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  • 93
    Vector Laboratories secondary antibody texas red conjugated goat anti rabbit igg
    Secondary Antibody Texas Red Conjugated Goat Anti Rabbit Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories texas red horse anti-mouse igg antibody
    Texas Red Horse Anti Mouse Igg Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories texas red conjugated horse anti mouse igg secondary antibody
    Localisation of germinal centre B-cell expressed transcript 2 (GCET2) in COS7 and B cells by confocal microscopy. (A) Localisation of GCET2 in COS7 cells. This figure shows the negative control. (B) Localisation of GCET2 in COS7 cells by confocal analysis. Wild-type GCET2 was mainly localised in the cell membrane with some distribution in the cytosol, probably in the Golgi apparatus and the membrane of cytosolic organelles. (C) Localisation of wild-type GCET2 in COS7 cells. (D) Localisation of GCET2 in DHL16 cells. DHL16 cells without pMIG-GCET2 transduction were used as negative control. Cells were stained using mouse anti-V5 monoclonal primary antibody and Texas <t>red</t> <t>conjugated</t> horse anti-mouse <t>IgG</t> secondary antibody. This picture is the Differential Interface Contrast (DIC) of control DHL16 B cells. (E) Fluorescence of control DHL16 cells. The cells were totally negative for red signals. (F) Combination (overlay) of G and H. (G) DIC of pMIG-GCET2 transduced B cell. (H) Red fluorescence of pMIG-GCET2 transduced B cells. Red signals were predominantly in the cell membrane and the Golgi area. (I) Combination (overlay) of J and K.
    Texas Red Conjugated Horse Anti Mouse Igg Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/texas red conjugated horse anti mouse igg secondary antibody/product/Vector Laboratories
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    Proteintech mouse anti glyt1
    Bupivacaine regulated <t>GlyT1</t> and BDNF expression in astrocytes. ( A , B ) Representative western blotting and quantification of GlyT1 and BDNF expression. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. the control group. Data are expressed as the mean ± SEM. Original images of gels/blots are available in Supplementary Fig . Bup, Bupivacaine.
    Mouse Anti Glyt1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    VHG Labs icp ms metal standards
    Bupivacaine regulated <t>GlyT1</t> and BDNF expression in astrocytes. ( A , B ) Representative western blotting and quantification of GlyT1 and BDNF expression. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. the control group. Data are expressed as the mean ± SEM. Original images of gels/blots are available in Supplementary Fig . Bup, Bupivacaine.
    Icp Ms Metal Standards, supplied by VHG Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Localisation of germinal centre B-cell expressed transcript 2 (GCET2) in COS7 and B cells by confocal microscopy. (A) Localisation of GCET2 in COS7 cells. This figure shows the negative control. (B) Localisation of GCET2 in COS7 cells by confocal analysis. Wild-type GCET2 was mainly localised in the cell membrane with some distribution in the cytosol, probably in the Golgi apparatus and the membrane of cytosolic organelles. (C) Localisation of wild-type GCET2 in COS7 cells. (D) Localisation of GCET2 in DHL16 cells. DHL16 cells without pMIG-GCET2 transduction were used as negative control. Cells were stained using mouse anti-V5 monoclonal primary antibody and Texas red conjugated horse anti-mouse IgG secondary antibody. This picture is the Differential Interface Contrast (DIC) of control DHL16 B cells. (E) Fluorescence of control DHL16 cells. The cells were totally negative for red signals. (F) Combination (overlay) of G and H. (G) DIC of pMIG-GCET2 transduced B cell. (H) Red fluorescence of pMIG-GCET2 transduced B cells. Red signals were predominantly in the cell membrane and the Golgi area. (I) Combination (overlay) of J and K.

    Journal:

    Article Title: Studies of a germinal centre B-cell expressed gene, GCET2 , suggest its role as a membrane associated adapter protein

    doi: 10.1111/j.1365-2141.2007.06597.x

    Figure Lengend Snippet: Localisation of germinal centre B-cell expressed transcript 2 (GCET2) in COS7 and B cells by confocal microscopy. (A) Localisation of GCET2 in COS7 cells. This figure shows the negative control. (B) Localisation of GCET2 in COS7 cells by confocal analysis. Wild-type GCET2 was mainly localised in the cell membrane with some distribution in the cytosol, probably in the Golgi apparatus and the membrane of cytosolic organelles. (C) Localisation of wild-type GCET2 in COS7 cells. (D) Localisation of GCET2 in DHL16 cells. DHL16 cells without pMIG-GCET2 transduction were used as negative control. Cells were stained using mouse anti-V5 monoclonal primary antibody and Texas red conjugated horse anti-mouse IgG secondary antibody. This picture is the Differential Interface Contrast (DIC) of control DHL16 B cells. (E) Fluorescence of control DHL16 cells. The cells were totally negative for red signals. (F) Combination (overlay) of G and H. (G) DIC of pMIG-GCET2 transduced B cell. (H) Red fluorescence of pMIG-GCET2 transduced B cells. Red signals were predominantly in the cell membrane and the Golgi area. (I) Combination (overlay) of J and K.

    Article Snippet: Slides were incubated in anti-V5 primary antibody (5 µg/ml) for 1 h at room temperature with gentle agitation, and in Texas Red conjugated horse anti-mouse IgG secondary antibody (7·5 µg/ml; Vector Laboratories) for 1 h at room temperature in the dark with gentle agitation.

    Techniques: Confocal Microscopy, Negative Control, Transduction, Staining, Fluorescence

    Bupivacaine regulated GlyT1 and BDNF expression in astrocytes. ( A , B ) Representative western blotting and quantification of GlyT1 and BDNF expression. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. the control group. Data are expressed as the mean ± SEM. Original images of gels/blots are available in Supplementary Fig . Bup, Bupivacaine.

    Journal: Scientific Reports

    Article Title: Bupivacaine reduces GlyT1 expression by potentiating the p-AMPKα/BDNF signalling pathway in spinal astrocytes of rats

    doi: 10.1038/s41598-022-05478-3

    Figure Lengend Snippet: Bupivacaine regulated GlyT1 and BDNF expression in astrocytes. ( A , B ) Representative western blotting and quantification of GlyT1 and BDNF expression. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. the control group. Data are expressed as the mean ± SEM. Original images of gels/blots are available in Supplementary Fig . Bup, Bupivacaine.

    Article Snippet: All the cells were blocked with 5% goat serum in 0.01 mM phosphate-buffered saline (PBS, pH 7.4) with 0.3% Triton-X-100 for 2 g h at room temperature and then incubated overnight at 4 °C with primary antibodies, including mouse anti-GlyT1 (1:25; Proteintech Group, Inc.), mouse anti-BDNF (1:50; Proteintech Group, Inc.), and rabbit anti-p-AMPKα (Thr172) (1:50; Affinity Biosciences, Inc.).

    Techniques: Expressing, Western Blot

    Effects of 7,8-DHF (BDNF receptor agonist) on GlyT1 in astrocytes. ( A ) Protein expression of GlyT1 in astrocytes after treatment with 7,8-DHF was measured by western blotting. ( B ) Representative image and fluorescence intensity quantification of the GlyT1 expression using immunofluorescence staining. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. the control group. Data are expressed as the mean ± SEM. Original images of gels/blots are available in Supplementary Fig .

    Journal: Scientific Reports

    Article Title: Bupivacaine reduces GlyT1 expression by potentiating the p-AMPKα/BDNF signalling pathway in spinal astrocytes of rats

    doi: 10.1038/s41598-022-05478-3

    Figure Lengend Snippet: Effects of 7,8-DHF (BDNF receptor agonist) on GlyT1 in astrocytes. ( A ) Protein expression of GlyT1 in astrocytes after treatment with 7,8-DHF was measured by western blotting. ( B ) Representative image and fluorescence intensity quantification of the GlyT1 expression using immunofluorescence staining. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. the control group. Data are expressed as the mean ± SEM. Original images of gels/blots are available in Supplementary Fig .

    Article Snippet: All the cells were blocked with 5% goat serum in 0.01 mM phosphate-buffered saline (PBS, pH 7.4) with 0.3% Triton-X-100 for 2 g h at room temperature and then incubated overnight at 4 °C with primary antibodies, including mouse anti-GlyT1 (1:25; Proteintech Group, Inc.), mouse anti-BDNF (1:50; Proteintech Group, Inc.), and rabbit anti-p-AMPKα (Thr172) (1:50; Affinity Biosciences, Inc.).

    Techniques: Expressing, Western Blot, Fluorescence, Immunofluorescence, Staining

    Schematic diagram of the signalling pathways involved in bupivacaine induced GlyT1 reduction in spinal astrocytes. Bupivacaine activated AMPK, and increased the expression of the downstream molecule BDNF to inhibit GlyT1 expression.

    Journal: Scientific Reports

    Article Title: Bupivacaine reduces GlyT1 expression by potentiating the p-AMPKα/BDNF signalling pathway in spinal astrocytes of rats

    doi: 10.1038/s41598-022-05478-3

    Figure Lengend Snippet: Schematic diagram of the signalling pathways involved in bupivacaine induced GlyT1 reduction in spinal astrocytes. Bupivacaine activated AMPK, and increased the expression of the downstream molecule BDNF to inhibit GlyT1 expression.

    Article Snippet: All the cells were blocked with 5% goat serum in 0.01 mM phosphate-buffered saline (PBS, pH 7.4) with 0.3% Triton-X-100 for 2 g h at room temperature and then incubated overnight at 4 °C with primary antibodies, including mouse anti-GlyT1 (1:25; Proteintech Group, Inc.), mouse anti-BDNF (1:50; Proteintech Group, Inc.), and rabbit anti-p-AMPKα (Thr172) (1:50; Affinity Biosciences, Inc.).

    Techniques: Expressing