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    • ω conotoxin cnviia  (Alomone Labs)
    93
    Alomone Labs ω conotoxin cnviia
    Effect of N-type voltage dependent calcium channel (VDCC) blocker, <t>ω-Conotoxin</t> <t>CnVIIA,</t> on fertilization and embryonic development. Mature eggs were incubated for 1 hr with ω-Conotoxin CnVIIA and fertilized with BDF1 sperm in the same medium for 8 h. (A) Two pronuclear zygotes were collected and cultured in KSOM for five days. (B) Fertilization rates were calculated at 2 cells per MII mature eggs, and the blastocyst rate were calculated from 2-cells. * p
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    Effect of N-type voltage dependent calcium channel (VDCC) blocker, ω-Conotoxin CnVIIA, on fertilization and embryonic development. Mature eggs were incubated for 1 hr with ω-Conotoxin CnVIIA and fertilized with BDF1 sperm in the same medium for 8 h. (A) Two pronuclear zygotes were collected and cultured in KSOM for five days. (B) Fertilization rates were calculated at 2 cells per MII mature eggs, and the blastocyst rate were calculated from 2-cells. * p

    Journal: Development & Reproduction

    Article Title: Voltage Dependent N Type Calcium Channel in Mouse Egg Fertilization

    doi: 10.12717/DR.2020.24.4.297

    Figure Lengend Snippet: Effect of N-type voltage dependent calcium channel (VDCC) blocker, ω-Conotoxin CnVIIA, on fertilization and embryonic development. Mature eggs were incubated for 1 hr with ω-Conotoxin CnVIIA and fertilized with BDF1 sperm in the same medium for 8 h. (A) Two pronuclear zygotes were collected and cultured in KSOM for five days. (B) Fertilization rates were calculated at 2 cells per MII mature eggs, and the blastocyst rate were calculated from 2-cells. * p

    Article Snippet: In vitro fertilization and differential staining of blastocyst Sperm was collected from epididymis of 12-week-old male mice in HTF supplemented with 5% KnockOut™ Serum Replacement (KSR, Gibco, Grand Island, NY, USA) and capacitated for 30 min. Capacitated spermatozoa were added (1–2×106 / mL) to eggs in HTF medium and incubated for 6 h with or without ω-Conotoxin CnVIIA, a specific Blocker of N-Type [Ca2+]i Channels (Alomone Labs, Jerusalem, Israel).

    Techniques: Incubation, Cell Culture

    Effect of N-type voltage dependent calcium channel (VDCC) blocker, ω-Conotoxin CnVIIA, on [Ca 2+ ]i oscillation by SrCl egg activation. (A) 10 mM SrCl in [Ca 2+ ]i free TL-HEPES medium. Parenthesis in each group represents egg number /total egg number. (B) Relative fluorescence intensity was calculated using the basal level of [Ca 2+ ] and the amplitude of [Ca 2+ ]i spikes (max-min). The number of spikes per 2 h and duration of each spike were determined from (A). Each dot represents the number of eggs.

    Journal: Development & Reproduction

    Article Title: Voltage Dependent N Type Calcium Channel in Mouse Egg Fertilization

    doi: 10.12717/DR.2020.24.4.297

    Figure Lengend Snippet: Effect of N-type voltage dependent calcium channel (VDCC) blocker, ω-Conotoxin CnVIIA, on [Ca 2+ ]i oscillation by SrCl egg activation. (A) 10 mM SrCl in [Ca 2+ ]i free TL-HEPES medium. Parenthesis in each group represents egg number /total egg number. (B) Relative fluorescence intensity was calculated using the basal level of [Ca 2+ ] and the amplitude of [Ca 2+ ]i spikes (max-min). The number of spikes per 2 h and duration of each spike were determined from (A). Each dot represents the number of eggs.

    Article Snippet: In vitro fertilization and differential staining of blastocyst Sperm was collected from epididymis of 12-week-old male mice in HTF supplemented with 5% KnockOut™ Serum Replacement (KSR, Gibco, Grand Island, NY, USA) and capacitated for 30 min. Capacitated spermatozoa were added (1–2×106 / mL) to eggs in HTF medium and incubated for 6 h with or without ω-Conotoxin CnVIIA, a specific Blocker of N-Type [Ca2+]i Channels (Alomone Labs, Jerusalem, Israel).

    Techniques: Activation Assay, Fluorescence