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  • 95
    Chem Impex International eplerenone
    Eplerenone, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eplerenone/product/Chem Impex International
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    92
    Proteintech xdh antibody
    Xdh Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xdh antibody/product/Proteintech
    Average 92 stars, based on 1 article reviews
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    xdh antibody - by Bioz Stars, 2024-07
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    92
    Proteintech xanthine oxidase xo
    Xanthine Oxidase Xo, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Rockland Immunochemicals rabbit anti xor rabbit antibody
    ( A ) <t>IF</t> <t>staining</t> of <t>XOR.</t> HeLa (a) and HT29 (b) cells were analyzed for XOR expression by IF staining with an anti-XOR antibody. (c) The specificity of the antibody on HeLa cells was confirmed by pre-mixing anti-XOR antibody (1:50 in 200 μl) with purified XOR (2 μg/200 μl). Normal rabbit IgG was included as a negative control (d). ( B – D ) Genotoxic stress does not alter XOR expression. HeLa and HT29 cells were left untreated or treated with 5-FU (10 μM), gemcitabine (2 μM) or radiation (40 Gy) for 24 hr (B) or treated with 5-FU (10 μM) or gemcitabine (2 μM) in HeLa (C) and HT29 cells (D) for the indicated time. Cell lysates were prepared in NP-40 lysis buffer and analyzed for XOR expression by Western blot with a rabbit anti-XOR antiserum.
    Rabbit Anti Xor Rabbit Antibody, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Proteintech anti xod
    Downregulation of the components of Fe-S cluster biogenesis machinery in Irp1 and 2 deficient fibroblasts is specifically associated with impaired electron transport chain (ETC). ( a ) A representative graph of in-gel assays of mitochondrial (m-Aco, encoded by Aco2) and cytosolic (c-Aco, encoded by Irp1) aconitases in Irp deficient MEF cells (upper panel). The protein levels of Aco2, Irp1, and cytosolic Fe-S containing enzyme xanthine oxidative <t>(Xod)</t> were detected with western blotting. ( b ) The activities of Xod, m-Aco, and c-Aco were quantified. ( c ) Activities of ETC complexes were determined in Irp deficient MEFs. ( d ) Western blot analysis of mitochondrial proteins including Ndufs3 (a subunit of <t>CI),</t> <t>SdhA</t> and SdhB (subunits of CII), Uqcrc1 and Uqcrfs1 (subunits of CIII), Fech (matrix protein), CytC (intermembrane space protein), Vdac (outer membrane protein), and citrate synthase (Cs, matrix non-Fe-S protein). A representative image set is presented. ( e ) Activities of citrate synthase, which is a mitochondrial non-Fe-S enzyme, were determined. CI/CII/CIII/CIV: Complex I/II/III/IV. Values represent mean ± SEM (n = 10 for ( e ), n = 3, each duplicates for ( b ) and ( c )). A one-way ANOVA was performed. * p < 0.05, ** p < 0.01, *** p < 0.001 compared with WT.
    Anti Xod, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    ( A ) IF staining of XOR. HeLa (a) and HT29 (b) cells were analyzed for XOR expression by IF staining with an anti-XOR antibody. (c) The specificity of the antibody on HeLa cells was confirmed by pre-mixing anti-XOR antibody (1:50 in 200 μl) with purified XOR (2 μg/200 μl). Normal rabbit IgG was included as a negative control (d). ( B – D ) Genotoxic stress does not alter XOR expression. HeLa and HT29 cells were left untreated or treated with 5-FU (10 μM), gemcitabine (2 μM) or radiation (40 Gy) for 24 hr (B) or treated with 5-FU (10 μM) or gemcitabine (2 μM) in HeLa (C) and HT29 cells (D) for the indicated time. Cell lysates were prepared in NP-40 lysis buffer and analyzed for XOR expression by Western blot with a rabbit anti-XOR antiserum.

    Journal: Oncotarget

    Article Title: Xanthine oxidoreductase is required for genotoxic stress-induced NKG2D ligand expression and gemcitabine-mediated antitumor activity

    doi: 10.18632/oncotarget.11042

    Figure Lengend Snippet: ( A ) IF staining of XOR. HeLa (a) and HT29 (b) cells were analyzed for XOR expression by IF staining with an anti-XOR antibody. (c) The specificity of the antibody on HeLa cells was confirmed by pre-mixing anti-XOR antibody (1:50 in 200 μl) with purified XOR (2 μg/200 μl). Normal rabbit IgG was included as a negative control (d). ( B – D ) Genotoxic stress does not alter XOR expression. HeLa and HT29 cells were left untreated or treated with 5-FU (10 μM), gemcitabine (2 μM) or radiation (40 Gy) for 24 hr (B) or treated with 5-FU (10 μM) or gemcitabine (2 μM) in HeLa (C) and HT29 cells (D) for the indicated time. Cell lysates were prepared in NP-40 lysis buffer and analyzed for XOR expression by Western blot with a rabbit anti-XOR antiserum.

    Article Snippet: XOR expression was detected by IF staining with a rabbit anti-XOR rabbit antibody (Rockland Immunologicals, Inc., Bertsville, PA) (1:100) and examined under a fluorescence microscope.

    Techniques: Staining, Expressing, Purification, Negative Control, Lysis, Western Blot

    ( A ) Immunofluorescence (IF) staining of XOR. HeLa cells stably transfected with control miRNA (Ctr-miRNA) or XOR-miRNA were analyzed for XOR expression by IF staining with an anti-XOR antibody as described in Figure . Nuclei was stained with DAPI. ( B and C ) XOR knockdown blocks uric acid production and MICA/B expression. Ctr-miRNA and XOR-miRNA-transfected cells were exposed to 5-FU (10 μM), gemcitabine (2 μM), or radiation (40 Gy). Cells were harvested at 24 hr and analyzed for intracellular uric acid levels using a uric acid assay kit (B) or for MICA/B expression by FACS (C). Black line, isotype control; Green line, MICA/B. Inset in (B): XOR expression analyzed by Western blot. Actin was included as a loading control. ( D ) Western blot analysis of XOR expression in Ctr-miRNA- and XOR-miRNA-transfected RCAS-Neu cells. ( E ) XOR knockdown blocks genotoxic stress-induced Rae I expression. Ctr-miRNA- and XOR-miRNA-transfected RCAS-Neucells were exposed to 5-FU (10 μM) or gemcitabine (2 μM) and analyzed for Rae I expression 16 hr later by FACS analysis.

    Journal: Oncotarget

    Article Title: Xanthine oxidoreductase is required for genotoxic stress-induced NKG2D ligand expression and gemcitabine-mediated antitumor activity

    doi: 10.18632/oncotarget.11042

    Figure Lengend Snippet: ( A ) Immunofluorescence (IF) staining of XOR. HeLa cells stably transfected with control miRNA (Ctr-miRNA) or XOR-miRNA were analyzed for XOR expression by IF staining with an anti-XOR antibody as described in Figure . Nuclei was stained with DAPI. ( B and C ) XOR knockdown blocks uric acid production and MICA/B expression. Ctr-miRNA and XOR-miRNA-transfected cells were exposed to 5-FU (10 μM), gemcitabine (2 μM), or radiation (40 Gy). Cells were harvested at 24 hr and analyzed for intracellular uric acid levels using a uric acid assay kit (B) or for MICA/B expression by FACS (C). Black line, isotype control; Green line, MICA/B. Inset in (B): XOR expression analyzed by Western blot. Actin was included as a loading control. ( D ) Western blot analysis of XOR expression in Ctr-miRNA- and XOR-miRNA-transfected RCAS-Neu cells. ( E ) XOR knockdown blocks genotoxic stress-induced Rae I expression. Ctr-miRNA- and XOR-miRNA-transfected RCAS-Neucells were exposed to 5-FU (10 μM) or gemcitabine (2 μM) and analyzed for Rae I expression 16 hr later by FACS analysis.

    Article Snippet: XOR expression was detected by IF staining with a rabbit anti-XOR rabbit antibody (Rockland Immunologicals, Inc., Bertsville, PA) (1:100) and examined under a fluorescence microscope.

    Techniques: Immunofluorescence, Staining, Stable Transfection, Transfection, Expressing, Uric Acid Assay, Western Blot

    Downregulation of the components of Fe-S cluster biogenesis machinery in Irp1 and 2 deficient fibroblasts is specifically associated with impaired electron transport chain (ETC). ( a ) A representative graph of in-gel assays of mitochondrial (m-Aco, encoded by Aco2) and cytosolic (c-Aco, encoded by Irp1) aconitases in Irp deficient MEF cells (upper panel). The protein levels of Aco2, Irp1, and cytosolic Fe-S containing enzyme xanthine oxidative (Xod) were detected with western blotting. ( b ) The activities of Xod, m-Aco, and c-Aco were quantified. ( c ) Activities of ETC complexes were determined in Irp deficient MEFs. ( d ) Western blot analysis of mitochondrial proteins including Ndufs3 (a subunit of CI), SdhA and SdhB (subunits of CII), Uqcrc1 and Uqcrfs1 (subunits of CIII), Fech (matrix protein), CytC (intermembrane space protein), Vdac (outer membrane protein), and citrate synthase (Cs, matrix non-Fe-S protein). A representative image set is presented. ( e ) Activities of citrate synthase, which is a mitochondrial non-Fe-S enzyme, were determined. CI/CII/CIII/CIV: Complex I/II/III/IV. Values represent mean ± SEM (n = 10 for ( e ), n = 3, each duplicates for ( b ) and ( c )). A one-way ANOVA was performed. * p < 0.05, ** p < 0.01, *** p < 0.001 compared with WT.

    Journal: Scientific Reports

    Article Title: Iron regulatory protein deficiency compromises mitochondrial function in murine embryonic fibroblasts

    doi: 10.1038/s41598-018-23175-y

    Figure Lengend Snippet: Downregulation of the components of Fe-S cluster biogenesis machinery in Irp1 and 2 deficient fibroblasts is specifically associated with impaired electron transport chain (ETC). ( a ) A representative graph of in-gel assays of mitochondrial (m-Aco, encoded by Aco2) and cytosolic (c-Aco, encoded by Irp1) aconitases in Irp deficient MEF cells (upper panel). The protein levels of Aco2, Irp1, and cytosolic Fe-S containing enzyme xanthine oxidative (Xod) were detected with western blotting. ( b ) The activities of Xod, m-Aco, and c-Aco were quantified. ( c ) Activities of ETC complexes were determined in Irp deficient MEFs. ( d ) Western blot analysis of mitochondrial proteins including Ndufs3 (a subunit of CI), SdhA and SdhB (subunits of CII), Uqcrc1 and Uqcrfs1 (subunits of CIII), Fech (matrix protein), CytC (intermembrane space protein), Vdac (outer membrane protein), and citrate synthase (Cs, matrix non-Fe-S protein). A representative image set is presented. ( e ) Activities of citrate synthase, which is a mitochondrial non-Fe-S enzyme, were determined. CI/CII/CIII/CIV: Complex I/II/III/IV. Values represent mean ± SEM (n = 10 for ( e ), n = 3, each duplicates for ( b ) and ( c )). A one-way ANOVA was performed. * p < 0.05, ** p < 0.01, *** p < 0.001 compared with WT.

    Article Snippet: The information for primary antibodies is as follows: anti-ferritin (cat# 69090), SdhA (cat# 137040), and SdhB (cat# 178423) from Abcam (Cambridge, MA, USA), anti-Xod (cat# 55156-1-AP), citrate synthase (cat# 16131-1-AP), aconitase 2 (Aco2) (cat# 11134-1-AP), Ndufs1 (cat# 12444-1-AP), Ndufs3 (cat# 15066-1-AP), Uqcrc1 (cat# 21705-1-AP), and Uqcrfs1 (cat# 1843-1-AP) from Proteintech Group Inc. (Chicago, IN, USA), anti-Actin (cat# BM0627) from Boster (Wuhan, China), anti-Tubulin (cat# T0198) from Sigma-Aldrich (St. Louis, MO, USA), anti-TfR1 antibody (cat# 136800) from Zymed (San Francisco, CA, USA), anti-IscU, Fech, Irp1, and Irp2, anti-Fxn (self-made) , anti-CytC (cat# 1896-1) from Epitmics (Burlingame, CA, USA), anti-Vdac (cat# 4661S) from Cell Signaling Technology Inc (Shanghai, China).

    Techniques: Western Blot