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Image Search Results
Journal: American Journal of Physiology - Renal Physiology
Article Title: Differences in renal BMAL1 contribution to Na + homeostasis and blood pressure control in male and female mice
doi: 10.1152/ajprenal.00014.2020
Figure Lengend Snippet: Antibody information
Article Snippet: Antibody information is shown in . table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Antibody Target Manufacturer Catalog Number Reference Description Dilution Used
Techniques: Western Blot
Journal: American Journal of Physiology - Renal Physiology
Article Title: Differences in renal BMAL1 contribution to Na + homeostasis and blood pressure control in male and female mice
doi: 10.1152/ajprenal.00014.2020
Figure Lengend Snippet: Characterization of kidney-specific brain and muscle ARNT-like 1 (BMAL1) knockout (KS-BMAL1 KO) mice. A: model showing exon 8 of BMAL1 excision by Ksp-cadherin Cre. Solid gray arrows represent primers used for recombination PCR. *Band produced from the recombined floxed gene. B: recombination PCR assay showing the expected 570-bp product (*) in renal inner medullary tissue of KS-BMAL1 KO mice but not control (CNTL) mice. C: additional assays assessing nonrenal tissue revealing the lack of the KO band. D−I: representative images of BMAL1 protein expression in renal segments. D, F, and H: colabeling with BMAL1 (brown) in CNTL mice; E, G, and I: colabeling with BMAL1 (brown) in KS-BMAL1 KO mice. D and E: blue staining with Na+-K+-2Cl− cotransporter 2 (NKCC2) representing the cortical thick ascending limb (TAL). F and G: blue staining with phosphorylated Na+-Cl− cotransporter (p-NCC) to identify distal convoluted tubules (DCTs). H and I: blue staining with aquaporin 2 representing medullary collecting duct (CD) cells. Both PCR and immunohistochemistry images are from male tissue except for ovary tissue from female mice. Arrows indicate BMAL1-positive stained nuclei; arrowheads indicate nuclei with negative BMAL1 staining. J: immunoblots of renal inner medullary homogenates from male mice to demonstrate BMAL1 protein expression and total protein from Ponceau staining. The predicted size of BMAL1 band (78 kDa) is noted. K: quantification determined by normalizing BMAL1 values with total protein. PT, proximal tubule. Values are means ± SE; n = 4. Student’s t test was used to compare genotypes. Scale bar = 0.05 mm.
Article Snippet: Antibody information is shown in . table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Antibody Target Manufacturer Catalog Number Reference Description Dilution Used
Techniques: Knock-Out, Produced, Expressing, Staining, Immunohistochemistry, Western Blot
Journal: American Journal of Physiology - Renal Physiology
Article Title: Differences in renal BMAL1 contribution to Na + homeostasis and blood pressure control in male and female mice
doi: 10.1152/ajprenal.00014.2020
Figure Lengend Snippet: Representative images of brain and muscle ARNT-like 1 (BMAL1) expression in different regions of the kidney from male kidney-specific BMAL1 knockout (KS-BMAL1 KO) mouse sections. A–H: colabeling with BMAL1 (brown) and Na+-K+-2Cl− cotransporter (NKCC2; A–D) or aquaporin 2 (E–H) in blue stain. NKCC2- and aquaporin 2-positive stained cells efficiently lacked BMAL1 expression in KS-BMAL1 KO mice. G: afferent and efferent arterioles (arrowheads) of a glomerulus. H: medullary vascular bed containing vasa recta capillaries (VR). The macula densa (MD) was positive for BMAL1. CD, collecting duct; IC, intercalated cells; PT, proximal tubule; TAL, thick ascending limb. Scale bar = 0.05 mm.
Article Snippet: Antibody information is shown in . table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Antibody Target Manufacturer Catalog Number Reference Description Dilution Used
Techniques: Expressing, Knock-Out, Staining