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    • ω conotoxin cnviia  (Alomone Labs)
    92
    Alomone Labs ω conotoxin cnviia
    Mature eggs were incubated for 1 hr with <t>ω-Conotoxin</t> <t>CnVIIA</t> and fertilized with BDF1 sperm in the same medium for 8 h. (A) Two pronuclear zygotes were collected and cultured in KSOM for five days. (B) Fertilization rates were calculated at 2 cells per MII mature eggs, and the blastocyst rate were calculated from 2-cells. * p <0.05.
    ω Conotoxin Cnviia, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ω conotoxin cnviia/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
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    ω conotoxin cnviia - by Bioz Stars, 2023-02
    92/100 stars
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    Mature eggs were incubated for 1 hr with ω-Conotoxin CnVIIA and fertilized with BDF1 sperm in the same medium for 8 h. (A) Two pronuclear zygotes were collected and cultured in KSOM for five days. (B) Fertilization rates were calculated at 2 cells per MII mature eggs, and the blastocyst rate were calculated from 2-cells. * p <0.05.

    Journal: Development & Reproduction

    Article Title: Voltage Dependent N Type Calcium Channel in Mouse Egg Fertilization

    doi: 10.12717/DR.2020.24.4.297

    Figure Lengend Snippet: Mature eggs were incubated for 1 hr with ω-Conotoxin CnVIIA and fertilized with BDF1 sperm in the same medium for 8 h. (A) Two pronuclear zygotes were collected and cultured in KSOM for five days. (B) Fertilization rates were calculated at 2 cells per MII mature eggs, and the blastocyst rate were calculated from 2-cells. * p <0.05.

    Article Snippet: Sperm was collected from epididymis of 12-week-old male mice in HTF supplemented with 5% KnockOut™ Serum Replacement (KSR, Gibco, Grand Island, NY, USA) and capacitated for 30 min. Capacitated spermatozoa were added (1–2×10 6 / mL) to eggs in HTF medium and incubated for 6 h with or without ω-Conotoxin CnVIIA, a specific Blocker of N-Type [Ca 2+ ]i Channels (Alomone Labs, Jerusalem, Israel).

    Techniques: Incubation, Cell Culture