a53t αsyn Search Results


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  • 99
    Thermo Fisher egfp αsyn a53t hela cells
    Intracellular effects of PAR on <t>αSyn.</t> (a) Cytotoxicity assay on SH-SY5Y neuroblastoma cells treated in triplicate with three different concentrations of PAR polymer for 48 h. (b) <t>Hela</t> cells transfected with <t>EGFP-αSyn-A53T</t> and treated with PAR (50 nM) for 48 h; representative ROIs from merge channel images showing αSyn A53T-EGFP aggregates (green) and increased superoxide levels i.e. higher red intensity, in the PAR treated vs. BioPORTER alone (vehicle control) cells. Images were captured using Zeiss Axio Widefield (20x/0.8) microscope. Experiments were performed in triplicate. (c) Representative Western blot and graph of γH2AX levels in PAR, PAR + 1 μM PDD00017273 (PARGi) and ADP-HDP treated SH-SY5Y-αSyn cells. Experiments were repeated independently three times (n =3). (d and e) Quantification data from a time course PLA on SH-SY5Y-αSyn cells treated with PAR, PAR + 1 μM PDD00017273 (PARGi) or ADP-HDP for 4 h (d) and 24 h (e). Bars represent means ± SEM. Two-way ANOVA followed by Tukey’s post hoc test (n =3). ****P
    Egfp αsyn A53t Hela Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egfp αsyn a53t hela cells/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    egfp αsyn a53t hela cells - by Bioz Stars, 2020-09
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    84
    The Jackson Laboratory animals a53t αsyn tg mice
    APOE genotypes differentially regulate <t>αSyn</t> aggregation and phosphorylation in <t>A53T</t> mice. (A-D) Total αSyn concentration was measured by ELISA in RAB, RAB+TX-100, RIPA, and SDS fractions from the brainstem of 12-month old A53T mice. A53T/EKO, n=10; A53T/E2, n=6; A53T/E3, n=9; A53T/E4, n=10. Closed symbols indicate asymptomatic mice; open symbols indicate symptomatic mice with endstage paralysis. Symbols shown in black in (D) indicate A53T/EKO and A53T/E3 samples below the limit of detection. (E-F) Phospho-αSyn concentration was measured by ELISA in RIPA and SDS fractions. Data expressed as mean ± SEM, one-way ANOVA with Tukey’s multiple comparisons test (A, B, E, F) or Kruskal-Wallis test with Dunn’s multiple comparisons test (C) . *p
    Animals A53t αsyn Tg Mice, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/animals a53t αsyn tg mice/product/The Jackson Laboratory
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    animals a53t αsyn tg mice - by Bioz Stars, 2020-09
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    84
    The Jackson Laboratory human a53t variant αsyn
    Intracellular effects of PAR on <t>αSyn.</t> (a) Cytotoxicity assay on SH-SY5Y neuroblastoma cells treated in triplicate with three different concentrations of PAR polymer for 48 h. (b) Hela cells transfected with <t>EGFP-αSyn-A53T</t> and treated with PAR (50 nM) for 48 h; representative ROIs from merge channel images showing αSyn A53T-EGFP aggregates (green) and increased superoxide levels i.e. higher red intensity, in the PAR treated vs. BioPORTER alone (vehicle control) cells. Images were captured using Zeiss Axio Widefield (20x/0.8) microscope. Experiments were performed in triplicate. (c) Representative Western blot and graph of γH2AX levels in PAR, PAR + 1 μM PDD00017273 (PARGi) and ADP-HDP treated SH-SY5Y-αSyn cells. Experiments were repeated independently three times (n =3). (d and e) Quantification data from a time course PLA on SH-SY5Y-αSyn cells treated with PAR, PAR + 1 μM PDD00017273 (PARGi) or ADP-HDP for 4 h (d) and 24 h (e). Bars represent means ± SEM. Two-way ANOVA followed by Tukey’s post hoc test (n =3). ****P
    Human A53t Variant αsyn, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human a53t variant αsyn/product/The Jackson Laboratory
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human a53t variant αsyn - by Bioz Stars, 2020-09
    84/100 stars
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    Image Search Results


    Intracellular effects of PAR on αSyn. (a) Cytotoxicity assay on SH-SY5Y neuroblastoma cells treated in triplicate with three different concentrations of PAR polymer for 48 h. (b) Hela cells transfected with EGFP-αSyn-A53T and treated with PAR (50 nM) for 48 h; representative ROIs from merge channel images showing αSyn A53T-EGFP aggregates (green) and increased superoxide levels i.e. higher red intensity, in the PAR treated vs. BioPORTER alone (vehicle control) cells. Images were captured using Zeiss Axio Widefield (20x/0.8) microscope. Experiments were performed in triplicate. (c) Representative Western blot and graph of γH2AX levels in PAR, PAR + 1 μM PDD00017273 (PARGi) and ADP-HDP treated SH-SY5Y-αSyn cells. Experiments were repeated independently three times (n =3). (d and e) Quantification data from a time course PLA on SH-SY5Y-αSyn cells treated with PAR, PAR + 1 μM PDD00017273 (PARGi) or ADP-HDP for 4 h (d) and 24 h (e). Bars represent means ± SEM. Two-way ANOVA followed by Tukey’s post hoc test (n =3). ****P

    Journal: bioRxiv

    Article Title: Poly (ADP-ribose) induces α-synuclein aggregation in neuronal-like cells and interacts with phosphorylated α-synuclein in post mortem PD samples

    doi: 10.1101/2020.04.08.032250

    Figure Lengend Snippet: Intracellular effects of PAR on αSyn. (a) Cytotoxicity assay on SH-SY5Y neuroblastoma cells treated in triplicate with three different concentrations of PAR polymer for 48 h. (b) Hela cells transfected with EGFP-αSyn-A53T and treated with PAR (50 nM) for 48 h; representative ROIs from merge channel images showing αSyn A53T-EGFP aggregates (green) and increased superoxide levels i.e. higher red intensity, in the PAR treated vs. BioPORTER alone (vehicle control) cells. Images were captured using Zeiss Axio Widefield (20x/0.8) microscope. Experiments were performed in triplicate. (c) Representative Western blot and graph of γH2AX levels in PAR, PAR + 1 μM PDD00017273 (PARGi) and ADP-HDP treated SH-SY5Y-αSyn cells. Experiments were repeated independently three times (n =3). (d and e) Quantification data from a time course PLA on SH-SY5Y-αSyn cells treated with PAR, PAR + 1 μM PDD00017273 (PARGi) or ADP-HDP for 4 h (d) and 24 h (e). Bars represent means ± SEM. Two-way ANOVA followed by Tukey’s post hoc test (n =3). ****P

    Article Snippet: Transfection of Hela cells with EGFP-αSyn-A53T Hela cells were transiently transfected using Lipofectamine 3000 (Invitrogen) with a pEGFP-C1 mammalian vector containing full length human A53T variant αSyn with a fusion EGFP tag (Addgene, Plasmid #40823), following the manufacturer’s protocol.

    Techniques: Cytotoxicity Assay, Transfection, Microscopy, Western Blot, Proximity Ligation Assay

    APOE genotypes differentially regulate αSyn aggregation and phosphorylation in A53T mice. (A-D) Total αSyn concentration was measured by ELISA in RAB, RAB+TX-100, RIPA, and SDS fractions from the brainstem of 12-month old A53T mice. A53T/EKO, n=10; A53T/E2, n=6; A53T/E3, n=9; A53T/E4, n=10. Closed symbols indicate asymptomatic mice; open symbols indicate symptomatic mice with endstage paralysis. Symbols shown in black in (D) indicate A53T/EKO and A53T/E3 samples below the limit of detection. (E-F) Phospho-αSyn concentration was measured by ELISA in RIPA and SDS fractions. Data expressed as mean ± SEM, one-way ANOVA with Tukey’s multiple comparisons test (A, B, E, F) or Kruskal-Wallis test with Dunn’s multiple comparisons test (C) . *p

    Journal: Science translational medicine

    Article Title: APOE Genotype Regulates Pathology and Disease Progression in Synucleinopathy *

    doi: 10.1126/scitranslmed.aay3069

    Figure Lengend Snippet: APOE genotypes differentially regulate αSyn aggregation and phosphorylation in A53T mice. (A-D) Total αSyn concentration was measured by ELISA in RAB, RAB+TX-100, RIPA, and SDS fractions from the brainstem of 12-month old A53T mice. A53T/EKO, n=10; A53T/E2, n=6; A53T/E3, n=9; A53T/E4, n=10. Closed symbols indicate asymptomatic mice; open symbols indicate symptomatic mice with endstage paralysis. Symbols shown in black in (D) indicate A53T/EKO and A53T/E3 samples below the limit of detection. (E-F) Phospho-αSyn concentration was measured by ELISA in RIPA and SDS fractions. Data expressed as mean ± SEM, one-way ANOVA with Tukey’s multiple comparisons test (A, B, E, F) or Kruskal-Wallis test with Dunn’s multiple comparisons test (C) . *p

    Article Snippet: Animals A53T αSyn-Tg mice (The Jackson Laboratory, 006823) were crossed with human APOE2 , APOE3 , or APOE4 knock-in mice (provided by P.M. Sullivan, Duke University) or Apoe knockout mice (The Jackson Laboratory, 002052) to generate A53T mice homozygous for one of the three human alleles or completely null for Apoe.

    Techniques: Mouse Assay, Concentration Assay, Enzyme-linked Immunosorbent Assay

    Intracellular effects of PAR on αSyn. (a) Cytotoxicity assay on SH-SY5Y neuroblastoma cells treated in triplicate with three different concentrations of PAR polymer for 48 h. (b) Hela cells transfected with EGFP-αSyn-A53T and treated with PAR (50 nM) for 48 h; representative ROIs from merge channel images showing αSyn A53T-EGFP aggregates (green) and increased superoxide levels i.e. higher red intensity, in the PAR treated vs. BioPORTER alone (vehicle control) cells. Images were captured using Zeiss Axio Widefield (20x/0.8) microscope. Experiments were performed in triplicate. (c) Representative Western blot and graph of γH2AX levels in PAR, PAR + 1 μM PDD00017273 (PARGi) and ADP-HDP treated SH-SY5Y-αSyn cells. Experiments were repeated independently three times (n =3). (d and e) Quantification data from a time course PLA on SH-SY5Y-αSyn cells treated with PAR, PAR + 1 μM PDD00017273 (PARGi) or ADP-HDP for 4 h (d) and 24 h (e). Bars represent means ± SEM. Two-way ANOVA followed by Tukey’s post hoc test (n =3). ****P

    Journal: bioRxiv

    Article Title: Poly (ADP-ribose) induces α-synuclein aggregation in neuronal-like cells and interacts with phosphorylated α-synuclein in post mortem PD samples

    doi: 10.1101/2020.04.08.032250

    Figure Lengend Snippet: Intracellular effects of PAR on αSyn. (a) Cytotoxicity assay on SH-SY5Y neuroblastoma cells treated in triplicate with three different concentrations of PAR polymer for 48 h. (b) Hela cells transfected with EGFP-αSyn-A53T and treated with PAR (50 nM) for 48 h; representative ROIs from merge channel images showing αSyn A53T-EGFP aggregates (green) and increased superoxide levels i.e. higher red intensity, in the PAR treated vs. BioPORTER alone (vehicle control) cells. Images were captured using Zeiss Axio Widefield (20x/0.8) microscope. Experiments were performed in triplicate. (c) Representative Western blot and graph of γH2AX levels in PAR, PAR + 1 μM PDD00017273 (PARGi) and ADP-HDP treated SH-SY5Y-αSyn cells. Experiments were repeated independently three times (n =3). (d and e) Quantification data from a time course PLA on SH-SY5Y-αSyn cells treated with PAR, PAR + 1 μM PDD00017273 (PARGi) or ADP-HDP for 4 h (d) and 24 h (e). Bars represent means ± SEM. Two-way ANOVA followed by Tukey’s post hoc test (n =3). ****P

    Article Snippet: Animals M83-SNCA*A53T mice expressing human A53T variant αSyn were obtained from The Jackson Laboratory, Bar Harbor, ME (JAX stock #004479).

    Techniques: Cytotoxicity Assay, Transfection, Microscopy, Western Blot, Proximity Ligation Assay