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    • d5 tg 16 0 18 0 16 0  (Avanti Polar)
    94
    Avanti Polar d5 tg 16 0 18 0 16 0
    D5 Tg 16 0 18 0 16 0, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/d5 tg 16 0 18 0 16 0/product/Avanti Polar
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    d5 tg 16 0 18 0 16 0 - by Bioz Stars, 2023-05
    94/100 stars
    		  Buy from Supplier
    thapsigargin tg  (Selleck Chemicals)
    94
    Selleck Chemicals thapsigargin tg
    Inactivation of TFEB and NF- κ B contributes to suppression of SASP by Mar-M. (A) Western blot analysis of the P65, the phspho-p65 (p-P65) in PC3/Doc cells treated with Mar-M. (B) Immunoblots of p-P65 state in nuclear and cytosolic fraction of PC3/Doc incubated with Mar-M for 1 or 5 days. GAPDH and Histone 3 (H3) were used as control. (C) The nuclear translocation of P65 was detected by confocal microscopy, LPS was used as positive control. (D) The nuclear and cytoplasmic fraction of TFEB and TFE3 in PC3/Doc cells and PC3 cells. (E) Western blot analysis of the TFEB and TFE3 in PC3/Doc cells treated with Mar-M. (F) Nuclear fraction of TFEB in PC3/Doc cells with Mar-M, <t>thapsigargin</t> (Tg) and Starvation (Sv). (G) Immunofluorescent staining of TFEB-GFP detected in 293T cells treated with Mar-M for 24 h and stained with DAPI (to visualize nuclei). Starvation and thapsigargin were used as positive controls for TFEB nuclear translation. (H) siRNA knockdown of TFEB in PC3/Doc cells was performed and analysed the IL-1α 、 IL-1β and IL-6 levels. (I) Quantification of IL-1α 、 IL-1β and IL-6 levels were shown after TFEB overexpression in 293T cell treated with Mar-M. Results are representative of three independent experiments. Data are mean ± SD, * P < 0.05, ** P < 0.01 and *** P < 0.001. Scale bar: 20 μm.
    Thapsigargin Tg, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thapsigargin tg/product/Selleck Chemicals
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    thapsigargin tg - by Bioz Stars, 2023-05
    94/100 stars
    		  Buy from Supplier
    gene exp tg hs00174974 m1  (Thermo Fisher)
    88
    Thermo Fisher gene exp tg hs00174974 m1
    Inactivation of TFEB and NF- κ B contributes to suppression of SASP by Mar-M. (A) Western blot analysis of the P65, the phspho-p65 (p-P65) in PC3/Doc cells treated with Mar-M. (B) Immunoblots of p-P65 state in nuclear and cytosolic fraction of PC3/Doc incubated with Mar-M for 1 or 5 days. GAPDH and Histone 3 (H3) were used as control. (C) The nuclear translocation of P65 was detected by confocal microscopy, LPS was used as positive control. (D) The nuclear and cytoplasmic fraction of TFEB and TFE3 in PC3/Doc cells and PC3 cells. (E) Western blot analysis of the TFEB and TFE3 in PC3/Doc cells treated with Mar-M. (F) Nuclear fraction of TFEB in PC3/Doc cells with Mar-M, <t>thapsigargin</t> (Tg) and Starvation (Sv). (G) Immunofluorescent staining of TFEB-GFP detected in 293T cells treated with Mar-M for 24 h and stained with DAPI (to visualize nuclei). Starvation and thapsigargin were used as positive controls for TFEB nuclear translation. (H) siRNA knockdown of TFEB in PC3/Doc cells was performed and analysed the IL-1α 、 IL-1β and IL-6 levels. (I) Quantification of IL-1α 、 IL-1β and IL-6 levels were shown after TFEB overexpression in 293T cell treated with Mar-M. Results are representative of three independent experiments. Data are mean ± SD, * P < 0.05, ** P < 0.01 and *** P < 0.001. Scale bar: 20 μm.
    Gene Exp Tg Hs00174974 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp tg hs00174974 m1/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gene exp tg hs00174974 m1 - by Bioz Stars, 2023-05
    88/100 stars
    		  Buy from Supplier
    d5 tg internal standard mixture  (Avanti Polar)
    94
    Avanti Polar d5 tg internal standard mixture
    Inactivation of TFEB and NF- κ B contributes to suppression of SASP by Mar-M. (A) Western blot analysis of the P65, the phspho-p65 (p-P65) in PC3/Doc cells treated with Mar-M. (B) Immunoblots of p-P65 state in nuclear and cytosolic fraction of PC3/Doc incubated with Mar-M for 1 or 5 days. GAPDH and Histone 3 (H3) were used as control. (C) The nuclear translocation of P65 was detected by confocal microscopy, LPS was used as positive control. (D) The nuclear and cytoplasmic fraction of TFEB and TFE3 in PC3/Doc cells and PC3 cells. (E) Western blot analysis of the TFEB and TFE3 in PC3/Doc cells treated with Mar-M. (F) Nuclear fraction of TFEB in PC3/Doc cells with Mar-M, <t>thapsigargin</t> (Tg) and Starvation (Sv). (G) Immunofluorescent staining of TFEB-GFP detected in 293T cells treated with Mar-M for 24 h and stained with DAPI (to visualize nuclei). Starvation and thapsigargin were used as positive controls for TFEB nuclear translation. (H) siRNA knockdown of TFEB in PC3/Doc cells was performed and analysed the IL-1α 、 IL-1β and IL-6 levels. (I) Quantification of IL-1α 、 IL-1β and IL-6 levels were shown after TFEB overexpression in 293T cell treated with Mar-M. Results are representative of three independent experiments. Data are mean ± SD, * P < 0.05, ** P < 0.01 and *** P < 0.001. Scale bar: 20 μm.
    D5 Tg Internal Standard Mixture, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/d5 tg internal standard mixture/product/Avanti Polar
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    d5 tg internal standard mixture - by Bioz Stars, 2023-05
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Inactivation of TFEB and NF- κ B contributes to suppression of SASP by Mar-M. (A) Western blot analysis of the P65, the phspho-p65 (p-P65) in PC3/Doc cells treated with Mar-M. (B) Immunoblots of p-P65 state in nuclear and cytosolic fraction of PC3/Doc incubated with Mar-M for 1 or 5 days. GAPDH and Histone 3 (H3) were used as control. (C) The nuclear translocation of P65 was detected by confocal microscopy, LPS was used as positive control. (D) The nuclear and cytoplasmic fraction of TFEB and TFE3 in PC3/Doc cells and PC3 cells. (E) Western blot analysis of the TFEB and TFE3 in PC3/Doc cells treated with Mar-M. (F) Nuclear fraction of TFEB in PC3/Doc cells with Mar-M, thapsigargin (Tg) and Starvation (Sv). (G) Immunofluorescent staining of TFEB-GFP detected in 293T cells treated with Mar-M for 24 h and stained with DAPI (to visualize nuclei). Starvation and thapsigargin were used as positive controls for TFEB nuclear translation. (H) siRNA knockdown of TFEB in PC3/Doc cells was performed and analysed the IL-1α 、 IL-1β and IL-6 levels. (I) Quantification of IL-1α 、 IL-1β and IL-6 levels were shown after TFEB overexpression in 293T cell treated with Mar-M. Results are representative of three independent experiments. Data are mean ± SD, * P < 0.05, ** P < 0.01 and *** P < 0.001. Scale bar: 20 μm.

    Journal: Acta Pharmaceutica Sinica. B

    Article Title: Inactivation of TFEB and NF- κ B by marchantin M alleviates the chemotherapy-driven pro-tumorigenic senescent secretion

    doi: 10.1016/j.apsb.2019.08.007

    Figure Lengend Snippet: Inactivation of TFEB and NF- κ B contributes to suppression of SASP by Mar-M. (A) Western blot analysis of the P65, the phspho-p65 (p-P65) in PC3/Doc cells treated with Mar-M. (B) Immunoblots of p-P65 state in nuclear and cytosolic fraction of PC3/Doc incubated with Mar-M for 1 or 5 days. GAPDH and Histone 3 (H3) were used as control. (C) The nuclear translocation of P65 was detected by confocal microscopy, LPS was used as positive control. (D) The nuclear and cytoplasmic fraction of TFEB and TFE3 in PC3/Doc cells and PC3 cells. (E) Western blot analysis of the TFEB and TFE3 in PC3/Doc cells treated with Mar-M. (F) Nuclear fraction of TFEB in PC3/Doc cells with Mar-M, thapsigargin (Tg) and Starvation (Sv). (G) Immunofluorescent staining of TFEB-GFP detected in 293T cells treated with Mar-M for 24 h and stained with DAPI (to visualize nuclei). Starvation and thapsigargin were used as positive controls for TFEB nuclear translation. (H) siRNA knockdown of TFEB in PC3/Doc cells was performed and analysed the IL-1α 、 IL-1β and IL-6 levels. (I) Quantification of IL-1α 、 IL-1β and IL-6 levels were shown after TFEB overexpression in 293T cell treated with Mar-M. Results are representative of three independent experiments. Data are mean ± SD, * P < 0.05, ** P < 0.01 and *** P < 0.001. Scale bar: 20 μm.

    Article Snippet: Thapsigargin (Tg) was purchased from Selleck (Houston, TX, USA).

    Techniques: Western Blot, Incubation, Translocation Assay, Confocal Microscopy, Positive Control, Staining, Over Expression