abi7500 real time pcr system Search Results


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  • 99
    Thermo Fisher abi7500 real time pcr systems
    <t>Prox1</t> is associated with IL-2 promoter Chromatin was extracted from ( A ) Jurkat cells and ( B ) naïve CD4 + T cells unstimulated or stimulated with anti-CD3/CD28 Dynabeads for 24 h, and precipitated with anti-Prox1 antibody or isotype IgG. The DNA sequence containing minimal IL-2 promoter (–256 to -46, product size: 211 bp) was analyzed by <t>PCR.</t> Data represent one of three separate experiments.
    Abi7500 Real Time Pcr Systems, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2085 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher abi7500 steponeplus real time pcr system
    <t>Prox1</t> is associated with IL-2 promoter Chromatin was extracted from ( A ) Jurkat cells and ( B ) naïve CD4 + T cells unstimulated or stimulated with anti-CD3/CD28 Dynabeads for 24 h, and precipitated with anti-Prox1 antibody or isotype IgG. The DNA sequence containing minimal IL-2 promoter (–256 to -46, product size: 211 bp) was analyzed by <t>PCR.</t> Data represent one of three separate experiments.
    Abi7500 Steponeplus Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher abi7500 fast real time pcr system machine
    <t>Prox1</t> is associated with IL-2 promoter Chromatin was extracted from ( A ) Jurkat cells and ( B ) naïve CD4 + T cells unstimulated or stimulated with anti-CD3/CD28 Dynabeads for 24 h, and precipitated with anti-Prox1 antibody or isotype IgG. The DNA sequence containing minimal IL-2 promoter (–256 to -46, product size: 211 bp) was analyzed by <t>PCR.</t> Data represent one of three separate experiments.
    Abi7500 Fast Real Time Pcr System Machine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi7500 fast real time pcr system machine/product/Thermo Fisher
    Average 99 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    abi7500 fast real time pcr system machine - by Bioz Stars, 2020-08
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    93
    Thermo Fisher abi7500 real time quantitative pcr system
    <t>Prox1</t> is associated with IL-2 promoter Chromatin was extracted from ( A ) Jurkat cells and ( B ) naïve CD4 + T cells unstimulated or stimulated with anti-CD3/CD28 Dynabeads for 24 h, and precipitated with anti-Prox1 antibody or isotype IgG. The DNA sequence containing minimal IL-2 promoter (–256 to -46, product size: 211 bp) was analyzed by <t>PCR.</t> Data represent one of three separate experiments.
    Abi7500 Real Time Quantitative Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
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    90
    Thermo Fisher abi7500 viia7 real time pcr detection system
    <t>Prox1</t> is associated with IL-2 promoter Chromatin was extracted from ( A ) Jurkat cells and ( B ) naïve CD4 + T cells unstimulated or stimulated with anti-CD3/CD28 Dynabeads for 24 h, and precipitated with anti-Prox1 antibody or isotype IgG. The DNA sequence containing minimal IL-2 promoter (–256 to -46, product size: 211 bp) was analyzed by <t>PCR.</t> Data represent one of three separate experiments.
    Abi7500 Viia7 Real Time Pcr Detection System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi7500 viia7 real time pcr detection system/product/Thermo Fisher
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    91
    Bio-Rad abi7500 real time pcr
    <t>Prox1</t> is associated with IL-2 promoter Chromatin was extracted from ( A ) Jurkat cells and ( B ) naïve CD4 + T cells unstimulated or stimulated with anti-CD3/CD28 Dynabeads for 24 h, and precipitated with anti-Prox1 antibody or isotype IgG. The DNA sequence containing minimal IL-2 promoter (–256 to -46, product size: 211 bp) was analyzed by <t>PCR.</t> Data represent one of three separate experiments.
    Abi7500 Real Time Pcr, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi7500 real time pcr/product/Bio-Rad
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    abi7500 real time pcr - by Bioz Stars, 2020-08
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    90
    Thermo Fisher abi7500 real time pcr machine
    <t>Prox1</t> is associated with IL-2 promoter Chromatin was extracted from ( A ) Jurkat cells and ( B ) naïve CD4 + T cells unstimulated or stimulated with anti-CD3/CD28 Dynabeads for 24 h, and precipitated with anti-Prox1 antibody or isotype IgG. The DNA sequence containing minimal IL-2 promoter (–256 to -46, product size: 211 bp) was analyzed by <t>PCR.</t> Data represent one of three separate experiments.
    Abi7500 Real Time Pcr Machine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 154 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi7500 real time pcr machine/product/Thermo Fisher
    Average 90 stars, based on 154 article reviews
    Price from $9.99 to $1999.99
    abi7500 real time pcr machine - by Bioz Stars, 2020-08
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    99
    Thermo Fisher 7500 real time pcr system
    <t>Prox1</t> is associated with IL-2 promoter Chromatin was extracted from ( A ) Jurkat cells and ( B ) naïve CD4 + T cells unstimulated or stimulated with anti-CD3/CD28 Dynabeads for 24 h, and precipitated with anti-Prox1 antibody or isotype IgG. The DNA sequence containing minimal IL-2 promoter (–256 to -46, product size: 211 bp) was analyzed by <t>PCR.</t> Data represent one of three separate experiments.
    7500 Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 54115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7500 real time pcr system/product/Thermo Fisher
    Average 99 stars, based on 54115 article reviews
    Price from $9.99 to $1999.99
    7500 real time pcr system - by Bioz Stars, 2020-08
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    91
    Promega abi7500 real time pcr amplifier
    <t>Prox1</t> is associated with IL-2 promoter Chromatin was extracted from ( A ) Jurkat cells and ( B ) naïve CD4 + T cells unstimulated or stimulated with anti-CD3/CD28 Dynabeads for 24 h, and precipitated with anti-Prox1 antibody or isotype IgG. The DNA sequence containing minimal IL-2 promoter (–256 to -46, product size: 211 bp) was analyzed by <t>PCR.</t> Data represent one of three separate experiments.
    Abi7500 Real Time Pcr Amplifier, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi7500 real time pcr amplifier/product/Promega
    Average 91 stars, based on 10 article reviews
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    abi7500 real time pcr amplifier - by Bioz Stars, 2020-08
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    85
    Thermo Fisher abi7500 real time pcr thermocycler
    <t>Prox1</t> is associated with IL-2 promoter Chromatin was extracted from ( A ) Jurkat cells and ( B ) naïve CD4 + T cells unstimulated or stimulated with anti-CD3/CD28 Dynabeads for 24 h, and precipitated with anti-Prox1 antibody or isotype IgG. The DNA sequence containing minimal IL-2 promoter (–256 to -46, product size: 211 bp) was analyzed by <t>PCR.</t> Data represent one of three separate experiments.
    Abi7500 Real Time Pcr Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Prox1 is associated with IL-2 promoter Chromatin was extracted from ( A ) Jurkat cells and ( B ) naïve CD4 + T cells unstimulated or stimulated with anti-CD3/CD28 Dynabeads for 24 h, and precipitated with anti-Prox1 antibody or isotype IgG. The DNA sequence containing minimal IL-2 promoter (–256 to -46, product size: 211 bp) was analyzed by PCR. Data represent one of three separate experiments.

    Journal: Oncotarget

    Article Title: Prox1 represses IL-2 gene expression by interacting with NFAT2

    doi: 10.18632/oncotarget.17278

    Figure Lengend Snippet: Prox1 is associated with IL-2 promoter Chromatin was extracted from ( A ) Jurkat cells and ( B ) naïve CD4 + T cells unstimulated or stimulated with anti-CD3/CD28 Dynabeads for 24 h, and precipitated with anti-Prox1 antibody or isotype IgG. The DNA sequence containing minimal IL-2 promoter (–256 to -46, product size: 211 bp) was analyzed by PCR. Data represent one of three separate experiments.

    Article Snippet: To check the mRNA levels of Prox1, IL-2 and GAPDH, real-time PCR was performed in triplicate with a real-time PCR system (ABI PRISM 7500; Applied Biosystems, Foster City, CA, USA) using a SYBR detection kit (Takara) according to the standard protocol.

    Techniques: Sequencing, Polymerase Chain Reaction

    Overexpression of Prox1 inhibits IL-2 expression ( A and B ) Lentiviral vector expressing Prox1 gene and GFP, or GFP alone, were generated. Jurkat cells were infected with Prox1 lentivirus or the control lentivirus. The GFP + Jurkat cells were sorted 48 h post-infection, and stimulated with anti-CD3/CD28 Dynabeads for indicated times. (A) Prox1 and (B) IL-2 mRNA levels were assessed by real-time PCR, while ( C ) IL-2 protein levels at 24 h were measured by ELISA. ( D ) Jurkat cells were transiently transfected with increasing amount of Prox1 plasmids (0.125, 0.25 and 0.5 μg/ml) or control vector (pcDNA3), and stimulated with anti-CD3/CD28 Dynabeads for 24 h. The IL-2 protein levels were measured by ELISA. The data from represent the mean ± SEM of three experiments, ** P

    Journal: Oncotarget

    Article Title: Prox1 represses IL-2 gene expression by interacting with NFAT2

    doi: 10.18632/oncotarget.17278

    Figure Lengend Snippet: Overexpression of Prox1 inhibits IL-2 expression ( A and B ) Lentiviral vector expressing Prox1 gene and GFP, or GFP alone, were generated. Jurkat cells were infected with Prox1 lentivirus or the control lentivirus. The GFP + Jurkat cells were sorted 48 h post-infection, and stimulated with anti-CD3/CD28 Dynabeads for indicated times. (A) Prox1 and (B) IL-2 mRNA levels were assessed by real-time PCR, while ( C ) IL-2 protein levels at 24 h were measured by ELISA. ( D ) Jurkat cells were transiently transfected with increasing amount of Prox1 plasmids (0.125, 0.25 and 0.5 μg/ml) or control vector (pcDNA3), and stimulated with anti-CD3/CD28 Dynabeads for 24 h. The IL-2 protein levels were measured by ELISA. The data from represent the mean ± SEM of three experiments, ** P

    Article Snippet: To check the mRNA levels of Prox1, IL-2 and GAPDH, real-time PCR was performed in triplicate with a real-time PCR system (ABI PRISM 7500; Applied Biosystems, Foster City, CA, USA) using a SYBR detection kit (Takara) according to the standard protocol.

    Techniques: Over Expression, Expressing, Plasmid Preparation, Generated, Infection, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Transfection

    Expression of Prox1 mRNA and protein in T cells ( A, B and C ) PBMCs, naïve CD4 + T cells and Jurkat cells were isolated, and stimulated with PHA and anti-CD3/CD28 Dynabeads for 24 h respectively. (A) Prox1 and (C) IL-2 mRNA levels were measured by RT-PCR, while (B) Prox1 protein levels were assessed by Western blot. For (A), (B) and (C), the data represent one of three independent experiments. ( D and E ) Naïve CD4 + T cells were stimulated with anti-CD3/CD28 Dynabeads for indicated times. (D) Prox1 and (E) IL-2 mRNA levels were measured by real-time PCR. Gene expression is normalized against the amount of GAPDH mRNA. The data from represent the mean ± SEM of three experiments.

    Journal: Oncotarget

    Article Title: Prox1 represses IL-2 gene expression by interacting with NFAT2

    doi: 10.18632/oncotarget.17278

    Figure Lengend Snippet: Expression of Prox1 mRNA and protein in T cells ( A, B and C ) PBMCs, naïve CD4 + T cells and Jurkat cells were isolated, and stimulated with PHA and anti-CD3/CD28 Dynabeads for 24 h respectively. (A) Prox1 and (C) IL-2 mRNA levels were measured by RT-PCR, while (B) Prox1 protein levels were assessed by Western blot. For (A), (B) and (C), the data represent one of three independent experiments. ( D and E ) Naïve CD4 + T cells were stimulated with anti-CD3/CD28 Dynabeads for indicated times. (D) Prox1 and (E) IL-2 mRNA levels were measured by real-time PCR. Gene expression is normalized against the amount of GAPDH mRNA. The data from represent the mean ± SEM of three experiments.

    Article Snippet: To check the mRNA levels of Prox1, IL-2 and GAPDH, real-time PCR was performed in triplicate with a real-time PCR system (ABI PRISM 7500; Applied Biosystems, Foster City, CA, USA) using a SYBR detection kit (Takara) according to the standard protocol.

    Techniques: Expressing, Isolation, Reverse Transcription Polymerase Chain Reaction, Western Blot, Real-time Polymerase Chain Reaction