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synthetic sars cov 2 rna  (ATCC)
94
ATCC synthetic sars cov 2 rna
Workflow of ultrafast one-step qRT-PCR with comparison with conventional qRT-PCR for <t>SARS-CoV-2</t> detection.
Synthetic Sars Cov 2 Rna, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/synthetic sars cov 2 rna/product/ATCC
Average 94 stars, based on 1 article reviews
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synthetic sars cov 2 rna - by Bioz Stars, 2024-10
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Workflow of ultrafast one-step qRT-PCR with comparison with conventional qRT-PCR for SARS-CoV-2 detection.

Journal: Frontiers in Microbiology

Article Title: An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2

doi: 10.3389/fmicb.2021.749783

Figure Lengend Snippet: Workflow of ultrafast one-step qRT-PCR with comparison with conventional qRT-PCR for SARS-CoV-2 detection.

Article Snippet: Using synthetic SARS-CoV-2 RNA from ATCC as the model, we found that 0.4 U of SpeedSTAR HS DNA Polymerase (in 10 μL of qRT-PCR reaction mixture) in the one-step qRT-qPCR assay can detect down to 25 copies of N1 and N2 of SARS-CoV-2 RNA ( ).

Techniques: Quantitative RT-PCR

Limit of detection of ultrafast one-step qRT-PCR assay. SARS-Cov-2 synthetic RNA genome was used as a model sample. RNA templates were series diluted in the range of 25–1 × 10 5 copies. Ultrafast one-step qRT-PCR assay detects both N1 (A) and N2 (B) regions of the nucleocapsid gene of SARS-CoV-2.

Journal: Frontiers in Microbiology

Article Title: An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2

doi: 10.3389/fmicb.2021.749783

Figure Lengend Snippet: Limit of detection of ultrafast one-step qRT-PCR assay. SARS-Cov-2 synthetic RNA genome was used as a model sample. RNA templates were series diluted in the range of 25–1 × 10 5 copies. Ultrafast one-step qRT-PCR assay detects both N1 (A) and N2 (B) regions of the nucleocapsid gene of SARS-CoV-2.

Article Snippet: Using synthetic SARS-CoV-2 RNA from ATCC as the model, we found that 0.4 U of SpeedSTAR HS DNA Polymerase (in 10 μL of qRT-PCR reaction mixture) in the one-step qRT-qPCR assay can detect down to 25 copies of N1 and N2 of SARS-CoV-2 RNA ( ).

Techniques: Quantitative RT-PCR

Cycle threshold (Ct values) of N1 and N2 correlation of ultrafast one-step qRT-PCR with FDA-approved assay “Xpert ® Xpress SARS-CoV-2” for SARS-CoV-2 positive samples. (A) Ct value of N1 and (B) Ct value of N2 from the 30 SARS-CoV-2 positive samples.

Journal: Frontiers in Microbiology

Article Title: An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2

doi: 10.3389/fmicb.2021.749783

Figure Lengend Snippet: Cycle threshold (Ct values) of N1 and N2 correlation of ultrafast one-step qRT-PCR with FDA-approved assay “Xpert ® Xpress SARS-CoV-2” for SARS-CoV-2 positive samples. (A) Ct value of N1 and (B) Ct value of N2 from the 30 SARS-CoV-2 positive samples.

Article Snippet: Using synthetic SARS-CoV-2 RNA from ATCC as the model, we found that 0.4 U of SpeedSTAR HS DNA Polymerase (in 10 μL of qRT-PCR reaction mixture) in the one-step qRT-qPCR assay can detect down to 25 copies of N1 and N2 of SARS-CoV-2 RNA ( ).

Techniques: Quantitative RT-PCR