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    Thermo Fisher abi 7500 fast realtime pcr system
    Transcription and expression of HCV core-IFN-α2a recombinant viruses. (A) Identification of pFBD-H1 and pFBD-H2. M: 1Kb Plus DNA ladder; pFBD-H1 and pFBD-H2 samples were digested by BamHI and EcoRI. (B) Identification of pFBD-H3 and pFBD-H4. M: 1Kb Plus DNA ladder; pFBD-H3 and pFBD-H4 samples were digested by BamHI and EcoRI. (C) <t>RT-PCR</t> results of HCV core gene in recombination viruses infect cells. Total RNA was isolated from Sf9 infected with vAcH1, vAcH2, vAcH3, or vAcH4. cDNA was synthesized with SuperScript First Strand Synthesis kit (Invitrogen) with 0.5 to 1.0 μg RNA according to the manufacturer’s instructions. Quantitative RT-PCR reactions were carried out using SYBR Green PCR master mix reagents on an <t>ABI</t> 7500 Fast Real-Time PCR System (Applied Biosystems). (D) Expression of HCV core-IFN-α2a fusion protein in recombinant virus infected cells. M: protein marker. Cells were harvested at 72 h post-infection (hpi) and lysed in SDS-PAGE loading buffer. Twenty micrograms of total protein was separated by SDS-PAGE and subjected to Western blot assay.
    Abi 7500 Fast Realtime Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2138 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi 7500 fast realtime pcr system/product/Thermo Fisher
    Average 99 stars, based on 2138 article reviews
    Price from $9.99 to $1999.99
    abi 7500 fast realtime pcr system - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher fast abi 7500 real time pcr system
    Transcription and expression of HCV core-IFN-α2a recombinant viruses. (A) Identification of pFBD-H1 and pFBD-H2. M: 1Kb Plus DNA ladder; pFBD-H1 and pFBD-H2 samples were digested by BamHI and EcoRI. (B) Identification of pFBD-H3 and pFBD-H4. M: 1Kb Plus DNA ladder; pFBD-H3 and pFBD-H4 samples were digested by BamHI and EcoRI. (C) <t>RT-PCR</t> results of HCV core gene in recombination viruses infect cells. Total RNA was isolated from Sf9 infected with vAcH1, vAcH2, vAcH3, or vAcH4. cDNA was synthesized with SuperScript First Strand Synthesis kit (Invitrogen) with 0.5 to 1.0 μg RNA according to the manufacturer’s instructions. Quantitative RT-PCR reactions were carried out using SYBR Green PCR master mix reagents on an <t>ABI</t> 7500 Fast Real-Time PCR System (Applied Biosystems). (D) Expression of HCV core-IFN-α2a fusion protein in recombinant virus infected cells. M: protein marker. Cells were harvested at 72 h post-infection (hpi) and lysed in SDS-PAGE loading buffer. Twenty micrograms of total protein was separated by SDS-PAGE and subjected to Western blot assay.
    Fast Abi 7500 Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fast abi 7500 real time pcr system/product/Thermo Fisher
    Average 99 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    fast abi 7500 real time pcr system - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

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    Transcription and expression of HCV core-IFN-α2a recombinant viruses. (A) Identification of pFBD-H1 and pFBD-H2. M: 1Kb Plus DNA ladder; pFBD-H1 and pFBD-H2 samples were digested by BamHI and EcoRI. (B) Identification of pFBD-H3 and pFBD-H4. M: 1Kb Plus DNA ladder; pFBD-H3 and pFBD-H4 samples were digested by BamHI and EcoRI. (C) RT-PCR results of HCV core gene in recombination viruses infect cells. Total RNA was isolated from Sf9 infected with vAcH1, vAcH2, vAcH3, or vAcH4. cDNA was synthesized with SuperScript First Strand Synthesis kit (Invitrogen) with 0.5 to 1.0 μg RNA according to the manufacturer’s instructions. Quantitative RT-PCR reactions were carried out using SYBR Green PCR master mix reagents on an ABI 7500 Fast Real-Time PCR System (Applied Biosystems). (D) Expression of HCV core-IFN-α2a fusion protein in recombinant virus infected cells. M: protein marker. Cells were harvested at 72 h post-infection (hpi) and lysed in SDS-PAGE loading buffer. Twenty micrograms of total protein was separated by SDS-PAGE and subjected to Western blot assay.

    Journal: Nanoscale Research Letters

    Article Title: Self-assembled HCV core virus-like particles targeted and inhibited tumor cell migration and invasion

    doi: 10.1186/1556-276X-8-401

    Figure Lengend Snippet: Transcription and expression of HCV core-IFN-α2a recombinant viruses. (A) Identification of pFBD-H1 and pFBD-H2. M: 1Kb Plus DNA ladder; pFBD-H1 and pFBD-H2 samples were digested by BamHI and EcoRI. (B) Identification of pFBD-H3 and pFBD-H4. M: 1Kb Plus DNA ladder; pFBD-H3 and pFBD-H4 samples were digested by BamHI and EcoRI. (C) RT-PCR results of HCV core gene in recombination viruses infect cells. Total RNA was isolated from Sf9 infected with vAcH1, vAcH2, vAcH3, or vAcH4. cDNA was synthesized with SuperScript First Strand Synthesis kit (Invitrogen) with 0.5 to 1.0 μg RNA according to the manufacturer’s instructions. Quantitative RT-PCR reactions were carried out using SYBR Green PCR master mix reagents on an ABI 7500 Fast Real-Time PCR System (Applied Biosystems). (D) Expression of HCV core-IFN-α2a fusion protein in recombinant virus infected cells. M: protein marker. Cells were harvested at 72 h post-infection (hpi) and lysed in SDS-PAGE loading buffer. Twenty micrograms of total protein was separated by SDS-PAGE and subjected to Western blot assay.

    Article Snippet: Quantitative RT-PCR reactions were carried out using SYBR Green PCR master mix reagents on an ABI 7500 Fast Real-Time PCR System (Applied Biosystems, Foster City, CA, USA).

    Techniques: Expressing, Recombinant, Reverse Transcription Polymerase Chain Reaction, Isolation, Infection, Synthesized, Quantitative RT-PCR, SYBR Green Assay, Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Marker, SDS Page, Western Blot

    Diosgenin up-regulated DDX3 expression. A. mRNA level of DDX3 was measured by q-PCR in diosgenin treated HepG2 and SMMC-7721 cells using power SYBR Green PCR Master Mix on an ABI Prism 7500 Fast Real-Time PCR system. The relative expression of DDX3 was calculated. GAPDH was used as an endogenous reference. B. The expression of DDX3, Cyclin D1, p21, Notch-1, E-cadherin, and β-catenin was detected by Western blotting analysis in diosgenin treated HepG2 and SMMC-7721 cells. *P

    Journal: American Journal of Translational Research

    Article Title: Diosgenin increased DDX3 expression in hepatocellular carcinoma

    doi:

    Figure Lengend Snippet: Diosgenin up-regulated DDX3 expression. A. mRNA level of DDX3 was measured by q-PCR in diosgenin treated HepG2 and SMMC-7721 cells using power SYBR Green PCR Master Mix on an ABI Prism 7500 Fast Real-Time PCR system. The relative expression of DDX3 was calculated. GAPDH was used as an endogenous reference. B. The expression of DDX3, Cyclin D1, p21, Notch-1, E-cadherin, and β-catenin was detected by Western blotting analysis in diosgenin treated HepG2 and SMMC-7721 cells. *P

    Article Snippet: For the quantification of DDX3 mRNAs, 2 μg total RNA was reverse-transcribed into cDNA using RevertAid First Strand cDNA Synthesis Kit. qPCR was performed using Power SYBR Green PCR Master Mix on an ABI Prism 7500 Fast Real-Time PCR system (Applied Biosystems, Foster City, CA, USA), the relative expression of DDX3 was calculated.

    Techniques: Expressing, Polymerase Chain Reaction, SYBR Green Assay, Real-time Polymerase Chain Reaction, Western Blot