ≤0.4, indicating that histones were recovered less efficiently from orc1bahΔ mutant compared with ORC1 cells. The bottom panel is a protein immunoblot of Orc1, Orc3, and Orc4 subunits from ORC1 wild-type and orc1bah Δ mutant cell extracts. A 600 cell equivalents of 0.25 (lanes 1 , 3 ) and 0.5 (lanes 2 , 4 ) were loaded. ( C ) Crude extracts from ORC1 wild-type or orc1bah Δ mutant cells (lanes 1–4 , total) were fractionated by centrifugation into soluble (lanes 5–8 , soluble) and chromatin-containing pellet (lanes 9–12 , pellet) fractions. A 600 ).
    Absolute Quantitative Pcr Sybr Green Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/absolute quantitative pcr sybr green mix/product/Thermo Fisher
    Average 99 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    absolute quantitative pcr sybr green mix - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    92
    Thermo Fisher absolute quantification itraq reagent
    The Orc1BAH domain contributed to ORC's association with chromatin in yeast. ( A ) Outline of protocol used to compare immunopurified ORC from chromatin extracts prepared from ORC1 wild-type or orc1bah Δ mutant cells. See Supplemental Figure S1 for more detail. ( B ) The top panel on the left reports average <t>iTRAQ</t> ratios calculated for each ORC subunit except Orc1. (The Applied <t>Biosystems</t> algorithm could not determine the average iTRAQ ratio for the Orc1BAH domain and the remaining portion of Orc1, as it uses wild-type S. cerevisiae protein database as reference.) The top panel on the right reports average iTRAQ ratios calculated for each of the core histones and yeast histone H1. The average iTRAQ orc1bahΔ/ORC1 ratio for individual ORC subunits (average of all of the orc1bahΔ /ORC iTRAQ peptide ratios that comprised each polypeptide, determined by an Applied Biosystems algorithm) was close to 1.0, indicating that an anti-Orc1 IP recovered similar levels of ORC from wild-type ORC1 and orc1bahΔ mutant cells. In contrast, the average iTRAQ ratio for each of the core histones was ≤0.4, indicating that histones were recovered less efficiently from orc1bahΔ mutant compared with ORC1 cells. The bottom panel is a protein immunoblot of Orc1, Orc3, and Orc4 subunits from ORC1 wild-type and orc1bah Δ mutant cell extracts. A 600 cell equivalents of 0.25 (lanes 1 , 3 ) and 0.5 (lanes 2 , 4 ) were loaded. ( C ) Crude extracts from ORC1 wild-type or orc1bah Δ mutant cells (lanes 1–4 , total) were fractionated by centrifugation into soluble (lanes 5–8 , soluble) and chromatin-containing pellet (lanes 9–12 , pellet) fractions. A 600 ).
    Absolute Quantification Itraq Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/absolute quantification itraq reagent/product/Thermo Fisher
    Average 92 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    absolute quantification itraq reagent - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    99
    Thermo Fisher absolute fast probe based detection qpcr master mixes
    The Orc1BAH domain contributed to ORC's association with chromatin in yeast. ( A ) Outline of protocol used to compare immunopurified ORC from chromatin extracts prepared from ORC1 wild-type or orc1bah Δ mutant cells. See Supplemental Figure S1 for more detail. ( B ) The top panel on the left reports average <t>iTRAQ</t> ratios calculated for each ORC subunit except Orc1. (The Applied <t>Biosystems</t> algorithm could not determine the average iTRAQ ratio for the Orc1BAH domain and the remaining portion of Orc1, as it uses wild-type S. cerevisiae protein database as reference.) The top panel on the right reports average iTRAQ ratios calculated for each of the core histones and yeast histone H1. The average iTRAQ orc1bahΔ/ORC1 ratio for individual ORC subunits (average of all of the orc1bahΔ /ORC iTRAQ peptide ratios that comprised each polypeptide, determined by an Applied Biosystems algorithm) was close to 1.0, indicating that an anti-Orc1 IP recovered similar levels of ORC from wild-type ORC1 and orc1bahΔ mutant cells. In contrast, the average iTRAQ ratio for each of the core histones was ≤0.4, indicating that histones were recovered less efficiently from orc1bahΔ mutant compared with ORC1 cells. The bottom panel is a protein immunoblot of Orc1, Orc3, and Orc4 subunits from ORC1 wild-type and orc1bah Δ mutant cell extracts. A 600 cell equivalents of 0.25 (lanes 1 , 3 ) and 0.5 (lanes 2 , 4 ) were loaded. ( C ) Crude extracts from ORC1 wild-type or orc1bah Δ mutant cells (lanes 1–4 , total) were fractionated by centrifugation into soluble (lanes 5–8 , soluble) and chromatin-containing pellet (lanes 9–12 , pellet) fractions. A 600 ).
    Absolute Fast Probe Based Detection Qpcr Master Mixes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/absolute fast probe based detection qpcr master mixes/product/Thermo Fisher
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    absolute fast probe based detection qpcr master mixes - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher taqman gene expression absolute quantification assay
    The Orc1BAH domain contributed to ORC's association with chromatin in yeast. ( A ) Outline of protocol used to compare immunopurified ORC from chromatin extracts prepared from ORC1 wild-type or orc1bah Δ mutant cells. See Supplemental Figure S1 for more detail. ( B ) The top panel on the left reports average <t>iTRAQ</t> ratios calculated for each ORC subunit except Orc1. (The Applied <t>Biosystems</t> algorithm could not determine the average iTRAQ ratio for the Orc1BAH domain and the remaining portion of Orc1, as it uses wild-type S. cerevisiae protein database as reference.) The top panel on the right reports average iTRAQ ratios calculated for each of the core histones and yeast histone H1. The average iTRAQ orc1bahΔ/ORC1 ratio for individual ORC subunits (average of all of the orc1bahΔ /ORC iTRAQ peptide ratios that comprised each polypeptide, determined by an Applied Biosystems algorithm) was close to 1.0, indicating that an anti-Orc1 IP recovered similar levels of ORC from wild-type ORC1 and orc1bahΔ mutant cells. In contrast, the average iTRAQ ratio for each of the core histones was ≤0.4, indicating that histones were recovered less efficiently from orc1bahΔ mutant compared with ORC1 cells. The bottom panel is a protein immunoblot of Orc1, Orc3, and Orc4 subunits from ORC1 wild-type and orc1bah Δ mutant cell extracts. A 600 cell equivalents of 0.25 (lanes 1 , 3 ) and 0.5 (lanes 2 , 4 ) were loaded. ( C ) Crude extracts from ORC1 wild-type or orc1bah Δ mutant cells (lanes 1–4 , total) were fractionated by centrifugation into soluble (lanes 5–8 , soluble) and chromatin-containing pellet (lanes 9–12 , pellet) fractions. A 600 ).
    Taqman Gene Expression Absolute Quantification Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman gene expression absolute quantification assay/product/Thermo Fisher
    Average 99 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    taqman gene expression absolute quantification assay - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    85
    Thermo Fisher abi7500 absolute quantitation program
    The Orc1BAH domain contributed to ORC's association with chromatin in yeast. ( A ) Outline of protocol used to compare immunopurified ORC from chromatin extracts prepared from ORC1 wild-type or orc1bah Δ mutant cells. See Supplemental Figure S1 for more detail. ( B ) The top panel on the left reports average <t>iTRAQ</t> ratios calculated for each ORC subunit except Orc1. (The Applied <t>Biosystems</t> algorithm could not determine the average iTRAQ ratio for the Orc1BAH domain and the remaining portion of Orc1, as it uses wild-type S. cerevisiae protein database as reference.) The top panel on the right reports average iTRAQ ratios calculated for each of the core histones and yeast histone H1. The average iTRAQ orc1bahΔ/ORC1 ratio for individual ORC subunits (average of all of the orc1bahΔ /ORC iTRAQ peptide ratios that comprised each polypeptide, determined by an Applied Biosystems algorithm) was close to 1.0, indicating that an anti-Orc1 IP recovered similar levels of ORC from wild-type ORC1 and orc1bahΔ mutant cells. In contrast, the average iTRAQ ratio for each of the core histones was ≤0.4, indicating that histones were recovered less efficiently from orc1bahΔ mutant compared with ORC1 cells. The bottom panel is a protein immunoblot of Orc1, Orc3, and Orc4 subunits from ORC1 wild-type and orc1bah Δ mutant cell extracts. A 600 cell equivalents of 0.25 (lanes 1 , 3 ) and 0.5 (lanes 2 , 4 ) were loaded. ( C ) Crude extracts from ORC1 wild-type or orc1bah Δ mutant cells (lanes 1–4 , total) were fractionated by centrifugation into soluble (lanes 5–8 , soluble) and chromatin-containing pellet (lanes 9–12 , pellet) fractions. A 600 ).
    Abi7500 Absolute Quantitation Program, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi7500 absolute quantitation program/product/Thermo Fisher
    Average 85 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    abi7500 absolute quantitation program - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    95
    Thermo Fisher absolute quantitative pcr mix
    The Orc1BAH domain contributed to ORC's association with chromatin in yeast. ( A ) Outline of protocol used to compare immunopurified ORC from chromatin extracts prepared from ORC1 wild-type or orc1bah Δ mutant cells. See Supplemental Figure S1 for more detail. ( B ) The top panel on the left reports average <t>iTRAQ</t> ratios calculated for each ORC subunit except Orc1. (The Applied <t>Biosystems</t> algorithm could not determine the average iTRAQ ratio for the Orc1BAH domain and the remaining portion of Orc1, as it uses wild-type S. cerevisiae protein database as reference.) The top panel on the right reports average iTRAQ ratios calculated for each of the core histones and yeast histone H1. The average iTRAQ orc1bahΔ/ORC1 ratio for individual ORC subunits (average of all of the orc1bahΔ /ORC iTRAQ peptide ratios that comprised each polypeptide, determined by an Applied Biosystems algorithm) was close to 1.0, indicating that an anti-Orc1 IP recovered similar levels of ORC from wild-type ORC1 and orc1bahΔ mutant cells. In contrast, the average iTRAQ ratio for each of the core histones was ≤0.4, indicating that histones were recovered less efficiently from orc1bahΔ mutant compared with ORC1 cells. The bottom panel is a protein immunoblot of Orc1, Orc3, and Orc4 subunits from ORC1 wild-type and orc1bah Δ mutant cell extracts. A 600 cell equivalents of 0.25 (lanes 1 , 3 ) and 0.5 (lanes 2 , 4 ) were loaded. ( C ) Crude extracts from ORC1 wild-type or orc1bah Δ mutant cells (lanes 1–4 , total) were fractionated by centrifugation into soluble (lanes 5–8 , soluble) and chromatin-containing pellet (lanes 9–12 , pellet) fractions. A 600 ).
    Absolute Quantitative Pcr Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/absolute quantitative pcr mix/product/Thermo Fisher
    Average 95 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    absolute quantitative pcr mix - by Bioz Stars, 2020-09
    95/100 stars
      Buy from Supplier

    88
    Thermo Fisher absolute quantitation itraq reagents
    Discovery of somatostatin as a candidate interactor of oligomeric Aβ1-42. ( A ) Sequence alignment of preprocortistatin and preprosomatostatin. The signal sequence and the boundaries of the bioactive cortistatin and somatostatin peptides are indicated by horizontal bars. Identical residues are highlighted by black background shading, and peptide sequences observed by mass spectrometry are shown in colored fonts. ( B ) Example tandem MS spectrum supporting the identification of a peptide with the amino acid sequence ‘NFFWK’. Fragment masses attributed to B- and Y- ion series are shown in red and blue colors, respectively. ( C ) Expanded view of MS3 spectrum derived from ‘NFFWK’ parent spectrum in interactome study based on oAβ1-42-biotin bait and biotin only negative control (Experiment II). In this view, the relative intensities of signature ions reflect the relative abundances of the ‘NFFWK’ peptide in the six side-by-side generated affinity purification eluate fractions. ( D ) SST/CST in human frontal lobe extracts binds to oAβ1-42-biotin but not to N-terminal biotinylated or truncated Aβ baits. <t>iTRAQ</t> signature ion intensity distribution in experiment probing the relative ability of four different biotin baits to capture SST/CST from human brain extract. The exclusive presence of a high intensity 116 ion indicates that the ‘NFFWK’ fragment spectrum, which gave rise to this peak distribution, was dependent on SST/CST exclusively associating with oAβ1-42-biotin. ( E ) Preferential binding of SST to pre-aggregated oAβ1-42. <t>TMT</t> signature ion intensity distributions of four MS3 spectra assigned to preprosomatostatin based on oligomeric or monomeric Aβ1-42-biotin baits (Experiment III). PSMs derived from SST-14 (‘TFTSC’ and ‘NFFWK’ peptides) had in common signature ion intensity distributions characterized by high intensity even-numbered TMT fragments. In contrast, signature ion intensity distributions of preprosomatostatin-derived tryptic peptides outside of the SST-14 coding region were relatively evenly distributed. DOI: http://dx.doi.org/10.7554/eLife.28401.006
    Absolute Quantitation Itraq Reagents, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/absolute quantitation itraq reagents/product/Thermo Fisher
    Average 88 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    absolute quantitation itraq reagents - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    89
    Thermo Fisher absolute quantitation itraq reagent
    Discovery of somatostatin as a candidate interactor of oligomeric Aβ1-42. ( A ) Sequence alignment of preprocortistatin and preprosomatostatin. The signal sequence and the boundaries of the bioactive cortistatin and somatostatin peptides are indicated by horizontal bars. Identical residues are highlighted by black background shading, and peptide sequences observed by mass spectrometry are shown in colored fonts. ( B ) Example tandem MS spectrum supporting the identification of a peptide with the amino acid sequence ‘NFFWK’. Fragment masses attributed to B- and Y- ion series are shown in red and blue colors, respectively. ( C ) Expanded view of MS3 spectrum derived from ‘NFFWK’ parent spectrum in interactome study based on oAβ1-42-biotin bait and biotin only negative control (Experiment II). In this view, the relative intensities of signature ions reflect the relative abundances of the ‘NFFWK’ peptide in the six side-by-side generated affinity purification eluate fractions. ( D ) SST/CST in human frontal lobe extracts binds to oAβ1-42-biotin but not to N-terminal biotinylated or truncated Aβ baits. <t>iTRAQ</t> signature ion intensity distribution in experiment probing the relative ability of four different biotin baits to capture SST/CST from human brain extract. The exclusive presence of a high intensity 116 ion indicates that the ‘NFFWK’ fragment spectrum, which gave rise to this peak distribution, was dependent on SST/CST exclusively associating with oAβ1-42-biotin. ( E ) Preferential binding of SST to pre-aggregated oAβ1-42. <t>TMT</t> signature ion intensity distributions of four MS3 spectra assigned to preprosomatostatin based on oligomeric or monomeric Aβ1-42-biotin baits (Experiment III). PSMs derived from SST-14 (‘TFTSC’ and ‘NFFWK’ peptides) had in common signature ion intensity distributions characterized by high intensity even-numbered TMT fragments. In contrast, signature ion intensity distributions of preprosomatostatin-derived tryptic peptides outside of the SST-14 coding region were relatively evenly distributed. DOI: http://dx.doi.org/10.7554/eLife.28401.006
    Absolute Quantitation Itraq Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/absolute quantitation itraq reagent/product/Thermo Fisher
    Average 89 stars, based on 41 article reviews
    Price from $9.99 to $1999.99
    absolute quantitation itraq reagent - by Bioz Stars, 2020-09
    89/100 stars
      Buy from Supplier

    85
    Stratagene absolute quantitative real time
    Discovery of somatostatin as a candidate interactor of oligomeric Aβ1-42. ( A ) Sequence alignment of preprocortistatin and preprosomatostatin. The signal sequence and the boundaries of the bioactive cortistatin and somatostatin peptides are indicated by horizontal bars. Identical residues are highlighted by black background shading, and peptide sequences observed by mass spectrometry are shown in colored fonts. ( B ) Example tandem MS spectrum supporting the identification of a peptide with the amino acid sequence ‘NFFWK’. Fragment masses attributed to B- and Y- ion series are shown in red and blue colors, respectively. ( C ) Expanded view of MS3 spectrum derived from ‘NFFWK’ parent spectrum in interactome study based on oAβ1-42-biotin bait and biotin only negative control (Experiment II). In this view, the relative intensities of signature ions reflect the relative abundances of the ‘NFFWK’ peptide in the six side-by-side generated affinity purification eluate fractions. ( D ) SST/CST in human frontal lobe extracts binds to oAβ1-42-biotin but not to N-terminal biotinylated or truncated Aβ baits. <t>iTRAQ</t> signature ion intensity distribution in experiment probing the relative ability of four different biotin baits to capture SST/CST from human brain extract. The exclusive presence of a high intensity 116 ion indicates that the ‘NFFWK’ fragment spectrum, which gave rise to this peak distribution, was dependent on SST/CST exclusively associating with oAβ1-42-biotin. ( E ) Preferential binding of SST to pre-aggregated oAβ1-42. <t>TMT</t> signature ion intensity distributions of four MS3 spectra assigned to preprosomatostatin based on oligomeric or monomeric Aβ1-42-biotin baits (Experiment III). PSMs derived from SST-14 (‘TFTSC’ and ‘NFFWK’ peptides) had in common signature ion intensity distributions characterized by high intensity even-numbered TMT fragments. In contrast, signature ion intensity distributions of preprosomatostatin-derived tryptic peptides outside of the SST-14 coding region were relatively evenly distributed. DOI: http://dx.doi.org/10.7554/eLife.28401.006
    Absolute Quantitative Real Time, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/absolute quantitative real time/product/Stratagene
    Average 85 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    absolute quantitative real time - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    Image Search Results


    The telomere length per each chromosome end (in kb) in hiPSCs and perinatal SCs was quantified by qPCR. All the cells were at 2–4 passages. The graphs show the mean ± SD of 5 independent experiments, * p

    Journal: Cells

    Article Title: Epigenetic Features of Human Perinatal Stem Cells Redefine Their Stemness Potential

    doi: 10.3390/cells9051304

    Figure Lengend Snippet: The telomere length per each chromosome end (in kb) in hiPSCs and perinatal SCs was quantified by qPCR. All the cells were at 2–4 passages. The graphs show the mean ± SD of 5 independent experiments, * p

    Article Snippet: Absolute Telomere Length QuantificationDNA was extracted from hiPSCs, hFM-MSCs, hAECs, and hAFSCs using the blood and cell culture mini kit (QIAGEN, Hilden, Germany), quantified by Qubit 4 (Thermo Fisher Scientific, Waltham, MA, USA) and then analyzed by the absolute human telomere length quantification qPCR assay kit (ScienCell, Carlsbad, CA, USA) according to the manufacturer’s instructions.

    Techniques: Real-time Polymerase Chain Reaction

    The Orc1BAH domain contributed to ORC's association with chromatin in yeast. ( A ) Outline of protocol used to compare immunopurified ORC from chromatin extracts prepared from ORC1 wild-type or orc1bah Δ mutant cells. See Supplemental Figure S1 for more detail. ( B ) The top panel on the left reports average iTRAQ ratios calculated for each ORC subunit except Orc1. (The Applied Biosystems algorithm could not determine the average iTRAQ ratio for the Orc1BAH domain and the remaining portion of Orc1, as it uses wild-type S. cerevisiae protein database as reference.) The top panel on the right reports average iTRAQ ratios calculated for each of the core histones and yeast histone H1. The average iTRAQ orc1bahΔ/ORC1 ratio for individual ORC subunits (average of all of the orc1bahΔ /ORC iTRAQ peptide ratios that comprised each polypeptide, determined by an Applied Biosystems algorithm) was close to 1.0, indicating that an anti-Orc1 IP recovered similar levels of ORC from wild-type ORC1 and orc1bahΔ mutant cells. In contrast, the average iTRAQ ratio for each of the core histones was ≤0.4, indicating that histones were recovered less efficiently from orc1bahΔ mutant compared with ORC1 cells. The bottom panel is a protein immunoblot of Orc1, Orc3, and Orc4 subunits from ORC1 wild-type and orc1bah Δ mutant cell extracts. A 600 cell equivalents of 0.25 (lanes 1 , 3 ) and 0.5 (lanes 2 , 4 ) were loaded. ( C ) Crude extracts from ORC1 wild-type or orc1bah Δ mutant cells (lanes 1–4 , total) were fractionated by centrifugation into soluble (lanes 5–8 , soluble) and chromatin-containing pellet (lanes 9–12 , pellet) fractions. A 600 ).

    Journal: Genes & Development

    Article Title: The conserved bromo-adjacent homology domain of yeast Orc1 functions in the selection of DNA replication origins within chromatin

    doi: 10.1101/gad.1906410

    Figure Lengend Snippet: The Orc1BAH domain contributed to ORC's association with chromatin in yeast. ( A ) Outline of protocol used to compare immunopurified ORC from chromatin extracts prepared from ORC1 wild-type or orc1bah Δ mutant cells. See Supplemental Figure S1 for more detail. ( B ) The top panel on the left reports average iTRAQ ratios calculated for each ORC subunit except Orc1. (The Applied Biosystems algorithm could not determine the average iTRAQ ratio for the Orc1BAH domain and the remaining portion of Orc1, as it uses wild-type S. cerevisiae protein database as reference.) The top panel on the right reports average iTRAQ ratios calculated for each of the core histones and yeast histone H1. The average iTRAQ orc1bahΔ/ORC1 ratio for individual ORC subunits (average of all of the orc1bahΔ /ORC iTRAQ peptide ratios that comprised each polypeptide, determined by an Applied Biosystems algorithm) was close to 1.0, indicating that an anti-Orc1 IP recovered similar levels of ORC from wild-type ORC1 and orc1bahΔ mutant cells. In contrast, the average iTRAQ ratio for each of the core histones was ≤0.4, indicating that histones were recovered less efficiently from orc1bahΔ mutant compared with ORC1 cells. The bottom panel is a protein immunoblot of Orc1, Orc3, and Orc4 subunits from ORC1 wild-type and orc1bah Δ mutant cell extracts. A 600 cell equivalents of 0.25 (lanes 1 , 3 ) and 0.5 (lanes 2 , 4 ) were loaded. ( C ) Crude extracts from ORC1 wild-type or orc1bah Δ mutant cells (lanes 1–4 , total) were fractionated by centrifugation into soluble (lanes 5–8 , soluble) and chromatin-containing pellet (lanes 9–12 , pellet) fractions. A 600 ).

    Article Snippet: Relative levels of proteins recovered in the two different immunoprecipitates were determined using isobaric tags for relative and absolute quantitation (iTRAQ) (Applied Biosystems) ( ; ).

    Techniques: Mutagenesis, Western Blot, Centrifugation

    Discovery of somatostatin as a candidate interactor of oligomeric Aβ1-42. ( A ) Sequence alignment of preprocortistatin and preprosomatostatin. The signal sequence and the boundaries of the bioactive cortistatin and somatostatin peptides are indicated by horizontal bars. Identical residues are highlighted by black background shading, and peptide sequences observed by mass spectrometry are shown in colored fonts. ( B ) Example tandem MS spectrum supporting the identification of a peptide with the amino acid sequence ‘NFFWK’. Fragment masses attributed to B- and Y- ion series are shown in red and blue colors, respectively. ( C ) Expanded view of MS3 spectrum derived from ‘NFFWK’ parent spectrum in interactome study based on oAβ1-42-biotin bait and biotin only negative control (Experiment II). In this view, the relative intensities of signature ions reflect the relative abundances of the ‘NFFWK’ peptide in the six side-by-side generated affinity purification eluate fractions. ( D ) SST/CST in human frontal lobe extracts binds to oAβ1-42-biotin but not to N-terminal biotinylated or truncated Aβ baits. iTRAQ signature ion intensity distribution in experiment probing the relative ability of four different biotin baits to capture SST/CST from human brain extract. The exclusive presence of a high intensity 116 ion indicates that the ‘NFFWK’ fragment spectrum, which gave rise to this peak distribution, was dependent on SST/CST exclusively associating with oAβ1-42-biotin. ( E ) Preferential binding of SST to pre-aggregated oAβ1-42. TMT signature ion intensity distributions of four MS3 spectra assigned to preprosomatostatin based on oligomeric or monomeric Aβ1-42-biotin baits (Experiment III). PSMs derived from SST-14 (‘TFTSC’ and ‘NFFWK’ peptides) had in common signature ion intensity distributions characterized by high intensity even-numbered TMT fragments. In contrast, signature ion intensity distributions of preprosomatostatin-derived tryptic peptides outside of the SST-14 coding region were relatively evenly distributed. DOI: http://dx.doi.org/10.7554/eLife.28401.006

    Journal: eLife

    Article Title: Somatostatin binds to the human amyloid β peptide and favors the formation of distinct oligomers

    doi: 10.7554/eLife.28401

    Figure Lengend Snippet: Discovery of somatostatin as a candidate interactor of oligomeric Aβ1-42. ( A ) Sequence alignment of preprocortistatin and preprosomatostatin. The signal sequence and the boundaries of the bioactive cortistatin and somatostatin peptides are indicated by horizontal bars. Identical residues are highlighted by black background shading, and peptide sequences observed by mass spectrometry are shown in colored fonts. ( B ) Example tandem MS spectrum supporting the identification of a peptide with the amino acid sequence ‘NFFWK’. Fragment masses attributed to B- and Y- ion series are shown in red and blue colors, respectively. ( C ) Expanded view of MS3 spectrum derived from ‘NFFWK’ parent spectrum in interactome study based on oAβ1-42-biotin bait and biotin only negative control (Experiment II). In this view, the relative intensities of signature ions reflect the relative abundances of the ‘NFFWK’ peptide in the six side-by-side generated affinity purification eluate fractions. ( D ) SST/CST in human frontal lobe extracts binds to oAβ1-42-biotin but not to N-terminal biotinylated or truncated Aβ baits. iTRAQ signature ion intensity distribution in experiment probing the relative ability of four different biotin baits to capture SST/CST from human brain extract. The exclusive presence of a high intensity 116 ion indicates that the ‘NFFWK’ fragment spectrum, which gave rise to this peak distribution, was dependent on SST/CST exclusively associating with oAβ1-42-biotin. ( E ) Preferential binding of SST to pre-aggregated oAβ1-42. TMT signature ion intensity distributions of four MS3 spectra assigned to preprosomatostatin based on oligomeric or monomeric Aβ1-42-biotin baits (Experiment III). PSMs derived from SST-14 (‘TFTSC’ and ‘NFFWK’ peptides) had in common signature ion intensity distributions characterized by high intensity even-numbered TMT fragments. In contrast, signature ion intensity distributions of preprosomatostatin-derived tryptic peptides outside of the SST-14 coding region were relatively evenly distributed. DOI: http://dx.doi.org/10.7554/eLife.28401.006

    Article Snippet: The covalent modifications of primary amines with isobaric labels provided in the form of tandem mass tag (TMT) reagents (Thermo Fisher Scientific, Inc.) or isobaric tagging for relative and absolute quantitation (iTRAQ) reagents (Applied Biosystems, Foster City, CA, USA) followed instructions provided by the manufacturers.

    Techniques: Sequencing, Mass Spectrometry, Derivative Assay, Negative Control, Generated, Affinity Purification, Binding Assay