Western Blot Antibodies Search Results


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    Cell Signaling Technology Inc akt
    Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore monoclonal anti flag m2 antibody
    Characterization of 3xFLAG-Pof8. a Western blot using <t>α-FLAG</t> <t>antibody</t> in strains containing untagged Pof8 or 3xFLAG-Pof8 with two independent isolates per strain in an Lsm4-cMyc background. A non-specific band recognized by α-FLAG in S. pombe extracts even in the absence of an epitope-tagged protein was used as a loading control (LC). b Telomeric Southern blot from strains containing untagged Pof8 or 3xFLAG-Pof8 on a plasmid under the control of its endogenous promoter with two independent isolates per strain in an Lsm4-cMyc background. A rad16 + probe was used as the LC. c UV crosslinking and immunoprecipitation of in vitro transcribed TER1 probe incubated in extracts containing epitope-tagged Lsm4 or Pof8
    Monoclonal Anti Flag M2 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc cleaved caspase 3
    REV-ERB agonist SR9011 inhibit autophagy a–b, SR90011 treatment reduces the number of autophagosomes both in MCF7 and T47D, n= biological independent samples MCF7 20µM 24h n=9 (mock), n=4 (SR9011) ** P =0.0056, T47D 48h 20µM n=5 (mock), n=4 (SR9011) ** P =0.0079; c–d, SR9011 induces accumulation of p62 as shown by immunofluorescence both in MCF7 and T47D n= biological independent samples 48h MCF7 p62 n=3 (mock), n=4 (SR9011) * P =0.0286; 48h T47D n=5 (mock, SR9011) ** P =0.004; e, Accumulation of p62 is confirmed by immunoblot (48h, 20µM A375); f–g, Inhibition of autophagy precedes apoptosis induction as shown by immunofluorescence of p62, cleaved <t>Caspase</t> 3 and TUNEL assay (n= biological independent samples n=4 mock 48h, n=5 SR9011 p62, n=3 48h SR9011; n=6 (mock, SR9011 72h p62), n=10 (mock 72h), n=8 (SR9011 72h) A375 20µM, Cl. Casp. 3 48h * P =0.0286; Cl. Casp. 3 72h **** P
    Cleaved Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc caspase 3
    Evaluation of V-9302  in vivo  in HCC1806 cell line xenograft-bearing mice ( A ) Tumor volumetric analysis of mice treated with vehicle or V-9302 (75 mg/kg per day) for 10 days.  n  = 5 mice per group. Treatment started 9 days post injection. P value at day 10 determined by Student’s  t  test. ( B ) Immunofluorescence analysis of tumor tissues harvested from vehicle- or V-9302-treated mice; LC3B and pAKT (Ser473) shown (pink). CD-31 positive vessels shown in green and nuclei in blue (DAPI). 20× magnification. P values determined by Student’s  t  test. ( C ) Effects of V-9302 treatment on pS6-positive cells and caspase 3-positive cells by immunohistochemistry. Quantitative analysis consisted of the mean counts of at least three representative fields from three vehicle and three V-9302-treated mice. For box plots, center line is plotted at the median; the box spans from the first quartile to the third quartile; whiskers represent min to max. Error bars represent ± std. dev.
    Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/caspase 3/product/Cell Signaling Technology Inc
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    Monoclonal antibody to GFP Western Blot Kit Conjugation Note Unconjugated Application Note WB
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    Polyclonal antibody to RFP Western Blot Kit Conjugation Note Unconjugated Application Note WB
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    Characterization of 3xFLAG-Pof8. a Western blot using α-FLAG antibody in strains containing untagged Pof8 or 3xFLAG-Pof8 with two independent isolates per strain in an Lsm4-cMyc background. A non-specific band recognized by α-FLAG in S. pombe extracts even in the absence of an epitope-tagged protein was used as a loading control (LC). b Telomeric Southern blot from strains containing untagged Pof8 or 3xFLAG-Pof8 on a plasmid under the control of its endogenous promoter with two independent isolates per strain in an Lsm4-cMyc background. A rad16 + probe was used as the LC. c UV crosslinking and immunoprecipitation of in vitro transcribed TER1 probe incubated in extracts containing epitope-tagged Lsm4 or Pof8

    Journal: Nature Communications

    Article Title: Pof8 is a La-related protein and a constitutive component of telomerase in fission yeast

    doi: 10.1038/s41467-017-02284-8

    Figure Lengend Snippet: Characterization of 3xFLAG-Pof8. a Western blot using α-FLAG antibody in strains containing untagged Pof8 or 3xFLAG-Pof8 with two independent isolates per strain in an Lsm4-cMyc background. A non-specific band recognized by α-FLAG in S. pombe extracts even in the absence of an epitope-tagged protein was used as a loading control (LC). b Telomeric Southern blot from strains containing untagged Pof8 or 3xFLAG-Pof8 on a plasmid under the control of its endogenous promoter with two independent isolates per strain in an Lsm4-cMyc background. A rad16 + probe was used as the LC. c UV crosslinking and immunoprecipitation of in vitro transcribed TER1 probe incubated in extracts containing epitope-tagged Lsm4 or Pof8

    Article Snippet: Magnetic dynabeads protein G (30 mg ml−1 ; Invitrogen) was coated with anti-c-Myc 9E10 or anti-FLAG M2 (10 µg per 50 µl of bead suspension; Sigma-Aldrich, M4439 and F3165) by incubation for 30 min at room temperature in 200 µl of 1× PBS + 0.1% (v/v) Tween-20.

    Techniques: Western Blot, Southern Blot, Plasmid Preparation, Immunoprecipitation, In Vitro, Incubation

    Pof8 is not a general loading factor for Lsm2-8. a Northern blot for U6 snRNA from input (10%), supernatant (S/N, 10%), and immunoprecipitates (IP, 100%) with anti-FLAG from extracts of cells expressing untagged or FLAG epitope-tagged Pof8. b Scatter plot relating changes in gene expression upon deletion of pof8 to enrichment of RNAs in Lsm8 immunoprecipitate. Gene expression in log 2 average RPKM is plotted on the x -axis and the log 2 fold change between pof8Δ and wildtype on the y -axis. Differentially expressed genes with an absolute log 2 fold change of ≥1 and an adjusted p -value

    Journal: Nature Communications

    Article Title: Pof8 is a La-related protein and a constitutive component of telomerase in fission yeast

    doi: 10.1038/s41467-017-02284-8

    Figure Lengend Snippet: Pof8 is not a general loading factor for Lsm2-8. a Northern blot for U6 snRNA from input (10%), supernatant (S/N, 10%), and immunoprecipitates (IP, 100%) with anti-FLAG from extracts of cells expressing untagged or FLAG epitope-tagged Pof8. b Scatter plot relating changes in gene expression upon deletion of pof8 to enrichment of RNAs in Lsm8 immunoprecipitate. Gene expression in log 2 average RPKM is plotted on the x -axis and the log 2 fold change between pof8Δ and wildtype on the y -axis. Differentially expressed genes with an absolute log 2 fold change of ≥1 and an adjusted p -value

    Article Snippet: Magnetic dynabeads protein G (30 mg ml−1 ; Invitrogen) was coated with anti-c-Myc 9E10 or anti-FLAG M2 (10 µg per 50 µl of bead suspension; Sigma-Aldrich, M4439 and F3165) by incubation for 30 min at room temperature in 200 µl of 1× PBS + 0.1% (v/v) Tween-20.

    Techniques: Northern Blot, Expressing, FLAG-tag

    REV-ERB agonist SR9011 inhibit autophagy a–b, SR90011 treatment reduces the number of autophagosomes both in MCF7 and T47D, n= biological independent samples MCF7 20µM 24h n=9 (mock), n=4 (SR9011) ** P =0.0056, T47D 48h 20µM n=5 (mock), n=4 (SR9011) ** P =0.0079; c–d, SR9011 induces accumulation of p62 as shown by immunofluorescence both in MCF7 and T47D n= biological independent samples 48h MCF7 p62 n=3 (mock), n=4 (SR9011) * P =0.0286; 48h T47D n=5 (mock, SR9011) ** P =0.004; e, Accumulation of p62 is confirmed by immunoblot (48h, 20µM A375); f–g, Inhibition of autophagy precedes apoptosis induction as shown by immunofluorescence of p62, cleaved Caspase 3 and TUNEL assay (n= biological independent samples n=4 mock 48h, n=5 SR9011 p62, n=3 48h SR9011; n=6 (mock, SR9011 72h p62), n=10 (mock 72h), n=8 (SR9011 72h) A375 20µM, Cl. Casp. 3 48h * P =0.0286; Cl. Casp. 3 72h **** P

    Journal: Nature

    Article Title: Pharmacological activation of REV-ERBs is lethal in cancer and oncogene induced senescence

    doi: 10.1038/nature25170

    Figure Lengend Snippet: REV-ERB agonist SR9011 inhibit autophagy a–b, SR90011 treatment reduces the number of autophagosomes both in MCF7 and T47D, n= biological independent samples MCF7 20µM 24h n=9 (mock), n=4 (SR9011) ** P =0.0056, T47D 48h 20µM n=5 (mock), n=4 (SR9011) ** P =0.0079; c–d, SR9011 induces accumulation of p62 as shown by immunofluorescence both in MCF7 and T47D n= biological independent samples 48h MCF7 p62 n=3 (mock), n=4 (SR9011) * P =0.0286; 48h T47D n=5 (mock, SR9011) ** P =0.004; e, Accumulation of p62 is confirmed by immunoblot (48h, 20µM A375); f–g, Inhibition of autophagy precedes apoptosis induction as shown by immunofluorescence of p62, cleaved Caspase 3 and TUNEL assay (n= biological independent samples n=4 mock 48h, n=5 SR9011 p62, n=3 48h SR9011; n=6 (mock, SR9011 72h p62), n=10 (mock 72h), n=8 (SR9011 72h) A375 20µM, Cl. Casp. 3 48h * P =0.0286; Cl. Casp. 3 72h **** P

    Article Snippet: Apoptosis was evaluated by immunostaining of cleaved caspase 3 (Cell signaling #9664 1:200) and by TUNEL assay using In Situ Cell Death Detection Kit, Fluoresceine or TMR red (Roche).

    Techniques: Immunofluorescence, Inhibition, TUNEL Assay

    SR9009 and SR9011 treatment evokes an apoptotic response and induces inhibition of autophagy in OIS cells a, Proliferation assay shows that REV-ERBs agonists impair viability of OIS cells (6 days, 20µM). b–c, Immunofluorescence assay for cleaved Caspase 3 and TUNEL assay shows apoptosis induction specifically in OIS (n=biological independent samples, n=7 mock, n=9 SR9009, n=14 SR9011, 72h, 20µM; one-way ANOVA, Cl. Casp 3 **** P

    Journal: Nature

    Article Title: Pharmacological activation of REV-ERBs is lethal in cancer and oncogene induced senescence

    doi: 10.1038/nature25170

    Figure Lengend Snippet: SR9009 and SR9011 treatment evokes an apoptotic response and induces inhibition of autophagy in OIS cells a, Proliferation assay shows that REV-ERBs agonists impair viability of OIS cells (6 days, 20µM). b–c, Immunofluorescence assay for cleaved Caspase 3 and TUNEL assay shows apoptosis induction specifically in OIS (n=biological independent samples, n=7 mock, n=9 SR9009, n=14 SR9011, 72h, 20µM; one-way ANOVA, Cl. Casp 3 **** P

    Article Snippet: Apoptosis was evaluated by immunostaining of cleaved caspase 3 (Cell signaling #9664 1:200) and by TUNEL assay using In Situ Cell Death Detection Kit, Fluoresceine or TMR red (Roche).

    Techniques: Inhibition, Proliferation Assay, Immunofluorescence, TUNEL Assay

    REV-ERBs agonist SR9009 inhibits autophagy a–b , SR9009 treatment reduces the number of autophagosomes, as shown by immunofluorescence of LC3B, (n=biological independent samples MCF7 (n=6 mock), (n=5 SR9009) and T47D (n=5 mock) (n=4 SR9009) Mann–Whitney test one-tailed MCF7 20µM 24h * P =0.0152, T47D 20µM 48h ** P =0.0079; c–d SR9009 induces accumulation of p62 as shown by immunofluorescence; n= biological independent samples MCF7 (n=3 mock), (n=8 SR9009) and T47D (n=5 mock), (n=4 SR9009) Mann–Whitney test one-tailed 48h MCF7 p62 ** P =0.0061; 48h T47D ** P =0.0079; e, Inhibition of autophagy is confirmed by the immunoblot for p62 (20µM 48h, A375); f–g, Inhibition of autophagy precedes apoptosis induction as shown by immunofluorescence of p62, cleaved Caspase 3 and TUNEL assay; n=biological independent samples, Mann–Whitney test one-tailed, A375 20µM Cl. Casp. 3 48h (n=3) * P =0.0179; Cl. Casp. 3 72h (n=7) **** P

    Journal: Nature

    Article Title: Pharmacological activation of REV-ERBs is lethal in cancer and oncogene induced senescence

    doi: 10.1038/nature25170

    Figure Lengend Snippet: REV-ERBs agonist SR9009 inhibits autophagy a–b , SR9009 treatment reduces the number of autophagosomes, as shown by immunofluorescence of LC3B, (n=biological independent samples MCF7 (n=6 mock), (n=5 SR9009) and T47D (n=5 mock) (n=4 SR9009) Mann–Whitney test one-tailed MCF7 20µM 24h * P =0.0152, T47D 20µM 48h ** P =0.0079; c–d SR9009 induces accumulation of p62 as shown by immunofluorescence; n= biological independent samples MCF7 (n=3 mock), (n=8 SR9009) and T47D (n=5 mock), (n=4 SR9009) Mann–Whitney test one-tailed 48h MCF7 p62 ** P =0.0061; 48h T47D ** P =0.0079; e, Inhibition of autophagy is confirmed by the immunoblot for p62 (20µM 48h, A375); f–g, Inhibition of autophagy precedes apoptosis induction as shown by immunofluorescence of p62, cleaved Caspase 3 and TUNEL assay; n=biological independent samples, Mann–Whitney test one-tailed, A375 20µM Cl. Casp. 3 48h (n=3) * P =0.0179; Cl. Casp. 3 72h (n=7) **** P

    Article Snippet: Apoptosis was evaluated by immunostaining of cleaved caspase 3 (Cell signaling #9664 1:200) and by TUNEL assay using In Situ Cell Death Detection Kit, Fluoresceine or TMR red (Roche).

    Techniques: Immunofluorescence, MANN-WHITNEY, One-tailed Test, Inhibition, TUNEL Assay

    Evaluation of V-9302  in vivo  in HCC1806 cell line xenograft-bearing mice ( A ) Tumor volumetric analysis of mice treated with vehicle or V-9302 (75 mg/kg per day) for 10 days.  n  = 5 mice per group. Treatment started 9 days post injection. P value at day 10 determined by Student’s  t  test. ( B ) Immunofluorescence analysis of tumor tissues harvested from vehicle- or V-9302-treated mice; LC3B and pAKT (Ser473) shown (pink). CD-31 positive vessels shown in green and nuclei in blue (DAPI). 20× magnification. P values determined by Student’s  t  test. ( C ) Effects of V-9302 treatment on pS6-positive cells and caspase 3-positive cells by immunohistochemistry. Quantitative analysis consisted of the mean counts of at least three representative fields from three vehicle and three V-9302-treated mice. For box plots, center line is plotted at the median; the box spans from the first quartile to the third quartile; whiskers represent min to max. Error bars represent ± std. dev.

    Journal: Nature medicine

    Article Title: Pharmacological Blockade of ASCT2-dependent Glutamine Transport Leads To Anti-tumor Efficacy in Preclinical Models

    doi: 10.1038/nm.4464

    Figure Lengend Snippet: Evaluation of V-9302 in vivo in HCC1806 cell line xenograft-bearing mice ( A ) Tumor volumetric analysis of mice treated with vehicle or V-9302 (75 mg/kg per day) for 10 days. n = 5 mice per group. Treatment started 9 days post injection. P value at day 10 determined by Student’s t test. ( B ) Immunofluorescence analysis of tumor tissues harvested from vehicle- or V-9302-treated mice; LC3B and pAKT (Ser473) shown (pink). CD-31 positive vessels shown in green and nuclei in blue (DAPI). 20× magnification. P values determined by Student’s t test. ( C ) Effects of V-9302 treatment on pS6-positive cells and caspase 3-positive cells by immunohistochemistry. Quantitative analysis consisted of the mean counts of at least three representative fields from three vehicle and three V-9302-treated mice. For box plots, center line is plotted at the median; the box spans from the first quartile to the third quartile; whiskers represent min to max. Error bars represent ± std. dev.

    Article Snippet: Tissues were sectioned (5 µm thickness) and stained for pS6 (Cell Signaling, #4858) and caspase 3 (Cell Signaling #9661).

    Techniques: In Vivo, Mouse Assay, Injection, Immunofluorescence, Immunohistochemistry

    Evaluation of V-9302 in vivo ( A ) Pharmacodynamic [ 18 F]-4F-Gln PET imaging prior to and 4 h following a single administration of V-9302 (75 mg/kg) in HCC1806 cell line xenograft-bearing mice (arrows indicate xenograft tumor on right flank*). ( B ) Mean time activity curves (TACs) from tumor regions of interest ( n = 4 measurements per condition); data prior to and 4 hrs following V-9302 administration. ( C ) P values determined by Student’s t test. Quantified tracer accumulation in xenograft tumors, muscle, and liver ( n = 4 measurements per condition). Volumetric analysis over 21 day treatment regimen (Vehicle or V-9302; 75 mg/kg, daily) of HCT-116 ( D ) and HT29 ( F ) cell line xenografts propagated in athymic nude mice ( n = 10 mice per group). Treatment started 12 days post tumor injection for HCT-116 and 4 days post injection for HT29. P values on day 21 determined by Student’s t test. Immunohistochemistry for pS6 and caspase 3 in vehicle-treated or V-9302-treated HCT-116 ( E ) and HT29 ( G ) xenografts. Representative photomicrographs and quantitation shown; magnification 20×. P values determined by Student’s t test. ( H ) Volumetric analysis over 31 day treatment regimen (Vehicle or V-9302; 75 mg/kg, daily) on athymic nude mice bearing patient-derived xenograft tumors (PDX A 008, F3 generation, treatment started 28 days post implantation, KRAS G12V ;p53 R248Q ;PTEN L140Y ; n = 10 mice per group) P value on day 31 determined by Student’s t test. ( I ) Photographs of A 008 PDX-bearing mice treated with V-9302 or vehicle; day 16 of 31. (Error bars represent ± std. dev. *Central photopenia observed.

    Journal: Nature medicine

    Article Title: Pharmacological Blockade of ASCT2-dependent Glutamine Transport Leads To Anti-tumor Efficacy in Preclinical Models

    doi: 10.1038/nm.4464

    Figure Lengend Snippet: Evaluation of V-9302 in vivo ( A ) Pharmacodynamic [ 18 F]-4F-Gln PET imaging prior to and 4 h following a single administration of V-9302 (75 mg/kg) in HCC1806 cell line xenograft-bearing mice (arrows indicate xenograft tumor on right flank*). ( B ) Mean time activity curves (TACs) from tumor regions of interest ( n = 4 measurements per condition); data prior to and 4 hrs following V-9302 administration. ( C ) P values determined by Student’s t test. Quantified tracer accumulation in xenograft tumors, muscle, and liver ( n = 4 measurements per condition). Volumetric analysis over 21 day treatment regimen (Vehicle or V-9302; 75 mg/kg, daily) of HCT-116 ( D ) and HT29 ( F ) cell line xenografts propagated in athymic nude mice ( n = 10 mice per group). Treatment started 12 days post tumor injection for HCT-116 and 4 days post injection for HT29. P values on day 21 determined by Student’s t test. Immunohistochemistry for pS6 and caspase 3 in vehicle-treated or V-9302-treated HCT-116 ( E ) and HT29 ( G ) xenografts. Representative photomicrographs and quantitation shown; magnification 20×. P values determined by Student’s t test. ( H ) Volumetric analysis over 31 day treatment regimen (Vehicle or V-9302; 75 mg/kg, daily) on athymic nude mice bearing patient-derived xenograft tumors (PDX A 008, F3 generation, treatment started 28 days post implantation, KRAS G12V ;p53 R248Q ;PTEN L140Y ; n = 10 mice per group) P value on day 31 determined by Student’s t test. ( I ) Photographs of A 008 PDX-bearing mice treated with V-9302 or vehicle; day 16 of 31. (Error bars represent ± std. dev. *Central photopenia observed.

    Article Snippet: Tissues were sectioned (5 µm thickness) and stained for pS6 (Cell Signaling, #4858) and caspase 3 (Cell Signaling #9661).

    Techniques: In Vivo, Positron Emission Tomography, Imaging, Mouse Assay, Activity Assay, Injection, Immunohistochemistry, Quantitation Assay, Derivative Assay