a31 fibroblast cell line Search Results


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  • 96
    ATCC a31 fibroblast cell line
    Characterization of nucleosomes used in this study and spontaneous splenocyte proliferation. Immunohistochemistry analysis detecting CCR2, IL1 β , and TLR7 protein expression in kidneys of 31 w.o heparin or untreated B/W mice (a). SDS PAGE of nucleosomes and HMGB1 used in cell culture stimulations ((b), lane 1: MW standard, lane 2: nucleosomes (3 μ g measured as DNA), and lane 3: HMGB1 (1 μ g)). Western blot showing HMGB1 (arrows) in nucleosomes purified from <t>A31</t> cell line ((c), lane 1: MW standard, lane 2: HMGB1 (0.1 μ g), and lane 3: nucleosomes (2 μ g measured as DNA)). Agarose gel electrophoresis of nucleosomes showing a distribution of nucleosome sizes ((d), lane 1: MW standard, lane 2: nucleosomes (3 μ g measured as DNA)). Stimulation index (SI) of cells from prenephritic mice (e) and nephritic mice (f) stimulated with conA with or without heparin. Splenocytes from nephritic mice have a significantly higher proliferation (counts per minute, CPM) at 20 hours which persisted over time compared to splenocytes from prenephritic mice (g). Mean values and SD were calculated from triplicates from each cell culture. *Statistically significant ( P
    A31 Fibroblast Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    ATCC cell culture mouse fibroblast cell lines
    Characterization of nucleosomes used in this study and spontaneous splenocyte proliferation. Immunohistochemistry analysis detecting CCR2, IL1 β , and TLR7 protein expression in kidneys of 31 w.o heparin or untreated B/W mice (a). SDS PAGE of nucleosomes and HMGB1 used in cell culture stimulations ((b), lane 1: MW standard, lane 2: nucleosomes (3 μ g measured as DNA), and lane 3: HMGB1 (1 μ g)). Western blot showing HMGB1 (arrows) in nucleosomes purified from <t>A31</t> cell line ((c), lane 1: MW standard, lane 2: HMGB1 (0.1 μ g), and lane 3: nucleosomes (2 μ g measured as DNA)). Agarose gel electrophoresis of nucleosomes showing a distribution of nucleosome sizes ((d), lane 1: MW standard, lane 2: nucleosomes (3 μ g measured as DNA)). Stimulation index (SI) of cells from prenephritic mice (e) and nephritic mice (f) stimulated with conA with or without heparin. Splenocytes from nephritic mice have a significantly higher proliferation (counts per minute, CPM) at 20 hours which persisted over time compared to splenocytes from prenephritic mice (g). Mean values and SD were calculated from triplicates from each cell culture. *Statistically significant ( P
    Cell Culture Mouse Fibroblast Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    ATCC balb c 3t3 a31 fibroblasts
    Characterization of nucleosomes used in this study and spontaneous splenocyte proliferation. Immunohistochemistry analysis detecting CCR2, IL1 β , and TLR7 protein expression in kidneys of 31 w.o heparin or untreated B/W mice (a). SDS PAGE of nucleosomes and HMGB1 used in cell culture stimulations ((b), lane 1: MW standard, lane 2: nucleosomes (3 μ g measured as DNA), and lane 3: HMGB1 (1 μ g)). Western blot showing HMGB1 (arrows) in nucleosomes purified from <t>A31</t> cell line ((c), lane 1: MW standard, lane 2: HMGB1 (0.1 μ g), and lane 3: nucleosomes (2 μ g measured as DNA)). Agarose gel electrophoresis of nucleosomes showing a distribution of nucleosome sizes ((d), lane 1: MW standard, lane 2: nucleosomes (3 μ g measured as DNA)). Stimulation index (SI) of cells from prenephritic mice (e) and nephritic mice (f) stimulated with conA with or without heparin. Splenocytes from nephritic mice have a significantly higher proliferation (counts per minute, CPM) at 20 hours which persisted over time compared to splenocytes from prenephritic mice (g). Mean values and SD were calculated from triplicates from each cell culture. *Statistically significant ( P
    Balb C 3t3 A31 Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    ATCC mouse fibroblast balb c 3t3 cell lines
    Treatment of Manilkara zapota leaf methanol extract on cancer cells. (a) Treatment of Manilkara zapota leaf methanol extract on HeLa cells. The cell viability was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay after 72 h exposure with Manilkara zapota leaf methanol extract. (b) Manilkara zapota leaf methanol extract increases proliferation of human prostate cancer (PC-3) cells after 24, 48, and 72 h using MTT assay. (c) Manilkara zapota leaf methanol extract promotes proliferation of PC-3 cells after 24, 48, and 72 h evaluated by lactate dehydrogenase (LDH) assay. (d) Treatment of Manilkara zapota leaf methanol extract in mouse fibroblast <t>(BALB/c</t> <t>3T3)</t> cell lines evaluated using MTT assay. (e) Cell viability of BALB/c 3T3 cell lines after treatment with Manilkara zapota leaf methanol extract was evaluated using LDH assay. Values are reported as mean ± SD (n = 3). Value with different superscript letter indicates significant difference between groups by Tukey's test ( P
    Mouse Fibroblast Balb C 3t3 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Characterization of nucleosomes used in this study and spontaneous splenocyte proliferation. Immunohistochemistry analysis detecting CCR2, IL1 β , and TLR7 protein expression in kidneys of 31 w.o heparin or untreated B/W mice (a). SDS PAGE of nucleosomes and HMGB1 used in cell culture stimulations ((b), lane 1: MW standard, lane 2: nucleosomes (3 μ g measured as DNA), and lane 3: HMGB1 (1 μ g)). Western blot showing HMGB1 (arrows) in nucleosomes purified from A31 cell line ((c), lane 1: MW standard, lane 2: HMGB1 (0.1 μ g), and lane 3: nucleosomes (2 μ g measured as DNA)). Agarose gel electrophoresis of nucleosomes showing a distribution of nucleosome sizes ((d), lane 1: MW standard, lane 2: nucleosomes (3 μ g measured as DNA)). Stimulation index (SI) of cells from prenephritic mice (e) and nephritic mice (f) stimulated with conA with or without heparin. Splenocytes from nephritic mice have a significantly higher proliferation (counts per minute, CPM) at 20 hours which persisted over time compared to splenocytes from prenephritic mice (g). Mean values and SD were calculated from triplicates from each cell culture. *Statistically significant ( P

    Journal: Clinical and Developmental Immunology

    Article Title: LMW Heparin Prevents Increased Kidney Expression of Proinflammatory Mediators in (NZBxNZW)F1 Mice

    doi: 10.1155/2013/791262

    Figure Lengend Snippet: Characterization of nucleosomes used in this study and spontaneous splenocyte proliferation. Immunohistochemistry analysis detecting CCR2, IL1 β , and TLR7 protein expression in kidneys of 31 w.o heparin or untreated B/W mice (a). SDS PAGE of nucleosomes and HMGB1 used in cell culture stimulations ((b), lane 1: MW standard, lane 2: nucleosomes (3 μ g measured as DNA), and lane 3: HMGB1 (1 μ g)). Western blot showing HMGB1 (arrows) in nucleosomes purified from A31 cell line ((c), lane 1: MW standard, lane 2: HMGB1 (0.1 μ g), and lane 3: nucleosomes (2 μ g measured as DNA)). Agarose gel electrophoresis of nucleosomes showing a distribution of nucleosome sizes ((d), lane 1: MW standard, lane 2: nucleosomes (3 μ g measured as DNA)). Stimulation index (SI) of cells from prenephritic mice (e) and nephritic mice (f) stimulated with conA with or without heparin. Splenocytes from nephritic mice have a significantly higher proliferation (counts per minute, CPM) at 20 hours which persisted over time compared to splenocytes from prenephritic mice (g). Mean values and SD were calculated from triplicates from each cell culture. *Statistically significant ( P

    Article Snippet: The cells were incubated with different stimulators (all in triplicates): nucleosomes (10 μ g/mL) prepared from the murine BALB/c 3T3 clone A31 fibroblast cell line (ATCC CCL-163) and characterized as previously described in [ ], nucleosomes (10 μ g/mL) together with LMW heparin (Enoxaparin, Klexane, Aventis Pharma AS, Oslo, Norway, 24 μ g/mL) at a molar ratio of 1 : 100 (nucleosomes (determined as core nucleosome equivalents): heparin), LMW heparin (24 μ g/mL), concanavalin A (con A) (2.5 μ g/mL) (Sigma-Aldrich), and conA with LMW heparin (1 : 200) and HMGB1 (1 μ g/mL) (Sigma-Aldrich).

    Techniques: Immunohistochemistry, Expressing, Mouse Assay, SDS Page, Cell Culture, Western Blot, Purification, Agarose Gel Electrophoresis

    DABL compounds inhibit mouse polyoma virus. (A) A31 cells uninfected or infected with mouse MuPyV and treated with increasing concentrations (100 nM, 1.0 μM or 10 μM) of the indicated compounds. (B) Total RNA was isolated 24 hpi from cells treated with the indicated concentration of compound. Levels of MuPyV LT mRNA were determined by quantitative PCR and normalized to untreated cells. Shown is mean (± SD), data is cumulative from two independent experiments (n=4 per treatment condition). Statistical significance was determined by 2-way ANOVA (*, **, **** p

    Journal: Bioorganic & medicinal chemistry

    Article Title: Inhibition of Diverse Opportunistic Viruses by Structurally Optimized Retrograde Trafficking Inhibitors

    doi: 10.1016/j.bmc.2019.03.026

    Figure Lengend Snippet: DABL compounds inhibit mouse polyoma virus. (A) A31 cells uninfected or infected with mouse MuPyV and treated with increasing concentrations (100 nM, 1.0 μM or 10 μM) of the indicated compounds. (B) Total RNA was isolated 24 hpi from cells treated with the indicated concentration of compound. Levels of MuPyV LT mRNA were determined by quantitative PCR and normalized to untreated cells. Shown is mean (± SD), data is cumulative from two independent experiments (n=4 per treatment condition). Statistical significance was determined by 2-way ANOVA (*, **, **** p

    Article Snippet: Normal human dermal fibroblasts (HDFs) (Cell Applications Inc., 106-05N), normal human lung fibroblasts (MRC-5) (ATCC, CCL-171), BALB/3T3 clone A31 mouse fibroblasts (A31) (ATCC, CCL-163) and African green monkey kidney epithelial cells (VERO) were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Lonza).

    Techniques: Infection, Isolation, Concentration Assay, Real-time Polymerase Chain Reaction

    Infected-wound healing using nFeS regimen. a Morphology of P. aeruginosa before (control) and after Cys-nFeS treatment. The red triangles indicate flagella. Scale bars: 1 µm. b Photographs of P. aeruginosa infected wounds treated with buffer (control), Cys-nFeS, H 2 O 2 , and Cys-nFeS + H 2 O 2 at different times (five mice in each group). c Histologic analyses of the corresponding treated wounds in ( b ) at day 6. Scale bars: 500 µm. d Influence of Cys-nFeS on the viability of fibroblast cells. e Influence of the supernatant from Cys-nFeS on the viability of fibroblast cells. f Stimulation of fibroblast cell proliferation by Fe 3 O 4 nanoparticles. Data are shown as the mean ± s.d. Statistical significance was assessed using an unpaired Student’s two-sided t -test compared to the control group. ** p

    Journal: Nature Communications

    Article Title: Converting organosulfur compounds to inorganic polysulfides against resistant bacterial infections

    doi: 10.1038/s41467-018-06164-7

    Figure Lengend Snippet: Infected-wound healing using nFeS regimen. a Morphology of P. aeruginosa before (control) and after Cys-nFeS treatment. The red triangles indicate flagella. Scale bars: 1 µm. b Photographs of P. aeruginosa infected wounds treated with buffer (control), Cys-nFeS, H 2 O 2 , and Cys-nFeS + H 2 O 2 at different times (five mice in each group). c Histologic analyses of the corresponding treated wounds in ( b ) at day 6. Scale bars: 500 µm. d Influence of Cys-nFeS on the viability of fibroblast cells. e Influence of the supernatant from Cys-nFeS on the viability of fibroblast cells. f Stimulation of fibroblast cell proliferation by Fe 3 O 4 nanoparticles. Data are shown as the mean ± s.d. Statistical significance was assessed using an unpaired Student’s two-sided t -test compared to the control group. ** p

    Article Snippet: Cell viability assay of fibroblast cells Embryonic mouse fibroblasts (BALB/3T3 clone A31, ATCC CCL-163) were seeded into sterile 96-well plates at 104 well−1 in the medium (DMEM with 10% FBS) and followed to attach for 24 h at 37 °C under 5% CO2 .

    Techniques: Infection, Mouse Assay

    AB assay for determination of BALB 3T3 fibroblasts in vitro proliferation on PHACOS (gray bars) and PHO surfaces (black bars). White bars represent PET control.

    Journal: Biomaterials

    Article Title: PHACOS, a functionalized bacterial polyester with bactericidal activity against methicillin-resistant Staphylococcus aureus

    doi: 10.1016/j.biomaterials.2013.09.059

    Figure Lengend Snippet: AB assay for determination of BALB 3T3 fibroblasts in vitro proliferation on PHACOS (gray bars) and PHO surfaces (black bars). White bars represent PET control.

    Article Snippet: Cellular toxicity was evaluated using either murine RAW 264.7 macrophages (ECACC, Sigma P11) or BALB 3T3 fibroblasts (ATCC, CCL-163, P12), while inflammatory activity was monitored only on RAW 264.7 cells and effects on cell proliferation only on fibroblasts BALB 3T3.

    Techniques: In Vitro, Positron Emission Tomography

    Recombinant IFN-γ-induced Ia expression and allostimulatory activity by 12E2 cells in vitro . A to C: 12E2 cells were negative to weakly positive by immunohistochemistry for Ia ( A ), whereas exposure to increasing concentrations of recombinant IFN-γ induced progressively intense immunoreactivity ( B and C ). D: Fixed numbers of naïve B10.BR T cells were combined with decreasing numbers of the respective stimulator cell type (BALB/c-positive control splenocytes (□), unstimulated 12E2 cells (○), 12E2 cells with IFN-γ pretreatment (▪), and IFN-γ-pretreated 3T3-negative control murine fibroblasts (•) to attain the indicated stimulator:responder ratios. Proliferation was measured on day 4 of co-culture and the stimulation index (SI) was calculated (see Materials and Methods). Optimal BALB/c allogeneic control stimulation at higher stimulator:responder cell ratios is shown. However, correlative data could not be generated for 12E2 cells as a result of cell aggregation at these higher ratios. Original magnification, ×400 with NovaRed chromagen ( A to C ).

    Journal: The American Journal of Pathology

    Article Title: 12E2

    doi:

    Figure Lengend Snippet: Recombinant IFN-γ-induced Ia expression and allostimulatory activity by 12E2 cells in vitro . A to C: 12E2 cells were negative to weakly positive by immunohistochemistry for Ia ( A ), whereas exposure to increasing concentrations of recombinant IFN-γ induced progressively intense immunoreactivity ( B and C ). D: Fixed numbers of naïve B10.BR T cells were combined with decreasing numbers of the respective stimulator cell type (BALB/c-positive control splenocytes (□), unstimulated 12E2 cells (○), 12E2 cells with IFN-γ pretreatment (▪), and IFN-γ-pretreated 3T3-negative control murine fibroblasts (•) to attain the indicated stimulator:responder ratios. Proliferation was measured on day 4 of co-culture and the stimulation index (SI) was calculated (see Materials and Methods). Optimal BALB/c allogeneic control stimulation at higher stimulator:responder cell ratios is shown. However, correlative data could not be generated for 12E2 cells as a result of cell aggregation at these higher ratios. Original magnification, ×400 with NovaRed chromagen ( A to C ).

    Article Snippet: Murine fibroblast BALB/3T3 (clone A31), keratinocyte PAM212, and endothelial 3B-11 cell lines (ATCC, American Type Culture Collection, Manassas, VA) were grown according to ATCC protocols.

    Techniques: Recombinant, IA, Expressing, Activity Assay, In Vitro, Immunohistochemistry, Positive Control, Negative Control, Co-Culture Assay, Generated

    Cell viability of mouse fibroblasts. Effect of different concentrations (1 – 1000 μg/ml) of freeze-dried Echinacea suspension (ES) on cell viability of mouse fibroblasts cultured in Dulbecco’s Modified Eagle Medium (DMEM), and incubated with re-suspended freeze dried ES for 16 h. Oxidative stress was artificially induced by incubating cultured cells with 150 μM hydroxide peroxide for 2 h. The percentage of viable cells with respect to untreated cultures was measured through the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. ES fermented for 24 h at 30°C with Lactobacillus plantarum 1MR20 (1MR20-ES), C2 (C2-ES) and POM1 (POM1-ES), and with the association between strains 1MR20 and C2 (1MR20/C2-ES) were assayed. ES-CT, chemically acidified ES, without bacterial inoculum, and incubated for 24 h at 30°C, was the control. α-Tocopherol (α-t1, 250 μg/ml, and α-t2, 500 μg/ml) was used as the positive control. Data are the means of three independent experiments twice analysed.

    Journal: Microbial Cell Factories

    Article Title: Lactic acid fermentation as a tool to enhance the functional features of Echinacea spp

    doi: 10.1186/1475-2859-12-44

    Figure Lengend Snippet: Cell viability of mouse fibroblasts. Effect of different concentrations (1 – 1000 μg/ml) of freeze-dried Echinacea suspension (ES) on cell viability of mouse fibroblasts cultured in Dulbecco’s Modified Eagle Medium (DMEM), and incubated with re-suspended freeze dried ES for 16 h. Oxidative stress was artificially induced by incubating cultured cells with 150 μM hydroxide peroxide for 2 h. The percentage of viable cells with respect to untreated cultures was measured through the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. ES fermented for 24 h at 30°C with Lactobacillus plantarum 1MR20 (1MR20-ES), C2 (C2-ES) and POM1 (POM1-ES), and with the association between strains 1MR20 and C2 (1MR20/C2-ES) were assayed. ES-CT, chemically acidified ES, without bacterial inoculum, and incubated for 24 h at 30°C, was the control. α-Tocopherol (α-t1, 250 μg/ml, and α-t2, 500 μg/ml) was used as the positive control. Data are the means of three independent experiments twice analysed.

    Article Snippet: Viability of oxidation-induced cells Mouse fibroblasts (Balb 3T3, clone A31, ATCC CCL-163TM) were cultured under humidified atmosphere (5% CO2 , 37°C), using Dulbecco’s Modified Eagle Medium (DMEM), which was supplemented with 10% (w/v) calf bovine serum (CBS), 1% penicillin (10,000 U/mL)/streptomycin (10,000 U/mL) mixture, and 1% non-essential amino acid solution (NEAA).

    Techniques: Cell Culture, Modification, Incubation, MTT Assay, Positive Control

    WST-1 cell proliferation assay performed on PCL extracts in solution with 3T3/A31 fibroblasts: (a) processed PCL extracts; (b) nonprocessed PCL extracts.

    Journal: International Journal of Biomaterials

    Article Title: Polycaprolactone Scaffolds Fabricated via Bioextrusion for Tissue Engineering Applications

    doi: 10.1155/2009/239643

    Figure Lengend Snippet: WST-1 cell proliferation assay performed on PCL extracts in solution with 3T3/A31 fibroblasts: (a) processed PCL extracts; (b) nonprocessed PCL extracts.

    Article Snippet: Materials Cell line BALB/3T3 Clone A31 mouse embryo fibroblasts (CCL163) was obtained from American Type Culture Collection (ATCC) and propagated as indicated by the supplier.

    Techniques: Proliferation Assay

    Treatment of Manilkara zapota leaf methanol extract on cancer cells. (a) Treatment of Manilkara zapota leaf methanol extract on HeLa cells. The cell viability was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay after 72 h exposure with Manilkara zapota leaf methanol extract. (b) Manilkara zapota leaf methanol extract increases proliferation of human prostate cancer (PC-3) cells after 24, 48, and 72 h using MTT assay. (c) Manilkara zapota leaf methanol extract promotes proliferation of PC-3 cells after 24, 48, and 72 h evaluated by lactate dehydrogenase (LDH) assay. (d) Treatment of Manilkara zapota leaf methanol extract in mouse fibroblast (BALB/c 3T3) cell lines evaluated using MTT assay. (e) Cell viability of BALB/c 3T3 cell lines after treatment with Manilkara zapota leaf methanol extract was evaluated using LDH assay. Values are reported as mean ± SD (n = 3). Value with different superscript letter indicates significant difference between groups by Tukey's test ( P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: ROS-Mediated Mitochondrial Pathway is Required for Manilkara Zapota (L.) P. Royen Leaf Methanol Extract Inducing Apoptosis in the Modulation of Caspase Activation and EGFR/NF-κB Activities of HeLa Human Cervical Cancer Cells

    doi: 10.1155/2018/6578648

    Figure Lengend Snippet: Treatment of Manilkara zapota leaf methanol extract on cancer cells. (a) Treatment of Manilkara zapota leaf methanol extract on HeLa cells. The cell viability was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay after 72 h exposure with Manilkara zapota leaf methanol extract. (b) Manilkara zapota leaf methanol extract increases proliferation of human prostate cancer (PC-3) cells after 24, 48, and 72 h using MTT assay. (c) Manilkara zapota leaf methanol extract promotes proliferation of PC-3 cells after 24, 48, and 72 h evaluated by lactate dehydrogenase (LDH) assay. (d) Treatment of Manilkara zapota leaf methanol extract in mouse fibroblast (BALB/c 3T3) cell lines evaluated using MTT assay. (e) Cell viability of BALB/c 3T3 cell lines after treatment with Manilkara zapota leaf methanol extract was evaluated using LDH assay. Values are reported as mean ± SD (n = 3). Value with different superscript letter indicates significant difference between groups by Tukey's test ( P

    Article Snippet: Cell Culture The human colon carcinoma (HCT-116), human colorectal adenocarcinoma (HT-29), human cervical cancer (HeLa), human hepatocellular carcinoma (HepG2), human gastric adenocarcinoma (HGT-1), human prostate cancer (PC-3), and mouse fibroblast (BALB/c 3T3) cell lines were obtained from American Type Culture Collection (ATCC; Rockville, MD, USA).

    Techniques: MTT Assay, Lactate Dehydrogenase Assay