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    Thermo Fisher abi prism 7900ht sequence detection system
    Transcriptional changes in the levels of selected xenobiotic metabolizing cytochrome P450s (CYPs) and apoptosis markers in PC12 cells exposed to MCP. (a) MCP-induced alterations in the mRNA expression of marker genes associated with metabolism of xenobiotics in PC12 cells. Quantitative Real Time PCR (RT-PCR q ) was performed in triplicate by TaqMan Probe using <t>ABI</t> <t>PRISM®</t> <t>7900HT</t> Sequence Detection System (Applied Biosystems, USA). Actin-β was used as internal control to normalize the data and MCP induced alterations in mRNA expression are expressed in relative quantity compared with respective unexposed control groups. (b) MCP induced alterations in the mRNA expression of marker genes associated with apoptosis in PC12 cells. Quantitative Real Time PCR (RT-PCR q ) was performed in triplicate by SYBR Green dye using ABI PRISM® 7900HT Sequence Detection System (Applied Biosystems, USA). Actin-β was used as internal control to normalize the data and MCP induced alterations in mRNA expression are expressed in relative quantity (RQ) compared with respective unexposed control groups. Reliability of Specific products was checked by melting curve analysis as well as running the product onto 2% agarose Gel.
    Abi Prism 7900ht Sequence Detection System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 24603 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Transcriptional changes in the levels of selected xenobiotic metabolizing cytochrome P450s (CYPs) and apoptosis markers in PC12 cells exposed to MCP. (a) MCP-induced alterations in the mRNA expression of marker genes associated with metabolism of xenobiotics in PC12 cells. Quantitative Real Time PCR (RT-PCR q ) was performed in triplicate by TaqMan Probe using ABI PRISM® 7900HT Sequence Detection System (Applied Biosystems, USA). Actin-β was used as internal control to normalize the data and MCP induced alterations in mRNA expression are expressed in relative quantity compared with respective unexposed control groups. (b) MCP induced alterations in the mRNA expression of marker genes associated with apoptosis in PC12 cells. Quantitative Real Time PCR (RT-PCR q ) was performed in triplicate by SYBR Green dye using ABI PRISM® 7900HT Sequence Detection System (Applied Biosystems, USA). Actin-β was used as internal control to normalize the data and MCP induced alterations in mRNA expression are expressed in relative quantity (RQ) compared with respective unexposed control groups. Reliability of Specific products was checked by melting curve analysis as well as running the product onto 2% agarose Gel.

    Journal: PLoS ONE

    Article Title: Monocrotophos Induced Apoptosis in PC12 Cells: Role of Xenobiotic Metabolizing Cytochrome P450s

    doi: 10.1371/journal.pone.0017757

    Figure Lengend Snippet: Transcriptional changes in the levels of selected xenobiotic metabolizing cytochrome P450s (CYPs) and apoptosis markers in PC12 cells exposed to MCP. (a) MCP-induced alterations in the mRNA expression of marker genes associated with metabolism of xenobiotics in PC12 cells. Quantitative Real Time PCR (RT-PCR q ) was performed in triplicate by TaqMan Probe using ABI PRISM® 7900HT Sequence Detection System (Applied Biosystems, USA). Actin-β was used as internal control to normalize the data and MCP induced alterations in mRNA expression are expressed in relative quantity compared with respective unexposed control groups. (b) MCP induced alterations in the mRNA expression of marker genes associated with apoptosis in PC12 cells. Quantitative Real Time PCR (RT-PCR q ) was performed in triplicate by SYBR Green dye using ABI PRISM® 7900HT Sequence Detection System (Applied Biosystems, USA). Actin-β was used as internal control to normalize the data and MCP induced alterations in mRNA expression are expressed in relative quantity (RQ) compared with respective unexposed control groups. Reliability of Specific products was checked by melting curve analysis as well as running the product onto 2% agarose Gel.

    Article Snippet: Quantitative Real Time PCR (RT-PCRq ) was performed in 96 well plate format using TaqMan primers and probes in ABI PRISM® 7900HT Sequence Detection System (Applied Biosystems, USA).

    Techniques: Expressing, Marker, Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Sequencing, SYBR Green Assay, Agarose Gel Electrophoresis

    Platform comparison. Allelic discrimination plots of the four GBA mutations. Water was used as the no template control. Fig. 1A: Validation on the ABI PRISM® 7900 HT Sequence Detection System. Fig. 1B: Validation on the Applied Biosystems ViiA™ 7 Real-Time PCR System. Fig. 1C: Blind validation on the ABI PRISM® 7900 HT Sequence Detection System.

    Journal: PLoS ONE

    Article Title: High-Throughput Carrier Screening Using TaqMan Allelic Discrimination

    doi: 10.1371/journal.pone.0059722

    Figure Lengend Snippet: Platform comparison. Allelic discrimination plots of the four GBA mutations. Water was used as the no template control. Fig. 1A: Validation on the ABI PRISM® 7900 HT Sequence Detection System. Fig. 1B: Validation on the Applied Biosystems ViiA™ 7 Real-Time PCR System. Fig. 1C: Blind validation on the ABI PRISM® 7900 HT Sequence Detection System.

    Article Snippet: Results The initial validation of the assays on a small scale yielded 100% genotyping accuracy. shows the successful genotyping of the 4 GBA mutations on both the ABI PRISM® 7900 HT Sequence Detection System and the Applied Biosystems ViiA™ 7 Real-Time PCR System (see and for other assay results).

    Techniques: Sequencing, Real-time Polymerase Chain Reaction

    Inflammatory factor profile in neuroblastoma. A. Total RNA was prepared from neuroblastoma tissues or para-tumor tissues using TRIzol reagent, reverse transcribed, and quantitative real-time PCR was carried out with specific primers on an ABI Prism 7900HT.

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: HMGB1-induced autophagy in Schwann cells promotes neuroblastoma proliferation

    doi:

    Figure Lengend Snippet: Inflammatory factor profile in neuroblastoma. A. Total RNA was prepared from neuroblastoma tissues or para-tumor tissues using TRIzol reagent, reverse transcribed, and quantitative real-time PCR was carried out with specific primers on an ABI Prism 7900HT.

    Article Snippet: Quantitative real-time PCR was carried out on an ABI Prism 7900HT (Applied Biosystems).

    Techniques: Real-time Polymerase Chain Reaction

    Effects of IRS-2 proteins on PDX1 gene transcription. A : Sixty-five islets remained untreated ( n.i. ) or were infected with adenoviral constructs harboring IRS-2 WT , IRS-2 5A , or empty vector for the indicated times. The level of PDX-1 mRNA was analyzed by real-time PCR. Data were normalized to the quantity of β-actin. B : 65 islets were treated with or without “0.2X Cytomix” for the indicated times, and PDX-1 mRNA level was analyzed by real-time PCR. Data were normalized to β-actin quantity. C : Sixty-five islets remained uninfected or were infected with adenoviral constructs. “0.2X Cytomix” was added for an additional 24 h. The level of PDX-1 mRNA was analyzed by real-time PCR. Data were analyzed by ABI PRISM SDS 7,000 software (Applied Biosystems). The data were normalized to the quantity of β-actin. Data are mean ± SEM of four experiments carried out in duplicate. P

    Journal: Diabetes

    Article Title: Elimination of Negative Feedback Control Mechanisms Along the Insulin Signaling Pathway Improves ?-Cell Function Under Stress

    doi: 10.2337/db09-0890

    Figure Lengend Snippet: Effects of IRS-2 proteins on PDX1 gene transcription. A : Sixty-five islets remained untreated ( n.i. ) or were infected with adenoviral constructs harboring IRS-2 WT , IRS-2 5A , or empty vector for the indicated times. The level of PDX-1 mRNA was analyzed by real-time PCR. Data were normalized to the quantity of β-actin. B : 65 islets were treated with or without “0.2X Cytomix” for the indicated times, and PDX-1 mRNA level was analyzed by real-time PCR. Data were normalized to β-actin quantity. C : Sixty-five islets remained uninfected or were infected with adenoviral constructs. “0.2X Cytomix” was added for an additional 24 h. The level of PDX-1 mRNA was analyzed by real-time PCR. Data were analyzed by ABI PRISM SDS 7,000 software (Applied Biosystems). The data were normalized to the quantity of β-actin. Data are mean ± SEM of four experiments carried out in duplicate. P

    Article Snippet: Quantitative detection of specific mRNA transcripts was carried out by real-time PCR using ABI-PRISM 7900HT instrument (Applied Biosystems, CA).

    Techniques: Infection, Construct, Plasmid Preparation, Real-time Polymerase Chain Reaction, Software

    SM induces the expression of proinflammatory cytokines/chemokines in lymph nodes and lungs. Animals were exposed to SM or vehicle, and total RNA was isolated from lymph node cells and lung tissues as described in Materials and Methods. The qPCR analysis was performed on the ABI PRISM 7900HT Real-Time PCR System using the One-Step RT-PCR Master Mix. The relative expression of each mRNA was calculated in lymph node cells (A) and lungs (B).

    Journal: International immunopharmacology

    Article Title: Sulfur Mustard Induces Immune Sensitization in Hairless Guinea Pigs

    doi: 10.1016/j.intimp.2009.10.015

    Figure Lengend Snippet: SM induces the expression of proinflammatory cytokines/chemokines in lymph nodes and lungs. Animals were exposed to SM or vehicle, and total RNA was isolated from lymph node cells and lung tissues as described in Materials and Methods. The qPCR analysis was performed on the ABI PRISM 7900HT Real-Time PCR System using the One-Step RT-PCR Master Mix. The relative expression of each mRNA was calculated in lymph node cells (A) and lungs (B).

    Article Snippet: The samples were resuspended in diethylpyrocarbonate (DEPC)-treated water (55°C for 10 min to dissolve RNA) and quantified spectrophotometrically. qPCR analysis was performed on the ABI PRISM 7900HT Real-Time PCR System using the One-Step RT-PCR Master Mix (Applied Biosystems, Foster City, CA).

    Techniques: Expressing, Isolation, Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction