Journal: The European Journal of Neuroscience
Article Title: MAP6 interacts with Tctex1 and Cav2.2/N‐type calcium channels to regulate calcium signalling in neurons
Figure Lengend Snippet: Specificity of Ca v 2‐type calcium channels deficit in MAP6 KO neurons. (A) Left panel, examples of KCl‐stimulated fluo‐4‐loaded WT cortical neurons in the absence (black line) or presence (yellow line) of 20 μ m nimodipine. Right panels, ratios of KCl‐elicited fluorescence intensity peaks, recorded from WT (white squares) and MAP6 KO (grey squares) cortical neurons in the presence or absence of 20 μ m nimodipine (yellow curves), 180 n m ω‐agatoxin IVA (red curves) or 320 n m ω‐conotoxin GVIA (green curves). n represents the total number of wells recorded from eight independent neuronal cultures. ns, P > 0.05, *, P
Article Snippet: Automatic plate reading Twenty‐four‐well plates of cortical cultures after 7–8 DIV were incubated at 37 °C without CO2 , in the presence of 1 μm Fluo‐4 (Thermo Fisher Scientific, France) in warm aCSF containing LiCl + KA, in the absence [nimo] or presence [toxins] of 20 μm nimodipine (see above, ), during 20 min. Each well was rinsed with aCSF containing LiCl + KA, without [nimo] or with [toxins] 20 mm nimodipine and 450 μL of fresh medium containing also DMSO or 20 μm nimodipine [nimo] on the one hand or DMSO, 320 nm ω‐conotoxin GVIA (STC‐750, Alomone Labs, Israel, diluted to 160 μm in DMSO) or 180 nm ω‐agatoxin IVA (STC‐750, Alomone Labs, Israel, diluted to 90 μm in DMSO) [toxins] on the other hand, was added to the corresponding wells.