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  • 95
    ATCC a actinomycetemcomitans
    Effect of the Nano-MIX on the multi-species biofilms of S. mutans , F. nucleatum , A. <t>actinomycetemcomitans</t> , and P. gingivalis at 24 h. The confocal scanning laser microscopy (CLSM) ( A , B ) and scanning electron microscopy (SEM) images ( C , D ) showing comparative antibacterial effects of the Nano-MIX treatment ( B , D ) on the mixed-species oral biofilms with reference to the blank nanoparticles ( A , C ), respectively.
    A Actinomycetemcomitans, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 546 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    ATCC actinobacillus actinomycetemcomitans
    Activation of caspase-3 by treatment of HL-60 cells in the presence of periodontopathic bacterial extracts. Ten micrograms of cellular lysate prepared from HL-60 cells (5 × 10 5 cells) which had been treated with 1 μg of each bacterial extract per ml or with PBS was subjected to Western blot analysis by using anti-procaspase-3 antibody. Lanes: 1, A. <t>actinomycetemcomitans</t> serotype a; 2, A. actinomycetemcomitans serotype b; 3, A. actinomycetemcomitans serotype c; 4, B. forsythus ; 5, E. coli ; 6, PBS). Decreases in procaspase-3 levels represent the activation of caspase-3. The filter was also probed with anti-tubulin antibody to monitor the amount of loading in each lane.
    Actinobacillus Actinomycetemcomitans, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC e coli atcc 29522
    Effects of 80 AU ml −1  of purified antimicrobial peptide AN5-1 produced by  P. alvei  AN5 on early exponential growth phase against target strains:  a E. coli  ATCC 29522,  b S. marcescens ,  c S. aureus  and  d B. cereus  ATCC 14579, in the absence ( filled circle ) and presence ( filled triangle ) of antimicrobial peptide AN5-1. The bacterial growth was measured by means of optical density at 600 nm (OD 600 )
    E Coli Atcc 29522, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    ATCC a actinomycetemcomitans atcc 33384
    Inhibition (a) and eracdication (b) of biofilm formation of S. mutans ATCC 25175 (□) and A. <t>actinomycetemcomitans</t> <t>ATCC</t> 33384 (■) by P. betle leaves extract at various concentrations, 0.1% (w/v) CHX was used as control. Error bars indicate standard deviations; n = 6.
    A Actinomycetemcomitans Atcc 33384, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Zambon j j actinobacillus actinomycetemcomitans
    Inhibition (a) and eracdication (b) of biofilm formation of S. mutans ATCC 25175 (□) and A. <t>actinomycetemcomitans</t> <t>ATCC</t> 33384 (■) by P. betle leaves extract at various concentrations, 0.1% (w/v) CHX was used as control. Error bars indicate standard deviations; n = 6.
    J J Actinobacillus Actinomycetemcomitans, supplied by Zambon, used in various techniques. Bioz Stars score: 88/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC a actinomycetemcomitans atcc 29523
    Adhesion of wild-type A. <t>actinomycetemcomitans</t> and Aae mutant strains to KB monolayers. SUNY 465 and <t>ATCC</t> 29523 strains were used. Experiments were performed with quadruplicate wells for each strain. Results shown are from a typical experiment; bars represent the standard deviation of the replicates.
    A Actinomycetemcomitans Atcc 29523, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    DSMZ a actinomycetemcomitans
    Adhesion of wild-type A. <t>actinomycetemcomitans</t> and Aae mutant strains to KB monolayers. SUNY 465 and <t>ATCC</t> 29523 strains were used. Experiments were performed with quadruplicate wells for each strain. Results shown are from a typical experiment; bars represent the standard deviation of the replicates.
    A Actinomycetemcomitans, supplied by DSMZ, used in various techniques. Bioz Stars score: 92/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    ATCC atcc 43719tm
    Adhesion of wild-type A. <t>actinomycetemcomitans</t> and Aae mutant strains to KB monolayers. SUNY 465 and <t>ATCC</t> 29523 strains were used. Experiments were performed with quadruplicate wells for each strain. Results shown are from a typical experiment; bars represent the standard deviation of the replicates.
    Atcc 43719tm, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Effect of the Nano-MIX on the multi-species biofilms of S. mutans , F. nucleatum , A. actinomycetemcomitans , and P. gingivalis at 24 h. The confocal scanning laser microscopy (CLSM) ( A , B ) and scanning electron microscopy (SEM) images ( C , D ) showing comparative antibacterial effects of the Nano-MIX treatment ( B , D ) on the mixed-species oral biofilms with reference to the blank nanoparticles ( A , C ), respectively.

    Journal: Nanomaterials

    Article Title: Synergistic Antibacterial Effects of Nanoparticles Encapsulated with Scutellaria baicalensis and Pure Chlorhexidine on Oral Bacterial Biofilms

    doi: 10.3390/nano6040061

    Figure Lengend Snippet: Effect of the Nano-MIX on the multi-species biofilms of S. mutans , F. nucleatum , A. actinomycetemcomitans , and P. gingivalis at 24 h. The confocal scanning laser microscopy (CLSM) ( A , B ) and scanning electron microscopy (SEM) images ( C , D ) showing comparative antibacterial effects of the Nano-MIX treatment ( B , D ) on the mixed-species oral biofilms with reference to the blank nanoparticles ( A , C ), respectively.

    Article Snippet: Anti-Biofilm Properties of the Nano-MIX (Nano-SB and Nano-CHX at 9:1 w/w) Representative oral bacteria including S. mutans (ATCC 35668), S. sobrinus (ATCC 33478), F. nucleatum (ATCC 25586), A. actinomycetemcomitans (ATCC 43718), E. faecalis (ATCC 29212), and P. gingivalis (ATCC 33277), derived from the archival collection at the Centralized Research Laboratory of the Faculty of Dentistry, The University of Hong Kong, were used in the present study.

    Techniques: Microscopy, Confocal Laser Scanning Microscopy, Electron Microscopy

    Compositional analysis and ultrastructural differences in multi-species oral biofilms. Bacterial DNA was extracted from multi- species biofilms using DNeasy Qiagen kit for quantification of each species using SYBR GreenER based quantitative PCR ( A – C ). Unique biofilm morphology and architecture was visualised using scanning electron microscopy (( D – F ); lower magnification ×500, and higher magnification ×5000 inset) and transmission electron microscopy (( G – I ); lower magnification ×15000, and higher magnification ×25000 inset). Confocal stacked images representative of biofilms stained with SYTO9 and PI to show live and dead cells as viewed using the Zeiss LSM 780 confocal laser scanning microscope. Confocal images were taken at ×40 magnification and image stacks compiled using COMSTAT2 program. Data for qPCR compositional analysis of biofilms representative of mean +/− SEM for n = 3 from two individual experiments. Abbreviations; A. a* = Aggregatibacter actinomycetemcomitans .

    Journal: Scientific Reports

    Article Title: Biofilm-stimulated epithelium modulates the inflammatory responses in co-cultured immune cells

    doi: 10.1038/s41598-019-52115-7

    Figure Lengend Snippet: Compositional analysis and ultrastructural differences in multi-species oral biofilms. Bacterial DNA was extracted from multi- species biofilms using DNeasy Qiagen kit for quantification of each species using SYBR GreenER based quantitative PCR ( A – C ). Unique biofilm morphology and architecture was visualised using scanning electron microscopy (( D – F ); lower magnification ×500, and higher magnification ×5000 inset) and transmission electron microscopy (( G – I ); lower magnification ×15000, and higher magnification ×25000 inset). Confocal stacked images representative of biofilms stained with SYTO9 and PI to show live and dead cells as viewed using the Zeiss LSM 780 confocal laser scanning microscope. Confocal images were taken at ×40 magnification and image stacks compiled using COMSTAT2 program. Data for qPCR compositional analysis of biofilms representative of mean +/− SEM for n = 3 from two individual experiments. Abbreviations; A. a* = Aggregatibacter actinomycetemcomitans .

    Article Snippet: Bacterial growth and standardisation Bacterial strains used in the study were as follows; Streptococcus mitis NCTC 12261, Streptococcus intermedius DSM 20753, Streptococcus oralis NTCC 11427, Fusobacterium nucleatum ATCC 10596, Fusobacterium nucleatum spp. vincentii DSM 19507, Actinomyces naeslundii DSM 17233 Veillonella dispar NCTC 11831, Porphyromonas gingivalis W83, Prevotella intermedia DSM 20706 and Aggregatibacter actinomycetemcomitans ATCC 43718.

    Techniques: Real-time Polymerase Chain Reaction, Electron Microscopy, Transmission Assay, Staining, Laser-Scanning Microscopy

    Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces naeslundii (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: In vitro Increased Respiratory Activity of Selected Oral Bacteria May Explain Competitive and Collaborative Interactions in the Oral Microbiome

    doi: 10.3389/fcimb.2017.00235

    Figure Lengend Snippet: Specific groups of metabolites influenced respiratory activity of each bacterial community. Principal Coordinate Analysis assisted to explain the grouping trends in the cluster analysis, and highlighted similarities in the respiratory activity among the selected oral bacteria at 24 h (A) and 48 h (B) . Aa, Aggregatibacter actinomycetemcomitans (ATCC 43718); Fn, Fusobacterium nucleatum (ATCC 10953); Pg, Porphyromonas gingivalis (ATCC 33277); Pi, Prevotella intermedia (ATCC 25611); Sm, Streptococcus mutans (ATCC 25175); Ssob, Streptococcus sobrinus (ATCC 33478); Tf, Tannerella forsythia (ATCC 43037); An, Actinomyces naeslundii (ATCC 51655); Csput, Capnocytophaga sputigena (ATCC 33612); Sgord, Streptococcus gordonii (ATCC 49818); Avisc, Actinomyces viscosus (ATCC 15987); Smitis, Streptococcus mitis (ATCC 49456); Vparv, Veillonella parvula (DSM 2007); Ssang, Streptococcus sanguinis (LMG 14657), and Ssal, Streptococcus salivarius strain TOVE-R.

    Article Snippet: Bacterial cultures and collection The following strains commonly present in the oral microbiome (Aas et al., ; Maddi and Scannapieco, ; Loozen et al., ) were obtained from the American Type Culture Collection (ATCC): A . actinomycetemcomitans (ATCC 43718), F. nucleatum (ATCC 10953), P. gingivalis (ATCC 33277), P. intermedia (ATCC 25611), S. mutans (ATCC 25175), S. sobrinus (ATCC 33478), T. forsythia (ATCC 43037), Actinomyces naeslundii (ATCC 51655), Capnocytophaga sputigena (ATCC 33612), Streptococcus gordonii (ATCC 49818), Actinomyces viscosus (ATCC 15987), and S. mitis (ATCC 49456).

    Techniques: Activity Assay

    Activation of caspase-3 by treatment of HL-60 cells in the presence of periodontopathic bacterial extracts. Ten micrograms of cellular lysate prepared from HL-60 cells (5 × 10 5 cells) which had been treated with 1 μg of each bacterial extract per ml or with PBS was subjected to Western blot analysis by using anti-procaspase-3 antibody. Lanes: 1, A. actinomycetemcomitans serotype a; 2, A. actinomycetemcomitans serotype b; 3, A. actinomycetemcomitans serotype c; 4, B. forsythus ; 5, E. coli ; 6, PBS). Decreases in procaspase-3 levels represent the activation of caspase-3. The filter was also probed with anti-tubulin antibody to monitor the amount of loading in each lane.

    Journal: Infection and Immunity

    Article Title: Novel Apoptosis-Inducing Activity in Bacteroides forsythus: a Comparative Study with Three Serotypes of Actinobacillus actinomycetemcomitans

    doi:

    Figure Lengend Snippet: Activation of caspase-3 by treatment of HL-60 cells in the presence of periodontopathic bacterial extracts. Ten micrograms of cellular lysate prepared from HL-60 cells (5 × 10 5 cells) which had been treated with 1 μg of each bacterial extract per ml or with PBS was subjected to Western blot analysis by using anti-procaspase-3 antibody. Lanes: 1, A. actinomycetemcomitans serotype a; 2, A. actinomycetemcomitans serotype b; 3, A. actinomycetemcomitans serotype c; 4, B. forsythus ; 5, E. coli ; 6, PBS). Decreases in procaspase-3 levels represent the activation of caspase-3. The filter was also probed with anti-tubulin antibody to monitor the amount of loading in each lane.

    Article Snippet: For comparison with B. forsythus , the same analyses were applied to two strains with different serotypes of Actinobacillus actinomycetemcomitans , serotypes a (ATCC 43717) and c (ATCC 43719), in addition to previously reported apoptosis-inducing serotype b (ATCC 43718), which was used as a positive control.

    Techniques: Activation Assay, Western Blot

    Flow cytometric analysis of HL-60 cells incubated with or without 50 μg of protein of the extracts from various bacteria per ml. Panels: A, PBS; B, adriamycin (10 μM); C, E. coli ; D, B. forsythus ; E, A. actinomycetemcomitans serotype a; F, A. actinomycetemcomitans serotype b; G, A. actinomycetemcomitans serotype c. The extent of cell death was assessed by measuring fluorescence intensity using a FACScalibur flow cytometer after staining with PI and Rh123. All analyses were performed in duplicate experiments.

    Journal: Infection and Immunity

    Article Title: Novel Apoptosis-Inducing Activity in Bacteroides forsythus: a Comparative Study with Three Serotypes of Actinobacillus actinomycetemcomitans

    doi:

    Figure Lengend Snippet: Flow cytometric analysis of HL-60 cells incubated with or without 50 μg of protein of the extracts from various bacteria per ml. Panels: A, PBS; B, adriamycin (10 μM); C, E. coli ; D, B. forsythus ; E, A. actinomycetemcomitans serotype a; F, A. actinomycetemcomitans serotype b; G, A. actinomycetemcomitans serotype c. The extent of cell death was assessed by measuring fluorescence intensity using a FACScalibur flow cytometer after staining with PI and Rh123. All analyses were performed in duplicate experiments.

    Article Snippet: For comparison with B. forsythus , the same analyses were applied to two strains with different serotypes of Actinobacillus actinomycetemcomitans , serotypes a (ATCC 43717) and c (ATCC 43719), in addition to previously reported apoptosis-inducing serotype b (ATCC 43718), which was used as a positive control.

    Techniques: Flow Cytometry, Incubation, Fluorescence, Cytometry, Staining

    Dose-dependent induction of apoptotic cell death by B. forsythus and A. actinomycetemcomitans strains. HL-60 cells were incubated in the presence of various concentrations of extracts, ranging from 0.1 to 50 μg of protein/ml for 24 or 48 h. All analyses were performed in duplicate experiments. The results shown are means and standard deviations.

    Journal: Infection and Immunity

    Article Title: Novel Apoptosis-Inducing Activity in Bacteroides forsythus: a Comparative Study with Three Serotypes of Actinobacillus actinomycetemcomitans

    doi:

    Figure Lengend Snippet: Dose-dependent induction of apoptotic cell death by B. forsythus and A. actinomycetemcomitans strains. HL-60 cells were incubated in the presence of various concentrations of extracts, ranging from 0.1 to 50 μg of protein/ml for 24 or 48 h. All analyses were performed in duplicate experiments. The results shown are means and standard deviations.

    Article Snippet: For comparison with B. forsythus , the same analyses were applied to two strains with different serotypes of Actinobacillus actinomycetemcomitans , serotypes a (ATCC 43717) and c (ATCC 43719), in addition to previously reported apoptosis-inducing serotype b (ATCC 43718), which was used as a positive control.

    Techniques: Incubation

    Ultrastructural changes of HL-60 cells incubated with or without 50 μg of protein of bacterial sonic extracts per ml. Panels: A, B, and C, control HL-60 cells (treated with PBS, 10 μM adriamycin, and E. coli extract, respectively); D, E, and F, cells incubated for 48 h in the presence of the extracts from B. forsythus , A. actinomycetemcomitans serotype a, and A. actinomycetemcomitans serotype b, respectively; D-2, E-2, and F-2, photographs of corresponding samples at a higher magnification; G, cells incubated in the presence of the extract from A. actinomycetemcomitans serotype c, exhibiting cell surfaces similar to those of untreated HL-60 cells. Arrows indicate pores on cell membranes. Bar lengths and magnifications: A, 5.96 μm, ×5,000; B, 6.00 μm, ×4,500; C, 6.67 μm, ×5,000; D-1, 6.67 μm, ×4,500; D-2, 1.50 μm, ×20,000; E-1, 4.29 μm, ×7,000; E-2, 2.00 μm, ×15,000; F-1, 6.00 μm, ×5,000; F-2, 2.00 μm, ×15,000; G, 5.99 μm, ×5,000. Overall shrinkage was observed in cells treated with B. forsythus and A. actinomycetemcomitans serotype a extracts, and the calculated diameters were 5.8 and 5.6 μm, respectively, while that of control cells was approximately 7.5 μm.

    Journal: Infection and Immunity

    Article Title: Novel Apoptosis-Inducing Activity in Bacteroides forsythus: a Comparative Study with Three Serotypes of Actinobacillus actinomycetemcomitans

    doi:

    Figure Lengend Snippet: Ultrastructural changes of HL-60 cells incubated with or without 50 μg of protein of bacterial sonic extracts per ml. Panels: A, B, and C, control HL-60 cells (treated with PBS, 10 μM adriamycin, and E. coli extract, respectively); D, E, and F, cells incubated for 48 h in the presence of the extracts from B. forsythus , A. actinomycetemcomitans serotype a, and A. actinomycetemcomitans serotype b, respectively; D-2, E-2, and F-2, photographs of corresponding samples at a higher magnification; G, cells incubated in the presence of the extract from A. actinomycetemcomitans serotype c, exhibiting cell surfaces similar to those of untreated HL-60 cells. Arrows indicate pores on cell membranes. Bar lengths and magnifications: A, 5.96 μm, ×5,000; B, 6.00 μm, ×4,500; C, 6.67 μm, ×5,000; D-1, 6.67 μm, ×4,500; D-2, 1.50 μm, ×20,000; E-1, 4.29 μm, ×7,000; E-2, 2.00 μm, ×15,000; F-1, 6.00 μm, ×5,000; F-2, 2.00 μm, ×15,000; G, 5.99 μm, ×5,000. Overall shrinkage was observed in cells treated with B. forsythus and A. actinomycetemcomitans serotype a extracts, and the calculated diameters were 5.8 and 5.6 μm, respectively, while that of control cells was approximately 7.5 μm.

    Article Snippet: For comparison with B. forsythus , the same analyses were applied to two strains with different serotypes of Actinobacillus actinomycetemcomitans , serotypes a (ATCC 43717) and c (ATCC 43719), in addition to previously reported apoptosis-inducing serotype b (ATCC 43718), which was used as a positive control.

    Techniques: Incubation

    DNA ladder formation of HL-60 cells treated with bacterial extracts. HL-60 cells (5 × 10 5 ) was subjected to agarose gel electrophoresis and then stained with ethidium bromide. Lanes: 1, A. actinomycetemcomitans serotype a; 2, A. actinomycetemcomitans serotype b; 3, A. actinomycetemcomitans serotype c; 4, B. forsythus ; 5, E. coli ; 6, PBS. Nucleosomal DNA ladders characteristic of apoptotic cells were observed in the cells treated with B. forsythus , A. actinomycetemcomitans serotype a, and A. actinomycetemcomitans serotype b lysates.

    Journal: Infection and Immunity

    Article Title: Novel Apoptosis-Inducing Activity in Bacteroides forsythus: a Comparative Study with Three Serotypes of Actinobacillus actinomycetemcomitans

    doi:

    Figure Lengend Snippet: DNA ladder formation of HL-60 cells treated with bacterial extracts. HL-60 cells (5 × 10 5 ) was subjected to agarose gel electrophoresis and then stained with ethidium bromide. Lanes: 1, A. actinomycetemcomitans serotype a; 2, A. actinomycetemcomitans serotype b; 3, A. actinomycetemcomitans serotype c; 4, B. forsythus ; 5, E. coli ; 6, PBS. Nucleosomal DNA ladders characteristic of apoptotic cells were observed in the cells treated with B. forsythus , A. actinomycetemcomitans serotype a, and A. actinomycetemcomitans serotype b lysates.

    Article Snippet: For comparison with B. forsythus , the same analyses were applied to two strains with different serotypes of Actinobacillus actinomycetemcomitans , serotypes a (ATCC 43717) and c (ATCC 43719), in addition to previously reported apoptosis-inducing serotype b (ATCC 43718), which was used as a positive control.

    Techniques: Agarose Gel Electrophoresis, Staining

    Effects of 80 AU ml −1  of purified antimicrobial peptide AN5-1 produced by  P. alvei  AN5 on early exponential growth phase against target strains:  a E. coli  ATCC 29522,  b S. marcescens ,  c S. aureus  and  d B. cereus  ATCC 14579, in the absence ( filled circle ) and presence ( filled triangle ) of antimicrobial peptide AN5-1. The bacterial growth was measured by means of optical density at 600 nm (OD 600 )

    Journal: Journal of Industrial Microbiology & Biotechnology

    Article Title: Detection of secreted antimicrobial peptides isolated from cell-free culture supernatant of Paenibacillus alvei AN5

    doi: 10.1007/s10295-013-1259-5

    Figure Lengend Snippet: Effects of 80 AU ml −1 of purified antimicrobial peptide AN5-1 produced by P. alvei AN5 on early exponential growth phase against target strains: a E. coli ATCC 29522, b S. marcescens , c S. aureus and d B. cereus ATCC 14579, in the absence ( filled circle ) and presence ( filled triangle ) of antimicrobial peptide AN5-1. The bacterial growth was measured by means of optical density at 600 nm (OD 600 )

    Article Snippet: The inhibitory activities were measured at 1-h intervals after AN5-1 addition, where S. aureus and E. coli ATCC 29522 showed a relatively higher inhibitory effect (96.07 and 98.05 %) compared to S. marcescens (71.52 %) and B. cereus ATCC 14579 (72.77 %) (Table ).

    Techniques: Purification, Produced

    Tricine SDS-PAGE supplemented with glycerol for purified antimicrobial peptide AN5-1 produced by Paenibacillus alvei AN5. Lane 1 color marker ultra-low range (M.W. 1.06–26.6) KDa (Sigma, USA), lane 2 purified AN5-1 stained with Coomassie blue, lane 3 bacteriocin assay overlying on soft agar shows inhibitory activity of the active peptide against E. coli ATCC 29522

    Journal: Journal of Industrial Microbiology & Biotechnology

    Article Title: Detection of secreted antimicrobial peptides isolated from cell-free culture supernatant of Paenibacillus alvei AN5

    doi: 10.1007/s10295-013-1259-5

    Figure Lengend Snippet: Tricine SDS-PAGE supplemented with glycerol for purified antimicrobial peptide AN5-1 produced by Paenibacillus alvei AN5. Lane 1 color marker ultra-low range (M.W. 1.06–26.6) KDa (Sigma, USA), lane 2 purified AN5-1 stained with Coomassie blue, lane 3 bacteriocin assay overlying on soft agar shows inhibitory activity of the active peptide against E. coli ATCC 29522

    Article Snippet: The inhibitory activities were measured at 1-h intervals after AN5-1 addition, where S. aureus and E. coli ATCC 29522 showed a relatively higher inhibitory effect (96.07 and 98.05 %) compared to S. marcescens (71.52 %) and B. cereus ATCC 14579 (72.77 %) (Table ).

    Techniques: SDS Page, Purification, Produced, Chromosome Transmission Fidelity Colony Color Assay, Staining, Activity Assay

    Immunoblot analysis of ISTF production. (A) Whole fractions of A. actinomycetemcomitans standard strains and clinical isolates. Lane 1, molecular weight markers; lane 2, clinical isolate J1; lane 3, ATCC 29522; lane 4, ATCC 43717; lane 5, ATCC 43718; lane 6, ATCC 43719; lane 7, clinical isolate J2. (B) Each fraction of A. actinomycetemcomitans ATCC 29522 is shown. Lane 1, whole fraction; lane 2, insoluble fraction; lane 3, soluble fraction; lane 4, culture supernatant. In each lane, 10 μg of protein was loaded onto a 16.5% polyacrylamide gel. Equal protein loading and transfer were verified by Ponceau S staining of filters.

    Journal: Infection and Immunity

    Article Title: A Novel Factor Isolated from Actinobacillus actinomycetemcomitans Stimulates Mouse B Cells and Human Peripheral Blood Mononuclear Cells

    doi:

    Figure Lengend Snippet: Immunoblot analysis of ISTF production. (A) Whole fractions of A. actinomycetemcomitans standard strains and clinical isolates. Lane 1, molecular weight markers; lane 2, clinical isolate J1; lane 3, ATCC 29522; lane 4, ATCC 43717; lane 5, ATCC 43718; lane 6, ATCC 43719; lane 7, clinical isolate J2. (B) Each fraction of A. actinomycetemcomitans ATCC 29522 is shown. Lane 1, whole fraction; lane 2, insoluble fraction; lane 3, soluble fraction; lane 4, culture supernatant. In each lane, 10 μg of protein was loaded onto a 16.5% polyacrylamide gel. Equal protein loading and transfer were verified by Ponceau S staining of filters.

    Article Snippet: A. actinomycetemcomitans ATCC 29522 was cultured at 37°C in a CO2 -enriched atmosphere (10%) using brain heart infusion broth.

    Techniques: Molecular Weight, Staining

    q-PCR quantification of biofilms inoculated with one (A), two (B), and three (C) species grown on polystyrene pegs submerged in 25% saliva and incubated anaerobically for 12, 24, or 36 h. (A and C) Open bars, 12 h; black bars, 24 h; gray bars, 36 h. (B) Va, Veillonella sp. strain PK1910; Aa, A. actinomycetemcomitans JP2; Fn, F. nucleatum ATCC 10953. Statistically significant increases in bacterial growth on the pegs are indicated in the lower portion of each panel; for example, “Va 12 h - 24 h” indicates that there was significant growth of Veillonella sp. strain PK1910 between 12 h and 24 h in a peg biofilm inoculated with two species.

    Journal: Infection and Immunity

    Article Title: Aggregatibacter actinomycetemcomitans Builds Mutualistic Biofilm Communities with Fusobacterium nucleatum and Veillonella Species in Saliva

    doi: 10.1128/IAI.00345-09

    Figure Lengend Snippet: q-PCR quantification of biofilms inoculated with one (A), two (B), and three (C) species grown on polystyrene pegs submerged in 25% saliva and incubated anaerobically for 12, 24, or 36 h. (A and C) Open bars, 12 h; black bars, 24 h; gray bars, 36 h. (B) Va, Veillonella sp. strain PK1910; Aa, A. actinomycetemcomitans JP2; Fn, F. nucleatum ATCC 10953. Statistically significant increases in bacterial growth on the pegs are indicated in the lower portion of each panel; for example, “Va 12 h - 24 h” indicates that there was significant growth of Veillonella sp. strain PK1910 between 12 h and 24 h in a peg biofilm inoculated with two species.

    Article Snippet: Veillonella sp. strain PK1910, Aggregatibacter actinomycetemcomitans JP2, and Fusobacterium nucleatum ATCC 10953 were unable to grow as single species in flow cells.

    Techniques: Polymerase Chain Reaction, Incubation

    Phase-contrast micrographs of one-species suspensions (left panels), two-species coaggregates (right panels), and three-species coaggregates (bottom panel) in microtiter wells immediately before immersion of polystyrene pegs. Va, Veillonella sp. strain PK1910; Aa, A. actinomycetemcomitans JP2; Fn, F. nucleatum ATCC 10953. Bars, 5 μm.

    Journal: Infection and Immunity

    Article Title: Aggregatibacter actinomycetemcomitans Builds Mutualistic Biofilm Communities with Fusobacterium nucleatum and Veillonella Species in Saliva

    doi: 10.1128/IAI.00345-09

    Figure Lengend Snippet: Phase-contrast micrographs of one-species suspensions (left panels), two-species coaggregates (right panels), and three-species coaggregates (bottom panel) in microtiter wells immediately before immersion of polystyrene pegs. Va, Veillonella sp. strain PK1910; Aa, A. actinomycetemcomitans JP2; Fn, F. nucleatum ATCC 10953. Bars, 5 μm.

    Article Snippet: Veillonella sp. strain PK1910, Aggregatibacter actinomycetemcomitans JP2, and Fusobacterium nucleatum ATCC 10953 were unable to grow as single species in flow cells.

    Techniques:

    Confocal micrographs of biofilms formed in flow cells inoculated with one species after 4 h (left panels) and 18 h (right panels) of growth on 25% saliva. The organisms used were Veillonella sp. strain PK1910, A. actinomycetemcomitans JP2, and F. nucleatum ATCC 10953. The colonization (4 h) and growth (18 h) of each species was monitored. Bacterial cells were stained with species-specific fluorophore-conjugated immunoglobulin G.

    Journal: Infection and Immunity

    Article Title: Aggregatibacter actinomycetemcomitans Builds Mutualistic Biofilm Communities with Fusobacterium nucleatum and Veillonella Species in Saliva

    doi: 10.1128/IAI.00345-09

    Figure Lengend Snippet: Confocal micrographs of biofilms formed in flow cells inoculated with one species after 4 h (left panels) and 18 h (right panels) of growth on 25% saliva. The organisms used were Veillonella sp. strain PK1910, A. actinomycetemcomitans JP2, and F. nucleatum ATCC 10953. The colonization (4 h) and growth (18 h) of each species was monitored. Bacterial cells were stained with species-specific fluorophore-conjugated immunoglobulin G.

    Article Snippet: Veillonella sp. strain PK1910, Aggregatibacter actinomycetemcomitans JP2, and Fusobacterium nucleatum ATCC 10953 were unable to grow as single species in flow cells.

    Techniques: Flow Cytometry, Staining

    Representative confocal micrographs of 4-h (left panels) and 18-h (right panels) biofilms showing mutualistic growth in flow cells inoculated with two species. The organisms used were Veillonella sp. strain PK1910, A. actinomycetemcomitans JP2, and F. nucleatum ATCC 10953. Bacterial cells were stained with species-specific fluorophore-conjugated immunoglobulin G (blue, Veillonella sp. strain PK1910; green, A. actinomycetemcomitans JP2; red, F. nucleatum ATCC 10953), and cell-cell contact is evident.

    Journal: Infection and Immunity

    Article Title: Aggregatibacter actinomycetemcomitans Builds Mutualistic Biofilm Communities with Fusobacterium nucleatum and Veillonella Species in Saliva

    doi: 10.1128/IAI.00345-09

    Figure Lengend Snippet: Representative confocal micrographs of 4-h (left panels) and 18-h (right panels) biofilms showing mutualistic growth in flow cells inoculated with two species. The organisms used were Veillonella sp. strain PK1910, A. actinomycetemcomitans JP2, and F. nucleatum ATCC 10953. Bacterial cells were stained with species-specific fluorophore-conjugated immunoglobulin G (blue, Veillonella sp. strain PK1910; green, A. actinomycetemcomitans JP2; red, F. nucleatum ATCC 10953), and cell-cell contact is evident.

    Article Snippet: Veillonella sp. strain PK1910, Aggregatibacter actinomycetemcomitans JP2, and Fusobacterium nucleatum ATCC 10953 were unable to grow as single species in flow cells.

    Techniques: Flow Cytometry, Staining

    Representative confocal micrographs of flow cells inoculated with three species. The communities at 4 h and 18 h show the intimate interaction of Veillonella sp. strain PK1910 (blue) with A. actinomycetemcomitans JP2 (green) and F. nucleatum ATCC 10953 (red) that formed mutualistic multispecies communities.

    Journal: Infection and Immunity

    Article Title: Aggregatibacter actinomycetemcomitans Builds Mutualistic Biofilm Communities with Fusobacterium nucleatum and Veillonella Species in Saliva

    doi: 10.1128/IAI.00345-09

    Figure Lengend Snippet: Representative confocal micrographs of flow cells inoculated with three species. The communities at 4 h and 18 h show the intimate interaction of Veillonella sp. strain PK1910 (blue) with A. actinomycetemcomitans JP2 (green) and F. nucleatum ATCC 10953 (red) that formed mutualistic multispecies communities.

    Article Snippet: Veillonella sp. strain PK1910, Aggregatibacter actinomycetemcomitans JP2, and Fusobacterium nucleatum ATCC 10953 were unable to grow as single species in flow cells.

    Techniques: Flow Cytometry

    Twelve representative A. actinomycetemcomitans DNA probe profiles from Southern blot analysis. A. actinomycetemcomitans genomic DNA was digested to completion with Eco RI and hybridized with digoxigenin-labeled, 5.2 kb cloned DNA from strain Y4. The probe was detected with alkaline phosphatase-conjugated anti-digoxigenin antibody and enzyme substrate. Lane 1, ATCC 29522; lane 2, strain 641; lane 3. NCTC 9710 T ; lane 4, strain 79; lane 5, strain 681; lane 6, strain 631; lane 7, strain 606; lane 8. strain 474; lane 9, strain 266; lane 10, ATCC 29524; lane 11, strain 283; lane 12, strain 29.

    Journal: Oral microbiology and immunology

    Article Title: Evaluating two methods for fingerprinting genomes of Actinobacillus actinomycetemcomitans

    doi:

    Figure Lengend Snippet: Twelve representative A. actinomycetemcomitans DNA probe profiles from Southern blot analysis. A. actinomycetemcomitans genomic DNA was digested to completion with Eco RI and hybridized with digoxigenin-labeled, 5.2 kb cloned DNA from strain Y4. The probe was detected with alkaline phosphatase-conjugated anti-digoxigenin antibody and enzyme substrate. Lane 1, ATCC 29522; lane 2, strain 641; lane 3. NCTC 9710 T ; lane 4, strain 79; lane 5, strain 681; lane 6, strain 631; lane 7, strain 606; lane 8. strain 474; lane 9, strain 266; lane 10, ATCC 29524; lane 11, strain 283; lane 12, strain 29.

    Article Snippet: A. actinomycetemcomitans strains ATCC 29522, ATCC 29523 and ATCC 29524 were obtained from the American Type Culture Collection, Rockville, MD, strain NCTC 9710T from the National Collection of Type Cultures, London, UK, strain Y4 from S.S. Socransky, Forsyth Dental Center, Boston, MA and strain JP2 from N. S. Taichman, University of Pennsylvania School of Dental Medicine, Philadelphia, PA.

    Techniques: Southern Blot, Labeling, Clone Assay

    Inhibition (a) and eracdication (b) of biofilm formation of S. mutans ATCC 25175 (□) and A. actinomycetemcomitans ATCC 33384 (■) by P. betle leaves extract at various concentrations, 0.1% (w/v) CHX was used as control. Error bars indicate standard deviations; n = 6.

    Journal: Journal of Traditional and Complementary Medicine

    Article Title: Screening for antibacterial and antibiofilm activity in Thai medicinal plant extracts against oral microorganisms

    doi: 10.1016/j.jtcme.2016.06.007

    Figure Lengend Snippet: Inhibition (a) and eracdication (b) of biofilm formation of S. mutans ATCC 25175 (□) and A. actinomycetemcomitans ATCC 33384 (■) by P. betle leaves extract at various concentrations, 0.1% (w/v) CHX was used as control. Error bars indicate standard deviations; n = 6.

    Article Snippet: 3.3 Effect of P. betle extract on inhibition and eradication biofilm formation The inhibition and eradication of the biofilm formation of P. betle extract against S. mutans ATCC 25175 and A. actinomycetemcomitans ATCC 33384 is demonstrated in a and b, respectively.

    Techniques: Inhibition

    Time-kill curves of S. mutans ATCC 25175 (a) and A. actinomycetemcomitans ATCC 33384 (b) at different concentrations of P. betle extract: 0 × MIC (▵), 1 × MIC (▾), 2 × MIC (○), and 4 × MIC (●); 0.1% (w/v) CHX (■); CFU, Colony Forming Units.

    Journal: Journal of Traditional and Complementary Medicine

    Article Title: Screening for antibacterial and antibiofilm activity in Thai medicinal plant extracts against oral microorganisms

    doi: 10.1016/j.jtcme.2016.06.007

    Figure Lengend Snippet: Time-kill curves of S. mutans ATCC 25175 (a) and A. actinomycetemcomitans ATCC 33384 (b) at different concentrations of P. betle extract: 0 × MIC (▵), 1 × MIC (▾), 2 × MIC (○), and 4 × MIC (●); 0.1% (w/v) CHX (■); CFU, Colony Forming Units.

    Article Snippet: 3.3 Effect of P. betle extract on inhibition and eradication biofilm formation The inhibition and eradication of the biofilm formation of P. betle extract against S. mutans ATCC 25175 and A. actinomycetemcomitans ATCC 33384 is demonstrated in a and b, respectively.

    Techniques:

    Adhesion of wild-type A. actinomycetemcomitans and Aae mutant strains to KB monolayers. SUNY 465 and ATCC 29523 strains were used. Experiments were performed with quadruplicate wells for each strain. Results shown are from a typical experiment; bars represent the standard deviation of the replicates.

    Journal: Infection and Immunity

    Article Title: Aae, an Autotransporter Involved in Adhesion of Actinobacillus actinomycetemcomitans to Epithelial Cells

    doi: 10.1128/IAI.71.5.2384-2393.2003

    Figure Lengend Snippet: Adhesion of wild-type A. actinomycetemcomitans and Aae mutant strains to KB monolayers. SUNY 465 and ATCC 29523 strains were used. Experiments were performed with quadruplicate wells for each strain. Results shown are from a typical experiment; bars represent the standard deviation of the replicates.

    Article Snippet: Human milk whey had similar effects on Aae from A. actinomycetemcomitans ATCC 29523 and its binding to epithelial cells; however, there was little effect on the binding of SUNY 465.

    Techniques: Mutagenesis, Standard Deviation