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  • 99
    Thermo Fisher tris glycine gel
    Tris Glycine Gel, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4680 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Bio-Rad polyvinylidene fluoride membranes pvdf
    Polyvinylidene Fluoride Membranes Pvdf, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 89/100, based on 3069 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    AvesLabs horseradish peroxidase
    Horseradish Peroxidase, supplied by AvesLabs, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Abcam trek 1 identification
    Effects of <t>TREK-1</t> activation elicited by arachidonic acid (AA) on cellular architecture of primary TM cells. ( A and B ) Representative immunocytochemical images of human normal TM cells probed for TREK-1 (green) and actin (red) after no treatment or AA treatment. ( C ) Quantification of normal TM cells under no treatment or AA treatment conditions using fluorescence intensity/area. The fluorescence intensity was measured as a relative arbitrary unit using ImageJ software under the same settings and conditions for each sample.
    Trek 1 Identification, supplied by Abcam, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Baoding Lead Fluid micro injection system
    Effects of <t>TREK-1</t> activation elicited by arachidonic acid (AA) on cellular architecture of primary TM cells. ( A and B ) Representative immunocytochemical images of human normal TM cells probed for TREK-1 (green) and actin (red) after no treatment or AA treatment. ( C ) Quantification of normal TM cells under no treatment or AA treatment conditions using fluorescence intensity/area. The fluorescence intensity was measured as a relative arbitrary unit using ImageJ software under the same settings and conditions for each sample.
    Micro Injection System, supplied by Baoding Lead Fluid, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Mitutoyo mitutoyo digital caliper
    Effects of <t>TREK-1</t> activation elicited by arachidonic acid (AA) on cellular architecture of primary TM cells. ( A and B ) Representative immunocytochemical images of human normal TM cells probed for TREK-1 (green) and actin (red) after no treatment or AA treatment. ( C ) Quantification of normal TM cells under no treatment or AA treatment conditions using fluorescence intensity/area. The fluorescence intensity was measured as a relative arbitrary unit using ImageJ software under the same settings and conditions for each sample.
    Mitutoyo Digital Caliper, supplied by Mitutoyo, used in various techniques. Bioz Stars score: 89/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Merck & Co sharp dohme inst
    Effects of <t>TREK-1</t> activation elicited by arachidonic acid (AA) on cellular architecture of primary TM cells. ( A and B ) Representative immunocytochemical images of human normal TM cells probed for TREK-1 (green) and actin (red) after no treatment or AA treatment. ( C ) Quantification of normal TM cells under no treatment or AA treatment conditions using fluorescence intensity/area. The fluorescence intensity was measured as a relative arbitrary unit using ImageJ software under the same settings and conditions for each sample.
    Sharp Dohme Inst, supplied by Merck & Co, used in various techniques. Bioz Stars score: 94/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Effects of TREK-1 activation elicited by arachidonic acid (AA) on cellular architecture of primary TM cells. ( A and B ) Representative immunocytochemical images of human normal TM cells probed for TREK-1 (green) and actin (red) after no treatment or AA treatment. ( C ) Quantification of normal TM cells under no treatment or AA treatment conditions using fluorescence intensity/area. The fluorescence intensity was measured as a relative arbitrary unit using ImageJ software under the same settings and conditions for each sample.

    Journal: Scientific Reports

    Article Title: Interaction of cochlin and mechanosensitive channel TREK-1 in trabecular meshwork cells influences the regulation of intraocular pressure

    doi: 10.1038/s41598-017-00430-2

    Figure Lengend Snippet: Effects of TREK-1 activation elicited by arachidonic acid (AA) on cellular architecture of primary TM cells. ( A and B ) Representative immunocytochemical images of human normal TM cells probed for TREK-1 (green) and actin (red) after no treatment or AA treatment. ( C ) Quantification of normal TM cells under no treatment or AA treatment conditions using fluorescence intensity/area. The fluorescence intensity was measured as a relative arbitrary unit using ImageJ software under the same settings and conditions for each sample.

    Article Snippet: For TREK-1 identification, a primary antibody from Abcam was utilized at ~5 μg/mL (TREK-1: cat# ab83932, Abcam, Cambridge, MA).

    Techniques: Activation Assay, Fluorescence, Software

    Interaction between cochlin and TREK-1 results in changes in fluid flow dynamics. ( A ) Cochlin multimerization under shear stress increases the amount of TREK-1 pulled down as shown through western blot analysis. Upper panel shows cochlin with or without shear stress as indicated on a western blot after separation on a non-reducing but denaturing gel, middle and lower panels are identical experiments as above but from reducing and denaturing gels probed with cochlin and TREK-1 antibodies as indicated. ( B ) Co-immunoprecipitation followed by western blot analysis details the interaction of cochlin and TREK-1 in normal as well as glaucomatous samples (the latter is presumably in the presence of shear stress). ( C ) Fluorescein dye migration was measured as fluorescence intensity across several layers of cells on a PVDF membrane within an Ussing-type chamber (as shown in top figure) in the presence of TREK-1 + cochlin, TREK-1 + RPE65, TREK-1 only, TREK-1 shRNA + cochlin as indicated. Student’s t-test showed significant difference in fluorescein transport between TREK-1 + cochlin and all other transfected groups (n = 10; *p

    Journal: Scientific Reports

    Article Title: Interaction of cochlin and mechanosensitive channel TREK-1 in trabecular meshwork cells influences the regulation of intraocular pressure

    doi: 10.1038/s41598-017-00430-2

    Figure Lengend Snippet: Interaction between cochlin and TREK-1 results in changes in fluid flow dynamics. ( A ) Cochlin multimerization under shear stress increases the amount of TREK-1 pulled down as shown through western blot analysis. Upper panel shows cochlin with or without shear stress as indicated on a western blot after separation on a non-reducing but denaturing gel, middle and lower panels are identical experiments as above but from reducing and denaturing gels probed with cochlin and TREK-1 antibodies as indicated. ( B ) Co-immunoprecipitation followed by western blot analysis details the interaction of cochlin and TREK-1 in normal as well as glaucomatous samples (the latter is presumably in the presence of shear stress). ( C ) Fluorescein dye migration was measured as fluorescence intensity across several layers of cells on a PVDF membrane within an Ussing-type chamber (as shown in top figure) in the presence of TREK-1 + cochlin, TREK-1 + RPE65, TREK-1 only, TREK-1 shRNA + cochlin as indicated. Student’s t-test showed significant difference in fluorescein transport between TREK-1 + cochlin and all other transfected groups (n = 10; *p

    Article Snippet: For TREK-1 identification, a primary antibody from Abcam was utilized at ~5 μg/mL (TREK-1: cat# ab83932, Abcam, Cambridge, MA).

    Techniques: Flow Cytometry, Western Blot, Immunoprecipitation, Migration, Fluorescence, shRNA, Transfection

    Whole-cell patch clamp recordings of TREK-1 in HEK293 cells ( A ) Time course of the effect of multimerized cochlin (10 nM) applied to the bath in a HEK293 cell expressing hTREK-1. Cochlin was previously multimerized by Ca 2+ addition for 2 h. Using the whole-cell configuration of the patch clamp technique, TREK-1 current was activated with 1 s voltage ramps from −100 to + 50 mV every 5 s (as in B ). Current values were measured at +45 mV in each ramp and plotted vs. time. Multimerized cochlin (Mu) produced a significant current reduction. In contrast, current increased when the cell was stimulated by shear stress (increase in bath perfusion rate). ( B ) Left: TREK-1 current elicited by a voltage ramp in basal conditions and after addition of multimerized cochlin. Right: an increase in bath perfusion rate activates TREK-1 channels and increases whole cell current. ( C ) Time course of the effect of monomeric (Mo) cochlin using the same protocol as in A. ( D ). Quantification of the effects of cochlin on hTREK-1 current in transiently transfected HEK293 cells: Vehicle (n = 11); monomeric cochlin (Mo; 10 nM; n = 8); monomeric); cochlin multimerized by Ca2+ addition for 2 h (Mu, 10 nM; n = 8) and cochlin multimerized by passing the cochlin solution (10 nM) through a 26 gauge syringe needle 20 times (n = 8). High K + solution: recordings of TREK-1 current in high extracellular K + concentration (135 mM). Vehicle, multimeric cochlin (Mu; 10 nM) and shear stress stimuli were applied (n = 8). SS: shear stress. *p

    Journal: Scientific Reports

    Article Title: Interaction of cochlin and mechanosensitive channel TREK-1 in trabecular meshwork cells influences the regulation of intraocular pressure

    doi: 10.1038/s41598-017-00430-2

    Figure Lengend Snippet: Whole-cell patch clamp recordings of TREK-1 in HEK293 cells ( A ) Time course of the effect of multimerized cochlin (10 nM) applied to the bath in a HEK293 cell expressing hTREK-1. Cochlin was previously multimerized by Ca 2+ addition for 2 h. Using the whole-cell configuration of the patch clamp technique, TREK-1 current was activated with 1 s voltage ramps from −100 to + 50 mV every 5 s (as in B ). Current values were measured at +45 mV in each ramp and plotted vs. time. Multimerized cochlin (Mu) produced a significant current reduction. In contrast, current increased when the cell was stimulated by shear stress (increase in bath perfusion rate). ( B ) Left: TREK-1 current elicited by a voltage ramp in basal conditions and after addition of multimerized cochlin. Right: an increase in bath perfusion rate activates TREK-1 channels and increases whole cell current. ( C ) Time course of the effect of monomeric (Mo) cochlin using the same protocol as in A. ( D ). Quantification of the effects of cochlin on hTREK-1 current in transiently transfected HEK293 cells: Vehicle (n = 11); monomeric cochlin (Mo; 10 nM; n = 8); monomeric); cochlin multimerized by Ca2+ addition for 2 h (Mu, 10 nM; n = 8) and cochlin multimerized by passing the cochlin solution (10 nM) through a 26 gauge syringe needle 20 times (n = 8). High K + solution: recordings of TREK-1 current in high extracellular K + concentration (135 mM). Vehicle, multimeric cochlin (Mu; 10 nM) and shear stress stimuli were applied (n = 8). SS: shear stress. *p

    Article Snippet: For TREK-1 identification, a primary antibody from Abcam was utilized at ~5 μg/mL (TREK-1: cat# ab83932, Abcam, Cambridge, MA).

    Techniques: Patch Clamp, Expressing, Produced, Transfection, Concentration Assay

    TREK-1 silencing inhibits cochlin overexpression increase in IOP. ( A ) Injection of cochlin-expression lentivirus (▲), cochlin-expressing lentivirus with TREK-1 shRNA (■), or TREK-1 shRNA alone (♦) into DBA/2J-Gpnmb+/SjJ mice TM. Analyses of variance (ANOVA) showed a statistically significant difference between the three groups. Scheffe’s post hoc test showed that cochlin alone produced an increase in IOP that was statistically different from the maintenance of a lower IOP in cochlin + TREK-1 shRNA (n = 6; *p

    Journal: Scientific Reports

    Article Title: Interaction of cochlin and mechanosensitive channel TREK-1 in trabecular meshwork cells influences the regulation of intraocular pressure

    doi: 10.1038/s41598-017-00430-2

    Figure Lengend Snippet: TREK-1 silencing inhibits cochlin overexpression increase in IOP. ( A ) Injection of cochlin-expression lentivirus (▲), cochlin-expressing lentivirus with TREK-1 shRNA (■), or TREK-1 shRNA alone (♦) into DBA/2J-Gpnmb+/SjJ mice TM. Analyses of variance (ANOVA) showed a statistically significant difference between the three groups. Scheffe’s post hoc test showed that cochlin alone produced an increase in IOP that was statistically different from the maintenance of a lower IOP in cochlin + TREK-1 shRNA (n = 6; *p

    Article Snippet: For TREK-1 identification, a primary antibody from Abcam was utilized at ~5 μg/mL (TREK-1: cat# ab83932, Abcam, Cambridge, MA).

    Techniques: Over Expression, Injection, Expressing, shRNA, Mouse Assay, Produced

    Whole-cell patch clamp recordings of TREK-1 in TM cells ( A ) TREK-1 current elicited by a voltage ramp from −100 to +50 mV in basal conditions and after addition of multimerized cochlin (10 nM) applied to the bath in a human trabecular meshwork cell (HTM-5) expressing hTREK-1. ( B ) Quantification of the effects of cochlin on hTREK-1 current in transiently transfected HTM-5 cells: multimerized cochlin (Mu, 10 nM; n = 5); shear stress (n = 5) and arachidonic acid (n = 5; 20 µM). *p

    Journal: Scientific Reports

    Article Title: Interaction of cochlin and mechanosensitive channel TREK-1 in trabecular meshwork cells influences the regulation of intraocular pressure

    doi: 10.1038/s41598-017-00430-2

    Figure Lengend Snippet: Whole-cell patch clamp recordings of TREK-1 in TM cells ( A ) TREK-1 current elicited by a voltage ramp from −100 to +50 mV in basal conditions and after addition of multimerized cochlin (10 nM) applied to the bath in a human trabecular meshwork cell (HTM-5) expressing hTREK-1. ( B ) Quantification of the effects of cochlin on hTREK-1 current in transiently transfected HTM-5 cells: multimerized cochlin (Mu, 10 nM; n = 5); shear stress (n = 5) and arachidonic acid (n = 5; 20 µM). *p

    Article Snippet: For TREK-1 identification, a primary antibody from Abcam was utilized at ~5 μg/mL (TREK-1: cat# ab83932, Abcam, Cambridge, MA).

    Techniques: Patch Clamp, Expressing, Transfection