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  • 94
    Thermo Fisher aari
    <t>pKIR1.1</t> construction. (A) For pKIR1.1, U6.26p::2 × <t>AarI:sgRNA</t> was inserted into the Sbf I site of pKIR1.0. (B) Flowchart of pKIR1.1-based vector construction. The upper sequence is an enlarged view of the boundary (yellow) between the U6.26 promoter (pale green) and the sgRNA scaffold (orange). This boundary contains an inverted repeat of Aar I recognition sites, and Aar I digestion generates four-base overhangs. A hybridized primer with overhangs can be inserted into this site as shown. G with a red triangle is the first base for RNA transcription from the U6.26 promoter. G + (N) 19 indicates the target sequence.
    Aari, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aari/product/Thermo Fisher
    Average 94 stars, based on 188 article reviews
    Price from $9.99 to $1999.99
    aari - by Bioz Stars, 2020-05
    94/100 stars
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    96
    Thermo Fisher b27
    CIC-3 immunoreactivity in a hippocampal neuronal apoptotic model (× 400). In the 3-morpholinosyndnomine (SIN-1; a nitric oxide donor) group, hippocampal neurons were cultured in <t>neurobasal</t> complete medium supplemented with SIN-1; in the SIN-1 + 4,4’-diisothiocyanostil bene-2,2’-disulfonic acid (DIDS; the chloride channel blocker) group, hippocampal neurons were cultured with medium containing SIN-1 and DIDS for 18 hours. Shown are images of the immunofluorescent staining of nuclei (blue, arrows indicate apoptotic neurons), of neurons with NeuN (red) and for chloride channels with CIC-3 (green). The apoptotic neurons and CIC-3 positive neurons in the SIN-1 group were significantly increased compared with the control group, and were significantly decreased in the SIN-1 + DIDS group compared with the SIN-1 group.
    B27, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 25776 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/b27/product/Thermo Fisher
    Average 96 stars, based on 25776 article reviews
    Price from $9.99 to $1999.99
    b27 - by Bioz Stars, 2020-05
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    Image Search Results


    pKIR1.1 construction. (A) For pKIR1.1, U6.26p::2 × AarI:sgRNA was inserted into the Sbf I site of pKIR1.0. (B) Flowchart of pKIR1.1-based vector construction. The upper sequence is an enlarged view of the boundary (yellow) between the U6.26 promoter (pale green) and the sgRNA scaffold (orange). This boundary contains an inverted repeat of Aar I recognition sites, and Aar I digestion generates four-base overhangs. A hybridized primer with overhangs can be inserted into this site as shown. G with a red triangle is the first base for RNA transcription from the U6.26 promoter. G + (N) 19 indicates the target sequence.

    Journal: Plant and Cell Physiology

    Article Title: pKAMA-ITACHI Vectors for Highly Efficient CRISPR/Cas9-Mediated Gene Knockout in Arabidopsis thaliana

    doi: 10.1093/pcp/pcw191

    Figure Lengend Snippet: pKIR1.1 construction. (A) For pKIR1.1, U6.26p::2 × AarI:sgRNA was inserted into the Sbf I site of pKIR1.0. (B) Flowchart of pKIR1.1-based vector construction. The upper sequence is an enlarged view of the boundary (yellow) between the U6.26 promoter (pale green) and the sgRNA scaffold (orange). This boundary contains an inverted repeat of Aar I recognition sites, and Aar I digestion generates four-base overhangs. A hybridized primer with overhangs can be inserted into this site as shown. G with a red triangle is the first base for RNA transcription from the U6.26 promoter. G + (N) 19 indicates the target sequence.

    Article Snippet: For pKIR1.1_PSD3, pKIR1.1_ADH1-2 and pKI1.1R_PDS3, pKIR1.1 or pKI1.1R was digested by Aar I (Thermo Fisher Scientific, #ER1581) with alkaline phosphatase rAPid (Roche, #04898133001).

    Techniques: Plasmid Preparation, Sequencing

    CIC-3 immunoreactivity in a hippocampal neuronal apoptotic model (× 400). In the 3-morpholinosyndnomine (SIN-1; a nitric oxide donor) group, hippocampal neurons were cultured in neurobasal complete medium supplemented with SIN-1; in the SIN-1 + 4,4’-diisothiocyanostil bene-2,2’-disulfonic acid (DIDS; the chloride channel blocker) group, hippocampal neurons were cultured with medium containing SIN-1 and DIDS for 18 hours. Shown are images of the immunofluorescent staining of nuclei (blue, arrows indicate apoptotic neurons), of neurons with NeuN (red) and for chloride channels with CIC-3 (green). The apoptotic neurons and CIC-3 positive neurons in the SIN-1 group were significantly increased compared with the control group, and were significantly decreased in the SIN-1 + DIDS group compared with the SIN-1 group.

    Journal: Neural Regeneration Research

    Article Title: ClC-3 chloride channel in hippocampal neuronal apoptosis

    doi: 10.3969/j.issn.1673-5374.2013.32.008

    Figure Lengend Snippet: CIC-3 immunoreactivity in a hippocampal neuronal apoptotic model (× 400). In the 3-morpholinosyndnomine (SIN-1; a nitric oxide donor) group, hippocampal neurons were cultured in neurobasal complete medium supplemented with SIN-1; in the SIN-1 + 4,4’-diisothiocyanostil bene-2,2’-disulfonic acid (DIDS; the chloride channel blocker) group, hippocampal neurons were cultured with medium containing SIN-1 and DIDS for 18 hours. Shown are images of the immunofluorescent staining of nuclei (blue, arrows indicate apoptotic neurons), of neurons with NeuN (red) and for chloride channels with CIC-3 (green). The apoptotic neurons and CIC-3 positive neurons in the SIN-1 group were significantly increased compared with the control group, and were significantly decreased in the SIN-1 + DIDS group compared with the SIN-1 group.

    Article Snippet: Afterwards, the culture medium was replenished with neurobasal medium (Gibco; containing B27).

    Techniques: Cell Culture, Staining

    Effect of 4,4’-diisothiocyanostilbene-2,2’-disulfonic acid (DIDS; the chloride channel blocker) on ClC-3 mRNA in a hippocampal neuronal apoptosis model. In the 3-morpholinosyndnomine (SIN-1; a nitric oxide donor) group, hippocampal neurons were cultured in neurobasal complete medium supplemented with SIN-1; in the SIN-1 + DIDS group, hippocampal neurons were cultured with medium containing SIN-1 and DIDS for 18 hours. Real-time PCR assay was used to detect ClC-3 chloride channel mRNA expression, with the GADPH level used as an internal reference. (A) Electrophoresis of the glyceraldehyde-3-phosphate dehydrogenase gene (GADPH) PCR amplification product (110 bp). (B) Electrophoresis of the ClC-3 gene PCR amplification product (116 bp). Lanes 1–3: Control group; lanes 4–6: SIN-1 group; lanes 7–9: SIN-1 + DIDS group; M: marker. (C) Quantification of ClC-3 mRNA expression. The data from three independent experiments are expressed as mean ± SD, n = 6, and analyzed using one-way analysis of variance followed by least significant difference test. a P

    Journal: Neural Regeneration Research

    Article Title: ClC-3 chloride channel in hippocampal neuronal apoptosis

    doi: 10.3969/j.issn.1673-5374.2013.32.008

    Figure Lengend Snippet: Effect of 4,4’-diisothiocyanostilbene-2,2’-disulfonic acid (DIDS; the chloride channel blocker) on ClC-3 mRNA in a hippocampal neuronal apoptosis model. In the 3-morpholinosyndnomine (SIN-1; a nitric oxide donor) group, hippocampal neurons were cultured in neurobasal complete medium supplemented with SIN-1; in the SIN-1 + DIDS group, hippocampal neurons were cultured with medium containing SIN-1 and DIDS for 18 hours. Real-time PCR assay was used to detect ClC-3 chloride channel mRNA expression, with the GADPH level used as an internal reference. (A) Electrophoresis of the glyceraldehyde-3-phosphate dehydrogenase gene (GADPH) PCR amplification product (110 bp). (B) Electrophoresis of the ClC-3 gene PCR amplification product (116 bp). Lanes 1–3: Control group; lanes 4–6: SIN-1 group; lanes 7–9: SIN-1 + DIDS group; M: marker. (C) Quantification of ClC-3 mRNA expression. The data from three independent experiments are expressed as mean ± SD, n = 6, and analyzed using one-way analysis of variance followed by least significant difference test. a P

    Article Snippet: Afterwards, the culture medium was replenished with neurobasal medium (Gibco; containing B27).

    Techniques: Cell Culture, Real-time Polymerase Chain Reaction, Expressing, Electrophoresis, Polymerase Chain Reaction, Amplification, Marker

    Effect of 4,4’-diisothiocyanostilbene-2,2’-disulfonic acid (DIDS; the chloride channel blocker) on cell viability in a hippocampal neuronal apoptotic model. In the 3-morpholinosyndnomine (SIN-1; a nitric oxide donor) group, hippocampal neurons were cultured in neurobasal complete medium supplemented with SIN-1; in the SIN-1 + DIDS group, hippocampal neurons were cultured with medium containing SIN-1 and DIDS for 18 hours. The neuronal survival rate was detected by the MTT assay. The wells without cultured neurons were used as the blank. The data from three independent experiment are expressed as mean ± SD, n = 6, and analyzed using one-way analysis of variance followed by least significant difference test. a P

    Journal: Neural Regeneration Research

    Article Title: ClC-3 chloride channel in hippocampal neuronal apoptosis

    doi: 10.3969/j.issn.1673-5374.2013.32.008

    Figure Lengend Snippet: Effect of 4,4’-diisothiocyanostilbene-2,2’-disulfonic acid (DIDS; the chloride channel blocker) on cell viability in a hippocampal neuronal apoptotic model. In the 3-morpholinosyndnomine (SIN-1; a nitric oxide donor) group, hippocampal neurons were cultured in neurobasal complete medium supplemented with SIN-1; in the SIN-1 + DIDS group, hippocampal neurons were cultured with medium containing SIN-1 and DIDS for 18 hours. The neuronal survival rate was detected by the MTT assay. The wells without cultured neurons were used as the blank. The data from three independent experiment are expressed as mean ± SD, n = 6, and analyzed using one-way analysis of variance followed by least significant difference test. a P

    Article Snippet: Afterwards, the culture medium was replenished with neurobasal medium (Gibco; containing B27).

    Techniques: Cell Culture, MTT Assay

    Effect of 4,4’-diisothiocyanostilbene-2,2’-disulfonic acid (DIDS; the chloride channel blocker) on caspase-3 protein expression in a hippocampal neuronal apoptosis model. In the 3-morpholinosyndnomine (SIN-1; a nitric oxide donor) group, hippocampal neurons were cultured in neurobasal complete medium supplemented with SIN-1; in the SIN-1 + DIDS group, hippocampal neurons were cultured with medium containing SIN-1 and DIDS for 18 hours. Western blot analysis was performed to detect the expression of activated caspase-3, with the β-actin level used as an internal control. (A) Protein electrophoretic band diagram. (B) Quantification of caspase-3 protein expression (gray value ratio of each band to the gray value of β-actin). The data from three independent experiments are expressed as mean ± SD, n = 6, and analyzed using one-way analysis of variance followed by least significant difference test. a P

    Journal: Neural Regeneration Research

    Article Title: ClC-3 chloride channel in hippocampal neuronal apoptosis

    doi: 10.3969/j.issn.1673-5374.2013.32.008

    Figure Lengend Snippet: Effect of 4,4’-diisothiocyanostilbene-2,2’-disulfonic acid (DIDS; the chloride channel blocker) on caspase-3 protein expression in a hippocampal neuronal apoptosis model. In the 3-morpholinosyndnomine (SIN-1; a nitric oxide donor) group, hippocampal neurons were cultured in neurobasal complete medium supplemented with SIN-1; in the SIN-1 + DIDS group, hippocampal neurons were cultured with medium containing SIN-1 and DIDS for 18 hours. Western blot analysis was performed to detect the expression of activated caspase-3, with the β-actin level used as an internal control. (A) Protein electrophoretic band diagram. (B) Quantification of caspase-3 protein expression (gray value ratio of each band to the gray value of β-actin). The data from three independent experiments are expressed as mean ± SD, n = 6, and analyzed using one-way analysis of variance followed by least significant difference test. a P

    Article Snippet: Afterwards, the culture medium was replenished with neurobasal medium (Gibco; containing B27).

    Techniques: Expressing, Cell Culture, Western Blot

    Effect of 4,4’-diisothiocyanostilbene-2,2’-disulfonic acid (DIDS; the chloride channel blocker) on ClC-3 chloride channel protein in a hippocampal neuronal apoptosis model. In the 3-morpholinosyndnomine (SIN-1; a nitric oxide donor) group, hippocampal neurons were cultured in neurobasal complete medium supplemented with SIN-1; in the SIN-1 + DIDS group, hippocampal neurons were cultured with medium containing SIN-1 and DIDS for 18 hours. Western blot analysis was performed to detect the expression of ClC-3 channels, and the β-actin level was used as an internal control. (A) Protein electrophoretic band diagram; (B) Quantification of ClC-3 protein expression (gray value ratio of each band to the gray value of β-actin). The data from three independent experiments are expressed as mean ± SD, n = 6, and analyzed using one-way analysis of variance followed by least significant difference test. a P

    Journal: Neural Regeneration Research

    Article Title: ClC-3 chloride channel in hippocampal neuronal apoptosis

    doi: 10.3969/j.issn.1673-5374.2013.32.008

    Figure Lengend Snippet: Effect of 4,4’-diisothiocyanostilbene-2,2’-disulfonic acid (DIDS; the chloride channel blocker) on ClC-3 chloride channel protein in a hippocampal neuronal apoptosis model. In the 3-morpholinosyndnomine (SIN-1; a nitric oxide donor) group, hippocampal neurons were cultured in neurobasal complete medium supplemented with SIN-1; in the SIN-1 + DIDS group, hippocampal neurons were cultured with medium containing SIN-1 and DIDS for 18 hours. Western blot analysis was performed to detect the expression of ClC-3 channels, and the β-actin level was used as an internal control. (A) Protein electrophoretic band diagram; (B) Quantification of ClC-3 protein expression (gray value ratio of each band to the gray value of β-actin). The data from three independent experiments are expressed as mean ± SD, n = 6, and analyzed using one-way analysis of variance followed by least significant difference test. a P

    Article Snippet: Afterwards, the culture medium was replenished with neurobasal medium (Gibco; containing B27).

    Techniques: Cell Culture, Western Blot, Expressing