Journal: Journal of Neurophysiology
Article Title: Dynamic Metabotropic Control of Intrinsic Firing in Cerebellar Unipolar Brush Cells
Figure Lengend Snippet: t-ACPD affects 2 distinct potassium currents. A : current-clamp recording from a UBC in acute cerebellar slice. Tertiapin depolarized the cells when delivered on top of t-ACPD, but never reversed the mGluR-induced blockade of firing (although full recovery was always obtained on t-ACPD washout). The plot in B shows the values of UBCs' membrane potential measured in the presence of t-ACPD and in the presence of both t-ACPD and tertiapin (at the time points indicated by the arrows in A ; the values were −96.9 ± 7 mV in t-ACPD and −89.5 ± 5.6 mV in t-ACPD + tertiapin, 7 cells, P
Article Snippet: Drugs and chemicals were from Sigma (St. Louis, MO), except 4-ethylphenylamino-1,2-dimethyl-6-methylaminopyrimidinium chloride (ZD7288), (2 S )-2-amino-2-[(1 S ,2 S )-2-carboxyxycloprop-1-yl]-3-(xanth-9-yl) propanoic acid , (±)-aminocyclopentane- trans -1,3-dicarboxylic acid (t-ACPD), (2 R ,4 R )-4-aminopyrrolidine-2,4-dicarboxylate [(2 R ,4 R )APDC; Tocris, Ellisville, MO], tetrodotoxin (TTX), and r-tertiapin (cat #RTT250; Alomone Labs, Jerusalem, Israel).