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  • 95
    Millipore tryptophan try
    Tryptophan Try, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    BioLegend flow cytome try tcrβ
    Reduction of KLRG1 + CD8 effector memory (EM) T cells in lymphoid tissues of G-protein coupled receptor 18 (GPR18)-deficient naïve mice (A) Frequency of EM (CD44 hi CD62L lo ), central memory (CM) (CD44 hi CD62L hi ), and naive (CD44 lo CD62L hi ) populations in CD8 + or CD4 + <t>TCRβ</t> + splenocytes in mature mice (6 months old) of the indicated type. Gpr18 +/ − , n = 11; Gpr18 −/− , n = 12. (B) Number of CD8 + TCR β + splenocytes in the indicated mature (6 months old) mice. Each population was pre-gated on CD45 + TCRβ + cells. Gpr18 +/ − , n = 13; Gpr18 −/− , n = 14. (C) Frequency of KLRG1 + cells in CD8 EM splenocytes in indicated mature (6 months old) mice. Gpr18 +/ − , n = 9; Gpr18 −/− , n = 8. (D) Numbers of KLRG1 + CD8 EM splenocytes in indicated mature (6 months old) mice. Gpr18 +/ − , n = 8; Gpr18 −/− , n = 8. (E) Frequency of EM, CM, and naive populations in CD8 + or CD4 + TCRβ + lymphocytes from mesenteric lymph nodes (mLN) in indicated mature (6 months old) mice. Gpr18 +/ − , n = 7; Gpr18 −/− , n = 6. (F) Frequency of KLRG1 + populations in CD8 EM from mLN in indicated mature (6 months old) mice. Gpr18 +/ − , n = 8; Gpr18 −/− , n = 7. (G) Percentages of Gpr18 −/− or control Gpr18 −/− EM cells in gp33 tetramer + CD8 T cells in peripheral blood lymphocytes (PBL) at day 8 and day 30 after lymphocytic choriomeningitis virus (LCMV) Armstrong infection. (H) Percentages of early effector cell (EEC) (KLRG1 lo CD127 lo ), memory precursor effector cells (MPEC) (KLRG1 lo CD127 hi ), and short-lived effector cells (SLEC) (KLRG1 hi CD127 lo ) in gp33 tetramer + CD44 hi CD8 PBL at day 8 and day 30 after LCMV Armstrong infection. (G,H) Gpr18 +/ − , n = 4; Gpr18 −/− , n = 5. Each point represents data from an individual mouse and lines represent means ± SEM (A–H) . *** p
    Flow Cytome Try Tcrβ, supplied by BioLegend, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore tryptamine try
    Reduction of KLRG1 + CD8 effector memory (EM) T cells in lymphoid tissues of G-protein coupled receptor 18 (GPR18)-deficient naïve mice (A) Frequency of EM (CD44 hi CD62L lo ), central memory (CM) (CD44 hi CD62L hi ), and naive (CD44 lo CD62L hi ) populations in CD8 + or CD4 + <t>TCRβ</t> + splenocytes in mature mice (6 months old) of the indicated type. Gpr18 +/ − , n = 11; Gpr18 −/− , n = 12. (B) Number of CD8 + TCR β + splenocytes in the indicated mature (6 months old) mice. Each population was pre-gated on CD45 + TCRβ + cells. Gpr18 +/ − , n = 13; Gpr18 −/− , n = 14. (C) Frequency of KLRG1 + cells in CD8 EM splenocytes in indicated mature (6 months old) mice. Gpr18 +/ − , n = 9; Gpr18 −/− , n = 8. (D) Numbers of KLRG1 + CD8 EM splenocytes in indicated mature (6 months old) mice. Gpr18 +/ − , n = 8; Gpr18 −/− , n = 8. (E) Frequency of EM, CM, and naive populations in CD8 + or CD4 + TCRβ + lymphocytes from mesenteric lymph nodes (mLN) in indicated mature (6 months old) mice. Gpr18 +/ − , n = 7; Gpr18 −/− , n = 6. (F) Frequency of KLRG1 + populations in CD8 EM from mLN in indicated mature (6 months old) mice. Gpr18 +/ − , n = 8; Gpr18 −/− , n = 7. (G) Percentages of Gpr18 −/− or control Gpr18 −/− EM cells in gp33 tetramer + CD8 T cells in peripheral blood lymphocytes (PBL) at day 8 and day 30 after lymphocytic choriomeningitis virus (LCMV) Armstrong infection. (H) Percentages of early effector cell (EEC) (KLRG1 lo CD127 lo ), memory precursor effector cells (MPEC) (KLRG1 lo CD127 hi ), and short-lived effector cells (SLEC) (KLRG1 hi CD127 lo ) in gp33 tetramer + CD44 hi CD8 PBL at day 8 and day 30 after LCMV Armstrong infection. (G,H) Gpr18 +/ − , n = 4; Gpr18 −/− , n = 5. Each point represents data from an individual mouse and lines represent means ± SEM (A–H) . *** p
    Tryptamine Try, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Ivoclar Vivadent US variolink ii try in
    Reduction of KLRG1 + CD8 effector memory (EM) T cells in lymphoid tissues of G-protein coupled receptor 18 (GPR18)-deficient naïve mice (A) Frequency of EM (CD44 hi CD62L lo ), central memory (CM) (CD44 hi CD62L hi ), and naive (CD44 lo CD62L hi ) populations in CD8 + or CD4 + <t>TCRβ</t> + splenocytes in mature mice (6 months old) of the indicated type. Gpr18 +/ − , n = 11; Gpr18 −/− , n = 12. (B) Number of CD8 + TCR β + splenocytes in the indicated mature (6 months old) mice. Each population was pre-gated on CD45 + TCRβ + cells. Gpr18 +/ − , n = 13; Gpr18 −/− , n = 14. (C) Frequency of KLRG1 + cells in CD8 EM splenocytes in indicated mature (6 months old) mice. Gpr18 +/ − , n = 9; Gpr18 −/− , n = 8. (D) Numbers of KLRG1 + CD8 EM splenocytes in indicated mature (6 months old) mice. Gpr18 +/ − , n = 8; Gpr18 −/− , n = 8. (E) Frequency of EM, CM, and naive populations in CD8 + or CD4 + TCRβ + lymphocytes from mesenteric lymph nodes (mLN) in indicated mature (6 months old) mice. Gpr18 +/ − , n = 7; Gpr18 −/− , n = 6. (F) Frequency of KLRG1 + populations in CD8 EM from mLN in indicated mature (6 months old) mice. Gpr18 +/ − , n = 8; Gpr18 −/− , n = 7. (G) Percentages of Gpr18 −/− or control Gpr18 −/− EM cells in gp33 tetramer + CD8 T cells in peripheral blood lymphocytes (PBL) at day 8 and day 30 after lymphocytic choriomeningitis virus (LCMV) Armstrong infection. (H) Percentages of early effector cell (EEC) (KLRG1 lo CD127 lo ), memory precursor effector cells (MPEC) (KLRG1 lo CD127 hi ), and short-lived effector cells (SLEC) (KLRG1 hi CD127 lo ) in gp33 tetramer + CD44 hi CD8 PBL at day 8 and day 30 after LCMV Armstrong infection. (G,H) Gpr18 +/ − , n = 4; Gpr18 −/− , n = 5. Each point represents data from an individual mouse and lines represent means ± SEM (A–H) . *** p
    Variolink Ii Try In, supplied by Ivoclar Vivadent US, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Reduction of KLRG1 + CD8 effector memory (EM) T cells in lymphoid tissues of G-protein coupled receptor 18 (GPR18)-deficient naïve mice (A) Frequency of EM (CD44 hi CD62L lo ), central memory (CM) (CD44 hi CD62L hi ), and naive (CD44 lo CD62L hi ) populations in CD8 + or CD4 + TCRβ + splenocytes in mature mice (6 months old) of the indicated type. Gpr18 +/ − , n = 11; Gpr18 −/− , n = 12. (B) Number of CD8 + TCR β + splenocytes in the indicated mature (6 months old) mice. Each population was pre-gated on CD45 + TCRβ + cells. Gpr18 +/ − , n = 13; Gpr18 −/− , n = 14. (C) Frequency of KLRG1 + cells in CD8 EM splenocytes in indicated mature (6 months old) mice. Gpr18 +/ − , n = 9; Gpr18 −/− , n = 8. (D) Numbers of KLRG1 + CD8 EM splenocytes in indicated mature (6 months old) mice. Gpr18 +/ − , n = 8; Gpr18 −/− , n = 8. (E) Frequency of EM, CM, and naive populations in CD8 + or CD4 + TCRβ + lymphocytes from mesenteric lymph nodes (mLN) in indicated mature (6 months old) mice. Gpr18 +/ − , n = 7; Gpr18 −/− , n = 6. (F) Frequency of KLRG1 + populations in CD8 EM from mLN in indicated mature (6 months old) mice. Gpr18 +/ − , n = 8; Gpr18 −/− , n = 7. (G) Percentages of Gpr18 −/− or control Gpr18 −/− EM cells in gp33 tetramer + CD8 T cells in peripheral blood lymphocytes (PBL) at day 8 and day 30 after lymphocytic choriomeningitis virus (LCMV) Armstrong infection. (H) Percentages of early effector cell (EEC) (KLRG1 lo CD127 lo ), memory precursor effector cells (MPEC) (KLRG1 lo CD127 hi ), and short-lived effector cells (SLEC) (KLRG1 hi CD127 lo ) in gp33 tetramer + CD44 hi CD8 PBL at day 8 and day 30 after LCMV Armstrong infection. (G,H) Gpr18 +/ − , n = 4; Gpr18 −/− , n = 5. Each point represents data from an individual mouse and lines represent means ± SEM (A–H) . *** p

    Journal: Frontiers in Immunology

    Article Title: G-Protein Coupled Receptor 18 Contributes to Establishment of the CD8 Effector T Cell Compartment

    doi: 10.3389/fimmu.2018.00660

    Figure Lengend Snippet: Reduction of KLRG1 + CD8 effector memory (EM) T cells in lymphoid tissues of G-protein coupled receptor 18 (GPR18)-deficient naïve mice (A) Frequency of EM (CD44 hi CD62L lo ), central memory (CM) (CD44 hi CD62L hi ), and naive (CD44 lo CD62L hi ) populations in CD8 + or CD4 + TCRβ + splenocytes in mature mice (6 months old) of the indicated type. Gpr18 +/ − , n = 11; Gpr18 −/− , n = 12. (B) Number of CD8 + TCR β + splenocytes in the indicated mature (6 months old) mice. Each population was pre-gated on CD45 + TCRβ + cells. Gpr18 +/ − , n = 13; Gpr18 −/− , n = 14. (C) Frequency of KLRG1 + cells in CD8 EM splenocytes in indicated mature (6 months old) mice. Gpr18 +/ − , n = 9; Gpr18 −/− , n = 8. (D) Numbers of KLRG1 + CD8 EM splenocytes in indicated mature (6 months old) mice. Gpr18 +/ − , n = 8; Gpr18 −/− , n = 8. (E) Frequency of EM, CM, and naive populations in CD8 + or CD4 + TCRβ + lymphocytes from mesenteric lymph nodes (mLN) in indicated mature (6 months old) mice. Gpr18 +/ − , n = 7; Gpr18 −/− , n = 6. (F) Frequency of KLRG1 + populations in CD8 EM from mLN in indicated mature (6 months old) mice. Gpr18 +/ − , n = 8; Gpr18 −/− , n = 7. (G) Percentages of Gpr18 −/− or control Gpr18 −/− EM cells in gp33 tetramer + CD8 T cells in peripheral blood lymphocytes (PBL) at day 8 and day 30 after lymphocytic choriomeningitis virus (LCMV) Armstrong infection. (H) Percentages of early effector cell (EEC) (KLRG1 lo CD127 lo ), memory precursor effector cells (MPEC) (KLRG1 lo CD127 hi ), and short-lived effector cells (SLEC) (KLRG1 hi CD127 lo ) in gp33 tetramer + CD44 hi CD8 PBL at day 8 and day 30 after LCMV Armstrong infection. (G,H) Gpr18 +/ − , n = 4; Gpr18 −/− , n = 5. Each point represents data from an individual mouse and lines represent means ± SEM (A–H) . *** p

    Article Snippet: The following monoclonal antibodies were used for flow cytome-try: TCRβ (H57; BioLegend), CD4 (GK1.5; BioLegend), CD8α (53.6.7; Tonbo Bio), CD8β (H35; eBioscience), CD45.1 (A20; BioLegend), CD45.2 (104; BioLegend), CD62L (MEL-14; BioLegend), CD44 (IM7; BD), KLRG1 (2F1/KLRG1; BioLegend), CD127 (A7R34; eBioscience), CXCR3 (CXCR3-173; BioLegend), IFNγ (XMG1.2; BD), Granzyme B (GB11; Invitrogen), Ki-67 (B56; BD), and T-bet (4B10; BioLegend).

    Techniques: Mouse Assay, Infection

    Rescue effects of G-protein-coupled receptor 18 (GPR18) expression on CD8 effector memory (EM) cells in Gpr18 −/− mice. (A,B) CD45.2 + Gpr18 −/− bone marrow transduced with empty vector or Gpr18 retrovirus was used to reconstitute CD45.1 + recipients ( n = 5). Donor cells were gated on CD45.1 − CD45.2 + and then gated on GFP + (transduced donor cells) or GFP − (untransduced donor cells). (A) CD8 + TCRβ + cells from peripheral blood lymphocytes (PBL) (left panel) or spleen (right panel) were stained for naïve (CD44 lo CD62L hi ), EM (CD44 hi CD62L lo ), and CM (CD44 hi CD62L hi ). Percentage of EM, CM, and naïve populations in GFP + donor cells were presented as a ratio to those in GFP − donor cells from the same animal. (B) CD8 EM cells from PBL (left panel) or spleen (right panel) were stained for KLRG1. Percentage of KLRG1 + populations in GFP + donor cells were presented as a ratio to those in GFP − donor cells from the same animal. Each symbol represents an individual mouse, and lines represent means ± SEM. *** p

    Journal: Frontiers in Immunology

    Article Title: G-Protein Coupled Receptor 18 Contributes to Establishment of the CD8 Effector T Cell Compartment

    doi: 10.3389/fimmu.2018.00660

    Figure Lengend Snippet: Rescue effects of G-protein-coupled receptor 18 (GPR18) expression on CD8 effector memory (EM) cells in Gpr18 −/− mice. (A,B) CD45.2 + Gpr18 −/− bone marrow transduced with empty vector or Gpr18 retrovirus was used to reconstitute CD45.1 + recipients ( n = 5). Donor cells were gated on CD45.1 − CD45.2 + and then gated on GFP + (transduced donor cells) or GFP − (untransduced donor cells). (A) CD8 + TCRβ + cells from peripheral blood lymphocytes (PBL) (left panel) or spleen (right panel) were stained for naïve (CD44 lo CD62L hi ), EM (CD44 hi CD62L lo ), and CM (CD44 hi CD62L hi ). Percentage of EM, CM, and naïve populations in GFP + donor cells were presented as a ratio to those in GFP − donor cells from the same animal. (B) CD8 EM cells from PBL (left panel) or spleen (right panel) were stained for KLRG1. Percentage of KLRG1 + populations in GFP + donor cells were presented as a ratio to those in GFP − donor cells from the same animal. Each symbol represents an individual mouse, and lines represent means ± SEM. *** p

    Article Snippet: The following monoclonal antibodies were used for flow cytome-try: TCRβ (H57; BioLegend), CD4 (GK1.5; BioLegend), CD8α (53.6.7; Tonbo Bio), CD8β (H35; eBioscience), CD45.1 (A20; BioLegend), CD45.2 (104; BioLegend), CD62L (MEL-14; BioLegend), CD44 (IM7; BD), KLRG1 (2F1/KLRG1; BioLegend), CD127 (A7R34; eBioscience), CXCR3 (CXCR3-173; BioLegend), IFNγ (XMG1.2; BD), Granzyme B (GB11; Invitrogen), Ki-67 (B56; BD), and T-bet (4B10; BioLegend).

    Techniques: Expressing, Mouse Assay, Transduction, Plasmid Preparation, Staining

    Reduction of KLRG1 + CD8 EM T cells in G-protein-coupled receptor 18 (GPR18)-deficient peripheral blood lymphocytes (PBL). (A) Gpr18 transcript abundance in the indicated cell subsets relative to Hprt . Each point indicates cells sorted from an individual mouse and lines indicate mean ± SEM. n = 3 or 4 in each populations. EM, effector-memory; CM, central memory; IEL, intraepithelial lymphocytes. (B) Flow cytometric analysis of CD44 and CD62L expression in CD8 + TCRβ + PBL from the indicated mature (6 months old) mice. Numbers show percentage of cells in the indicated gate. (C) Frequency of EM (CD44 hi CD62L lo ), CM (CD44 hi CD62L hi ), and naive (CD44 lo CD62L hi ) CD8 + or CD4 + TCRβ + cells in PBL from young (2 months old, left panel) or mature (6 months old, right panel) Gpr18 +/ − and Gpr18 −/− mice. Left panel: Gpr18 +/ − , n = 8; Gpr18 −/− , n = 8. Right panel: Gpr18 +/ − , n = 21; Gpr18 −/− , n = 16. (D) Flow cytometric analysis of KLRG1 expression in CD8 EM PBL from the indicated mature (6 months old) mice. Numbers show percentage of cells in the indicated gate. (E) Frequency of KLRG1 + CD8 EM PBL in indicated young (2 months old) or mature (6 months old) mice. Young adults: Gpr18 +/ − , n = 8; Gpr18 −/− , n = 8. Mature adults: Gpr18 +/ − , n = 21; Gpr18 −/− , n = 16. Each point represents data from an individual mouse and lines represent means ± SEM (C,E) . *** p

    Journal: Frontiers in Immunology

    Article Title: G-Protein Coupled Receptor 18 Contributes to Establishment of the CD8 Effector T Cell Compartment

    doi: 10.3389/fimmu.2018.00660

    Figure Lengend Snippet: Reduction of KLRG1 + CD8 EM T cells in G-protein-coupled receptor 18 (GPR18)-deficient peripheral blood lymphocytes (PBL). (A) Gpr18 transcript abundance in the indicated cell subsets relative to Hprt . Each point indicates cells sorted from an individual mouse and lines indicate mean ± SEM. n = 3 or 4 in each populations. EM, effector-memory; CM, central memory; IEL, intraepithelial lymphocytes. (B) Flow cytometric analysis of CD44 and CD62L expression in CD8 + TCRβ + PBL from the indicated mature (6 months old) mice. Numbers show percentage of cells in the indicated gate. (C) Frequency of EM (CD44 hi CD62L lo ), CM (CD44 hi CD62L hi ), and naive (CD44 lo CD62L hi ) CD8 + or CD4 + TCRβ + cells in PBL from young (2 months old, left panel) or mature (6 months old, right panel) Gpr18 +/ − and Gpr18 −/− mice. Left panel: Gpr18 +/ − , n = 8; Gpr18 −/− , n = 8. Right panel: Gpr18 +/ − , n = 21; Gpr18 −/− , n = 16. (D) Flow cytometric analysis of KLRG1 expression in CD8 EM PBL from the indicated mature (6 months old) mice. Numbers show percentage of cells in the indicated gate. (E) Frequency of KLRG1 + CD8 EM PBL in indicated young (2 months old) or mature (6 months old) mice. Young adults: Gpr18 +/ − , n = 8; Gpr18 −/− , n = 8. Mature adults: Gpr18 +/ − , n = 21; Gpr18 −/− , n = 16. Each point represents data from an individual mouse and lines represent means ± SEM (C,E) . *** p

    Article Snippet: The following monoclonal antibodies were used for flow cytome-try: TCRβ (H57; BioLegend), CD4 (GK1.5; BioLegend), CD8α (53.6.7; Tonbo Bio), CD8β (H35; eBioscience), CD45.1 (A20; BioLegend), CD45.2 (104; BioLegend), CD62L (MEL-14; BioLegend), CD44 (IM7; BD), KLRG1 (2F1/KLRG1; BioLegend), CD127 (A7R34; eBioscience), CXCR3 (CXCR3-173; BioLegend), IFNγ (XMG1.2; BD), Granzyme B (GB11; Invitrogen), Ki-67 (B56; BD), and T-bet (4B10; BioLegend).

    Techniques: Flow Cytometry, Expressing, Mouse Assay

    Cell intrinsic defects of KLRG1 + CD8 T cells in G-protein coupled receptor 18 (GPR18)-deficiency. B6-CD45.1 + mice were reconstituted with CD45.1/2 + WT and CD45.2 + Gpr18 +/ − or Gpr18 −/− bone marrow (BM) 10 weeks before analysis ( n = 6). (A) Flow cytometric analysis of CD44 and CD62L expression in CD8 + TCRβ + and congenic (CD45) marker + splenocytes from the indicated donor cells in the same animal. Numbers show percentage of cells in the indicated gate. (B) Naïve (CD44 lo CD62L hi ), effector memory (EM) (CD44 hi CD62L lo ), and CM (CD44 hi CD62L hi ) T cells in Gpr18 +/ − or Gpr18 −/− CD8 + TCRβ + cells, identified as in (A), were presented as a ratio to the WT control donor cells from the same animal in peripheral blood lymphocytes (PBL) (upper panel) or spleen (lower panel). (C) Flow cytometric analysis of KLRG1 expression in CD8 EM splenocytes from the indicated donor cells in the same animal. (D) Percentage of KLRG1 + cells in Gpr18 +/ − or Gpr18 −/− CD8 + EM TCRβ + cells, determined as in (C) , presented as a ratio to the WT control donor cells from the same animal in PBL (upper panel) or spleen (lower panel). Each symbol in (B,D) represents an individual mouse, and lines represent means ± SEM. * p

    Journal: Frontiers in Immunology

    Article Title: G-Protein Coupled Receptor 18 Contributes to Establishment of the CD8 Effector T Cell Compartment

    doi: 10.3389/fimmu.2018.00660

    Figure Lengend Snippet: Cell intrinsic defects of KLRG1 + CD8 T cells in G-protein coupled receptor 18 (GPR18)-deficiency. B6-CD45.1 + mice were reconstituted with CD45.1/2 + WT and CD45.2 + Gpr18 +/ − or Gpr18 −/− bone marrow (BM) 10 weeks before analysis ( n = 6). (A) Flow cytometric analysis of CD44 and CD62L expression in CD8 + TCRβ + and congenic (CD45) marker + splenocytes from the indicated donor cells in the same animal. Numbers show percentage of cells in the indicated gate. (B) Naïve (CD44 lo CD62L hi ), effector memory (EM) (CD44 hi CD62L lo ), and CM (CD44 hi CD62L hi ) T cells in Gpr18 +/ − or Gpr18 −/− CD8 + TCRβ + cells, identified as in (A), were presented as a ratio to the WT control donor cells from the same animal in peripheral blood lymphocytes (PBL) (upper panel) or spleen (lower panel). (C) Flow cytometric analysis of KLRG1 expression in CD8 EM splenocytes from the indicated donor cells in the same animal. (D) Percentage of KLRG1 + cells in Gpr18 +/ − or Gpr18 −/− CD8 + EM TCRβ + cells, determined as in (C) , presented as a ratio to the WT control donor cells from the same animal in PBL (upper panel) or spleen (lower panel). Each symbol in (B,D) represents an individual mouse, and lines represent means ± SEM. * p

    Article Snippet: The following monoclonal antibodies were used for flow cytome-try: TCRβ (H57; BioLegend), CD4 (GK1.5; BioLegend), CD8α (53.6.7; Tonbo Bio), CD8β (H35; eBioscience), CD45.1 (A20; BioLegend), CD45.2 (104; BioLegend), CD62L (MEL-14; BioLegend), CD44 (IM7; BD), KLRG1 (2F1/KLRG1; BioLegend), CD127 (A7R34; eBioscience), CXCR3 (CXCR3-173; BioLegend), IFNγ (XMG1.2; BD), Granzyme B (GB11; Invitrogen), Ki-67 (B56; BD), and T-bet (4B10; BioLegend).

    Techniques: Mouse Assay, Flow Cytometry, Expressing, Marker