Journal: Frontiers in Immunology
Article Title: G-Protein Coupled Receptor 18 Contributes to Establishment of the CD8 Effector T Cell Compartment
Figure Lengend Snippet: Reduction of KLRG1 + CD8 effector memory (EM) T cells in lymphoid tissues of G-protein coupled receptor 18 (GPR18)-deficient naïve mice (A) Frequency of EM (CD44 hi CD62L lo ), central memory (CM) (CD44 hi CD62L hi ), and naive (CD44 lo CD62L hi ) populations in CD8 + or CD4 + TCRβ + splenocytes in mature mice (6 months old) of the indicated type. Gpr18 +/ − , n = 11; Gpr18 −/− , n = 12. (B) Number of CD8 + TCR β + splenocytes in the indicated mature (6 months old) mice. Each population was pre-gated on CD45 + TCRβ + cells. Gpr18 +/ − , n = 13; Gpr18 −/− , n = 14. (C) Frequency of KLRG1 + cells in CD8 EM splenocytes in indicated mature (6 months old) mice. Gpr18 +/ − , n = 9; Gpr18 −/− , n = 8. (D) Numbers of KLRG1 + CD8 EM splenocytes in indicated mature (6 months old) mice. Gpr18 +/ − , n = 8; Gpr18 −/− , n = 8. (E) Frequency of EM, CM, and naive populations in CD8 + or CD4 + TCRβ + lymphocytes from mesenteric lymph nodes (mLN) in indicated mature (6 months old) mice. Gpr18 +/ − , n = 7; Gpr18 −/− , n = 6. (F) Frequency of KLRG1 + populations in CD8 EM from mLN in indicated mature (6 months old) mice. Gpr18 +/ − , n = 8; Gpr18 −/− , n = 7. (G) Percentages of Gpr18 −/− or control Gpr18 −/− EM cells in gp33 tetramer + CD8 T cells in peripheral blood lymphocytes (PBL) at day 8 and day 30 after lymphocytic choriomeningitis virus (LCMV) Armstrong infection. (H) Percentages of early effector cell (EEC) (KLRG1 lo CD127 lo ), memory precursor effector cells (MPEC) (KLRG1 lo CD127 hi ), and short-lived effector cells (SLEC) (KLRG1 hi CD127 lo ) in gp33 tetramer + CD44 hi CD8 PBL at day 8 and day 30 after LCMV Armstrong infection. (G,H) Gpr18 +/ − , n = 4; Gpr18 −/− , n = 5. Each point represents data from an individual mouse and lines represent means ± SEM (A–H) . *** p
Article Snippet: The following monoclonal antibodies were used for flow cytome-try: TCRβ (H57; BioLegend), CD4 (GK1.5; BioLegend), CD8α (53.6.7; Tonbo Bio), CD8β (H35; eBioscience), CD45.1 (A20; BioLegend), CD45.2 (104; BioLegend), CD62L (MEL-14; BioLegend), CD44 (IM7; BD), KLRG1 (2F1/KLRG1; BioLegend), CD127 (A7R34; eBioscience), CXCR3 (CXCR3-173; BioLegend), IFNγ (XMG1.2; BD), Granzyme B (GB11; Invitrogen), Ki-67 (B56; BD), and T-bet (4B10; BioLegend).
Techniques: Mouse Assay, Infection