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    Sino Biological sars cov 2 2019 ncov spike orf mammalian expression plasmid codon optimized covid 19 spike research
    Characterization of human anti-SARS-2 RBD Fabs. ( a ) Soluble ELISA of ten serially diluted human anti-SARS-2 RBD Fabs on immobilized SARS-2 RBD surfaces to measure their apparent affinities ( EC 50 , nM). ( b ) Schematic drawings of a competitive ELISA of human anti-SARS-2 RBD Fabs between the SARS-2 RBD and ACE2 protein (left) or ACE2-overexpressed cells (right). ( c ) Competitive ELISA of human anti-SARS-2 RBD Fabs antagonizing the interaction between ACE2 and the <t>SARS-CoV-2</t> RBD. ( d ) Competitive flow cytometry analysis of human anti-SARS-2 RBD Fabs antagonizing the interaction between ACE2 on cells and the SARS-CoV-2 RBD (tagged with mouse Fc (mFc)). Arrows indicate potentially neutralizing clones. mFc-PE: anti-mouse PE (phycoerythrin) conjugate; MFI: mean fluorescence intensity; n.s: not significant ( p > 0.05); NC: negative control. * and **: p
    Sars Cov 2 2019 Ncov Spike Orf Mammalian Expression Plasmid Codon Optimized Covid 19 Spike Research, supplied by Sino Biological, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sars cov 2 2019 ncov spike orf mammalian expression plasmid codon optimized covid 19 spike research/product/Sino Biological
    Average 98 stars, based on 1 article reviews
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    sars cov 2 2019 ncov spike orf mammalian expression plasmid codon optimized covid 19 spike research - by Bioz Stars, 2021-04
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    Characterization of human anti-SARS-2 RBD Fabs. ( a ) Soluble ELISA of ten serially diluted human anti-SARS-2 RBD Fabs on immobilized SARS-2 RBD surfaces to measure their apparent affinities ( EC 50 , nM). ( b ) Schematic drawings of a competitive ELISA of human anti-SARS-2 RBD Fabs between the SARS-2 RBD and ACE2 protein (left) or ACE2-overexpressed cells (right). ( c ) Competitive ELISA of human anti-SARS-2 RBD Fabs antagonizing the interaction between ACE2 and the SARS-CoV-2 RBD. ( d ) Competitive flow cytometry analysis of human anti-SARS-2 RBD Fabs antagonizing the interaction between ACE2 on cells and the SARS-CoV-2 RBD (tagged with mouse Fc (mFc)). Arrows indicate potentially neutralizing clones. mFc-PE: anti-mouse PE (phycoerythrin) conjugate; MFI: mean fluorescence intensity; n.s: not significant ( p > 0.05); NC: negative control. * and **: p

    Journal: International Journal of Molecular Sciences

    Article Title: Neutralizing Human Antibodies against Severe Acute Respiratory Syndrome Coronavirus 2 Isolated from a Human Synthetic Fab Phage Display Library

    doi: 10.3390/ijms22041913

    Figure Lengend Snippet: Characterization of human anti-SARS-2 RBD Fabs. ( a ) Soluble ELISA of ten serially diluted human anti-SARS-2 RBD Fabs on immobilized SARS-2 RBD surfaces to measure their apparent affinities ( EC 50 , nM). ( b ) Schematic drawings of a competitive ELISA of human anti-SARS-2 RBD Fabs between the SARS-2 RBD and ACE2 protein (left) or ACE2-overexpressed cells (right). ( c ) Competitive ELISA of human anti-SARS-2 RBD Fabs antagonizing the interaction between ACE2 and the SARS-CoV-2 RBD. ( d ) Competitive flow cytometry analysis of human anti-SARS-2 RBD Fabs antagonizing the interaction between ACE2 on cells and the SARS-CoV-2 RBD (tagged with mouse Fc (mFc)). Arrows indicate potentially neutralizing clones. mFc-PE: anti-mouse PE (phycoerythrin) conjugate; MFI: mean fluorescence intensity; n.s: not significant ( p > 0.05); NC: negative control. * and **: p

    Article Snippet: Production of SARS-CoV-2 Spike pseudovirusPlasmids encoding the SARS-CoV-2 spike protein (D614) were purchased from Sino Biological (pCMV3-SARS-CoV-2 Spike, Cat. VG40589-UT, Beijing, China).

    Techniques: Enzyme-linked Immunosorbent Assay, Competitive ELISA, Flow Cytometry, Clone Assay, Fluorescence, Negative Control

    Characterization of anti-SARS-2 RBD immunoglobulin Gs (IgGs). ( a ) Binding analysis of five human anti-SARS-2 RBD IgGs—C12 (IgG), H1 (IgG), C2 (IgG), D12 (IgG), and F7 (IgG)—to the SARS-2 RBD and its variants (top) and the SARS-CoV-2 S1 (D614G) and other coronavirus S1 proteins (bottom), respectively. ( b ) Soluble ELISA of five serially diluted human anti-SARS-2 RBD IgGs on immobilized SARS-2 RBD surfaces to measure their apparent affinities ( EC 50 , nM). ( c ) ELISA detection for five human anti-SARS-2 RBD IgGs blocking the binding of the ACE2 protein with the SARS-CoV-2 RBD (top) and analysis of the flow cytometry for the blocking effect between the SARS-CoV-2 RBD and an ACE2-overexpressed cell (bottom). ( d ) Size-exclusion chromatography analysis of five human anti-SARS-2 RBD IgGs. The positions of the molecular mass markers, shown as kDa, on the retention time x -axis are indicated above the peaks. The data are presented as the mean ± standard error (SEM). MFI: mean fluorescence intensity; NC: negative control; *, **, and ***: p

    Journal: International Journal of Molecular Sciences

    Article Title: Neutralizing Human Antibodies against Severe Acute Respiratory Syndrome Coronavirus 2 Isolated from a Human Synthetic Fab Phage Display Library

    doi: 10.3390/ijms22041913

    Figure Lengend Snippet: Characterization of anti-SARS-2 RBD immunoglobulin Gs (IgGs). ( a ) Binding analysis of five human anti-SARS-2 RBD IgGs—C12 (IgG), H1 (IgG), C2 (IgG), D12 (IgG), and F7 (IgG)—to the SARS-2 RBD and its variants (top) and the SARS-CoV-2 S1 (D614G) and other coronavirus S1 proteins (bottom), respectively. ( b ) Soluble ELISA of five serially diluted human anti-SARS-2 RBD IgGs on immobilized SARS-2 RBD surfaces to measure their apparent affinities ( EC 50 , nM). ( c ) ELISA detection for five human anti-SARS-2 RBD IgGs blocking the binding of the ACE2 protein with the SARS-CoV-2 RBD (top) and analysis of the flow cytometry for the blocking effect between the SARS-CoV-2 RBD and an ACE2-overexpressed cell (bottom). ( d ) Size-exclusion chromatography analysis of five human anti-SARS-2 RBD IgGs. The positions of the molecular mass markers, shown as kDa, on the retention time x -axis are indicated above the peaks. The data are presented as the mean ± standard error (SEM). MFI: mean fluorescence intensity; NC: negative control; *, **, and ***: p

    Article Snippet: Production of SARS-CoV-2 Spike pseudovirusPlasmids encoding the SARS-CoV-2 spike protein (D614) were purchased from Sino Biological (pCMV3-SARS-CoV-2 Spike, Cat. VG40589-UT, Beijing, China).

    Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Blocking Assay, Flow Cytometry, Size-exclusion Chromatography, Fluorescence, Negative Control

    In vitro neutralization assay of human anti-SARS-2 RBD IgGs. Pseudo-typed virus-based neutralization ( a ) and a neutralization assay using authentic SARS-CoV-2 ( b ). ( c ) Correlation in neutralization potencies between pseudo-typed virus- and authentic virus-based assays. ( d ) Correlation between affinities of anti-SARS-2 RBD IgGs and their neutralization potencies for the authentic virus. The data are showed as the mean ± standard error (SEM).

    Journal: International Journal of Molecular Sciences

    Article Title: Neutralizing Human Antibodies against Severe Acute Respiratory Syndrome Coronavirus 2 Isolated from a Human Synthetic Fab Phage Display Library

    doi: 10.3390/ijms22041913

    Figure Lengend Snippet: In vitro neutralization assay of human anti-SARS-2 RBD IgGs. Pseudo-typed virus-based neutralization ( a ) and a neutralization assay using authentic SARS-CoV-2 ( b ). ( c ) Correlation in neutralization potencies between pseudo-typed virus- and authentic virus-based assays. ( d ) Correlation between affinities of anti-SARS-2 RBD IgGs and their neutralization potencies for the authentic virus. The data are showed as the mean ± standard error (SEM).

    Article Snippet: Production of SARS-CoV-2 Spike pseudovirusPlasmids encoding the SARS-CoV-2 spike protein (D614) were purchased from Sino Biological (pCMV3-SARS-CoV-2 Spike, Cat. VG40589-UT, Beijing, China).

    Techniques: In Vitro, Neutralization