Journal: Cell Death & Disease

Article Title: Targeting autophagy enhances atezolizumab-induced mitochondria-related apoptosis in osteosarcoma

doi: 10.1038/s41419-021-03449-6

Figure Lengend Snippet: A , B Proliferation of HOS and 143B was detected by CCK-8 assay after the treatment of atezolizumab with different concentrations; C IC 50 of HOS and 143B to atezolizumab; D , E Proliferation of HOS and 143B was detected by colony formation assay after the treatment of atezolizumab; F , G Apoptosis of HOS and 143B was detected by flow cytometry after the treatment of atezolizumab. All data are from at least three independent experiments and are presented as the means ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: Different concentrations of atezolizumab (A2004, Selleck, USA) were added to the medium.

Techniques: CCK-8 Assay, Colony Assay, Flow Cytometry

Journal: Cell Death & Disease

Article Title: Targeting autophagy enhances atezolizumab-induced mitochondria-related apoptosis in osteosarcoma

doi: 10.1038/s41419-021-03449-6

Figure Lengend Snippet: A Transmission electron microscopy of HOS and 143B after the treatment of atezolizumab (10 µg/ml) (red arrows point to mitochondria); B Mitochondrial membrane potential of HOS and 143B was detected by JC-1 staining after the treatment of atezolizumab (normal OS cells which have high ΔΨm show strong red fluorescence and weak green fluorescence); C Quantification of JC-1 staining in HOS and 143B; D , E Release of ROS in HOS and 143B was detected by flow cytometry after the treatment of atezolizumab; F Amount of SOD in HOS and 143B was detected by the total SOD assay kit. All data are from at least three independent experiments and are presented as the means ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: Different concentrations of atezolizumab (A2004, Selleck, USA) were added to the medium.

Techniques: Transmission Assay, Electron Microscopy, Staining, Fluorescence, Flow Cytometry

Journal: Cell Death & Disease

Article Title: Targeting autophagy enhances atezolizumab-induced mitochondria-related apoptosis in osteosarcoma

doi: 10.1038/s41419-021-03449-6

Figure Lengend Snippet: A – D Expression of mitochondria-related apoptosis protein in HOS and 143B was evaluated by western blot after the treatment of atezolizumab (10 µg/ml) with or without SP600125 (10 μM); E Representative immunofluorescence staining images of cleaved-caspase-9 (green) and cleaved-caspase-3 (red) in HOS and 143B after the treatment of atezolizumab with or without SP600125; F – I Expression of phospho-JNK and JNK in HOS and 143B was detected by western blot after the treatment of atezolizumab with different concentrations. All data are from at least three independent experiments and are presented as the means ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: Different concentrations of atezolizumab (A2004, Selleck, USA) were added to the medium.

Techniques: Expressing, Western Blot, Immunofluorescence, Staining

Journal: Cell Death & Disease

Article Title: Targeting autophagy enhances atezolizumab-induced mitochondria-related apoptosis in osteosarcoma

doi: 10.1038/s41419-021-03449-6

Figure Lengend Snippet: A , B Expression of autophagy-related protein in HOS and 143B was evaluated by western blot after the treatment of atezolizumab with different concentrations; C , D Transmission electron microscopy of HOS and 143B after the treatment of atezolizumab (10 µg/ml) for 24 h; E , F Representative immunofluorescence staining images of LC3B in HOS and 143B after the treatment of atezolizumab (10 µg/ml) with or without mito-TEMPO (10 μM); G , H Expression of autophagy-related protein in HOS and 143B was evaluated by western blot after the treatment of atezolizumab with or without mito-TEMPO; I , J Representative images and analysis of GFP-RFP-LC3 transfected HOS and 143B after the treatment of atezolizumab with or without mito-TEMPO. All data are from at least three independent experiments and are presented as the means ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: Different concentrations of atezolizumab (A2004, Selleck, USA) were added to the medium.

Techniques: Expressing, Western Blot, Transmission Assay, Electron Microscopy, Immunofluorescence, Staining, Transfection

Journal: Cell Death & Disease

Article Title: Targeting autophagy enhances atezolizumab-induced mitochondria-related apoptosis in osteosarcoma

doi: 10.1038/s41419-021-03449-6

Figure Lengend Snippet: A Representative immunofluorescence staining images of cleaved-caspase-3 in HOS and 143B after the treatment of atezolizumab (10 µg/ml) with or without CQ (10 μM); B , C Expression of apoptosis-related protein in HOS and 143B was detected by western blot after the treatment of atezolizumab with or without CQ; D , E Apoptosis of HOS and 143B was detected by flow cytometry after the treatment of atezolizumab with or without CQ; F , G Representative images and quantification of TUNEL staining in HOS and 143B after the treatment of atezolizumab with or without CQ. All data are from at least three independent experiments and are presented as the means ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: Different concentrations of atezolizumab (A2004, Selleck, USA) were added to the medium.

Techniques: Immunofluorescence, Staining, Expressing, Western Blot, Flow Cytometry, TUNEL Assay