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  • 99
    Millipore shrna targeting stap 2
    Shrna Targeting Stap 2, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shrna targeting stap 2/product/Millipore
    Average 99 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    shrna targeting stap 2 - by Bioz Stars, 2020-11
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    94
    Millipore antibodies against stap 1
    <t>STAP-1</t> affects JAK/STAT signaling as well as PPAR signaling pathways. Gene expression profiles of CML LSCs at day 11 after first BMT between WT and STAP-1 KO mice were compared by RNA-seq analysis. a Scatter plot represents the mean of normalized counts. In all, 3792 genes (2536 downregulated genes; 1256 upregulated genes) were differentially expressed (twofold change) in STAP-1 KO LSCs ( y -axis) relative to WT LSCs ( x -axis). b – d Gene expression data were subjected to bioinformatics analysis by Ingenuity Pathway Analysis. For each signaling pathway, an enrichment p value and a z -score of activation were calculated. Pathways that were predicted to be downregulated or upregulated in STAP-1 KO CML LSCs compared with WT CML LSCs are shown in ( b ). Pathways involved in inflammatory responses are shown in ( c ). Upstream regulatory candidates are shown in ( d ). Candidates of interest are labeled.
    Antibodies Against Stap 1, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against stap 1/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against stap 1 - by Bioz Stars, 2020-11
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    85
    Millipore anti stap2 antibody
    <t>STAP-1</t> affects JAK/STAT signaling as well as PPAR signaling pathways. Gene expression profiles of CML LSCs at day 11 after first BMT between WT and STAP-1 KO mice were compared by RNA-seq analysis. a Scatter plot represents the mean of normalized counts. In all, 3792 genes (2536 downregulated genes; 1256 upregulated genes) were differentially expressed (twofold change) in STAP-1 KO LSCs ( y -axis) relative to WT LSCs ( x -axis). b – d Gene expression data were subjected to bioinformatics analysis by Ingenuity Pathway Analysis. For each signaling pathway, an enrichment p value and a z -score of activation were calculated. Pathways that were predicted to be downregulated or upregulated in STAP-1 KO CML LSCs compared with WT CML LSCs are shown in ( b ). Pathways involved in inflammatory responses are shown in ( c ). Upstream regulatory candidates are shown in ( d ). Candidates of interest are labeled.
    Anti Stap2 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti stap2 antibody/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti stap2 antibody - by Bioz Stars, 2020-11
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    Image Search Results


    STAP-1 affects JAK/STAT signaling as well as PPAR signaling pathways. Gene expression profiles of CML LSCs at day 11 after first BMT between WT and STAP-1 KO mice were compared by RNA-seq analysis. a Scatter plot represents the mean of normalized counts. In all, 3792 genes (2536 downregulated genes; 1256 upregulated genes) were differentially expressed (twofold change) in STAP-1 KO LSCs ( y -axis) relative to WT LSCs ( x -axis). b – d Gene expression data were subjected to bioinformatics analysis by Ingenuity Pathway Analysis. For each signaling pathway, an enrichment p value and a z -score of activation were calculated. Pathways that were predicted to be downregulated or upregulated in STAP-1 KO CML LSCs compared with WT CML LSCs are shown in ( b ). Pathways involved in inflammatory responses are shown in ( c ). Upstream regulatory candidates are shown in ( d ). Candidates of interest are labeled.

    Journal: Oncogene

    Article Title: Signal-transducing adapter protein-1 is required for maintenance of leukemic stem cells in CML

    doi: 10.1038/s41388-020-01387-9

    Figure Lengend Snippet: STAP-1 affects JAK/STAT signaling as well as PPAR signaling pathways. Gene expression profiles of CML LSCs at day 11 after first BMT between WT and STAP-1 KO mice were compared by RNA-seq analysis. a Scatter plot represents the mean of normalized counts. In all, 3792 genes (2536 downregulated genes; 1256 upregulated genes) were differentially expressed (twofold change) in STAP-1 KO LSCs ( y -axis) relative to WT LSCs ( x -axis). b – d Gene expression data were subjected to bioinformatics analysis by Ingenuity Pathway Analysis. For each signaling pathway, an enrichment p value and a z -score of activation were calculated. Pathways that were predicted to be downregulated or upregulated in STAP-1 KO CML LSCs compared with WT CML LSCs are shown in ( b ). Pathways involved in inflammatory responses are shown in ( c ). Upstream regulatory candidates are shown in ( d ). Candidates of interest are labeled.

    Article Snippet: Antibodies against STAP-1 (rabbit polyclonal) were purchased from Sigma-Aldrich.

    Techniques: Expressing, Mouse Assay, RNA Sequencing Assay, Activation Assay, Labeling

    STAP-1 is a possible therapeutic target in CML. a Lin − CD34 + BM cells were collected from newly diagnosed chronic CML patients ( n = 6), and cultured in triplicates in liquid medium with or without 1 μM imatinib for 24 h. Total RNA samples isolated from these cells were quantified by real-time RT-PCR ( n = 6 per group). Results are normalized to the untreated cells. Data are shown as mean ± SD. NS not significant. b Model for the role of STAP-1 in CML LSCs. STAP-1 binds to BCR-ABL and STAT5. STAP-1 positively regulates STAT5 phosphorylation status followed by change in cellular apoptosis and drug sensitivity in CML.

    Journal: Oncogene

    Article Title: Signal-transducing adapter protein-1 is required for maintenance of leukemic stem cells in CML

    doi: 10.1038/s41388-020-01387-9

    Figure Lengend Snippet: STAP-1 is a possible therapeutic target in CML. a Lin − CD34 + BM cells were collected from newly diagnosed chronic CML patients ( n = 6), and cultured in triplicates in liquid medium with or without 1 μM imatinib for 24 h. Total RNA samples isolated from these cells were quantified by real-time RT-PCR ( n = 6 per group). Results are normalized to the untreated cells. Data are shown as mean ± SD. NS not significant. b Model for the role of STAP-1 in CML LSCs. STAP-1 binds to BCR-ABL and STAT5. STAP-1 positively regulates STAT5 phosphorylation status followed by change in cellular apoptosis and drug sensitivity in CML.

    Article Snippet: Antibodies against STAP-1 (rabbit polyclonal) were purchased from Sigma-Aldrich.

    Techniques: Cell Culture, Isolation, Quantitative RT-PCR

    STAP-1 regulates the expression of antiapoptotic proteins via STAT5 signaling pathway. a Phosphorylation status of AKT (pS473), ERK1/2 (pT202/pY204), P38MAPK (pT180/pY182), STAT3 (pY705), and STAT5 (pY649) in GFP + cells from WT or STAP-1 KO CML mice, at day 11 after first BMT, were analyzed by flow cytometry. Histograms show the mean fluorescence intensity (MFI) on the x -axis and events normalized to Mode on the y -axis. Histograms are representative of three independent experiments. Bar graph represents MFI of phosphorylated STAT5 ( n = 7 per group). b Phosphorylation status of STAT5 (pY649) in LSCs from WT or STAP-1 KO CML mice was analyzed by flow cytometry. Histograms are representative of three independent experiments (left panel). Bar graph represents MFI of phosphorylated STAT5 ( n = 8 per group). c mRNA expression of STAT5 target genes was measured by real-time RT-PCR in CML LSCs from primary CML mice ( n = 7 per group). Results are normalized to WT mice. d STAP-1 KO CML LSCs were transduced with constitutively active STAT5A, and mRNA expression of Bcl-2 and Bcl-xL was measured by real-time RT-PCR ( n = 3). Results are normalized to control. Data are shown as mean ± SD. * p

    Journal: Oncogene

    Article Title: Signal-transducing adapter protein-1 is required for maintenance of leukemic stem cells in CML

    doi: 10.1038/s41388-020-01387-9

    Figure Lengend Snippet: STAP-1 regulates the expression of antiapoptotic proteins via STAT5 signaling pathway. a Phosphorylation status of AKT (pS473), ERK1/2 (pT202/pY204), P38MAPK (pT180/pY182), STAT3 (pY705), and STAT5 (pY649) in GFP + cells from WT or STAP-1 KO CML mice, at day 11 after first BMT, were analyzed by flow cytometry. Histograms show the mean fluorescence intensity (MFI) on the x -axis and events normalized to Mode on the y -axis. Histograms are representative of three independent experiments. Bar graph represents MFI of phosphorylated STAT5 ( n = 7 per group). b Phosphorylation status of STAT5 (pY649) in LSCs from WT or STAP-1 KO CML mice was analyzed by flow cytometry. Histograms are representative of three independent experiments (left panel). Bar graph represents MFI of phosphorylated STAT5 ( n = 8 per group). c mRNA expression of STAT5 target genes was measured by real-time RT-PCR in CML LSCs from primary CML mice ( n = 7 per group). Results are normalized to WT mice. d STAP-1 KO CML LSCs were transduced with constitutively active STAT5A, and mRNA expression of Bcl-2 and Bcl-xL was measured by real-time RT-PCR ( n = 3). Results are normalized to control. Data are shown as mean ± SD. * p

    Article Snippet: Antibodies against STAP-1 (rabbit polyclonal) were purchased from Sigma-Aldrich.

    Techniques: Expressing, Mouse Assay, Flow Cytometry, Fluorescence, Quantitative RT-PCR, Transduction

    STAP-1 regulates STAT5 via JAK and PPAR signaling in CML. a STAP-1 knockdown CML cell lines were established using short hairpin RNA. Total RNA samples isolated from these cells were quantified by real-time RT-PCR. Data represent the levels of these mRNAs normalized to control (sh control). Data of a representative experiment, which was repeated at least three times with similar results, are shown. b Phosphorylation status of STAT5 (pY649) in CML cell lines was analyzed by flow cytometry. Bar graph represents MFI of phosphorylated STAT5 normalized to sh control cells ( n = 6). CML cell lines were treated with imatinib ( c ) or ruxolitinib ( d ) at the indicated concentrations for 24 h. Growth inhibitory effect on these cells was determined. The data represent the means of triplicate experiments. Similar results were obtained for three independent experiments. e 293T cells were transfected with STAT5a, STAT5b, or JAK2 in either presence or absence of Myc-tagged STAP-1. The cells were lysed and immunoprecipitated with anti-Myc antibody. An aliquot of total cell lysate (TCL) and the immunoprecipitates (IP) were blotted with anti-STAT5a, anti-STAT5b, anti-JAK2, anti-Myc, or anti-STAP-1 antibody. f STAP-1 knockdown CML cell lines were either or not treated with pioglitazone at 50 μM for 96 h and phosphorylation status of STAT5 (pY649) was analyzed. Data of a representative experiment, which was repeated at least three times with similar results, are shown. Data represent the mean ± SD. * p

    Journal: Oncogene

    Article Title: Signal-transducing adapter protein-1 is required for maintenance of leukemic stem cells in CML

    doi: 10.1038/s41388-020-01387-9

    Figure Lengend Snippet: STAP-1 regulates STAT5 via JAK and PPAR signaling in CML. a STAP-1 knockdown CML cell lines were established using short hairpin RNA. Total RNA samples isolated from these cells were quantified by real-time RT-PCR. Data represent the levels of these mRNAs normalized to control (sh control). Data of a representative experiment, which was repeated at least three times with similar results, are shown. b Phosphorylation status of STAT5 (pY649) in CML cell lines was analyzed by flow cytometry. Bar graph represents MFI of phosphorylated STAT5 normalized to sh control cells ( n = 6). CML cell lines were treated with imatinib ( c ) or ruxolitinib ( d ) at the indicated concentrations for 24 h. Growth inhibitory effect on these cells was determined. The data represent the means of triplicate experiments. Similar results were obtained for three independent experiments. e 293T cells were transfected with STAT5a, STAT5b, or JAK2 in either presence or absence of Myc-tagged STAP-1. The cells were lysed and immunoprecipitated with anti-Myc antibody. An aliquot of total cell lysate (TCL) and the immunoprecipitates (IP) were blotted with anti-STAT5a, anti-STAT5b, anti-JAK2, anti-Myc, or anti-STAP-1 antibody. f STAP-1 knockdown CML cell lines were either or not treated with pioglitazone at 50 μM for 96 h and phosphorylation status of STAT5 (pY649) was analyzed. Data of a representative experiment, which was repeated at least three times with similar results, are shown. Data represent the mean ± SD. * p

    Article Snippet: Antibodies against STAP-1 (rabbit polyclonal) were purchased from Sigma-Aldrich.

    Techniques: shRNA, Isolation, Quantitative RT-PCR, Flow Cytometry, Transfection, Immunoprecipitation

    The expression of STAP-1 aberrantly increased in human CML LSC subset. a , b BM cells were collected from healthy donor ( n = 7) and newly diagnosed chronic CML patients ( n = 8), and Lin − CD34 + CD38 − hematopoietic stem cells and Lin − CD34 + CD38 + hematopoietic progenitor cells were sorted using flow cytometry. a The representative data of flow cytometry are shown. b STAP-1 and STAP-2 mRNA were measured by real-time RT-PCR in the indicated subsets. Bar graph shows expression levels of STAP-1 and STAP-2 mRNAs normalized to data from MNC fraction of healthy donor. c Lin − CD34 + BM cells from newly diagnosed chronic CML patients ( n = 6) were transduced with control or STAP-1 shRNA lentivirus, cultured in triplicates in methylcellulose medium for 14 days, and colonies were counted. Data are shown as mean ± SD. * p

    Journal: Oncogene

    Article Title: Signal-transducing adapter protein-1 is required for maintenance of leukemic stem cells in CML

    doi: 10.1038/s41388-020-01387-9

    Figure Lengend Snippet: The expression of STAP-1 aberrantly increased in human CML LSC subset. a , b BM cells were collected from healthy donor ( n = 7) and newly diagnosed chronic CML patients ( n = 8), and Lin − CD34 + CD38 − hematopoietic stem cells and Lin − CD34 + CD38 + hematopoietic progenitor cells were sorted using flow cytometry. a The representative data of flow cytometry are shown. b STAP-1 and STAP-2 mRNA were measured by real-time RT-PCR in the indicated subsets. Bar graph shows expression levels of STAP-1 and STAP-2 mRNAs normalized to data from MNC fraction of healthy donor. c Lin − CD34 + BM cells from newly diagnosed chronic CML patients ( n = 6) were transduced with control or STAP-1 shRNA lentivirus, cultured in triplicates in methylcellulose medium for 14 days, and colonies were counted. Data are shown as mean ± SD. * p

    Article Snippet: Antibodies against STAP-1 (rabbit polyclonal) were purchased from Sigma-Aldrich.

    Techniques: Expressing, Flow Cytometry, Quantitative RT-PCR, Transduction, shRNA, Cell Culture

    STAP-1 does not affect normal murine hematopoiesis. a The indicated subsets of hematopoietic cells derived from WT C57BL/6 mice were analyzed for STAP-1 expression by real-time RT-PCR ( n = 3). STAP-1 transcript levels were normalized to myeloid subset. b Blood counts (WT, n = 5; STAP-1 KO, n = 7) and biochemical parameters (WT, n = 4; STAP-1, KO n = 4) were evaluated using peripheral blood. c The frequency of LSK and LT-HSC (CD48 − CD150 + LSK) cells from WT and STAP-1 KO mice was analyzed. Representative flow cytometry plots for identification of LT-HSC were shown (left panels). Bar graph represents mean proportion of LSK cells or LT-HSCs (WT, n = 9; STAP-1 KO, n = 8). d LSK cells from WT or STAP-1 KO BM ( n = 6) were cultured in methylcellulose medium for 7 days, colonies were counted, and cells were serially replated. e WT or STAP-1 KO LSK cells (5000 cells, Ly5.2) were transplanted into lethally irradiated recipients (Ly5.1). At 4 months after transplantation, 2 × 10 6 whole BM cells from the primary recipient mice were transplanted into the secondary recipient mice (Ly5.1). The left line graphs represent the mean percentages of donor chimerism in recipient’s PB at the indicated time points after transplantation (WT, n = 9; STAP-1 KO, n = 7 for the first BMT, and n = 11 per group for the second BMT). Upper bar graphs represent chimerism of donor-derived cells in BMMNC cells and LSK cells from primary BMT recipients of WT or STAP-1 KO LSK cells 4 months after primary BMT (WT, n = 9; STAP-1 KO, n = 7). Lower bar graph represents differentiation status (B220 + B cells, CD3 + T cells, or Mac-1/Gr-1 + myeloid cells) in BM cells from primary BMT recipients of WT or STAP-1 KO LSK cells 4 months after primary BMT (WT, n = 9; STAP-1 KO, n = 7). Data shown represent the mean ± SD. * p

    Journal: Oncogene

    Article Title: Signal-transducing adapter protein-1 is required for maintenance of leukemic stem cells in CML

    doi: 10.1038/s41388-020-01387-9

    Figure Lengend Snippet: STAP-1 does not affect normal murine hematopoiesis. a The indicated subsets of hematopoietic cells derived from WT C57BL/6 mice were analyzed for STAP-1 expression by real-time RT-PCR ( n = 3). STAP-1 transcript levels were normalized to myeloid subset. b Blood counts (WT, n = 5; STAP-1 KO, n = 7) and biochemical parameters (WT, n = 4; STAP-1, KO n = 4) were evaluated using peripheral blood. c The frequency of LSK and LT-HSC (CD48 − CD150 + LSK) cells from WT and STAP-1 KO mice was analyzed. Representative flow cytometry plots for identification of LT-HSC were shown (left panels). Bar graph represents mean proportion of LSK cells or LT-HSCs (WT, n = 9; STAP-1 KO, n = 8). d LSK cells from WT or STAP-1 KO BM ( n = 6) were cultured in methylcellulose medium for 7 days, colonies were counted, and cells were serially replated. e WT or STAP-1 KO LSK cells (5000 cells, Ly5.2) were transplanted into lethally irradiated recipients (Ly5.1). At 4 months after transplantation, 2 × 10 6 whole BM cells from the primary recipient mice were transplanted into the secondary recipient mice (Ly5.1). The left line graphs represent the mean percentages of donor chimerism in recipient’s PB at the indicated time points after transplantation (WT, n = 9; STAP-1 KO, n = 7 for the first BMT, and n = 11 per group for the second BMT). Upper bar graphs represent chimerism of donor-derived cells in BMMNC cells and LSK cells from primary BMT recipients of WT or STAP-1 KO LSK cells 4 months after primary BMT (WT, n = 9; STAP-1 KO, n = 7). Lower bar graph represents differentiation status (B220 + B cells, CD3 + T cells, or Mac-1/Gr-1 + myeloid cells) in BM cells from primary BMT recipients of WT or STAP-1 KO LSK cells 4 months after primary BMT (WT, n = 9; STAP-1 KO, n = 7). Data shown represent the mean ± SD. * p

    Article Snippet: Antibodies against STAP-1 (rabbit polyclonal) were purchased from Sigma-Aldrich.

    Techniques: Derivative Assay, Mouse Assay, Expressing, Quantitative RT-PCR, Flow Cytometry, Cell Culture, Irradiation, Transplantation Assay

    STAP-1 regulates apoptosis in CML LSCs. LSCs (GFP + LSK cells) in BM of primary WT or STAP-1 KO CML mice were analyzed at day 11 after first BMT. a Sorted LSCs were cultured in methylcellulose medium for 7 days, colonies were counted, and cells were serially replated ( n = 10 per group). b The cell cycle status of LSCs was analyzed by flow cytometry using Ki67 staining ( n = 7 per group). c BrdU was administered to WT or STAP-1 KO CML mice in in vivo as described in Material and Methods to assess the cell cycle status of GFP + Lin − c-Kit + cells with flow cytometry ( n = 7 per group). d Cell apoptosis was analyzed by Annexin V and 7AAD in GFP + cells or GFP + LSK cells from primary WT or STAP-1 KO CML mice using flow cytometry. Representative flow cytometric data of the apoptosis are shown in left panel. The numbers in the plot indicate mean percentages ± SD of gated cells. Bar graph represents mean proportion of Annexin V + cells ( n = 7 per group). e Cell apoptosis was analyzed by TUNEL staining. Sorted LSCs were fixed and stained with TUNEL (red) and DAPI (blue). Bar graphs represent the proportion of TUNEL-positive cells. Data are representative of one of the two independent experiments with similar results. ** p

    Journal: Oncogene

    Article Title: Signal-transducing adapter protein-1 is required for maintenance of leukemic stem cells in CML

    doi: 10.1038/s41388-020-01387-9

    Figure Lengend Snippet: STAP-1 regulates apoptosis in CML LSCs. LSCs (GFP + LSK cells) in BM of primary WT or STAP-1 KO CML mice were analyzed at day 11 after first BMT. a Sorted LSCs were cultured in methylcellulose medium for 7 days, colonies were counted, and cells were serially replated ( n = 10 per group). b The cell cycle status of LSCs was analyzed by flow cytometry using Ki67 staining ( n = 7 per group). c BrdU was administered to WT or STAP-1 KO CML mice in in vivo as described in Material and Methods to assess the cell cycle status of GFP + Lin − c-Kit + cells with flow cytometry ( n = 7 per group). d Cell apoptosis was analyzed by Annexin V and 7AAD in GFP + cells or GFP + LSK cells from primary WT or STAP-1 KO CML mice using flow cytometry. Representative flow cytometric data of the apoptosis are shown in left panel. The numbers in the plot indicate mean percentages ± SD of gated cells. Bar graph represents mean proportion of Annexin V + cells ( n = 7 per group). e Cell apoptosis was analyzed by TUNEL staining. Sorted LSCs were fixed and stained with TUNEL (red) and DAPI (blue). Bar graphs represent the proportion of TUNEL-positive cells. Data are representative of one of the two independent experiments with similar results. ** p

    Article Snippet: Antibodies against STAP-1 (rabbit polyclonal) were purchased from Sigma-Aldrich.

    Techniques: Mouse Assay, Cell Culture, Flow Cytometry, Staining, In Vivo, TUNEL Assay

    STAP-1 deficiency improves OS in in vivo CML mouse model. a BCR-ABL-transduced 293T cells were either or not co-transfected with Myc-tagged STAP-1. The cells were lysed, immunoprecipitated with anti-Myc antibody, and blotted with anti-ABL (upper panel) or anti-STAP-1 antibody (middle panel). An aliquot of total cell lysate (TCL) was also blotted with anti-ABL antibody (lower panel). b Experimental strategy for generation of CML mouse model. BCR-ABL-transduced LSK cells (1 × 10 4 cells, Ly5.2) were transplanted into lethally irradiated recipients (Ly5.1). For serial transplantation, GFP + BM cells or GFP + CD45.2 + LSK cells from primary CML mice were isolated at day 11 after first BMT, and transplanted into lethally irradiated secondary recipient mice (Ly5.1). c Kaplan–Meier survival curves for primary and secondary recipients of BCR-ABL-transduced cells from WT or STAP-1 KO donor mice are shown. d The total numbers of peripheral white blood cells and GFP + CD45.2 + cells in BM were analyzed at day 11 after first BMT (WT, n = 13; STAP-1 KO, n = 11). e Gross appearance of the lungs and spleens at day 11 after first BMT is shown. Bar graph represents the spleen weight of primary CML mice (WT, n = 11; STAP-1 KO, n = 12). f Leukemic stem cells (LSCs; GFP + LSK cells) in BM of primary CML mice were analyzed at day 11 after first BMT. The proportions and total numbers of CML LSCs in BM were calculated (WT, n = 13; STAP-1 KO, n = 11). Data are shown as mean ± SD. * p

    Journal: Oncogene

    Article Title: Signal-transducing adapter protein-1 is required for maintenance of leukemic stem cells in CML

    doi: 10.1038/s41388-020-01387-9

    Figure Lengend Snippet: STAP-1 deficiency improves OS in in vivo CML mouse model. a BCR-ABL-transduced 293T cells were either or not co-transfected with Myc-tagged STAP-1. The cells were lysed, immunoprecipitated with anti-Myc antibody, and blotted with anti-ABL (upper panel) or anti-STAP-1 antibody (middle panel). An aliquot of total cell lysate (TCL) was also blotted with anti-ABL antibody (lower panel). b Experimental strategy for generation of CML mouse model. BCR-ABL-transduced LSK cells (1 × 10 4 cells, Ly5.2) were transplanted into lethally irradiated recipients (Ly5.1). For serial transplantation, GFP + BM cells or GFP + CD45.2 + LSK cells from primary CML mice were isolated at day 11 after first BMT, and transplanted into lethally irradiated secondary recipient mice (Ly5.1). c Kaplan–Meier survival curves for primary and secondary recipients of BCR-ABL-transduced cells from WT or STAP-1 KO donor mice are shown. d The total numbers of peripheral white blood cells and GFP + CD45.2 + cells in BM were analyzed at day 11 after first BMT (WT, n = 13; STAP-1 KO, n = 11). e Gross appearance of the lungs and spleens at day 11 after first BMT is shown. Bar graph represents the spleen weight of primary CML mice (WT, n = 11; STAP-1 KO, n = 12). f Leukemic stem cells (LSCs; GFP + LSK cells) in BM of primary CML mice were analyzed at day 11 after first BMT. The proportions and total numbers of CML LSCs in BM were calculated (WT, n = 13; STAP-1 KO, n = 11). Data are shown as mean ± SD. * p

    Article Snippet: Antibodies against STAP-1 (rabbit polyclonal) were purchased from Sigma-Aldrich.

    Techniques: In Vivo, Transfection, Immunoprecipitation, Irradiation, Transplantation Assay, Mouse Assay, Isolation